CN106632585A - Process for preparing crude protein by using cottonseed meal as raw material - Google Patents
Process for preparing crude protein by using cottonseed meal as raw material Download PDFInfo
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- CN106632585A CN106632585A CN201710093786.XA CN201710093786A CN106632585A CN 106632585 A CN106632585 A CN 106632585A CN 201710093786 A CN201710093786 A CN 201710093786A CN 106632585 A CN106632585 A CN 106632585A
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- cottonseed meal
- crude protein
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- cottonseed
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
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- Proteomics, Peptides & Aminoacids (AREA)
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Abstract
The invention discloses a process for preparing crude protein by using cottonseed meal as a raw material. The process includes: (1) immersing the cottonseed meal to degrease, and filtering to obtain degreased cottonseed meal; (2) drying the degreased cottonseed meal, and smashing to obtain smashed cottonseed meal; (3) mixing the smashed cottonseed meal with an aqueous sodium hydroxide solution, and lixiviating to obtain cottonseed leach liquor; (4) subjecting the cottonseed leach liquor to centrifugal separation, taking upper supernate as a standby; (5) adjusting pH (potential of hydrogen) of the upper supernate by an acid and alkali agent, standing still, subjecting the upper supernate to centrifugal separation, and taking lower precipitate as the crude protein. Compared with the prior art, the process for preparing the crude protein by using the cottonseed meal as the raw material has the advantages that simplicity in process, short extraction period and low preparing cost are achieved, the high-quality crude protein can be extracted from the cottonseed meal quickly and efficiently by low energy consumption, an extraction rate reaches to above 45%, purity of the prepared crude protein reaches to above 70%, a promising market prospect is achieved, and economic benefits are obtained, so that the process is worth being popularized.
Description
Technical field
The invention belongs to agricultural byproducts processing technical field, and in particular to a kind of extraction as raw material with cottonseed meal prepares thick egg
White technique.
Background technology
Processing of farm products garbage mainly including the peel residue of drink manufacturing industry, form sediment by the leftover bits and pieces of poultry processing, production
The manioc waste of powder, potato residues, the megasse of sugar industry, bagasse, rice husk, Testa Tritici, dregs of rapeseed cake, the bean dregs after grain and oil processing
Deng milk surum and changing food waste in distiller grains, the diary product process of brewing industry etc..These garbages if do not made proper arrangements,
The very big pollution of environment will certainly be caused.It is high with respect to Development volue and these garbages still contain substantial amounts of organic matter, therefore
These processing of farm products garbages are produced into protein product by various recycling technological means, agriculture not only can be extended
The industrial chain of Product processing, obtains preferable economic benefit, and can solve problem of environmental pollution.
Semen Gossypii is extracted in the cottonseed meal after oils and fatss containing substantial amounts of protein, and its content is about 50% or so, is a kind of
Extremely abundant vegetable protein resource.The property of cottonseed protein is similar with soybean protein, be also one kind be of high nutritive value, product
Good protein food of fine quality or feed protein source.About with cottonseed meal as the existing related text of the technology that raw material extraction prepares albumen
Report is offered, such as：Chinese patent（Application publication number：102550794A）Disclose a kind of side that cottonseed protein is extracted from cottonseed meal
Method, including：(1) cottonseed meal is crushed；(2) ultrasonic Treatment cottonseed meal；(3) bollworm organized enzyme is extracted；(4) cottonseed meal enzyme
Solution；(5) intensification enzyme denaturing, is then centrifuged for separating, and obtains supernatant and filtering residue；(6) filtering residue is added water and is washed, water lotion and step
(5) supernatant obtained in merges；(7) supernatant after merging is obtained Jing after microfiltration membrane impurity removal, nanofiltration membrance concentration, drying
Powdery cottonseed protein.The inventive method causes the production of cottonseed protein to have higher extraction ratio, and product with stronger
Functional activity.But technique is complex, cost of manufacture is high.
The content of the invention
The purpose of the present invention has aimed to provide a kind of technique for preparing crude protein as raw material extraction with cottonseed meal.
To realize the object of the invention, technical scheme below is employed：One kind prepares crude protein by raw material extraction of cottonseed meal
Technique, comprise the following steps：
（1）Take the immersion of cottonseed meal ethanol and slough oils and fatss, then filter, obtain degreased cottonseed meal；
（2）Degreased cottonseed meal is dried, is crushed, obtain cottonseed meal crushed material；
（3）Cottonseed meal crushed material is mixed with sodium hydrate aqueous solution, under the conditions of temperature is for 40-50 DEG C 50-100min is extracted,
Obtain cottonseed meal lixiviating solution；
（4）By cottonseed meal lixiviating solution centrifugation, upper strata supernatant is taken, it is standby；
（5）It is 4.5-4.7 by supernatant soda acid agent regulation pH, stands 1-3h, be then centrifuged for separating supernatant, removes layer and sink
Form sediment, obtain final product the crude protein.
In step（1）In, the concentration of the ethanol is 90-95%, and soak time is 1-2h；The cottonseed meal and ethanol
Amount ratio is 1g：6-12ml.
In step（2）In, the degreased cottonseed meal is dried to constant weight under the conditions of 50-80 DEG C, the cottonseed meal crushed material
Particle diameter be 120-250 μm.
In step（3）In, the sodium hydrate aqueous solution concentration be 0.01-0.03mol/L, the cottonseed meal crushed material with
The mass ratio of sodium hydrate aqueous solution is 1:6-12.
In step（4）In, the centrifugation is that 8-10min is centrifuged under the conditions of 3000-3500r/min.
In step（5）In, the sulphuric acid of the soda acid agent 0.5mol/L, the centrifugation is in 3000-3500r/min bars
8-10min is centrifuged under part.
Compared with prior art, present invention process method and step is simple, and extracting cycle is short, and preparation cost is low, can quickly,
Efficiently, the crude protein of high-quality is extracted to energy-conservation from cottonseed meal, extraction rate reached more than 45%, the prepared crude protein for obtaining is pure
Degree, with good market prospect and economic benefit, is worthy of popularization up to more than 70%.
Specific embodiment
The present invention is described in further detail with reference to embodiments, the present embodiment is only to make clearer to the present invention
Illustrate, rather than limitation of the present invention.
Embodiment 1
A kind of technique for preparing crude protein as raw material extraction with cottonseed meal, comprises the following steps：
（1）It is 1g by amount ratio to take 90% ethanol of cottonseed meal：9ml immersion 3h slough oils and fatss, then filter, and obtain degreased cottonseed meal；
（2）Degreased cottonseed meal is dried to constant weight under the conditions of 50 DEG C, is crushed, cross 90 mesh sieves, obtain cottonseed meal crushed material；
（3）In mass ratio it is 1 by cottonseed meal crushed material and 0.03mol/L sodium hydrate aqueous solutions:6 mixing, in temperature be 45 DEG C
Under the conditions of extract 100min, obtain cottonseed meal lixiviating solution；
（4）Cottonseed meal lixiviating solution is centrifuged into 9min under the conditions of 3000r/min, upper strata supernatant is taken, it is standby；
（5）With the sulfur acid for adjusting pH of 0.5mol/L it is 4.6 by supernatant, 1h is stood, then by supernatant in 3300r/min conditions
Lower centrifugation 10min, takes lower sediment, obtains final product the crude protein.
Jing is determined, and the present embodiment technique extraction ratio is 46%, and the prepared crude protein purity for obtaining is 72%.
Embodiment 2
A kind of technique for preparing crude protein as raw material extraction with cottonseed meal, comprises the following steps：
（1）It is 1g by amount ratio to take 92% ethanol of cottonseed meal：12ml immersion 1h slough oils and fatss, then filter, and obtain degreased cottonseed
The dregs of rice；
（2）Degreased cottonseed meal is dried to constant weight under the conditions of 65 DEG C, is crushed, cross 120 mesh sieves, obtain cottonseed meal crushed material；
（3）In mass ratio it is 1 by cottonseed meal crushed material and 0.01mol/L sodium hydrate aqueous solutions:9 mixing, in temperature be 50 DEG C
Under the conditions of extract 50min, obtain cottonseed meal lixiviating solution；
（4）Cottonseed meal lixiviating solution is centrifuged into 10min under the conditions of 3200r/min, upper strata supernatant is taken, it is standby；
（5）With the sulfur acid for adjusting pH of 0.5mol/L it is 4.5 by supernatant, 2h is stood, then by supernatant in 3500r/min conditions
Lower centrifugation 8min, takes lower sediment, obtains final product the crude protein.
Jing is determined, and the present embodiment technique extraction ratio is 47%, and the prepared crude protein purity for obtaining is 73%.
Embodiment 3
A kind of technique for preparing crude protein as raw material extraction with cottonseed meal, comprises the following steps：
（1）It is 1g by amount ratio to take 95% ethanol of cottonseed meal：6ml immersion 1.5h slough oils and fatss, then filter, and obtain degreased cottonseed
The dregs of rice；
（2）Degreased cottonseed meal is dried to constant weight under the conditions of 80 DEG C, is crushed, cross 60 mesh sieves, obtain cottonseed meal crushed material；
（3）In mass ratio it is 1 by cottonseed meal crushed material and 0.02mol/L sodium hydrate aqueous solutions:12 mixing, in temperature be 40 DEG C
Under the conditions of extract 75min, obtain cottonseed meal lixiviating solution；
（4）Cottonseed meal lixiviating solution is centrifuged into 8min under the conditions of 3500r/min, upper strata supernatant is taken, it is standby；
（5）With the sulfur acid for adjusting pH of 0.5mol/L it is 4.6 by supernatant, 3h is stood, then by supernatant in 3000r/min conditions
Lower centrifugation 9min, takes lower sediment, obtains final product the crude protein.
Jing is determined, and the present embodiment technique extraction ratio is 45%, and the prepared crude protein purity for obtaining is 74%.
The basic principles, principal features and advantages of the present invention have been shown and described above.Those skilled in the art should
Understand, the present invention is not restricted to the described embodiments, the original for simply illustrating the present invention described in above-described embodiment and description
Reason, without departing from the spirit and scope of the present invention, the present invention also has various changes and modifications, these changes and improvements
Both fall within scope of the claimed invention.The claimed scope of the invention is by appending claims and its equivalent circle.
It is fixed.
Claims (7)
1. a kind of technique that crude protein is prepared as raw material extraction with cottonseed meal, it is characterised in that comprise the following steps：
（1）Take the immersion of cottonseed meal ethanol and slough oils and fatss, then filter, obtain degreased cottonseed meal；
（2）Degreased cottonseed meal is dried, is crushed, obtain cottonseed meal crushed material；
（3）Cottonseed meal crushed material is mixed with sodium hydrate aqueous solution, under the conditions of temperature is for 40-50 DEG C 50-100min is extracted,
Obtain cottonseed meal lixiviating solution；
（4）By cottonseed meal lixiviating solution centrifugation, upper strata supernatant is taken, it is standby；
（5）It is 4.5-4.7 by supernatant soda acid agent regulation pH, stands 1-3h, be then centrifuged for separating supernatant, removes layer and sink
Form sediment, obtain final product the crude protein.
2. the technique for as claimed in claim 1 crude protein being prepared as raw material extraction with cottonseed meal, it is characterised in that step（1）In,
The concentration of the ethanol is 90-95%, and soak time is 1-2h.
3. the technique for as claimed in claim 2 crude protein being prepared as raw material extraction with cottonseed meal, it is characterised in that the cottonseed meal
It is 1g with the amount ratio of ethanol：6-12ml.
4. the technique for as claimed in claim 1 crude protein being prepared as raw material extraction with cottonseed meal, it is characterised in that step（2）In,
The degreased cottonseed meal is dried to constant weight under the conditions of 50-80 DEG C, and the particle diameter of the cottonseed meal crushed material is 120-250 μm.
5. the technique for as claimed in claim 1 crude protein being prepared as raw material extraction with cottonseed meal, it is characterised in that step（3）In,
The sodium hydrate aqueous solution concentration is the quality of 0.01-0.03mol/L, the cottonseed meal crushed material and sodium hydrate aqueous solution
Than for 1:6-12.
6. the technique for as claimed in claim 1 crude protein being prepared as raw material extraction with cottonseed meal, it is characterised in that step（4）In,
The centrifugation is that 8-10min is centrifuged under the conditions of 3000-3500r/min.
7. the technique for as claimed in claim 1 crude protein being prepared as raw material extraction with cottonseed meal, it is characterised in that step（5）In,
The sulphuric acid of the soda acid agent 0.5mol/L, the centrifugation is that 8-10min is centrifuged under the conditions of 3000-3500r/min.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710093786.XA CN106632585A (en) | 2017-02-21 | 2017-02-21 | Process for preparing crude protein by using cottonseed meal as raw material |
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| CN201710093786.XA CN106632585A (en) | 2017-02-21 | 2017-02-21 | Process for preparing crude protein by using cottonseed meal as raw material |
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| CN106632585A true CN106632585A (en) | 2017-05-10 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110606866A (en) * | 2019-10-25 | 2019-12-24 | 仲恺农业工程学院 | Method for refining cottonseed protein |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20040059105A (en) * | 2002-12-27 | 2004-07-05 | 이옥구 | A process of producing water soluble and water insoluble hemicellulose |
| CN105010719A (en) * | 2015-08-12 | 2015-11-04 | 重庆都好生物科技有限公司 | Extraction method of pumpkin seed meal protein |
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2017
- 2017-02-21 CN CN201710093786.XA patent/CN106632585A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20040059105A (en) * | 2002-12-27 | 2004-07-05 | 이옥구 | A process of producing water soluble and water insoluble hemicellulose |
| CN105010719A (en) * | 2015-08-12 | 2015-11-04 | 重庆都好生物科技有限公司 | Extraction method of pumpkin seed meal protein |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110606866A (en) * | 2019-10-25 | 2019-12-24 | 仲恺农业工程学院 | Method for refining cottonseed protein |
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Application publication date: 20170510 |