CN106669423A - Molecular gradient forward and reverse ultra-filtration separation and purification device and ultra-filtration method thereof - Google Patents
Molecular gradient forward and reverse ultra-filtration separation and purification device and ultra-filtration method thereof Download PDFInfo
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- CN106669423A CN106669423A CN201611140568.9A CN201611140568A CN106669423A CN 106669423 A CN106669423 A CN 106669423A CN 201611140568 A CN201611140568 A CN 201611140568A CN 106669423 A CN106669423 A CN 106669423A
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- blocking device
- ultra
- filtration
- reverse
- filtration unit
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/18—Apparatus therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
- B01D61/145—Ultrafiltration
- B01D61/146—Ultrafiltration comprising multiple ultrafiltration steps
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D63/00—Apparatus in general for separation processes using semi-permeable membranes
- B01D63/06—Tubular membrane modules
- B01D63/062—Tubular membrane modules with membranes on a surface of a support tube
- B01D63/063—Tubular membrane modules with membranes on a surface of a support tube on the inner surface thereof
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2311/00—Details relating to membrane separation process operations and control
- B01D2311/26—Further operations combined with membrane separation processes
- B01D2311/2676—Centrifugal separation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2313/00—Details relating to membrane modules or apparatus
- B01D2313/04—Specific sealing means
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2313/00—Details relating to membrane modules or apparatus
- B01D2313/13—Specific connectors
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2317/00—Membrane module arrangements within a plant or an apparatus
- B01D2317/02—Elements in series
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D2317/00—Membrane module arrangements within a plant or an apparatus
- B01D2317/06—Use of membrane modules of the same kind
Abstract
The invention discloses a molecular gradient forward and reverse ultra-filtration separation and purification device and a use method thereof. The device comprises an end capping device and at least two ultra-filtration units, wherein each of the ultra-filtration units comprises a tube and an ultra-filtration film; the ultra-filtration film is arranged along a radial direction in the tube; the adjacent ultra-filtration units are detachably connected with each other along an axial direction; the maximal passing molecular weights of the ultra-filtration films of the adjacent ultra-filtration units are different from each other; and the ultra-filtration units at the two ends are respectively connected with the end capping device. According to the invention, the molecular gradient-based closed type automatic separation and purification for the bio-molecules in different molecular weights in different regions can be realized, the forward and reverse repeated purification can be conveniently realized, and the bio-molecules in related regions can be conveniently collected in gradient.
Description
Technical field
The present invention relates to biological technical field, more particularly to molecule ultra-filtration and separation purification art.
Background technology
As the biomacromolecule materials such as the development of biotechnology, small protein, polysaccharide or nucleic acid play important life
Thing effect is acted on, and Most scholars are devoted to the research of association area.In order to accurately study the effect machine of a certain class biomolecule
Reason, it is necessary to be first isolated purification, so as to realize orienting the purpose of accurate research.For this reason, it may be necessary to invent a kind of device can be by
The bio-molecular separation of different molecular weight size comes, at present, a kind of product Amicon ultra-0.5 of existing commercialization
Centrifugal filter devices (made in iceland) can will be greater than or less than the biology point of certain molecular weight
Son is separated, i.e. employing can pass through the filter membrane (such as 10000) of the biomolecule of certain molecular weight, and mixed liquor is added into filter membrane
In the device of top, it is centrifuged, by filter membrane, the molecule more than 10000 is adsorbed to biomolecule of the molecular weight less than 10000
On filter membrane, so as to reach detached purpose, researcher can be analyzed with the protein molecular on eluting filter membrane (being more than 10000),
Or the filtrate drawn below filter membrane is further analyzed (less than 10000).But generally researcher is needed between some region of biology
Molecule is analyzed, such as the molecule between 5000~10000, and researcher needs to collect filter membrane lower section solution, then row passes through
The filter membrane of 5000 molecular weight, eluting collects the biomolecule that the biomolecule on filter membrane is 5000 to 10000.
Prior art is not enough：
(1) biomolecule of a certain interval molecular weight is needed to need to operate twice, complex operation, and biomolecule loss
Amount is big, and yield is low；
(2) biomolecule of multiple interval molecular weight can not simultaneously be obtained；
(3) incorporation for reducing other molecular weight biological macromole to try one's best, needs multiple repeatable operation, wastes time and energy,
Solution repeatedly shifts a large amount of losses that will cause biological molecules of interest, and the waste of research purpose biomacromolecule is likely to result in reality
Test termination or time delay.
The content of the invention
The present invention is intended to provide a kind of forward and reverse ultra-filtration and separation purification devices of molecular grade, can realize close by molecular grade
Enclosed is automatically separated the biomolecule of the different interval molecular weight of purification, conveniently realizes forward and reverse multiple purification, is easy to classification to collect
The biomolecule of related interval.
To reach above-mentioned purpose, the technical solution used in the present invention is as follows：
The forward and reverse ultra-filtration and separation purification devices of molecular grade disclosed by the invention, including end-blocking device, at least two ultrafiltration lists
Unit, the ultra filtration unit includes body, ultrafilter membrane, and ultrafilter membrane, edge between adjacent ultra filtration unit are disposed radially in the body
Axially it is detachably connected, the aperture of adjacent ultra filtration unit ultrafilter membrane is different, the ultra filtration unit at two ends is detachable with end-blocking device respectively
Connection.
Preferably, one end of the body is provided with female thread a, and the other end of body is provided with external screw thread a, the end-blocking device bag
The first end-blocking device, the second end-blocking device are included, the first end-blocking device is provided with external screw thread b, and the second end-blocking device is provided with female thread b.
Preferably, the first end-blocking device is drum dress, and the external screw thread b is located at the opening of the first end-blocking device, described
Second end-blocking device is drum dress, and the female thread b is located at the opening of the second end-blocking device.
Further, the female thread a bottoms, female thread b bottoms are respectively equipped with sealing ring.
Preferably, the ultra filtration unit, the first end-blocking device, the second end-blocking device internal diameter it is identical, the ultra filtration unit, first
End-blocking device, second end-blocking device external diameter it is identical.
The ultrafilter membrane is also referred to as semipermeable membrane.
Using the hyperfiltration process of the forward and reverse ultra-filtration and separation purification devices of molecular grade, comprise the following steps：
A. molecular grade forward direction ultra-filtration and separation：Be connected in series between ultra filtration unit, two ends connect respectively the first end-blocking device,
Two end-blocking devices, take the first end-blocking device or the second end-blocking device apart, add solution, reconnect first and block device or the second end-blocking device, enter
Row centrifugation.
Preferably, in step a, the aperture of the plurality of ultra filtration unit ultrafilter membrane from top to bottom is sequentially reduced, solution
Add from upper end.Specifically, first solution poured in the first end-blocking device, then the above-mentioned ultra filtration unit for connecting second is blocked into device
Overall inversion is connected with the first end-blocking device, and then can turn around carries out positive centrifugation.Different amount of solution may be selected not
The end-blocking device of same depth (volume), to change its capacity.
Further, it is further comprising the steps of after step a：
B. the reverse ultrafiltration purification of molecular grade：The first end-blocking device or the second end-blocking device of upper end are removed, continues to connect ultrafiltration
Unit, the aperture of the multiple ultra filtration units added afterwards ultrafilter membrane from bottom to up is sequentially reduced, and topmost connects the first envelope pulled down
End-apparatus or the second end-blocking device, pull down the first end-blocking device or the second end-blocking device of bottom, outwell solution, add eluent, then with
The ultra filtration unit connection of bottom, then turned upside down carries out centrifugal purification.
Preferably, in step b, the first end-blocking device or the second end-blocking device of bottom is pulled down, outwells solution, addition is washed
De- liquid, then be connected with the ultra filtration unit of bottom, then turned upside down is centrifuged repeatedly, is capable of achieving to improve different
Biomolecule purity between molecular weight area.
The invention has the advantages that：
1. the present invention can realize isolating and purifying the biomolecule of different interval molecular weight by molecular grade；
2. the present invention can reverse multiple purification, remove the biomolecule of the relatively small molecular weight that residual is mixed；
3. the present invention is closed, and multi-pass operation is without changing container, the loss of inanimate object molecule；
4. the present invention is closed, and the harm risk that solution is caused to operator is little；
5. the present invention can as needed carry out the assembling of multiple combination (including different molecular weight combination, different solutions capacity
Combination etc.), the suitability is wide.
Description of the drawings
Fig. 1 is the structural representation of invention；
Fig. 2 is the axial sectional view of the present invention in Fig. 1；
Fig. 3 is the schematic diagram of assembling device of the present invention；
Fig. 4 is the schematic diagram that the present invention is assembled again；
In figure：Outside 1- ultra filtration units, the end-blocking devices of 2- first, the end-blocking devices of 3- second, 11- female thread a, 12- ultrafilter membranes, 13-
Screw thread a, 21- external screw thread b, 31- female thread b, 101- ultra filtration unit h, 102- ultra filtration unit g, 103- ultra filtration unit f, 104- surpasses
Filter unit e, 105- ultra filtration unit a, 106- ultra filtration unit b, 107- ultra filtration unit c, 108- ultra filtration unit d.
Specific embodiment
In order that the objects, technical solutions and advantages of the present invention become more apparent, below in conjunction with accompanying drawing, the present invention is entered
Row is further described.
As shown in Figure 1-2, the forward and reverse ultra-filtration and separation purification devices of molecular grade disclosed by the invention, including end-blocking device, extremely
Few two ultra filtration units 1, ultra filtration unit 1 includes body, ultrafilter membrane 12, ultrafilter membrane 12, adjacent ultrafiltration is disposed radially in body
It is detachably connected vertically between unit 1, the aperture (maximum can pass through molecular weight) of the ultrafilter membrane 12 of adjacent ultra filtration unit 1 is no
Together, the ultra filtration unit 1 at two ends is connected respectively with end-blocking device, and one end of body is provided with female thread a11, and the other end of body is provided with outer
Screw thread a13, end-blocking device includes the first end-blocking end-blocking device 3 of device 2, second, and the first end-blocking device 2 is provided with external screw thread b21, the second end-blocking device
3 are provided with female thread b31, and the first end-blocking device 2 is drum dress, and external screw thread b21 is located at the opening of the first end-blocking device 2, the second end-blocking
Device 3 is drum dress, and female thread b31 is located at the opening of the second end-blocking device 3, and ultrafilter membrane 12 can be by molecular mass for maximum
1000th, the ultrafilter membrane of 3000,5000,10000 dalton, female thread a11 bottoms, female thread b31 bottoms are respectively equipped with sealing ring,
The internal diameter of the end-blocking end-blocking device 3 of device 2, second of ultra filtration unit 1, first is identical, the end-blocking end-blocking device 3 of device 2, second of ultra filtration unit 1, first
External diameter it is identical.
As shown in Figure 3-4, the using method of the forward and reverse ultra-filtration and separation purification devices of molecular grade, comprises the following steps：
A. molecular grade forward direction ultra-filtration and separation：By ultra filtration unit a105, ultra filtration unit b106, ultra filtration unit c107, ultrafiltration
Unit d108 is sequentially connected from top to bottom, and upper end connection first blocks device 2, and lower end connection second blocks device 3, wherein ultra filtration unit
A105, ultra filtration unit b106, ultra filtration unit c107, ultra filtration unit d108 maximum can be respectively 10000 by molecular mass,
5000th, 3000,1000 dalton, as shown in Figure 3；Take the first end-blocking device 2 apart, add solution, reconnect first and block device 2, enter
Row centrifugation；Or according to amount of solution number pour the first end-blocking device 2 into, turn connection around, then just coming centrifugation；
B. the reverse ultrafiltration purification of molecular grade：The first end-blocking device 2 is removed, in ultra filtration unit a105 upper end from bottom to top successively
Connection ultra filtration unit e104, ultra filtration unit f103, ultra filtration unit g102, ultra filtration unit h101, topmost connect first for pulling down
End-blocking device 2, wherein ultra filtration unit e104, ultra filtration unit f103, ultra filtration unit g102, ultra filtration unit h101 maximum can pass through molecule
Quality is respectively 10000,5000,3000,1000 dalton, as shown in Figure 4；The second end-blocking device 3 is pulled down, solution is outwelled, is added
Eluent, then be connected with ultra filtration unit d108, then turned upside down is centrifuged purification.
Certainly to lift purification effect, in step b, after step b terminates, the first end-blocking device 2 can be pulled down, outwells solution,
Eluent is added, then is connected with ultra filtration unit mesh h101, then turned upside down is centrifuged, and step b can be repeated several times, and carries
Rise purification effect.
Certainly, the present invention can also have other various embodiments, ripe in the case of without departing substantially from spirit of the invention and its essence
Knowing those skilled in the art can make various corresponding changes and deformation, but these corresponding changes and deformation according to the present invention
The protection domain of appended claims of the invention should all be belonged to.
Claims (9)
1. forward and reverse ultra-filtration and separation purification devices of molecular grade, it is characterised in that：Including end-blocking device, at least two ultra filtration units
(1), the ultra filtration unit (1) includes body, ultrafilter membrane (12), and ultrafilter membrane (12) is disposed radially in the body, adjacent super
It is detachably connected vertically between filter unit (1), the aperture of adjacent ultra filtration unit (1) ultrafilter membrane (12) is different, the ultrafiltration at two ends
Unit (1) is detachably connected respectively with end-blocking device.
2. forward and reverse ultra-filtration and separation purification devices of molecular grade according to claim 1, it is characterised in that：The body
One end is provided with female thread a (11), and the other end of body is provided with external screw thread a (13), the end-blocking device include the first end-blocking device (2),
Second end-blocking device (3), first end-blocking device (2) is provided with external screw thread b (21), and second end-blocking device (3) is provided with female thread b
(31)。
3. forward and reverse ultra-filtration and separation purification devices of molecular grade according to claim 2, it is characterised in that：First envelope
End-apparatus (2) is drum dress, and the external screw thread b (21) is located at the opening of the first end-blocking device (2), and second end-blocking device (3) is
Drum is filled, and the female thread b (31) is located at the opening of the second end-blocking device (3).
4. forward and reverse ultra-filtration and separation purification devices of molecular grade according to claim 1, it is characterised in that：The female thread
A (11) bottom, female thread b (31) bottom are respectively equipped with sealing ring.
5. forward and reverse ultra-filtration and separation purification devices of molecular grade according to claim 1, it is characterised in that：The ultrafiltration list
First (1), the first end-blocking device (2), the second end-blocking device (3) internal diameter it is identical, the ultra filtration unit (1), the first end-blocking device (2), the
The external diameter of two end-blockings device (3) is identical.
6. usage right requires the hyperfiltration process of the forward and reverse ultra-filtration and separation purification devices of molecular grade described in 1-5 any one,
It is characterized in that comprising the following steps：
A. molecular grade forward direction ultra-filtration and separation：Ultra filtration unit is connected in series between (1), two ends connect respectively the first end-blocking device (2),
Second end-blocking device (3), take apart the first end-blocking device (2) or second end-blocking device (3), add solution, reconnect first block device (2) or
Second end-blocking device (3), is centrifuged.
7. hyperfiltration process according to claim 6, it is characterised in that：In step a, the plurality of ultra filtration unit (1)
From top to bottom the aperture of ultrafilter membrane (12) is sequentially reduced, and solution is added from upper end.
8. hyperfiltration process according to claim 7, it is characterised in that：It is further comprising the steps of after step a：
B. the reverse ultrafiltration purification of molecular grade：First end-blocking device (2) or the second end-blocking device (3) of upper end are removed, continues to connect super
Filter unit (1), the aperture of the multiple ultra filtration units (1) added afterwards ultrafilter membrane (12) from bottom to up is sequentially reduced, and topmost connects
New the first end-blocking device (2) or second blocks device (3), pulls down first end-blocking device (2) or the second end-blocking device (3) of bottom,
Fall solution, add eluent, then be connected with the ultra filtration unit (1) of bottom, then turned upside down carries out centrifugal purification.
9. hyperfiltration process according to claim 8, it is characterised in that：In step b, the first end-blocking of bottom is pulled down
Device (2) or the second end-blocking device (3), outwell solution, add eluent, then are connected with the ultra filtration unit (1) of bottom, then up and down
Inversion is centrifuged repeatedly, is capable of achieving to improve the interval biomolecule purity of different molecular weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611140568.9A CN106669423B (en) | 2016-12-12 | 2016-12-12 | The forward and reverse ultra-filtration and separation purification devices of molecular grade and its hyperfiltration process |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611140568.9A CN106669423B (en) | 2016-12-12 | 2016-12-12 | The forward and reverse ultra-filtration and separation purification devices of molecular grade and its hyperfiltration process |
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| Publication Number | Publication Date |
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| CN106669423A true CN106669423A (en) | 2017-05-17 |
| CN106669423B CN106669423B (en) | 2019-05-03 |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103614339A (en) * | 2013-11-22 | 2014-03-05 | 武汉友芝友医疗科技有限公司 | Cell separation method |
| CN205495355U (en) * | 2016-04-13 | 2016-08-24 | 吉林大学 | Automatic control continuous feeding multi -stage filtration's ultrafiltration device |
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2016
- 2016-12-12 CN CN201611140568.9A patent/CN106669423B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103614339A (en) * | 2013-11-22 | 2014-03-05 | 武汉友芝友医疗科技有限公司 | Cell separation method |
| CN205495355U (en) * | 2016-04-13 | 2016-08-24 | 吉林大学 | Automatic control continuous feeding multi -stage filtration's ultrafiltration device |
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| CN106669423B (en) | 2019-05-03 |
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