CN106644652B - Automatic dehydration transparent system for biological tissue - Google Patents
Automatic dehydration transparent system for biological tissue Download PDFInfo
- Publication number
- CN106644652B CN106644652B CN201710058220.3A CN201710058220A CN106644652B CN 106644652 B CN106644652 B CN 106644652B CN 201710058220 A CN201710058220 A CN 201710058220A CN 106644652 B CN106644652 B CN 106644652B
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- motor
- objective table
- lead screw
- liquid
- lifting rod
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- 230000018044 dehydration Effects 0.000 title claims abstract description 12
- 238000006297 dehydration reaction Methods 0.000 title claims abstract description 12
- 239000007788 liquid Substances 0.000 claims abstract description 58
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 13
- 239000011521 glass Substances 0.000 claims description 6
- 239000000463 material Substances 0.000 abstract description 3
- 239000012530 fluid Substances 0.000 abstract description 2
- 239000002699 waste material Substances 0.000 description 4
- 238000006073 displacement reaction Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 230000008054 signal transmission Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- External Artificial Organs (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
An automatic biological tissue dehydration transparent system comprises an objective table and a containing container, wherein the objective table is connected with a conveying device, and a magnetic stirrer is arranged below the central position of the objective table; the first lifting rod is connected with a first lead screw, the first lead screw is fixed on a rotating shaft of a first motor, and a first fixing frame is arranged on the lower surface of the objective table; a plurality of liquid guide pipes are arranged on the first lifting rod, and each liquid guide pipe is connected with a liquid inlet peristaltic pump; the second lifting rod is connected with a second lead screw, the second lead screw is fixed on a rotating shaft of a second motor, and a second fixing frame is installed on the lower surface of the objective table; there is the fluid-discharge tube on the second lifter, and the fluid-discharge pump is connected to the leakage fluid dram, still includes the PLC controller, and the feed liquor volume of feed liquor peristaltic pump can be controlled to the PLC controller, and the start time and the end time of agitator can be controlled to the PLC controller. The system automatically finishes the steps of dehydration and transparency after the parameters are set, and saves manpower and material resources.
Description
Technical Field
The invention relates to the field of automation of biological laboratories, in particular to an automatic dehydration transparent system for biological tissues.
Background
The fixation, dehydration and transparency of biological tissues are pretreatment steps of paraffin embedding sections, the operation is complicated, most of the time is soaking waiting, and the time consumption is long. At present, a common laboratory mostly adopts the traditional manual liquid changing and waiting, and a great amount of precious time of researchers is consumed.
Disclosure of Invention
In order to solve the technical problems, the invention provides an automatic dehydration and transparency system for biological tissues, which can automatically complete dehydration and transparency steps after parameters are set, and save manpower and material resources.
In order to achieve the purpose, the invention adopts the scheme that:
an automatic biological tissue dehydration transparent system comprises an objective table and a containing container, wherein a conveying device is connected to the objective table and is driven by a third motor, and a magnetic stirrer is arranged at the central position of the objective table and below the objective table; the object stage is provided with a first through hole, a first lifting rod penetrates through the first through hole to be in threaded connection with a first lead screw, the first lead screw is fixed on a rotating shaft of a first motor, the first motor is fixed on a first fixing frame, and the first fixing frame is arranged on the lower surface of the object stage; a plurality of liquid guide pipes are arranged on the first lifting rod, and each liquid guide pipe is connected with a liquid inlet peristaltic pump; a second through hole is formed in the objective table, a second lifting rod penetrates through the second through hole to be in threaded connection with a second lead screw, the second lead screw is fixed on a rotating shaft of a second motor, the second motor is fixed on a second fixing frame, and the second fixing frame is installed on the lower surface of the objective table; be equipped with the fluid-discharge tube on the second lifter, the fluid-discharge pump is connected to the leakage fluid dram, first motor the second motor the third motor feed liquor peristaltic pump magnetic stirrers and the fluid-discharge pump all is controlled by the PLC controller of installing on the objective table, the feed liquor volume of feed liquor peristaltic pump can be controlled to the PLC controller, the start time and the end time of agitator can be controlled to the PLC controller.
The liquid guide pipe is a glass liquid guide pipe.
The number of the liquid guide pipes is 6.
Threads are arranged inside the rod bodies of the first lifting rod and the second lifting rod; and threads are arranged on the outer surfaces of the rod bodies of the first lead screw and the second lead screw.
The invention has the following beneficial effects: through the leading-in to holding the container of catheter with required liquid, adopt the glass catheter, because the glass catheter plays fine drainage effect, prevent that liquid from splashing, leading-in material resources of using manpower sparingly when having been equipped with a plurality of catheters and having realized multiple liquid. The waste liquid is discharged through the conveying of the conveying device and the liquid discharge pump, so that the automation of the whole process is realized; meanwhile, the PLC can control the starting time and the stopping time of the liquid inlet peristaltic pump, so that the timing of standing time and the timing of stirring time are realized, and the accuracy of biological tissue dehydration is ensured.
Drawings
FIG. 1 is a schematic structural view of the present invention.
Wherein, 1, an object stage; 2, a container is placed; 3 a conveying device; 4 a third motor; 5, a magnetic stirrer; 6 a first lifting rod; 7, a first lead screw; 8 a first motor; 9 a first fixing frame; 10 a catheter; 11 a second lifting rod; 12 a second lead screw; 13 a second motor; 14 a second fixing frame; 15 liquid discharge pipes; 16 positive displacement pumps; 17 a PLC controller; 18 connecting pipes; 19 a waste liquid tank; 20 magnetic stirrer.
Detailed Description
In order to better understand the technical solution of the present invention, the following description is made with reference to the accompanying drawings.
As shown in fig. 1, an automatic dehydration transparent system for biological tissue comprises an objective table 1 and a holding container 2, wherein the objective table is connected with a conveying device 3, the conveying device is driven by a third motor 4, and a magnetic stirrer 5 is arranged at the center of the objective table and below the conveying device; a first through hole is formed in the objective table, a first lifting rod 6 penetrates through the first through hole to be in threaded connection with a first lead screw 7, the first lead screw is fixed on a rotating shaft of a first motor 8, the first motor is fixed on a first fixing frame 9, and the first fixing frame is installed on the lower surface of the objective table; a plurality of liquid guide pipes 10 are arranged on the first lifting rod, and each liquid guide pipe is connected with a liquid inlet peristaltic pump; a second through hole is formed in the objective table, a second lifting rod 11 penetrates through the second through hole to be in threaded connection with a second lead screw 12, the second lead screw is fixed on a rotating shaft of a second motor 13, the second motor is fixed on a second fixing frame 14, and the second fixing frame is installed on the lower surface of the objective table; and a liquid discharge pipe 15 is arranged on the second lifting rod, and the liquid discharge port is connected with a liquid discharge pump 16.
The objective table center department is equipped with the recess, and magnetic stirrers settles in the recess, and magnetic stirrers's upper surface is equipped with pressure sensor, holds the device and responds to whether the device moves to this department through pressure sensor to give the PLC controller with the signal transmission, PLC controller control third motor stall.
The first motor the second motor the third motor the feed liquor peristaltic pump magnetic stirrers and the positive displacement pump all is controlled by the PLC controller 17 of installing on the objective table, the feed liquor volume of feed liquor peristaltic pump can be controlled to the PLC controller, the start time and the end time that the agitator can be controlled to the PLC controller.
The liquid guide pipe is a glass liquid guide pipe.
The number of the liquid guide pipes is 6.
Threads are arranged inside the rod bodies of the first lifting rod and the second lifting rod; and threads are arranged on the outer surfaces of the rod bodies of the first lead screw and the second lead screw.
The lower end of the liquid guide pipe 10 is a liquid guide pipe outlet, and the caliber of the liquid guide pipe outlet is smaller than that of the liquid guide pipe inlet. The liquid guide pipe is connected with the liquid inlet peristaltic pump through a connecting pipe, and the liquid discharge pipe is connected with the liquid discharge pump through a connecting pipe 18. The waste liquid is discharged into a waste liquid tank 19 by a liquid discharge pump.
The liquid guide pipe is a glass liquid guide pipe.
The number of the liquid guide pipes is 6.
When the magnetic stirrer is used, the magnetic stirrer 20 is placed in the container, and the PLC controls the rotation time and duration of the magnetic stirrer by controlling the two-pole electrification condition of the magnetic stirrer base.
Although the embodiments of the present invention have been described with reference to the accompanying drawings, it is not intended to limit the scope of the present invention, and it should be understood by those skilled in the art that various modifications and variations can be made without inventive efforts by those skilled in the art based on the technical solution of the present invention.
Claims (3)
1. An automatic biological tissue dehydration transparent system is characterized by comprising an objective table and a containing container, wherein the objective table is connected with a conveying device, the conveying device is driven by a third motor, and a magnetic stirrer is arranged below the central position of the objective table; the object stage is provided with a first through hole, a first lifting rod penetrates through the first through hole to be in threaded connection with a first lead screw, the first lead screw is fixed on a rotating shaft of a first motor, the first motor is fixed on a first fixing frame, and the first fixing frame is arranged on the lower surface of the object stage; the first lifting rod is provided with a plurality of liquid guide pipes, each liquid guide pipe is connected with a liquid inlet peristaltic pump, the lower end of each liquid guide pipe is a liquid guide pipe outlet, and the caliber of the liquid guide pipe outlet is smaller than that of the liquid guide pipe inlet; the liquid guide pipe is a glass liquid guide pipe; a second through hole is formed in the objective table, a second lifting rod penetrates through the second through hole to be in threaded connection with a second lead screw, the second lead screw is fixed on a rotating shaft of a second motor, the second motor is fixed on a second fixing frame, and the second fixing frame is installed on the lower surface of the objective table; a liquid discharge pipe is arranged on the second lifting rod, a liquid discharge port is connected with a liquid discharge pump, the first motor, the second motor, the third motor, the liquid inlet peristaltic pump, the magnetic stirrer and the liquid discharge pump are all controlled by a PLC (programmable logic controller) installed on an objective table, the PLC can control the liquid inlet amount of the liquid inlet peristaltic pump, and the PLC can control the starting time and the ending time of the stirrer; and a piezoelectric sensor is arranged on the upper surface of the magnetic stirrer, and is used for sensing whether the containing container runs to the position or not and transmitting a signal to the PLC, and the PLC controls the third motor to stop rotating.
2. The system as claimed in claim 1, wherein the number of the liquid guiding tubes is 6.
3. The automatic biological tissue dehydrating and transparent system of claim 1, wherein the first lifting rod and the second lifting rod are provided with screw threads inside; and threads are arranged on the outer surfaces of the rod bodies of the first lead screw and the second lead screw.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710058220.3A CN106644652B (en) | 2017-01-23 | 2017-01-23 | Automatic dehydration transparent system for biological tissue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710058220.3A CN106644652B (en) | 2017-01-23 | 2017-01-23 | Automatic dehydration transparent system for biological tissue |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106644652A CN106644652A (en) | 2017-05-10 |
| CN106644652B true CN106644652B (en) | 2020-01-03 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710058220.3A Active CN106644652B (en) | 2017-01-23 | 2017-01-23 | Automatic dehydration transparent system for biological tissue |
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| Country | Link |
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| CN (1) | CN106644652B (en) |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20010043884A1 (en) * | 1999-12-14 | 2001-11-22 | Ervin Essenfeld | Microwave unit and system for tissue processing |
| US6458598B1 (en) * | 2001-08-13 | 2002-10-01 | Dako A/S | Process for preparing and presenting a tissue sample for histological study |
| CN102192522B (en) * | 2011-06-30 | 2012-10-10 | 国电龙源电力技术工程有限责任公司 | Biomass boiler whole-pack feeding device |
| JP6205122B2 (en) * | 2012-09-21 | 2017-09-27 | あおい精機株式会社 | Sample processing equipment |
| KR101453422B1 (en) * | 2013-04-01 | 2014-10-22 | 박종범 | Sample drier for laboratory |
| CN205120592U (en) * | 2015-11-13 | 2016-03-30 | 无锡艾科瑞思产品设计与研究有限公司 | Little biological activity detector of agricultural product |
| CN205538425U (en) * | 2016-03-01 | 2016-08-31 | 青岛锴锦医疗器械有限公司 | Quick pathological tissue hydroextractor |
| CN205627821U (en) * | 2016-04-20 | 2016-10-12 | 广东椰氏实业股份有限公司 | Automatic liquid preparation device |
| CN105835997B (en) * | 2016-04-29 | 2018-03-16 | 江西欣盛泰实业有限公司 | A kind of public bicycles stop pickup device automatically |
| CN205844057U (en) * | 2016-07-07 | 2016-12-28 | 浙江省农药检定管理所 | A kind of liquid sample processing platform sampling device |
| CN106249449B (en) * | 2016-08-29 | 2019-07-23 | 武汉华星光电技术有限公司 | It is anti-to be caught broken substrate alignment apparatus |
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2017
- 2017-01-23 CN CN201710058220.3A patent/CN106644652B/en active Active
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| Publication number | Publication date |
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| CN106644652A (en) | 2017-05-10 |
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