CN110201043A - A kind of olibanum composite essential oil and application - Google Patents
A kind of olibanum composite essential oil and application Download PDFInfo
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- CN110201043A CN110201043A CN201910461835.XA CN201910461835A CN110201043A CN 110201043 A CN110201043 A CN 110201043A CN 201910461835 A CN201910461835 A CN 201910461835A CN 110201043 A CN110201043 A CN 110201043A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/32—Burseraceae (Frankincense family)
- A61K36/324—Boswellia, e.g. frankincense
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/738—Rosa (rose)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/022—Refining
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/025—Recovery by solvent extraction
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/027—Recovery of volatiles by distillation or stripping
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/31—Extraction of the material involving untreated material, e.g. fruit juice or sap obtained from fresh plants
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Abstract
The invention discloses a kind of olibanum composite essential oil and applications, and wherein olibanum composite essential oil includes olibanum essential oil, Rosa Damascana, Rosemary Oil, evening primrose oil, base oil；Using include olibanum composite essential oil preparation treatment pigmentation disease drug in application, olibanum composite essential oil preparing the application in skin-whitening and nti-freckle health-oriented products.Present invention firstly discovers that preparation, which has, inhibits melanin activity and its in preparation whitening and nti-freckle health-oriented products or skin-whitening and the Composite Milk essential oil of the new application for the drug for treating pigmentation disease；The present invention can play effective inhibition and decomposition to melanin, so that melanin spot is thoroughly disappeared, can play the effect of whitening and nti-freckle, and just display is known as stronger inhibiting effect to black at low concentrations；Acute Toxicity of the present invention is low, highly effective and safe, therefore can be used to prepare with whitening and cosmetics or skin-whitening and treat the drug of pigmentation disease.
Description
Technical field
Pigmentation is treated the invention belongs to pharmaceutical technology field more particularly to a kind of olibanum composite essential oil and its in preparation
Application in the drug and skin-whitening and nti-freckle health-oriented products of property disease.
Background technique
The depth of human skin color is regulated and controled by many factors, but content and distribution main or that depend on melanin,
So the whitening idea of people has turned to the physiological whitening of melanin reduction.A large amount of whitening shield is emerged currently on the market
Skin product, safety and Efficiency assessment become consumer and manufacturer emphasis of interest naturally.From animals and plants, Chinese herbal medicine
The natural active matter extracted in equal natural materials, because chemical structure and bioactivity do not change, and it has multiple efficiency,
Lasting medicine is stablized, widely applicable, without side-effects or side effect very little, complies with " back to nature, scientific beauty, section so having
Learn and nurse this trend " the advantages of.
Olibanum composite essential oil is the ratio of genuine milk essential oil, Rosa Damascana, Rosemary Oil and its evening primrose oil by optimization
Example is deployed.It does not find that above-mentioned composite essential oil has before making the present invention and inhibits melanin activity or using it as raw material preparation
The report of cosmetics or pharmaceutical preparation, also not in preparation whitening and cosmetics or skin-whitening and treatment pigmentation
The report of application in the drug of disease.
Summary of the invention
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to provide a kind of olibanum composite essential oil and application.
The purpose of the present invention is achieved through the following technical solutions: a kind of olibanum composite essential oil, by weight percent
Meter, including olibanum essential oil 1%-5%, Rosa Damascana 8-15%, Rosemary Oil 3-8%, evening primrose oil 8-10%, base oil
62-80%.
Further, the base oil is by Sweet Almond Oil, SIMMONDSIA CHINENSIS SEED OIL, grape seed oil, prinsepia utilis royle oil, olive oil, rose
One or more of fruit oil is by any proportion mixing composition.
Further, the olibanum includes the resin of the bark exudation of Burseraceae Boswellia.
Further, the rosemary includes Labiatae Rosmarinus plant.
Further, the rose includes rosaceae rose.
Further, the oenothera biennis includes Oenotheraceae Oenothera plant.
Further, the olibanum essential oil extracts by the following method obtains:
(1) it ferments: choosing Boswellia carterii resin, pile fermentation is placed about 1-2 days, and the coarse granule for being then crushed to 30-60 mesh is spare.
(2) it extracts: being extraction in 5.0-6.0 hydrochloric acid or aqueous sulfuric acid by the pH value that coarse granule is soaked in 7-9 times of quality
Overnight, extraction mixed liquor is obtained.
(3) it digests: cellulase and pectase, cellulase, pectase and extraction mixing being added into extraction mixed liquor
The quality proportioning of liquid is 0.5-1:0.1-0.5:100, is digested about 2-3 hours at 35-37 DEG C.
(4) it extracts: the mixed liquor after step 3 enzymatic hydrolysis being put into steam distillation and is extracted in instrument, 100 DEG C of -120 DEG C of reflux
It extracts about 5-7 hour, collection distillate is cooled to room temperature to be placed in oil water separator and separates, oil reservoir anhydrous sodium sulfate
Dry, separation is to get olibanum essential oil；Or the mixed liquor after step 3 enzymatic hydrolysis is put into CO 2 supercritical and is extracted in instrument, it mentions
The flow velocity of -48 DEG C of the temperature 45 C taken, pressure 13.70MPa-17.80MPa, time 50min-100min, carbon dioxide is
25kg/h-35kg/h；Extract is collected, is placed in molecular distillation instrument and refines, feeding temperature is 40 DEG C -45 DEG C, sample introduction speed
For 480-530mL/h, one section of evaporating temperature is 110 DEG C -115 DEG C, and dual circulation temperature is 60 DEG C -65 DEG C, collects two sections of distillations
Part obtains olibanum essential oil.
Further, the Rosa Damascana, the extracting method of Rosemary Oil are identical, comprising the following steps: put raw material
Enter steam distillation to extract in instrument, the saturated salt solution of 7-9 times of quality is added, 100 DEG C of -120 DEG C of refluxing extractions are 3-5 small
When, collect distillate, be cooled to room temperature to be placed in oil water separator and separate, oil reservoir is dry with anhydrous sodium sulfate, separation to get
Essential oil.The raw material is fresh rose or rosemary；The essential oil is Rosa Damascana or Rosemary Oil.
Further, the evening primrose oil is extracted obtain by the following method: after oenothera seed is crushed to 60-80 mesh,
The lime white that the concentration that 4-8 times of quality is added is 1-2% adjusts pH value and is put into squeezer after impregnating 6-8 hours to 11-13
Squeezing is placed in supercentrifuge, water-oil separating, oil reservoir are placed in molecule by 5000-7000 revs/min after collecting juicing filtering
It being refined in distillation apparatus, feeding temperature is 40 DEG C -45 DEG C, and sample introduction speed is 480-530mL/h, one section of evaporating temperature is 110 DEG C -
115 DEG C, dual circulation temperature is 60 DEG C -65 DEG C, collects one section of distillation fraction, obtains evening primrose oil.
A kind of application of above-mentioned olibanum composite essential oil, the application include olibanum composite essential oil in preparation treatment pigmentation
Application, olibanum composite essential oil in the drug of property disease are preparing the application in skin-whitening and nti-freckle health-oriented products.It is described strong
Health product includes massage oil, cosmetics, showering agent and fragrance fumette.
The beneficial effects of the present invention are: the present invention provides a kind of preparation methods of olibanum composite essential oil, and find for the first time
Olibanum composite essential oil, which has, inhibits melanin activity and its in preparation whitening and nti-freckle health-oriented products or skin-whitening and treatment color
The new application of the drug of the pigmented disease of element.Our experiments show that olibanum composite essential oil of the present invention can play melanin
Out effectively inhibit or (and) decomposition, so that melanin spot is thoroughly disappeared, the effect of whitening and nti-freckle can be played；Olibanum is compound
Essential oil has stronger inhibitory effect to melanin, and just display is known as stronger inhibiting effect to black at low concentrations；
And show that olibanum composite essential oil Acute Toxicity is low through zebra fish acute toxicity test, highly effective and safe, therefore olibanum composite precision
Oil can be used to prepare with whitening and cosmetics or skin-whitening and treat the drug of pigmentation disease.
Detailed description of the invention
Fig. 1 is the pharmacodynamics schematic diagram for inhibiting melanin by image analysis qualitative evaluation olibanum composite essential oil；Wherein,
(a) it is blank control schematic diagram, (b) is 0.1% DMSO solvent control schematic diagram, (c) at 60 μ g/mL olibanum composite essential oils
Reason group schematic diagram (d) is 100 μ g/mL olibanum composite essential oil processing group schematic diagrames, (e) at 200 μ g/mL olibanum composite essential oils
Reason group schematic diagram (f) is 400 μ g/mL olibanum composite essential oil processing group schematic diagrames；
The pharmacodynamics schematic diagram of Fig. 2 histogram analysis olibanum composite essential oil inhibition melanin；
Fig. 3 is to inhibit the pharmacodynamics of melanin to illustrate by the qualitative relatively olibanum composite essential oil of image analysis and arbutin
Figure；Wherein, (a0) it is solvent control group schematic diagram, (a1) it is 60 μ g/mL olibanum composite essential oil processing group schematic diagrames, (a2) it is 100
μ g/mL olibanum composite essential oil processing group schematic diagram, (a3) it is 200 μ g/mL olibanum composite essential oil processing group schematic diagrames, (a4) it is 400
μ g/mL olibanum composite essential oil processing group schematic diagram, (b0) it is blank control group schematic diagram, (b1) it is that 60 μM of arbutin processing groups are shown
It is intended to, (b2) it is 100 μM of arbutin processing group schematic diagrames, (b3) it is 200 μM of arbutin processing group schematic diagrames, (b4) it is 400 μM of bears
Fruit glycosides processing group schematic diagram；
Fig. 4 is to inhibit the pharmacodynamics of melanin to illustrate by image analysis quantitative comparison olibanum composite essential oil and arbutin
Figure.
Specific embodiment
Olibanum composite essential oil of the invention can be any suitable form, such as solid, semisolid, liquid or aerosol shape
Formula.Under normal circumstances, cosmetics or drug contain compound of the invention as active constituent, with suitable outside, enteron aisle or intestines
The organic or inorganic carrier or excipient of stomach external administration mix.Active constituent can be compound, for example, and conventional non-toxic pharmaceutical
Acceptable carriers and/or excipient are made tablet, piller, capsule, suppository, vaginal plug, solution, lotion, suspension and are suitble to
The other forms used.The cosmetics or pharmaceutical acceptable carrier used in the composition include, for example, water, glucose, cream
Sugar, Arabic gum, gelatin, mannitol, starch, magnesium trisilicate, talcum, cornstarch, keratin, colloidal silicon dioxide, horse
Bell sweet potato starch, and it is suitble to other carriers used in the preparation for preparing solid, semisolid, liquid or aerosol form.Combination
In addition object can contain stabilizer, thickener and/or colorant and fragrance.
The present invention is further elaborated with reference to the accompanying drawings and examples, but protection scope of the present invention is not limited to
This.
Embodiment 1:
Take Boswellia carterii resin 150Kg, pile fermentation is placed 2 days, smashes as 50 mesh coarse granules, coarse granule is soaked in 8 times of quality
PH value be 5.0 aqueous hydrochloric acid solutions in, extraction overnight.It is respectively 0.8% fiber that mass percent, which is added, into extraction mixed liquor
Plain enzyme and 0.3% pectase digest 2.5 hours at 37 DEG C.Mixed liquor after enzymatic hydrolysis is put into CO 2 supercritical extraction apparatus
In device, temperature is 45 DEG C, pressure 13.70MPa, time 50min, flow velocity 25kg/h；Extract is steamed by molecule again
Instrument purification is evaporated, feeding temperature is 40 DEG C, and sample introduction speed is 480mL/h, and one section of evaporating temperature is 110 DEG C, dual circulation temperature
It is 60 DEG C, obtains olibanum essential oil；Steam distillation is respectively adopted in fresh rose 1t and rosemary 100Kg to extract, respectively
8 times of quality saturated salt solutions, 100 DEG C of refluxing extractions are added, distillation time 200min collects distillate respectively, is cooled to room temperature
After be respectively placed in oil water separator and separate, oil reservoir is dry with anhydrous sodium sulfate, separation, respectively Rosa Damascana and rosemary essence
Oil；After oenothera seed and dessert almond are crushed to 60-80 mesh respectively, it is separately added into the lime white that the concentration of 5 times of quality is 1.5%
PH value is adjusted to 12, after impregnating 8 hours, is separately added into squeezer and squeezes, after collecting juicing filtering respectively, be respectively placed in high speed
In centrifuge, 6000 revs/min by water-oil separating, oil reservoir is respectively placed in molecular distillation instrument and refines, feeding temperature be 45 DEG C,
Sample introduction speed is 530mL/h, and one section of evaporating temperature is 115 DEG C, and dual circulation temperature is 65 DEG C, collects one section of evaporation section, point
Evening primrose oil, Sweet Almond Oil are not obtained, and by said extracted object olibanum essential oil 5% by mass percentage, Rosa Damascana 15%, fan changes
Essential oil 8% and the Sweet Almond Oil of evening primrose oil 10% and 80% stir evenly mix in proportion, that is, are prepared into olibanum composite essential oil.
Embodiment 2:
Take Boswellia carterii resin 100Kg, pile fermentation is placed 2 days, smashes as 50 mesh coarse granules, coarse granule is soaked in 8 times of quality
PH value be 5.0 aqueous hydrochloric acid solutions in, extraction overnight.It is respectively 0.8% fiber that mass percent, which is added, into extraction mixed liquor
Plain enzyme and 0.3% pectase digest 2.5 hours at 37 DEG C.Mixed liquor after enzymatic hydrolysis is put into CO 2 supercritical extraction apparatus
In device, temperature is 45 DEG C, pressure 13.70MPa, time 50min, flow velocity 25kg/h；Extract is steamed by molecule again
Instrument purification is evaporated, feeding temperature is 40 DEG C, and sample introduction speed is 480mL/h, and one section of evaporating temperature is 110 DEG C, dual circulation temperature
It is 60 DEG C, obtains olibanum essential oil；Steam distillation is respectively adopted in fresh rose 1t and rosemary 100Kg to extract, respectively
8 times of quality saturated salt solutions, 100 DEG C of refluxing extractions are added, distillation time 200min collects distillate respectively, is cooled to room temperature
After be respectively placed in oil water separator and separate, oil reservoir is dry with anhydrous sodium sulfate, separation, respectively Rosa Damascana and rosemary essence
Oil；After oenothera seed and fructus rosae are crushed to 60-80 mesh respectively, it is separately added into the lime white tune that the concentration of 5 times of quality is 1%
PH value is saved to 11, after impregnating 6 hours, is separately added into squeezer and squeezes, after collecting juicing filtering respectively, be respectively placed in high speed from
In scheming, 6500 revs/min by water-oil separating, oil reservoir is respectively placed in molecular distillation instrument and refines, feeding temperature be 45 DEG C, into
Sample speed is 530mL/h, and one section of evaporating temperature is 115 DEG C, and dual circulation temperature is 65 DEG C, collects one section of evaporation section, respectively
Obtain evening primrose oil and rose hip oil.By said extracted object olibanum essential oil 1% by mass percentage, Rosa Damascana 15%, fan changes
Essential oil 3% and the rose hip oil of evening primrose oil 8% and 73% stir evenly mix in proportion, that is, are prepared into olibanum composite essential oil.
Embodiment 3:
Take Boswellia carterii resin 200Kg, pile fermentation is placed 2 days, smashes as 50 mesh coarse granules, coarse granule is soaked in 8 times of quality
PH value be 5.0 aqueous hydrochloric acid solutions in, extraction overnight.It is respectively 0.8% fiber that mass percent, which is added, into extraction mixed liquor
Plain enzyme and 0.3% pectase digest 2.5 hours at 37 DEG C.Mixed liquor after enzymatic hydrolysis is put into CO 2 supercritical extraction apparatus
In device, temperature is 45 DEG C, pressure 13.70MPa, time 50min, flow velocity 25kg/h；Extract is steamed by molecule again
Instrument purification is evaporated, feeding temperature is 40 DEG C, and sample introduction speed is 480mL/h, and one section of evaporating temperature is 110 DEG C, dual circulation temperature
It is 60 DEG C, obtains olibanum essential oil；Steam distillation is respectively adopted in fresh rose 1t and rosemary 100Kg to extract, respectively
8 times of quality saturated salt solutions, 100 DEG C of refluxing extractions are added, distillation time 200min collects distillate respectively, is cooled to room temperature
After be respectively placed in oil water separator and separate, oil reservoir is dry with anhydrous sodium sulfate, separation, respectively Rosa Damascana and rosemary essence
Oil；After oenothera seed and grape pip are crushed to 60-80 mesh respectively, it is separately added into the lime white that the concentration of 5 times of quality is 1.5%
PH value is adjusted to 12, after impregnating 8 hours, is separately added into squeezer and squeezes, after collecting juicing filtering respectively, be respectively placed in high speed
In centrifuge, 6000 revs/min by water-oil separating, oil reservoir is respectively placed in molecular distillation instrument and refines, feeding temperature be 45 DEG C,
Sample introduction speed is 530mL/h, and one section of evaporating temperature is 115 DEG C, and dual circulation temperature is 65 DEG C, collects one section of evaporation section, point
Evening primrose oil, grape seed oil are not obtained, by said extracted object olibanum essential oil 3% by mass percentage, Rosa Damascana 10%, rosemary
Essential oil 5% and the grape seed oil of evening primrose oil 9% and 73% stir evenly mix in proportion, that is, are prepared into olibanum composite essential oil.
Embodiment 4:
Take Boswellia carterii resin 180Kg, pile fermentation is placed 2 days, smashes as 50 mesh coarse granules, coarse granule is soaked in 8 times of quality
PH value be 5.0 aqueous hydrochloric acid solutions in, extraction overnight.It is respectively 0.8% fiber that mass percent, which is added, into extraction mixed liquor
Plain enzyme and 0.3% pectase digest 2.5 hours at 37 DEG C.Mixed liquor after enzymatic hydrolysis is put into CO 2 supercritical extraction apparatus
In device, temperature is 45 DEG C, pressure 13.70MPa, time 50min, flow velocity 25kg/h；Extract is steamed by molecule again
Instrument purification is evaporated, feeding temperature is 40 DEG C, and sample introduction speed is 480mL/h, and one section of evaporating temperature is 110 DEG C, dual circulation temperature
It is 60 DEG C, obtains olibanum essential oil；Steam distillation is respectively adopted in fresh rose 1t and rosemary 100Kg to extract, respectively
8 times of quality saturated salt solutions, 100 DEG C of refluxing extractions are added, distillation time 200min collects distillate respectively, is cooled to room temperature
After be respectively placed in oil water separator and separate, oil reservoir respectively with anhydrous sodium sulfate is dry, separation, respectively Rosa Damascana and fan change
Essential oil；After oenothera seed and green bur are crushed to 60-80 mesh respectively, it is separately added into the stone that the concentration of 5 times of quality is 1.5%
Mortar adjusts pH value to 12, after impregnating 8 hours, is separately added into squeezer and squeezes, after collecting juicing filtering respectively, is respectively placed in
In supercentrifuge, 6000 revs/min by water-oil separating, oil reservoir is respectively placed in molecular distillation instrument and refines, and feeding temperature is
45 DEG C, sample introduction speed is 530mL/h, and one section of evaporating temperature is 115 DEG C, and dual circulation temperature is 65 DEG C, collects one section of evaporation part
Point, evening primrose oil, prinsepia utilis royle oil are obtained respectively.By said extracted object, olibanum essential oil 5%, Rosa Damascana 8% are confused by mass percentage
Repeatedly essential oil 8% and the prinsepia utilis royle oil of evening primrose oil 10% and 69% stir evenly mix in proportion, that is, are prepared into olibanum composite precision
Oil.
Embodiment 5:
The effect that olibanum composite essential oil inhibits zebra fish melanin:
1, zebra fish is chosen:
The AB system zebra fish of 6hpf is placed under disecting microscope and is observed, picking is developed normal and consistent zebra fish and moved into
In 6 hole microwell plates, every 30 tail of hole.(note: the hpf=hour post fertilization in the present invention, Chinese refer to zebra
Fish after fertilization hourage, as 6hpf refers to 6 hours of zebra fish after fertilization).Zebra fish is raised totally-enclosed by Hangzhou Huan Te biology
Circulatory system water cultivating system provides (similarly hereinafter).
2, olibanum compound is handled:
Test four experimental groups of setting, respectively A, B, C and D group, wherein A group is blank control (control) processing group,
Contain 3mL breeding water；B group is solvent (0.1%DMSO, i.e. 0.1% dimethyl sulfoxide) control treatment group, as contains 3 μ L
The 3mL breeding water of DMSO；C group is the arbutin processing group (Arbutin) that concentration is respectively 60,100,200,400,600 μM,
The concentration of the configuration containing useful 3mL breeding water is respectively 60,100,200,400,600 μM of arbutin solution；D group is then concentration
The olibanum composite essential oil processing group of respectively 60,100,200,400,600 μ g/mL, is respectively configured containing useful 3mL breeding water
Concentration is the olibanum composite essential oil solution of 60,100,200,400,600 μ g/mL.Each concentration processing of each experimental group is put
Enter 30 tails and develop consistent 6hpf zebra fish, is placed in 28 DEG C of incubators to be protected from light to cultivate to 52hpf and take pictures.
3, image analysis:
(1) olibanum composite essential oil inhibits the pharmacodynamic evaluation of melanin:
It will be handled by olibanum composite essential oil to the zebra fish of 52hpf (10 tails are chosen in each concentration processing immediately) and be placed in solid
Micro- sem observation is taken pictures (back side sight) and is saved.On the one hand pass through zebra in the observation each processing group of comparative analysis qualitative evaluation
The repressed situation of fish melanin (Fig. 1) visually observes at the olibanum composite essential oil of visible various concentration compared with solvent control
Reason group has different degrees of melanin inhibitory effect；On the other hand using at Nikon NIS-Elements D3.10 high vision
Manage software and quantitative analysis carried out to image, calculate zebra fish rostral posterior opacity average value and olibanum composite essential oil concentration it
Between " drug effect --- dosage " relationship (Fig. 2) and melanin be suppressed rate, compared with solvent control, the olibanum of various concentration is multiple
Essential oil processing group is closed, has significant difference (*: P ﹤ 0.001 of * *) to the inhibitory effect of melanin.Wherein used in quantitative analysis
The term " opacity " arrived, refers to the ratio of opacity summation Yu evaluation region area, opacity summation be evaluated
The melanin content direct proportionality in region.
In addition, inhibiting rate calculation formula of the drug (olibanum composite essential oil, arbutin) to melanin are as follows:
Statistical procedures result withIt indicates, more comparison among groups use variance analysis (ANOVA), and two comparison among groups are adopted
Statistical procedures are carried out with Dunnett ' s T- inspection, p < 0.05 is that otherness is significant.It can be quantified according to statistical procedures result
Determine olibanum composite essential oil to the inhibition situation of melanin.
By quantitative analysis, show that A group blank control group opacity average value is 0.40 ± 0.040；B group solvent control
Group opacity average value is the olibanum composite precision that concentration is respectively 60,100,200,400 μ g/mL in 0.39 ± 0.021, D group
Oil processing group opacity average value difference 0.32 ± 0.041,0.28 ± 0.039,0.22 ± 0.015,0.20 ± 0.019, then
It is obtained according to calculation formula；Concentration is respectively inhibiting rate point of the olibanum composite essential oil to melanin of 60,100,200,400 μ g/mL
Not are as follows: 18.5%, 21.4%, 44.1%, 48.7%, it is seen that the olibanum composite essential oil processing group of various concentration has in various degree
Melanin inhibitory effect.
(2) olibanum composite essential oil is with arbutin compared with inhibiting in melanin effect:
Will by arbutin (C group) and olibanum composite essential oil (D group) processing to 52hpf zebra fish (processing of each concentration with
Choose 10 tails) it is placed in stereoscopic microscope observing, takes pictures (back side sight) and saves.On the one hand by observing, comparative analysis is qualitative to be commented
The repressed situation of zebra fish melanin (Fig. 3) finds the cream of same concentrations by naked eyes qualitative analysis in each processing group of valence
The effect of fragrant composite essential oil processing group inhibition melanin is better than the arbutin processing group of same concentrations；On the other hand Nikon is utilized
NIS-Elements D3.10 high vision processing software carries out quantitative analysis to image, and it is opaque to calculate zebra fish rostral posterior
" drug effect --- the dosage " relationship (Fig. 4) and melanin spent between average value and olibanum composite essential oil concentration are suppressed rate, with
Solvent control is compared, and the olibanum composite essential oil processing group of various concentration has significant difference to the inhibitory effect of melanin；And
Arbutin processing group, in addition to 60 μM of processing groups are compared with solvent control other than no significant difference, other concentration processing groups have
Significant difference；In the olibanum composite essential oil and arbutin processing group of same concentrations, olibanum composite essential oil inhibits the effect of melanin
Fruit will be significantly better than arbutin processing group (*: P ﹤ 0.001 of * *).
In addition, inhibiting rate calculation formula of the drug (olibanum composite essential oil, arbutin) to melanin are as follows:
Statistical procedures result withIt indicates, more comparison among groups use variance analysis (ANOVA), and two comparison among groups are adopted
Statistical procedures are carried out with Dunnett ' s T- inspection, p < 0.05 is that otherness is significant.It can be quantified according to statistical procedures result
Determine olibanum composite essential oil to the inhibition situation of melanin.
By quantitative analysis, show that A group blank control group opacity average value is 0.40 ± 0.040；B group solvent control
Group opacity average value is 0.39 ± 0.021；Concentration is respectively 60,100,200,400 μM of arbutin processing group in C group
Opacity average value is respectively as follows: 0.38 ± 0.044；0.35±0.022；0.32±0.04；0.28±0.043；Concentration in D group
Respectively 60,100,200,400 μM of olibanum composite essential oil processing group opacity average value difference 0.32 ± 0.041,0.28
±0.039,0.22±0.015,0.20±0.019；Then obtained according to calculation formula；Concentration is respectively 60,100,200,400 μM
Arbutin processing group is respectively as follows: 2%, 12.7%, 18.3%, 27% to the inhibiting rate of melanin；And concentration is respectively 60,100,
200, the olibanum composite essential oil of 400 μ g/mL is respectively as follows: 18.5%, 21.4%, 44.1%, 48.7% to the inhibiting rate of melanin.
Zebra fish melanin Inhibition test shows: by 46 hours drug-treateds, same to contrast ratio, arbutin and olibanum were multiple
It closes in essential oil processing group, zebra fish melanin substantially reduces, and skin whitening effects are clearly in quantitative assessment；Arbutin suppression
The action concentration of melanin processed is 100 μM, and olibanum composite essential oil is then 60 μ g/mL；Simultaneously by comparing the bear of same concentrations
Fruit glycosides and olibanum composite essential oil processing group find that the olibanum composite essential oil processing group of each concentration inhibits the effect of melanin obvious
It is better than the arbutin effect of respective concentration.
Embodiment 6:
Zebra fish melanin inhibits to restore test:
1, zebra fish is chosen:
The AB system zebra fish of 6hpf is placed under disecting microscope and is observed, picking is developed normal and consistent zebra fish and moved into
In 6 hole microwell plates, every 30 tail of hole.
2, olibanum compound is handled:
Test three experimental groups of setting, respectively A, B and C group, wherein A group is blank control (control) processing group,
Contain 3mL breeding water；B group is solvent (0.1%DMSO, i.e. 0.1% dimethyl sulfoxide) control treatment group, as contains 3 μ L
The 3mL breeding water of DMSO；C group is that concentration is 200 μM of olibanum composite essential oil processing groups, i.e., 200 configured with 3mL breeding water
μ g/mL olibanum composite essential oil solution.
Each processing group selects 30 tails to develop consistent 6hpf zebra fish, be placed in 28 DEG C of incubators cultivate to
It takes pictures after 52hpf；After removing olibanum composite essential oil after taking pictures for 24 hours, take pictures again.
3, image analysis:
The zebra fish handled by olibanum composite essential oil to 52hpf is chosen into 10 tails immediately and is placed in stereoscopic microscope observing, bat
According to (back side sight) and save.Pass through the repressed situation of zebra fish melanin in the observation each processing group of comparative analysis qualitative evaluation
(Fig. 1 and Fig. 2)；
Zebra fish melanin inhibits to restore to test to show: the zebra handled by 46 hours 100 μ g/mL olibanum composite essential oils
Fish, discovery of being taken pictures, compared with the control group, melanin significantly reduces (Fig. 3 and Fig. 4), however removes by olibanum composite essential oil
After 24 hours, the melanin of zebra fish significantly increases, compared with being visually observed with control group, without significant difference, this
Illustrate that melanin after removing olibanum composite essential oil, is restored, this may be to inhibit because olibanum composite essential oil has
Melan- cell melanin generate and inhibit tyrosinase activity or promote melanocyte transport, transhipment and metabolism effect, institute
With olibanum composite essential oil to preventing and improve pigementation with good effect.
Embodiment 7:
The acute toxicity test of olibanum composite essential oil:
1, zebra fish is chosen:
The AB system zebra fish of 6hpf is placed under disecting microscope and is observed, picking is developed normal and consistent zebra fish and moved into
In 6 hole microwell plates, every 30 tail of hole.
2, compound is handled:
This test is tested using hydrostatic formula, and 6 different olibanum composite essential oil (concentration are respectively set according to preliminary result
For 0 μ g/mL, 10 μ g/mL, 100 μ g/mL, 1mg/mL, 2mg/mL, 3mg/mL) and arbutin concentration (concentration is 0 μM, 10 μM,
100 μM, 1mM, 2mM, 3mM), each concentration is put into 30 tails and develops consistent 6hpf zebra fish, is put into 28 DEG C of incubators and is protected from light
Culture, using the death rate of zebra fish as evaluation index.
3, test result:
By logging and statistical analysis, find:
(1) zebra fish by drug-treated to 52hpf when, it has been observed that arbutin and olibanum composite essential oil processing group, zebra
The maximum Sublethal concentration of fish is 3mM.
(2) zebra fish by drug-treated to 96hpf when, it has been observed that the maximum of arbutin processing group zebra fish is non-lethal
Concentration is about 1mM, and olibanum composite essential oil processing group is then 2mM.
In example 2, it is respectively 100 μM and 60 μ that arbutin and olibanum composite essential oil, which inhibit the action concentration of melanin,
G/mL, and maximum non-lethal dose of the two in acute toxicity testing concentration range is respectively 1mM and 2mM, that is, black bearberry
The maximum Sublethal concentration of glycosides and olibanum composite essential oil is 33 times times and 10 times of corresponding action concentration, result above table respectively
Bright, for olibanum composite essential oil compared with arbutin, acute toxicity is lower, therefore safety is more preferable.It is tested in application zebra fish model
It was found that olibanum composite essential oil to melanin can play it is effective inhibit or (and) decomposition, so that melanin spot is thoroughly disappeared,
The effect of whitening and nti-freckle can be played；Simultaneously by comparing with the arbutin of same concentrations, olibanum composite essential oil has melanin
There is stronger inhibitory effect, and just display is known as stronger inhibiting effect to black at low concentrations；And it is acute through zebra fish
Toxicity test shows that olibanum composite essential oil Acute Toxicity is low, highly effective and safe, therefore olibanum composite essential oil can be used to prepare
Drug with whitening and cosmetics or skin-whitening and treatment pigmentation disease.
Cosmetic formulations embodiment 1:
Above-mentioned olibanum composite essential oil and excipient weight of the invention is first made than for 3:1's by the method for embodiment 1-4
Excipient is added in ratio, and Eradicates spot Essence is made.
Cosmetic formulations embodiment 2:
Above-mentioned olibanum composite essential oil and excipient weight of the invention is first made than for 5:1's by the method for embodiment 1-4
Excipient is added in ratio, and Eradicates spot face cream or Eradicates spot late frost is made.
Cosmetic formulations embodiment 3:
Above-mentioned olibanum composite essential oil and excipient weight of the invention is first made than for 9:1's by the method for embodiment 1-4
Excipient is added in ratio, and Eradicates spot lotion is made.
Cosmetic formulations embodiment 4:
Above-mentioned olibanum composite essential oil and excipient weight of the invention is first made than for 9:1's by the method for embodiment 1-4
Excipient is added in ratio, and Eradicates spot face mask cream or Eradicates spot facemask powder is made.
Cosmetic formulations embodiment 5:
Above-mentioned olibanum composite essential oil and excipient weight of the invention is first made than for 9:1's by the method for embodiment 1-4
Excipient is added in ratio, and Eradicates spot spray is made.
Pharmaceutical preparation embodiment 1:
Above-mentioned olibanum composite essential oil and excipient weight of the invention is first made than for 9:1's by the method for embodiment 1-4
Excipient is added in ratio, and pulvis is made.
Pharmaceutical preparation embodiment 2:
It is 1 that above-mentioned olibanum composite essential oil of the invention, which is first made, by itself and excipient weight ratio by the method for embodiment 1-4:
Excipient, pelletizing press sheet is added in the ratio of 5-1:10.
Pharmaceutical preparation embodiment 3:
Above-mentioned olibanum composite essential oil of the invention is first made by the method for embodiment 1-4, and routinely mouth is made in oral solution preparation method
Take liquid.
Pharmaceutical preparation embodiment 4:
Above-mentioned olibanum composite essential oil of the invention is first made by the method for embodiment 1-4, capsule or granule or punching is made
Agent.
Pharmaceutical preparation embodiment 5:
It is 3 that above-mentioned olibanum composite essential oil of the invention, which is first made, by itself and excipient weight ratio by the method for embodiment 1-4:
Excipient is added in 1 ratio, and capsule or granule or electuary is made.
Claims (10)
1. a kind of olibanum composite essential oil, which is characterized in that by mass percentage, including olibanum essential oil 1%-5%, Rosa Damascana
8-15%, Rosemary Oil 3-8%, evening primrose oil 8-10%, base oil 62-80%.
2. olibanum composite essential oil according to claim 1, which is characterized in that the base oil by Sweet Almond Oil, SIMMONDSIA CHINENSIS SEED OIL,
One or more of grape seed oil, prinsepia utilis royle oil, olive oil, rose hip oil are by any proportion mixing composition.
3. olibanum composite essential oil according to claim 1, which is characterized in that the olibanum includes Burseraceae Boswellia
The resin of bark exudation.
4. olibanum composite essential oil according to claim 1, which is characterized in that the rosemary includes that Labiatae Rosmarinus is planted
Object.
5. olibanum composite essential oil according to claim 1, which is characterized in that the rose includes rosaceae rose.
6. olibanum composite essential oil according to claim 1, which is characterized in that the oenothera biennis includes Oenotheraceae Oenothera
Plant.
7. olibanum composite essential oil according to claim 1, which is characterized in that the olibanum essential oil extracts by the following method
It arrives:
(1) it ferments: choosing Boswellia carterii resin, pile fermentation is placed about 1-2 days, and the coarse granule for being then crushed to 30-60 mesh is spare.
(2) it extracts: being extracted in 5.0-6.0 hydrochloric acid or aqueous sulfuric acid by the pH value that coarse granule is soaked in 7-9 times of quality
Night obtains extraction mixed liquor.
(3) it digests: being added cellulase and pectase into extraction mixed liquor, cellulase, pectase and extraction mixed liquor
Quality proportioning is 0.5-1:0.1-0.5:100, is digested about 2-3 hours at 35-37 DEG C.
(4) it extracts: the mixed liquor after step 3 enzymatic hydrolysis being put into steam distillation and is extracted in instrument, 100 DEG C of -120 DEG C of refluxing extractions
About 5-7 hour collects distillate, is cooled to room temperature to be placed in oil water separator and separates, oil reservoir is dry with anhydrous sodium sulfate,
Separation is to get olibanum essential oil；Or the mixed liquor after step 3 enzymatic hydrolysis is put into CO 2 supercritical and is extracted in instrument, extraction
- 48 DEG C of temperature 45 C, the flow velocity of pressure 13.70MPa-17.80MPa, time 50min-100min, carbon dioxide is
25kg/h-35kg/h；Extract is collected, is placed in molecular distillation instrument and refines, feeding temperature is 40 DEG C -45 DEG C, sample introduction speed
For 480-530mL/h, one section of evaporating temperature is 110 DEG C -115 DEG C, and dual circulation temperature is 60 DEG C -65 DEG C, collects two sections of distillations
Part obtains olibanum essential oil.
8. olibanum composite essential oil according to claim 1, which is characterized in that the extraction of the Rosa Damascana, Rosemary Oil
Method is identical, comprising the following steps: raw material is put into steam distillation and is extracted in instrument, the saturated common salt of 7-9 times of quality is added
Water, 3-5 hour of 100 DEG C of -120 DEG C of refluxing extractions collect distillate, are cooled to room temperature to be placed in oil water separator and separate,
Oil reservoir is dry with anhydrous sodium sulfate, separates to get essential oil.The raw material is fresh rose or rosemary；The essential oil is rose
Essential oil or Rosemary Oil.
9. olibanum composite essential oil according to claim 1, which is characterized in that the evening primrose oil is extracted by the following method
To: after oenothera seed is crushed to 60-80 mesh, the lime white that the concentration that 4-8 times of quality is added is 1-2% adjusts pH value to 11-
13, it after impregnating 6-8 hours, is put into squeezer and squeezes, after collecting juicing filtering, be placed in supercentrifuge, 5000-7000
Rev/min by water-oil separating, oil reservoir is placed in molecular distillation instrument and refines, and feeding temperature is 40 DEG C -45 DEG C, and sample introduction speed is
480-530mL/h, one section of evaporating temperature are 110 DEG C -115 DEG C, and dual circulation temperature is 60 DEG C -65 DEG C, collect one section of distiller
Point, obtain evening primrose oil.
10. the application of olibanum composite essential oil described in a kind of claim 1, which is characterized in that the application includes olibanum composite precision
Oil preparation treatment pigmentation disease drug in application, olibanum composite essential oil preparing skin-whitening and nti-freckle health
Application in product.The health-oriented products include massage oil, cosmetics, showering agent and fragrance fumette.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110772456A (en) * | 2019-11-04 | 2020-02-11 | 中国热带农业科学院南亚热带作物研究所 | Miracle fruit leaf compound and application thereof in anti-melanin formation product |
| CN111956530A (en) * | 2020-08-31 | 2020-11-20 | 广州丽彦妆生物科技有限公司 | Production process of rose oil |
| CN115531473A (en) * | 2022-11-23 | 2022-12-30 | 梵申兰科技(上海)有限公司 | Compound essential oil for treating skin diseases and pharmaceutical composition containing same |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106860059A (en) * | 2015-12-13 | 2017-06-20 | 闫战军 | A kind of rose root of Dahurain angelica essential oil beauty face-whitening-nourishing essential oils and preparation method thereof |
| CN108201516A (en) * | 2016-12-16 | 2018-06-26 | 闫战军 | A kind of rose maintenance udder massage essential oil and preparation method thereof |
| CN108201518A (en) * | 2016-12-18 | 2018-06-26 | 闫战军 | A kind of rosemary fennel beautifying wrinkle-removing essential oil and preparation method thereof |
| CN108309832A (en) * | 2018-04-03 | 2018-07-24 | 余道逍 | A kind of whitening compound essential oil |
| CN109355137A (en) * | 2018-11-21 | 2019-02-19 | 黛君本草生命科技（深圳）有限公司 | A kind of preparation process of cinnamon essential oil |
-
2019
- 2019-05-30 CN CN201910461835.XA patent/CN110201043A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106860059A (en) * | 2015-12-13 | 2017-06-20 | 闫战军 | A kind of rose root of Dahurain angelica essential oil beauty face-whitening-nourishing essential oils and preparation method thereof |
| CN108201516A (en) * | 2016-12-16 | 2018-06-26 | 闫战军 | A kind of rose maintenance udder massage essential oil and preparation method thereof |
| CN108201518A (en) * | 2016-12-18 | 2018-06-26 | 闫战军 | A kind of rosemary fennel beautifying wrinkle-removing essential oil and preparation method thereof |
| CN108309832A (en) * | 2018-04-03 | 2018-07-24 | 余道逍 | A kind of whitening compound essential oil |
| CN109355137A (en) * | 2018-11-21 | 2019-02-19 | 黛君本草生命科技（深圳）有限公司 | A kind of preparation process of cinnamon essential oil |
Non-Patent Citations (1)
| Title |
|---|
| 《轻图典》编辑部著: "《疗效精油轻图典》", 30 September 2012, 江西科学技术出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110772456A (en) * | 2019-11-04 | 2020-02-11 | 中国热带农业科学院南亚热带作物研究所 | Miracle fruit leaf compound and application thereof in anti-melanin formation product |
| CN110772456B (en) * | 2019-11-04 | 2022-08-23 | 中国热带农业科学院南亚热带作物研究所 | Miracle fruit leaf compound and application thereof in anti-melanin formation product |
| CN111956530A (en) * | 2020-08-31 | 2020-11-20 | 广州丽彦妆生物科技有限公司 | Production process of rose oil |
| CN115531473A (en) * | 2022-11-23 | 2022-12-30 | 梵申兰科技(上海)有限公司 | Compound essential oil for treating skin diseases and pharmaceutical composition containing same |
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