CN106661589A - 在植物中产生二倍体配子的tdm基因中的显性突变 - Google Patents

在植物中产生二倍体配子的tdm基因中的显性突变 Download PDF

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CN106661589A
CN106661589A CN201580023921.2A CN201580023921A CN106661589A CN 106661589 A CN106661589 A CN 106661589A CN 201580023921 A CN201580023921 A CN 201580023921A CN 106661589 A CN106661589 A CN 106661589A
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拉斐尔·梅希尔
玛尔塔·西富恩特斯
劳伦斯·克鲁泽
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Abstract

本发明涉及：在TDM基因中的显性突变，该显性突变使得在植物中产生2n配子；包含所述突变的植物；以及，它们在植物育种中的应用。本发明还涉及这样的植物，在该植物中，TDM基因中的显性突变与以下基因的失活组合：参与植物中的减数分裂重组的基因，和参与减数分裂期间着丝粒的单极取向的基因。这些产生未减数分裂配子的植物也可用于植物育种。

Description

在植物中产生二倍体配子的TDM基因中的显性突变
技术领域
本发明涉及：引起在植物中产生2n配子的TDM基因中的显性突变；包含所述突变的植物；以及它们在植物育种中的应用。本发明还涉及这样的植物，在该植物中，TDM基因中的显性突变与以下基因的失活组合：参与植物中减数分裂重组的基因，和参与减数分裂期间着丝粒的单极取向的基因。这些产生未减数分裂配子的植物也可用于植物育种。
背景技术
减数分裂是有性繁殖的真核生物(诸如，大多数的开花植物)生命周期中的关键步骤。
在正常的减数分裂中，染色体首先复制，产生成对的姐妹染色单体。在该轮复制之后，进行两轮分裂，称为减数第一次分裂和减数第二次分裂。在减数第一次分裂期间，同源染色体重组并分离在两个细胞隔室(compartiment)中，这个两个细胞隔室各自都包含染色体的一个完整的单倍体内容物。在减数第二次分裂中，由减数第一次分裂产生的两组染色体进一步分裂，使姐妹染色单体分离开。因此，由该分裂产生的四个孢子是单倍体(n)，并且携带重组的遗传信息。
相比之下，在二倍体细胞的有丝分裂期间，染色体进行复制，姐妹染色单体分离产生子代细胞，该子代细胞是二倍体(2n)的并且在遗传学上与原始细胞相同。
已经示出，由减数分裂期间的异常现象得到的异常配子可用于几种感兴趣植物(包括作物)的遗传改良(供参考，参见例如，Ramanna&Jacobsen,Euphytica 2003,133,3-18)。具体地，2n配子和未减数分裂配子(apomeiotic gamete)可用于产生多倍体植物，或用于使不同倍性水平的植物，例如四倍体作物与其二倍体野生近缘种杂交，以便在植物育种方法中使用它们的遗传多样性。它们也可用于遗传作图的方法。未减数分裂配子对于产生无融合生殖植物(即能够从胚珠的母体组织形成种子的植物)也是感兴趣的，从而产生作为母本的遗传克隆的子代。
2n配子(也称为二倍体配子)是具有体细胞染色体数，而不是配子体染色体数的配子。引起2n配子形成的异常情况特别包括异常胞质分裂，第一次减数分裂或第二次减数分裂的遗漏(skip)，或纺锤体异形(供参考，参见V Veilleux,Plant Breeding Reviews,1985,3,252-288,or Bretagnolle&Thompson,New Phytologist,1995,129,1-22)。这些异常情况导致不同类别的未减数的配子。例如，第一次减数分裂的遗漏产生第一次分裂恢复(FDR)的配子，而第二次减数分裂的缺少产生第二次分裂恢复(SDR)的配子。已经报道了在各种植物物种中能够产生2n配子的许多突变体。然而，还未对这些植物中参与二倍体配子形成的突变进行表征。
未减数分裂配子是遗传学上与原始细胞相同的配子，保留亲本的所有遗传信息。未减数分裂配子的产生是无融合生殖的关键因素之一(Bicknell&Koltunow，Plant Cell，2004,16，S228-45)。虽然，超过400种植物是无融合生殖的，但这些植物中包括很少的作物物种。此外，通过杂交来引入这一性状的尝试已失败了(“The Flowering of Apomixis:From Mechanisms to Genetic Engineering”,2001；Editor:Savidan et al.；Publisher:CIMMYT,IRD,European Commission DG VI(FAIR),MEXICO,2001.Spillane et al.,SexualPlant Reproduction,2001,14,179-187)。
迄今为止，已经证实了只有少数基因与2n或未减数分裂花粉的形成有关。
AtPS1(拟南芥平行纺锤体，Arabidopsis thaliana parallel spindles)的失活产生二倍雄孢子，从而产生具有重组遗传信息的可育的二倍花粉粒和在子代中产生自发三倍体植物(WO 2010/004431；d’Erfurth et al.,PLoS Genet.,2008,4,e1000274)。
TAM(缓慢异步的减数分裂，也称为CYCA1；2)或OSD1(第二次分裂的消失)的失活导致在减数第一次分裂之后过早地退出减数分裂，从而产生具有重组遗传信息的二倍体孢子和SDR配子(d’Erfuth et al.,PLoS Genet.,2010,6,e1000989；d’Erfuth et al.,PLoSBiol.,2009,7,e1000124；WO 2010/079432)。
SPO11-1编码植物中进行有效减数分裂重组所必需的蛋白，并且它的抑制消除重组和配对(Grelon et al.,Embo J.,2001,20,589-600)；而Rec8(At2g47980)编码着丝粒在减数分裂期间的单极取向所必需的蛋白(Chelysheva et al.,J.Cell.Sci.,2005,118,4621-32)，并且它的抑制改变染色单体的分离。Atspo11-1突变体经历不平衡的第一次分裂，随后进行第二次分裂，产生不平衡的孢子并且是不育的。Atspo11-1/Atrec8双突变体经历有丝分裂样分裂(而不经历正常的第一次减数分裂)，随后进行不平衡的第二次分裂，产生不平衡的孢子并且是不育的(Chelysheva et al.,J.Cell.Sci.,2005,118,4621-32)。
在osd1/spo11-1/rec8三重突变体中，spo11-1和rec8突变的存在导致有丝分裂样的第一次减数分裂，并且osd1突变的存在阻止发生第二次减数分裂。因此减数分裂完全替换成有丝分裂，且不会影响随后的有性生殖过程。因此，将osd1/spo11-1/rec8突变体命名为MiMe(有丝分裂而不是减数分裂)(d’Erfuth et al.,PLoS Biol.,2009,7,e1000124和WO2010/079432)。由MiMe突变体获得的孢子和配子在遗传上与原始细胞相同。
迄今为止，能够产生2n配子或未减数分裂配子的植物的工程因此限于有限数目的基因。
因此，为了增加可以经修饰以产生高频的2n配子或未减数分裂配子的基因的数目，需要参与植物中的这些配子形成的其它基因。
TDM(三次分裂突变，THREE-DIVISION MUTANT)基因，也被命名为TDM1、MS5(蛋白雄性不育，PROTEIN MALE STERILE 5)或POLLENLESS 3编码属于植物中保守的小蛋白家族的蛋白。拟南芥(Arabidopsis thaliana)的TDM基因的序列能够在TAIR数据库中以登录号At4g20900来获得，或在GenBank数据库中以登录号NC_003075.7来获得。它编码434个氨基酸(aa)的蛋白，其序列在所附的序列表中表示为SEQ ID NO：1。
TDM基因被描述为在减数分裂结束时退出减数第二次分裂所需的基因。TDM突变导致多分体(polyad)的形成，以及由于在正常的减数第一次分裂和减数第二次分裂后进入异常的第三次减数分裂而引起的雄性不育。据表明，tdm突变体携带突变，该突变产生编码在其C末端缺少305或112个氨基酸的截短的TDM蛋白的基因(Bulankova et al.,The PlantCell,2010,22,3791-3803；Cromer et al.,PLoS Genet.,2012,8,e10028652012；Gloveret al.,The Plant Journal,1998,15,345-356；Ross et al.,Chrom.Res.,1997,5,551-559；WO/9730581)。
与此相比，如本文所示，本发明人已经确定了TDM基因中的显性突变，该显性突变使得在减数第二次分裂之前提前退出减数分裂，从而产生具有重组遗传信息的二倍体雄SDR配子和二倍体雌SDR配子以及二倍体孢子。此外，他们已经表明，在spo11-1 rec8双突变体的TDM基因中引入显性突变产生了MiMe突变体。
本发明人因此鉴定出了在植物中参与2n配子和未减数分裂配子形成的另一个基因。
与参与二倍体配子产生的其它突变相比(这些突变是隐性的，因此需要初生突变体(突变杂合)的额外自花授粉步骤来获得突变纯合的植物)，TDM中的显性的突变不需要这一步骤。携带TDM基因中的显性突变的初生突变体能够产生2n配子。
发明内容
因此，本发明提供了一种用于获得产生第二次分裂恢复(SDR)2n配子的植物的方法，
其中，所述方法包括提供基因内包括显性突变的植物，所述基因在此指TDM基因，编码在此被指定为TDM蛋白的蛋白；
其中，当所述植物为芸苔属Brassica spp时，所述蛋白与SEQ ID NO:1的TDM蛋白的第1至286位氨基酸残基具有至少75％序列一致性；或者，当所述植物不同于芸苔属Brassica spp时，所述蛋白与所述残基具有至少30％序列一致性，并且所述蛋白的前60个氨基酸包括在此被指定为TPP/Q模体的模体X1X2X3，其中，X1为苏氨酸(T)，X2为脯氨酸(P)，以及X3为脯氨酸(P)或谷氨酰胺(Q)；以及
其中，所述显性突变使所述植物具有产生SDR 2n配子的能力。
在下面的描述中，使用标准的单字母氨基酸代码。
TDM基因和蛋白质序列能够在公共数据库中获得，诸如但不限于，Plaza数据库(http://bioinformatics.psb.ugent.be/plaza/)和phytozome网络门户http://www.phytozome.net/(phytosome v9.1)。
在使用具有默认参数的T-Coffee(v6.85)(http://toolkit.tuebingen.mpg.de/t_coffee)进行序列比对之后，对SEQ ID NO：1的第1至286位残基计算TDM蛋白的蛋白序列一致性；使用Bioedit 7.2.5由该比对获得百分比一致性(Hall,T.A.,1999.BioEdit:auser-friendly biological sequence alignment editor and analysis program forWindows 95/98/NT.Nucl.Acids.Symp.Ser.41:95-98)。
每个物种具有一个或两个TDM基因，通常具有一个(图1和图2，以及表I)。取决于物种，TDM蛋白由约400个氨基酸至约850个氨基酸组成(图1)。根据图1中所示出各种被子植物物种的TDM蛋白的比对表明，TDM蛋白的前半部分是保守的。特别地，所有TDM蛋白的前60个氨基酸都包括保守的TPP/Q模体(图1)。
在使用具有默认参数的T-Coffee(v6.85)(http://toolkit.tuebingen.mpg.de/t_coffee)进行多重序列比对之后，计算各种被子物种的TDM蛋白与SEQ ID NO：1的TDM蛋白的第1至286位残基的百分比序列一致性。使用Bioedit 7.2.5由该比对获得一致性矩阵(Hall，TA，1999.BioEdit：a user-friendly biological sequence alignment editorand analysis program for Windows 95/98/NT.Nucl.Acids.Symp.Ser.41：95-98)。结果示于下表I中。
表I：TDM蛋白的序列一致性
*除了黄瓜Cucumus sativus、番茄Solanum lycopersicum、蓝花耧斗菜Aquilegiacaerulea、菜豆Phaseolus vulgaris、柿树Prunus persica、雷蒙德氏棉Gossypiumraimondii、大豆Glycine max和可可树Theobroma cacao来自于http://www.phytozome.net/(phytosome v9.1)之外，所有的序列都来自于Plaza数据库(http://bioinformatics.psb.ugent.be/plaza/)。
与SEQ ID NO：1的TDM蛋白的第1至286位氨基酸残基具有至少30％序列一致性的序列与SEQ ID NO：1的TDM蛋白的第1至286位氨基酸残基具有至少50％的序列相似性。因此，除了芸苔属Brassica spp.植物以外的其它植物的TDM蛋白可替代地被限定为与SEQ IDNO：1的TDM蛋白的第1至286位氨基酸残基具有至少50％的序列相似性，并且包括在该蛋白的前60个氨基酸中的TPP/Q模体。
根据本发明产生的SDR 2n配子可用于2n配子的所有常见应用中，例如用于生产多倍体植物，或允许不同倍性水平的植物之间进行杂交。它们也可用于遗传作图的方法，例如WO 2006/094774中所公开的“反向子代作图”的方法中。
根据用于获得产生第二次分裂恢复2n配子的植物的方法的优选实施方式，当所述植物不同于芸苔属Brassica spp时，所述蛋白与SEQ ID NO:1的TDM蛋白具有至少35％的序列一致性，并且逐渐优选地，具有至少40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性；或者，当所述植物为芸苔属Brassica spp时，所述蛋白与SEQ ID NO:1的TDM蛋白具有至少80％的序列一致性，并且逐渐优选地，具有至少85％、90％、95％或98％的序列一致性。根据用于获得产生第二次分裂恢复2n配子的植物的方法的另一优选实施方式，所述TPP/Q模体位于所述蛋白的SEQ ID NO：1的第16～18位的区域中。
根据本发明，所述显性突变在TDM基因或TDM转基因的等位基因内，其中，所述TDM基因或转基因来自任何植物物种，例如表I中提及的那些物种。因此，能够产生SDR 2n配子的植物能够通过TDM基因的定向诱变或随机诱变来获得，或者通过遗传转化来获得。
根据本发明的用于获得产生第二次分裂恢复2n配子的植物的方法的优选实施方式，所述方法包括：
提供在TDM基因的等位基因内具有所述显性突变的植物，所述植物对于该突变是杂合的或纯合的。
TDM基因的诱变可以是定向的或随机的。在随机诱变(例如通过EMS诱变)之后，筛选期望基因内的突变体。用于高通量诱变和筛选的方法是本领域可知晓的。作为实例，可以提及TILLING(定向诱导基因组局部突变(Targeting Induced Local Lesions INGenomes)，由McCallum et al.,Plant Physiology,2000,123,439-442所述)。利用标准技术进行定向诱变，该标准技术是本领域所已知的并且利用同源重组，优选与核酸酶例如TALEN或CRISPR组合。
根据本发明的用于获得产生第二次分裂恢复2n配子的植物的方法的另一优选实施方式，所述植物是转基因植物，并且所述方法包括：
a)用包含DNA构建体的载体转化至少一个植物细胞，所述DNA构建体包括具有所述显性突变的TDM基因；
b)培养所述转化的植物细胞，以使在其基因组中具有转基因的植物再生，该转基因包括所述DNA构建体。
DNA构建体包含TDM基因，该TDM基因可来自与其将引入的植物相同的物种，或来自不同的物种。
在TDM基因内的突变中，可以基于对于该突变是杂合的植物的表型性状，来确定导致产生SDR 2n配子的能力的那些突变：这些植物可以形成至少5％，优选至少10％，更优选至少20％，还更优选至少50％，并且高达100％的双孢体，作为减数分裂的产物。
可替代地，可通过它们恢复拟南芥(A.thaliana)spo11/rec8双突变体的可育力的能力，来确定TDM基因内，导致突变体或转基因植物具有产生SDR 2n配子的能力的显性突变，其中可育的spo11/rec8/tdm三重突变体对于TDM突变是杂合的，如本申请的实施例中所证实的，拟南芥(A.thaliana)spo11/rec8双突变体用于筛选显性突变，随后将该显性突变引入到感兴趣的植物中。
根据本发明的用于获得产生第二次分裂恢复2n配子的植物的方法的另一优选实施方式，所述显性突变包括保守TPP/Q模体的至少一个残基的突变或由保守TPP/Q模体的至少一个残基的突变组成。
上述突变可以是置换、插入或缺失，优选置换或缺失。
在更优选的实施方式中，所述显性突变包括T残基的突变和/或它相邻的P残基的突变，或由T残基的突变和/或它相邻的P残基的突变组成。TP是潜在的磷酸化位点。本申请的实施例证实了T16或P17残基的突变能够显著地提前退出减数分裂。不希望受理论的束缚，本发明人认为鉴于这些结果，TDM通过磷酸化来调节，以确保减数第一次分裂至减数第二次分裂的过渡。
因此，突变有利地是消除所述模体的T残基磷酸化的突变，即，破坏TP磷酸化位点的突变。
所述突变有利地是所述T残基和/或P残基被不同的残基置换，例如T被A置换，P被L置换。可替代地，所述突变是缺失T残基和P残基，并且最终缺失所述T残基和/或P残基两侧的额外残基，例如缺失1个至10个，优选1个至5个，甚至更优选1个或2个残基。
如上所限定，本发明的另一方面涉及包括TDM基因的DNA构建体，所述TDM基因具有使突变体/转基因植物具有产生SDR 2n配子的能力的显性突变。TDM基因可来自与其将引入的植物相同的物种，或来自不同的物种。DNA构建体包括可表达形式的TDM基因。优选地，TDM基因位于启动子的转录控制下，该启动子在植物细胞中有功能。启动子可以是TDM基因启动子，诸如所述TDM基因的内源启动子，或在植物中有功能的另一启动子。
在本领域中，具有大量供选择的适于在植物中表达异源基因的启动子。
它们可以例如获自植物、植物病毒或细菌(诸如农杆菌(Agrobacterium))。它们包括：组成型启动子，即在大多数组织和细胞中以及在大多数环境条件下具有活性的启动子；以及，组织特异性启动子或细胞特异性启动子，仅仅或主要在特定组织或特定细胞类型中具有活性；和，诱导型启动子，该诱导型启动子通过物理刺激或化学刺激，诸如由于线虫感染而产生的物理刺激或化学刺激而被激活。对启动子进行选择以便在性母细胞中具有功能。
常用于植物细胞中的组成型启动子的非限制性实例是花椰菜花叶病毒(CaMV)35S启动子、Nos启动子、1,5-二磷酸核酮糖羧化酶/加氧酶(rubisco)启动子、木薯叶脉花叶病毒(CsVMV)启动子。
可用于本发明的器官或组织特异性启动子特别包括能够赋予减数分裂相关表达的启动子，诸如DMC1启动子(Klimyuk&Jones，Plant J，1997,11,1-14)。
本发明的DNA构建体通常还包括转录终止子(例如，35S转录终止子、胭脂碱合酶(Nos)转录终止子或TDM基因终止子)。
本发明还包括重组载体，所述重组载体包含本发明的DNA构建体。通常，所述重组载体还包括一个或多个标记基因，允许对转化的宿主进行选择。
合适载体的选择，以及用于将DNA构建体插入到其中的方法是本领域普通技术人员所熟知的。载体的选择取决于预期的宿主以及所述宿主的预期的转化方法。在本领域中，对于许多植物物种，双子叶植物或单子叶植物，有多种用于植物细胞或植物的遗传转化方法。作为非限制性实例，可提及的是：病毒介导的转化，通过显微注射、电穿孔进行的转化，微粒介导的转化，农杆菌(Agrobacterium)介导的转化等。
本发明还提供了包括本发明的重组DNA构建体的宿主细胞。所述宿主细胞可以是：原核细胞，例如农杆菌(Agrobacterium)细胞；或者真核细胞，例如通过本发明的DNA构建体遗传转化的植物细胞。构建体可被瞬时表达；它也可被并入到稳定的染色体外复制子中，或整合到染色体中。
本发明人还发现，通过使TDM突变中的显性突变与两个基因的失活相组合，来得到产生未减数分裂配子的MiMe突变体，该两个基因中的一个基因对于减数分裂重组的起始是必需的并且选自spo11-1、SPO11-2、PRD1、PRD2(AT5G57880)、PRD3/PAIR1和DFO(AT1G07060)，另一个基因为REC8。
由MiMe突变体产生的未减数分裂配子可以与SDR 2n配子相同的方式，用于生产多倍体植物或用于不同倍性水平的植物的杂交。它们对于生产无融合生殖植物也是感兴趣的，即能够从胚珠的母体组织形成种子的植物，从而产生为母本的遗传克隆的子代。
因此，本发明的另一目的是用于获得产生未减数分裂配子的植物的方法，其中所述方法包括：
a)提供植物，所述植物包括如上定义的在TDM基因中的显性突变；
b)在所述植物中，抑制参与植物中起始减数分裂重组的第一蛋白，所述蛋白选自以下蛋白：
被指定为SPO11-1蛋白的蛋白，其中，所述蛋白与SPO11-1蛋白具有至少40％的序列一致性，并且逐渐优选地，具有至少45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白与SPO11-1蛋白具有至少60％的序列相似性，并且逐渐优选地，具有至少65％、70％、75％、80％、85％、90％、95％或98％的序列相似性，所述SPO11-1蛋白在SwissProt数据库中的序列登录号为Q9M4A2，对应于所附序列表中的SEQ ID NO:29；
被指定为SPO11-2蛋白的蛋白，其中，所述蛋白与SPO11-2蛋白具有至少40％的序列一致性，并且逐渐优选地，具有至少45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白与SPO11-2蛋白具有至少60％的序列相似性，并且逐渐优选地，具有至少65％、70％、75％、80％、85％、90％、95％或98％的序列相似性，所述SPO11-2蛋白在SwissProt数据库中的序列登录号为Q9M4A2，对应于所附序列表中的SEQ ID NO:30；
被指定为PRD1蛋白的蛋白，其中，所述蛋白与PRD1蛋白具有至少25％的序列一致性，并且逐渐优选地，具有至少30％、35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白与PRD1蛋白具有至少35％的序列相似性，并且逐渐优选地，具有至少40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列相似性，所述PRD1蛋白在GenBank数据库中的序列登录号为ABQ12642，对应于所附序列表中的SEQ ID NO:31；
被指定为PRD2蛋白的蛋白，其中，所述蛋白与PRD2蛋白具有至少25％的序列一致性，并且逐渐优选地，具有至少30％、35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白与PRD2蛋白具有至少35％的序列相似性，并且逐渐优选地，具有至少40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列相似性，所述PRD2蛋白在Plaza数据库中的序列登录号为AT5G57880，对应于所附序列表中的SEQ ID NO:32；
被指定为PAIR1蛋白的蛋白，其中，所述蛋白与PAIR1蛋白具有至少30％的序列一致性，并且逐渐优选地，具有至少35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白与PAIR1蛋白具有至少40％的序列相似性，并且逐渐优选地，具有至少45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列相似性，所述PAIR1蛋白在GenBank数据库中的序列登录号为NP_171675，对应于所附序列表中的SEQ ID NO:33；
被指定为DFO蛋白的蛋白，其中，所述蛋白与DFO蛋白具有至少30％的序列一致性，并且逐渐优选地，具有至少35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白与DFO蛋白具有至少40％的序列相似性，并且逐渐优选地，具有至少45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列相似性，所述DFO蛋白在Plaza数据库中的序列登录号为AT1G07060，对应于所附序列表中的SEQ ID NO:34；以及
c)在所述植物中，抑制被指定为REC8蛋白的第二蛋白，其中，所述蛋白与REC8蛋白具有至少40％的序列一致性，并且逐渐优选地，具有至少45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白与REC8蛋白具有至少45％的序列相似性，并且逐渐优选地，具有至少50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列相似性，所述REC8蛋白在GenBank数据库中的序列登录号为NP_196168，对应于所附序列表中的SEQ ID NO:35。
使用在默认参数下的BLASTP程序，计算spo11-1、spo11-2、prd1、PRD2、DFO、PAIR1或REC8蛋白的在此所提供的蛋白序列一致性和相似性的值。使用评分矩阵BLOSUM62，进行相似性计算。
可通过消除、阻断或降低其功能，或者有利地通过阻止或下调相应基因的表达，来获得对上述SPO11-1、SPO11-2、PRD1、PRD2、PAIR1、DFO或Rec8蛋白的抑制。在申请WO2010/00431中详细公开了SPO11-1、SPO11-2、PRD1、PRD2、PAIR1、DFO或Rec8蛋白以及所述蛋白的抑制。
例如，所述蛋白的抑制可以通过诱变相应的基因或其启动子，并选择所述蛋白的活性部分或完全丧失的突变体来获得。所述抑制公开在WO 2010/00431的第5页的最后一段开始至第6页的第4段结束，以及第6页的最后一段，这些内容通过引用并入本文中。
可替代地，通过使相应的基因沉默来获得目标蛋白的抑制。这种抑制公开在WO2010/00431的第7页第4段开始至第9页的倒数第二段结束，以及第10页的前三段，这些内容通过引用并入本文中。
根据本发明的用于获得能够产生未减数分裂配子的植物的方法的优选实施方式，所述方法包括：
a)提供在TDM基因的等位基因内具有显性突变，从而具有产生SDR 2n配子的能力的植物，该植物对于该突变是杂合的；
b)提供在选自SPO11-1、SPO11-2、PRD1、PRD2、DFO或PAIR1基因的基因的等位基因内具有突变，从而抑制由所述等位基因编码的蛋白的植物，所述植物对于该突变是杂合的；
c)提供在REC8基因的等位基因内具有突变，从而抑制由所述等位基因编码的蛋白的植物，所述植物对于该突变是杂合的；
e)使步骤a)、步骤b)和步骤c)的植物杂交，以获得在TDM基因的等位基因内具有显性突变，在选自SPO11-1、SPO11-2、PRD1、PRD2、DFO或PAIR1基因的基因的等位基因内具有突变，以及在REC8基因的等位基因内具有突变的植物，所述植物对于每一突变都是杂合的；
f)使步骤e)的植物自花授粉，以获得对于TDM基因内的突变是纯合的，对于选自SPO11-1、SPO11-2、PRD1、PRD2、DFO或PAIR1基因的基因内的突变是纯合的，以及对于REC8基因内的突变的是纯合的植物。
根据本发明的用于获得能够产生未减数分裂配子的植物的方法的另一优选实施方式，所述植物是转基因植物，并且所述方法包括：
a)用含有本发明的包含如上定义的TDM基因中的显性突变的DNA构建体的载体，含有靶向选自SPO11-1、SPO11-2、PRD1、PRD2、DFO或PAIR1的基因的DNA构建体的载体，以及含有靶向REC8基因的DNA构建体的载体转化至少一个植物细胞；
b)培养所述转化的植物细胞，以使在它的基因组中具有含所述DNA构建体的转基因的植物再生。
在TDM基因中包括显性突变的DNA构建体的表达，为所述转基因植物提供了产生2nSDR配子的能力。包括TDM基因中的显性突变的DNA构建体基因，靶向选自SPO11-1、SPO11-2、PRD1、PRD2、DFO或PAIR1的基因的DNA构建体，以及靶向REC8基因的DNA构建体的共表达，使由这三种基因编码的蛋白下调，并且为所述转基因植物提供产生未减数分裂配子的能力。
本发明还涵盖能够通过本发明的方法获得的能够产生SDR 2n配子或未减数分裂配子的植物。
这特别包括如上定义的在TDM基因内包含显性突变的植物，以及进一步包括第一突变和第二突变的植物，所述第一突变在选自SPO11-1、SPO11-2、PRD1、PRD2、DFO或PAIR1基因的基因内，其中由所述基因编码的SPO11-1、SPO11-2、PRD1、PRD2、DFO或PAIR1蛋白因该突变而被抑制，所述第二突变在REC8基因内，其中REC8蛋白因该突变而被抑制。
这还包括由本发明的至少一种DNA构建体遗传转化的植物。优选地，所述植物是转基因植物，其中所述构建体被包含在整合到植物基因组中的转基因中，从而使它被传递给连续的植物后代中。
本发明还涵盖用于产生SDR 2n配子的方法，其中所述方法包括培养能够通过本发明的方法获得的植物并回收由所述植物产生的配子。优选地，所述配子包括至少10％、更优选至少20％，并且逐渐优选地，至少30％、40％、50％、60％、70％、80％或90％的可育2n配子。
本发明还还涵盖用于产生未减数分裂配子的方法，其中所述方法包括培养能够通过本发明的方法获得的植物并回收由所述植物产生的配子。优选地，所述配子包括至少10％、更优选至少20％，并且逐渐优选地，至少30％、40％、50％、60％、70％、80％或90％的可育未减数分裂配子。
本发明适用于农艺学上感兴趣的范围广泛的单子叶植物或双子叶植物。作为非限制性实例，可提及的是土豆、稻、小麦、玉米、番茄、黄瓜、苜蓿、甘蔗、甘薯、木薯、三叶草、大豆、射线草、香蕉、甜瓜、西瓜、棉，或观赏植物(诸如玫瑰、百合、郁金香和水仙)。
除非另有说明，否则本发明的实施将采用本领域技术范围内的常规技术。这样的技术在文献中有充分的解释。
附图说明
除了上述设置之外，本发明还包括其他设置，这些其他设置，将在下文中的描述中显现出来，并且参照所附附图，指本发明主题的示例性实施方式，在附图中：
图1示出了来自多种被子植物物种的TDM蛋白的比对。使用具有默认参数的T-Coffee(v6.85)对序列进行比对(http://toolkit.tuebingen.mpg.de/t_coffee)。序列比对用BioEdit编辑。仅示出了在TDM蛋白中保守的序列的前半部分。在比对的TDM蛋白中显示出80％以上一致性的残基用阴影表示。包括TPP/Q模体的保守区则加边框来表示。
图2示出了来自多种被子植物的TDM蛋白、来自十字花目的TDM_样1蛋白以及来自拟南芥(Arabidopsis thaliana)和二穗短柄草(Brachypodium distachyon)的TDM样蛋白的系统树。该分析在Phylogeny.fr平台上进行并包括以下步骤。使用以下成对比对方法，利用T-Coffee(v6.85)来进行序列比对：10次最佳局部比对(Lalign_pair)，精确的全局比对(slow_pair)。在比对之后，从比对中去除具有缺口的位置。利用在PhyML程序(v3.0aLRT)中实施的最大似然法来重建系统树。示出了所有物种中的TDM进化枝的蛋白(包括在十字花目中的TDM-样1)。仅示出了拟南芥(Arabidopsis thaliana)和二穗短柄草(Brachypodiumdistachyon)的更远的TDM旁系同源。
At：拟南芥Arabidopsis thaliana，A1：深山南芥Arabidopsis lyrata，Bra：芸苔Brassica rapa，Sly：番茄Solanum lycopersicum，St：马铃薯Solanum tuberosum，Csa：黄瓜Cucumis sativus，Eucgr：桉树Eucalyptus grandis，Cp：番木瓜Carica papaya，ME：木薯Manihot esculenta，TC：可可树Theobroma cacao，Goraii：雷蒙德氏棉Gossypiumraimondii，FV：野草莓Fragaria vesca，Pp：柿树Prunus persica，LJ：莲藕Lotusjaponicus，MT：蒺藜苜蓿Medicago truncatula，GM：大豆Glycine max，Pv：芸豆Phaseolusvulgaris，VV：菜豆Phaseolus vulgaris，Aq：蓝花耧斗菜Aquilegia caerula，OS：粳稻Oryza sativa japonica，OSINDICA：籼稻Oryza sativa indica，BD二穗短柄草Brachypodium distachyon，SB：高粱Sorghum bicolor，ZM：玉米Zea mays，Si：粟Setariaitalica。
图3显示spo11-1 rec8(s)-40突变体产生二分体并且是四倍体的。(A～C)。用甲苯胺蓝染色的雄性减数分裂产物。(A)野生型产生孢子的四分体。(B)spo11-1 rec8产生孢子的不平衡的多分体。(C)spo11-1 rec8-40产生孢子的二分体。(D～F)有丝分裂的核型。(D)野生型是二倍体，具有排列在有丝分裂中期板上的10个染色体。(E)spo11-1 rec8是二倍体。(F)spo11-1 rec8(s)-40是四倍体，具有排列在在有丝分裂中期板上的20个染色体。比例尺＝10μM。
图4示出了TDM-P17L、TDM-T16A和TDM-Δ14-19的减数分裂产物。(A)用TDM-P17L转化的spo11-1 rec8突变体。由(B)TDM-P17L、(C)TDM-T16A或(D)TDM-Δ14-19转化的野生型植物。与野生型中的四分体(图3A)和spo11-1 rec8中的多分体(图3B)相比，观察到孢子的二分体。
图5示出了在野生型、spo11-1 rec8、TDM-P17和spo11-1 rec8 TDM-P17植物中的减数分裂的染色体分散。(A至D)野生型的减数分裂。(A)五个二价体在中期I时对齐，和(B)成对的同源染色在末期I时分布至两个核中。(C)五对姐妹染色单体在两个中期板上对齐。(D)在末期II，形成四个平衡的核。(E至H)spo11-1 rec8中的减数分裂。第一次分裂类似于有丝分裂，其中，(E)10对染色单体对齐在中期板上，并且(F)分离成两组10个染色单体。(G)在中期II，单个染色单体未能适当地对齐，导致(H)末期II时产生可变数目的不平衡的核。(I至J)用TDM-P17转化的野生型植物中的减数分裂。观察到单次减数第一次分裂样的分裂。(K至L)用TDM-P17转化的spo11-1 rec8植物中的减数分裂。观察到单次有丝分裂样的分裂。
具体实施方式
实施例
实验步骤
1.生长条件和基因分型
在21℃下，在16小时至18小时光周期和70％相对湿度下，如先前所述在温室中(Vignard et al.,PLoS Genet.,2007,3,1894-1906)或者如前所述离体在拟南芥Arabidopsis培养基上(Estelle and Somerville,Mol.Genet.,1987,206,200-206)上培养Arabidopsis拟南芥植物。
如先前所述(d’Erfurth et al.,PLoS Biol.,2009,7,e1000124)对spo11-1-3rec8-2植物进行基因分型。如Cromer et al.,PLoS Genet.,2012,8,e1002865所述对tdm-3植物进行基因分型。
2.EMS诱变和突变鉴定
如先前所述(Crismani et al.,Science,2012,336,1588-1590)进行EMS诱变。通过HigSeqTM2000(Illumina)进行全基因组测序。与拟南芥Arabidopsis thaliana TAIR10(哥伦比亚栽培品种)的对照基因组相比，生成SNP列表。
3.细胞学和倍性分析
使用由d'Erfurth等人(PLoS Genet.,2008,4,e1000274)描述的技术，对雄性减数分裂产物进行观察、染色体分散和倍性测量。
4.定向诱变构建体和植物转化
使用TDM U(5'-GACATCGGCACTTGCTTAGAG-3'；SEQ ID NO：36)和TDM L(5'-GCGATATAGCTCCCACTGGTT-3'；SEQ ID NO：37)通过PCR扩增TDM基因组片段。扩增覆盖ATG前的986个核苷酸和终止密码子后的537个核苷酸。通过GatewayTM技术(Invitrogen)，将PCR产物克隆到pDONR207TM载体(Invitrogen)中，以产生pENTR-TDM；使用StratageneQuickChangeTM定点诱变试剂盒，根据制造商的说明对pENTR-TDM进行定向诱变。用于产生TDM的突变形式的诱变引物为SEQ ID NO：38至SEQ ID NO：41：
-TDM-P17L:5′-GAGTTTACTATACTCTGCCGCCGGCGAGAAC-3′；
-TDM-T16A:5′-CTCCACCTGGAGTTTACTATGCCCCGCCGCCGGCGAGA-3′；
-TDM-Y14A:5’-CCACCTGGAGTTGCGTATACTCCGCCGCGGCG-3′；
-TDM-Δ14-19:5’-CCACCTGGAGTTGCGAGAACAAGTGATCATGTGGC-3’；
以及它们各自的反向互补引物。为了产生用于植物转化的双元载体，使用GatewayTM系统的二元载体pGWB1进行LR重组反应(Nakagawa et al.,Journal of Bioscience andBioengineering.2007,104,34-41)。对野生型植物以及分离成spo11-1或rec8或tdm-3突变的植物群，使用农杆菌介导的花浸渍法(Clough,S.J.and Bent A.F.,Plant J.,1998,16,735-743)转化得到的二元载体pTDM，pTDM-P17L，pT16A和pTDM-Y14A。在含有20mg/L潮霉素的琼脂板上对转化的植物进行选择。
实施例1：TDM中的显性突变导致提早退出减数分裂
为了鉴定控制减数分裂进程的新基因，基于以下观点来设计遗传筛选：导致遗漏第二次减数分裂的突变，诸如osd1和cyca1；2/tam将恢复仅在第二次减数分裂中具有不平衡的染色体分离缺陷的突变体的可育力(d’Erfurth et al.,PLoS Biol.,2009,7,e1000124；d’Erfurth et al.,PLoS Genet.,2010,6,e1000989和WO 2010/079432)。这是spo11rec8双突变体的情况，其中第一次减数分裂类似于有丝分裂(姐妹染色单体平衡分离至相对极)，但是第二次分裂是不平衡的且产生非整倍体的配子，因此具有非常有限的可育力(图5)(Chelysheva et al.,J.Cell.Sci.,2005,118,4621-32)。在OSD1(d’Erfurth etal.,PLoS Biol.,2009,7,e1000124 2009)或CYCA1；2/TAM(d’Erfurth et al.,PLoSGenet.,2010,6,e1000989)中的导致在减数第二次分裂前退出减数分裂的突变，确实能够恢复spo11-1 rec8的可育力。因此，基于spo11-1 rec8的可育力的恢复来运行遗传筛选，旨在鉴定出赋予比osd1或tam还相似缺陷的突变体。尽管它们具有减数分裂分离的缺陷，spo11-1 rec8植物产生了经甲基磺酸乙酯(EMS)诱变的足够的剩余种子。假定对EMS突变是杂合的M1植物进行自花授粉，并且在5个批次中收获以产生M2家族。筛选了与spo11-1 rec8非诱变对照相比，可育力增加的约2000个M2家族(400个批次)。
三个批次分离具有增加可育力的植物。基因分型证实，它们是spo11-1 rec8突变体，这表明了其是真正的抑制因子。通过甲苯胺蓝染色对雄性减数分裂产物进行的分析示出，在所有三种情况下，可育植物产生孢子的二分体(如在osd1或cyca1；2/tam中所观察到的)，而不是四分体(如在野生型中所观察到的)，表明了在这些植物中并没有发生第二次减数分裂(图3)。候选基因(CYCA1；2/TAM和OSD1)的测序确定了CYCA1；2/TAM在三个家族中的两个中的隐性突变。所确定的突变是外显子7(TAIR10chr1：29082522C>T)中的剪接位点，和5'UTR区域中的引入上游框外起始密码子(TAIR10chr1：29084174G>A)的突变。互补试验表明它们是等位基因，证实了CYCA1；2中的突变引起二分体表型和可育力的恢复。第三个家族(spo11rec8(s)-40)在OSD1和CYCA1；2中没有突变，是本研究的重点。
出乎意料地，染色体分散表明四种植物是四倍体的(图3)。这表明因果突变(causal mutation)是显性的，并且导致在M1植物的雄性和雌性器官中均产生二倍体配子。与野生型相比，以～100X覆盖度对两种姐妹植物的大部分所进行全基因组测序显示出1144个SNP的存在。然而，仅15个SNP呈现为纯合子。这些少数纯合子SNP分散在基因组中，表明它们在诱变前就存在于spo11-1rec8株系中，而不是由于EMS诱导的突变所固定的。几乎所有检测到的突变都是杂合子这一事实进一步表明因果突变是显性的。该突变会在M1植物中显型表达，在与spo11-1 rec8突变组合下，导致二倍体克隆配子的产生(如在spo11-1rec8osd1三重突变体中观察到的)(MiMe,d’Erfurth et al.,PLoS Biol.,2009,7,e1000124和WO 2010/079432)，因此四倍体M2植物中维持了来自M1植物的EMS诱导的突变的杂合性。
然后，在杂合子SNP中寻找候选的因果突变。在这1129个突变中，预测341个突变将影响编码序列(无义、错义或剪接位点)。其中，TDM中导致氨基酸变化的突变(TDM-P17L)由于潜在的因果显性突变而成为良好候选者。之前已证实，TDM对于减数第二次分裂结束时退出减数分裂是必需的。即使在tdm敲除突变体中观察到的减数分裂缺陷(额外的一轮分裂)与(spo11rec8(s)-40)缺陷显著不同，TDM中的显性突变仍然是(spo11rec8(s)-40)中的因果突变的潜在候选者。
为了验证该假设，产生含有TDM基因(包括启动子和终止子)的基因组克隆，并使其突变以重新产生在筛选中所鉴定的突变(TDM-P17L)，其中TDM基因能够补充tdm-3突变体(n＝8个转化体，100％四分体)。当引入spo11-1rec8植物中时，TDM-P17L克隆恢复了初代转化体的可育力(n＝2/3，spo11-1 rec8：0.1个种子/果实(n＝197)，spo11-1 rec8 TDM-P17L#15：25个种子/果实(n＝15)，spo11-1 rec8 TDM-P17L#67：48个种子/果实(n＝10))，并产生二分体(图4，表II)。这表明TDM中的突变确实是spo11rec8(s)-40中的因果显性突变。对spo11-1rec8TDM-P17L转化体中减数分裂染色体分散的分析显示出，有丝分裂样的第一次分裂，其中10个单价体在中期I时对齐，并且姊妹染色单体在后期I分离，且不进行第二次分裂(图5)。接下来，研究了spo11-1rec8 TDM-P17L后代的倍性水平。在自交子代中，仅发现四倍体(4n)(表III)。当spo11-1rec8 TDM-P17L花粉用于使野生型植物受精时，所有得到的子代都是三倍体(表III)。当spo11-1 rec8 TDM-P17L胚珠经野生型花粉粒受精时，仅发现三倍体植物(表III)。这证实了被TDM-P17L转化的spo11-1 rec8突变体产生高水平的雄性和雌性(100％)有丝分裂样来源的孢子，其导致功能性二倍体配子。
当引入到野生型植物和tdm-3突变体中时，TDM-P17L基因组克隆通过产生二分体来修饰两种基因型的减数分裂表型(图4，表II)。
表II：初代转化体的减数分裂产物
产生二分体的TDM-P17L植物显示出野生型第一次分裂并且不发生减数第二次分裂(图5)，从而形成2n配子，以及来自osd1和cyca1；2/tam之一的表型重现(d’Erfurth etal.,PLoS Biol.,2009,7,e1000124和WO 2010/079432；d’Erfurth et al.,PLoS Genet.,2010,6,e1000989)。在TDM-P17L植物的后代中测量倍性水平(表III)。在自交子代中，发现了四倍体和三倍体。当TDM-P17L胚珠经野生型花粉粒受精时，分离出二倍体植物和三倍体植物(表III)。
表III：spo11-1 rec8 TDM-P17L和TDM-P17L后代的倍性
总之，tdm-p17L显性突变赋予了与隐性osd1或tam突变类似的减数分裂缺陷，使在第二次分裂之前提前退出减数分裂，从而产生二倍体的雄性和雌性配子。
TDM属于植物中保守的小家族蛋白。例如，拟南芥Arabidopsis基因组含有与TDM显示出显著序列相似性的5个其他基因(图2)。这些类TDM基因的功能是未知的。蛋白序列的分析表明，因果突变在仅TDM蛋白亚家族中保守的小结构域中，所述TDM蛋白亚家族通常每个植物物种含有一个或两个基因(图1)。Pro17氨基酸以及相邻的Thr16氨基酸是绝对保守的(图1)。这就限定了T16上的最小共有磷酸化位点。为了对此进行验证，通过用不可磷酸化的氨基酸(TDM-T16A)来置换可磷酸化的氨基酸，并通过删除整个保守结构域(TDM-Δ14_19)来建立基因组TDM基因的两个其它潜在的磷酸化丢失形式。当引入到野生型植物中时，TDM-T16A和TDM-Δ14_19都以显性方式产生了二分体表型，重现了TDM-P17L的作用(表II；图4)。此外，当引入到tdm-3突变体时，TDM-Δ14_19也显示二分体表型(表II)。然而，当引入到野生型时，TDM酪氨酸14(TDM-Y14A)(结构域的保守性稍低的氨基酸)的突变不能产生二分体表型，但能够补充tdm-3突变(表II)。总之，TDM-P17L、TDM-T16A和TDM-Δ14-19突变的表达同样能够显性地提早退出减数分裂。由于TP是潜在的磷酸化位点，这个结果表明TDM可能受到磷酸化的调节以确保减数第一次分裂至减数第二次分裂的过渡。
序列表
<110> 国家农艺研究所
<120> 在植物中产生二倍体配子的TDM基因中的显性突变
<130> 539EP166
<160> 41
<170> PatentIn 3.5版
<210> 1
<211> 434
<212> PRT
<213> 拟南芥（Arabidopsis thaliana）
<400> 1
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Lys Ser Glu Ser Lys Lys Ser Trp Ala Asp Ile Ala Glu Glu Glu Glu
405 410 415
Ala Glu Glu Glu Glu Glu Glu Arg Leu Gln Gly Glu Leu Lys Thr Ala
420 425 430
Glu Met
<210> 2
<211> 426
<212> PRT
<213> 深山南芥（Arabidopsis lyrata）
<400> 2
Met Cys Pro Cys Glu Glu Arg Arg Ala Pro Pro Gly Val Tyr Tyr Thr
1 5 10 15
Pro Pro Pro Ala Arg Thr Ser Tyr Val Ala Ala Met Pro Leu Ser Glu
20 25 30
Arg Arg Arg Pro Pro Phe Ser Cys Ser Ser Ser Ser Glu Lys Arg Asp
35 40 45
Pro Phe His Ile Val His Lys Val Pro Ser Gly Asp Ser Pro Tyr Val
50 55 60
Arg Ala Lys His Ala Gln Leu Ile Asp Lys Asp Pro Asn Arg Ala Ile
65 70 75 80
Ser Leu Phe Trp Thr Ala Ile Asn Ala Gly Asp Arg Val Asp Ser Ala
85 90 95
Leu Lys Asp Met Val Val Val Met Lys Gln Leu Asp Arg Ser Asp Glu
100 105 110
Gly Ile Glu Ala Ile Arg Ser Phe Arg Tyr Leu Cys Ser Phe Glu Ser
115 120 125
Gln Asp Ser Ile Asp Asn Leu Leu Leu Glu Leu Tyr Lys Lys Ser Gly
130 135 140
Arg Ile Glu Glu Glu Ala Glu Leu Leu Glu His Lys Leu Lys Thr Leu
145 150 155 160
Glu Gln Gly Met Gly Phe Gly Gly Arg Val Ile Arg Ala Lys Arg Val
165 170 175
Gln Gly Lys His Val Thr Met Thr Ile Glu Gln Glu Lys Ala Arg Val
180 185 190
Leu Gly Asn Leu Gly Trp Val His Leu Gln Leu His Asn Tyr Gly Ile
195 200 205
Ala Glu Gln His Tyr Arg Arg Ala Leu Gly Leu Glu Arg Asp Lys Asn
210 215 220
Lys Gln Cys Asn Leu Ala Ile Cys Leu Met Arg Met Gly Arg Ile Pro
225 230 235 240
Glu Ala Lys Ser Leu Leu Asp Asp Val Arg Asp Ser Pro Thr Glu Ser
245 250 255
Glu Cys Gly Asp Glu Pro Phe Ala Lys Ser Tyr Asp Arg Ala Val Glu
260 265 270
Met Leu Ala Glu Ile Glu Ser Lys Asn Pro Glu Ala Asp Leu Ser Asp
275 280 285
Lys Phe Tyr Ala Gly Cys Ser Phe Ala Asn Gly Met Lys Glu Asn Ile
290 295 300
Ala Pro Gly Ile Ala Asn Lys Asn Tyr Ser His Val Ser Ser Ser Pro
305 310 315 320
Ala Ser Val Val Pro Asn Ser Ala Gly Leu Tyr Thr Gln Pro Arg Gly
325 330 335
Cys Arg Ala Gly Met Tyr Glu Glu Glu Thr Arg Gly Ala Ala Arg Lys
340 345 350
Leu Leu Phe Glu Lys Pro Lys Pro Phe Ala Ser Glu Gln Ile Lys Ile
355 360 365
Leu Lys Arg Gly Glu Glu Glu Pro Gln Lys Arg Lys Lys Leu Asp Pro
370 375 380
Asn Met Ile Gln Tyr Leu His Glu Phe Ile Lys Asp Thr Ala Asp Gly
385 390 395 400
Pro Lys Asn Glu Ser Lys Lys Ser Trp Ala Asp Ile Ala Glu Glu Glu
405 410 415
Glu Glu Glu Glu Arg Leu Gln Ala Glu Thr
420 425
<210> 3
<211> 432
<212> PRT
<213> 芸苔（Brassica rapa）
<400> 3
Met Cys Pro Phe Glu Asp Arg Arg Ala Pro Pro Gly Val Tyr Trp Thr
1 5 10 15
Pro Pro Pro Ala Arg Arg Thr Glu Asn Ala Ala Ala Met Pro Met Pro
20 25 30
Leu Ser Glu Arg Arg Arg Pro Pro Ser Ser Glu Lys Arg Asp Pro Phe
35 40 45
His Ile Val His Lys Val Pro Ser Gly Asp Ser Pro Tyr Val Arg Ala
50 55 60
Lys His Ala Gln Leu Val Tyr Lys Asp Pro Asn Arg Ala Ile Ser Leu
65 70 75 80
Phe Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Asp Ser Ala Leu Lys
85 90 95
Asp Met Ala Val Val Phe Lys Lys Leu Asp Arg Ser Asp Glu Gly Ile
100 105 110
Glu Ala Ile Lys Ser Phe Arg Tyr Leu Cys Pro Phe Glu Ala Gln Asp
115 120 125
Ser Ile Asp Asn Leu Leu Leu Glu Leu Tyr Lys Lys Ser Gly Arg Ile
130 135 140
Gln Glu Glu Ala Glu Leu Leu Glu His Lys Leu Lys Val Ile Glu Gln
145 150 155 160
Gly Met Gly Phe Gly Gly Arg Ile Val Arg Ala Lys Arg Val Gln Gly
165 170 175
Lys His Val Thr Met Thr Val Glu Gln Glu Lys Ala Arg Val Leu Gly
180 185 190
Asn Leu Gly Trp Val His Leu Gln Leu His Asn Tyr Gly Ile Ala Glu
195 200 205
Gln His Tyr Arg Arg Ala Leu Cys Leu Glu Pro Asp Lys Asn Lys Gln
210 215 220
Cys Asn Leu Ala Ile Cys Leu Met Arg Met Gly Arg Ile Pro Glu Ala
225 230 235 240
Lys Ser Met Ile Asp Ala Val Arg Asp Ser Ser Ala Glu Thr Glu Ser
245 250 255
Gly Asp Glu Pro Phe Thr Lys Ser Tyr Asn Arg Ala Val Glu Met Leu
260 265 270
Ala Glu Val Glu Ser Lys Asp Pro Glu Asp Gly Leu Ser Asp Lys Phe
275 280 285
Tyr Ala Gly Cys Ser Phe Ala Asn Gly Thr Met Lys Glu Asn Lys Ala
290 295 300
Pro Arg Asn Ala Asn Arg Asn His Ser His Val Pro Pro Ser Pro Ala
305 310 315 320
Ser Val Arg Gln Asn Ser Ala Gly Leu Phe Thr Gln Pro Arg Gly Cys
325 330 335
Lys Gly Asp Pro Lys Asn Gly Val Ser Glu Glu Glu Thr Gly Gly Ala
340 345 350
Ala Arg Lys Leu Leu Phe Asp Lys Pro Ile Gly Ser Gln Arg Val Lys
355 360 365
Leu Leu Lys Ser Gly Glu Gly Glu Gln His Val Lys Gly Lys Lys Leu
370 375 380
Asp Gln Asn Met Ile Gln Asp Leu His Glu Tyr Ile Lys Asp Thr Ala
385 390 395 400
Asp Cys Leu Lys Ser Gly Ser Lys Lys Ser Trp Ala Asp Met Ala Glu
405 410 415
Glu Glu Asp Glu Glu Ser Val Gln Ser Gln Leu Lys Thr Ala Lys Ile
420 425 430
<210> 4
<211> 670
<212> PRT
<213> 番木瓜（Carica papaya）
<400> 4
Met Trp Ala Asn Asp Arg Lys Pro Leu Gly Arg Gly Phe Ser Thr Pro
1 5 10 15
Gln Pro Thr Trp Asn Ser Arg Pro Arg Asp Ser Leu Val Met Pro Met
20 25 30
Ser Glu Arg Lys Ser Ser Ser Pro Val His Lys Gly Asp Leu Phe His
35 40 45
Val Val His Lys Val Pro Ala Gly Asp Ser Pro Tyr Val Arg Ala Lys
50 55 60
His Val Gln Leu Ile Glu Lys Asp Pro Ser Arg Ala Ile Ser Leu Phe
65 70 75 80
Trp Ala Ala Ile Asn Ala Gly Asp Arg Ile Asp Ser Ala Leu Lys Asp
85 90 95
Met Ala Val Val Met Lys Gln Leu Asn Arg Ser Asp Glu Ala Ile Glu
100 105 110
Ala Ile Lys Ser Phe Arg His Leu Cys Pro Tyr Asp Ala Gln Glu Ser
115 120 125
Leu Asp Asn Val Leu Val Glu Leu Tyr Lys Arg Ser Gly Arg Ile Glu
130 135 140
Glu Glu Val Glu Leu Leu Gln His Lys Leu Lys Asn Ile Glu Gly Val
145 150 155 160
Thr Phe Gly Gly Lys Arg Thr Lys Thr Ala Arg Ser Gln Gly Lys Lys
165 170 175
Ile Gln Ile Thr Val Glu Gln Glu Lys Ser Arg Ile Leu Gly Asn Leu
180 185 190
Ala Trp Ala Tyr Leu Gln Gln His Asn Tyr Gly Ser Ala Glu Gln Tyr
195 200 205
Tyr Arg Lys Ala Leu Ser Leu Glu Val Asp Lys Asn Lys Gln Cys Asn
210 215 220
Leu Ala Ile Cys Leu Met His Met Asn Arg Ile Ala Glu Ala Lys Ser
225 230 235 240
Leu Leu Leu Ala Val Arg Asp Ser Pro Lys Lys Arg Gln Met Asp Glu
245 250 255
Ser Cys Ala Lys Ser Phe Glu Arg Ala Tyr Gln Ile Leu Arg Glu Lys
260 265 270
Glu Ser Gln His Glu Arg Ser Gly Arg Ser Glu Asp Ser Phe Tyr Val
275 280 285
Val Asp Asn Glu Ser Ser Asn Ser Asp Lys Asn Gly Leu Asp Asn Trp
290 295 300
Ser Asn Gly Cys Cys Ser Glu Ser Ser Leu Glu Met Ser Asn Phe Ala
305 310 315 320
Asp Gly Met Lys Arg Tyr Gln Asn Gly Lys Glu Thr Glu Ala Ser Leu
325 330 335
Gly Asn Asn Ser Tyr Ser Thr Cys Thr Arg Arg Lys Ser Gly Ile Phe
340 345 350
Leu Thr Gln Pro Arg Arg Cys Ser Trp Gly Leu Glu Asp Glu Asn Tyr
355 360 365
Arg Glu Ile Trp Gly Lys Ser Leu Gly Gly Ser Ser Val Arg Lys Leu
370 375 380
Ser Phe Glu Gln Asn Lys Ile Thr Glu Asn Ala Phe Ser His Ala Ser
385 390 395 400
Gly Asn Leu Lys Ser Gly Ile Leu Leu Thr Gln Pro Arg Arg Cys Ser
405 410 415
Trp Gly Leu Lys Asp Glu Lys Tyr Arg Glu Arg Leu Gly Lys Ser Leu
420 425 430
Gly Gly Ser Ser Val Arg Lys Leu Ser Phe Glu Gln Ala Lys Ile Thr
435 440 445
Glu Asn Ala Phe Ser His Ala Ser Gly Asn Leu Ile Glu Glu Pro Val
450 455 460
Thr Cys Thr Asp Asp Asn Val Glu Ser His Ser Gly Val Leu Leu Thr
465 470 475 480
Gln Pro Arg Val Ser Leu Gly Ile Asn Glu Asp Gln Arg Gly Lys Trp
485 490 495
Gly Glu Asn Met Val Asp Ser Ser Pro Arg Gln Leu Ser Phe Glu Tyr
500 505 510
Lys Asn Gln Lys Glu Asp Pro Val Asp Glu Asn Pro Glu Asn Leu Lys
515 520 525
Asp Lys Ala Lys Lys Ser Trp Ala Asp Ile Ala Glu Glu Asp Glu Glu
530 535 540
Met Tyr Lys Glu Leu Val Cys Gly Asp Thr Pro Ser Lys Tyr Phe Asp
545 550 555 560
Gly Leu Lys Ser Lys Glu Glu Glu Glu Lys Phe Ser Asp Glu Asn Leu
565 570 575
Asp Thr Asn Leu Met Tyr Gln Ser Pro Tyr Tyr Pro Leu Ser Gln Thr
580 585 590
Glu Ala Ala Gly Gln Glu Leu Lys Ser Phe Tyr Gln Lys Asp Gly Tyr
595 600 605
Asn Gly Thr Ser Gly Asn Ala Val Ser Ser Arg Asn Pro Thr Ala Arg
610 615 620
Arg Ser Leu Tyr Phe Gly Gly Asp Ser Asn Ser Leu Pro Leu Lys Glu
625 630 635 640
Lys Asp Ser Phe Thr Gly Lys Asn Ile Ser Met Thr Arg Arg Asn Arg
645 650 655
Leu Lys Val Phe Thr Glu Ile Thr Leu Leu Pro Asp Ser Pro
660 665 670
<210> 5
<211> 676
<212> PRT
<213> 可可树（Theobroma cacao）
<400> 5
Met Trp Ser Asn Glu Lys Asn Leu Pro Ser Arg Ser Arg Gly Phe Phe
1 5 10 15
Thr Pro Gln Pro Pro Ala Trp Lys Lys Ala Gln Ser Ser Val Leu Pro
20 25 30
Met Ser Glu Arg Lys Arg Ile Ser Pro Ala Asp Gln Ala Asp Ser Phe
35 40 45
His Val Ile His Lys Val Pro Ala Ser Asp Ser Pro Tyr Gly Arg Ala
50 55 60
Lys His Val Gln Leu Ile Asp Lys Asp Pro Ser Lys Ala Ile Ser Leu
65 70 75 80
Phe Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Asp Ser Ala Leu Lys
85 90 95
Asp Met Ala Val Val Met Lys Gln Leu Asn Arg Ser Asp Glu Ala Ile
100 105 110
Glu Ala Ile Lys Ser Phe Arg His Leu Cys Pro His Asp Ser Gln Glu
115 120 125
Ser Leu Asp Asn Val Leu Val Glu Leu Tyr Lys Arg Ser Gly Arg Val
130 135 140
Glu Glu Glu Ile Glu Met Leu Gln Asn Lys Leu Arg Asn Ile Glu Gly
145 150 155 160
Gly Thr Val Phe Gly Gly Lys Arg Thr Lys Ile Ala Arg Ser Gln Gly
165 170 175
Lys Lys Ile Gln Ile Thr Ile Glu Gln Glu Lys Ser Arg Ile Leu Gly
180 185 190
Asn Leu Ala Trp Ala Tyr Leu Gln Gln His Asn Tyr Gly Ile Ala Glu
195 200 205
Gln His Tyr Arg Lys Ala Leu Ser Leu Glu Pro Asp Met Asn Lys Gln
210 215 220
Cys Asn Leu Ala Ile Cys Leu Met His Met Asn Arg Ile Ser Glu Ala
225 230 235 240
Lys Ser Leu Leu Gln Asp Val Lys Ala Ser Ser Gly Asn Glu Gln Met
245 250 255
Asp Glu Ser His Ser Lys Ser Tyr Glu Arg Ala Leu Asp Met Leu Ile
260 265 270
Gln Val Glu Ser Gln Ser Met Leu Glu Pro Val Ala Lys Glu Pro Asp
275 280 285
Lys Gly Arg Glu Ile Gln Arg Pro Ser Thr Pro Cys Arg Asp Arg Gly
290 295 300
Leu Lys Glu Ala Gly Ile Phe Leu Pro Arg Asn Glu Asp Asn Ile Ser
305 310 315 320
Gly Phe Met Gly Arg Arg Arg Leu Pro Tyr Ala His Trp Glu Gly Lys
325 330 335
Met Leu Ile Asp Glu Gln Asn Gly Glu Ser Tyr Arg Arg Asn Pro Leu
340 345 350
Glu Lys Asn Asp Asn Phe Pro Gly Tyr Asp Asp Arg Ser Ser Lys Cys
355 360 365
Thr Pro Ile Gly Gln Lys Gly Tyr Leu Gln Ser Ser Pro Gln Ser Met
370 375 380
Phe Thr Glu Lys Trp Arg Ile Gly Ser Tyr Trp Glu Ser Pro Cys Glu
385 390 395 400
Gly Tyr Ser Thr Gly Glu Glu Val Gly Ser Ala Gln Lys Lys Ile Tyr
405 410 415
Ala Ser Ser Ala Ala Ser Lys Lys Asn Ser Glu Ala Leu Phe Thr Gln
420 425 430
Pro Arg Arg Cys Ser Gln Gly Phe Asn Asn Ala Asp Gln Lys Arg Gly
435 440 445
Gly Arg Trp Gly Glu Asp Thr Val Arg Asn Ser Ile Arg Lys Leu Ser
450 455 460
Phe Glu Gln Ser Leu Thr Ser Glu Ser Glu Pro Leu His Ser Ile Gln
465 470 475 480
Asn Leu Asn Glu Lys Pro Gln Ala Ser Asn Asn Gly Lys Ser Glu Asn
485 490 495
Ser Ala Thr Gly Pro Val Glu Glu Glu Val Gln Glu Gly Leu Ser Gly
500 505 510
Val Leu Phe Thr Gln Pro Arg Asn Ser Leu Leu Trp Leu Asn Asn Arg
515 520 525
Asp Gln Arg Met Glu Arg Trp Ala Glu Glu Ser Val Gly Cys Pro Phe
530 535 540
Arg Lys Leu Ser Phe Glu Lys Asn Ile Thr Gly Val Thr Pro His Ser
545 550 555 560
Ala Asp Gly Leu Asn Gly Glu Pro Leu Phe Ser Ser Lys Asp Glu Ser
565 570 575
Glu Ile Gly Leu Glu Arg Pro Ala Asn Ala Pro Asn Lys Lys Ser Trp
580 585 590
Ala Asp Met Val Glu Glu Glu Glu Lys Glu Glu Leu Leu Asn Ser Tyr
595 600 605
Asp Gly Phe Asn Arg Glu Glu Val Phe Asn Asp Glu Asn Leu Asn Ser
610 615 620
Asn Ile Ile Tyr Pro Arg Pro Asp Cys Lys Asp His Ile Gly Asn Ile
625 630 635 640
Thr Gln Gln Leu Glu Ser Phe Asp Met Lys Gly Gly Asp Asn Ala Ser
645 650 655
Ala Asn Thr Val Ser Ser Arg Arg Asn Arg Leu Pro Val Phe Arg Asp
660 665 670
Ile Thr Ser Ser
675
<210> 6
<211> 631
<212> PRT
<213> 木薯（Manihot esculenta）
<400> 6
Met Trp Ser His His Glu Asn Asn Ile Pro Ala Arg Gly Phe Ser Thr
1 5 10 15
Pro Pro Pro Ser Trp Lys Ser Pro His Cys Leu Ala Ala Met Pro Met
20 25 30
Ser Glu Arg Lys Ser Val Ser Ser Gly Cys Lys Arg Asp Leu Phe His
35 40 45
Val Ile His Lys Val Pro Ala Gly Asp Ser Pro Tyr Val Arg Ala Lys
50 55 60
His Val Gln Leu Ile Glu Lys Asp Pro Ser Arg Ala Ile Ser Leu Phe
65 70 75 80
Trp Ala Ala Ile Asn Ala Gly Asp Arg Ile Asp Ser Ala Leu Lys Asp
85 90 95
Met Ala Val Val Met Lys Gln Leu Asn Arg Ser Asp Glu Ala Ile Glu
100 105 110
Ala Ile Lys Ser Phe Arg Arg Leu Cys Pro Tyr Asp Ser Gln Glu Ser
115 120 125
Ile Asp Asn Val Leu Val Glu Leu Tyr Lys Arg Ser Gly Arg Ile Glu
130 135 140
Glu Glu Ile Glu Met Leu His Leu Lys Leu Lys Asn Ile Glu Glu Gly
145 150 155 160
Ile Ala Phe Gly Gly Lys Arg Thr Lys Thr Ala Arg Ser Gln Gly Lys
165 170 175
Lys Ile Gln Ile Thr Val Glu Gln Glu Arg Ser Arg Ile Leu Gly Asn
180 185 190
Leu Ala Trp Ala Tyr Leu Gln His His Asp Tyr Gly Leu Ala Glu Gln
195 200 205
Tyr Tyr Arg Lys Ala Leu Ser Leu Glu Pro Asp Lys Asn Lys Gln Cys
210 215 220
Asn Leu Ala Ile Cys Leu Met His Met Asn Arg Ile Pro Glu Ala Lys
225 230 235 240
Ser Leu Leu Gln Ala Val Ser Asp Ser Cys Gly Ser Lys Gln Met Asp
245 250 255
Asp Ser Tyr Ala Lys Ser Phe Glu Arg Ala Val Asp Ile Leu Thr Glu
260 265 270
Leu Glu Ser Lys Ser Val Leu Lys Pro Ala Glu Glu Asp Lys Glu Asn
275 280 285
Gln Arg Ser Leu Ala Ser Pro Leu Leu Ser Asp Lys His Asp Arg Gly
290 295 300
Ser Tyr Cys Gly Ile His Ser Glu Glu Gln Gly Asn Phe Ile Ser Ser
305 310 315 320
Ser Gly Lys Gly Pro Gly Leu Ala Asn Lys Arg Met Leu Asp Ser Pro
325 330 335
Ala Ala Val Leu Tyr Thr Gln Pro Lys Arg Val Met Gly Arg Ser Asp
340 345 350
Glu Glu Glu Gln Arg Arg Gly Val Gly Trp Glu Asn Asp Thr Val Glu
355 360 365
Lys Pro Ser Lys Asn Val Ser Ala Cys Ile Ile Arg Ser Leu Asp Gly
370 375 380
Glu Leu Leu Asp Pro Pro Ala Glu Arg Asn Trp Arg Glu Lys Ser Trp
385 390 395 400
Thr Glu Val Ala Gln Gly Lys Ile Thr Gly Val Thr Val Pro Tyr Gln
405 410 415
Phe Ser Gln Pro Arg Ile Arg Ala Phe Thr Gly Tyr Asn Asp Ala His
420 425 430
Leu Lys Asp Glu Asn Val Thr Lys Ser Asp Ser Gln Gln Pro Ser Trp
435 440 445
Arg Ser Asn Ala Arg Glu Thr Gly Gly Gln Met Gly Ser Thr Asn Glu
450 455 460
Lys Ser Asp Ala Ser Ser Lys Phe His Leu Glu Gln Asn Met Val Val
465 470 475 480
Asp Asp Ala Arg Gln Ser Glu Thr Ser Ile Asp Gly Lys Cys Gly Gln
485 490 495
Thr Phe Gly Ile Asn Gly Cys Leu Gly Lys Asn Ser Ser Ser Lys Phe
500 505 510
Ala Ile Lys Ser Trp Ala Asp Met Val Glu Glu Glu Glu Glu Lys Leu
515 520 525
Leu Thr Gly Lys Asp Leu Ser Pro Tyr Phe Asp Gly Gly Trp Asp Tyr
530 535 540
Glu Glu Glu Ser Ala Asp Glu Asn Gln Asp Ser Asn Ile Ile His Gln
545 550 555 560
Thr Ser Cys Pro Lys Ser Pro Ala Glu Ala Ile Asn Gln Lys Phe Glu
565 570 575
Ala Phe Asp Leu Lys Asp Gly Phe Ala Ala Phe Ser Asn Ala Val Ser
580 585 590
Pro Arg Asn Pro Thr Val Arg Arg Ser Leu Arg Phe Asp Ala Lys Asn
595 600 605
Asp Phe Ser Thr Arg Lys Lys His Arg Leu Gln Val Phe Gln Asp Ile
610 615 620
Thr Pro Ser Ile Asp Ser Pro
625 630
<210> 7
<211> 673
<212> PRT
<213> 野草莓（Fragaria vesca）
<400> 7
Met Trp Ala Arg Asp Arg Ser Tyr Pro Pro Ala Gly Phe Phe Thr Pro
1 5 10 15
Pro Pro Pro Arg Lys Ser Gly Pro Pro Asn Pro Pro Pro Met Ser Glu
20 25 30
Arg Lys Arg Val Ser Pro Ala Ala Ala Arg Ser Asp Leu Phe His Val
35 40 45
Ala His Lys Val Pro Ala Gly Asp Ser Pro Tyr Val Arg Ala Lys Gln
50 55 60
Val Gln Leu Ile Asp Lys Asn Pro Ser Lys Ala Ile Ser Leu Phe Trp
65 70 75 80
Ala Ala Ile Asn Ala Gly Asp Arg Val Asp Ser Ala Leu Lys Asp Met
85 90 95
Ala Val Val Met Lys Gln Leu Asp Arg Ala Glu Glu Ala Ile Glu Ala
100 105 110
Ile Lys Ser Phe Arg His Leu Cys Pro Tyr Glu Ser Gln Glu Ser Leu
115 120 125
Asn Asn Val Leu Val Glu Leu Tyr Lys Arg Ala Gly Arg Ile Glu Asp
130 135 140
Glu Ile Glu Thr Leu Gln Ser Lys Leu Lys Arg Met Asp Glu Gly Ile
145 150 155 160
Ala Phe Asn Gly Lys Arg Thr Lys Thr Ala Arg Ala Gln Gly Lys Lys
165 170 175
Val Gln Ile Thr Val Glu Gln Glu Arg Ser Arg Val Leu Gly Asn Leu
180 185 190
Ala Trp Ala Tyr Met Gln Gln Gly Asn Tyr Thr Thr Ala Glu Glu His
195 200 205
Tyr Lys Asn Ala Leu Ala Leu Glu Pro Asp Lys Asn Lys Gln Cys Asn
210 215 220
Leu Ala Ile Cys Leu Met His Met Asn Arg Ile Thr Glu Ala Arg His
225 230 235 240
Leu Leu Gln Ala Val Arg Asp Ser Ala Gly Asn Lys Pro Met Asp Glu
245 250 255
Ser Tyr Ala Lys Ser Phe Glu Arg Ser Phe Glu Met Leu Thr Glu Leu
260 265 270
Glu Gln Gln Ser Val Leu Arg Pro Ile Gln Gln Asn Glu Asn Tyr Cys
275 280 285
Thr Gly Ile Ser Arg Thr Pro Lys Pro Ser Gly Ser Gly Tyr Met Pro
290 295 300
Met Pro Pro Arg Arg Trp Thr Asp Gly Pro Glu Tyr Val Thr Val Ala
305 310 315 320
Asn Glu Arg His Arg Glu Ser Phe Arg Pro Gly Ser Cys Ala Lys Ser
325 330 335
Phe Asp Lys Trp Lys Lys Asp Tyr Asp Leu Lys Asn Thr Gly Asp Ser
340 345 350
Arg Pro Ser Phe Ser Ser Ser Val Lys Gln Asn Gln Asp Gly Met Thr
355 360 365
Val Thr Glu Val Asn Pro His Glu Lys Thr Tyr Val Ser Pro Val Leu
370 375 380
Tyr Thr Gln Pro Arg Arg Pro Ser Trp Gly Phe Asn Asp Gly His Gln
385 390 395 400
Arg Ser Glu Ile Trp Gly Asn Gly Val Gly Ser Ser Asn Lys Lys Leu
405 410 415
Pro Pro Glu Arg Ser Ala Gly Asn Val Arg Ala His Val Val Arg Asn
420 425 430
Leu Asn Ala Asp Leu Leu Ala Ser Thr Pro Arg Glu Ser Asp Ser Ser
435 440 445
Leu Ser Ser Arg Ser His Gly Asp Trp Arg Met Pro Gln Arg Asp Ala
450 455 460
Val Ala Arg Pro Val Leu Gln Pro Val Ser Ser Met Thr Ser Arg Gly
465 470 475 480
Asn Glu Gly Tyr Leu Pro Val Arg Asn Glu Ala Met Ile Asp Arg Ser
485 490 495
Ser Lys Tyr Thr Gly Asn Gly Asp Ile Arg Arg Val Thr Trp Asp Asn
500 505 510
Ala Gly Met Gln Lys Ser Ala Ala Gly Ser Gln Val Val Asp Glu Asn
515 520 525
Ala Lys Ala Leu Leu Leu Cys Ile Asp Glu Gly Asp Ser Gln Ser Ser
530 535 540
Gly Thr Thr Val Leu Gly Ser Met Glu Asn Met Asn Ala Ser Val Glu
545 550 555 560
Glu Asp Cys Leu Gly Asp Asn Ser Ser Gly Thr Leu Asp Asn Val His
565 570 575
Gln Ser Pro Ala Glu Asn Glu Arg Pro Thr Leu His Gln Ser Pro Ala
580 585 590
Glu Thr Glu Lys Pro Thr Pro Asp Phe Trp Lys Tyr Ser Gly Lys Lys
595 600 605
Ser Trp Ala Asp Met Val Glu Glu Glu Glu Glu Glu Leu Arg Ser Gly
610 615 620
Thr Thr Gly Tyr Phe Asp Ser Trp Asn Thr Gly Asp Glu Phe Asp Asp
625 630 635 640
Glu Asn Arg Asn Pro Asn Ile Met Thr Pro Gln Ser Pro Gln Phe Gln
645 650 655
Thr Gln Met Lys Arg Arg Asn Arg Leu Gln Ser Phe Gln Asp Ile Thr
660 665 670
Asn
<210> 8
<211> 601
<212> PRT
<213> 大豆（Glycine max）
<400> 8
Met Leu Phe Glu Arg Gly Ser Pro Ala Arg Cys Tyr Met Thr Pro Pro
1 5 10 15
Pro Gln Arg Thr Ser Pro Trp Lys Pro Pro His Ser Arg Ser Ser Ser
20 25 30
Val Pro Phe Ser Glu Arg Lys Met Ser Pro Asn Ser Val Asn Lys Ser
35 40 45
Asp Ile Phe His Ile Ile His Lys Val Pro Ala Gly Asp Ser Pro Tyr
50 55 60
Val Lys Ala Lys Gln Val Gln Leu Val Asp Lys Asp Pro Gly Arg Ala
65 70 75 80
Val Ser Leu Phe Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Glu Ser
85 90 95
Ala Leu Lys Asp Met Ala Leu Val Met Lys Gln Leu Asn Arg Ser Asp
100 105 110
Glu Ala Ile Glu Ala Ile Arg Ser Phe Arg His Leu Cys Pro Ser Asp
115 120 125
Ser Gln Asp Ser Leu Asp Asn Ile Leu Val Glu Leu Tyr Lys Arg Ser
130 135 140
Gly Arg Val Asp Glu Glu Ile Ala Met Leu Cys His Lys Leu Lys Gln
145 150 155 160
Ile Glu Asp Gly Leu Thr Phe Val Gly Arg Thr Thr Lys Gln Ala Arg
165 170 175
Ser Gln Gly Lys Lys Ile Gln Ile Thr Ala Glu Gln Glu Ile Ser Arg
180 185 190
Ile Leu Gly Asn Leu Ala Trp Ala Tyr Leu Gln Lys Gly Asp Tyr Lys
195 200 205
Thr Ala Glu Glu His Tyr Arg Lys Ala Leu Ser Phe Glu Val Asp Arg
210 215 220
Asn Lys Gln Cys Asn Leu Ala Ile Cys Leu Ile His Met Asn Lys Ile
225 230 235 240
Lys Glu Ala Lys Phe Leu Leu Gln Ala Val Arg Thr Ala Thr Lys Asn
245 250 255
Arg Lys Met Asp Asp Ser Phe Ala Lys Ser Phe Glu Arg Ala Ser Gln
260 265 270
Met Leu Ile Glu Ile Glu Thr Ser Ser Ser Gln Asn Ala Ala Phe Ser
275 280 285
Met Thr Thr Gln Cys Pro Pro Gln Ser Phe Glu Asn Ser Ile Arg Met
290 295 300
Ser Ser Asp Ser Val Gln Ser Arg Ser Glu Asn Arg Ser Glu Ile Ser
305 310 315 320
Glu Gly Asp Ala Pro His Ala Arg Arg Arg Leu Tyr Gln Ser Pro Asp
325 330 335
Pro Ser Arg Arg Asp Leu Ser Val Pro Cys Thr Lys Pro Lys Arg Cys
340 345 350
Ser Trp Gly Phe Asn Asn Gly His Arg Arg Glu Ala Trp Gly Asp Ala
355 360 365
Asn Ser Asp Tyr Lys Pro Thr Phe Gly Thr Pro Pro Asn Asp Lys His
370 375 380
Val Thr Arg Met Leu Asn Ser Arg Glu Asn Gly Phe Ser Ser Pro Ala
385 390 395 400
Asn Gly Asn Gly Lys Ala Ala Thr Leu Glu Asp Pro Ala Ile Leu Lys
405 410 415
His Glu Ala Thr Lys Val Thr Ser Ser Asp Ser Leu His Thr Leu Asn
420 425 430
Thr Glu Ser Ala Thr Glu Phe Thr Glu Lys Glu Lys Ser Ala Ala Asp
435 440 445
Asp Ser Ser Tyr Gly Ser Ile Leu Ser Glu Ser His Val Thr Val Val
450 455 460
Asn Gly Val Asn Glu Phe Ala Ser Gly Lys Arg Lys Pro Glu Lys Lys
465 470 475 480
Ser Trp Ala Asp Ile Val Glu Glu Glu Gln Asn Glu Glu Asn Asp Phe
485 490 495
Phe Ser Gly Phe Thr Asn Phe Asp Asp Lys Asn Gly Ala Glu Val Phe
500 505 510
Asn Asp Glu Asn Glu Asp Ser Asn Ile Ile Tyr Pro Ser Pro Trp Pro
515 520 525
Gln Asn Gln Pro Asp Glu Trp Ser Ser Lys Lys Leu Glu Ser Leu Asp
530 535 540
Lys Lys Asp Gly His Tyr Ala Ser Glu Ser Ala Ile Leu Ser Arg Asn
545 550 555 560
Pro Thr Ala Arg Arg Ser Leu Cys Phe Asn Pro Glu Leu Ile Gly Glu
565 570 575
Lys Arg Leu Thr Arg Arg Ser Arg Leu Gln Val Phe Gln Asp Ile Thr
580 585 590
Leu Leu Pro Glu Thr Pro Arg Phe Ala
595 600
<210> 9
<211> 602
<212> PRT
<213> 大豆（Glycine max）
<400> 9
Met Gln Phe Glu Arg Gly Ser Met Ala Arg Cys Tyr Met Thr Pro Pro
1 5 10 15
Pro Gln Pro Pro Ser Ser Trp Lys Pro Pro His Ser Gln Ser Pro Ser
20 25 30
Val Pro Phe Ser Glu Arg Lys Lys Ser Pro Asn Ser Ala Asn Lys Ile
35 40 45
Asp Leu Phe His Ile Ile His Lys Val Pro Ala Gly Asp Ser Pro Tyr
50 55 60
Val Lys Ala Lys Gln Val Gln Leu Val Asp Lys Asp Pro Gly Arg Ala
65 70 75 80
Ile Ser Leu Phe Trp Ala Ala Ile Asn Ala Arg Asp Arg Val Glu Ser
85 90 95
Ala Leu Lys Asp Met Ala Leu Val Met Lys Gln Leu Asn Arg Ser Asp
100 105 110
Glu Ala Ile Glu Ala Ile Arg Ser Phe Arg His Leu Cys Pro Ser Asp
115 120 125
Ser Arg Asp Ser Leu Asp Asn Ile Leu Val Glu Leu Tyr Lys Arg Ser
130 135 140
Gly Arg Ile Asp Glu Glu Ile Ala Met Leu His His Lys Leu Lys Gln
145 150 155 160
Ile Glu Asp Gly Leu Thr Phe Val Gly Arg Thr Thr Lys Gln Ala Arg
165 170 175
Ser Gln Gly Lys Lys Ile Gln Ile Thr Ala Glu Gln Glu Ile Ser Arg
180 185 190
Ile Leu Gly Asn Leu Ala Trp Ala Tyr Leu Gln Lys Gly Asp Tyr Lys
195 200 205
Ala Ala Glu Glu His Tyr Arg Lys Ala Leu Ser Phe Glu Val Asp Arg
210 215 220
Asn Lys Gln Cys Asn Leu Ala Ile Cys Leu Ile His Met Asn Lys Ile
225 230 235 240
Lys Glu Ala Lys Phe Leu Leu Gln Ala Val Arg Thr Ala Thr Lys Asn
245 250 255
Arg Lys Met Asp Asp Ser Phe Ala Lys Ser Phe Glu Arg Ala Ser Gln
260 265 270
Met Leu Ile Glu Ile Glu Thr Ser Ser Ser Ser Gln Asn Ala Ala Phe
275 280 285
Ser Met Thr Thr Met Ser Gln Cys Ser Pro Gln Ser Phe Glu Asn Ser
290 295 300
Ile Arg Lys Ser Ser Asp Arg Val Gln Ser Gly Ser Glu Ser Arg Ser
305 310 315 320
Glu Thr Ser Glu Gly Asp Val Pro His Ala Arg Arg Arg Leu Tyr Gln
325 330 335
Ser Pro Asp Pro Ser Arg Arg Asp Leu Ser Val Pro Cys Thr Lys Pro
340 345 350
Lys Arg Cys Ser Trp Gly Phe Asn Thr Gly Tyr Arg Arg Glu Ala Trp
355 360 365
Gly Asp Val Asn Ser Asp Tyr Lys Pro Ser Phe Gly Thr Pro Pro Asn
370 375 380
Asp Lys His Ala Thr Arg Met Leu Asn Ser Arg Glu Asn Gly Leu Ser
385 390 395 400
Ser Pro Ala Asn Gly Lys Trp Arg Ala Met Thr Leu Glu Asp Gln Ala
405 410 415
Ile Leu Lys His Glu Ala Thr Thr Ala Thr Ser Ser Asp Ser Leu His
420 425 430
Ala Leu Asn Thr Glu Val Ala Met Glu Phe Thr Glu Lys Glu Lys Ser
435 440 445
Ala Ala Asp Asp Ser Ser Tyr Arg Ser Ile Leu Ser Glu Ser His Val
450 455 460
Thr Val Val Asn Gly Ala Asn Glu Phe Ala Ser Gly Lys Arg Lys Pro
465 470 475 480
Glu Lys Lys Ser Trp Ala Asp Ile Val Glu Glu Glu Gln Asn Glu Glu
485 490 495
Tyr Asp Phe Leu Ser Gly Phe Thr Asn Phe Asp Asp Lys Asn Gly Ala
500 505 510
Glu Val Phe Asn Asp Glu Asn Glu Asp Ser Asn Ile Ile Tyr Pro Ser
515 520 525
Ser Trp Leu Gln Asn Leu Pro Glu Trp Ser Asn Lys Lys Leu Glu Ser
530 535 540
Ser Asp Lys Lys Asp Asp Tyr Ala Ser Glu Ser Thr Ile Leu Ser Arg
545 550 555 560
Asn Pro Thr Ala Arg Arg Ser Leu Cys Phe Asn Pro Glu Leu Ile Gly
565 570 575
Glu Lys Lys Leu Thr Arg Arg Ser Arg Leu Gln Val Phe Gln Asp Ile
580 585 590
Thr Leu Leu Pro Glu Thr Pro Arg Phe Ala
595 600
<210> 10
<211> 603
<212> PRT
<213> 莲藕（Lotus japonicus）
<400> 10
Met Pro Phe Glu Arg Asn Ser Pro Ala Arg Cys Phe Met Thr Pro Pro
1 5 10 15
Pro Pro Arg Leu Ser Ser Trp Arg Ser Thr Pro Ser Arg Ser Pro Ile
20 25 30
Met Met Pro Leu Ser Glu Arg Lys Arg Ser Ser Pro Asn Lys Asp Asp
35 40 45
Pro Tyr His Val Ile His Lys Val Pro Ala Gly Asp Ser Pro Tyr Val
50 55 60
Lys Ala Lys Gln Val Gln Leu Val Asp Lys Asp Pro Gly Lys Ala Ile
65 70 75 80
Ser Leu Phe Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Glu Ser Ala
85 90 95
Leu Lys Asp Met Ala Leu Val Met Lys Gln Leu Asn Arg Ser Asp Glu
100 105 110
Ala Ile Glu Ala Ile Lys Ser Phe Arg His Leu Cys Pro Pro Asp Ser
115 120 125
Gln Glu Ser Leu Asp Asn Ile Leu Val Glu Leu Tyr Lys Arg Ser Gly
130 135 140
Arg Val Asp Glu Glu Ile Ser Met Leu His His Lys Leu Lys Gln Ile
145 150 155 160
Glu Asp Gly Val Thr Phe Val Gly Arg Thr Thr Lys Gln Ala Arg Ser
165 170 175
Gln Gly Lys Lys Ile His Val Thr Ala Glu Gln Glu Ile Ser Arg Ile
180 185 190
Leu Gly Asn Leu Ala Trp Ala Tyr Leu Gln Lys Gly Asp Tyr Lys Ala
195 200 205
Ala Glu Glu His Tyr Arg Lys Ala Leu Ser Phe Glu Val Asp Arg Asn
210 215 220
Lys Gln Cys Asn Leu Ala Ile Cys Leu Met Gln Thr Asn Arg Ile Thr
225 230 235 240
Glu Ala Lys Phe Leu Leu Gln Ala Val Thr Thr Ala Ser Lys His Arg
245 250 255
Lys Met Asp Asp Ser Cys Ala Lys Ser Phe Glu Arg Ala Ser Gln Met
260 265 270
Leu Met Asp Met Glu Ser Ser Ser Ser Gln Cys Ser Pro Leu Ser Asn
275 280 285
Leu Ser Gly Lys Ser Ser Asp Met Val Gln Ser Arg Thr Gln Asn Trp
290 295 300
Ser Val Thr Ser Glu Gly Glu Val Ser Asn Ala Arg Arg Arg Leu Tyr
305 310 315 320
Glu Ser Pro Glu Pro Ala Arg Arg Asp Leu Lys Val Pro Tyr Thr Asn
325 330 335
Thr Lys Lys Cys Ser Trp Gly Phe Asn Asn Gly Pro Gln Arg Glu Thr
340 345 350
Trp Gly Asp Val His Ser Asp Pro Lys Pro Ser Phe Gly Ile Pro Ser
355 360 365
Lys Gln Asn Asn Leu Ser Leu Pro Ala Asn Gly Lys Trp Asn Ala Arg
370 375 380
Thr Met Val Lys Arg Glu Asp Lys Thr Ala Ile Gly Ser Gly Leu Tyr
385 390 395 400
Thr Tyr Ser Asn Thr Glu Thr Asp Arg Lys Phe Lys Glu Asp Lys Phe
405 410 415
Ala Val Glu Ser Asn Asp Met Val Val Asn Gly Ala Asn Gln Phe Ala
420 425 430
Ser Ser Ile Gly Thr Asp Gln Ser Lys Cys Ser Glu Ile Ser Ile Lys
435 440 445
Leu Cys Cys Tyr Pro Phe Cys Gln Leu Asn Ser Ser Leu Ile Asn Leu
450 455 460
His Glu Leu Ala Ser Gly Ser Ile Lys Pro Val Glu Lys Lys Ser Trp
465 470 475 480
Ala Asp Ile Val Glu Glu Glu Gln Asn Glu Gly His Asp Phe Phe Gly
485 490 495
Gly Tyr Thr Arg Phe Asp Gly Gln Glu Gly Ala Gln Val Phe Asn Lys
500 505 510
Glu Asn Glu Asn Ser Asn Ile Val Phe Gln Arg Pro Trp Pro Leu Ser
515 520 525
Glu Thr Lys Cys Ile Ser Lys Lys Leu Glu Ser Met Asp Leu Lys Asp
530 535 540
Gly His His Gly Ser Gly Thr Val Thr Leu Ser Arg Lys Pro Ala Val
545 550 555 560
Arg Arg Ser Leu Cys Phe Asn Pro Glu Leu Ala Lys Glu Arg Asp Ser
565 570 575
Ser Leu Ser Glu Glu Lys Lys Pro Pro Arg Asn Asn Arg Leu Gln Val
580 585 590
Phe Lys Asp Ile Ala Leu His Pro Glu Thr Pro
595 600
<210> 11
<211> 617
<212> PRT
<213> 蒺藜苜蓿（Medicago truncatula）
<400> 11
Met Thr Phe Glu Arg Asn Ser Pro Ala Arg Cys Tyr Met Thr Pro Pro
1 5 10 15
Ser Ser Ser Ser Ser Trp Lys Ser Arg Pro Val Arg Ser Pro Thr Val
20 25 30
Pro Phe Ser Glu Arg Lys Lys Ser Pro Ala Ala Ser Val Ser Lys Asp
35 40 45
Asp Leu Phe His Val Ile His Lys Val Pro Ser Gly Asp Ser Pro Tyr
50 55 60
Val Lys Ala Lys Gln Val Gln Leu Val Asp Lys Asp Pro Gly Lys Ala
65 70 75 80
Ile Ser Leu Phe Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Glu Ser
85 90 95
Ala Leu Lys Asp Met Ala Leu Val Met Lys Gln Leu Asn Arg Ser Asp
100 105 110
Glu Ala Ile Glu Ala Ile Lys Ser Phe Arg His Leu Cys Pro Ser Asp
115 120 125
Ser Gln Glu Ser Leu Asp Asn Ile Leu Val Glu Leu Tyr Lys Arg Ser
130 135 140
Gly Arg Val Asp Glu Glu Ile Gly Met Leu His Gln Lys Leu Lys Gln
145 150 155 160
Ile Glu Asp Gly Met Thr Tyr Val Gly Arg Thr Thr Lys His Ala Arg
165 170 175
Ser Gln Gly Lys Lys Ile Gln Ile Ser Ala Glu Gln Glu Ile Ser Arg
180 185 190
Ile Leu Gly Asn Leu Ala Trp Ala Tyr Leu Gln Lys Gly Asp Tyr Lys
195 200 205
Thr Ala Glu Glu His Tyr Arg Lys Ala Leu Ser Phe Glu Val Asp Arg
210 215 220
Asn Lys Gln Cys Asn Leu Ala Ile Cys Leu Met Gln Met Asn Lys Val
225 230 235 240
Thr Glu Ala Arg Phe Leu Leu Gln Ala Val Thr Ala Ala Thr Lys Asn
245 250 255
Arg Lys Met Asp Asp Ser Phe Val Lys Ser Tyr Glu Arg Ala Thr Gln
260 265 270
Met Leu Gln Glu Met Glu Ser Thr Ala Pro Ser Val Asp Ser Val Lys
275 280 285
Asp Lys Gly Asp Asn Lys Phe Asn Glu Thr Glu Arg Phe Ser Gly Arg
290 295 300
Asn Met Ser Ser Pro Tyr Ser Thr Pro Asn Leu Glu Ser Ser Asn Gly
305 310 315 320
Lys Thr Thr Gly Thr Val Lys Ser Arg Thr Glu Asn Asn Arg Ser Leu
325 330 335
Thr Ser Asp Ala Lys Asp Ser His His Ser His Ala Arg Arg Arg Leu
340 345 350
Tyr Glu Ser Leu Asp Pro Ala Lys Ser Asp Pro Lys Val Pro Pro Tyr
355 360 365
Thr Lys Pro Lys Arg Pro Ser Trp Gly Phe Asn Ser His Ser Asp Ser
370 375 380
Lys Pro Ser Phe Val Ser Tyr Pro Asn Glu Lys Ala Pro Tyr Ile Ile
385 390 395 400
Lys Pro Asn Ser Thr Gln Asn Gly Phe Ser Pro Arg Thr Ala Thr Asn
405 410 415
Trp Arg Thr Arg Thr Pro Glu Gly Asp Ala Ala Ile Val Lys Tyr Gly
420 425 430
Pro Thr Thr Thr Val Lys Gln Gly Asn Lys Thr Thr Val Phe Ser Ser
435 440 445
Gly Ser Ile Tyr Pro Leu Asn Thr Glu Ala Ala Met Lys Phe Thr Lys
450 455 460
Asn Asp Asn Asn Asn Lys Phe Thr Val Thr Asn Glu Phe Ala Ala Ser
465 470 475 480
Val Asp Thr Lys Asp Gln Asn Gln Asp Lys Lys Leu Ala Lys Lys Ser
485 490 495
Trp Ala Asp Met Val Glu Glu Glu Gln Gln Ser Glu Glu Tyr Glu Leu
500 505 510
Phe Tyr Lys Gly Tyr Thr Asn Phe Asp Ala Gln Val Phe Gln Asn Glu
515 520 525
Asn Glu Asn Ser Asn Ile Val Tyr Gln Pro Pro Ser Arg Arg Ser His
530 535 540
Tyr Glu Thr Glu Ser Leu Asn Gln Asn Leu Glu Phe Met Asn Leu Lys
545 550 555 560
Asp Gly Tyr Asn Ala Ala Pro Val Asn Asp Thr Trp Leu Arg Asn Pro
565 570 575
Thr Val Arg Arg Ser Leu Phe Thr Asn Ala Glu Met Thr Asn Glu Arg
580 585 590
Asp Val Phe Ser Gly Glu Glu Lys Arg Thr Arg Arg Ala Arg Leu Gln
595 600 605
Val Phe Gln Asp Ile Thr Ser Ser Ser
610 615
<210> 12
<211> 673
<212> PRT
<213> 葡萄（Vitis vinifera）
<400> 12
Met Trp Ser His Asn Asn Asn Phe Pro Ala Lys Gly Phe Ser Thr Pro
1 5 10 15
Pro Pro Thr Trp Lys Ser Lys Ala Ser Phe Ser Ser Ala Thr Pro Val
20 25 30
Ser Glu Lys Thr Arg Ser Met Ala Asn Lys Asp Asp Leu Phe His Val
35 40 45
Val His Lys Val Pro Ala Gly Asp Ser Pro Tyr Val Arg Ala Lys Gln
50 55 60
Val Gln Leu Ile Asp Lys Asp Pro Asn Arg Ala Ile Ser Leu Phe Trp
65 70 75 80
Ala Ala Ile Asn Ser Gly Asp Arg Val Asp Ser Ala Leu Lys Asp Met
85 90 95
Ala Val Val Met Lys Gln Leu Asn Arg Ser Asp Glu Ala Ile Glu Ala
100 105 110
Ile Lys Ser Phe Arg His Leu Cys Pro Gln Glu Ser Gln Glu Ser Leu
115 120 125
Asp Asn Val Leu Val Glu Leu Tyr Lys Arg Ser Gly Arg Leu Asp Glu
130 135 140
Gln Ile Glu Met Leu Gln Tyr Lys Leu Lys Asn Ile Asp Glu Gly Ser
145 150 155 160
Ala Phe Gly Gly Lys Arg Thr Lys Ile Ala Arg Ser Gln Gly Lys Lys
165 170 175
Ile Gln Ile Ser Ile Glu Gln Glu Lys Ser Arg Leu Leu Gly Asn Leu
180 185 190
Ala Trp Ala Tyr Leu Gln Gln Gly Asn Tyr Lys Thr Ala Gly Glu Leu
195 200 205
Tyr Lys Gln Ala Leu Ala Leu Asp Pro Asp Arg Asn Lys Glu Cys Asn
210 215 220
Leu Ala Ile Cys Leu Met Tyr Met Asn Lys Ile Lys Glu Ala Lys Ala
225 230 235 240
Met Leu Tyr Ala Ile Gln Val Ser Ser Gln Asn Gly Arg Met Asp Asp
245 250 255
Ser Tyr Val Lys Ser Phe Glu Arg Ala Ser Gln Val Leu Thr Glu Leu
260 265 270
Glu Ala Asn Ser Val Ile Asp Pro Asn Glu Gln Glu Gly His Glu Glu
275 280 285
Met Arg Arg His Leu Arg Ser Leu Val Ser Arg Asn Ser Ile Glu Val
290 295 300
Asn Ser Cys Ile Asn Glu Glu Asn Asp His Leu Ser Gly Leu Val Ala
305 310 315 320
Ser Arg Arg Arg Ala Gly Arg Gln Gln Glu Glu Thr Met Leu Leu Asp
325 330 335
Lys Pro Asn Arg Arg Ser Tyr Cys Gln Asn Gln Phe Glu Asn Lys Asp
340 345 350
Asn Phe Ser Gln Pro Asp Glu Glu Ser Ser Lys Cys Met Ser Leu Gly
355 360 365
Leu Ser Ser Ala Gln Ser Pro Gln Asn Leu Tyr Ala Asp Lys Trp Lys
370 375 380
Lys Gly Ala Gln Leu Glu Asn Pro Phe Glu Arg Ser Asp Phe Ser Ser
385 390 395 400
Arg Arg Lys Gly Asn Trp Val Ser Ala Thr Asp Lys Val Gly Ser Val
405 410 415
Gln Arg Arg Thr Tyr Gly Ser Pro Leu Pro Val Arg Gly Asn Ser Lys
420 425 430
Leu Pro Ser Thr Glu Gln Arg Arg Gly Pro Cys Leu Leu Ser Lys Ala
435 440 445
Asp Gln Arg Lys Ser Thr Trp Gly Glu Asn Thr Ala Asp Ser Pro Gly
450 455 460
Arg Lys Leu Ser Phe Glu Asp Pro Ile Ala Lys Glu Ala Gly Ala Met
465 470 475 480
Ala Pro Gln Asn Pro Asp Gly Arg Leu Gln Ala Ser Ser Asn Glu Lys
485 490 495
Leu Lys Ile Ala Leu Gln Thr Ser Glu Lys Ser Leu Pro Ser Pro Gly
500 505 510
Gly Phe Asp Gly Lys Cys Phe Arg Glu Asn Ser Gly Lys Leu Met Ser
515 520 525
Leu Gln Gln Val Glu Gly Asn Pro Gln Leu Pro Asn Gln Asp Ser Ser
530 535 540
Thr Ser Lys Asn Lys Met Ser Trp Ala Asp Met Leu Asp Tyr Cys Tyr
545 550 555 560
Gln Lys Pro Ser Phe Ser Phe Gln Thr Pro Asn Lys Trp Tyr Asp Gly
565 570 575
Trp Ser His Gly Glu Asp Phe Asn Asp Glu Asn Leu Asn Ser Asn Ile
580 585 590
Phe His Gln Thr Pro Pro Ser Val His Glu Ile Asp Asn Val Ser Tyr
595 600 605
Lys Leu Glu Ala Phe Asp Leu Lys Asp Gly Tyr Asn Thr Pro Gly Ser
610 615 620
Asp Val Ser Ser Arg Asn Asn Pro Thr Ala Arg Arg Ser Leu Ser Asn
625 630 635 640
Glu Thr Met Ser Thr Ser Gly Ser Thr Ile Arg Pro Lys Arg Arg Asn
645 650 655
Arg Leu Gln Val Phe Arg Asp Ile Thr Leu His Thr Glu Ser Pro Arg
660 665 670
Thr
<210> 13
<211> 773
<212> PRT
<213> 黄瓜（Cucumis sativus）
<400> 13
Met Leu Thr Asn Ser Gly Lys Asn Lys Phe Leu Cys Lys Gly Phe Ser
1 5 10 15
Thr Pro Pro Pro Ser Trp Lys Trp Lys Pro Phe Arg Leu Pro Lys Thr
20 25 30
Ala Pro Phe Ser Glu Ser Lys Arg Leu Ser Pro Asn Phe Ala Asn Lys
35 40 45
Ser Asp Leu Phe His Val Ile His Lys Val Pro Ala Gly Asp Ser Pro
50 55 60
Tyr Val Lys Ala Lys Gln Val Gln Leu Ile Asp Lys Asp Pro Asn Arg
65 70 75 80
Ala Val Ser Leu Phe Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Asp
85 90 95
Ser Ala Leu Lys Asp Met Ala Val Val Met Lys Gln Leu Asp Arg Ser
100 105 110
Asp Glu Ala Ile Glu Ala Ile Lys Ser Phe Arg His Leu Cys Pro Tyr
115 120 125
Asp Ser Gln Glu Ser Ile Asp Asn Val Leu Ile Glu Leu Tyr Lys Arg
130 135 140
Ser Gly Arg Ile Glu Glu Glu Ile Asp Met Leu Gln Cys Lys Leu Lys
145 150 155 160
Gln Ile Glu Asp Gly Thr Val Phe Gly Gly Lys Arg Thr Lys Ala Ala
165 170 175
Arg Ser Gln Gly Lys Lys Val Gln Ile Thr Val Glu Gln Glu Lys Ser
180 185 190
Arg Val Leu Gly Asn Leu Ala Trp Ala Phe Leu Gln Leu Asp Asn Ile
195 200 205
Tyr Ile Ala Glu Glu Tyr Tyr Arg Lys Ala Leu Ser Leu Glu Ser Asp
210 215 220
Asn Asn Lys Lys Cys Asn Leu Ala Ile Cys Leu Ile Leu Thr Asn Arg
225 230 235 240
Leu Thr Glu Ala Lys Ser Leu Leu Gln Ser Val Arg Ala Ser Ser Gly
245 250 255
Gly Lys Pro Met Glu Glu Ser Tyr Ala Lys Ser Phe Glu Arg Ala Ser
260 265 270
His Met Leu Ala Glu Lys Glu Ser Lys Ser Phe Asn Ser Thr Glu His
275 280 285
Glu Glu Asp Asn Asn Thr Ala Ala Thr Ile Thr Ser Lys Asn Thr Thr
290 295 300
Gly Lys Ser Gly Arg Cys Val Pro Gln Ile Thr Ala Ser Thr Lys Trp
305 310 315 320
Thr Arg Asp Asp Glu Arg Met Tyr Ile Asn Glu Asn Ser Trp Asp Asp
325 330 335
Asp His His Trp Asp Cys Tyr Glu Asn Lys Ser Ile Gly Ala Val Asn
340 345 350
Ser Ser His Asn Tyr Leu His Cys Asp Lys Trp Ser Glu Gly Cys Phe
355 360 365
Ile Glu Asn Leu Gly Lys Thr Asp Ser Cys Ile Pro Ile Lys Ile Lys
370 375 380
Gly Asp Arg Asn Gln Gly Gly Leu Phe Arg Leu Glu Asp Glu Ser Phe
385 390 395 400
Asn Cys Cys Ser Leu Phe Ser Ser Pro Thr Pro Ala Lys Arg Ser Val
405 410 415
Glu Val Pro Phe Thr Gln Pro Lys Asn Ser Phe Trp Glu Phe Asn Asn
420 425 430
Arg Trp Gly Ser Lys Glu Arg Lys Gln Gln Arg Lys Arg Ile Arg Lys
435 440 445
Val Leu Phe Gly Asn Pro Ser Lys Lys Asn Lys Ser Phe Asp Ser Gly
450 455 460
Phe Leu Val Asp Ser Ser Ser Glu Ser Glu Gly Thr Lys Pro Thr Ser
465 470 475 480
Asn Tyr Lys Thr Lys Tyr Arg Ser Ala Ala Pro Asp Ser Val Glu Leu
485 490 495
Glu Val Pro Phe Thr Gln Pro Arg Ser Cys Glu Trp Val Met Asn Arg
500 505 510
His Ser Arg Lys Ala Thr Glu Cys Phe Arg Ser Leu Arg Ser Ser Ser
515 520 525
Ser Ser Arg Lys Leu Ser Phe Glu Pro Pro Thr Ser Thr Glu Asn Ile
530 535 540
Gln Thr Thr Asn Asp Ser Asn Phe Gly Arg Phe Glu Leu Ser Arg Ala
545 550 555 560
Val Ser Asp Glu Pro Gln Asp Leu Glu Gly Asp Trp Asn Gln Thr Ser
565 570 575
Cys Gly Asp Ile Lys Tyr Glu Glu Gly Gly Ser Pro Met Leu Tyr Gly
580 585 590
Leu Met Lys Lys Ile Lys Glu Glu Cys Ile Ala Val Asp Gln Lys Leu
595 600 605
Gln His Asn Ser Pro Thr Val Phe Gly Lys Lys Ser Trp Ala Asp Met
610 615 620
Val Glu Glu Glu Glu Glu Glu Lys Glu Met Ser Ser Ser Ser Gly Ser
625 630 635 640
Asp Gln Val Asn Cys Phe Ala Asp Asn Trp Ser Cys Ser Ser Asp Asp
645 650 655
Asn Gly Glu Phe Lys Phe Asn Asp Glu Asn Leu Asn Ser Asn Ile Leu
660 665 670
His Gln Asn His Cys Pro Pro Ser Ser Asn Gln Leu Glu Asp Thr Ile
675 680 685
Lys Ile Gly Ser Leu Glu Ile Lys Asp Asp Ser Asp Glu Val Val Ser
690 695 700
Ser Arg Asn Ser Val Glu Arg Cys Pro Leu Tyr Phe Asp Gln Gln Gln
705 710 715 720
Gln Pro Thr Leu Glu Ser Ile Asp Asn Cys Cys Ala Ser Pro Leu Pro
725 730 735
Arg Lys Asp Leu Thr Thr Glu Val Ser Cys Lys Phe Gly Gln Glu Asn
740 745 750
Lys Leu Met Arg Gly Asn Arg Leu Gln Val Phe His Glu Ile Thr Ser
755 760 765
Val His Gln Glu Ser
770
<210> 14
<211> 683
<212> PRT
<213> 黄瓜（Cucumis sativus）
<400> 14
Met Trp Thr Asn Asn Ser Lys Asn Asn Phe Pro Cys Lys Gly Phe Leu
1 5 10 15
Thr Pro Pro Pro Ser Trp Lys Ser Gly Pro Phe Arg Ser Pro Lys Thr
20 25 30
Ala Pro Phe Ser Glu Arg Lys Arg Ser Ser Pro Asn Phe Ala Asn Lys
35 40 45
Ser Asp Leu Phe His Val Ile His Lys Val Pro Ala Gly Asp Ser Pro
50 55 60
Tyr Val Lys Ala Lys Gln Val Gln Leu Ile Glu Lys Asp Pro Ser Arg
65 70 75 80
Ala Val Ser Leu Phe Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Asp
85 90 95
Ser Ala Leu Lys Asp Met Ala Val Val Met Lys Gln Leu Asp Arg Ser
100 105 110
Asp Glu Ala Ile Glu Ala Ile Lys Ser Phe Arg His Leu Cys Pro Tyr
115 120 125
Asp Ser Gln Glu Ser Ile Asp Asn Val Leu Ile Glu Leu Tyr Lys Arg
130 135 140
Ser Gly Arg Ile Glu Glu Glu Ile Asp Met Leu Gln Arg Lys Leu Lys
145 150 155 160
Gln Ile Glu Asp Gly Thr Ile Phe Gly Gly Lys Arg Thr Lys Ala Ala
165 170 175
Arg Ser Gln Gly Lys Lys Val Gln Ile Thr Ile Glu Gln Glu Lys Ser
180 185 190
Arg Val Leu Gly Asn Leu Ala Trp Ala Phe Leu Gln Leu Asn Asn Ile
195 200 205
Tyr Val Ala Glu Asp Tyr Tyr Arg Lys Ala Leu Ser Leu Glu Ala Asp
210 215 220
Asn Asn Lys Lys Cys Asn Leu Ala Ile Cys Gln Ile Leu Thr Asn Arg
225 230 235 240
Leu Thr Glu Ala Lys Ser Leu Leu Gln Ser Val Arg Ala Ser Ser Gly
245 250 255
Gly Lys Pro Met Glu Glu Ser Tyr Ala Lys Ser Phe Glu Arg Ala Phe
260 265 270
His Met Leu Thr Glu Lys Glu Ser Lys Ser Phe Asn Ser Thr Gly Asn
275 280 285
Glu Glu Asp Asn Gly Ala Gly Thr Thr Ile Thr Ser Lys Asn Ala Thr
290 295 300
Gly Arg Thr Gly His Cys Val Pro Gln Ile Ala Ala Ser Thr Arg Trp
305 310 315 320
Thr Arg Asp Asp Glu Gln Met Tyr Ile Asn Glu Asn Ser Arg Asp Ile
325 330 335
Asp Pro His Trp Asp Cys Cys Asp Asp Lys Ser Val Gly Ala Val Asn
340 345 350
Ser Ser His Asn Tyr Leu His Ser Asp Lys Trp Ile Glu Gly Cys Cys
355 360 365
Ile Glu Asn Leu Gly Lys Thr Val Ser Cys Met Pro Ile Lys Met Lys
370 375 380
Gly Asn Arg Asn Arg Asp Ser Leu Phe Arg Leu Val Glu Glu Ser Phe
385 390 395 400
Asn Cys Cys Ser Leu His Thr Ser Pro Thr Pro Thr Lys Lys Asn Val
405 410 415
Glu Val Pro Phe Thr Gln Gln Lys Asn Ser Phe Trp Glu Phe Asn Thr
420 425 430
Arg Trp Arg Ser Lys Glu Arg Lys Gln Gln Gln Lys Arg Thr Arg Lys
435 440 445
Val Leu Phe Glu Asn Pro Ser Arg Lys Asp Gln Ser Phe Asp Ser Gly
450 455 460
Phe Val Val Asp Tyr Ser Ser Glu Ser Asp Glu Thr Glu Pro Ala Ser
465 470 475 480
Asn Tyr Lys Thr Lys Tyr Arg Ser Ala Ala Pro Asp Ser Ile Glu Leu
485 490 495
Glu Val Pro Phe Thr Gln Pro Arg Ser Cys Ser Trp Gly Met Asn Gly
500 505 510
Gly Gly Asn Ser Arg Lys Thr Thr Glu Cys Phe Arg Ser Leu Leu Ser
515 520 525
Arg Ser Ser Ser Arg Lys Leu Ser Phe Glu Leu Pro Thr Ser Thr Glu
530 535 540
Asn Thr Gln Ala Met Thr Asp Ser Asn Leu Gly Arg Ser Lys Leu Ser
545 550 555 560
Arg Glu Ile Ser Asp Glu Pro Gln Asp Leu Ala Gly Gly Asp Trp Lys
565 570 575
Gln Thr Ser Tyr Gly Asp Ile Glu Tyr Glu Glu Gly Thr Ile Pro Asn
580 585 590
Asp Ser Met Lys Ile Met Glu Glu His Met Thr Ile Asp His Lys Phe
595 600 605
Lys His Asn Thr Pro Thr Val Gly Gly Lys Lys Ser Trp Ala Asp Met
610 615 620
Val Glu Glu Glu Glu Glu Asp Ser Asp Asp Lys Asn Glu Asp Asp Thr
625 630 635 640
Glu Glu Thr Leu Ser Ser Ser Gly Arg Gly Gln Val Asn Cys Phe Asp
645 650 655
Asp Asn Trp Ser Ser Ser Ser Asp Asn Val Glu Tyr Lys Phe Asn Asp
660 665 670
Glu Thr Leu Thr Val His Gln Glu Leu Glu Cys
675 680
<210> 15
<211> 644
<212> PRT
<213> 桉树（Eucalyptus grandis）
<400> 15
Met Trp Gly Asn Arg Glu Asn Phe Pro Ala Arg Gly Tyr Phe Thr Pro
1 5 10 15
Gln Pro Pro Lys Arg Ala Val Arg Pro Leu Val Ser Pro Val Leu Met
20 25 30
Ser Glu Arg Lys Arg Ala Ser Pro Pro Asp Leu Phe His Ile Ile His
35 40 45
Lys Val Pro Ala Gly Asp Ser Pro Tyr Val Lys Ala Lys Arg Val Gln
50 55 60
Leu Ile Asp Lys Asp Pro Ser Lys Ala Ile Ser Leu Phe Trp Ala Ala
65 70 75 80
Ile Asn Ala Gly Asp Arg Val Asp Ser Ala Leu Lys Asp Met Ala Ile
85 90 95
Val Met Lys Gln Leu Asn Arg Ser Asp Glu Ala Ile Glu Ala Ile Lys
100 105 110
Ser Phe Arg His Leu Cys Pro Phe Asp Ser Gln Glu Ser Leu Asp Asn
115 120 125
Val Leu Ile Glu Leu Tyr Lys Arg Ser Gly Arg Ile Glu Glu Glu Ile
130 135 140
Glu Met Leu Gln His Lys Leu Arg Leu Ile Glu Glu Gly Lys Gly Phe
145 150 155 160
Val Ala Asn Arg Thr Lys Thr Ala Arg Ser Gln Gly Lys Lys Ile Gln
165 170 175
Val Thr Arg Glu Gln Glu Arg Ser Arg Ile Met Gly Asn Leu Ala Trp
180 185 190
Ala His Leu Gln Leu Ser Asn Tyr Glu Ile Ala Glu Gly Leu Tyr Arg
195 200 205
Glu Ala Leu Ser Leu Glu Pro Asp Lys Asn Lys Gln Cys Asn Leu Ala
210 215 220
Ile Cys Leu Met Asn Met Asn Lys Leu Ala Asp Ala Lys Ala Val Leu
225 230 235 240
Asp Ala Val Arg Gly Pro Cys Gly Asp Gly Asp Met Asp Glu Ser Tyr
245 250 255
Ala Lys Ser Phe Asp Arg Ala Leu Gln Met Leu Asn Glu Ile Lys Ser
260 265 270
Arg Pro Ile Lys Gln Asn Glu Val Asp His Lys Glu Ile Gln Asn Ser
275 280 285
Arg Glu Ser Thr Cys Phe Thr Glu Gln Val Ile Asn Arg Gly Ala Arg
290 295 300
Pro Leu Ala Ser Thr Thr Thr Ala Asn Gly His Gly Glu Glu Thr Trp
305 310 315 320
Ile Leu Asn Glu Arg Asn Gly Met Leu Ser Arg Ala Glu Ala Trp Ser
325 330 335
Ser Ser Glu Asn Ile Phe Gly Asp Asn Trp Arg Glu Val Ser Asn Phe
340 345 350
Gly Thr Pro Leu Arg Tyr Val Leu Pro Gly Asn Leu His Ala Lys Glu
355 360 365
Asn Cys Leu Glu Thr Ser Lys Val Gly Trp Ser Ser Ser Ser Lys Ser
370 375 380
Ala Tyr Val Ser Pro Ala Ser Ile Lys Arg Asn Ile Glu Phe Ser Pro
385 390 395 400
Gly Glu Ser Arg Arg His Thr Cys Arg Ser Leu Tyr Ala Ser Pro Ala
405 410 415
Ser Cys Arg Asp Ser Glu Tyr Lys Ser Lys Ala Thr Ser Thr Gly Glu
420 425 430
Thr Lys Leu Val Gly Asn Gly Ser Val Ala Met Ala Lys Pro Lys Asn
435 440 445
Tyr Asp Glu Asn Met Ile Arg Ser Lys Gly Asp Pro Ala Lys Leu Val
450 455 460
Glu Thr Ser Thr Asp Asp Ile Lys Asp Ser Gly Gly Glu Ser Phe Trp
465 470 475 480
Val Asp Lys His Lys Lys Ser Trp Ala Asp Met Ala Glu Glu Glu Glu
485 490 495
Glu Gln Glu Pro Thr Asp Pro Pro Val Gln His Leu Trp Asn Asp Ser
500 505 510
Pro Gln Lys Pro Pro Pro Phe Arg Thr Pro Thr Arg Ser Phe Arg Ser
515 520 525
Glu Glu Phe Asn Asp Glu Asn Leu Asp Ile Asn Ile Gly Gly His Glu
530 535 540
Arg Thr Leu Thr Glu Asn Leu Ser Arg Thr Leu Thr Phe Ser Asp Leu
545 550 555 560
Gly Cys Gly Ser Lys Ser Gln Thr Met Asp Thr Pro Ser Arg Thr Ser
565 570 575
Gly Arg Ser Ala Val Arg Arg Ser Leu Asn Phe Asn Gln Ile Pro Lys
580 585 590
Gln Glu Leu Asp Ser Val Met Leu Leu Glu Pro Glu Lys Ala Gly Gly
595 600 605
Cys Gly Ala Pro Glu Asp Ala Val Pro Val Lys Lys Ala Gly Gln Pro
610 615 620
Pro Arg Arg Lys Arg Leu Gln Val Phe Gln Asp Ile Thr Arg Leu Pro
625 630 635 640
Pro His Thr Ala
<210> 16
<211> 634
<212> PRT
<213> 桉树（Eucalyptus grandis）
<400> 16
Met Trp Gly Asn Arg Glu Asn Phe Pro Ala Arg Gly Tyr Phe Thr Pro
1 5 10 15
Gln Pro Pro Lys Arg Ala Thr Arg Pro Leu Val Ser Pro Val Leu Met
20 25 30
Ser Glu Arg Lys Arg Ala Ser Pro Pro Asp Leu Phe His Ile Ile His
35 40 45
Lys Val Pro Ala Gly Asp Ser Pro Tyr Val Lys Ala Lys Arg Val Gln
50 55 60
Leu Ile Asp Lys Asp Pro Ser Lys Ala Ile Ser Leu Phe Trp Ala Ala
65 70 75 80
Ile Asn Ala Gly Asp Arg Val Asp Ser Ala Leu Lys Asp Met Ala Ile
85 90 95
Val Met Lys Gln Leu Asn Arg Ser Asp Glu Ala Ile Glu Ala Ile Lys
100 105 110
Ser Phe Arg His Leu Cys Pro Phe Asp Ser Gln Glu Ser Leu Asp Asn
115 120 125
Val Leu Ile Glu Leu Tyr Lys Arg Ser Gly Arg Ile Glu Glu Glu Ile
130 135 140
Glu Met Leu Gln His Lys Leu Arg Leu Ile Glu Glu Gly Lys Gly Phe
145 150 155 160
Val Ala Asn Arg Thr Lys Thr Ala Arg Ser Gln Gly Lys Lys Ile Gln
165 170 175
Val Thr Arg Glu Gln Glu Arg Ser Arg Ile Met Gly Asn Leu Ala Trp
180 185 190
Ala His Leu Gln Leu Ser Asn Tyr Glu Ile Ala Glu Gly Leu Tyr Arg
195 200 205
Glu Ala Leu Ser Leu Glu Pro Asp Lys Asn Lys Gln Cys Asn Leu Ala
210 215 220
Ile Cys Leu Met Asn Met Asn Lys Leu Ala Asp Ala Lys Ala Val Leu
225 230 235 240
Asp Ala Val Arg Gly Pro Cys Gly Asp Gly Asp Met Asp Glu Ser Tyr
245 250 255
Ala Lys Ser Phe Asp Arg Ala Leu Gln Met Leu Asn Glu Ile Lys Ser
260 265 270
Leu Pro Ile Lys Gln Asn Glu Val Asp His Lys Glu Ile Gln Asn Ser
275 280 285
Arg Glu Ser Thr Cys Cys Thr Glu Arg Val Ile Asn Leu Gly Ala Gln
290 295 300
Pro Leu Ala Ser Thr Thr Ile Ala Asn Gly His Gly Glu Glu Thr Trp
305 310 315 320
Ile Leu Asn Glu Arg Asn Gly Met Leu Ser Arg Ala Glu Ala Trp Ser
325 330 335
Ser Ser Glu Asn Ile Phe Gly Asp Asn Trp Arg Glu Val Ser Asn Phe
340 345 350
Gly Thr Pro Leu Arg Tyr Val Leu Pro Gly Asn Leu His Ala Lys Glu
355 360 365
Asn Cys His Glu Thr Ser Lys Val Gly Trp Ser Ser Ser Ser Arg Ser
370 375 380
Ala Tyr Ala Ser Pro Ala Ser Val Lys Arg Asn Ile Glu Phe Ser Pro
385 390 395 400
Gly Glu Ser Arg Arg His Thr Cys Arg Ser Leu Ile Gln Val Glu Gly
405 410 415
Tyr Phe Asn Trp Glu Arg Gln Asn Ser Ser Glu Met Gly Ser Val Ala
420 425 430
Met Ala Lys Pro Lys Asn Tyr Asp Glu Asn Met Ile Arg Ser Lys Gly
435 440 445
Asp Pro Ala Lys Val Val Glu Thr Ser Thr Asp Asp Ile Lys Asp Ser
450 455 460
Gly Gly Glu Ser Phe Trp Val Asp Lys His Lys Lys Ser Trp Ala Asp
465 470 475 480
Met Ala Glu Glu Glu Glu Glu Gln Glu Pro Thr Asp Pro Pro Val Gln
485 490 495
His Leu Trp Asn Asp Ser Pro Gln Lys Pro Pro Pro Phe Arg Thr Pro
500 505 510
Thr Arg Ser Phe Arg Ser Glu Glu Phe Asn Asp Glu Asn Leu Asp Ile
515 520 525
Asn Ile Gly Gly His Glu Arg Thr Leu Thr Glu Asn Leu Ser Arg Thr
530 535 540
Leu Thr Phe Ser Asp Leu Gly Cys Gly Ser Lys Ser Gln Thr Met Asp
545 550 555 560
Thr Pro Ser Arg Thr Ser Gly Arg Ser Ala Val Arg Arg Ser Leu Asn
565 570 575
Phe Asn Gln Ile Pro Lys Gln Glu Leu Asp Ser Val Met Leu Leu Glu
580 585 590
Pro Glu Lys Ala Gly Gly Cys Gly Ala Pro Glu Asp Ala Val Pro Val
595 600 605
Lys Lys Ala Gly Gln Pro Pro Arg Arg Lys Arg Leu Gln Val Phe Gln
610 615 620
Asp Ile Thr Arg Leu Pro Pro His Thr Thr
625 630
<210> 17
<211> 689
<212> PRT
<213> 蓝花耧斗菜（Aquilegia caerula）
<400> 17
Met Gly Ile Asp Asn Lys Lys Val Gly Met Lys Gly Phe Ser Thr Pro
1 5 10 15
Pro Pro Pro Pro Leu Arg Trp Lys Ser Ala Pro Cys Thr Pro Ile Gln
20 25 30
Thr Leu Glu Met Asn Glu Glu Ser Leu His Gln Leu Ser Ala Thr Ile
35 40 45
Ala Lys Val Asp Ser Phe His Ile Ile His Lys Val Pro Ser Gly Asp
50 55 60
Ser Pro Tyr Val Lys Ala Lys His Val Gln Leu Ile Asp Lys Asp Pro
65 70 75 80
Ser Lys Ser Val Ala Leu Phe Trp Ser Ala Ile Asn Ala Gly Asp Arg
85 90 95
Val Asp Ser Ala Leu Lys Asp Met Ala Ile Val Met Lys Gln Leu Asp
100 105 110
Arg Ser Asp Glu Ala Ile Glu Ala Ile Lys Ser Phe Arg His Leu Cys
115 120 125
Thr Pro Leu Ser Gln Glu Ser Leu Asp Asn Val Leu Val Asp Leu Tyr
130 135 140
Lys Arg Gly Gly Arg Ile Glu Glu His Ile Glu Leu Leu His His Lys
145 150 155 160
Leu Lys Leu Ile Asp Asp Gly Val Ala Phe Gly Gly Lys Lys Thr Lys
165 170 175
Ile Ala Arg Ser Gln Gly Lys Lys Phe Leu Val Ser Ile Asp Gln Glu
180 185 190
Arg Ser Arg Leu Leu Gly Asn Leu Gly Trp Ala Tyr Met Gln Gln Asn
195 200 205
Asp Tyr Lys Thr Ala Glu Glu Ile Tyr Arg Lys Ala Leu Ser Ile Glu
210 215 220
Gln Asp Lys Asn Lys Gln Cys Asn Leu Ala Ile Cys Leu Met Asn Arg
225 230 235 240
Gly Glu Ile Met Glu Ala Lys Ser Leu Leu Gln Thr Val Thr Pro Ser
245 250 255
Ser Thr Glu Arg Glu Leu Val Asp Pro Phe Ile Lys Ser Phe Asp Arg
260 265 270
Ala Tyr Glu Met Leu Ile Glu Leu Glu Ser Lys Ser Cys Pro Asn Arg
275 280 285
Asn Ala Lys Pro Val Ala Ser Ala Ser Pro Ile Glu Glu Pro Leu Leu
290 295 300
Leu Lys Lys Gly Ser Tyr Val Cys Asp Asp Arg Leu Asp Ser Phe Gly
305 310 315 320
Lys Gly Arg Leu Asn Arg Glu Val Glu Met Glu Glu Lys Gly Gly Gly
325 330 335
His Ser Cys Arg Lys Ser Leu Phe Ala Asp Lys Gly Asn Lys Glu Asn
340 345 350
Cys Ser Pro Asn Leu Phe Glu Arg Gly Asn Gly Ile Ser Gly Trp Lys
355 360 365
Asn Val Glu Pro Phe Ser Glu Ser Arg Met Glu Glu Gly Ser Thr Arg
370 375 380
Lys Asn Val Arg Thr Phe Gly Phe His Arg Tyr Ser Asn Arg Ser Pro
385 390 395 400
Lys Leu Thr Thr Lys Tyr Gly Leu Gln Ser Pro Ser Asp Gly Asp Trp
405 410 415
Arg Arg Arg Ser Arg Asp Asn Asp Ala Asn Lys Asn Gln Ala Thr Ile
420 425 430
Thr Pro Thr Lys Ser Pro Ile Phe Ile Glu Ser Lys Cys Gly Leu Gln
435 440 445
Ser Thr Asp Gly Gly Cys Arg Lys Ser Pro Ile Ser Phe Ala Tyr Gln
450 455 460
Lys Ala His Glu Val Gly Gly Asp Leu Gln Ala Cys Ser Gly Gln His
465 470 475 480
Leu Glu Gln Arg Ser Glu Val Thr Val Thr Asp Ser Ala Ser Lys Tyr
485 490 495
Ile Gly Gly Asn Glu Thr Pro Glu Ala Cys Asn Gly Ile Asp Ser Thr
500 505 510
Thr Glu Gly Ser Thr Ser Lys Tyr Leu Cys Ser Ser Phe Arg Ser Gly
515 520 525
Lys Ser Trp Ala Asp Met Ala Glu Glu Glu Glu Glu Leu Leu Arg Glu
530 535 540
Cys Lys Asn Asn Ser Ile Ser Thr Trp Glu Tyr Pro Ser Phe Gln Thr
545 550 555 560
Pro Cys Lys Ser Val Asp Arg Trp Asn Asp Glu Lys Phe Phe His Asn
565 570 575
Glu Asn Met Asp Ser Asn Ile Ile Gly Ser Thr Pro Glu Leu Thr Thr
580 585 590
Gln Pro Arg Arg Asp Gly Tyr Thr Gln Thr Leu Ile Arg Lys Leu Glu
595 600 605
Leu Ile Asp Leu Lys Lys Glu Glu His Ile Lys Thr Thr Ala Ser Tyr
610 615 620
Ser Phe Ser Asn Pro Thr Ala Arg Arg Thr Leu Ala Phe Glu Lys Thr
625 630 635 640
Asp Ser Ala Asp Tyr Phe Arg Pro Ser Pro Leu Cys Lys Gln Val Leu
645 650 655
Phe Asn Gly Glu Ser Asp Asn Thr Ala Gly His Gly Lys Asn Ser Met
660 665 670
Pro Val Gly Arg Arg Ser Arg Arg Leu Pro Val Phe Gln Glu Ile Thr
675 680 685
Pro
<210> 18
<211> 625
<212> PRT
<213> 菜豆（Phaseolus vulgaris）
<400> 18
Met Leu Phe Glu Arg Gly Ser Pro Ala Arg Cys Phe Val Thr Pro Pro
1 5 10 15
Pro Pro Ser Pro Trp Lys Thr Ile Ser Ser Arg Ser Pro Ser Val Pro
20 25 30
Phe Ser Glu Arg Lys Lys Ser Pro Asn Ser Ala Asn Lys Ser Asp Ile
35 40 45
Phe His Ile Ile His Lys Val Pro Ser Gly Asp Ser Pro Tyr Val Lys
50 55 60
Ala Lys Gln Val Gln Leu Val Asp Lys Asp Pro Gly Arg Ala Ile Ser
65 70 75 80
Leu Phe Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Glu Ser Ala Leu
85 90 95
Lys Asp Met Ala Leu Val Met Lys Gln Leu Asn Arg Ser Asp Glu Ala
100 105 110
Ile Glu Ala Ile Arg Ser Phe Arg His Leu Cys Pro Ser Asp Ser Gln
115 120 125
Asp Ser Leu Asp Asn Ile Leu Val Glu Leu Phe Lys Arg Ser Gly Arg
130 135 140
Val Asp Glu Glu Ile Ser Met Leu His His Lys Leu Lys Leu Ile Glu
145 150 155 160
Asp Gly Ile Thr Phe Val Gly Arg Thr Thr Lys Gln Ala Arg Ser Gln
165 170 175
Gly Lys Lys Ile Gln Ile Thr Ala Glu Gln Glu Ile Ser Arg Ile Leu
180 185 190
Gly Asn Leu Ala Trp Ala Tyr Leu Gln Lys Glu Asp Tyr Lys Thr Ala
195 200 205
Glu Glu His Tyr Arg Arg Ala Leu Ser Phe Glu Val Asp Arg Asn Lys
210 215 220
Gln Cys Asn Leu Ala Ile Cys Leu Met His Met Asn Lys Ile Lys Glu
225 230 235 240
Ala Lys Phe Leu Leu Gln Ala Val Arg Thr Ala Thr Lys Asn Arg Lys
245 250 255
Met Asp Glu Ser Phe Val Lys Ser Phe Glu Arg Ala Ser Gln Met Leu
260 265 270
Val Glu Ile Glu Thr Ser Ser Ser Glu Asn Ala Ser Phe Ser Met Thr
275 280 285
Thr Ser Leu Pro Gln Cys Ser Ser Leu Gly Phe Glu Asn Ser Val Arg
290 295 300
Lys Ser Ser Asp Arg Val Lys Arg Thr Glu Asn Gln Ser Asp Lys Ser
305 310 315 320
Glu Gly Asp Val Ser His Ala Arg Arg Arg Leu Tyr Gln Ser Pro Asp
325 330 335
Pro Gly Arg Arg His Leu Asn Leu Tyr Val Pro Thr Lys Pro Lys Arg
340 345 350
Cys Ser Trp Gly Phe Asn Asn Gly Tyr Arg Arg Gly Asp Phe His Ser
355 360 365
Asp Ser Lys Pro Ser Ser Gly Thr Pro Pro Asn Glu Lys His Val Thr
370 375 380
Arg Thr Leu Asn Leu Arg Glu Ser Gly Leu Ser Ser Pro Ala Asn Glu
385 390 395 400
Lys Trp Arg Thr Ser Thr Leu Glu Asp Pro Ala Ile Arg Lys Asn Lys
405 410 415
Asp Thr Lys Val Ile Ile Ser Asp Leu Leu His Thr Leu Asn Thr Glu
420 425 430
Ala Val Val Glu Phe Thr Glu Lys Glu Lys Ser Ala Ala Gly Asp Ser
435 440 445
Ser His Arg Ser Ile Met Ser Glu Ser His Ala Met Val Glu Asn Gly
450 455 460
Thr Asp Asp Phe Ala Ser Gly Asn Gly Lys Pro His Glu Lys Lys Ser
465 470 475 480
Trp Ala Asp Ile Val Glu Glu Glu Gln Asn Glu Glu Asn Asp Phe Phe
485 490 495
Ser Gly Tyr Ile Asn Phe Asp Gly Glu Asn Gly Ala Glu Val Phe Asn
500 505 510
Asp Glu Asn Glu Asp Ser Asn Ile Met Tyr Gln Ser Pro Trp Pro Gln
515 520 525
Asn Gln Pro Glu Trp Ser Ser Lys Lys Leu Glu Ser Leu Glu Gln Lys
530 535 540
Asp Gly Tyr His Pro Ser Gly Ser Val Ile Leu Ser Arg Asn Pro Thr
545 550 555 560
Ala Arg Arg Ser Leu Cys Phe Asn Pro Glu Leu Ser Ser Glu Ser Ala
565 570 575
Tyr Ala Ile Arg Thr Ser Lys Ser Pro Lys Lys Ala Ser Asn Leu Glu
580 585 590
Asn Arg Asp Thr Leu Val Gly Glu Lys Lys Leu Pro Arg Lys Ser Arg
595 600 605
Leu Gln Val Phe Gln Asp Ile Thr Leu Phe Pro Glu Thr Pro Arg Phe
610 615 620
Ala
625
<210> 19
<211> 758
<212> PRT
<213> 柿树（Prunus persica）
<400> 19
Met Trp Thr Arg Asp Lys Ser Leu His Thr Arg Gly Phe Ser Thr Pro
1 5 10 15
Pro Pro Thr Trp Lys Ser Gly Pro Pro Asn Pro Pro Met Val Pro Met
20 25 30
Ser Glu Arg Lys Arg Val Ser Pro Ser Asp Gly Gly Asp Leu Phe His
35 40 45
Val Met His Lys Val Pro Val Gly Asp Ser Pro Tyr Val Arg Ala Lys
50 55 60
Gln Val Gln Leu Ile Glu Lys Asp Pro Ser Lys Ala Ile Ser Leu Phe
65 70 75 80
Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Asp Ser Ala Leu Lys Asp
85 90 95
Met Ala Ile Val Met Lys Gln Leu Asn Arg Ser Glu Glu Ala Ile Glu
100 105 110
Ala Ile Lys Ser Phe Arg His Leu Cys Pro His Asp Ser Gln Glu Ser
115 120 125
Leu Asn Asn Val Leu Val Glu Leu Tyr Lys Arg Ala Gly Arg Ile Glu
130 135 140
Glu Glu Ile Glu Met Leu Gln Ser Lys Leu Lys His Ile Asp Glu Gly
145 150 155 160
Ile Ala Phe Gly Gly Arg Arg Thr Lys Thr Ala Arg Ser Gln Gly Lys
165 170 175
Lys Val Gln Ile Thr Val Glu Gln Glu Arg Ser Arg Ile Leu Gly Asn
180 185 190
Leu Ala Trp Ala Tyr Leu Gln Gln Gly Asn Tyr Lys Thr Ala Glu Glu
195 200 205
Tyr Tyr Met Lys Ser Leu Ser Leu Glu Leu Asp Lys Asn Lys Gln Cys
210 215 220
Asn Leu Ala Ile Cys Leu Met His Met Asn Arg Leu Ala Glu Ala Lys
225 230 235 240
Ser Leu Leu Gln Val Val Arg Ala Ser Ser Gly Asn Lys Pro Met Asp
245 250 255
Glu Ser Tyr Ala Lys Ser Phe Glu Arg Ala Ile Gln Met Leu Thr Glu
260 265 270
Leu Glu Ala Lys Ser Val Leu Arg Pro Ile Gln Leu Asp Glu Asn Cys
275 280 285
Cys Lys Glu Ile Ser Arg Phe Pro Ile Ser Pro Ile Asn Arg Asn Ser
290 295 300
Lys Gln Gly Asn Ser Leu Thr Asn Glu Gly Gln His Tyr Val Ser Gly
305 310 315 320
Cys Met Ile Ser Arg Arg Trp Ala Asp Gly His Glu Glu Glu Thr Val
325 330 335
Ser Val Asn Lys Trp Lys Lys Asp Cys Tyr Phe Lys Asn Ser Cys Glu
340 345 350
Gly Arg Ser Ser Phe Ser Ser Arg Met Lys Glu Asn Gln Gly Gly Ile
355 360 365
Val Gly Thr Glu Thr Thr Pro Tyr Ser Lys Thr Phe Phe Ser Pro Ala
370 375 380
Pro Asp Ile Trp Asn Arg Glu Val Leu Phe Thr Gln Pro Arg Arg Ser
385 390 395 400
Ser Arg Gly Phe Asn Asp Gly His Gln Thr Arg Glu Ile Trp Gly Arg
405 410 415
Gly Val Gly Ser Ser Asn Lys Lys Leu Ser Phe Glu Ser Cys Ser Arg
420 425 430
Thr Glu Asn Met Arg Ala His Val Val Arg Ser Leu Asn Glu Asp Leu
435 440 445
Leu Ala Ser Thr Thr Gly Lys Ser Glu Val Ala Phe Gln Asn Ser Val
450 455 460
Ser Ser Ile Ser Ser Pro Ile Ser Arg Asp Leu Arg Arg Arg Pro Gln
465 470 475 480
Lys Asp Ala Ala Val Arg Ser Val Leu Gln Pro Ile Ser Ser Gly Asn
485 490 495
Trp Lys Cys Thr Ser Arg Ala Asn Asp Gly Cys Phe Gln Leu Lys Asp
500 505 510
Glu Ala Val Val Val Ser Ser Ser Gln Asn Thr Val Asn Gly Asp Trp
515 520 525
Arg Arg Thr Ser Trp Glu Asn Asp Gly Met Lys Lys Ser Ala Glu Pro
530 535 540
Leu Met Val Gly Glu Asp Ala Lys Ala Leu Glu Ile Ser Thr Asp Gly
545 550 555 560
Gly Pro Asn Gln Ser Ser Asp Thr Thr Ala Phe Val Glu Asp Cys Phe
565 570 575
Gly Glu Asn Thr Ser Ser Lys Val Asp Asp Val His Gln Pro Ile Ala
580 585 590
Glu Asn Gln Lys Pro Ala Pro Asp Phe Ser Met Tyr Ser Lys Cys Lys
595 600 605
Lys Ser Trp Ala Asp Met Val Glu Glu Glu Glu Gln Glu Leu Leu Asn
610 615 620
Gly Arg Thr Glu Tyr Phe Asp Ser Trp Tyr Thr Glu Asp Gly Phe Asn
625 630 635 640
Asn Glu Asn Leu Asn Cys Asn Ile Thr Pro Glu Ser Pro Cys Leu Gln
645 650 655
Ser Gln Met Lys Ser Leu Gly Gln Lys Leu Gln Ser Thr Asp Leu Val
660 665 670
Asp Glu Tyr Val Ser Gly Asn Ala Ala Ser Ser Arg Asn Ser Thr Val
675 680 685
Arg Arg Ser Leu Cys Phe Gly Gln Gln Gln Glu Gln Glu Ser Val Asp
690 695 700
Tyr Ile Ser Ser Ser Pro Val Pro Lys Glu Ala Leu Asn Phe Glu Gly
705 710 715 720
Ser Asp Ser Val Gln Ala Asn Gly Lys Gly Ser Ile Tyr Gly Lys Asn
725 730 735
Ser Ser Phe Ser Arg Arg Lys Arg Leu Gln Ser Phe Gln Asp Ile Thr
740 745 750
Glu Phe Gln Asp Ser Pro
755
<210> 20
<211> 649
<212> PRT
<213> 雷蒙德氏棉（Gossypium raimondii）
<400> 20
Met Trp Ser Ser Asp Lys His Cys Pro Ala Arg Gly Phe Leu Thr Pro
1 5 10 15
Gln Pro Pro Ala Trp Lys Lys Val Gln Ser Thr Thr Val Leu Pro Met
20 25 30
Ser Glu Arg Lys Arg Ile Ser Pro Ala Asn Asn Gly Asp Cys Phe His
35 40 45
Val Ile His Lys Val Pro Ala Ser Asp Ser Pro Tyr Gly Arg Ala Lys
50 55 60
His Val Gln Leu Ile Asp Lys Asp Pro Ser Lys Ala Val Ser Leu Phe
65 70 75 80
Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Asp Ser Ala Leu Lys Asp
85 90 95
Met Ala Val Val Met Lys Gln Leu Asn Arg Ser Asp Glu Ala Ile Glu
100 105 110
Ala Ile Lys Ser Phe Arg His Leu Cys Pro Tyr Asp Ser Gln Glu Ser
115 120 125
Leu Asp Asn Val Leu Val Glu Leu Tyr Lys Arg Ser Gly Arg Val Asp
130 135 140
Glu Glu Ile Glu Ile Leu Leu Asn Lys Leu Arg Asn Ile Glu Glu Gly
145 150 155 160
Thr Val Phe Gly Gly Lys Lys Thr Lys Ile Ala Arg Ser Gln Gly Lys
165 170 175
Lys Ile Gln Ile Thr Ile Glu Gln Glu Lys Ser Arg Ile Leu Gly Asn
180 185 190
Leu Ala Trp Ala Tyr Leu Gln Gln His Asn Tyr Gly Ile Ala Glu Gln
195 200 205
His Tyr Arg Lys Ala Leu Ser Leu Glu Pro Asp Lys Asn Lys Gln Cys
210 215 220
Asn Leu Ala Ile Cys Leu Met His Met Asn Arg Leu Gly Glu Ala Lys
225 230 235 240
Ser Leu Leu Gln Asp Val Lys Val Ser Ala Gly Thr Glu Glu Met Asp
245 250 255
Glu Ser Tyr Ser Lys Ser Tyr Glu Arg Ala Met Glu Ile Leu Met Gln
260 265 270
Val Glu Thr Gln Ser Lys Leu Glu Pro Ala Gly Ala Gln Glu Pro Glu
275 280 285
Lys Gly Asn Glu Thr Arg Arg Cys Leu Thr Ser Cys Arg Asp Arg Ser
290 295 300
Leu Lys Glu Ala Ser Val Phe Leu Pro Arg Asn Gly Asp Asn Ile Pro
305 310 315 320
Trp Cys Ile Glu Lys Asn Gly Asn Leu Ser Gly Tyr Asp Asp Thr Ser
325 330 335
Ser Ser Gln Cys Thr Pro Ile Gly Leu Lys Gly Ser Phe Gln Cys Ser
340 345 350
Pro Gln Thr Met Leu Ser Glu Lys Trp Arg Lys Gly Ser Tyr Phe Glu
355 360 365
Ser Pro Ser Glu Gly Ser Val Tyr Ser Ser Ser Lys Leu Lys Glu Ser
370 375 380
Trp Arg Tyr Ser Ala Gly Gln Glu Val Gly Ser Ala His Lys Asn Met
385 390 395 400
Tyr Ala Ser Leu Ala Ala Ser Arg Lys Asn Ser Glu Lys Val Leu Leu
405 410 415
Thr Gln Pro Arg Arg Cys Ser Trp Gly Phe Asn Thr Ala Asp Gln Arg
420 425 430
Arg Gly Gly Arg Trp Gly Glu Asp Thr Thr Val Arg Asn Ser Ile Arg
435 440 445
Lys Leu Ser Phe Glu Gln Thr Thr Thr Thr Glu Ser Val Pro Ser Pro
450 455 460
Ser Ile Gln Lys Leu Lys Glu Glu Pro Leu Ser Ser Ser Asn Ala Lys
465 470 475 480
Ser Glu Asn Tyr Ser Ala Val Gly Leu Gly Glu Glu Glu Ala Gln Glu
485 490 495
Gly Leu Ser Gly Val Leu Phe Thr Gln Pro Arg Asn Ser Leu Ser Trp
500 505 510
Leu Asn Asn Arg Asp Gln Arg Arg Gly Arg Cys Ala Glu Glu Ser Ile
515 520 525
Asp Gly Ser Phe Ser Lys Leu Ser Ser Ser Val Thr Thr His Ser Val
530 535 540
Gln Ser Leu Asn Val Glu Pro Leu Val Ser Ser Lys Asp Glu Ser Glu
545 550 555 560
Ile Gly Val Glu Lys Pro Ala Asp Ala Ala Ser Asn Lys Lys Thr Trp
565 570 575
Ala Asp Met Val Glu Glu Glu Glu Lys Asp Glu Phe Leu Asn Asp Glu
580 585 590
Asn Leu Asn Ser Asn Ile Ile Tyr Gln His Pro Asp Arg Ser Lys His
595 600 605
His Ile Glu Asn Ile Thr Gln Gln Leu Glu Ser Phe Gly Val Lys Gly
610 615 620
Gly Tyr Asn Ala Ser Ala Asn Thr Val Ser Leu Arg Arg Asn Arg Leu
625 630 635 640
Gln Val Phe Arg Asp Ile Thr Ser Thr
645
<210> 21
<211> 614
<212> PRT
<213> 番茄（Solanum lycopersicum）
<400> 21
Met Trp Arg Asn Asn Glu Arg Val Tyr Met Thr Pro Ala Arg Gly Phe
1 5 10 15
Leu Thr Pro Pro Pro Lys Trp Arg Ser Pro Ala Thr Glu Lys Asp Gln
20 25 30
Lys Trp Pro Thr His Ala Gln Ser Ala Lys Ala Asp Leu Phe His Val
35 40 45
Ile His Lys Val Pro Ser Gly Asp Ser Pro Tyr Val Arg Ala Lys His
50 55 60
Val Gln Leu Ile Asp Lys Asp Pro Gly Lys Ala Val Ser Leu Phe Trp
65 70 75 80
Ala Ala Ile Asn Ser Gly Asp Arg Val Asp Ser Ala Leu Lys Asp Met
85 90 95
Ala Val Val Met Lys Gln Leu Asp Arg Ser Asp Glu Ala Ile Glu Ala
100 105 110
Ile Lys Ser Phe Arg Asn Leu Cys Pro Ser Glu Ser Gln Glu Ser Ile
115 120 125
Asp Asn Ile Leu Ile Glu Leu Tyr Lys Arg Ser Gly Arg Leu Glu Glu
130 135 140
Glu Ile Glu Leu Leu Glu Leu Lys Leu Lys Asn Val Glu Glu Gly Ile
145 150 155 160
Ala Phe Gly Gly Lys Arg Thr Lys Ile Ala Arg Ser Gln Gly Lys Lys
165 170 175
Val Gln Ile Thr Ile Glu Lys Glu Tyr Ala Arg Leu Leu Gly Asn Leu
180 185 190
Ala Trp Ser Tyr Met Gln Leu Asn Asn Phe Lys Leu Ala Glu Glu Tyr
195 200 205
Tyr Arg Lys Ala Leu Ser Leu Glu Ser Asp Lys Asn Lys Gln Ser Asn
210 215 220
Leu Ala Ile Cys Leu Met His Met Asn Lys Ile Ala Glu Ala Arg Phe
225 230 235 240
Leu Leu Gln Ser Ile Lys Thr Ser Asp Arg Arg Gln Met Asp Glu Ser
245 250 255
Cys Thr Lys Ser Phe Glu Arg Ala Thr Gln Met Leu Ala Glu Leu Glu
260 265 270
Ser His Gly Ile Gln Asn Ser Lys Glu Gln Val Glu Glu Met Arg Glu
275 280 285
Val Arg Ile Asp Ser Ser Thr Ser Asp Glu His Asp Arg Arg Gly His
290 295 300
Glu Lys Thr His Pro Pro Pro Phe Thr Ala Ser Gly Pro Pro Lys His
305 310 315 320
Phe Leu Thr Gln Pro Arg Arg Tyr Ser Cys Ser Leu Asn Asp Gly Gly
325 330 335
Trp Leu Asn Lys Asp Ser Val Ser Ala Cys Ser Arg Arg Leu Leu Phe
340 345 350
Glu Gln Thr Ser Asn Asn Glu Asn Val Gln Leu Val Val Asn His Asn
355 360 365
Phe Asn Lys Leu Ile Ser Val Asn Asp Ile Ser Glu Gly Ala Ser Val
370 375 380
Val Cys Gly Gln Val Phe Ser Arg Ser Trp Gly Asn Gly Ala Asn Val
385 390 395 400
Arg Ser Glu Cys Asp Leu Gln Pro Pro Tyr Ser Lys Trp Lys Asn Asn
405 410 415
Ser Ser Gly Asn Asp Gly Ser Asp Gln Ile Ser Leu Glu Leu Ser Arg
420 425 430
Ser Pro Thr Glu Ser Leu Pro Asp Ile Thr Ser Ala Arg Lys Cys Ser
435 440 445
Glu Asn Gly Ser Lys Asp Cys Trp Ser Ser Thr Leu Thr Tyr Arg Asp
450 455 460
Met Val Thr Leu Glu Asp Thr Thr Glu His Leu Asp Ser Thr Ser Leu
465 470 475 480
Lys Pro Leu Asn Leu Pro Ala Cys Thr Ser Lys Lys Ser Trp Ala Asp
485 490 495
Met Val Glu Glu Asp Glu Leu Gly Leu Gln Phe His Glu Thr Pro Gly
500 505 510
Lys Tyr Ser Asp Glu Asn Glu Asn Ile Asp Ala Asn Ile Ile Asn Leu
515 520 525
Ser Gln Asn Ile Asp Thr Leu Cys Leu Asn Glu Gly Tyr His Thr Gln
530 535 540
Pro Gly Arg Glu Ala Arg Arg Ser Leu Cys Phe Asp His Asn Asp Arg
545 550 555 560
Lys Glu Lys Cys Ser Ser Gly Phe Gln Gly Lys Glu Leu Lys Ser Gly
565 570 575
Ser Leu Asn Ser Leu Pro Pro Ile Gly Asp Ile Ala Tyr Gln Thr Pro
580 585 590
Val Thr Leu Met Arg Arg Asn Arg Leu Gln Val Phe Arg Asp Ile Thr
595 600 605
Pro Glu Ser Pro Lys Pro
610
<210> 22
<211> 614
<212> PRT
<213> 马铃薯（Solanum tuberosum）
<400> 22
Met Trp Arg Asn Asn Glu Arg Val Tyr Met Ser Pro Ala Arg Gly Phe
1 5 10 15
Leu Thr Pro Pro Pro Lys Trp Arg Ser Pro Ala Ser Asp Lys Asp Gln
20 25 30
Arg Trp Pro Thr His Ala Gln Ser Ala Lys Ala Asp Leu Phe His Val
35 40 45
Ile His Lys Val Pro Ser Gly Asp Ser Pro Tyr Val Arg Ala Lys His
50 55 60
Val Gln Leu Ile Asp Lys Asp Pro Gly Lys Ala Ile Ser Leu Phe Trp
65 70 75 80
Ala Ala Ile Asn Ser Gly Asp Arg Val Asp Ser Ala Leu Lys Asp Met
85 90 95
Ala Val Val Met Lys Gln Leu Asp Arg Ser Asp Glu Ala Ile Glu Ala
100 105 110
Ile Lys Ser Phe Arg Asn Leu Cys Pro Ser Glu Ser Gln Glu Ser Ile
115 120 125
Asp Asn Ile Leu Ile Glu Leu Tyr Lys Arg Ser Gly Arg Leu Glu Glu
130 135 140
Glu Ile Glu Leu Leu Glu Leu Lys Leu Lys Asn Val Glu Glu Gly Ile
145 150 155 160
Ala Phe Gly Gly Lys Arg Thr Lys Ile Ala Arg Ser Gln Gly Lys Lys
165 170 175
Val Gln Ile Thr Ile Glu Lys Glu Tyr Ala Arg Leu Leu Gly Asn Leu
180 185 190
Ala Trp Ser Tyr Met Gln Leu Asn Asn Phe Lys Leu Ala Glu Glu Tyr
195 200 205
Tyr Arg Lys Ala Leu Ser Leu Glu Ser Asp Lys Asn Lys Gln Ser Asn
210 215 220
Leu Ala Ile Cys Leu Met His Met Asn Lys Ile Ala Glu Ala Arg Phe
225 230 235 240
Leu Leu Gln Ser Ile Lys Ala Ser Asp Arg Trp Gln Met Asp Glu Ser
245 250 255
Cys Thr Lys Ser Phe Glu Arg Ala Thr Gln Met Leu Ala Glu Leu Glu
260 265 270
Thr His Gly Ile Gln Asn Ser Lys Glu Gln Val Glu Glu Met Arg Glu
275 280 285
Val Arg Ile Asp Ser Ser Thr Ser Asp Glu His Asp Cys Arg Gly His
290 295 300
Glu Lys Thr His Pro Pro Pro Phe Thr Ala Ser Gly Pro Pro Lys His
305 310 315 320
Phe Leu Thr Gln Pro Arg Arg Tyr Ser Cys Ser Leu Asn Asp Gly Gly
325 330 335
Trp Leu Asn Lys Asp Ser Val Ser Ala Cys Ser Arg Arg Leu Leu Phe
340 345 350
Glu Gln Thr Ser Asn Asn Glu Asn Val Gln Leu Val Val Asn His Asn
355 360 365
Phe Asn Lys Leu Ile Ser Ala Asn Asp Met Ser Glu Gly Ala Ser Leu
370 375 380
Val Arg Gly Gln Val Leu Thr Arg Ser Trp Gly Asn Gly Ala Asn Val
385 390 395 400
Glu Ser Glu Cys Asp Leu Gln Pro Pro Tyr Ser Lys Trp Lys Asn Asn
405 410 415
Ser Ser Gly Asn Asp Gly Ser Asp Gln Ile Ser Leu Glu Leu Ser Lys
420 425 430
Ser Pro Thr Glu Ser Leu Ala Asp Ile Thr Ser Ala Arg Lys Tyr Ser
435 440 445
Gly Asp Gly Ser Lys Asp Cys Trp Ser Ser Thr Leu Thr Tyr Arg Asp
450 455 460
Thr Val Thr Leu Glu Asp Thr Thr Glu His Leu Glu Ser Thr Asn Leu
465 470 475 480
Lys Pro Leu Asn Leu Pro Ala Cys Thr Ser Lys Lys Ser Trp Ala Asp
485 490 495
Met Val Glu Glu Asp Glu Leu Gly Leu Gln Phe His Glu Thr Pro Gly
500 505 510
Lys Tyr Ser Asp Glu Asn Glu Asn Ile Asp Ser Asn Ile Ile Asn Leu
515 520 525
Ser Gln Asn Ile Asp Thr Leu Arg Leu Asn Glu Gly Tyr His Thr Gln
530 535 540
Pro Gly Arg Glu Ala Arg Arg Ser Leu Cys Phe Asp Gln Asn Asp Arg
545 550 555 560
Lys Glu Lys Cys Ser Ser Asp Phe Gln Gly Lys Val Leu Lys Ser Gly
565 570 575
Ser Leu Asn Ser Leu Pro Pro Ile Gly Asp Ile Ala Tyr Gln Thr Pro
580 585 590
Val Thr Leu Met Arg Arg Asn Arg Leu Gln Val Phe Arg Asp Ile Thr
595 600 605
Pro Glu Ser Pro Lys Pro
610
<210> 23
<211> 683
<212> PRT
<213> 粟（Setaria italica）
<400> 23
Met Pro Ser Gly Gly Arg Arg Leu Pro Pro Trp Thr Ser Pro Arg Ser
1 5 10 15
Ala Gly Ala Pro Arg Trp Ser Pro Ala Ala Gly Thr Pro Val Ala Gly
20 25 30
Ala Gly Cys Gly Pro Val Ser Gly Tyr Arg Thr Pro Pro Val Ser Ala
35 40 45
Gly Gly Cys Phe Gly Thr Arg Val Thr Pro Pro Thr Ser Gly Gly Ala
50 55 60
Arg Val Thr Pro Pro Ser Thr Gly Gly Cys Ser Ser Arg Pro Pro Arg
65 70 75 80
Pro Pro Pro Ser Leu Asp Ser Pro Tyr Val Arg Ala Lys Gln Ala Gln
85 90 95
Val Ile Glu Lys Asp Pro Asn Lys Ala Val Pro Leu Phe Trp Ala Ala
100 105 110
Ile Asn Ser Gly Asp Arg Ile Glu Ser Ala Leu Lys Asp Met Ala Asn
115 120 125
Val Leu Lys Gln Ala Asn Arg Ala Glu Glu Ala Ile Glu Ala Ile Arg
130 135 140
Ser Phe Arg Asp Arg Cys Pro Tyr Glu Ala Gln Asp Ser Leu Asp Asn
145 150 155 160
Ile Leu Leu Asp Leu Tyr Lys Lys Cys Gly Arg Thr Glu Glu Gln Ile
165 170 175
Glu Met Leu Thr Ile Lys Leu Arg Val Val Asp Glu Glu Leu Ala Ser
180 185 190
Gly Arg Trp Lys Thr Lys Leu Ser Lys Ser His Gly Arg Val Val Tyr
195 200 205
Leu Ser Leu Arg Asp Glu Lys Ala Arg Leu Leu Gly Asn Leu Ala Trp
210 215 220
Ala Tyr Met Gln Ser Glu Asn Tyr Glu Glu Ala Glu Met Leu Tyr Arg
225 230 235 240
Gln Ala Leu Ala Ile Glu Ala Asp Tyr Asn Lys Glu Cys Asn Leu Ala
245 250 255
Ile Cys Leu Met Lys Thr Gly Lys Leu Ala Glu Ala Lys Tyr Leu Leu
260 265 270
Gln Ala Ile Pro Tyr Asn Cys Asp Asp Glu Ser His Val Lys Ser Leu
275 280 285
Ser Arg Ala Thr Glu Met Leu Arg Asp Leu Glu Leu Gln Ser Leu Pro
290 295 300
Ser Pro Ile Thr Gln Met Lys Ser Lys Glu Ser Arg Ile Leu Leu Ala
305 310 315 320
Thr Asp Val Glu Ile Leu Glu Asp Pro Gln Pro Gln Thr Leu Ser Thr
325 330 335
Pro Leu Ser Gln Leu Lys Tyr Lys Glu Pro His Ile Ser Val Ser Ala
340 345 350
Asn Ala Glu Gln His Glu Lys Cys Ser Ser Trp Phe Pro Ser Pro Ile
355 360 365
Thr Gln Leu Lys Arg Glu Glu Pro Arg Ile Leu Val Thr Val Asp Ala
370 375 380
Glu Lys Asn Glu Gly Cys Ala Glu Phe Gln Asp Leu Ser Arg Leu Phe
385 390 395 400
Asn Asp Ala Ala Thr Pro His Ser Ile Leu Glu Lys Leu Arg Lys Arg
405 410 415
Leu Val Asn Glu Ala Pro Lys Ser Ser Ile His Asp Gln Ile Gln Thr
420 425 430
His Thr Pro Thr Glu Cys Leu Pro Asn Ser Glu Gly Asn His Asn Ala
435 440 445
Ser Glu Asn Pro Val Gln Gly Gly Lys Leu Leu Thr Lys Gly Val Arg
450 455 460
Lys Thr Trp Ala Asp Met Val Asp Glu Glu Glu Gln Gln Leu Gly Glu
465 470 475 480
Asp Lys Ser Trp Thr Asp Met Val Ala Lys Gly Glu His Gln Leu Arg
485 490 495
Asn Asp Lys Leu Thr Val Gly Val Gly Thr Thr Glu Gln Thr Glu Ser
500 505 510
Ser Lys His Ala Ser Lys Gln Glu Tyr Arg Thr Pro Pro Pro Ser Gln
515 520 525
Gly Ser Ser Thr Leu His Arg Pro Val Ile Gly Gly His Gln Gln Gly
530 535 540
Phe Ser Ala Asn Ser Trp Arg Arg Ser Asn Ser Lys Ile Ser Thr Asp
545 550 555 560
Asn Lys Val Asn Trp Asp Leu Val Arg Ala Ala Pro Thr Trp Ser Lys
565 570 575
His Lys Val Gln Asp His Ser Gly Arg Val Cys Gln Arg Pro Asn Ala
580 585 590
Ala His Leu Lys Glu Asn Thr Ser Gly Ser Lys Gln Ala Pro Trp Arg
595 600 605
Ser Ser Ala Ser Gln Arg Ala Leu Phe Pro Asp Trp Lys Ser Lys Gly
610 615 620
Glu Gly Tyr Gly His Gly Tyr Val Pro Phe Gly Asp Asn Glu His Ser
625 630 635 640
Gln Gly Ser Ser Arg Thr Glu Ala Thr His Arg Trp His Asn Asn Ala
645 650 655
Ala Gly Thr Val Ser Trp Arg Pro Gln Asn Arg Leu Arg Val Phe Gln
660 665 670
Glu Ile Thr Asn Glu Ile Asn Gln Asn Val Val
675 680
<210> 24
<211> 717
<212> PRT
<213> 二穗短柄草（Brachypodium distachyon)
<400> 24
Met Arg Ser Gly Gly Arg Arg Leu Pro Pro Trp Thr Ser Pro Arg Gly
1 5 10 15
His Ala Ala Ala Glu Val Val Ala Pro Thr Gly Trp Ser Pro Arg Thr
20 25 30
Pro Ala Ala Gly Gly Gly Gly Ser Gly Ser Tyr Val Thr Pro Pro Leu
35 40 45
Thr Ala Gly Cys Cys Cys Ser Ser Ser Ser Tyr Arg Val Thr Pro Pro
50 55 60
Ser Ser Gly Gly Gly Cys Thr Ser Leu Leu Thr Pro Pro Val Ser Gly
65 70 75 80
Gly Gly Cys Ser Arg Pro Pro Arg Ala Pro Pro Ala Val Val Asp Ser
85 90 95
Pro Tyr Val Arg Ala Lys Gln Ala Gln Leu Ile Glu Lys Asp Pro Asn
100 105 110
Lys Ala Val Pro Leu Phe Trp Ala Ala Ile Asn Ser Gly Glu Arg Ile
115 120 125
Glu Ser Ala Leu Lys Asp Met Ala Thr Val Leu Lys Gln Ala Asn Arg
130 135 140
Ala Glu Glu Ala Ile Glu Ala Ile Arg Ser Phe Arg Asp Arg Cys Pro
145 150 155 160
Asn Glu Ala Gln Asp Ser Leu Asp Asn Val Leu Leu Asp Leu Tyr Lys
165 170 175
Lys Cys Gly Arg Thr Lys Glu Gln Ile Glu Met Leu Thr Ile Lys Leu
180 185 190
Arg Met Val Asp Glu Asp Leu Ala Ser Gly Arg Trp Lys Thr Lys Leu
195 200 205
Ser Lys Ser His Gly Arg Val Val Tyr Leu Ser Leu Arg Asp Glu Lys
210 215 220
Ala Arg Leu Leu Gly Asn Leu Ala Trp Ala His Met Gln Ser Glu Asn
225 230 235 240
Tyr Glu Glu Ala Glu Met Leu Tyr Arg Gln Ala Leu Ala Ile Glu Ala
245 250 255
Asp Tyr Asn Lys Glu Cys Asn Leu Ala Val Cys Leu Met Lys Thr Gly
260 265 270
Lys Val Ala Glu Ala Lys Tyr Leu Leu Gln Ala Ile Pro Tyr Asn Ser
275 280 285
Ser Asp Glu Lys His Val Arg Ser Phe Ala Arg Ala Thr Glu Met Ile
290 295 300
Lys Glu Leu Glu Ser Gln Ala Leu Pro Ser Pro Ile Thr Gln Met Lys
305 310 315 320
Ser Lys Asp Ser Arg Ile Leu Leu Ala Thr Asp Ala Glu Asn Leu Asp
325 330 335
Tyr Ile Gln Pro Glu Ile Leu Ser Thr Ser Ser Thr Gln Leu Gln Tyr
340 345 350
Glu Glu Pro Glu Phe Ser Val Ser Ala Asp Thr Glu Lys Gln Val Asp
355 360 365
Cys Asn Ser Gln Glu Leu Pro Ser Pro Ile Thr Gln Leu Lys Arg Lys
370 375 380
Val Pro Gln Ile Met Val Asp Ser Glu Lys Asn Gly Glu Cys Pro Glu
385 390 395 400
Glu Asn Gln Asp Ile Ser Arg Leu Phe Asn Asp Ala Ala Thr Pro Gln
405 410 415
Ser Leu Leu Glu Lys Leu Arg Lys Arg Leu Val Lys Lys Asp Arg Pro
420 425 430
Asn Ile Ser Ile Gln His Gln Ala Gln Thr Pro Ser Ser Thr Glu Cys
435 440 445
Leu Pro Ile Cys Asn Gly Ala Thr Asp Ala Ser Asp Asn His Leu Gln
450 455 460
Glu Gly Gln Ser Ser Val Gly Gly Ala Arg Lys Thr Trp Ala Asp Met
465 470 475 480
Val Glu Glu Asp Glu Gln Gln Leu Gly Asp Gly Lys Ser Gly Thr Ala
485 490 495
Gln Asn Glu Ser Ser Lys Gln Ala Ser Glu Gln Arg Tyr Ile Thr Pro
500 505 510
Pro Ser Ser Gln Ala Asn Ser Thr Leu Gln Thr Pro Ala Ala Gly Val
515 520 525
Arg Leu Gln Ser Ser Ser Ala Gly Ser Trp Arg Arg Lys Asp Ser Thr
530 535 540
Gly Glu Asn Val Asn Arg Lys Phe Val Arg Thr Ala Pro Ala Trp Arg
545 550 555 560
Gln Gln Lys Val Gln Asp His Ser Asn Arg Val Cys Gln Arg Leu Asn
565 570 575
Thr Ile His Leu Gly Glu Lys Ala Gln Gly Gln Gly Ala Glu Gln Thr
580 585 590
Pro Trp Arg Ser Ser Ala Ala Gln Arg Ser Leu Phe His Gly His Leu
595 600 605
Pro Ser Gly Glu Ser Gly Arg Cys His Val Ser Gly His Thr Glu Ala
610 615 620
Val Asn Arg Trp Pro Lys Asn Ala Ala Ala Ile Thr Arg Pro Trp Arg
625 630 635 640
Ala Gln Asn Arg Leu Arg Val Phe Gln Glu Ile Thr Asn Glu Met Asn
645 650 655
Arg Asn Val Ala Tyr Glu His Cys Cys Leu Pro Cys Met Gln Thr Leu
660 665 670
Cys Ala Cys Thr Phe Trp Arg Tyr Cys Leu Asp Val Thr Ala Trp Phe
675 680 685
Ile Cys Cys Asn Val Leu His Val Ala Phe Ile Cys Tyr Cys Cys Cys
690 695 700
Cys Trp Leu Ala Gly Trp Leu Ala Gly Gln Phe Pro Ala
705 710 715
<210> 25
<211> 815
<212> PRT
<213> 粳稻（Oryza sativa japonica）
<400> 25
Met Pro Ser Gly Gly Arg Arg Leu Pro Pro Trp Thr Ser Pro Arg Gly
1 5 10 15
Ala Ala Pro Arg Trp Ser Pro Cys Thr Pro Ala Gly Ala Asp Gly Ser
20 25 30
Gly Arg Ala Ala His Ala Thr Pro Pro Ala Ser Gly Gly Cys Ser Ser
35 40 45
His Val Thr Pro Pro Ala Ser Gly Gly Gly Gly Cys Tyr Gly Tyr Arg
50 55 60
Val Thr Pro Pro Thr Ser Gly Gly Cys Ser Arg Pro Pro Arg Ala Pro
65 70 75 80
Leu Ser Ser Val Asp Ser Pro Tyr Val Arg Ala Lys Gln Ala Gln Val
85 90 95
Ile Glu Lys Asp Pro Asn Lys Ala Val Pro Leu Phe Trp Ala Ala Ile
100 105 110
Asn Ser Gly Asp Arg Ile Glu Ser Ala Leu Lys Asp Met Ala Thr Val
115 120 125
Leu Lys Gln Ala Asn Arg Ala Glu Glu Ala Ile Glu Ala Ile Arg Ser
130 135 140
Phe Arg Asp Arg Cys Pro Asn Glu Ala Gln Glu Ser Leu Asp Asn Ile
145 150 155 160
Leu Leu Asp Leu Tyr Lys Lys Cys Gly Arg Thr Lys Glu Gln Ile Glu
165 170 175
Met Leu Thr Leu Lys Leu Arg Ile Val Asp Glu Glu Leu Ala Ser Gly
180 185 190
Arg Trp Lys Thr Lys Leu Ser Lys Ser His Gly Arg Val Val Tyr Leu
195 200 205
Ser Leu Arg Asp Glu Lys Ala Arg Leu Leu Gly Asn Leu Ala Trp Ala
210 215 220
His Met Gln Ser Glu Asn Tyr Asp Glu Ala Glu Met Leu Tyr Arg Gln
225 230 235 240
Ala Leu Ala Ile Glu Ala Asp Tyr Asn Lys Glu Cys Asn Leu Ala Ile
245 250 255
Cys Leu Ile Lys Thr Gly Lys Val Ala Glu Ala Lys Tyr Leu Leu Gln
260 265 270
Ser Ile Pro Asp Asn Cys Ser Asp Glu Ser His Val Arg Ser Leu Ala
275 280 285
Arg Ala Arg Glu Met Leu Met Glu Leu Glu Ser Pro Thr Leu His Ser
290 295 300
Pro Ile Thr Gln Met Lys Ser Lys Glu Ser Leu Ile Trp Leu Ala Ile
305 310 315 320
Asp Ala Glu Asn Leu Gly His Leu Gln Pro Gln Val Ser Ser Thr Ala
325 330 335
Leu Thr Gln Leu Lys Ser Glu Glu Pro His Ile Ser Val Ala Ala Asp
340 345 350
Ala Glu Lys Gln Glu Asp Cys Asn Ser Gln Val Phe Pro Ser Pro Ile
355 360 365
Thr Gln Met Lys Arg Glu Glu Pro Glu Ser Leu Ile Ala Thr Ser Gly
370 375 380
Glu Asn Asn Glu Lys Cys Leu Asn Glu Tyr Gln Asp Leu Ser Arg Leu
385 390 395 400
Phe Asn Asp Ala Ala Thr Pro Gln Ser Leu Leu Glu Lys Leu Arg Lys
405 410 415
Arg Leu Val Lys Glu Asp Thr Leu Asn Ile Ser Ile Gln His Gln Val
420 425 430
Gln Ile Pro Ser Phe Val Glu Cys Leu Pro Asn Ser Gly Gly Ser Thr
435 440 445
Asp Ala Gly Glu Asn Thr Arg Pro Glu Gly Lys Ala Leu Val Asn Gly
450 455 460
Val Arg Lys Thr Trp Ala Asp Met Val Glu Glu Asp Glu Arg Gln Leu
465 470 475 480
Gly Asp Val Ser Ser Thr Ile Gly Met Asp Thr Thr Lys Arg Asn Val
485 490 495
Ser Cys Lys His Ala Asn Glu Glu Met Tyr Arg Thr Pro Ser Phe Ser
500 505 510
Gln Glu Ser Ser Ala Leu Lys Arg Ser Ser Val Asp Asp His Pro Gln
515 520 525
Ser Ser Ser Ala Asp Ser Trp Arg His Ser Asp Ser Lys Ile Ser Thr
530 535 540
Asp Glu Asn Val Asn Met Lys Phe Val Arg Thr Ala Pro Gln Trp Arg
545 550 555 560
Gln Gln Lys Val Gln Asp Tyr Ser Asn Arg Val Ser Gln Arg Leu Asp
565 570 575
Thr Ser His Leu Ser Asp Arg Ala Glu Gly Thr Glu Gln Pro Pro Trp
580 585 590
Arg Ser Ser Thr Ala Gln Arg Ser Leu Phe Pro Asp Trp Lys Ser Lys
595 600 605
Cys Glu Arg Tyr Gly His Gly Tyr Val Pro Phe Gly Asp Asn Glu His
610 615 620
Phe Gln Gly Ser Ser His Phe Glu Ala Thr Asn Arg Trp Pro Lys Asn
625 630 635 640
Ala Arg Pro Trp Arg Pro Gln Asn Arg Leu Trp Val Phe Gln Glu Ile
645 650 655
Thr Asn Glu Ile Asn Gln Lys Gln Thr Arg Ala Leu Trp Leu Thr Ile
660 665 670
Tyr Thr Thr Leu Leu Phe Gly Thr Thr Ala Leu Asp Asp Thr Ala Trp
675 680 685
Phe Leu Val Glu Cys Phe His Leu Leu Ala Gly His Leu Leu Leu His
690 695 700
Asp Gly Leu Gln Asp Leu Ser Val Ala Pro Pro Ser Gly Gln Phe Ala
705 710 715 720
Glu Met Ala Met Gln Met His Pro Ala Thr Gly Leu Ser Thr His Trp
725 730 735
Ser Gln Val Asp Ser Pro Pro Leu Lys Leu Arg Glu Gly Ala Asn Met
740 745 750
Val Val Leu Leu Gln Asp Gln Leu Leu Pro Met Ala Phe Ser Ile Ala
755 760 765
Asn Val Cys Glu Asp Cys Ile Met Ala Tyr Met Ser Ala Ser Ala Ser
770 775 780
Ser Val Leu Phe Tyr Thr Ser Cys Leu Asp Met Gln Gln Leu Pro Tyr
785 790 795 800
Thr Gly His Val Ile Thr Ile Asn Lys Ser Glu Ala Ala Thr Ile
805 810 815
<210> 26
<211> 813
<212> PRT
<213> 籼稻（Oryza sativa indica）
<400> 26
Met Pro Ser Gly Gly Arg Arg Leu Pro Pro Trp Thr Ser Pro Arg Gly
1 5 10 15
Ala Ala Pro Arg Trp Ser Pro Cys Thr Pro Ala Gly Ala Asp Gly Ser
20 25 30
Gly Arg Ala Ala His Ala Thr Pro Pro Ala Ser Gly Gly Cys Ser Ser
35 40 45
His Val Thr Pro Pro Ala Ser Gly Gly Gly Gly Cys Tyr Gly Tyr Arg
50 55 60
Val Thr Pro Pro Thr Ser Gly Gly Cys Ser Arg Pro Pro Arg Ala Pro
65 70 75 80
Leu Ser Ser Val Asp Ser Pro Tyr Val Arg Ala Lys Gln Ala Gln Val
85 90 95
Ile Glu Lys Asp Pro Asn Lys Ala Val Pro Leu Phe Trp Ala Ala Ile
100 105 110
Asn Ser Gly Asp Arg Ile Glu Ser Ala Leu Lys Asp Met Ala Thr Val
115 120 125
Leu Lys Gln Ala Asn Arg Ala Glu Glu Ala Ile Glu Ala Ile Arg Ser
130 135 140
Phe Arg Asp Arg Cys Pro Asn Glu Ala Gln Glu Ser Leu Asp Asn Ile
145 150 155 160
Leu Leu Asp Leu Tyr Lys Lys Cys Gly Arg Thr Lys Glu Gln Ile Glu
165 170 175
Met Leu Thr Leu Lys Leu Arg Ile Val Asp Glu Glu Leu Ala Ser Gly
180 185 190
Arg Trp Lys Thr Lys Leu Ser Lys Ser His Gly Arg Val Val Tyr Leu
195 200 205
Ser Leu Arg Asp Glu Lys Ala Arg Leu Leu Gly Asn Leu Ala Trp Ala
210 215 220
His Met Gln Ser Glu Asn Tyr Asp Glu Ala Glu Met Leu Tyr Arg Gln
225 230 235 240
Ala Leu Ala Ile Glu Ala Asp Tyr Asn Lys Glu Cys Asn Leu Ala Ile
245 250 255
Cys Leu Ile Lys Thr Gly Lys Val Ala Glu Ala Lys Tyr Leu Leu Gln
260 265 270
Ser Ile Pro Asp Asn Cys Ser Asp Glu Ser His Val Arg Ser Leu Ala
275 280 285
Arg Ala Arg Glu Met Leu Met Glu Leu Glu Ser Pro Thr Leu His Ser
290 295 300
Pro Ile Thr Gln Met Lys Ser Lys Glu Ser Leu Ile Trp Leu Ala Ile
305 310 315 320
Asp Ala Glu Asn Leu Gly His Leu Gln Pro Gln Val Ser Ser Thr Ala
325 330 335
Leu Thr Gln Leu Lys Ser Glu Glu Pro His Ile Ser Val Ala Ala Asp
340 345 350
Ala Glu Lys Gln Glu Asp Cys Asn Ser Gln Val Phe Pro Ser Pro Ile
355 360 365
Thr Gln Met Lys Arg Glu Glu Pro Glu Ser Leu Ile Ala Thr Ser Gly
370 375 380
Glu Lys Asn Glu Lys Cys Leu Asn Glu Tyr Gln Asp Leu Ser Arg Leu
385 390 395 400
Phe Asn Asp Ala Ala Thr Pro Gln Ser Leu Leu Glu Lys Leu Arg Lys
405 410 415
Arg Leu Val Lys Glu Asp Thr Leu Asn Ile Ser Ile Gln His Gln Val
420 425 430
Gln Ile Pro Ser Phe Val Glu Cys Leu Pro Asn Ser Gly Gly Ser Thr
435 440 445
Asp Ala Gly Glu Asn Thr Arg Pro Glu Gly Lys Ala Leu Val Asn Gly
450 455 460
Val Arg Lys Thr Trp Ala Asp Met Val Glu Glu Asp Glu Arg Gln Leu
465 470 475 480
Gly Asp Val Ser Ser Thr Ile Gly Met Asp Thr Thr Lys Arg Asn Val
485 490 495
Ser Cys Lys His Ala Asn Glu Glu Met Tyr Arg Thr Pro Ser Phe Ser
500 505 510
Gln Glu Ser Ser Ala Leu Lys Arg Ser Ser Val Asp Asp His Pro Gln
515 520 525
Ser Ser Ser Ala Asp Ser Trp Arg His Ser Asp Ser Lys Ile Ser Thr
530 535 540
Asp Glu Asn Val Asn Met Lys Phe Val Arg Thr Ala Pro Gln Trp Arg
545 550 555 560
Gln Lys Lys Val Gln Asp Tyr Ser Asn Arg Val Ser Gln Arg Leu Asp
565 570 575
Thr Ser His Leu Ser Asp Arg Ala Glu Gly Thr Glu Gln Pro Pro Trp
580 585 590
Arg Ser Ser Thr Ala Gln Arg Ser Leu Phe Pro Asp Trp Lys Ser Lys
595 600 605
Cys Glu Arg Tyr Gly His Gly Tyr Val Pro Phe Cys Asp Asn Glu His
610 615 620
Phe Gln Gly Ser Ser His Phe Glu Ala Thr Asn Arg Trp Pro Lys Asn
625 630 635 640
Ala Arg Pro Trp Arg Pro Gln Asn Arg Leu Trp Val Phe Gln Glu Ile
645 650 655
Thr Asn Glu Ile Asn Gln Lys Gln Thr Arg Ala Leu Trp Leu Thr Ile
660 665 670
Tyr Thr Thr Leu Leu Phe Gly Thr Thr Ala Leu Asp Asp Thr Ala Trp
675 680 685
Phe Leu Val Glu Cys Phe His Leu Leu Ala Gly His Leu Leu Leu His
690 695 700
Asp Gly Leu Gln Asp Leu Ser Val Ala Pro Pro Ser Gly Gln Phe Ala
705 710 715 720
Glu Met Ala Met Gln Met His Pro Ala Thr Gly Leu Ser Thr His Trp
725 730 735
Ser Gln Val Asp Ser Pro Pro Leu Lys Leu Arg Glu Gly Ala Asn Met
740 745 750
Val Val Leu Leu Gln Asp Gln Met Leu Pro Met Ala Phe Ser Ile Ala
755 760 765
Asn Val Cys Glu Asp Cys Ile Met Ala Tyr Met Ser Ala Ser Ala Ser
770 775 780
Ser Val Leu Phe Tyr Thr Ser Cys Leu Asp Met Gln Gln Leu Pro Tyr
785 790 795 800
Thr Gly His Val Ile Thr Ile Asn Lys Ser Glu Thr Ala
805 810
<210> 27
<211> 685
<212> PRT
<213> 高粱（Sorghum bicolor）
<400> 27
Met Pro Ser Gly Gly Arg Arg Leu Pro Pro Trp Thr Ser Pro Arg Ser
1 5 10 15
Ala Gly Ala Gly Ala Ala Arg Trp Ser Pro Ala Ala Gly Thr Pro Ala
20 25 30
Ala Ala Gly Gly Gln Arg Ser Gly Ser Gly Tyr Gly Thr Pro Pro Leu
35 40 45
Ser Ala Gly Cys Phe Gly Thr Arg Val Thr Pro Pro Thr Ser Gly Gly
50 55 60
Ala Arg Val Thr Pro Pro Ser Thr Gly Gly Cys Ser Ser Arg Pro Pro
65 70 75 80
Arg Pro Pro Pro Ser Leu Asp Ser Pro Tyr Val Arg Ala Lys Gln Ala
85 90 95
Gln Ile Val Glu Lys Asp Pro Asn Lys Ala Val Pro Leu Phe Trp Ala
100 105 110
Ala Ile Asn Ser Gly Asp Arg Thr Glu Ser Ala Leu Lys Asp Met Ala
115 120 125
Asn Val Leu Lys Gln Ala Asn Arg Ala Glu Glu Ala Ile Glu Ala Ile
130 135 140
Arg Ser Phe Arg Asp Arg Cys Pro Tyr Glu Ala Gln Glu Ser Leu Asp
145 150 155 160
Asn Ile Leu Leu Asp Leu Tyr Lys Lys Cys Gly Arg Thr Asp Glu Gln
165 170 175
Ile Glu Met Leu Thr Ile Lys Leu Arg Ile Val Asp Glu Glu Leu Ala
180 185 190
Ser Gly Arg Trp Lys Thr Lys Met Ser Lys Ser His Gly Arg Val Val
195 200 205
Tyr Leu Ser Leu Arg Asp Glu Lys Ala Arg Leu Leu Gly Asn Leu Ala
210 215 220
Trp Ala Tyr Met Gln Ser Glu Asn Tyr Glu Gly Ala Glu Met Leu Tyr
225 230 235 240
Arg Gln Ala Leu Ala Ile Glu Ala Asp Tyr Asn Lys Glu Cys Asn Leu
245 250 255
Ala Ile Cys Leu Met Lys Thr Gly Lys Val Ala Glu Ala Lys Tyr Leu
260 265 270
Ile Gln Ala Ile Pro Tyr Asn Cys Asp Asp Glu Ser His Val Lys Ser
275 280 285
Leu Ser Arg Ala Thr Glu Met Leu Arg Glu Leu Glu Leu Gln Ser Leu
290 295 300
Pro Ser Pro Ile Thr Gln Ala Lys Ser Lys Glu Ser Gln Ile Phe Leu
305 310 315 320
Ala Asp Asp Val Glu Met Leu Val Asp Leu Gln Pro Gln Thr Leu Ser
325 330 335
Thr Pro Leu Ser Glu Leu Lys Tyr Lys Arg Pro His Ile Ser Val Ser
340 345 350
Gln Asn Ala Glu Lys His Glu Asn Cys Asn Ser Trp Leu Pro Ser Pro
355 360 365
Ile Thr Gln Leu Arg Arg Glu Glu Pro His Ile Met Val Thr Ala Gly
370 375 380
Ala Glu Lys Asn Glu Ser Phe Ala Glu Phe Gln Asp Leu Ser Arg Leu
385 390 395 400
Phe Asn Asp Ala Ala Thr Pro His Ser Ile Leu Glu Lys Leu Arg Lys
405 410 415
Arg Leu Val Lys Glu Ala Pro Lys Ile Gly Ile His Asp Asp Gln Ile
420 425 430
Gln Thr Pro Ile Pro Thr Glu Cys Leu Pro Asn Ser Glu Arg Asn Leu
435 440 445
Asp Ala Ser Glu Thr Pro Met Gln Glu Gly Lys Leu Leu Thr Lys Gly
450 455 460
Val Lys Lys Thr Trp Ala Asp Met Val Asp Glu Glu Glu Gln Gln Leu
465 470 475 480
Gly Asp Asp Lys Pro Leu Ala Asp Met Val Ala Lys Asp Glu Gln Gln
485 490 495
Leu Gly Glu Ser Lys Ser Thr Leu Gly Val Gly Thr Thr Glu Gln Lys
500 505 510
Glu Ser Ser Lys His Ala Ser Lys Leu Glu Tyr Arg Thr Pro Leu Ala
515 520 525
Ser Gln Glu Ser Arg Thr His Gln Arg Pro Phe Met Gly Gly Gln Leu
530 535 540
Gln Gly Ser Ser Ala Ala Ser Trp Arg Gln Asn Asp Ser Lys Ile Ser
545 550 555 560
Met Asp Lys Asn Val Asn Arg Asp Leu Val Arg Thr Ala Pro Thr Trp
565 570 575
Ser Lys His Lys Ala Gln Asp His Asn Asn Arg Val Trp Gln Arg Leu
580 585 590
Asp Thr Val His Pro His Glu Arg Ala Ser Asp Thr Asn Gln Val Pro
595 600 605
Arg Arg Ser Asn Thr Ser Gln Arg Ala Leu Phe Pro Asp Trp Lys Ser
610 615 620
Lys Gly Glu Gly His Gly His Gly Cys Val Leu Phe Asp Asp Asn Glu
625 630 635 640
Arg Thr Gln Cys Ser Ser His Val Glu Ala Thr His Arg Trp His Asn
645 650 655
Asn Glu Ala Ser Thr Gly Ser Trp Arg Pro Gln Asn Arg Leu Arg Val
660 665 670
Phe Gln Glu Ile Thr Asn Glu Ile Asn Gln Asn Val Val
675 680 685
<210> 28
<211> 690
<212> PRT
<213> 玉米（Zea mays）
<400> 28
Met Pro Ser Gly Gly Arg Arg Leu Pro Pro Trp Thr Ser Pro Arg Ser
1 5 10 15
Ala Gly Ala Pro Met Trp Ser Thr Ala Gly Thr Pro Gly Gly Pro Arg
20 25 30
Pro Gly Pro Gly Tyr Gly Thr Pro Pro Val Ser Ala Gly Cys Phe Gly
35 40 45
Thr Arg Val Thr Pro Pro Thr Ser Gly Gly Thr Arg Val Thr Pro Pro
50 55 60
Thr Ser Gly Gly Ala Gly Ala Arg Val Thr Pro Pro Ser Thr Gly Gly
65 70 75 80
Cys Ser Ser Arg Pro Pro Arg Pro Pro Pro Ser Met Asp Ser Pro Tyr
85 90 95
Val Arg Ala Lys Gln Ala Gln Ile Val Glu Lys Asp Pro Asn Lys Ala
100 105 110
Val Pro Leu Phe Trp Ala Ala Ile Asn Ser Gly Asp Arg Ile Glu Ser
115 120 125
Ala Leu Lys Asp Met Ala Asn Val Leu Lys Gln Ala Asn Arg Ser Glu
130 135 140
Glu Ala Ile Glu Ala Ile Arg Ser Phe Arg Asp Arg Cys Pro Tyr Glu
145 150 155 160
Ala Gln Glu Ser Leu Asp Asn Ile Leu Leu Asp Leu Tyr Lys Lys Cys
165 170 175
Gly Arg Thr Asp Glu Gln Ile Glu Met Leu Thr Leu Lys Leu Arg Ile
180 185 190
Val Asp Glu Glu Leu Ala Ser Gly Arg Trp Lys Thr Lys Leu Ser Lys
195 200 205
Ser His Gly Arg Val Val Tyr Leu Ser Leu Arg Asp Glu Lys Ala Arg
210 215 220
Leu Leu Gly Asn Leu Ala Trp Ala Tyr Met Gln Ser Glu Asn Tyr Glu
225 230 235 240
Glu Ala Glu Met Leu Tyr Arg Gln Ala Leu Ala Ile Glu Ala Asp Tyr
245 250 255
Asn Lys Glu Cys Asn Leu Ala Ile Cys Leu Met Lys Thr Gly Lys Leu
260 265 270
Ala Glu Ala Lys Tyr Leu Ile His Ala Ile Pro Tyr Asn Cys Asn Asp
275 280 285
Glu Ser His Val Lys Ser Leu Ser Arg Ala Thr Glu Met Leu Arg Glu
290 295 300
Phe Asp Leu Gln Ser Leu Pro Ser Pro Ile Thr Gln Ala Lys Ser Lys
305 310 315 320
Glu Pro Arg Asn Phe Val Ala Asp Asp Val Glu Met Leu Val Asp Leu
325 330 335
Gln Pro Gln Thr Leu Ser Thr Pro Phe Ser Glu Leu Lys Tyr Lys Glu
340 345 350
Ala His Ile Ser Val Ser Gln Asn Ala Glu Lys His Glu Asn Cys Asn
355 360 365
Ser Cys Leu Pro Ser Pro Ile Thr Gln Leu Arg Arg Glu Glu Pro His
370 375 380
Thr Met Val Thr Ala Asp Ala Glu Lys Asn Glu Gly Phe Ala Glu Phe
385 390 395 400
Gln Asp Leu Ser Arg Leu Phe Asn Asp Ala Ala Thr Pro His Ser Val
405 410 415
Leu Glu Lys Leu Arg Lys Arg Leu Val Lys Glu Ala Pro Lys Val Ser
420 425 430
Ile His Asp Gln Ile Gln Thr Pro Pro Thr Glu Ser Leu Pro Asn Ser
435 440 445
Glu Arg Asn Leu Asp Ala Ser Glu Thr Ser Val Gln Glu Gly Lys Leu
450 455 460
Leu Thr Lys Gly Val Arg Lys Thr Trp Ser Asp Met Val Asp Glu Glu
465 470 475 480
Glu Gln Gln Leu Gly Asp Asp Lys Pro Trp Ala Asp Met Val Ala Lys
485 490 495
Asp Asp Gln Gln Leu Gly Asp Gly Lys Ser Thr His Gly Val Gly Ile
500 505 510
Thr Glu Gln Asn Glu Ser Ser Lys His Ala Ser Lys Leu Glu Tyr Arg
515 520 525
Thr Pro Ser Ser Ser Gln Glu Ser Arg Thr His Gln Arg Pro Val Met
530 535 540
Gly Gly Gln Leu Gln Gly Ser Ser Ala Gly Ser Trp Arg Arg Ser Asp
545 550 555 560
Ser Lys Ile Tyr Met Asp Lys Asn Val Asn Trp Asp Leu Val Arg Thr
565 570 575
Ala Pro Thr Trp Ser Arg His Lys Val His Asp His Asn Asn Arg Val
580 585 590
Trp Gln Arg Leu His Thr Ile His Pro Arg Glu Arg Ala Ser Gly Thr
595 600 605
Lys Gln Val Pro Arg Arg Ser Asn Thr Ser Gln Arg Ala Leu Phe Pro
610 615 620
Asp Trp Lys Ser Lys Gly Glu Gly Tyr Gly His Gly Tyr Val Pro Phe
625 630 635 640
Asp Asp Asn Glu His Thr Gln Cys Ser Ser His Ile Glu Ala Ala Thr
645 650 655
His Arg Trp His Asn Asn Glu Ala Ser Thr Gly Ser Trp Arg Pro Gln
660 665 670
Asn Arg Leu Arg Val Phe Gln Glu Ile Thr Asn Glu Ile Asn Gln Asn
675 680 685
Val Val
690
<210> 29
<211> 362
<212> PRT
<213> 拟南芥（Arabidopsis thaliana）
<400> 29
Met Glu Gly Lys Phe Ala Ile Ser Glu Ser Thr Asn Leu Leu Gln Arg
1 5 10 15
Ile Lys Asp Phe Thr Gln Ser Val Val Val Asp Leu Ala Glu Gly Arg
20 25 30
Ser Pro Lys Ile Ser Ile Asn Gln Phe Arg Asn Tyr Cys Met Asn Pro
35 40 45
Glu Ala Asp Cys Leu Cys Ser Ser Asp Lys Pro Lys Gly Gln Glu Ile
50 55 60
Phe Thr Leu Lys Lys Glu Pro Gln Thr Tyr Arg Ile Asp Met Leu Leu
65 70 75 80
Arg Val Leu Leu Ile Val Gln Gln Leu Leu Gln Glu Asn Arg His Ala
85 90 95
Ser Lys Arg Asp Ile Tyr Tyr Met His Pro Ser Ala Phe Lys Ala Gln
100 105 110
Ser Ile Val Asp Arg Ala Ile Gly Asp Ile Cys Ile Leu Phe Gln Cys
115 120 125
Ser Arg Tyr Asn Leu Asn Val Val Ser Val Gly Asn Gly Leu Val Met
130 135 140
Gly Trp Leu Lys Phe Arg Glu Ala Gly Arg Lys Phe Asp Cys Leu Asn
145 150 155 160
Ser Leu Asn Thr Ala Tyr Pro Val Pro Val Leu Val Glu Glu Val Glu
165 170 175
Asp Ile Val Ser Leu Ala Glu Tyr Ile Leu Val Val Glu Lys Glu Thr
180 185 190
Val Phe Gln Arg Leu Ala Asn Asp Met Phe Cys Lys Thr Asn Arg Cys
195 200 205
Ile Val Ile Thr Gly Arg Gly Tyr Pro Asp Val Ser Thr Arg Arg Phe
210 215 220
Leu Arg Leu Leu Met Glu Lys Leu His Leu Pro Val His Cys Leu Val
225 230 235 240
Asp Cys Asp Pro Tyr Gly Phe Glu Ile Leu Ala Thr Tyr Arg Phe Gly
245 250 255
Ser Met Gln Met Ala Tyr Asp Ile Glu Ser Leu Arg Ala Pro Asp Met
260 265 270
Lys Trp Leu Gly Ala Phe Pro Ser Asp Ser Glu Val Tyr Ser Val Pro
275 280 285
Lys Gln Cys Leu Leu Pro Leu Thr Glu Glu Asp Lys Lys Arg Thr Glu
290 295 300
Ala Met Leu Leu Arg Cys Tyr Leu Lys Arg Glu Met Pro Gln Trp Arg
305 310 315 320
Leu Glu Leu Glu Thr Met Leu Lys Arg Gly Val Lys Phe Glu Ile Glu
325 330 335
Ala Leu Ser Val His Ser Leu Ser Phe Leu Ser Glu Val Tyr Ile Pro
340 345 350
Ser Lys Ile Arg Arg Glu Val Ser Ser Pro
355 360
<210> 30
<211> 383
<212> PRT
<213> 拟南芥（Arabidopsis thaliana）
<400> 30
Met Glu Glu Ser Ser Gly Leu Ser Ser Met Lys Phe Phe Ser Asp Gln
1 5 10 15
His Leu Ser Tyr Ala Asp Ile Leu Leu Pro His Glu Ala Arg Ala Arg
20 25 30
Ile Glu Val Ser Val Leu Asn Leu Leu Arg Ile Leu Asn Ser Pro Asp
35 40 45
Pro Ala Ile Ser Asp Leu Ser Leu Ile Asn Arg Lys Arg Ser Asn Ser
50 55 60
Cys Ile Asn Lys Gly Ile Leu Thr Asp Val Ser Tyr Ile Phe Leu Ser
65 70 75 80
Thr Ser Phe Thr Lys Ser Ser Leu Thr Asn Ala Lys Thr Ala Lys Ala
85 90 95
Phe Val Arg Val Trp Lys Val Met Glu Ile Cys Phe Gln Ile Leu Leu
100 105 110
Gln Glu Lys Arg Val Thr Gln Arg Glu Leu Phe Tyr Lys Leu Leu Cys
115 120 125
Asp Ser Pro Asp Tyr Phe Ser Ser Gln Ile Glu Val Asn Arg Ser Val
130 135 140
Gln Asp Val Val Ala Leu Leu Arg Cys Ser Arg Tyr Ser Leu Gly Ile
145 150 155 160
Met Ala Ser Ser Arg Gly Leu Val Ala Gly Arg Leu Phe Leu Gln Glu
165 170 175
Pro Gly Lys Glu Ala Val Asp Cys Ser Ala Cys Gly Ser Ser Gly Phe
180 185 190
Ala Ile Thr Gly Asp Leu Asn Leu Leu Asp Asn Thr Ile Met Arg Thr
195 200 205
Asp Ala Arg Tyr Ile Ile Ile Val Glu Lys His Ala Ile Phe His Arg
210 215 220
Leu Val Glu Asp Arg Val Phe Asn His Ile Pro Cys Val Phe Ile Thr
225 230 235 240
Ala Lys Gly Tyr Pro Asp Ile Ala Thr Arg Phe Phe Leu His Arg Met
245 250 255
Ser Thr Thr Phe Pro Asp Leu Pro Ile Leu Val Leu Val Asp Trp Asn
260 265 270
Pro Ala Gly Leu Ala Ile Leu Cys Thr Phe Lys Phe Gly Ser Ile Gly
275 280 285
Met Gly Leu Glu Ala Tyr Arg Tyr Ala Cys Asn Val Lys Trp Ile Gly
290 295 300
Leu Arg Gly Asp Asp Leu Asn Leu Ile Pro Glu Glu Ser Leu Val Pro
305 310 315 320
Leu Lys Pro Lys Asp Ser Gln Ile Ala Lys Ser Leu Leu Ser Ser Lys
325 330 335
Ile Leu Gln Glu Asn Tyr Ile Glu Glu Leu Ser Leu Met Val Gln Thr
340 345 350
Gly Lys Arg Ala Glu Ile Glu Ala Leu Tyr Cys His Gly Tyr Asn Tyr
355 360 365
Leu Gly Lys Tyr Ile Ala Thr Lys Ile Val Gln Gly Lys Tyr Ile
370 375 380
<210> 31
<211> 1330
<212> PRT
<213> 拟南芥（Arabidopsis thaliana）
<400> 31
Met Phe Phe Gln His Ser Gln Leu Gln Asn Ser Asp His Leu Leu His
1 5 10 15
Glu Ser Met Ala Asp Ser Asn His Gln Ser Leu Ser Pro Pro Cys Ala
20 25 30
Asn Gly His Arg Ser Thr Ile Ser Leu Arg Asp Asp Gln Gly Gly Thr
35 40 45
Phe Cys Leu Ile Cys Phe Ser Asn Leu Val Ser Asp Pro Arg Ile Pro
50 55 60
Thr Val His Val Ser Tyr Ala Leu His Gln Leu Ser Ile Ala Ile Ser
65 70 75 80
Glu Pro Ile Phe Leu Arg Thr Leu Leu Ser Ser His Ile His Phe Leu
85 90 95
Val Ser Pro Leu Val His Ala Leu Ser Ser Ile Asp Asp Ala Pro Ile
100 105 110
Ala Ile Gln Ile Met Asp Met Ile Ser Leu Leu Cys Ser Val Glu Glu
115 120 125
Ser Ser Ile Gly Glu Asp Phe Val Glu Arg Ile Ser Asp Gln Leu Ser
130 135 140
Ser Gly Ala Leu Gly Trp Ser Arg Arg Gln Leu His Met Leu His Cys
145 150 155 160
Phe Gly Val Leu Met Ser Cys Glu Asn Ile Asp Ile Asn Ser His Ile
165 170 175
Arg Asp Lys Glu Ala Leu Val Cys Gln Leu Val Glu Gly Leu Gln Leu
180 185 190
Pro Ser Glu Glu Ile Arg Gly Glu Ile Leu Phe Ala Leu Tyr Lys Phe
195 200 205
Ser Ala Leu Gln Phe Thr Glu Gln Asn Val Asp Gly Ile Glu Val Leu
210 215 220
Ser Leu Leu Cys Pro Lys Leu Leu Cys Leu Ser Leu Glu Ala Leu Ala
225 230 235 240
Lys Thr Gln Arg Asp Asp Val Arg Leu Asn Cys Val Ala Leu Leu Thr
245 250 255
Ile Leu Ala Gln Gln Gly Leu Leu Ala Asn Ser His Ser Asn Ser Ala
260 265 270
Ser Ser Met Ser Leu Asp Glu Val Asp Asp Asp Pro Met Gln Thr Ala
275 280 285
Glu Asn Val Ala Ala Arg Pro Cys Leu Asn Val Leu Phe Ala Glu Ala
290 295 300
Ile Lys Gly Pro Leu Leu Ser Thr Asp Ser Glu Val Gln Ile Lys Thr
305 310 315 320
Leu Asp Leu Ile Phe His Tyr Ile Ser Gln Glu Ser Thr Pro Ser Lys
325 330 335
Gln Ile Gln Val Met Val Glu Glu Asn Val Ala Asp Tyr Ile Phe Glu
340 345 350
Ile Leu Arg Leu Ser Glu Cys Lys Asp Gln Val Val Asn Ser Cys Leu
355 360 365
Arg Val Leu Asp Leu Phe Ser Leu Ala Glu His Ser Phe Arg Lys Arg
370 375 380
Leu Val Ile Gly Phe Pro Ser Val Ile Arg Val Leu His Tyr Val Gly
385 390 395 400
Glu Val Pro Cys His Pro Phe Gln Ile Gln Thr Leu Lys Leu Ile Ser
405 410 415
Ser Cys Ile Ser Asp Phe Pro Gly Ile Ala Ser Ser Ser Gln Val Gln
420 425 430
Glu Ile Ala Leu Val Leu Lys Lys Met Leu Glu Arg Tyr Tyr Ser Gln
435 440 445
Glu Met Gly Leu Phe Pro Asp Ala Phe Ala Ile Ile Cys Ser Val Phe
450 455 460
Val Ser Leu Met Lys Thr Pro Ser Phe Gly Glu Thr Ala Asp Val Leu
465 470 475 480
Thr Ser Leu Gln Glu Ser Leu Arg His Ser Ile Leu Ala Ser Leu Ser
485 490 495
Leu Pro Glu Lys Asp Ser Thr Gln Ile Leu His Ala Val Tyr Leu Leu
500 505 510
Asn Glu Ile Tyr Val Tyr Cys Thr Ala Ser Thr Ser Ile Asn Met Thr
515 520 525
Ser Cys Ile Glu Leu Arg His Cys Val Ile Asp Val Cys Thr Ser His
530 535 540
Leu Leu Pro Trp Phe Leu Ser Asp Val Asn Glu Val Asn Glu Glu Ala
545 550 555 560
Thr Leu Gly Ile Met Glu Thr Phe His Ser Ile Leu Leu Gln Asn Ser
565 570 575
Asp Ile Gln Ala Lys Glu Phe Ala Glu Leu Leu Val Ser Ala Asp Trp
580 585 590
Phe Ser Phe Ser Phe Gly Cys Leu Gly Asn Phe Cys Thr Asp Asn Met
595 600 605
Lys Gln Arg Ile Tyr Leu Met Leu Ser Ser Leu Val Asp Ile Leu Leu
610 615 620
Glu Gln Lys Thr Gly Ser His Ile Arg Asp Ala Leu His Cys Leu Pro
625 630 635 640
Ser Asp Pro Gln Asp Leu Leu Phe Leu Leu Gly Gln Ala Ser Ser Asn
645 650 655
Asn Gln Glu Leu Ala Ser Cys Gln Ser Ala Ala Leu Leu Ile Phe His
660 665 670
Thr Ser Ser Ile Tyr Asn Asp Arg Leu Ala Asp Asp Lys Leu Val Leu
675 680 685
Ala Ser Leu Glu Gln Tyr Ile Ile Leu Asn Lys Thr Ser Leu Ile Cys
690 695 700
Ala Ile Ser Asp Ser Pro Ala Leu Leu Asn Leu Val Asn Leu Tyr Gly
705 710 715 720
Leu Cys Arg Ser Leu Gln Asn Glu Arg Tyr Gln Ile Ser Tyr Ser Leu
725 730 735
Glu Ala Glu Arg Ile Ile Phe His Leu Leu Asn Glu Tyr Glu Trp Asp
740 745 750
Leu Gly Ser Ile Asn Ile His Leu Glu Ser Leu Lys Trp Leu Phe Gln
755 760 765
Gln Glu Ser Ile Ser Lys Ser Leu Ile Tyr Gln Ile Gln Lys Ile Ser
770 775 780
Arg Asn Asn Leu Ile Gly Asn Glu Val His Asn Val Tyr Gly Asp Gly
785 790 795 800
Arg Gln Arg Ser Leu Thr Tyr Trp Phe Ala Lys Leu Ile Ser Glu Gly
805 810 815
Asp Asn Tyr Ala Ala Thr Leu Leu Val Asn Leu Leu Thr Gln Leu Ala
820 825 830
Glu Lys Glu Glu Gln Glu Asn Asp Val Thr Ser Ile Leu Asn Leu Met
835 840 845
Asn Thr Ile Val Ser Ile Phe Pro Thr Ala Ser Asn Asn Leu Ser Met
850 855 860
Asn Gly Ile Gly Ser Val Val His Arg Leu Val Ser Gly Phe Ser Asn
865 870 875 880
Ser Ser Leu Gly Thr Ser Phe Lys Thr Leu Leu Leu Leu Val Phe Asn
885 890 895
Ile Leu Thr Ser Val Gln Pro Ala Val Leu Met Ile Asp Glu Ser Trp
900 905 910
Tyr Ala Val Ser Ile Lys Leu Leu Asn Phe Leu Ser Leu Arg Asp Thr
915 920 925
Ala Ile Lys Gln Asn His Glu Asp Met Val Val Ile Gly Ile Leu Ser
930 935 940
Leu Val Leu Tyr His Ser Ser Asp Gly Ala Leu Val Glu Ala Ser Arg
945 950 955 960
Asn Ile Val Ser Asn Ser Tyr Leu Val Ser Ala Ile Asn Thr Val Val
965 970 975
Asp Val Ala Cys Ser Lys Gly Pro Ala Leu Thr Gln Cys Gln Asp Glu
980 985 990
Thr Asn Ile Gly Glu Ala Leu Ala Phe Thr Leu Leu Leu Tyr Phe Phe
995 1000 1005
Ser Leu Arg Ser Leu Gln Ile Val Leu Ala Gly Ala Val Asp Trp
1010 1015 1020
Gln Ala Phe Phe Gly Thr Ser Thr Ser Leu Glu Thr Leu Pro Val
1025 1030 1035
Val Cys Ile Tyr Cys His Asn Leu Cys Arg Leu Met His Phe Gly
1040 1045 1050
Ala Pro Gln Ile Lys Leu Ile Ala Ser Tyr Cys Leu Leu Glu Leu
1055 1060 1065
Leu Thr Gly Leu Ser Glu Gln Val Asp Ile Lys Lys Glu Gln Leu
1070 1075 1080
Gln Cys Ser Ser Ser Tyr Leu Lys Ser Met Lys Ala Val Leu Gly
1085 1090 1095
Gly Leu Val Phe Cys Asp Asp Ile Arg Val Ala Thr Asn Ser Ala
1100 1105 1110
Leu Cys Leu Ser Met Ile Leu Gly Trp Glu Asp Met Glu Gly Arg
1115 1120 1125
Thr Glu Met Leu Lys Thr Ser Ser Trp Tyr Arg Phe Ile Ala Glu
1130 1135 1140
Glu Met Ser Val Ser Leu Ala Leu Pro Cys Ser Ala Ser Ser Thr
1145 1150 1155
Tyr Val Asn His His Lys Pro Ala Val Tyr Leu Thr Val Ala Met
1160 1165 1170
Leu Arg Leu Lys Asn Lys Pro Val Trp Leu Arg Thr Val Phe Asp
1175 1180 1185
Glu Ser Cys Ile Ser Ser Met Ile Gln Asn Leu Asn Gly Ile Asn
1190 1195 1200
Ile Ser Arg Glu Ile Val Ile Leu Phe Arg Glu Leu Met Gln Ala
1205 1210 1215
Glu Leu Leu Asn Ser Gln Gln Val Thr Lys Leu Asp Arg Ala Phe
1220 1225 1230
Gln Glu Cys Arg Lys Gln Met His Arg Asn Gly Thr Arg Asp Glu
1235 1240 1245
Thr Val Glu Glu Gln Val Gln Arg Lys Ile Pro Ser Ile His Asp
1250 1255 1260
His Ser Glu Phe Cys Asn Tyr Leu Val His Leu Met Val Ser Asn
1265 1270 1275
Ser Phe Gly His Pro Ser Glu Ser Glu Thr Tyr Thr Gln Lys Lys
1280 1285 1290
Lys Gln Ile Leu Asp Glu Met Glu Gln Leu Ser Glu Leu Ile Ser
1295 1300 1305
Thr Arg Glu Gly Arg Val Ser Pro Ile Gln Glu Glu Thr Arg Gln
1310 1315 1320
Met Gln Thr Glu Arg Ile Val
1325 1330
<210> 32
<211> 385
<212> PRT
<213> 拟南芥（Arabidopsis thaliana）
<400> 32
Met Ser Ser Ser Val Ala Glu Ala Asn His Thr Glu Lys Glu Glu Ser
1 5 10 15
Leu Arg Leu Ala Ile Ala Val Ser Leu Leu Arg Ser Lys Phe Gln Asn
20 25 30
His Gln Ser Ser Ser Ser Thr Ser Arg Cys Tyr Val Ser Ser Glu Ser
35 40 45
Asp Ala Leu Arg Trp Lys Gln Lys Ala Lys Glu Arg Lys Lys Glu Ile
50 55 60
Ile Arg Leu Gln Glu Asp Leu Lys Asp Ala Glu Ser Ser Phe His Arg
65 70 75 80
Asp Leu Phe Pro Ala Asn Ala Ser Cys Lys Cys Tyr Phe Phe Asp Asn
85 90 95
Leu Gly Val Phe Ser Gly Arg Arg Ile Gly Glu Ala Ser Glu Ser Arg
100 105 110
Phe Asn Asp Val Leu Arg Arg Arg Phe Leu Arg Leu Ala Cys Val Val
115 120 125
Ile Leu Ser Leu Ala Arg Arg Arg Ser Arg Arg Lys Leu Thr Arg Ser
130 135 140
Ser Gln Arg Leu Gln Pro Ser Glu Pro Asp Tyr Glu Glu Glu Ala Glu
145 150 155 160
His Leu Arg Ile Ser Ile Asp Phe Leu Leu Glu Leu Ser Glu Ala Asp
165 170 175
Ser Asn Asp Ser Asn Phe Ser Asn Trp Ser His Gln Ala Val Asp Phe
180 185 190
Ile Phe Ala Ser Leu Lys Lys Leu Ile Ser Met Gly Arg Asn Leu Glu
195 200 205
Ser Val Glu Glu Ser Ile Ser Phe Met Ile Thr Gln Leu Ile Thr Arg
210 215 220
Met Cys Thr Pro Val Lys Gly Asn Glu Val Lys Gln Leu Glu Thr Ser
225 230 235 240
Val Gly Phe Tyr Val Gln His Leu Ile Arg Lys Leu Gly Ser Glu Pro
245 250 255
Phe Ile Gly Gln Arg Ala Ile Phe Ala Ile Ser Gln Arg Ile Ser Ile
260 265 270
Leu Ala Glu Asn Leu Leu Phe Met Asp Pro Phe Asp Glu Ser Phe Pro
275 280 285
Glu Met Asp Glu Cys Met Phe Ile Leu Ile Gln Leu Ile Glu Phe Leu
290 295 300
Ile Cys Asp Tyr Leu Leu Pro Trp Ala Asn Glu Ala Phe Asp Asn Val
305 310 315 320
Met Phe Glu Glu Trp Ile Ala Ser Val Val His Ala Arg Lys Ala Val
325 330 335
Lys Ala Leu Glu Glu Arg Asn Gly Leu Tyr Leu Leu Tyr Met Asp Arg
340 345 350
Val Thr Gly Glu Leu Ala Lys Arg Val Gly Gln Ile Thr Ser Phe Arg
355 360 365
Glu Val Glu Pro Ala Ile Leu Asp Lys Ile Leu Ala Tyr Gln Glu Ile
370 375 380
Glu
385
210> 33
<211> 449
<212> PRT
<213> 拟南芥（Arabidopsis thaliana）
<400> 33
Met Lys Met Asn Ile Asn Lys Ala Cys Asp Leu Lys Ser Ile Ser Val
1 5 10 15
Phe Pro Pro Asn Leu Arg Arg Ser Ala Glu Pro Gln Ala Ser Gln Gln
20 25 30
Leu Arg Ser Gln Gln Ser Gln Gln Ser Phe Ser Gln Gly Pro Ser Ser
35 40 45
Ser Gln Arg Gly Cys Gly Gly Phe Ser Gln Met Thr Gln Ser Ser Ile
50 55 60
Asp Glu Leu Leu Ile Asn Asp Gln Arg Phe Ser Ser Gln Glu Arg Asp
65 70 75 80
Leu Ser Leu Lys Lys Val Ser Ser Cys Leu Pro Pro Ile Asn His Lys
85 90 95
Arg Glu Asp Ser Gln Leu Val Ala Ser Arg Ser Ser Ser Gly Leu Ser
100 105 110
Arg Arg Trp Ser Ser Ala Ser Ile Gly Glu Ser Lys Ser Gln Ile Ser
115 120 125
Glu Glu Leu Glu Gln Arg Phe Gly Met Met Glu Thr Ser Leu Ser Arg
130 135 140
Phe Gly Met Met Leu Asp Ser Ile Gln Ser Asp Ile Met Gln Ala Asn
145 150 155 160
Arg Gly Thr Lys Glu Val Phe Leu Glu Thr Glu Arg Ile Gln Gln Lys
165 170 175
Leu Thr Leu Gln Asp Thr Ser Leu Gln Gln Leu Arg Lys Glu Gln Ala
180 185 190
Asp Ser Lys Ala Ser Leu Asp Gly Gly Val Lys Phe Ile Leu Glu Glu
195 200 205
Phe Ser Lys Asp Pro Asn Gln Glu Lys Leu Gln Lys Ile Leu Gln Met
210 215 220
Leu Thr Thr Ile Pro Glu Gln Val Glu Thr Ala Leu Gln Lys Ile Gln
225 230 235 240
Arg Glu Ile Cys His Thr Phe Thr Arg Glu Ile Gln Val Leu Ala Ser
245 250 255
Leu Arg Thr Pro Glu Pro Arg Val Arg Val Pro Thr Ala Pro Gln Val
260 265 270
Lys Ala Lys Glu Asn Leu Pro Glu Gln Arg Gly Gln Ala Ala Lys Val
275 280 285
Leu Thr Ser Leu Lys Met Pro Glu Pro Arg Val Gln Val Pro Ala Ala
290 295 300
Pro Gln Ala Lys Glu Asn Phe Pro Glu Gln Arg Gly Pro Val Ala Lys
305 310 315 320
Ser Asn Ser Phe Cys Asn Thr Thr Leu Lys Thr Lys Gln Pro Gln Phe
325 330 335
Pro Arg Asn Pro Asn Asp Ala Ser Ala Arg Ala Val Lys Pro Tyr Leu
340 345 350
Ser Pro Lys Ile Gln Val Gly Cys Trp Lys Thr Val Lys Pro Glu Lys
355 360 365
Ser Asn Phe Lys Lys Arg Ala Thr Arg Lys Pro Val Lys Ser Glu Ser
370 375 380
Thr Arg Thr Gln Phe Glu Gln Cys Ser Val Val Ile Asp Ser Asp Glu
385 390 395 400
Glu Asp Ile Asp Gly Gly Phe Ser Cys Leu Ile Asn Glu Asn Thr Arg
405 410 415
Gly Thr Asn Phe Glu Trp Asp Ala Glu Lys Glu Thr Glu Arg Ile Leu
420 425 430
Arg Thr Ala Arg Arg Thr Lys Arg Lys Phe Gly Asn Pro Ile Ile Ile
435 440 445
Asn
<210> 34
<211> 233
<212> PRT
<213> 拟南芥（Arabidopsis thaliana）
<400> 34
Met Arg His Asn Ile Lys Phe Lys Ser Lys Gly Thr Leu Lys Ile Arg
1 5 10 15
Asn Thr Ala Gln Ile Ser Leu Trp Lys Lys Cys Ser Asp Ser Met Ile
20 25 30
Ala Asp Gln Thr Tyr Leu Phe Ile Asn Arg Val Gln Asp Arg Arg Phe
35 40 45
Asp Glu Glu Ser Leu Arg Ile Leu Glu Leu Ser Leu Val Ala Met Asn
50 55 60
Val Lys Ser Phe Leu Glu Val Arg Ser Arg Leu Arg Asp Phe Met Arg
65 70 75 80
Ser Glu Ser Val Val Ile Phe Gly Glu Leu Thr Gly Glu Ser Met Val
85 90 95
Ala Lys Leu Ser Val Leu Glu Phe Phe Ala Arg Ala Phe Ala Leu Leu
100 105 110
Gly Asp Met Glu Ser Cys Leu Ala Met Arg Tyr Glu Ala Leu Asn Leu
115 120 125
Arg Gln Leu Lys Ser Pro Ser Cys Leu Trp Leu Gly Val Ser His Ser
130 135 140
Glu Trp Thr Lys Phe Ala Val Gln Ser Met Glu Asn Gly Phe Pro Ser
145 150 155 160
Ile Ala Gly Lys Ala Ser Glu Asn Ala Leu Leu Ser Leu Lys Lys Asp
165 170 175
Ser Leu Ile Glu Pro Lys Ser Glu Asp Asn Ser Asp Ile Leu Asp Ala
180 185 190
Ala Glu Lys Val Arg Arg Leu Arg Asp Ser Ala Ala Ser Leu Thr Ser
195 200 205
Ser His Ser Gly Ile Phe Ile Tyr Ile Val Ser Ser Leu Lys Phe Ala
210 215 220
Val Cys Asn Arg Leu Leu Thr Thr Phe
225 230
<210> 35
<211> 617
<212> PRT
<213> 拟南芥（Arabidopsis thaliana）
<400> 35
Met Phe Tyr Ser His Gln Leu Leu Ala Arg Lys Ala Pro Leu Gly Gln
1 5 10 15
Ile Trp Met Ala Ala Thr Leu His Ala Lys Ile Asn Arg Lys Lys Leu
20 25 30
Asp Lys Leu Asp Ile Ile Gln Ile Cys Glu Glu Ile Leu Asn Pro Ser
35 40 45
Val Pro Met Ala Leu Arg Leu Ser Gly Ile Leu Met Gly Gly Val Val
50 55 60
Ile Val Tyr Glu Arg Lys Val Lys Leu Leu Phe Asp Asp Val Asn Arg
65 70 75 80
Phe Leu Val Glu Ile Asn Gly Ala Trp Arg Thr Lys Ser Val Pro Asp
85 90 95
Pro Thr Leu Leu Pro Lys Gly Lys Thr His Ala Arg Lys Glu Ala Val
100 105 110
Thr Leu Pro Glu Asn Glu Glu Ala Asp Phe Gly Asp Phe Glu Gln Thr
115 120 125
Arg Asn Val Pro Lys Phe Gly Asn Tyr Met Asp Phe Gln Gln Thr Phe
130 135 140
Ile Ser Met Arg Leu Asp Glu Ser His Val Asn Asn Asn Pro Glu Pro
145 150 155 160
Glu Asp Leu Gly Gln Gln Phe His Gln Ala Asp Ala Glu Asn Ile Thr
165 170 175
Leu Phe Glu Tyr His Gly Ser Phe Gln Thr Asn Asn Glu Thr Tyr Asp
180 185 190
Arg Phe Glu Arg Phe Asp Ile Glu Gly Asp Asp Glu Thr Gln Met Asn
195 200 205
Ser Asn Pro Arg Glu Gly Ala Glu Ile Pro Thr Thr Leu Ile Pro Ser
210 215 220
Pro Pro Arg His His Asp Ile Pro Glu Gly Val Asn Pro Thr Ser Pro
225 230 235 240
Gln Arg Gln Glu Gln Gln Glu Asn Arg Arg Asp Gly Phe Ala Glu Gln
245 250 255
Met Glu Glu Gln Asn Ile Pro Asp Lys Glu Glu His Asp Arg Pro Gln
260 265 270
Pro Ala Lys Lys Arg Ala Arg Lys Thr Ala Thr Ser Ala Met Asp Tyr
275 280 285
Glu Gln Thr Ile Ile Ala Gly His Val Tyr Gln Ser Trp Leu Gln Asp
290 295 300
Thr Ser Asp Ile Leu Cys Arg Gly Glu Lys Arg Lys Val Arg Gly Thr
305 310 315 320
Ile Arg Pro Asp Met Glu Ser Phe Lys Arg Ala Asn Met Pro Pro Thr
325 330 335
Gln Leu Phe Glu Lys Asp Ser Ser Tyr Pro Pro Gln Leu Tyr Gln Leu
340 345 350
Trp Ser Lys Asn Thr Gln Val Leu Gln Thr Ser Ser Ser Glu Ser Arg
355 360 365
His Pro Asp Leu Arg Ala Glu Gln Ser Pro Gly Phe Val Gln Glu Arg
370 375 380
Met His Asn His His Gln Thr Asp His His Glu Arg Ser Asp Thr Ser
385 390 395 400
Ser Gln Asn Leu Asp Ser Pro Ala Glu Ile Leu Arg Thr Val Arg Thr
405 410 415
Gly Lys Gly Ala Ser Val Glu Ser Met Met Ala Gly Ser Arg Ala Ser
420 425 430
Pro Glu Thr Ile Asn Arg Gln Ala Ala Asp Ile Asn Val Thr Pro Phe
435 440 445
Tyr Ser Gly Asp Asp Val Arg Ser Met Pro Ser Thr Pro Ser Ala Arg
450 455 460
Gly Ala Ala Ser Ile Asn Asn Ile Glu Ile Ser Ser Lys Ser Arg Met
465 470 475 480
Pro Asn Arg Lys Arg Pro Asn Ser Ser Pro Arg Arg Gly Leu Glu Pro
485 490 495
Val Ala Glu Glu Arg Pro Trp Glu His Arg Glu Tyr Glu Phe Glu Phe
500 505 510
Ser Met Leu Pro Glu Lys Arg Phe Thr Ala Asp Lys Glu Ile Leu Phe
515 520 525
Glu Thr Ala Ser Thr Gln Thr Gln Lys Pro Val Cys Asn Gln Ser Asp
530 535 540
Glu Met Ile Thr Asp Ser Ile Lys Ser His Leu Lys Thr His Phe Glu
545 550 555 560
Thr Pro Gly Ala Pro Gln Val Glu Ser Leu Asn Lys Leu Ala Val Gly
565 570 575
Met Asp Arg Asn Ala Ala Ala Lys Leu Phe Phe Gln Ser Cys Val Leu
580 585 590
Ala Thr Arg Gly Val Ile Lys Val Asn Gln Ala Glu Pro Tyr Gly Asp
595 600 605
Ile Leu Ile Ala Arg Gly Pro Asn Met
610 615
<210> 36
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 合成引物
<400> 36
gacatcggca cttgcttaga g 21
<210> 37
<211> 21
<212> DNA
<213> 人工序列
<220>
<223> 合成引物
<400> 37
gcgatatagc tcccactggt t 21
<210> 38
<211> 31
<212> DNA
<213> 人工序列
<220>
<223> 合成引物
<400> 38
gagtttacta tactctgccg ccggcgagaa c 31
<210> 39
<211> 38
<212> DNA
<213> 人工序列
<220>
<223> 合成引物
<400> 39
ctccacctgg agtttactat gccccgccgc cggcgaga 38
<210> 40
<211> 32
<212> DNA
<213> 人工序列
<220>
<223> 合成引物
<400> 40
ccacctggag ttgcgtatac tccgccgcgg cg 32
<210> 41
<211> 35
<212> DNA
<213> 人工序列
<220>
<223> 合成引物
<400> 41
ccacctggag ttgcgagaac aagtgatcat gtggc 35

Claims (16)

1.一种用于获得产生第二次分裂恢复2n配子的植物的方法，
其中，所述方法包括通过随机诱变或定向诱变，或者通过遗传转化来提供基因内包括显性突变的植物，所述基因指TDM基因，用于编码被指定为TDM蛋白的蛋白；
其中，当所述植物为芸苔属Brassica spp时，所述蛋白与SEQ ID NO:1的TDM蛋白的第1至286位氨基酸残基具有至少75％序列一致性；或者，当所述植物不同于芸苔属Brassicaspp时，所述蛋白与所述残基具有至少30％一致性，并且所述蛋白的前60个氨基酸包括被指定为TPP/Q模体的模体X1X2X3，其中，X1为苏氨酸(T)，X2为脯氨酸(P)，以及X3为脯氨酸(P)或谷氨酰胺(Q)；
并且其中，所述显性突变包括所述TDM蛋白的模体X1X2X3的至少一个残基的突变，并且使所述植物具有产生第二次分裂恢复2n配子的能力。
2.根据权利要求1所述的方法，其中，所述模体位于所述蛋白的SEQ ID NO:1的第16～18位的区域中。
3.根据权利要求1或2所述的方法，其中，所述残基为T残基或与所述T残基相邻的P残基。
4.根据权利要求3所述的方法，其中，所述突变选自由所述T残基和/或P残基被不同残基置换，以及所述T残基和/或P残基的缺失所组成的组，所述T残基和/或P残基被不同残基置换以及所述T残基和/或P残基的缺失单独发生或与所述T残基和/或P残基两侧的1个残基或2个残基一起发生。
5.根据权利要求1至4中任一项所述的方法，其中，所述突变消除在所述模体的T残基处的磷酸化。
6.根据权利要求1至5中任一项所述的方法，所述方法包括：通过随机诱变或定向诱变提供在TDM基因的等位基因中具有所述显性突变的植物，所述植物对于该突变是杂合的。
7.根据权利要求1至5中任一项所述的方法，所述植物为转基因植物，并且所述方法包括：
a)用包含DNA构建体的载体转化至少一个植物细胞，所述DNA构建体包括具有所述显性突变的TDM基因；
b)培养所转化的植物细胞，以使在基因组中具有转基因的植物再生，所述转基因含有所述DNA构建体。
8.一种用于获得产生未减数分裂配子的植物的方法，其中，所述方法包括：
a)提供根据权利要求1至7中任一项所述的在TDM基因中包括所述显性突变的植物；
b)在所述植物中，抑制参与植物中起始减数分裂重组的第一蛋白，所述蛋白选自以下蛋白：
被指定为SPO11-1蛋白的蛋白，其中，所述蛋白与SEQ ID NO:29的SPO11-1蛋白具有至少40％的序列一致性，并且逐渐优选地，具有至少45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白与SEQ ID NO:29的SPO11-1蛋白具有至少60％的序列相似性，并且逐渐优选地，具有至少65％、70％、75％、80％、85％、90％、95％或98％的序列相似性；
被指定为SPO11-2蛋白的蛋白，其中，所述蛋白与SEQ ID NO:30的SPO11-2蛋白具有至少40％的序列一致性，并且逐渐优选地，具有至少45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白与SEQ ID NO:29的SPO11-2蛋白具有至少60％的序列相似性，并且逐渐优选地，具有至少65％、70％、75％、80％、85％、90％、95％或98％的序列相似性；
被指定为PRD1蛋白的蛋白，其中，所述蛋白与SEQ ID NO:31的PRD1蛋白具有至少25％的序列一致性，并且逐渐优选地，具有至少30％、35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白与SEQ ID NO:31的PRD1蛋白具有至少35％的序列相似性，并且逐渐优选地，具有至少40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列相似性；
被指定为PRD2蛋白的蛋白，其中，所述蛋白与SEQ ID NO:32的PRD2蛋白具有至少25％的序列一致性，并且逐渐优选地，具有至少30％、35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白与SEQ ID NO:32的PRD2蛋白具有至少35％的序列相似性，并且逐渐优选地，具有至少40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列相似性；
被指定为PAIR1蛋白的蛋白，其中，所述蛋白与SEQ ID NO:33的PAIR1蛋白具有至少30％的序列一致性，并且逐渐优选地，具有至少35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白与SEQ ID NO:33的PAIR1蛋白具有至少40％的序列相似性，并且逐渐优选地，具有至少45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列相似性；
被指定为DFO蛋白的蛋白，其中，所述蛋白与SEQ ID NO:34的DFO蛋白具有至少30％的序列一致性，并且逐渐优选地，具有至少35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白与SEQ ID NO:34的DFO蛋白具有至少40％的序列相似性，并且逐渐优选地，具有至少45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列相似性；以及
c)在所述植物中，抑制被指定为REC8蛋白的第二蛋白，其中，所述蛋白与SEQ ID NO:35的REC8蛋白具有至少40％的序列一致性，并且逐渐优选地，具有至少45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白与SEQID NO:35的REC8蛋白具有至少45％的序列相似性，并且逐渐优选地，具有至少50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列相似性。
9.根据权利要求8所述的方法，其中，通过诱变编码所述蛋白的基因或其启动子，并且选择部分或全部丢失所述蛋白的活性的突变体，来获得对SPO11-1蛋白、SPO11-2蛋白、PRD1蛋白、PRD2蛋白、DFO蛋白、PAIR1蛋白或REC8蛋白中的至少一种的抑制。
10.根据权利要求8所述的方法，其中，通过在所述植物中表达沉默RNA，来获得对SPO11-1蛋白、SPO11-2蛋白、PRD1蛋白、PRD2蛋白、DFO蛋白、PAIR1蛋白或REC8蛋白中的至少一种的抑制，所述沉默RNA靶向编码所述蛋白的基因。
11.一种DNA构建体，所述DNA构建体包括如权利要求1至5中任一项所述的显性突变的TDM基因。
12.一种产生第二次分裂恢复2n配子的植物，所述植物通过权利要求1至7中任一项所述的方法来得到，其中，所述植物包括在TDM蛋白的模体X1X2X3中的所述显性突变，使所述植物具有产生第二次分裂恢复2n配子的能力。
13.一种产生第二次分裂恢复2n配子的植物，所述植物为包括权利要求11的DNA构建体的转基因的转基因植物。
14.一种产生未减数分裂配子的植物，通过权利要求8至10中任一项所述的方法来得到，其中，所述植物包括在TDM蛋白的模体X1X2X3中的所述显性突变，使所述植物具有产生第二次分裂恢复2n配子的能力。
15.一种用于产生第二次分裂恢复2n配子的方法，其中，所述方法包括培养通过权利要求1至7中任一项的方法所得到的植物，其中，所述植物包括在TDM蛋白的模体X1X2X3中的所述显性突变，使所述植物具有产生第二次分裂恢复2n配子的能力，并且回收由所述植物产生的配子。
16.一种用于产生未减数分裂配子的方法，其中，所述方法包括培养通过权利要求8至10中任一项的方法所得到的植物，其中，所述植物包括在TDM蛋白的模体X1X2X3中的所述显性突变，使所述植物具有产生第二次分裂恢复2n配子的能力，并且回收由所述植物产生的配子。
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