CN104543402A - Preparation method of fermentation-state iron feed additive - Google Patents
Preparation method of fermentation-state iron feed additive Download PDFInfo
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Abstract
The invention discloses a preparation method of a fermentation-state iron feed additive. The method comprises the following steps: firstly, by adopting industrial and agricultural by-products such as alcohol industry yellow water, bran and powder dregs as raw materials, preparing a fermentation medium; carrying out microbiological screening and domesticated incubation to obtain iron-rich microorganisms, and inoculating the microorganisms into the obtained fermentation medium and fermenting; and drying, crushing and packaging the fermented material to prepare the product. Compared with chemical synthesized iron feed additives, the fermentation-state iron feed additive has the advantages that the by-products such as powder dregs, bran and alcohol industry yellow water are adopted as culture raw materials and mediums, so that resource utilization is expanded; the production cost of the iron feed additive is reduced; the prepared fermentation state iron feed additive is relatively stable in bio-availability, and relatively high in absorptivity; and the iron absorptivity is greater than 24.7%, is 5.7 times that of ferrous sulfate monohydrate, and is 1.8 times that of iron amino acid chelate.
Description
Technical field
The present invention relates to a kind of biological feedstuff manufacture field, especially utilize microorganism to prepare the method for fermentation state iron feed additive.
Background technology
Iron is one of required trace quantity mineral substance element of animal body, and iron deficiency is more common in children livestock and poultry in age, breeding domestic animal etc.Iron deficiency can cause breeding performonce fo animals to decline, and resistance reduces, anaemia etc. time serious.Therefore, to add iron preparation be the important measures meeting animal body iron needs by extra in feed.But research finds, the biological effectiveness of inorganic states iron preparation (as ferrous sulfate, frerrous chloride etc.) is lower, and its absorption rate is lower than 10%, and all the other Tie Tongs cross ight soil and enter environment, pollute.Research in recent years finds, the utilization rate of the organic iron preparations such as organic chelated state iron preparation, heme iron, animal fodder iron is significantly higher than inorganic states iron.This has evoked the interest that researchers study organic iron preparation greatly, and some organic iron preparations are also constantly developed, as ironic citrate, glucoferrum, EDTA-FeNa, amino acid chelated iron etc.But along with going deep into of research, find that its utilization rate of organic iron of Prof. Du Yucang is also unstable, some researchs even find, the utilization rate of organic iron preparation it would be better the height of ferrous sulfate.
Microorganism is extensively present in natural organism, and in the last few years, some are of value to human body, the microbe species of animal body is found, and is developed to health products, medicine, feed addictive etc.
The present invention screens available microorganism from the microorganism that No. 1126, The Ministry of Agriculture of the People's Republic of China, MOA bulletin " feed addictive kind catalogue (2008) " approval uses, and by domestication cultivation, fermentation parameter screening etc., development has the fermentation state iron feed additive of higher absorption utilization rate.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of the state iron feed additive that ferments, the strong microorganism fungus kind of rich iron ability is obtained by cultivating the screening of microorganism and domestication, and adopt alcohol industry yellow water, the works such as wheat bran, agricultural by-products is as microbiological culture media matter, carry out the preparation of fermentation state iron feed additive, the absorption rate of iron in effective raising feed addictive, and greatly reduce the production cost of organic iron feed additive, reach resource reutilization simultaneously, reduce the objects such as environmental pollution, and solve the determination of parameters and the drying of fermentation materials in preparation process, the technical problems such as packaging.
The present invention solves the problems of the technologies described above adopted technical scheme:
A kind of preparation method of the state iron feed additive that ferments, first, solid material and liquid charging stock are mixed to get fermentation substrate with the ratio of 1:1 ~ 2, microbe inoculation ferments wherein again, the dry materials, the packaging i.e. obtained product that to obtain after fermentation, wherein said microorganism is that concrete preparation method comprises the following steps through the strong microorganism of the rich iron ability of screening and domestication cultivation obtains:
1, the preparation of fermentation raw material
Described fermentation raw material comprises solid material and liquid charging stock, solid material is wherein according to percentage by weight, it consists of: iron material 5.67%, granulated slag 27.5%, wheat bran 66.5%, urea 0.12%, sugar 0.15%, sodium acid carbonate 0.05%, zinc 0.01%, wherein said iron is divalent state iron, and zinc is inorganic zinc; Described liquid charging stock is the alcohol industry yellow water directly collected from the sheet frame of plate and frame filter press, then after filtration, utilize sodium acid carbonate or NaOH that pH is adjusted to 7.2 ~ 7.5 after sterilizing to obtain, described sterilizing methods is: by filter after alcohol industry yellow water in high-pressure sterilizing pot under 121 DEG C of conditions sterilizing 15 ~ 20min, cooling.
2, microorganism screening and domestication cultivate
2.1, preparation is cultivated and iron content gradient media containing iron base
The method for making of iron content basal medium is: beef extract 5g, peptone 10g, sodium chloride 5g, iron 100mg to be dissolved in distilled water and to be settled to 1L, then adjusting pH to 7.2 ~ 7.5, for subsequent use;
The method for making of iron content gradient media is: according to the method for making of above-mentioned iron content basal medium, iron content is increased progressively 10% successively on the basis of 100mg, and the cumulative volume of culture medium is 1L, configures gradient media successively for subsequent use;
2.2, the screening of microorganism
By the microorganism in No. 1126, The Ministry of Agriculture of the People's Republic of China, MOA bulletin " feed addictive kind catalogue (2008) " according to 5 ~ 8% inoculum concentration, be seeded in the iron content basal medium prepared by step 2.1 respectively, and an iron content basal medium inoculates a kind of microorganism, 36.5 ~ 37.2 DEG C, cultivate 18h in the constant incubator of 55r/min after, record various micro organism quantity, the screened microorganism fungus kind as fermentation of the maximum person of quantity;
2.3, the domestication of microorganism is cultivated
Under the condition of culture same with step 2.2, microorganism fungus kind step 2.2 screened is incubated in iron content gradient media prepared in step 2.1 successively, then the iron content in microorganism is measured, iron content height person is that rich iron ability is strong, thus obtain the strong microorganism fungus kind of rich iron ability, for subsequent use.
3, the inoculation fermentation of microorganism
3.1, the expansion of microorganism fungus kind is cultivated
The microorganism fungus kind that the rich iron ability screening of microorganism in step 2 and domestication cultivation obtained is strong is inoculated in the alcohol industry yellow water after process to be cultivated, its inoculum concentration is 5 ~ 8%, cultivation temperature 36.5 ~ 37.2 DEG C, incubation time 18h, thus the microorganism fungus kind liquid obtained for fermenting, for subsequent use;
3.2, fermentation substrate is prepared
Step 3.1 of learning from else's experience expands the microorganism fungus kind liquid cultivating gained and carries out viable bacteria inspection, when viable count reaches 1 × 10
9during cfu/mL, it is mixed in the mixed solid material of step 1 according to the ratio that weight ratio is 5 ~ 8%, and add the alcohol industry yellow water handled by step 1 according to the ratio of solid-to-liquid ratio 1:1.5, stir and evenly mix, then adjust pH to 7.2 ~ 7.5 and obtain fermentation substrate;
3.3, the fermentation of fermentation substrate
The fermentation substrate of 3.2 gained is placed in the interior fermentation of the constant incubator 22 ~ 24h of 36.5 ~ 37.2 DEG C, obtains fermentation materials.
4, the drying of fermentation materials and packaging
The fermentation materials of gained in step 3.3 is placed in 65 DEG C of Constant Temp. Ovens, and continuous turning over materials, at interval of half an hour, sampling and measuring water content is carried out to material, be down to 12% ~ 14% to material moisture and be drying, then dried material is taken out and pulverize, thus obtain fermentation state iron feed additive finished product, and vacuum packaging, vacuum is 0.085 ~ 0.095Mpa.
Wherein, described iron material can be the divalent state iron such as ferrous sulfate monohydrate, five aqueous ferrous sulfates, ferrous sulfate heptahydrate, frerrous chloride, and by searching the method determination iron content of the description of product or experimental determination.
Described sodium acid carbonate is pure for analyzing, and described zinc is the inorganic zinc such as zinc sulfate, zinc chloride.
Compared with prior art, the invention has the beneficial effects as follows:
(1) compared with the organic iron feed additive of chemical synthesis, the present invention adopts the accessory substances such as granulated slag, wheat bran, alcohol industry yellow water as cultivation raw material and matrix, thus expands the utilization of resources, reduces the production cost of iron feed additive;
(2) compared with the organic iron feed additive of chemical synthesis, fermentation state iron feed additive biological effectiveness prepared by the present invention is more stable, absorptivity is higher, the research of Caco-2 epithelial cell absorbing model is adopted to find, its absorptivity is more than 24.7%, and the absorptivity of current inorganic ferrous sulfate (water) is 4.33%, amino acid chelated iron (compound amino acid, commercially available) absorptivity be 13.6%, therefore, the fermentation state iron feed additive iron absorptivity that prepared by the present invention is 5.7 times and 1.8 times of ferrous sulfate monohydrate and amino acid chelated iron respectively;
(3) the present invention is by screening microorganism and taming cultivation, has more specific aim, improves the efficiency of fermentable, and ensure that in fermentation state iron feed additive to have higher iron absorptivity;
(4) providing a kind of is the serial of methods step that matrix makes fermentation state iron feed additive based on the works such as alcohol industry yellow water, wheat bran, agricultural by-products, comprises solid material content and component proportion, cultivates the regulation and control of material chemical property, fermentation parameter screening etc.
Detailed description of the invention
Below by specific embodiment and relevant test example, the present invention is further illustrated.
Embodiment 1: a kind of preparation method of the state iron feed additive that ferments, comprise the preparation of fermentation raw material, the screening of microorganism and domestication cultivation, the inoculation fermentation of microorganism and the drying of fermentation materials and packaging, described preparation method comprises following concrete steps:
1, the preparation of fermentation raw material
Described fermentation raw material comprises solid material and liquid charging stock.
Solid material comprises iron material, granulated slag, wheat bran, urea and as the sugar of nutritious supplementary pharmaceutical, sodium acid carbonate and zinc, and wherein, iron material is ferrous sulfate monohydrate, and by searching the method determination iron content of the description of product or experimental determination; Granulated slag is potato granulated slag, air bells dry basis, crude protein content 22.5%; Wheat bran is wheat bran, air bells dry basis, crude protein content 15.3%; Urea is commercially available product, uses front requirement not lump, not deliquescence; Sugar can be white granulated sugar, brown sugar, molasses etc.; Sodium acid carbonate is pure for analyzing; Zinc is zinc sulfate.According to the percentage by weight of solid material, carried out by above-mentioned solid material mixing for subsequent use, its concrete ratio is: iron material (in iron) 5.67%, granulated slag 27.5%, wheat bran 66.5%, urea 0.12%, sugar 0.15%, sodium acid carbonate 0.05%, zinc (in zinc) 0.01%.
Described liquid charging stock is the alcohol industry yellow water directly collected from the sheet frame of plate and frame filter press, filtration, sterilizing in the container of sealing, then after utilizing sodium acid carbonate or NaOH that pH is adjusted to 7.2, putting into refrigerator stores for subsequent use, wherein, described sterilizing methods is: by alcohol industry yellow water 121 DEG C of sterilizing 15min in high-pressure sterilizing pot, cooling.
2, microorganism screening and domestication cultivate
2.1, preparation is cultivated and iron content gradient media containing iron base
The method for making of iron content basal medium is: beef extract 5g, peptone 10g, sodium chloride 5g, iron 100mg are dissolved in distilled water, and are settled to 1L, then adjusts pH to 7.2, for subsequent use;
The method for making of iron content gradient media is: according to the method for making of above-mentioned iron content basal medium, iron content is increased progressively 10% successively on the basis of 100mg, and the cumulative volume of culture medium is 1L, configures iron gradient media successively for subsequent use;
2.2, the screening of microorganism
By the microorganism in No. 1126, The Ministry of Agriculture of the People's Republic of China, MOA bulletin " feed addictive kind catalogue (2008) ", inoculative proportion according to 5% is seeded in the iron content basal medium prepared by step 2.1 respectively, 36.5 DEG C, cultivate 18h in the constant incubator of 55r/min after, record various micro organism quantity, the screened microorganism fungus kind as fermentation of the maximum person of quantity, through measuring and calculating, the quantity of bacillus subtilis is maximum, screenedly uses microorganism fungus kind as fermentation.
2.3, the domestication of microorganism is cultivated
Under same condition of culture, bacillus subtilis step 2.2 screened is incubated in iron content gradient media prepared in step 2.1 successively, by colony counting method statistics, under certain high iron concentration, bacillus subtilis can the quantity of survival and reproduction, and survival and reproduction quantity bright resistance to iron ability of more speaking more is strong; Simultaneously, centrifugal under 3000r/min, obtain bacillus subtilis, after employing conventional wet disappears and boils, the iron content in bacillus subtilis is measured with icp ms (ICP-MS), iron content is high is rich iron ability by force, thus obtains the strong Bacillus subtilis strain of rich iron ability, for subsequent use.
3, the inoculation fermentation of described microorganism comprises the following steps:
3.1, the expansion of microorganism fungus kind is cultivated
By the Bacillus subtilis strain in step 2.3 after domestication is cultivated according to 5% ratio be inoculated in process in the alcohol industry yellow water of gained through step 1 and cultivate, cultivation temperature 36.5 DEG C, incubation time 18h, thus obtain the Bacillus subtilis strain liquid for fermenting, for subsequent use;
3.2, fermentation substrate is prepared
The Bacillus subtilis strain liquid getting 3.1 gained carries out viable bacteria inspection, when viable count reaches 1 × 10
9during cfu/mL, by its according to 5% ratio be mixed in solid material prepared by step 1, and add the alcohol industry yellow water handled by step 1 according to the ratio of solid-to-liquid ratio 1:1.5, stir and evenly mix, then adjust pH to 7.2 and obtain fermentation substrate;
3.3, the fermentation of fermentation substrate
Ferment in the constant incubator fermentation substrate of 3.2 gained being placed in 36.5 DEG C 22h, obtains fermentation materials.
4, the drying of fermentation materials and packaging
The fermentation materials of gained in step 3.3 is placed in 65 DEG C of Constant Temp. Ovens, and continuous turning over materials, sampling and measuring water content is carried out at interval of half an hour, until material moisture loss is drying to 12%, then dried material is taken out and pulverize, and cross 80 mesh sieves, thus obtain fermentation state zinc feed additive finished product, in units of 1000g, carry out vacuum packaging with laminated film, vacuum is 0.085Mpa.
Embodiment 2: a kind of preparation method of the state iron feed additive that ferments, comprise the preparation of fermentation raw material, the screening of microorganism and domestication cultivation, the inoculation fermentation of microorganism and the drying of fermentation materials and packaging, described preparation method comprises the steps:
1, the preparation of fermentation raw material
Described fermentation raw material comprises solid material and liquid charging stock.
Solid material comprises iron material, granulated slag, wheat bran, urea and as the sugar of nutritious supplementary pharmaceutical, sodium acid carbonate and zinc, and wherein, iron material is five aqueous ferrous sulfates, and by searching the method determination iron content of the description of product or experimental determination; Granulated slag is potato granulated slag, air bells dry basis, crude protein content 22.5%; Wheat bran is wheat bran, air bells dry basis, crude protein content 15.3%; Urea is commercially available product, uses front requirement not lump, not deliquescence; Sugar can be white granulated sugar, brown sugar, molasses etc.; Sodium acid carbonate is pure for analyzing; Zinc is zinc sulfate.According to the percentage by weight of solid material, carried out by above-mentioned solid material mixing for subsequent use, its concrete ratio is: iron material (in iron) 5.67%, granulated slag 27.5%, wheat bran 66.5%, urea 0.12%, sugar 0.15%, sodium acid carbonate 0.05%, zinc (in zinc) 0.01%.
Described liquid charging stock is the alcohol industry yellow water directly collected from the sheet frame of plate and frame filter press, filtration, sterilizing in the container of sealing, then after utilizing sodium acid carbonate or NaOH that pH is adjusted to 7.5, putting into refrigerator stores for subsequent use, wherein, described sterilizing methods is: by alcohol industry yellow water 121 DEG C of sterilizing 20min in high-pressure sterilizing pot, cooling.
2, microorganism screening and domestication cultivate
2.1, preparation is cultivated and iron content gradient media containing iron base
The method for making of iron content basal medium is: beef extract 5g, peptone 10g, sodium chloride 5g, iron 100mg are dissolved in distilled water, and are settled to 1L, then adjusts pH to 7.5, for subsequent use;
The method for making of iron content gradient media is: according to the method for making of above-mentioned iron content basal medium, iron content is increased progressively 10% successively on the basis of 100mg, and the cumulative volume of culture medium is 1L, configures iron gradient media successively for subsequent use;
2.2, the screening of microorganism
By the microorganism in No. 1126, The Ministry of Agriculture of the People's Republic of China, MOA bulletin " feed addictive kind catalogue (2008) ", inoculative proportion according to 8% is seeded in the iron content basal medium prepared by step 2.1 respectively, 37.2 DEG C, cultivate 18h in the constant incubator of 55r/min after, record various micro organism quantity, the screened microorganism fungus kind as fermentation of the maximum person of quantity, through measuring and calculating, the quantity of bacillus subtilis is maximum, screenedly uses microorganism fungus kind as fermentation.
2.3, the domestication of microorganism is cultivated
Under same condition of culture, bacillus subtilis step 2.2 screened is incubated in iron content gradient media prepared in step 2.1 successively, by colony counting method statistics, under certain high iron concentration, bacillus subtilis can the quantity of survival and reproduction, and survival and reproduction quantity bright resistance to iron ability of more speaking more is strong; Simultaneously, centrifugal under 3000r/min, obtain bacillus subtilis, after employing conventional wet disappears and boils, the iron content in bacillus subtilis is measured with icp ms (ICP-MS), iron content is high is rich iron ability by force, thus obtains the strong Bacillus subtilis strain of rich iron ability, for subsequent use.
3, the inoculation fermentation of described microorganism comprises the following steps:
3.1, the expansion of microorganism fungus kind is cultivated
By the Bacillus subtilis strain in step 2.3 after domestication is cultivated according to 8% ratio be inoculated in process in the alcohol industry yellow water of gained through step 1 and cultivate, cultivation temperature 37.2 DEG C, incubation time 18h, thus obtain the Bacillus subtilis strain liquid for fermenting, for subsequent use;
3.2, fermentation substrate is prepared
The Bacillus subtilis strain liquid getting 3.1 gained carries out viable bacteria inspection, when viable count reaches 1 × 10
9during cfu/mL, by its according to 8% ratio be mixed in solid material prepared by step 1, and add the alcohol industry yellow water handled by step 1 according to the ratio of solid-to-liquid ratio 1:1.5, stir and evenly mix, then adjust pH to 7.5 and obtain fermentation substrate;
3.3, the fermentation of fermentation substrate
Ferment in the constant incubator fermentation substrate of 3.2 gained being placed in 37.2 DEG C 24h, obtains fermentation materials.
4, the drying of fermentation materials and packaging
The fermentation materials of gained in step 3.3 is placed in 65 DEG C of Constant Temp. Ovens, and continuous turning over materials, sampling and measuring water content is carried out at interval of half an hour, until material moisture loss is drying to 14%, then dried material is taken out and pulverize, and cross 80 mesh sieves, thus obtain fermentation state zinc feed additive finished product, in units of 1000g, carry out vacuum packaging with laminated film, vacuum is 0.095Mpa.
Embodiment 3: a kind of preparation method of the state iron feed additive that ferments, comprise the preparation of fermentation raw material, the screening of microorganism and domestication cultivation, the inoculation fermentation of microorganism and the drying of fermentation materials and packaging, described preparation method comprises following concrete steps:
1, the preparation of fermentation raw material
Described fermentation raw material comprises solid material and liquid charging stock.
Solid material comprises iron material, granulated slag, wheat bran, urea and as the sugar of nutritious supplementary pharmaceutical, sodium acid carbonate and zinc, and wherein, iron material is frerrous chloride, and by searching the method determination iron content of the description of product or experimental determination; Granulated slag is potato granulated slag, air bells dry basis, crude protein content 22.5%; Wheat bran is wheat bran, air bells dry basis, crude protein content 15.3%; Urea is commercially available product, uses front requirement not lump, not deliquescence; Sugar can be white granulated sugar, brown sugar, molasses etc.; Sodium acid carbonate is pure for analyzing; Zinc is zinc chloride.According to the percentage by weight of solid material, carried out by above-mentioned solid material mixing for subsequent use, its concrete ratio is: iron material (in iron) 5.67%, granulated slag 27.5%, wheat bran 66.5%, urea 0.12%, sugar 0.15%, sodium acid carbonate 0.05%, zinc (in zinc) 0.01%.
Described liquid charging stock is the alcohol industry yellow water directly collected from the sheet frame of plate and frame filter press, filtration, sterilizing in the container of sealing, then after utilizing sodium acid carbonate or NaOH that pH is adjusted to 7.3, putting into refrigerator stores for subsequent use, wherein, described sterilizing methods is: by alcohol industry yellow water 121 DEG C of sterilizing 18min in high-pressure sterilizing pot, cooling.
2, microorganism screening and domestication cultivate
2.1, preparation is cultivated and iron content gradient media containing iron base
The method for making of iron content basal medium is: beef extract 5g, peptone 10g, sodium chloride 5g, iron 100mg are dissolved in distilled water, and are settled to 1L, then adjusts pH to 7.3, for subsequent use;
The method for making of iron content gradient media is: according to the method for making of above-mentioned iron content basal medium, iron content is increased progressively 10% successively on the basis of 100mg, and the cumulative volume of culture medium is 1L, configures iron gradient media successively for subsequent use;
2.2, the screening of microorganism
By the microorganism in No. 1126, The Ministry of Agriculture of the People's Republic of China, MOA bulletin " feed addictive kind catalogue (2008) ", inoculative proportion according to 7% is seeded in the iron content basal medium prepared by step 2.1 respectively, 36.8 DEG C, cultivate 18h in the constant incubator of 55r/min after, record various micro organism quantity, the screened microorganism fungus kind as fermentation of the maximum person of quantity, through measuring and calculating, the quantity of bacillus subtilis is maximum, screenedly uses microorganism fungus kind as fermentation.
2.3, the domestication of microorganism is cultivated
Under same condition of culture, bacillus subtilis step 2.2 screened is incubated in iron content gradient media prepared in step 2.1 successively, by colony counting method statistics, under certain high iron concentration, bacillus subtilis can the quantity of survival and reproduction, and survival and reproduction quantity bright resistance to iron ability of more speaking more is strong; Simultaneously, centrifugal under 3000r/min, obtain bacillus subtilis, after employing conventional wet disappears and boils, the iron content in bacillus subtilis is measured with icp ms (ICP-MS), iron content is high is rich iron ability by force, thus obtains the strong Bacillus subtilis strain of rich iron ability, for subsequent use.
3, the inoculation fermentation of described microorganism comprises the following steps:
3.1, the expansion of microorganism fungus kind is cultivated
By the Bacillus subtilis strain in step 2.3 after domestication is cultivated according to 7% ratio be inoculated in process in the alcohol industry yellow water of gained through step 1 and cultivate, cultivation temperature 36.8 DEG C, incubation time 18h, thus obtain the Bacillus subtilis strain liquid for fermenting, for subsequent use;
3.2, fermentation substrate is prepared
The Bacillus subtilis strain liquid getting 3.1 gained carries out viable bacteria inspection, when viable count reaches 1 × 10
9during cfu/mL, by its according to 7% ratio be mixed in solid material prepared by step 1, and add the alcohol industry yellow water handled by step 1 according to the ratio of solid-to-liquid ratio 1:1.5, stir and evenly mix, then adjust pH to 7.3 and obtain fermentation substrate;
3.3, the fermentation of fermentation substrate
Ferment in the constant incubator fermentation substrate of 3.2 gained being placed in 36.8 DEG C 23h, obtains fermentation materials.
4, the drying of fermentation materials and packaging
The fermentation materials of gained in step 3.3 is placed in 65 DEG C of Constant Temp. Ovens, and continuous turning over materials, sampling and measuring water content is carried out at interval of half an hour, until material moisture loss is drying to 13%, then dried material is taken out and pulverize, and cross 80 mesh sieves, thus obtain fermentation state zinc feed additive finished product, in units of 1000g, carry out vacuum packaging with laminated film, vacuum is 0.09Mpa.
Test example: adopt the research of Caco-2 epithelial cell absorbing model to find, the iron absorptivity of the fermentation state iron feed additive prepared by the present invention is more than 24.7%, inorganic ferrous sulfate (water) absorptivity is 4.33%, amino acid chelated iron (compound amino acid, commercially available) be 13.6%, therefore, the fermentation state iron feed additive iron absorptivity that prepared by the present invention is 5.7 times and 1.8 times of ferrous sulfate monohydrate and amino acid chelated iron.
Claims (4)
1. the preparation method of state iron feed additive of fermenting, first, solid material and liquid charging stock are mixed to get fermentation substrate with the ratio of 1:1 ~ 2, microbe inoculation ferments wherein again, the dry materials will obtained after fermentation, packaging i.e. obtained product, described liquid charging stock is the alcohol industry yellow water directly collected from the sheet frame of plate and frame filter press, then after filtration, after sterilizing, pH is adjusted to 7.2 ~ 7.5 and obtains, it is characterized in that: described solid material is according to percentage by weight, it consists of: iron 5.67%, granulated slag 27.5%, wheat bran 66.5%, urea 0.12%, sugar 0.15%, sodium acid carbonate 0.05%, zinc 0.01%, wherein said iron is divalent state iron, zinc is inorganic zinc, described microorganism is through the strong microorganism of the rich iron ability of screening and domestication cultivation obtains, screening and the domestication and culture method of microorganism are as follows:
(1) preparation is cultivated and iron content gradient media containing iron base
The method for making of iron content basal medium is: beef extract 5g, peptone 10g, sodium chloride 5g, iron 100mg are dissolved in distilled water, and are settled to 1L, then adjusts pH to 7.2 ~ 7.5, for subsequent use;
The method for making of iron content gradient media is: according to the method for making of above-mentioned iron content basal medium, iron content is increased progressively 10% successively on the basis of 100mg, and the cumulative volume of culture medium is 1L, configures gradient media successively for subsequent use;
(2) screening of microorganism
By the microorganism in No. 1126, The Ministry of Agriculture of the People's Republic of China, MOA bulletin " feed addictive kind catalogue (2008) " according to 5 ~ 8% inoculum concentration, be seeded in the iron content basal medium prepared by step (1) respectively, and an iron content basal medium inoculates a kind of microorganism, 36.5 ~ 37.2 DEG C, cultivate 18h in the constant incubator of 55r/min after, record various micro organism quantity, the screened microorganism fungus kind as fermentation of the maximum person of quantity;
(3) domestication of microorganism is cultivated
Under the condition of culture same with step (2), the microorganism fungus kind that step (2) is screened is incubated at successively in iron content gradient media prepared in step (1), then iron content in microorganism is measured, iron content height person is that rich iron ability is strong, thus obtain the strong microorganism fungus kind of rich iron ability, for subsequent use.
2. the preparation method of a kind of state iron feed additive that ferments according to claim 1, is characterized in that: the inoculation fermentation method of described microorganism is as follows:
(1) expansion of microorganism fungus kind is cultivated
By cultivate through the screening of microorganism and domestication the strong microorganism fungus kind of the rich iron ability that obtains be inoculated in described process after alcohol industry yellow water in cultivate, its inoculum concentration is 5 ~ 8%, cultivation temperature is 36.5 ~ 37.2 DEG C, incubation time is 18h, thus the microorganism fungus kind liquid obtained for fermenting, for subsequent use;
(2) fermentation substrate is prepared
Viable bacteria inspection is carried out, when viable count reaches 1 × 10 to through expanding the microorganism fungus kind liquid cultivating gained
9during cfu/mL, by its according to 5 ~ 8% inoculum concentration be seeded in mixed solid material, and according to solid-to-liquid ratio 1:1.5 ratio add described alcohol industry yellow water process, stir and evenly mix, then adjustment pH to 7.2 ~ 7.5 obtain fermentation substrate;
(3) fermentation of fermentation substrate
Described fermentation substrate is placed in the interior fermentation of the constant incubator 22 ~ 24h of 36.5 ~ 37.2 DEG C, obtains fermentation materials.
3. the preparation method of a kind of state iron feed additive that ferments according to claim 2, it is characterized in that: carrying out the dry method with packing to the fermentation materials of gained is be placed in by the fermentation materials of gained in 65 DEG C of Constant Temp. Ovens, and continuous turning over materials, at interval of half an hour, sampling and measuring water content is carried out to material, be down to 12% ~ 14% to material moisture and be drying, then dried material is taken out and pulverize, thus obtain fermentation state iron feed additive finished product, and vacuum packaging, vacuum is 0.085 ~ 0.095Mpa.
4. the preparation method of a kind of state iron feed additive that ferments according to claim 1, it is characterized in that: the sterilizing methods of described alcohol industry yellow water be by the alcohol industry yellow water after filtering in high-pressure sterilizing pot under 121 DEG C of conditions sterilizing 15 ~ 20min, cooling.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105146100A (en) * | 2015-08-21 | 2015-12-16 | 河南艾格多科技有限公司 | Yeast fermented iron feed additive production method using beer waste liquid |
| CN111321104A (en) * | 2020-04-22 | 2020-06-23 | 河南岐伯实业有限公司 | Preparation method of compound organic trace element feed additive for laying hens |
| CN111328917A (en) * | 2020-04-22 | 2020-06-26 | 河南科技大学 | Preparation method of organic iron preparation |
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| CN105146100A (en) * | 2015-08-21 | 2015-12-16 | 河南艾格多科技有限公司 | Yeast fermented iron feed additive production method using beer waste liquid |
| CN105146100B (en) * | 2015-08-21 | 2018-09-04 | 河南艾格多科技有限公司 | A method of utilizing beer waste liquid production yeast fermentation state iron feed additive |
| CN111321104A (en) * | 2020-04-22 | 2020-06-23 | 河南岐伯实业有限公司 | Preparation method of compound organic trace element feed additive for laying hens |
| CN111328917A (en) * | 2020-04-22 | 2020-06-26 | 河南科技大学 | Preparation method of organic iron preparation |
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| CN104543402B (en) | 2018-04-20 |
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