CN217425424U - Liquid path device - Google Patents
Liquid path device Download PDFInfo
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- CN217425424U CN217425424U CN202221218613.9U CN202221218613U CN217425424U CN 217425424 U CN217425424 U CN 217425424U CN 202221218613 U CN202221218613 U CN 202221218613U CN 217425424 U CN217425424 U CN 217425424U
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Abstract
The utility model discloses a liquid path device, which comprises a liquid distribution bottle, wherein the liquid distribution bottle is connected with a hybridization box, a liquid supply device, a pure water device and an MCU main control board; the liquid path device comprises one or more hybridization liquid storage devices, the hybridization liquid is conveyed into the liquid preparation bottle through a pipeline, the MCU main control board pumps the hybridization liquid and pure water into the liquid preparation bottle through the driving device, and the prepared liquid for experiments is conveyed to the hybridization box. The utility model discloses a liquid way device can prepare required reagent automatically, and reagent ability spot preparation usefulness, and the experiment is effectual.
Description
Technical Field
The utility model belongs to the technical field of medical instrument, in particular to liquid path device.
Background
The gene chip technology application field mainly comprises gene expression profile analysis, new gene discovery, gene mutation and polymorphism analysis, genome library mapping, disease diagnosis and prediction, drug screening, gene sequencing and the like; the method has wide application in agriculture, food supervision, environmental protection, judicial identification and other aspects.
Hybridization experiments are needed in gene chip analysis, hybridization liquid, pure water, hydrogen peroxide, POD (streptavidin coupled peroxidase) or TMB (tetramethylbenzidine) and the like need to be mixed and prepared in the hybridization experiment process, the liquid preparation method commonly used at present is a method of manual operation, after liquid is measured by a test tube, the liquid is poured into a liquid mixing bottle and evenly shaken to prepare the liquid, the operation is complicated, a large amount of manpower and time are consumed, errors or errors of human factors are easily caused, and the experiment effect is influenced.
SUMMERY OF THE UTILITY MODEL
The utility model provides a liquid path device can automatic prepare required reagent, and reagent ability spot preparation usefulness, and experiment effect is good.
One embodiment of the utility model provides a liquid path device, which comprises a liquid distribution bottle, wherein the liquid distribution bottle is connected with a hybridization box, a liquid supply device, a pure water device and an MCU main control board; the liquid path device comprises one or more hybridization liquid storage devices, the hybridization liquid is conveyed into the liquid preparation bottle through a pipeline, the MCU main control board pumps the hybridization liquid and pure water into the liquid preparation bottle through the driving device, and the prepared liquid for experiments is conveyed to the hybridization box.
Preferably, the liquid preparation bottle is also connected with a hydrogen peroxide storage device through a pipeline.
Preferably, the liquid dispensing bottle is further connected with the POD storage device through a pipeline.
Preferably, the liquid preparation bottle is also connected with the TMB storage device through a pipeline.
Preferably, the hybridization cassette is connected to the waste liquid recovery unit through a tube.
Preferably, a stirring device is arranged in the liquid preparation bottle.
Preferably, a heater is arranged at the lower part in the liquid dispensing bottle and is connected with the MCU main control board, a temperature sensor is arranged in the liquid dispensing bottle, and the heater is connected with the temperature sensor.
Preferably, a pressure sensor is arranged in the liquid distribution bottle and is connected with the MCU.
Preferably, the MCU main control board is connected with the touch screen.
Preferably, the pipeline is provided with an eccentric wheel pump, a plunger pump and a switching valve.
The utility model discloses a liquid way device has following beneficial effect:
1. by setting the proportion parameters, the required reagent can be automatically prepared and uniformly stirred, and the reagent is prepared and used at the moment.
2. Can simultaneously prepare the reagent by taking a reagent of which the volume is dozens of microliters to hundreds of milliliters as stock solution without the assistance of a moving shaft.
3. Simple and ingenious design and low cost.
4. Can control the temperature of the reagent in the liquid preparation bottle and monitor the amount of the reagent.
5. Manual operation is replaced, and misoperation is reduced.
Drawings
Fig. 1 is a schematic view of a fluid passage device according to the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, the technical solutions of the present invention will be described in detail below. It is to be understood that the disclosed embodiments are merely exemplary of the invention, and are not intended to limit the invention to the precise embodiments disclosed. Based on the embodiments of the present invention, all other embodiments obtained by a person skilled in the art without creative efforts belong to the protection scope of the present invention.
Referring to fig. 1, in an embodiment of the present invention, a liquid path device includes: the device comprises a liquid dispensing bottle, wherein the liquid dispensing bottle can be connected with a hybridization box, a liquid supply device, a pure water device, a hydrogen peroxide storage device, a POD storage device, a TMB storage device and an MCU master control board; the liquid path device can comprise one or more hybridization liquid storage devices, hybridization liquid, pure water, hydrogen peroxide, POD and TMB are conveyed into the liquid preparation bottle through pipelines, and prepared experimental liquid is conveyed to the hybridization box.
A stirring device is arranged in the liquid preparation bottle. The lower part of the liquid distribution bottle can be provided with a heater which is connected with the MCU main control board, a temperature sensor is arranged in the liquid distribution bottle, and the heater is connected with the temperature sensor.
A pressure sensor can be arranged in the liquid distribution bottle and connected with the MCU.
The MCU main control board can be connected with the touch screen. The pipeline is provided with an eccentric wheel pump, a plunger pump and a switching valve.
The utility model discloses a liquid way device can realize following function
1. The automatic liquid preparation function:
the switching valve is switched to the channel 7, the plunger pump starts to suck liquid, the hybridization liquid 1 enters the plunger pump, the switching valve is switched to the channel 4, the plunger pump pushes the hybridization liquid 1 out of the liquid preparation bottle, the actions are repeated until the hybridization liquid 1 reaches the set liquid amount and is added into the liquid preparation bottle, then the switching valve is switched to the channel 6, the plunger pump sucks pure water, the switching valve is switched to the channel 4, and the plunger pump pushes out the pure water to achieve the effect of cleaning the plunger pump. In the same way, the hybridization solution 2, 3, TMB was added to the solution preparation bottle.
Under default conditions, the channel 2 and the channel 3 of the electromagnetic valve are communicated, the micro peristaltic pump is started, 10 microliter to 200ul of hydrogen peroxide (or pod) is filled into the section of the pipeline between the electromagnetic valve and the liquid dispensing bottle, then the electromagnetic valve works, the channel 2 is communicated with the conduction 1, the switching valve is switched to the channel 6, the plunger pumps pure water, the switching valve is switched to the channel 2, the plunger pump pushes out the pure water, at the moment, the pure water flows out from the channel 2 of the switching valve, the pure water flows out from the inlet 2 of the electromagnetic valve 1, and the reagent remained in the section of the pipeline between the electromagnetic valve and the liquid dispensing bottle is flushed into the liquid dispensing bottle.
After the reagents are added into the bottle, the eccentric wheel pump begins to add pure water to dilute the stock solution proportionally.
2. The automatic blending function: after the solution preparation is completed, the stirrer works to stir the reagent uniformly.
3. Automatic liquid feeding and draining: the multi-channel peristaltic pump firstly sucks a part of reagent to fill the pipeline, redundant reagent is discharged from the funnel, then the reagent in the liquid preparation bottle is pumped into the hybridization box, and the diaphragm pump pumps away the reagent in the hybridization box after the reagent reaction is finished. The above actions are repeated to complete the next reagent reaction.
Description of liquid preparation switch:
the switch of the solution preparation 1 is closed, when the switch is closed, the pipeline system transfers the hybridization solution 1, the hybridization solution 2 and the pure water into the solution preparation bottle according to the set proportion, and the solution 1 is uniformly stirred and the temperature of the solution 1 is controlled to a designated value.
And when the switch is closed, the pipeline system transfers the hybridization solution 1, the hybridization solution 2 and the pure water into the solution preparation bottle according to a set proportion, and the solutions are uniformly stirred and the temperature of the solution 1 is controlled to a specified value.
The switch of the solution preparation 3 is closed, when the switch is closed, the pipeline system transfers the hybridization solution 3 and the pure water into the solution preparation bottle according to the set proportion, and the solution 1 is uniformly stirred and the temperature is controlled to the designated value.
And a solution preparation 4 switch, when the switch is closed, the pipeline system transfers the hybridization solution 1, the hybridization solution 2, the pure water and the pod into the solution preparation bottle according to a set proportion, and the solution 1 is uniformly stirred and the temperature of the solution 1 is controlled to a designated value.
The switch of the solution preparation 5 is closed, when the switch is closed, the pipeline system transfers the hybridization solution 3, the pure water, the H2O2 and the TMB into the solution preparation bottle according to the set proportion, and the solution 1 is stirred uniformly and the temperature is controlled to the designated value.
The above description is only for the specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art can easily think of the changes or substitutions within the technical scope of the present invention, and all should be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (10)
1. A liquid path device is characterized by comprising a liquid distribution bottle, wherein the liquid distribution bottle is connected with a hybridization box, a liquid supply device, a pure water device and an MCU (microprogrammed control Unit) main control board; the liquid path device comprises one or more hybridization liquid storage devices, the hybridization liquid is conveyed into the liquid preparation bottle through a pipeline, the MCU main control board pumps the hybridization liquid and pure water into the liquid preparation bottle through a driving device, and the prepared liquid for experiments is conveyed to the hybridization box.
2. The liquid path device as claimed in claim 1, wherein the liquid preparation bottle is further connected with a hydrogen peroxide storage device through a pipeline.
3. The fluid path set of claim 1 or 2, wherein the solution bottle is further connected to a POD storage device by tubing.
4. The fluid path device according to claim 1 or 2, wherein the fluid dispenser bottle is further connected with a TMB storage device through a pipeline.
5. The fluid path device according to claim 1 or 2, wherein the hybridization cassette is connected to a waste fluid recovery device via a tube.
6. The fluid path device according to claim 1 or 2, wherein a stirring device is arranged in the fluid preparation bottle.
7. The liquid path device as claimed in claim 1 or 2, wherein a heater is arranged at the lower part in the liquid distribution bottle, the heater is connected with the MCU main control board, a temperature sensor is arranged in the liquid distribution bottle, and the heater is connected with the temperature sensor.
8. The fluid path device according to claim 1 or 2, wherein a pressure sensor is arranged in the fluid distribution bottle, and the pressure sensor is connected with the MCU.
9. The fluid path device according to claim 1 or 2, wherein the MCU main control board is connected to a touch screen.
10. The fluid path device according to claim 1 or 2, wherein an eccentric wheel pump, a plunger pump and a switching valve are arranged on the pipeline.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221218613.9U CN217425424U (en) | 2022-05-19 | 2022-05-19 | Liquid path device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221218613.9U CN217425424U (en) | 2022-05-19 | 2022-05-19 | Liquid path device |
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| Publication Number | Publication Date |
|---|---|
| CN217425424U true CN217425424U (en) | 2022-09-13 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221218613.9U Active CN217425424U (en) | 2022-05-19 | 2022-05-19 | Liquid path device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115637218A (en) * | 2022-11-15 | 2023-01-24 | 湖北明德健康科技有限公司 | Liquid path system of gene sequencer and reagent transmission method thereof |
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2022
- 2022-05-19 CN CN202221218613.9U patent/CN217425424U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115637218A (en) * | 2022-11-15 | 2023-01-24 | 湖北明德健康科技有限公司 | Liquid path system of gene sequencer and reagent transmission method thereof |
| CN115637218B (en) * | 2022-11-15 | 2023-08-22 | 邯郸市益林堂医药连锁有限公司 | Liquid path system of gene sequencer and reagent transmission method thereof |
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