CN106635762B - Solid matrix installation for fermenting and its application method - Google Patents
Solid matrix installation for fermenting and its application method Download PDFInfo
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- CN106635762B CN106635762B CN201710145807.8A CN201710145807A CN106635762B CN 106635762 B CN106635762 B CN 106635762B CN 201710145807 A CN201710145807 A CN 201710145807A CN 106635762 B CN106635762 B CN 106635762B
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- 239000007787 solid Substances 0.000 title claims abstract description 67
- 239000011159 matrix material Substances 0.000 title claims abstract description 63
- 238000009434 installation Methods 0.000 title claims abstract description 38
- 238000000034 method Methods 0.000 title claims abstract description 16
- 238000000855 fermentation Methods 0.000 claims abstract description 25
- 230000004151 fermentation Effects 0.000 claims abstract description 25
- 238000003756 stirring Methods 0.000 claims abstract description 16
- 238000011081 inoculation Methods 0.000 claims abstract description 11
- 230000001954 sterilising effect Effects 0.000 claims abstract description 10
- 239000000463 material Substances 0.000 claims description 7
- 239000007921 spray Substances 0.000 claims description 7
- 244000005700 microbiome Species 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims 1
- 230000008014 freezing Effects 0.000 claims 1
- 238000007710 freezing Methods 0.000 claims 1
- 241000894006 Bacteria Species 0.000 abstract description 8
- 238000001035 drying Methods 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 5
- 230000007547 defect Effects 0.000 abstract description 2
- 230000004060 metabolic process Effects 0.000 abstract description 2
- 230000008569 process Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003450 growing effect Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000010563 solid-state fermentation Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/16—Solid state fermenters, e.g. for koji production
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Solid matrix installation for fermenting and its application method, it is related to a kind of solid fermentation device and its application method.It solves the defect that existing solid matrix installation for fermenting does not possess functions/drying after fermentation.Solid matrix installation for fermenting includes housing (1), upper lid (2), stirring vane (3), motor (4), sprayer unit (5) and lyophilized air unit.Application method：First, charging sterilizing；2nd, stirring inoculation；3rd, ferment；4th, it is passed through lyophilized be air-dried.Solid matrix installation for fermenting of the present invention need not will connect the solid matrix after bacterium and remove i.e. achievable drying, avoid the pollution of miscellaneous bacteria, and drying process does not influence the activity of microbe inoculation, the rate of metabolism of microbe inoculation, the activity of the holding microbe inoculation of longer time can be reduced on the contrary.
Description
Technical field
The present invention relates to a kind of solid fermentation device and its application method.
Background technology
Solid fermentation refers to, there is no or almost no in the presence of Free water, there is the water-insoluble solid state substrate of certain humidity
In, the process of biological respinse is carried out with one or more microorganisms, therefore solid state fermentation is biological anti-by continuous phase of gas phase
Answer process.
The used installation for fermenting of current solid matrix fermentation all mainly connects bacterium using atomizing, and which is equal with bacterium is connect
Even the characteristics of；But spraying inevitably adds large quantity of moisture during connecing bacterium, while can also be produced in solid matrix fermentation process
Raw substantial amounts of moisture, causes in the solid matrix of fermentation that free water content is higher, easily triggers a little varied bacteria growings, influences product matter
Amount.But existing solid matrix installation for fermenting does not possess the function of being dried after fermentation.
The content of the invention
The present invention is that do not possess the defect of functions/drying after fermentation to solve existing solid matrix installation for fermenting, and is provided
A kind of solid matrix installation for fermenting and its application method.
Solid matrix installation for fermenting includes housing, upper lid, stirring vane, motor, sprayer unit and lyophilized air and imports dress
Put, stirring vane is connected with machine shaft, sprayer unit is arranged on lid；The lower edge of stirring vane is closely pasted with housing bottom
Close.
The application method of above-mentioned solid matrix installation for fermenting：
First, solid matrix fermented material is added in housing, closure and covered, then heat high-temperature sterilization；
2nd, spray inoculation microorganism while stirring solid matrix fermented material；
3rd, control technological condition for fermentation carries out solid matrix fermentation；
4th, the lyophilized air for being passed through sterilizing is dried, that is, completes solid matrix fermentation.
Solid matrix installation for fermenting of the present invention need not will connect the solid matrix after bacterium and remove i.e. achievable drying, it is to avoid miscellaneous
The pollution of bacterium, and drying process does not influence the activity of microbe inoculation, and the rate of metabolism of microbe inoculation can be reduced on the contrary,
The activity of the holding microbe inoculation of longer time.
The solid matrix fermentation of the similarly suitable dry inoculation anaerobe of apparatus of the present invention.
Solid matrix installation for fermenting of the present invention does not use sealing means, therefore low for the requirement of housing, low manufacture cost；
And have the advantages that simple in construction, rate of drying is fast.
The lower edge of stirring vane is brought into close contact with housing bottom in solid matrix installation for fermenting of the present invention, whipping process hair
Without dead angle inside ferment device, the mixing of solid matrix fermented material is homogeneous, it is thorough to dry.
The solid matrix prepared by solid matrix installation for fermenting of the present invention, because water content is low, nutrition in solid matrix
Thing concentration is homogeneous, uniform with fermentation in the absence of gradient, the growth of thalline, the absorption to nutrients and the secretion of metabolite
Uniform advantage.
Brief description of the drawings
Fig. 1 is the solid matrix installation for fermenting structural profile schematic diagram of embodiment one.
Embodiment
Technical solution of the present invention is not limited to act embodiment set forth below, in addition between each embodiment
Any combination.
Embodiment one：Illustrate present embodiment with reference to Fig. 1, present embodiment solid matrix installation for fermenting includes shell
Body 1, upper lid 2, stirring vane 3, motor 4, sprayer unit 5 and lyophilized air unit, stirring vane 3 connect with machine shaft
Connect, sprayer unit 5 is arranged on lid 2；The lower edge of stirring vane 3 is brought into close contact with the bottom of housing 1.
Present embodiment solid matrix installation for fermenting does not use vacuum or high-pressure process in use, so not using
Overflow in sealing means, the gap that lyophilized air can be between housing and upper lid after being contacted with solid matrix materials.
Embodiment two：The difference of present embodiment and embodiment one is：Solid matrix fermentation dress
It is set to vertical.It is other identical with embodiment one.
Embodiment three：The difference of present embodiment and embodiment one or two is：The solid matrix is sent out
Ferment device case bottom is arc surface.It is other identical with embodiment one or two.
Embodiment four：The difference of present embodiment and one of embodiment one to three is：Lyophilized air
Gatherer includes air sterilization filter plant, gas compressor and pipeline.It is other identical with one of embodiment one to three.
Embodiment five：The difference of present embodiment and one of embodiment one to four is：Housing bottom
It is provided with air inlet.It is other identical with one of embodiment one to four.
Embodiment six：The difference of present embodiment and one of embodiment one to five is：It is described lyophilized
Air unit and fog-spray nozzle UNICOM；Or air nozzle is set on upper lid, freeze air unit and air nozzle UNICOM；
Or lyophilized air unit and air inlet UNICOM.It is other identical with one of embodiment one to five.
Embodiment seven：The difference of present embodiment and one of embodiment one to six is：Fog-spray nozzle with
Air nozzle is arranged on lid middle section.It is other identical with one of embodiment one to six.
Embodiment eight：The difference of present embodiment and one of embodiment one to seven is：Solid matrix
Installation for fermenting has double Shell.It is other identical with one of embodiment one to seven.
Present embodiment solid matrix installation for fermenting has double Shell, the effect with insulation, heating or cooling.
Embodiment nine：Present embodiment is the application method of the solid matrix installation for fermenting of embodiment one：
First, solid matrix fermented material is added in housing, closure and covered, then heat high-temperature sterilization；
2nd, spray inoculation microorganism while stirring solid matrix fermented material；
3rd, control technological condition for fermentation carries out solid matrix fermentation；
4th, the lyophilized air for being passed through sterilizing is dried, and just completes solid matrix fermentation, you can packing.
Embodiment ten：The difference of present embodiment and embodiment nine is：In step 3 fermentation process
Required aseptic gas are passed through by lyophilized air unit.Other steps and parameter are identical with embodiment nine.
Needed for present embodiment is imported during step 2 and three from lyophilized air unit into apparatus of the present invention
Aseptic gas, can with holding meanss for malleation, external microbe will not enter device in, do not cause living contaminants.
Embodiment 11：The difference of present embodiment and embodiment nine or ten is：Step 4 is passed through
It is stirred while the lyophilized air of sterilizing.Other steps and parameter are identical with embodiment nine or ten.
Stirring makes solid matrix formation whirlpool, and solid matrix is collapsed by four circumferential centers, rolled, so as to be filled with lyophilized air
Tap is touched, and accelerates rate of drying.
Embodiment 12：The difference of present embodiment and embodiment nine to 11 is：Housing bottom
It is provided with air inlet；Sterile gas needed for fermentation is passed through into solid matrix installation for fermenting from air inlet in step 3 fermentation process
Body.
Claims (10)
1. solid matrix installation for fermenting, solid matrix installation for fermenting includes housing (1), upper lid (2), stirring vane (3), motor
(4) and sprayer unit (5), it is characterised in that also including freezing air unit, stirring vane (3) is connected with machine shaft,
Sprayer unit (5) is arranged on lid (2)；The lower edge of stirring vane (3) is brought into close contact with housing (1) bottom.
2. solid matrix installation for fermenting according to claim 1, it is characterised in that the solid matrix installation for fermenting is vertical.
3. solid matrix installation for fermenting according to claim 1 or 2, it is characterised in that the solid matrix installation for fermenting housing
Bottom is arc surface.
4. solid matrix installation for fermenting according to claim 1 or 2, it is characterised in that lyophilized air unit includes sky
Gas sterilising filtration equipment, gas compressor and pipeline.
5. solid matrix installation for fermenting according to claim 4, it is characterised in that housing bottom is provided with air inlet.
6. solid matrix installation for fermenting according to claim 5, it is characterised in that the lyophilized air unit and spray
Wu Tou UNICOMs；Or air nozzle is set on upper lid, freeze air unit and air nozzle UNICOM；Or lyophilized air is imported
Device and air inlet UNICOM.
7. solid matrix installation for fermenting according to claim 6, it is characterised in that fog-spray nozzle is arranged on lid with air nozzle
Middle section.
8. solid matrix installation for fermenting according to claim 6, it is characterised in that solid matrix installation for fermenting has bilayer
Housing.
9. the application method of solid matrix installation for fermenting described in claim 1, it is characterised in that solid matrix installation for fermenting makes
Use method：
First, solid matrix fermented material is added in housing, closure and covered, then heat high-temperature sterilization；
2nd, spray inoculation microorganism while stirring solid matrix fermented material；
3rd, control technological condition for fermentation carries out solid matrix fermentation；
4th, the lyophilized air for being passed through sterilizing is dried, that is, completes solid matrix fermentation.
10. solid matrix installation for fermenting application method according to claim 9, it is characterised in that in step 3 fermentation process
Required aseptic gas are passed through by lyophilized air unit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710145807.8A CN106635762B (en) | 2017-03-13 | 2017-03-13 | Solid matrix installation for fermenting and its application method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710145807.8A CN106635762B (en) | 2017-03-13 | 2017-03-13 | Solid matrix installation for fermenting and its application method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106635762A CN106635762A (en) | 2017-05-10 |
| CN106635762B true CN106635762B (en) | 2017-11-07 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710145807.8A Active CN106635762B (en) | 2017-03-13 | 2017-03-13 | Solid matrix installation for fermenting and its application method |
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| Country | Link |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2470365Y (en) * | 2001-02-28 | 2002-01-09 | 食品工业发展研究所 | Deep fermatation apparatus |
| CN101695257A (en) * | 2009-10-30 | 2010-04-21 | 上海芝草生物技术有限公司 | Solid fermentation method for cordyceps sinensis |
| CN102443535A (en) * | 2011-05-10 | 2012-05-09 | 中国科学院过程工程研究所 | Solid fermentation bioreactor |
| CN104004623A (en) * | 2014-06-09 | 2014-08-27 | 泰安生力源生物工程有限公司 | Mass and heat transfer improvement method of solid-state fermentation substrate |
-
2017
- 2017-03-13 CN CN201710145807.8A patent/CN106635762B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2470365Y (en) * | 2001-02-28 | 2002-01-09 | 食品工业发展研究所 | Deep fermatation apparatus |
| CN101695257A (en) * | 2009-10-30 | 2010-04-21 | 上海芝草生物技术有限公司 | Solid fermentation method for cordyceps sinensis |
| CN102443535A (en) * | 2011-05-10 | 2012-05-09 | 中国科学院过程工程研究所 | Solid fermentation bioreactor |
| CN104004623A (en) * | 2014-06-09 | 2014-08-27 | 泰安生力源生物工程有限公司 | Mass and heat transfer improvement method of solid-state fermentation substrate |
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| Publication number | Publication date |
|---|---|
| CN106635762A (en) | 2017-05-10 |
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Effective date of registration: 20210423 Address after: 150028 level 1, unit 2, enterprise accelerator, building 2, innovation and entrepreneurship Plaza, science and technology innovation city, Harbin high tech Industrial Development Zone, Heilongjiang Province Patentee after: Heilongjiang hexufeng Ecological Technology Co.,Ltd. Address before: 2302, room 105, 150001 postal street, Nangang District, Heilongjiang, Harbin Patentee before: Wen Tiejun |
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