CN104004675A - Method for production of lactic acid bacteria by utilizing ethanol industrial yellow water - Google Patents
Method for production of lactic acid bacteria by utilizing ethanol industrial yellow water Download PDFInfo
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Abstract
A method for production of lactic acid bacteria by utilizing ethanol industrial yellow water includes collection and treatment of the ethanol industrial yellow water, cultivation of the lactic acid bacteria in the ethanol industrial yellow water, and receiving and preparation of the lactic acid bacteria. The method for production of the lactic acid bacteria by utilizing the ethanol industrial yellow water not only can make full use of the ethanol industrial yellow water, reduce the treatment cost of the yellow water, optimize comprehensive utilization of the yellow water and improve the ecological environment, but also can expand sources of a nutrition substrate in a conventional lactic acid bacteria production, lower the production costs and expand the application scope of lactic acid bacteria.
Description
Technical field
The present invention relates to comprehensive utilization field and the microorganism culturing field of alcohol industry yellow water, especially utilize the yellow water in alcohol industry to carry out microorganism culturing.
Background technology
Alcohol industry is important energy industry of China, but can produce huge by product in alcohol production process, wherein the solids component such as vinasse can continue to produce the feedstuff raw materials such as DDGS and again be utilized together with part yellow water, and the yellow water of the overwhelming majority is not well utilized.Yellow water is the liquid substance that is rich in multiple nutritional components of discharging in alcohol process producing, and contains abundant organic acid (especially lactic acid in yellow water, butyric acid, caproic acid, the carboxylic acid contents such as acetic acid are maximum), alcohol, aldehyde, esters etc. are fragrance matter, also contain amino acid simultaneously, carbohydrate, alcohol, soil ulmin, yeast thalline autolyzate, microbial cells and viable cell etc., because it is nutritious, directly enter the pollution that tends to cause serious body eutrophication in environment, for this reason, the waste water of national regulation alcohol enterprise discharge must be processed through early stage, and can discharge after reaching 4 grades of water quality standards, so, nutritive ingredient in yellow water is wasted, alcohol enterprise often increases the processing of more expense for yellow water, and most of brewery of China is all to use it for mix grain time cellar for storing things fermentation or support cellar for storing things to the processing of yellow water at present, cultivate the aspect such as artificial distiller's yeast, not only can not efficent use of resources, and cannot fundamentally solve the final outlet problem of fermentation yellow water.Therefore how to fully utilize yellow water resource, how can turn waste into wealth, the outlet problem that thoroughly solves yellow water seems particularly important, if yellow water can be turned waste into wealth, fully utilize, the cost that reduces enterprise is dropped into, also meet the needs of improvement of the ecological environment simultaneously.
Milk-acid bacteria is that a class can produce lactic acid under culture condition, and produces the general name of the probiotics of a series of enzymes simultaneously, and milk-acid bacteria has formed industrial production at present, and its finished product is used widely in the industries such as medicines and health protection, food, feed.But existing milk-acid bacteria production method adopts people for adding a large amount of high-quality nutritive substances to produce as raw material more, its production cost is higher, and then affects the application of milk-acid bacteria, and increases the cost of the related products taking milk-acid bacteria as raw material.
In alcohol industry yellow water, be rich in the nutritive substance of multiple soluble state, therefore, if can utilize alcohol industry yellow water to carry out the production of milk-acid bacteria, can solve the problem of complex utilization of alcohol industry yellow water, turn waste into wealth, can reduce again current milk-acid bacteria production cost, further expand the range of application of milk-acid bacteria.
Summary of the invention
The object of this invention is to provide a kind of technological method that utilizes alcohol industry yellow water to carry out milk-acid bacteria cultivation, reduce on the one hand link and the expense of yellow water processing in alcohol industry, the comprehensive utilization ratio that improves alcohol industry yellow water, reduces the industrial cost of milk-acid bacteria on the other hand.
The technical scheme that the present invention solves the problems of the technologies described above employing is: a kind of method of utilizing alcohol industry yellow water to produce milk-acid bacteria, and concrete steps are as follows:
(1) the general absorbent gauze for alcohol industry yellow water (bilayer) of collecting is filtered, collect filtrate as milk-acid bacteria culture medium, for subsequent use;
(2) the alcohol industry yellow water filtrate of step (1) gained is carried out to the mensuration of nutritive ingredient, and carry out nutritional supplementation according to the nutritional need of measurement result and lactobacter growth breeding, for subsequent use, concrete nutritive ingredient supplements kind and quantity is: often go up in the alcohol industry yellow water filtrate of stating and add urea 2.5g, glucose 10g, tween 80 0.5mL;
(3) the alcohol industry yellow water filtrate after nutritional supplementation in step (2) is carried out to sterilizing, and by pH regulator to 6.3 ~ 6.5 of the alcohol industry yellow water filtrate after sterilizing, for subsequent use, wherein, concrete sterilising method is preferably: by pH regulator to 3.2 ~ 3.5 of the alcohol industry yellow water after supplementing the nutrients, and to injecting carbon dioxide gas in the container of splendid attire alcohol industry yellow water, sealing room temperature are placed 24h;
(4) lactic acid bacteria culturers is inoculated on MRS liquid nutrient medium, and be cultured to the logarithmic growth middle and later periods, it is for subsequent use as kind of daughter bacteria liquid when detection viable count is not less than 1 × 108 cfu/mL, wherein the making method of MRS liquid nutrient medium is: get beef protein powder 10g, beef extract 10g, yeast extract 5g, glucose 20g, sodium-acetate 5g, dibasic ammonium citrate 2g, tween 80 0.1mL, magnesium sulfate 0.58g, manganous sulfate 0. 28g, aquae destillata 1000mL mixing, at 121 DEG C of sterilizing 20min-30min, regulate pH6.3 ~ 6.5 with high-pressure sterilizing pot;
(5) according to 5%(v/v) ratio by step (4) gained kind daughter bacteria liquid be inoculated in the alcohol industry yellow water filtrate of step (3) gained, and cultivate 24 ~ 30h under the condition of 37 ~ 37.5 DEG C;
(6) in step (5), in the milk-acid bacteria of cultivation, add 2% cooked rice flour noodles according to weightmeasurement ratio, and leave standstill 5min-10min under room temperature, then adopt large capacity whizzer, centrifugal in 3000r/min, obtain milk-acid bacteria solid mixture, for subsequent use;
(7) get the milk-acid bacteria solid mixture of step (6) gained, adopt vacuum freeze-drying method to be dried, its concrete drying means is: the milk-acid bacteria solid mixture mixing is spread out into the thickness of 0.3 ~ 0.5cm, be placed in vacuum freeze drier, temperature-45 DEG C ~-50 DEG C, vacuum tightness 10 ~ 20Pa, time of drying, 22 ~ 24h, after being dried, carried out vacuum packaging with laminated film, vacuum tightness is 0.085 ~ 0.095Mpa, can obtain lactobacillus completed product.
Further; before step (7) is carried out; according to weight ratio, in the milk-acid bacteria solid mixture obtaining to step (6), add the cycloheptaamylose of 2.5% ~ 3% sodium alginate, 4.5% ~ 5% skim-milk, 5% glycerine and 8% ~ 10% as protective material, and mix.
Milk-acid bacteria used in the present invention, purchased from Bei Nuo bio tech ltd, Shanghai, is preserved in the biological product of USS collecting center, and its deposit number is ATCC 11007, and buying the date is on October 25th, 2013.
The present invention has following beneficial effect:
First, compare with nutraceutical matrix with the raw material that current milk-acid bacteria produces, the present invention adopts alcohol industry yellow water as cultivating raw material and matrix, take full advantage of alcohol industry waste water, improve the comprehensive utilization value of alcohol industry yellow water, improve ecotope, expanded the source of milk-acid bacteria production desired nutritional matrix, reduced the production cost of milk-acid bacteria;
The second, through the cultivation of milk-acid bacteria, the nutritive substance in alcohol industry yellow water is made full use of by milk-acid bacteria, its content effectively reduces, substantially reach the emission standard of alcohol waste water, and saved link and relevant input of business processes alcohol industry yellow water, reduced the production cost of relevant enterprise;
The 3rd, the present invention directly gathers alcohol industry yellow water from the sheet frame of plate-and-frame filter press, this is because the bleeding point outbalance of alcohol industry yellow water, the yellow water nutritive ingredient that the yellow water nutritive ingredient of directly collecting from the sheet frame of plate-and-frame filter press is collected compared with water port is abundant, is more suitable as culture medium;
The 4th, the present invention, measuring under the prerequisite of alcohol industry yellow water nutrient contg, carries out nutritional supplementation to yellow water targetedly according to measurement result, to be more applicable to the Growth and reproduction of milk-acid bacteria, has further improved the output of milk-acid bacteria in alcohol industry yellow water;
The 5th, for the distinctive chemical constitution of alcohol industry yellow water, carry out the change of suitable pre-treatment and part chemical property, make its Growth and reproduction that is more suitable for milk-acid bacteria, improve the output of milk-acid bacteria;
The 6th, for the distinctive chemical property of alcohol industry yellow water, adopt the method for pH regulator and carbonic acid gas sealing to carry out sterilizing, simple to operate, sterilising effect is better, the carrying out that suitable batch production is produced;
The 7th, in milk-acid bacteria solid mixture freezing dry process, add corresponding protective material, can effectively preserve milk-acid bacteria, make its survival rate reach 85.3%.
Therefore, the method of utilizing alcohol industry yellow water to produce milk-acid bacteria provided by the present invention, can make full use of alcohol industry yellow water, reduce the processing cost of yellow water, optimize the comprehensive utilization of yellow water, improve the ecological environment, can expand again the source of nutraceutical matrix in existing milk-acid bacteria production, reduce production costs, expand the range of application of milk-acid bacteria.
Brief description of the drawings
Fig. 1 is the content of various nutritive ingredients in alcohol industry yellow water.
Fig. 2 is the impacts of different culture temperature on viable count in lactobacillus completed product.
Fig. 3 is the impacts of different incubation times on viable count in lactobacillus completed product.
Embodiment
Below in conjunction with specific embodiment, the present invention is further illustrated.
Embodiment 1: a kind of method of utilizing alcohol industry yellow water to produce milk-acid bacteria, what present method adopted is that corn is the yellow water of raw material production alcohol, concrete steps are as follows:
(1) the general absorbent gauze for alcohol industry yellow water (bilayer) of collecting is filtered, collect filtrate as milk-acid bacteria culture medium, for subsequent use;
(2) filtrate of step (1) gained is carried out to the mensuration of nutritive ingredient, its measurement result is shown in accompanying drawing 1, and carry out nutritional supplementation according to the nutritional need of measurement result and lactobacter growth breeding, concrete nutritive ingredient supplements kind and quantity is: often go up and in the alcohol industry yellow water of stating, add urea 2.5g, glucose 10g, tween 80 0.5mL;
(3) the alcohol industry yellow water filtrate after nutritional supplementation in step (2) is carried out to sterilizing, and by pH regulator to 6.3 ~ 6.5 of the alcohol industry yellow water filtrate after sterilizing, for subsequent use, wherein, concrete sterilising method is: by pH regulator to 3.2 ~ 3.5 of the alcohol industry yellow water filtrate after supplementing the nutrients, and to injecting carbon dioxide gas in the container of splendid attire alcohol industry yellow water filtrate, sealing room temperature are placed 24h;
(4) lactic acid bacteria culturers is inoculated on MRS liquid nutrient medium, and be cultured to the logarithmic growth middle and later periods, it is for subsequent use as kind of daughter bacteria liquid when detection viable count is not less than 1 × 108 cfu/mL, wherein the making method of MRS liquid nutrient medium is: get beef protein powder 10g, beef extract 10g, yeast extract 5g, glucose 20g, sodium-acetate 5g, dibasic ammonium citrate 2g, tween 80 0.1mL, magnesium sulfate 0.58g, manganous sulfate 0. 28g, aquae destillata 1000mL mixing, at 121 DEG C of sterilizing 20min-30min, regulate pH6.3 ~ 6.5 with high-pressure sterilizing pot;
(5) according to 5%(v/v) ratio by step (4) gained kind daughter bacteria liquid be inoculated in the alcohol industry yellow water filtrate of step (3) gained, and cultivate 24h under the condition of 37 DEG C;
(6) in step (5), in the milk-acid bacteria of cultivation, add 2% cooked rice flour noodles according to weightmeasurement ratio, and leave standstill 5min under room temperature, then adopt large capacity whizzer, centrifugal in 3000r/min, obtain milk-acid bacteria solid mixture, for subsequent use;
(7) according to the weight of the milk-acid bacteria solid mixture of step (6) gained, in milk-acid bacteria solid mixture, add 2.5% ~ 3% sodium alginate according to weight ratio, 4.5% ~ 5% skim-milk, 5% glycerine and 8% ~ 10% cycloheptaamylose are as protective material, and mix, then spread out into the thickness of 0.3 ~ 0.5cm, be placed in vacuum freeze drier, in temperature-45 DEG C ~-50 DEG C, under the condition of vacuum tightness 10 ~ 20Pa, carry out lyophilize, time of drying 22 ~ 24h, after being dried, carry out vacuum packaging with laminated film, vacuum tightness is 0.085 ~ 0.095Mpa, can obtain lactobacillus completed product.
After measured, adopting number of viable in the method gained lactobacillus completed product in the present embodiment is (95.7 ± 6) × 10
7.
Embodiment 2: a kind of method of utilizing alcohol industry yellow water to produce milk-acid bacteria, what present method adopted is that corn is the yellow water of raw material production alcohol, concrete steps are as follows:
(1) the general absorbent gauze for alcohol industry yellow water (bilayer) of collecting is filtered, collect filtrate as milk-acid bacteria culture medium, for subsequent use;
(2) filtrate of step (1) gained is carried out to the mensuration of nutritive ingredient, its measurement result is shown in accompanying drawing 1, and carry out nutritional supplementation according to the nutritional need of measurement result and lactobacter growth breeding, concrete nutritive ingredient supplements kind and quantity is: often go up in the alcohol industry yellow water filtrate of stating and add urea 2.5g, glucose 10g, tween 80 0.5mL;
(3) the alcohol industry yellow water filtrate after nutritional supplementation in step (2) is carried out to sterilizing, and by pH regulator to 6.3 ~ 6.5 of the alcohol industry yellow water filtrate after sterilizing, for subsequent use, wherein, concrete sterilising method is: by pH regulator to 3.2 ~ 3.5 of the alcohol industry yellow water filtrate after supplementing the nutrients, and to injecting carbon dioxide gas in the container of splendid attire alcohol industry yellow water filtrate, sealing room temperature are placed 24h;
(4) lactic acid bacteria culturers is inoculated on MRS liquid nutrient medium, and be cultured to the logarithmic growth middle and later periods, it is for subsequent use as kind of daughter bacteria liquid when detection viable count is not less than 1 × 108 cfu/mL, wherein the making method of MRS liquid nutrient medium is: get beef protein powder 10g, beef extract 10g, yeast extract 5g, glucose 20g, sodium-acetate 5g, dibasic ammonium citrate 2g, tween 80 0.1mL, magnesium sulfate 0.58g, manganous sulfate 0. 28g, aquae destillata 1000mL mixing, at 121 DEG C of sterilizing 20min-30min, regulate pH6.3 ~ 6.5 with high-pressure sterilizing pot;
(5) according to 5%(v/v) ratio by step (4) gained kind daughter bacteria liquid be inoculated in the alcohol industry yellow water filtrate of step (3) gained, and cultivate 30h under the condition of 37.5 DEG C;
(6) in step (5), in the milk-acid bacteria of cultivation, add 2% cooked rice flour noodles according to weightmeasurement ratio, and leave standstill 10min under room temperature, then adopt large capacity whizzer, centrifugal in 3000r/min, obtain milk-acid bacteria solid mixture, for subsequent use;
(7) according to the weight of the milk-acid bacteria solid mixture of step (6) gained, in milk-acid bacteria solid mixture, add 2.5% ~ 3% sodium alginate according to weight ratio, 4.5% ~ 5% skim-milk, 5% glycerine and 8% ~ 10% cycloheptaamylose are as protective material, and mix, then spread out into the thickness of 0.3 ~ 0.5cm, be placed in vacuum freeze drier, in temperature-45 DEG C ~-50 DEG C, under the condition of vacuum tightness 10 ~ 20Pa, carry out lyophilize, time of drying 22 ~ 24h, after being dried, carry out vacuum packaging with laminated film, vacuum tightness is 0.085 ~ 0.095Mpa, can obtain lactobacillus completed product.
After measured, adopting number of viable in the method gained lactobacillus completed product in the present embodiment is (98.5 ± 9) × 10
7.
Embodiment 3: a kind of method of utilizing alcohol industry yellow water to produce milk-acid bacteria, what present method adopted is that corn is the yellow water of raw material production alcohol, concrete steps are as follows:
(1) the general absorbent gauze for alcohol industry yellow water (bilayer) of collecting is filtered, collect filtrate as milk-acid bacteria culture medium, for subsequent use;
(2) filtrate of step (1) gained is carried out to the mensuration of nutritive ingredient, its measurement result is shown in accompanying drawing 1, and carry out nutritional supplementation according to the nutritional need of measurement result and lactobacter growth breeding, concrete nutritive ingredient supplements kind and quantity is: often go up in the alcohol industry yellow water filtrate of stating and add urea 2.5g, glucose 10g, tween 80 0.5mL;
(3) the alcohol industry yellow water filtrate after nutritional supplementation in step (2) is carried out to sterilizing, and by pH regulator to 6.3 ~ 6.5 of the alcohol industry yellow water filtrate after sterilizing, for subsequent use, wherein, concrete sterilising method is: by pH regulator to 3.2 ~ 3.5 of the alcohol industry yellow water filtrate after supplementing the nutrients, and to injecting carbon dioxide gas in the container of splendid attire alcohol industry yellow water filtrate, sealing room temperature are placed 24h;
(4) lactic acid bacteria culturers is inoculated on MRS liquid nutrient medium, and be cultured to the logarithmic growth middle and later periods, it is for subsequent use as kind of daughter bacteria liquid when detection viable count is not less than 1 × 108 cfu/mL, wherein the making method of MRS liquid nutrient medium is: get beef protein powder 10g, beef extract 10g, yeast extract 5g, glucose 20g, sodium-acetate 5g, dibasic ammonium citrate 2g, tween 80 0.1mL, magnesium sulfate 0.58g, manganous sulfate 0. 28g, aquae destillata 1000mL mixing, at 121 DEG C of sterilizing 20min-30min, regulate pH6.3 ~ 6.5 with high-pressure sterilizing pot;
(5) according to 5%(v/v) ratio by step (4) gained kind daughter bacteria liquid be inoculated in the alcohol industry yellow water filtrate of step (3) gained, and cultivate 27h under the condition of 37.2 DEG C;
(6) in step (5), in the milk-acid bacteria of cultivation, add 2% cooked rice flour noodles according to weightmeasurement ratio, and leave standstill 7min under room temperature, then adopt large capacity whizzer, centrifugal in 3000r/min, obtain milk-acid bacteria solid mixture, for subsequent use;
(7) according to the weight of the milk-acid bacteria solid mixture of step (6) gained, in milk-acid bacteria solid mixture, add 2.5% ~ 3% sodium alginate according to weight ratio, 4.5% ~ 5% skim-milk, 5% glycerine and 8% ~ 10% cycloheptaamylose are as protective material, and mix, then spread out into the thickness of 0.3 ~ 0.5cm, be placed in vacuum freeze drier, in temperature-45 DEG C ~-50 DEG C, under the condition of vacuum tightness 10 ~ 20Pa, carry out lyophilize, time of drying 22 ~ 24h, after being dried, carry out vacuum packaging with laminated film, vacuum tightness is 0.085 ~ 0.095Mpa, can obtain lactobacillus completed product.
After measured, adopting number of viable in the method gained lactobacillus completed product in the present embodiment is (104.3 ± 7) × 10
7.
Below in conjunction with test example, the present invention is further illustrated.
Test example 1: inquire into and adopt method provided by the present invention to carry out milk-acid bacteria cultivation, the impact of different culture temperature on the quantity of viable bacteria in final lactobacillus completed product, and find out optimum culture temperature.
In the cultivation of microorganism, the impact of temperature is most important, directly has influence on the success or not of microorganism culturing, and whether final colony counts meets the requirements.According to method provided by the invention, adopt single factor design, culture temperature is set to respectively to the differing temps gradient of 36 DEG C, 36.5 DEG C, 36.8 DEG C, 37 DEG C, 37.2 DEG C, 37.5 DEG C, 38 DEG C and 40 DEG C, carries out the cultivation of milk-acid bacteria, test-results is shown in accompanying drawing 2.
By finding out in accompanying drawing 2, while carrying out the cultivation of milk-acid bacteria according to method of the present invention, when culture temperature is during at 37 DEG C to 37.5 DEG C, in milk-acid bacteria, viable count is higher, especially 37.2 DEG C time, viable count is the highest, and therefore, 37.2 DEG C is to adopt method of the present invention to cultivate the optimum culture temperature of milk-acid bacteria.
Test example 2: inquire into and adopt method provided by the present invention to carry out milk-acid bacteria cultivation, the impact of different incubation times on the quantity of viable bacteria in final lactobacillus completed product, and find out optimum incubation time.
The incubation time of milk-acid bacteria is unsuitable long, because overlong time, milk-acid bacteria there will be autolysis, causes bacterial number sharply to decline, and therefore, suitable incubation time is most important.According to method of the present invention, adopt single factor design, incubation time is set to respectively to 12h, 18h, 24h, 30h, 36h and 48h, to cultivate, and measure the quantity of viable bacteria in different incubation time gained milk-acid bacterias, it the results are shown in accompanying drawing 3.
From accompanying drawing 3, can find out, when incubation time is 24h ~ 30h, in milk-acid bacteria, number of viable is more, and in the time that incubation time is 30h, it is maximum that its viable count reaches, and therefore according to method of the present invention, optimum incubation time is 30h.
Claims (6)
1. utilize alcohol industry yellow water to produce a method for milk-acid bacteria, it is characterized in that: comprise the following steps:
(1) the alcohol industry yellow water of collecting is filtered, and it is for subsequent use to get its filtrate;
(2) the alcohol industry yellow water filtrate of step (1) gained is carried out to the mensuration of nutritive ingredient, and according to measurement result, alcohol industry yellow water filtrate is carried out to nutritional supplementation, for subsequent use;
(3) the alcohol industry yellow water filtrate after nutritional supplementation in step (2) is carried out to sterilizing, and by pH regulator to 6.3 ~ 6.5 of the alcohol industry yellow water filtrate after sterilizing, for subsequent use;
(4) lactic acid bacteria culturers is inoculated on MRS liquid nutrient medium, and is cultured to the logarithmic growth middle and later periods, detect viable count and be not less than 1 × 10
8for subsequent use as kind of daughter bacteria liquid when cfu/mL;
(5) according to 5%(v/v) ratio by step (4) gained kind daughter bacteria liquid be inoculated in the alcohol industry yellow water filtrate of step (3) gained, and cultivate 24 ~ 30h under the condition of 37 ~ 37.5 DEG C;
(6) in step (5), in the milk-acid bacteria of cultivation, add 2% cooked rice flour noodles according to weightmeasurement ratio, and leave standstill 5min-10min under room temperature, then by the centrifugal milk-acid bacteria solid mixture that obtains, for subsequent use;
(7) the milk-acid bacteria solid mixture of gained in step (6) is spread out into the thickness of 0.3 ~ 0.5cm, be placed in vacuum freeze drier and be dried, after being dried, carry out vacuum packaging, obtain lactobacillus completed product.
2. a kind of method of utilizing alcohol industry yellow water to produce milk-acid bacteria according to claim 1, is characterized in that: the alcohol industry yellow water in step (1) is to collect and obtain from the sheet frame of plate-and-frame filter press.
3. a kind of method of utilizing alcohol industry yellow water to produce milk-acid bacteria according to claim 2, it is characterized in that: the sterilising method in step (3) is first by pH regulator to 3.2 ~ 3.5 of the alcohol industry yellow water filtrate after supplementing the nutrients, then to injecting carbon dioxide gas in the container of splendid attire alcohol industry yellow water filtrate, sealing room temperature are placed 24h.
4. a kind of method of utilizing alcohol industry yellow water to produce milk-acid bacteria according to claim 3, it is characterized in that: the preparation method of the MRS liquid nutrient medium in step (4) is: get beef protein powder 10g, beef extract 10g, yeast extract 5g, glucose 20g, sodium-acetate 5g, dibasic ammonium citrate 2g, tween 80 0.1mL, magnesium sulfate 0.58g, manganous sulfate 0. 28g and aquae destillata 1000mL and mix, and with high-pressure sterilizing pot at 121 DEG C of sterilizing 20min-30min, then regulate pH to 6.3 ~ 6.5.
5. a kind of method of utilizing alcohol industry yellow water to produce milk-acid bacteria according to claim 4; it is characterized in that: carrying out step (7) before; according to the weight of the milk-acid bacteria solid mixture of gained in step (6);, and mix to adding in milk-acid bacteria solid mixture the cycloheptaamylose of 2.5% ~ 3% sodium alginate, 4.5% ~ 5% skim-milk, 5% glycerine and 8% ~ 10% as protective material according to weight ratio.
6. a kind of alcohol industry yellow water that utilizes according to claim 5 is produced the method for milk-acid bacteria, it is characterized in that: the temperature of the vacuum freeze drier described in step (7) is-45 DEG C ~-50 DEG C, vacuum tightness 10 ~ 20Pa, time of drying 22 ~ 24h.
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| CN104543402A (en) * | 2015-01-20 | 2015-04-29 | 河南科技大学 | Preparation method of fermentation-state iron feed additive |
| CN104543398A (en) * | 2015-01-20 | 2015-04-29 | 河南科技大学 | Preparation method of fermentation state copper feed additive |
| CN104543400A (en) * | 2015-01-20 | 2015-04-29 | 河南科技大学 | Preparation method of fermentation state zinc feed additive |
| CN104543400B (en) * | 2015-01-20 | 2018-04-20 | 河南科技大学 | A kind of preparation method for the state zinc feed additive that ferments |
| CN106497732A (en) * | 2016-12-08 | 2017-03-15 | 彭万洪 | A kind of method that yellow water safeguards pit mud |
| CN106591041A (en) * | 2016-12-08 | 2017-04-26 | 彭万洪 | Yellow water reutilization method |
| CN106665837A (en) * | 2016-12-16 | 2017-05-17 | 常州市鼎日环保科技有限公司 | Preparation method of high-activity ready-to-use yoghurt starter |
| CN107996942A (en) * | 2017-12-01 | 2018-05-08 | 阳江利洋苗种繁育有限公司 | A kind of fairy shrimp nauplius preserving agent and its method for preserving |
| CN108865922A (en) * | 2018-05-02 | 2018-11-23 | 山东出入境检验检疫局检验检疫技术中心 | listeria monocytogenes standard sample and preparation method thereof |
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Application publication date: 20140827 |