CN106676008A - Large-scale farming culture medium of spirulina platensis - Google Patents
Large-scale farming culture medium of spirulina platensis Download PDFInfo
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- CN106676008A CN106676008A CN201510753599.0A CN201510753599A CN106676008A CN 106676008 A CN106676008 A CN 106676008A CN 201510753599 A CN201510753599 A CN 201510753599A CN 106676008 A CN106676008 A CN 106676008A
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- culture medium
- scale farming
- farming culture
- spirulina platensis
- spirulina
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Abstract
The invention belongs to the field of microalgae cultivation, and particularly relates to a large-scale farming culture medium for spirulina platensis. The large-scale farming culture medium is prepared from the following formula components: 0.3 to 0.7 g of urea, 6 to 10 g of sodium bicarbonate, 0.15 to 0.25 g of dipotassium phosphate, 0.15 to 0.25 g of monopotassium phosphate, 0.4 to 0.6 g of sodium chloride, 0.2 to 0.3 g of potassium chloride, 0.01 to 0.03 g of ferric ammonium citrate, 0.04 to 0.06 g of disodium EDTA (Ethylene Diamine Tetraacetate), 10 ml of soil extract and 1000 ml of deionized water. Various N sources are supplemented, and sufficient nutrition is provided for algae; by adding a phosphoric acid buffer system, the pH (Potential of Hydrogen) value of an algae solution is stabilized; by using the soil extract to replace various trace elements, the operation is simple, the use is convenient, and the production cost is greatly saved; by using the large-scale farming culture medium disclosed by the invention to culture the spirulina platensis, the growth speed of strains is fast, the breeding cycle is short, and the cell density is high.
Description
Technical field
The invention belongs to field of microalgae cultivation, more particularly to a kind of spirulina plalensiss large-scale farming culture medium.
Background technology
Spirulina (Spirulina) be also " arthrospira ", belongs to Cyanophyceae, Oscillariaceae.Frond is constituted for single-row cell
Not branch filamentous, keratinized sheath without or only very thin sheath, and regular helical form, to form hormocystangium breeding.Spiral shell
Rotation algae protein content is high, and fat, content of cellulose are low, also containing miscellaneous vitamin.Additionally, it or institute
There is absorbability irony content highest in food, while also find that it has anti-cancer, the algae protein of effect for cancer, with
And the bioactive substance of other a large amount of mineral elements and raising immunity of organisms.Therefore, spirulina breeding industry is at home
Development is growing steadily, and become health products trade gives priority to object.
Cultivated from Zarrouk culture medium more than spirulina plalensiss at present, its culture efficiency is more obvious in the lab,
But be then not reaching to preferable effect in large-scale culture, main cause be N sources undernutrition, pH easily raise, it is micro-
Secondary element improves production cost etc..
The content of the invention
To solve the problems, such as current techniques, we provide a kind of spirulina plalensiss large-scale farming culture medium.
The concrete technical scheme that the present invention is adopted：
Spirulina plalensiss large-scale farming culture medium, formula components and content it is as follows：0.3～0.7g of carbamide, sodium bicarbonate 6～
10g, 0.15～0.25g of dipotassium hydrogen phosphate, 0.15～0.25g of potassium dihydrogen phosphate, 0.4～0.6g of Sodium Chloride, potassium chloride 0.2～
0.3g, 0.01～0.03g of ferric ammonium citrate, 0.04～0.06g of disodiumedetate, soil extract 10ml, go from
Sub- water 1000ml.
The easy photodissociation of the ferric ammonium citrate, need to be made into mother solution, keep in dark place in brown bottle.
Beneficial effects of the present invention：
By the N sources for supplementing various ways, enough nutrition is provided for Growth of Spirulina Platensis；By adding phosphoric acid buffer
System, can stablize algae solution pH value, prevent pH from raising the algae strain for causing dead；Substituted by using soil extract various
Trace element, it is simple to operate, easy to use, it is greatly saved production cost.Using present invention culture spirulina plalensiss,
Algae strain growth rate is fast, the breeding cycle is short, cell density is high.
Specific embodiment
With reference to specific embodiment, the present invention will be described in detail：
Embodiment 1
Spirulina plalensiss large-scale farming culture medium, formula components and content it is as follows：Carbamide 0.3g, sodium bicarbonate 6g, phosphorus
Sour hydrogen dipotassium 0.15g, potassium dihydrogen phosphate 0.15g, Sodium Chloride 0.4g, potassium chloride 0.2g, ferric ammonium citrate 0.01g, second two
Amine tetraacethyl disodium 0.04g, soil extract 10ml, deionized water 1000ml.
The easy photodissociation of the ferric ammonium citrate, need to be made into mother solution, keep in dark place in brown bottle.
Embodiment 2
Spirulina plalensiss large-scale farming culture medium, formula components and content it is as follows：Carbamide 0.7g, sodium bicarbonate 10g,
Dipotassium hydrogen phosphate 0.25g, potassium dihydrogen phosphate 0.25g, Sodium Chloride 0.6g, potassium chloride 0.3g, ferric ammonium citrate 0.03g, second
Edetate disodium 0.06g, soil extract 10ml, deionized water 1000ml.
The easy photodissociation of the ferric ammonium citrate, need to be made into mother solution, keep in dark place in brown bottle.
Embodiment 3
Spirulina plalensiss large-scale farming culture medium, formula components and content it is as follows：Carbamide 0.5g, sodium bicarbonate 8g, phosphorus
Sour hydrogen dipotassium 0.2g, potassium dihydrogen phosphate 0.2g, Sodium Chloride 0.5g, potassium chloride 0.25g, ferric ammonium citrate 0.02g, second two
Amine tetraacethyl disodium 0.05g, soil extract 10ml, deionized water 1000ml.
The easy photodissociation of the ferric ammonium citrate, need to be made into mother solution, keep in dark place in brown bottle.
Above are only several specific embodiments of the present invention, it is clear that the invention is not restricted to above example, can also have
Many deformations.All deformations that one of ordinary skill in the art can directly derive from present disclosure or associate,
Protection scope of the present invention is considered as.
Claims (2)
1. a kind of spirulina plalensiss large-scale farming culture medium, it is characterised in that formula components and content are as follows：Carbamide
0.3～0.7g, 6～10g of sodium bicarbonate, 0.15～0.25g of dipotassium hydrogen phosphate, 0.15～0.25g of potassium dihydrogen phosphate, chlorine
Change 0.4～0.6g of sodium, 0.2～0.3g of potassium chloride, 0.01～0.03g of ferric ammonium citrate, disodiumedetate 0.04～
0.06g, soil extract 10ml, deionized water 1000ml.
2. spirulina plalensiss large-scale farming culture medium according to claim 1, it is characterised in that the ferric citrate
The easy photodissociation of ammonium, need to be made into mother solution, keep in dark place in brown bottle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510753599.0A CN106676008A (en) | 2015-11-06 | 2015-11-06 | Large-scale farming culture medium of spirulina platensis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510753599.0A CN106676008A (en) | 2015-11-06 | 2015-11-06 | Large-scale farming culture medium of spirulina platensis |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106676008A true CN106676008A (en) | 2017-05-17 |
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ID=58863060
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510753599.0A Pending CN106676008A (en) | 2015-11-06 | 2015-11-06 | Large-scale farming culture medium of spirulina platensis |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106676008A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108179124A (en) * | 2018-01-13 | 2018-06-19 | 陈金华 | A kind of complex medium for being used to cultivate blunt top spirulina |
| CN113502226A (en) * | 2021-08-31 | 2021-10-15 | 西藏大学 | Culture medium for separating, purifying and amplifying culture of plateau microalgae |
-
2015
- 2015-11-06 CN CN201510753599.0A patent/CN106676008A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108179124A (en) * | 2018-01-13 | 2018-06-19 | 陈金华 | A kind of complex medium for being used to cultivate blunt top spirulina |
| CN113502226A (en) * | 2021-08-31 | 2021-10-15 | 西藏大学 | Culture medium for separating, purifying and amplifying culture of plateau microalgae |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170517 |