CN106635762A - Solid matrix fermentation device and method for applying same - Google Patents
Solid matrix fermentation device and method for applying same Download PDFInfo
- Publication number
- CN106635762A CN106635762A CN201710145807.8A CN201710145807A CN106635762A CN 106635762 A CN106635762 A CN 106635762A CN 201710145807 A CN201710145807 A CN 201710145807A CN 106635762 A CN106635762 A CN 106635762A
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- CN
- China
- Prior art keywords
- solid matrix
- fermenting
- installation
- air
- lyophilizing
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/16—Solid state fermenters, e.g. for koji production
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
Abstract
The invention relates to a solid matrix fermentation device and a method for applying the same. By the aid of the solid matrix fermentation device and the method, the shortcoming of deficiency of post-fermentation drying functions of existing solid matrix fermentation devices can be overcome. The solid matrix fermentation device comprises a shell (1), an upper cover (2), stirring blades (3), a motor (4), a mist spray device (5) and freeze-drying air lead-in devices. The method includes firstly, carrying out feeding and sterilizing; secondly, carrying out stirring and inoculating; thirdly, carrying out fermentation; fourthly, filling the solid matrix fermentation device with freeze-drying air and carrying out drying. The solid matrix fermentation device and the method have the advantages that inoculated solid matrixes can be dried without being shifted out, and accordingly sundry bacteria contamination can be prevented; the activity of inoculated microorganisms can be prevented from being affected in drying procedures, the metabolism speeds of the inoculated microorganisms can be reduced, and the activity of the inoculated microorganisms can be kept for a long time.
Description
Technical field
The present invention relates to a kind of solid fermentation device and its using method.
Background technology
Solid fermentation is referred to not to be had or almost in the presence of no Free water, in the water-insoluble solid state substrate for having certain humidity
In, the process of biological respinse is carried out with one or more microorganism, therefore solid fermentation is biological anti-with gas phase as continuous phase
Answer process.
The adopted installation for fermenting of solid matrix fermentation at present all mainly connects bacterium using atomizing, and which is equal with bacterium is connect
Even the characteristics of；But spraying inevitably adds large quantity of moisture during connecing bacterium, while can also produce in solid matrix sweat
The substantial amounts of moisture of life, in causing the solid matrix for fermenting, free water content is higher, easily causes a little varied bacteria growings, affects product matter
Amount.But the function that existing solid matrix installation for fermenting is dried after not possessing fermentation.
The content of the invention
The present invention is the defect for not possessing functions/drying after fermentation in order to solve existing solid matrix installation for fermenting, and is provided
A kind of solid matrix installation for fermenting and its using method.
Solid matrix installation for fermenting includes that housing, upper lid, stirring vane, motor, sprayer unit and lyophilizing air import dress
Put, stirring vane is connected with machine shaft, sprayer unit is arranged on lid；The lower limb of stirring vane is closely pasted with housing bottom
Close.
The using method of above-mentioned solid matrix installation for fermenting：
First, solid matrix fermented material is added into housing, covers in closure, then heat high temperature sterilize；
2nd, spray inoculation microorganism while stirring solid matrix fermented material；
3rd, controlling technological condition for fermentation carries out solid matrix fermentation；
4th, the lyophilizing air for being passed through sterilizing is dried, that is, complete solid matrix fermentation.
Solid matrix installation for fermenting of the present invention need not will connect the solid matrix after bacterium and remove and be capable of achieving drying, it is to avoid miscellaneous
The pollution of bacterium, and dry run does not affect the activity of microbe inoculation, can reduce the rate of metabolism of microbe inoculation on the contrary,
The activity of the holding microbe inoculation of longer time.
The solid matrix fermentation of the similarly suitable dry inoculation anaerobe of apparatus of the present invention.
Solid matrix installation for fermenting of the present invention does not adopt sealing means, therefore low for the requirement of housing, low manufacture cost；
And have the advantages that simple structure, rate of drying are fast.
In solid matrix installation for fermenting of the present invention, the lower limb of stirring vane is brought into close contact with housing bottom, and whipping process is sent out
Without dead angle inside ferment device, the mixing of solid matrix fermented material is homogeneous, it is thorough to be dried.
The solid matrix prepared by solid matrix installation for fermenting of the present invention, because water content is low, nutrition in solid matrix
Thing concentration is homogeneous, there is no gradient, uniform with fermentation, the growth of thalline, the absorption to nutrient and the secretion of metabolite
Uniform advantage.
Description of the drawings
Fig. 1 is one solid matrix installation for fermenting structural profile schematic diagram of specific embodiment.
Specific embodiment
Technical solution of the present invention is not limited to act specific embodiment set forth below, also including between each specific embodiment
Combination in any.
Specific embodiment one：Present embodiment is illustrated with reference to Fig. 1, present embodiment solid matrix installation for fermenting includes shell
Body 1, upper lid 2, stirring vane 3, motor 4, sprayer unit 5 and lyophilizing air unit, stirring vane 3 are connected with machine shaft
Connect, sprayer unit 5 is arranged on lid 2；The lower limb of stirring vane 3 is brought into close contact with 1 bottom of housing.
Present embodiment solid matrix installation for fermenting is provided without vacuum or high-pressure process in use, so not adopting
Sealing means, the gap that lyophilizing air can be between housing and upper lid after contacting with solid matrix materials overflow.
Specific embodiment two：Difference of the present embodiment from specific embodiment one is：The solid matrix fermentation dress
It is set to vertical.Other are identical with embodiment one.
Specific embodiment three：Difference of the present embodiment from specific embodiment one or two is：The solid matrix is sent out
Ferment device case bottom is arc surface.Other are identical with embodiment one or two.
Specific embodiment four：Difference of the present embodiment from one of specific embodiment one to three is：Lyophilizing air
Gatherer includes air sterilization filter plant, gas compressor and pipeline.Other are identical with one of embodiment one to three.
Specific embodiment five：Difference of the present embodiment from one of specific embodiment one to four is：Housing bottom
It is provided with air inlet.Other are identical with one of embodiment one to four.
Specific embodiment six：Difference of the present embodiment from one of specific embodiment one to five is：The lyophilizing
Air unit and fog-spray nozzle UNICOM；Or air nozzle, lyophilizing air unit and air nozzle UNICOM are set on upper lid；
Or lyophilizing air unit and air inlet UNICOM.Other are identical with one of embodiment one to five.
Specific embodiment seven：Difference of the present embodiment from one of specific embodiment one to six is：Fog-spray nozzle with
Air nozzle is arranged on lid middle section.Other are identical with one of embodiment one to six.
Specific embodiment eight：Difference of the present embodiment from one of specific embodiment one to seven is：Solid matrix
Installation for fermenting has double Shell.Other are identical with one of embodiment one to seven.
Present embodiment solid matrix installation for fermenting has double Shell, with insulation, the effect for heating up or lowering the temperature.
Specific embodiment nine：Using method of the present embodiment for one solid matrix installation for fermenting of specific embodiment：
First, solid matrix fermented material is added into housing, covers in closure, then heat high temperature sterilize；
2nd, spray inoculation microorganism while stirring solid matrix fermented material；
3rd, controlling technological condition for fermentation carries out solid matrix fermentation；
4th, the lyophilizing air for being passed through sterilizing is dried, and just completes solid matrix fermentation, you can subpackage.
Specific embodiment ten：Difference of the present embodiment from specific embodiment nine is：In step 3 sweat
Required aseptic gas are passed through by lyophilizing air unit.Other steps and parameter are identical with embodiment nine.
Present embodiment during step 2 and three from lyophilizing air unit to apparatus of the present invention in import needed for
Aseptic gas, can with holding meanss as malleation, external microbe will not enter device in, do not cause living contaminantses.
Specific embodiment 11：Difference of the present embodiment from specific embodiment nine or ten is：Step 4 is passed through
It is stirred while the lyophilizing air of sterilizing.Other steps and parameter are identical with embodiment nine or ten.
Stirring makes solid matrix form whirlpool, and solid matrix is subsided, rolled by four circumferential centers, so as to fill with lyophilizing air
Tap is touched, and accelerates rate of drying.
Specific embodiment 12：Difference of the present embodiment from specific embodiment nine to ten one is：Housing bottom
It is provided with air inlet；In step 3 sweat from air inlet to solid matrix installation for fermenting in be passed through fermentation needed for aseptic gas
Body.
Claims (10)
1. solid matrix installation for fermenting, solid matrix installation for fermenting include housing (1), upper lid (2), stirring vane (3), motor
(4) and sprayer unit (5), it is characterised in that also including lyophilizing air unit, stirring vane (3) is connected with machine shaft,
Sprayer unit (5) is arranged on lid (2)；The lower limb of stirring vane (3) is brought into close contact with housing (1) bottom.
2. solid matrix installation for fermenting according to claim 1, it is characterised in that the solid matrix installation for fermenting is vertical.
3. solid matrix installation for fermenting according to claim 1 and 2, it is characterised in that the solid matrix installation for fermenting housing
Bottom is arc surface.
4. solid matrix installation for fermenting according to claim 1 and 2, it is characterised in that lyophilizing air unit includes sky
Gas sterilising filtration equipment, gas compressor and pipeline.
5. solid matrix installation for fermenting according to claim 4, it is characterised in that housing bottom is provided with air inlet.
6. solid matrix installation for fermenting according to claim 5, it is characterised in that the lyophilizing air unit and spray
Wu Tou UNICOMs；Or air nozzle, lyophilizing air unit and air nozzle UNICOM are set on upper lid；Or lyophilizing air is imported
Device and air inlet UNICOM.
7. solid matrix installation for fermenting according to claim 6, it is characterised in that fog-spray nozzle is arranged on lid with air nozzle
Middle section.
8. solid matrix installation for fermenting according to claim 6, it is characterised in that solid matrix installation for fermenting has bilayer
Housing.
9. the using method of solid matrix installation for fermenting described in claim 1, it is characterised in that solid matrix installation for fermenting makes
Use method：
First, solid matrix fermented material is added into housing, covers in closure, then heat high temperature sterilize；
2nd, spray inoculation microorganism while stirring solid matrix fermented material；
3rd, controlling technological condition for fermentation carries out solid matrix fermentation；
4th, the lyophilizing air for being passed through sterilizing is dried, that is, complete solid matrix fermentation.
10. solid matrix installation for fermenting using method according to claim 9, it is characterised in that in step 3 sweat
Required aseptic gas are passed through by lyophilizing air unit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710145807.8A CN106635762B (en) | 2017-03-13 | 2017-03-13 | Solid matrix installation for fermenting and its application method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710145807.8A CN106635762B (en) | 2017-03-13 | 2017-03-13 | Solid matrix installation for fermenting and its application method |
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| Publication Number | Publication Date |
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| CN106635762A true CN106635762A (en) | 2017-05-10 |
| CN106635762B CN106635762B (en) | 2017-11-07 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710145807.8A Active CN106635762B (en) | 2017-03-13 | 2017-03-13 | Solid matrix installation for fermenting and its application method |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2470365Y (en) * | 2001-02-28 | 2002-01-09 | 食品工业发展研究所 | Deep fermatation apparatus |
| CN101695257A (en) * | 2009-10-30 | 2010-04-21 | 上海芝草生物技术有限公司 | Solid fermentation method for cordyceps sinensis |
| CN102443535A (en) * | 2011-05-10 | 2012-05-09 | 中国科学院过程工程研究所 | Solid fermentation bioreactor |
| CN104004623A (en) * | 2014-06-09 | 2014-08-27 | 泰安生力源生物工程有限公司 | Mass and heat transfer improvement method of solid-state fermentation substrate |
-
2017
- 2017-03-13 CN CN201710145807.8A patent/CN106635762B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2470365Y (en) * | 2001-02-28 | 2002-01-09 | 食品工业发展研究所 | Deep fermatation apparatus |
| CN101695257A (en) * | 2009-10-30 | 2010-04-21 | 上海芝草生物技术有限公司 | Solid fermentation method for cordyceps sinensis |
| CN102443535A (en) * | 2011-05-10 | 2012-05-09 | 中国科学院过程工程研究所 | Solid fermentation bioreactor |
| CN104004623A (en) * | 2014-06-09 | 2014-08-27 | 泰安生力源生物工程有限公司 | Mass and heat transfer improvement method of solid-state fermentation substrate |
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| Publication number | Publication date |
|---|---|
| CN106635762B (en) | 2017-11-07 |
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Effective date of registration: 20210423 Address after: 150028 level 1, unit 2, enterprise accelerator, building 2, innovation and entrepreneurship Plaza, science and technology innovation city, Harbin high tech Industrial Development Zone, Heilongjiang Province Patentee after: Heilongjiang hexufeng Ecological Technology Co.,Ltd. Address before: 2302, room 105, 150001 postal street, Nangang District, Heilongjiang, Harbin Patentee before: Wen Tiejun |
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