CN106645740B - Chemotactic factor (CF) dosage marker about frequency electromagnetic radiation - Google Patents
Chemotactic factor (CF) dosage marker about frequency electromagnetic radiation Download PDFInfo
- Publication number
- CN106645740B CN106645740B CN201610855233.9A CN201610855233A CN106645740B CN 106645740 B CN106645740 B CN 106645740B CN 201610855233 A CN201610855233 A CN 201610855233A CN 106645740 B CN106645740 B CN 106645740B
- Authority
- CN
- China
- Prior art keywords
- frequency electromagnetic
- chemotactic factor
- marker
- electromagnetic radiation
- serum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01R—MEASURING ELECTRIC VARIABLES; MEASURING MAGNETIC VARIABLES
- G01R29/00—Arrangements for measuring or indicating electric quantities not covered by groups G01R19/00 - G01R27/00
- G01R29/08—Measuring electromagnetic field characteristics
- G01R29/0807—Measuring electromagnetic field characteristics characterised by the application
- G01R29/0814—Field measurements related to measuring influence on or from apparatus, components or humans, e.g. in ESD, EMI, EMC, EMP testing, measuring radiation leakage; detecting presence of micro- or radiowave emitters; dosimetry; testing shielding; measurements related to lightning
- G01R29/0857—Dosimetry, i.e. measuring the time integral of radiation intensity; Level warning devices for personal safety use
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
- G01N2333/52—Assays involving cytokines
- G01N2333/521—Chemokines
Abstract
The invention discloses a kind of methods for detecting frequency electromagnetic radiation comprising following steps: 1) experimental animal is placed in radiation environment；2) experimental animal blood is extracted, serum is separated；3) content for detecting power frequency chemotactic factor (CF) marker in serum, analyzes the case where obtaining power frequency electromagnetic environment.Wherein, frequency electromagnetic radiation chemotactic factor (CF) marker is at least one of MCP-1, EOTAXIN-1.The detection method has accuracy high, and sensitivity is good, using easy feature.
Description
Technical field
The present invention relates to the markers of chemotactic factor (CF), in particular to the chemotactic factor (CF) mark of detectable frequency electromagnetic radiation
Object.
Background technique
Power frequency electromagnetic field is a kind of extremely low frequency electromagnetic fields as caused by the power transmission line and various electric appliances of various voltage class,
Its wavelength is up to 6 000km.Power frequency electromagnetic field in resident living environment is mainly generated by ultra-high-tension power transmission line, is good for by it to human body
The influence of health also more and more attracts people's attention.2000 international cancer research institute (IARC) be classified as power frequency electromagnetic field " can
It is a kind of (2B) to doubt human carcinogen ".Currently, being determined not yet both at home and abroad due to the deficiency of epidemiology and Laboratory evidence
The standard of electromagnetic field exposure limits.For protection environment and staff health, domestic and foreign scholars to different grades of electromagnetic field with
The relationship of human health has carried out the research such as clinical haemocyte, biochemistry, electrolyte.The immune system defence screen important as human body
Barrier causes equally to play critical function in body health influence in extremely low frequency electromagnetic fields.Many researchs concentrate on pair both at home and abroad
Lymphocyte, NK cell, the detection of CD3 cell content, immunoglobulin level analysis, T, the measurement of bone-marrow-derived lymphocyte apoptosis etc.,
But result is not consistent.
It is found in the detection for carrying out related immunological index to the resident to live under living in high voltage power transmission and transforming line corridor, cruelly
Dew group IgM content is significantly higher than control group, and magnetic exposure is prompted to have certain humidification to immune function.Electromagnetic field it is strong
The factors such as degree, exposure duration and space can influence the immunologic function of animal, can be enhanced in some strength and in the time
The immune response of body can inhibit the immunocompetence of body when intensity increases.The study found that long-term work is super at 300,000 volts
Staff in hyperbaric environment, body T lymphocyte apoptosis degree are significantly lower than normal healthy controls crowd, and Apoptosis is very few,
Then inflammatory reaction can be too strong, easily leads to allergy and autoimmunity disease and tumour.
In existing result of study, there are no quick detection extremely low frequency electromagnetic fields to refer to the immunology that body has an impact
Mark can not determine that extremely low frequency electromagnetic fields generate the dose limit for promoting or inhibiting to human body.
Summary of the invention
It is an object of the invention to find the time after body is irradiated by extremely low frequency electromagnetic fields and dosage marker, to make up
The deficiencies in the prior art.
The technical solution used in the present invention is:
1) experimental animal is placed in radiation environment；
2) experimental animal blood is extracted, serum is separated；
3) content for detecting power frequency chemotactic factor (CF) marker in serum, analyzes the case where obtaining power frequency electromagnetic environment.
Preferably, experimental animal is mouse.
Preferably, frequency electromagnetic radiation chemotactic factor (CF) marker is at least one of MCP-1, EOTAXIN-1.
Chemotactic factor (CF) marker provided by the invention has accuracy high, and sensitivity is good, using easy feature.
Detailed description of the invention
Fig. 1 MCP-1 concentration curve；Wherein, (A) is control group and three experimental mice serum MCP-1 each
Concentration value on time point；(B) compared with the concentration for being 0.5mT group mice serum MCP-1 and control group in various time points；
Fig. 2 EOTAXIN-1 concentration curve；Wherein, (A) is control group and three experimental mice serum
Concentration value of the EOTAXIN-1 in various time points；It (B) is 0.5mT group mice serum EOTAXIN-1 and control group when each
Between point on concentration compare.
Specific embodiment
1 animal packet of embodiment and magnetic exposure
Selecting 4~6 week old male BALB/c mouses is research object, totally 290.Experimental group is sudden and violent according to extremely low frequency electromagnetic fields
The dosage difference of dew is divided into 3 groups, respectively 0.1mT group, 0.5mT group, 2.5mT group, and every group 10, every group exposes 8h daily, holds
Continuous irradiation 1 day, 5 days, 10 days, 20 days, 30 days, 60 days, 90 days, control group is equipped with 10 before exposure starts, in lasting exposure
Each number of days sets up parallel control separately.
The collection of 2 serum of embodiment
Every mouse is carried out using eyeball rear vein beard blood taking method to take blood.Every mouse about obtains the periphery 800-1200ul
Blood.After sample stands 4-5 hours in 4 DEG C of refrigerators, 4000rpm, is centrifuged 15 minutes by 4 DEG C, uses immediately or subzero 80 degree dispense
It freezes.Every group of daily 5 sample is respectively collected, each 60 μ l of sample is for detecting cytokine concentrations.
3 ProcartaPlex Multiple detection of embodiment tries process
(1) prepare standard items
1) pipe standards product freeze-drying pipe is taken out, 2000x g is centrifuged 10s
2) the 1X Universal Assay Buffer of 250 μ L is added
3) 30s is mixed gently, 2000x g is centrifuged 10s
4) it is spare to be placed in 5-10min on ice
(2) dilution (4 times) of standard items
1) 8 union of PCR provided in kit is taken out for dilution standard product
2) the hybrid standard product of 200 μ L are added into the first pipe as standard items 1
3) the 1X Universal Assay Buffer of 150 μ L is separately added into pipe 2-7
4) from taking 50 μ L hybrid standard product to be added in pipe 2 in pipe 1,10 mixings of piping and druming, avoid the production of bubble as far as possible up and down
It is raw.
5) pipette tips more renewed are transferred in pipe 3 from the dilution standard product for drawing 50 μ L in pipe 2, are blown and beaten 10 times up and down and are mixed
It is even.The pipette tips more renewed are successively transferred in the 7th pipe in order, and 200 μ l 1X Universal Assay are added in the 8th pipe
Buffer。
6) it is placed in spare on ice
(3) prepare microballoon
1) the Wash Buffer to 1X for diluting 10X is spare, such as by 20mL Wash Buffer Concentrate
180mL ddH is added in (10X)2In O.This dilution can be reserved for half a year at 2-8 DEG C
2) vortex microballoon 30s
3) according to the quantity of sample and standard items, blank control, into 96 orifice plates, the microballoon of 50 μ L is added in every hole
4) 96 orifice plates are put into Magnetic Isolation plate, it is ensured that orifice plate is by stuck fast.To the static 2min of plate, make microballoon heavy
Bottom.Then magnetic sheet is quickly inverted, pours out the liquid in orifice plate.96 orifice plates can not be taken from Magnetic Isolation plate during this
Out.
5) the Wash Buffer of 150 μ L 1X is added into every hole, stands 30s, then magnetic sheet is inverted, is poured out in orifice plate
Liquid
6) in the state of inversion, with the residual liquid of paper handkerchief absorption orifice surface
(4) microballoon and sample incubation
1) the Universal Assay Buffer of 25 μ L is separately added into every hole
2) standard items or sample of 25 μ L are separately added into specified hole
3) 25 μ L Universal Assay Buffer are added into blank control
4) orifice plate sealer, 500rpm shake at room temperature be incubated for 60-120min or room temperature concussion be incubated for 30min, then 4 DEG C it is quiet
It sets and is incubated overnight and (after incubation, carries out next step operation again after needing room temperature concussion to be incubated for 30min)
(5) board-washing
1) 96 orifice plates are placed in Magnetic Isolation plate, stand 2min
2) sealer is gently removed, liquid splash is avoided
3) the liquid inversion in orifice plate is removed
4) 150 μ L Wash Buffer are added into every hole, stand 30s, the liquid inversion in orifice plate is removed.It repeats to walk
Rapid 4, it washes altogether 2 times
5) at the end of last time is cleaned, residual liquid is adsorbed with paper handkerchief
(6) dilution detection antibody (50X → 1X)
Prepare detection antibody diluent according to actual sample amount, every hole needs the dilution of 25 μ L, the antibody ice diluted
It is upper spare.
(7) detection antibody is added
1) the detection antibody after 25 μ L dilution is added into every hole
2) orifice plate is sealed using new sealer
3) 96 orifice plates are taken out from Magnetic Isolation plate, shakes 30min as 500rpm room temperature in the plate oscillator of hole
(8) board-washing
1) 96 orifice plates are placed in Magnetic Isolation plate, stand 2min
2) sealer is gently removed, liquid splash is avoided
3) the liquid inversion in orifice plate is removed
4) 150 μ L Wash Buffer are added into every hole, stand 30s, the liquid inversion in orifice plate is removed.It repeats to walk
Rapid 4, it washes altogether 2 times
5) at the end of last time is cleaned, residual liquid is adsorbed with paper handkerchief
(9) SA-PE is added
1) 50 μ L SA-PE are added into every hole
2) orifice plate is sealed using new sealing film
3) 96 orifice plates are taken out from Magnetic Isolation plate, shakes 30min as 500rpm room temperature in the plate oscillator of hole
(10) board-washing
1) 96 orifice plates are placed in Magnetic Isolation plate, stand 2min
2) sealer is gently removed, liquid splash is avoided
3) the liquid inversion in orifice plate is removed
4) 150 μ L Wash Buffer are added into every hole, stand 30s, the liquid inversion in orifice plate is removed.It repeats to walk
Rapid 4, it washes altogether 2 times
5) at the end of last time is cleaned, residual liquid is adsorbed with paper handkerchief
(11) prepare before machine on
1) 120 μ L Reading Buffer are added into every hole
2) orifice plate is sealed using new sealing film
3) 96 orifice plates are taken out from Magnetic Isolation plate, shakes 5min as 500rpm room temperature in the plate oscillator of hole
4) gently removal seals film, is put into Luminex instrument and reads
Luminex technology testing result: this experiment has detected 9 kinds of chemotactic factor (CF)s: MCP-1, MCP-3, MIP-1 α, MIP-
1β,MIP-2,EOTAXIN-1,IP-10,GRO-α,RANTES.Each chemotactic factor (CF) concentration withIt indicates, such as table 1.
The concentration (pg/ml) of 9 kinds of chemotactic factor (CF)s in different time points in 1 mice serum of table
(note: *, P < 0.05；**,P<0.01；***,P<0.001)
Statistical analysis shows that the difference between exposed group and control group occur on the whole in MCP-1 and Eotaxin-1,
The MCP-1 concentration of 0.5mT group is significantly higher than control group (P < 0.001) (such as Fig. 1)；Three experimental group Eotaxin-1 concentration inequality
Different from control group, but only, 0.5mT group Eotaxin-1 concentration on each time point is above control (P < 0.001) (as schemed
2)。
Claims (2)
1. a kind of method for detecting frequency electromagnetic radiation comprising following steps:
Experimental animal is placed in radiation environment；
Experimental animal blood is extracted, serum is separated；
The content of power frequency chemotactic factor (CF) marker in serum is detected, the case where obtaining power frequency electromagnetic environment is analyzed；The experiment is dynamic
Object is mouse；The chemotactic factor (CF) marker is a kind of dosage marker EOTAXIN-1 of extremely low frequency electromagnetic fields；
The frequency electromagnetic radiation is 0.5mT.
2. application of the chemotactic factor (CF) as the dosage marker of mouse extremely low frequency electromagnetic fields, wherein chemotactic factor (CF) EOTAXIN-
1；The extremely low frequency electromagnetic fields are 0.5mT.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610855233.9A CN106645740B (en) | 2016-09-27 | 2016-09-27 | Chemotactic factor (CF) dosage marker about frequency electromagnetic radiation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610855233.9A CN106645740B (en) | 2016-09-27 | 2016-09-27 | Chemotactic factor (CF) dosage marker about frequency electromagnetic radiation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106645740A CN106645740A (en) | 2017-05-10 |
| CN106645740B true CN106645740B (en) | 2019-02-12 |
Family
ID=58853823
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610855233.9A Active CN106645740B (en) | 2016-09-27 | 2016-09-27 | Chemotactic factor (CF) dosage marker about frequency electromagnetic radiation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106645740B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109295200B (en) * | 2018-09-27 | 2022-04-08 | 南方医科大学 | Biological detection marker for high linear energy transfer ray radiation and application thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012154962A2 (en) * | 2011-05-11 | 2012-11-15 | Stemrad Ltd. | Radiation protection device and methods thereof |
| US20160169901A1 (en) * | 2014-12-10 | 2016-06-16 | The Trustees Of Columbia University In The City Of New York | Methods for diagnosis of cutaneous t-cell lymphoma |
-
2016
- 2016-09-27 CN CN201610855233.9A patent/CN106645740B/en active Active
Non-Patent Citations (3)
| Title |
|---|
| Effect of low frequency magnetic fields on melanoma: tumor inhibition and immune modulation.;Yunzhong Nie, et al.;《BMC Cancer》;20131206;第13卷(第1期);摘要，第2页右栏-第4页右栏，第7页左栏，第8页图5 |
| Modulation of MCP-1 and iNOS by 50-Hz sinusoidal electromagnetic field.;Marcella Reale, et al.;《Nitric Oxide》;20060207;第15卷(第1期);摘要，第51页右栏-第55页左栏 |
| 极低频脉冲电磁场对小鼠免疫功能的影响;李金芳;《生命科学仪器》;20080930;第6卷;摘要，第27页左栏-第28页左栏 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106645740A (en) | 2017-05-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Liu et al. | Epidemiology of environmental exposure and malignant mesothelioma | |
| CN101363860A (en) | Syphilis helicoid antibody chemiluminescence immune assay determination kit and method for preparing same | |
| CN112213427B (en) | Method for detecting nano-scale plastic particles in animal biological sample | |
| CN104020297B (en) | Test kit for m tuberculosis infection detection and clinical therapeutic efficacy monitoring and application thereof | |
| CN102183661B (en) | Application of protein and proteome in preparation of liver cirrhosis diagnostic reagent | |
| CN203083997U (en) | ABO and RhD blood group antigen detection card | |
| CN103604933A (en) | Kit for detecting active tuberculosis based on antigen-specific TNF-alpha-ELISA (enzyme linked immunosorbent assay) and application thereof | |
| CN106645740B (en) | Chemotactic factor (CF) dosage marker about frequency electromagnetic radiation | |
| CN101595389A (en) | Be used for standard serum preparation process of mixture of allergenic activity mensuration and uses thereof | |
| CN107271441A (en) | A kind of method of utilization dark field microscope direct visual perception Cryptosporidium | |
| CN103954755B (en) | A kind of diagnostic kit of mycobacterium tuberculosis latent infection | |
| CN102719353B (en) | Device and method for capturing specificity of circulating cancer cells in peripheral blood | |
| CN203324280U (en) | Reagent box for rapidly detecting neuron-specific enolase | |
| CN204359795U (en) | Mite specific IgG4 subclass antibodies detection kit | |
| CN103217524A (en) | Cystic echinococcosis cyst fluid degreasing antigen for detecting alveolar echinococcosis antibody, and preparation method thereof | |
| CN204347030U (en) | The kit of quick detection syphilis helicoid antibody | |
| Heidari Sareban | Evaluation of Rural Local Management Pattern Regarding of Good Governance Indexes, Case Study: Ardabil County. | |
| CN204086275U (en) | House dust mite allergen specific IgE antibody immue quantitative detection reagent box | |
| CN204495845U (en) | Veterinary microorganism plate agglutination test one-time reaction plate | |
| Cui et al. | Establishment of two novel ELISA methods for Dermatophagoides farinae-specific IgE detection with recombinant group 2 allergen | |
| WO2023248624A1 (en) | Method, device, and program for detecting and quantifying protein | |
| CN100580453C (en) | Kit for investigating body immunity level of hepatitis B patient and preparation method thereof | |
| CN102830205A (en) | Identification method of disease grade of potato late blight and identification plate thereof | |
| Garcia-Beltran et al. | Remote fingerstick blood collection for SARS-CoV-2 antibody testing (preprint) | |
| Mohamed et al. | Detection of acute toxoplasmosis in rural women in Sudan using different diagnostic tests |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210428 Address after: 510515 no.1023 Jingxi street, shatai Road, Baiyun District, Guangzhou City, Guangdong Province Patentee after: Guangzhou South Medical University Science Park Co.,Ltd. Address before: 510515, No. 1023 Sha Sha Road, Baiyun District, Guangdong, Guangzhou Patentee before: SOUTHERN MEDICAL University |
|
| TR01 | Transfer of patent right |