CN106635378A

CN106635378A - Method for extracting fruit oil from fructus hippophae puree

Info

Publication number: CN106635378A
Application number: CN201710085840.6A
Authority: CN
Inventors: 吴相如; 陈敏; 闫鑫; 郭文娟; 李琼
Original assignee: INST OF FOOD INDUSTRY SHANXI PROV; Shanxi Lead Biotechnology Development Co Ltd
Current assignee: INST OF FOOD INDUSTRY SHANXI PROV; Shanxi Lead Biotechnology Development Co Ltd
Priority date: 2017-02-17
Filing date: 2017-02-17
Publication date: 2017-05-10

Abstract

The invention relates to food processing and brewing and in particular relates to a method for extracting fruit oil from fructus hippophae puree. The method for extracting the fruit oil from the fructus hippophae puree comprises the following steps: step 1, adding compound pectinase which is 0.005 percent to 0.01 percent of the mass of the fructus hippophae puree into the fructus hippophae puree; carrying out enzymolysis for 30min at a temperature of 45 DEG C to 50 DEG C; combining centrifuging and separation with super-critical CO2 extraction to separate fructus hippophae fruit oil; step 2, carrying out degumming, deacidification, de-coloring and deodorization treatment on the fructus hippophae fruit oil separated in the step 1. According to the method provided by the invention, the repeated enzymolysis is carried out on the fructus hippophae puree obtained by refining fructus hippophae oil and then the fructus hippophae fruit oil is separated through combining the centrifuging and separation with the super-critical CO2 extraction; the edible fructus hippophae fruit oil is obtained through the degumming, deacidification, de-coloring and deodorization treatment.

Description

A kind of method that fruit oil is extracted from seabuckthorn fruit mud

Technical field

The present invention relates to a kind of method that fruit oil is extracted from seabuckthorn fruit mud.

Background technology

Fructus Hippophae, is the fruit of Elaeangnaceae Hippophae machaka Fructus Hippophae, another name acid thorn, black thorn etc..Fructus Hippophae contains many Nutrient substance is planted, " third generation fruit ", " king of vitamin " is described as and " 21st century, most promising new health care product was former Material ".It is living containing multivitamin, fatty acid, trace element, sub- oil element, Fructus Hippophae flavone, superoxides etc. in its fruit according to surveying and determination The various aminoacid of property material and needed by human body.Fructus Hippophae can be widely applied to food, medicine, light industry, space flight, agriculture and animal husbandry fishery Etc. many fields of national economy.Fructus Hippophae is also processed to the products such as fruit wine, fruit vinegar, fruit juice.Existing Oleum Hippophae is directly needed Directly to extract from Fructus Hippophae, the extraction ratio of this mode is typically relatively low, it is impossible to effectively utilize Fructus Hippophae.

The content of the invention

The technical problem to be solved is：How the oil yield of Oleum Hippophae is improved.

The technical solution adopted in the present invention is：A kind of method that fruit oil is extracted from seabuckthorn fruit mud, according to following step Suddenly carry out

Step one, the compound pectinase for adding in seabuckthorn fruit mud its quality 0.05-0.1 ‰, digest at a temperature of 45-50 DEG C 30min, by centrifugation and supercritical CO₂Extraction phase is combined and separates Oleum Hippophae；

Step 2, by the isolated Oleum Hippophae of step one by degumming, deacidification, decolouring, deodorize process.

As a kind of optimal way：Centrifugation and supercritical CO in step one₂Extraction phase is combined and separates Oleum Hippophae Refer to by the seabuckthorn fruit mud after enzymolysis through centrifuge separation after, to separating impurity after Oleum Hippophae pass through supercritical CO₂Liquid Body is extracted, and further removes Oleum Hippophae and supercritical CO₂Solid impurity in mixed liquid, then Oleum Hippophae and super Critical CO₂Mixed liquid blood pressure lowering makes CO₂Liquid is changed into gas and discharges so as to be left Oleum Hippophae.

As a kind of optimal way：Degumming in step 2 is referred to and is heated to the isolated Oleum Hippophae of step one 60-80 DEG C, the mass percent concentration for adding its quality 0.2%-0.3% is 50% citric acid, adds Oleum Hippophae and Fructus Citri Limoniae The water mixing of sour mixed liquor quality 2%-5%, stirs 20-50min, is then separated using centrifuge and removes pectin.

As a kind of optimal way：Deacidification in step 2 is referred to and will be heated to 50- through the Oleum Hippophae of degumming process 70 DEG C, mass percent concentration is added for the alkali liquor of 10%-15%, excess alkali quantity 0.2%-0.4%, centrifugation point after stirring reaction 20min From, isolated Oleum Hippophae is heated to into 60-80 DEG C, after adding 80-85 DEG C of hot water stirs uniform, centrifugation removes useless Water, Deca phenolphthalein in waste water continuously adds in detached Oleum Hippophae 80-85 DEG C of hot water stirs equal if solution changes color After even, centrifugation removes waste water, until solution invariant color after Deca phenolphthalein in waste water.

As a kind of optimal way：Decolouring in step 2 refers to and its matter is added in the Oleum Hippophae that will be processed through deacidification The activated carbon of amount 3%-5%, stirring is warmed up to 155-165 DEG C, reacts 20min, and centrifugation after cooling to less than 70 DEG C must take off The Oleum Hippophae of color, Oleum Hippophae color is changed into faint yellow from original brownish red.

As a kind of optimal way：Deodorize in step 2 is referred to using the vacuum decompression way of distillation to through decolouring Oleum Hippophae carries out deodorize, i.e., being put in hermetic container through the Oleum Hippophae for decolourizing, be passed through nitrogen and exclude air, then takes out To 1-3KPa, 230 ~ 250 DEG C of keeping temperature, deodorization time is 0.3-1.0h to vacuum.

The invention has the beneficial effects as follows：The present invention carries out repeating enzymolysis, Ran Houtong to the seabuckthorn fruit mud for refining Oleum Hippophae Cross centrifugation and supercritical CO₂Extraction phase is combined and separates Oleum Hippophae, then processes acquisition through degumming, deacidification, decolouring, deodorize Edible Oleum Hippophae.

Specific embodiment

Step one, the compound pectinase for adding in the seabuckthorn fruit mud for refined Oleum Hippophae its quality 0.08 ‰, 30min is digested at a temperature of 50 DEG C, by centrifugation and supercritical CO₂Extraction phase is combined and separates Oleum Hippophae, to what is isolated Seabuckthorn fruit mud continuously adds the compound pectinase of its quality 0.08 ‰, and at a temperature of 50 DEG C 30min is digested, by centrifugation and SCF-CO 2 combines separation Oleum Hippophae, in triplicate, all detached Oleum Hippophaes is merged together.Pass through After three enzymolysis, the effective ingredient of Fructus Hippophae is utilized to the full extent, and the impurity after remaining centrifugation is almost fiber.Centrifugation point From and supercritical CO₂Extraction phase combines separation Oleum Hippophae and refers to by the seabuckthorn fruit mud after enzymolysis after centrifuge separation, right Separate Oleum Hippophae after impurity and pass through supercritical CO₂Liquid extraction, further removes Oleum Hippophae and supercritical CO₂Liquid is mixed The solid impurity in liquid is closed, then Oleum Hippophae and supercritical CO₂Mixed liquid blood pressure lowering makes CO₂Liquid is changed into gas discharge So as to be left Oleum Hippophae.Supercritical extraction condition：Supercritical extraction pressure is 25-30Mpa, and extraction temperature is 40-45 DEG C, extraction The time is taken for 2-3 h, CO₂Flow is 10-20L/h.

Step 2, degumming, it is therefore an objective to remove phospholipid, mucus, micro heavy, protein, saccharide and some thermal sensitivity colors Element, by the isolated Oleum Hippophae of step one 60-80 DEG C is heated to, and adds the mass percent of its quality 0.2%-0.3% dense The citric acid for 50% is spent, the water mixing of Oleum Hippophae and citric acid mixed liquor quality 2%-5% is added, 20-50min is stirred, so Separated using centrifuge afterwards and remove pectin, isolated pectin is put in the seabuckthorn fruit mud of step one and digested.Deacidification, adopts Deacidification is carried out with alkali refining method, alkali refining is actually the process of an acid-base neutralization, and ultimate principle is using alkali liquor and free-fat There is the alkali metal salt that neutralization reaction forms fatty acid in acid, so as to separate with oils and fatss, and in acid-base neutralization forming salt precipitation Meanwhile, the phospholipid in oils and fatss, protein, viscose glue, pigment etc. are removed, 50- will be heated to through the Oleum Hippophae of degumming process 70 DEG C, mass percent concentration is added for the alkali liquor of 10%-15%（Sodium hydroxide or potassium hydroxide）, excess alkali quantity 0.2%-0.4%, Centrifugation after stirring reaction 20min, by isolated Oleum Hippophae 60-80 DEG C is heated to, and adds 80-85 DEG C of hot water stirs After uniform, centrifugation removal waste water, Deca phenolphthalein in waste water, continuation adds in detached Oleum Hippophae if solution changes color Enter 80-85 DEG C of hot water stirs it is uniform after, centrifugation remove waste water, until solution invariant color after Deca phenolphthalein in waste water.It is de- Color, is decolourized using charcoal-stripped to Oleum Hippophae, and its quality 3%- is added in the Oleum Hippophae that will be processed through deacidification 5% activated carbon, stirring is warmed up to 155-165 DEG C, reacts 20min, and centrifugation after cooling to less than 70 DEG C must decolourize Oleum Hippophae, Oleum Hippophae color is changed into faint yellow from original brownish red.Deodorize, is put into the Oleum Hippophae through decolourizing close In closing container, it is passed through nitrogen and excludes air, be then evacuated down to 1-3KPa, 230 ~ 250 DEG C of keeping temperature, deodorization time is 0.3-1.0h。

Claims

1. it is a kind of from seabuckthorn fruit mud extract fruit oil method, it is characterised in that：Carry out according to the steps

2. it is according to claim 1 it is a kind of from seabuckthorn fruit mud extract fruit oil method, it is characterised in that：In step Centrifugation and supercritical CO₂Extraction phase combines separation Oleum Hippophae and refers to the seabuckthorn fruit mud after enzymolysis through centrifuge separation Afterwards, to separating impurity after Oleum Hippophae pass through supercritical CO₂Liquid extraction, further removes Oleum Hippophae and supercritical CO₂Liquid Solid impurity in body mixed liquor, then Oleum Hippophae and supercritical CO₂Mixed liquid blood pressure lowering makes CO₂Liquid is changed into gas Discharge so as to be left Oleum Hippophae.

3. it is according to claim 1 it is a kind of from seabuckthorn fruit mud extract fruit oil method, it is characterised in that：In step 2 Degumming refers to and for the isolated Oleum Hippophae of step one to be heated to 60-80 DEG C, adds the quality percentage of its quality 0.2%-0.3% Specific concentration is 50% citric acid, adds the water mixing of Oleum Hippophae and citric acid mixed liquor quality 2%-5%, stirs 20- 50min, is then separated using centrifuge and removes pectin.

4. it is according to claim 1 it is a kind of from seabuckthorn fruit mud extract fruit oil method, it is characterised in that：In step 2 Deacidification is referred to will be heated to 50-70 DEG C through the Oleum Hippophae of degumming process, add the alkali that mass percent concentration is 10%-15% Isolated Oleum Hippophae is heated to 60-80 DEG C by liquid, excess alkali quantity 0.2%-0.4%, centrifugation after stirring reaction 20min, After adding 80-85 DEG C of hot water stirs uniform, centrifugation removes waste water, and Deca phenolphthalein in waste water is dividing if solution changes color From Oleum Hippophae in continuously add 80-85 DEG C of hot water stirs it is uniform after, centrifugation remove waste water, until Deca in waste water Solution invariant color after phenolphthalein.

5. it is according to claim 1 it is a kind of from seabuckthorn fruit mud extract fruit oil method, it is characterised in that：In step 2 Decolouring refers to the activated carbon that its quality 3%-5% is added in the Oleum Hippophae that will be processed through deacidification, stirs, and is warmed up to 155- 165 DEG C, 20min is reacted, the Oleum Hippophae that centrifugation must decolourize after cooling to less than 70 DEG C, Oleum Hippophae color is by original Brownish red is changed into faint yellow.

6. it is according to claim 1 it is a kind of from seabuckthorn fruit mud extract fruit oil method, it is characterised in that：In step 2 Deodorize refers to and deodorize is carried out to the Oleum Hippophae through decolourizing using using the vacuum decompression way of distillation, i.e., the Fructus Hippophae through decolourizing Fruit oil is put in hermetic container, is passed through nitrogen and is excluded air, is then evacuated down to 1-3KPa, 230 ~ 250 DEG C of keeping temperature, deodorize Time is 0.3-1.0h.