CN106619730B - Medicine for treating hepatitis A and preparation method thereof - Google Patents
Medicine for treating hepatitis A and preparation method thereof Download PDFInfo
- Publication number
- CN106619730B CN106619730B CN201611258340.XA CN201611258340A CN106619730B CN 106619730 B CN106619730 B CN 106619730B CN 201611258340 A CN201611258340 A CN 201611258340A CN 106619730 B CN106619730 B CN 106619730B
- Authority
- CN
- China
- Prior art keywords
- ethanol
- periplaneta americana
- hepatitis
- extract
- medicine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000003814 drug Substances 0.000 title claims abstract description 90
- 208000005252 hepatitis A Diseases 0.000 title claims abstract description 85
- 238000002360 preparation method Methods 0.000 title abstract description 33
- 229940079593 drug Drugs 0.000 title description 9
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 180
- 241000238675 Periplaneta americana Species 0.000 claims abstract description 114
- 239000000284 extract Substances 0.000 claims abstract description 53
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 49
- 238000002137 ultrasound extraction Methods 0.000 claims abstract description 39
- 238000003756 stirring Methods 0.000 claims abstract description 32
- 238000002791 soaking Methods 0.000 claims abstract description 22
- 238000000034 method Methods 0.000 claims abstract description 21
- 244000303040 Glycyrrhiza glabra Species 0.000 claims abstract description 13
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 claims abstract description 13
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 claims abstract description 13
- 235000011477 liquorice Nutrition 0.000 claims abstract description 13
- 235000009051 Ambrosia paniculata var. peruviana Nutrition 0.000 claims abstract description 12
- 235000003097 Artemisia absinthium Nutrition 0.000 claims abstract description 12
- 240000001851 Artemisia dracunculus Species 0.000 claims abstract description 12
- 235000017731 Artemisia dracunculus ssp. dracunculus Nutrition 0.000 claims abstract description 12
- 235000003261 Artemisia vulgaris Nutrition 0.000 claims abstract description 12
- 244000153955 Reynoutria sachalinensis Species 0.000 claims abstract description 12
- 235000003202 Reynoutria sachalinensis Nutrition 0.000 claims abstract description 12
- 239000001138 artemisia absinthium Substances 0.000 claims abstract description 12
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000000843 powder Substances 0.000 claims description 76
- 239000000243 solution Substances 0.000 claims description 43
- 239000007788 liquid Substances 0.000 claims description 36
- 238000001914 filtration Methods 0.000 claims description 25
- 238000002156 mixing Methods 0.000 claims description 23
- 239000011259 mixed solution Substances 0.000 claims description 22
- 230000005484 gravity Effects 0.000 claims description 20
- 238000000605 extraction Methods 0.000 claims description 17
- 239000000469 ethanolic extract Substances 0.000 claims description 10
- 238000010438 heat treatment Methods 0.000 claims description 10
- 239000008213 purified water Substances 0.000 claims description 10
- 238000007599 discharging Methods 0.000 claims description 9
- 239000000706 filtrate Substances 0.000 claims description 9
- 238000007873 sieving Methods 0.000 claims description 9
- 239000002994 raw material Substances 0.000 claims description 3
- 238000002481 ethanol extraction Methods 0.000 claims description 2
- 230000008569 process Effects 0.000 claims description 2
- 238000003809 water extraction Methods 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 11
- 150000001413 amino acids Chemical class 0.000 abstract description 9
- 239000000463 material Substances 0.000 abstract description 9
- 210000005229 liver cell Anatomy 0.000 abstract description 6
- CZPWVGJYEJSRLH-UHFFFAOYSA-N Pyrimidine Chemical compound C1=CN=CN=C1 CZPWVGJYEJSRLH-UHFFFAOYSA-N 0.000 abstract description 3
- 239000000203 mixture Substances 0.000 abstract description 2
- 230000001338 necrotic effect Effects 0.000 abstract description 2
- 230000008929 regeneration Effects 0.000 abstract description 2
- 238000011069 regeneration method Methods 0.000 abstract description 2
- 208000024891 symptom Diseases 0.000 description 37
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 24
- 206010061428 decreased appetite Diseases 0.000 description 22
- 206010028813 Nausea Diseases 0.000 description 21
- 208000022531 anorexia Diseases 0.000 description 21
- 230000008693 nausea Effects 0.000 description 21
- 210000004185 liver Anatomy 0.000 description 18
- 201000010099 disease Diseases 0.000 description 15
- 206010047700 Vomiting Diseases 0.000 description 13
- JUJWROOIHBZHMG-UHFFFAOYSA-N Pyridine Chemical compound C1=CC=NC=C1 JUJWROOIHBZHMG-UHFFFAOYSA-N 0.000 description 12
- 208000006083 Hypokinesia Diseases 0.000 description 11
- 206010063659 Aversion Diseases 0.000 description 10
- 206010012735 Diarrhoea Diseases 0.000 description 9
- 238000010298 pulverizing process Methods 0.000 description 8
- 230000008673 vomiting Effects 0.000 description 8
- 206010000060 Abdominal distension Diseases 0.000 description 7
- 206010023126 Jaundice Diseases 0.000 description 7
- 208000002193 Pain Diseases 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 7
- 238000007789 sealing Methods 0.000 description 7
- UMJSCPRVCHMLSP-UHFFFAOYSA-N pyridine Natural products COC1=CC=CN=C1 UMJSCPRVCHMLSP-UHFFFAOYSA-N 0.000 description 6
- 210000003786 sclera Anatomy 0.000 description 6
- 206010030113 Oedema Diseases 0.000 description 5
- 238000009835 boiling Methods 0.000 description 5
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 5
- 241000238661 Periplaneta Species 0.000 description 4
- 206010042674 Swelling Diseases 0.000 description 4
- 208000019790 abdominal distention Diseases 0.000 description 4
- 238000003745 diagnosis Methods 0.000 description 4
- 230000008961 swelling Effects 0.000 description 4
- 210000002700 urine Anatomy 0.000 description 4
- 241000709721 Hepatovirus A Species 0.000 description 3
- 206010037660 Pyrexia Diseases 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 201000009240 nasopharyngitis Diseases 0.000 description 3
- 210000003800 pharynx Anatomy 0.000 description 3
- 210000000952 spleen Anatomy 0.000 description 3
- 210000002784 stomach Anatomy 0.000 description 3
- 241000205585 Aquilegia canadensis Species 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical group OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 206010010774 Constipation Diseases 0.000 description 2
- 206010019705 Hepatic pain Diseases 0.000 description 2
- 241000238631 Hexapoda Species 0.000 description 2
- 241000827125 Lupinus flavoculatus Species 0.000 description 2
- 206010048245 Yellow skin Diseases 0.000 description 2
- 210000000941 bile Anatomy 0.000 description 2
- 235000019658 bitter taste Nutrition 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 230000006866 deterioration Effects 0.000 description 2
- 230000004064 dysfunction Effects 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 210000000232 gallbladder Anatomy 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 210000000936 intestine Anatomy 0.000 description 2
- 238000001990 intravenous administration Methods 0.000 description 2
- 230000003908 liver function Effects 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 206010003399 Arthropod bite Diseases 0.000 description 1
- 241000238659 Blatta Species 0.000 description 1
- 206010007247 Carbuncle Diseases 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Chemical group OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- 206010053155 Epigastric discomfort Diseases 0.000 description 1
- 206010017553 Furuncle Diseases 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 208000015817 Infant Nutrition disease Diseases 0.000 description 1
- 241000500891 Insecta Species 0.000 description 1
- 241000334160 Isatis Species 0.000 description 1
- 244000179560 Prunella vulgaris Species 0.000 description 1
- 239000008923 Qingkailing Substances 0.000 description 1
- 235000018167 Reynoutria japonica Nutrition 0.000 description 1
- 240000001341 Reynoutria japonica Species 0.000 description 1
- 208000004078 Snake Bites Diseases 0.000 description 1
- 240000001949 Taraxacum officinale Species 0.000 description 1
- 235000005187 Taraxacum officinale ssp. officinale Nutrition 0.000 description 1
- 206010058874 Viraemia Diseases 0.000 description 1
- 229930003270 Vitamin B Chemical group 0.000 description 1
- 229930003268 Vitamin C Chemical group 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000003443 antiviral agent Substances 0.000 description 1
- 208000027697 autoimmune lymphoproliferative syndrome due to CTLA4 haploinsuffiency Diseases 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical group OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000008602 contraction Effects 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 235000001727 glucose Nutrition 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 230000003907 kidney function Effects 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 230000005976 liver dysfunction Effects 0.000 description 1
- TTWJBBZEZQICBI-UHFFFAOYSA-N metoclopramide Chemical compound CCN(CC)CCNC(=O)C1=CC(Cl)=C(N)C=C1OC TTWJBBZEZQICBI-UHFFFAOYSA-N 0.000 description 1
- 229960004503 metoclopramide Drugs 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000002741 palatine tonsil Anatomy 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 206010036067 polydipsia Diseases 0.000 description 1
- 150000003839 salts Chemical group 0.000 description 1
- 230000036186 satiety Effects 0.000 description 1
- 235000019627 satiety Nutrition 0.000 description 1
- 235000008113 selfheal Nutrition 0.000 description 1
- 235000002639 sodium chloride Nutrition 0.000 description 1
- 208000010731 swelling symptom Diseases 0.000 description 1
- 208000006379 syphilis Diseases 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 235000019640 taste Nutrition 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 206010044008 tonsillitis Diseases 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 201000002327 urinary tract obstruction Diseases 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 231100000611 venom Toxicity 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Chemical group 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Chemical group 0.000 description 1
- 210000004916 vomit Anatomy 0.000 description 1
- 238000004383 yellowing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/484—Glycyrrhiza (licorice)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/70—Polygonaceae (Buckwheat family), e.g. spineflower or dock
- A61K36/704—Polygonum, e.g. knotweed
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Epidemiology (AREA)
- Alternative & Traditional Medicine (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Botany (AREA)
- Biotechnology (AREA)
- Engineering & Computer Science (AREA)
- Insects & Arthropods (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a medicine for treating hepatitis A and a preparation method thereof. The method is realized by adopting the following steps: soaking periplaneta americana with 60-95% ethanol, performing ultrasonic extraction at 30-50 ℃ for 3-5 times, concentrating the obtained extracting solution under reduced pressure to obtain an ethanol concentrated solution of the periplaneta americana, adding water into the concentrated solution, stirring, standing at a constant temperature for layering, and concentrating the obtained lower-layer water solution under reduced pressure to obtain the periplaneta americana extract for treating hepatitis A, wherein the insoluble matter content in the obtained extract is lower than 5%, the total amino acid content is higher than 15.0%, and the pyrimidine base content is higher than 0.1%. The obtained extract is compatible with traditional Chinese medicinal materials, has unique curative effect on treating icteric hepatitis A, and has the following mixture ratio: 15-25 parts of oriental wormwood, 15-25 parts of giant knotweed, 5-7 parts of liquorice and 0.15-0.25 part of periplaneta americana extract. When the periplaneta americana extract is used for treating hepatitis A, the regeneration of liver cell tissues can be promoted, the falling of necrotic liver cells is accelerated, damaged liver cells are quickly repaired, and the hepatitis A is cured.
Description
Technical Field
The invention relates to a preparation method of a medicine, in particular to a medicine for treating hepatitis A and a preparation method thereof.
Background
Hepatitis A virus is of the hepadnavirus genus. HAV enters the body orally and enters the bloodstream through the intestine, causing viremia, reaching the liver about one week later, and then draining into the intestine through the bile and the appearance of feces. The detoxification of the feces can be maintained for 1-2 weeks. The major organ of viral invasion is the liver, and the pharynx and tonsils may be sites of extrahepatic HAV reproduction.
After hepatitis A virus infection, patients can have symptoms of fever, hypodynamia, anorexia, oil aversion, yellow sclera infection, liver pain, liver swelling and abnormal rise of liver function indexes, and a few patients can also have symptoms of edema, encephaledema and liver function deterioration caused by renal function deterioration. Icteric hepatitis A is characterized by yellow eyes, yellow body and yellow urine caused by damp-turbidity obstruction, dysfunction of spleen, stomach, liver and gallbladder, gallbladder fluid failing to circulate normally and overflowing with blood due to affection of exogenous pathogenic factors such as damp-heat and epidemic toxin. It can be used for all people. Hunger and satiety or excessive drinking can damage the spleen and stomach, leading to dysfunction of the spleen and stomach in transportation and transformation, liver dysfunction, bile flow failure, overflow with blood, yellow skin, urinary obstruction, yellow body, and so on.
The hepatitis A is a self-limiting disease, excessive drug treatment is not needed, antiviral drugs are not needed, and the existing treatment method mainly comprises rest and support treatment. Avoid drinking, fatigue and using drugs that damage the liver; the food is easy to digest and rich in nutrition, and fresh vegetables, fruits and the like; patients who can not eat food, such as intravenous transfusion, can supply enough glucose, salt, vitamin C and vitamin B group, etc., pay attention to water and electrolyte balance, and patients with nausea, emesis and anorexia can be treated by multienzyme tablet, metoclopramide, etc.; the Chinese medicinal preparation has obvious effect of treating hepatitis A, such as oral compound honeysuckle granules, intravenous drip compound herba artemisiae capillaris injection, qingkailing injection and the like. The effect of oral traditional Chinese medicine decoction is good, such as dandelion, selfheal, isatis root, honeysuckle and the like.
Periplaneta americana is an insect of the genus Periplaneta of the family Periplaneta of the order Periplaneta of the class Insecta, commonly called Blatta seu periplaneta, the largest volume insect of the family Blattadae, native to south America. Periplaneta americana is a traditional Chinese medicinal material, and is recorded in Shen nong's herbal Jing, which is called salty and cold in taste. Mainly has the effects of blood stasis, hard mass, cold and heat, mass breaking, throat and throat contraction, internal cold and no seed, and is used for treating infantile malnutrition, furuncle, carbuncle, tonsillitis, innominate toxic swelling, syphilis, venomous snake bite, centipede bite and the like.
Disclosure of Invention
The invention aims to provide a medicine extracted from periplaneta americana for treating hepatitis A and a preparation method thereof.
The invention adopts the following preparation method to realize the purpose:
A. sieving dried periplaneta americana bodies with a 10-40-mesh sieve, crushing, soaking the obtained periplaneta americana coarse powder in 60-95% ethanol until the liquid level of the ethanol is 5-10 cm higher than the liquid level of the coarse powder, and hermetically storing for more than one week and less than 18 months to prepare a coarse powder mixed solution;
B. and C, carrying out ultrasonic extraction on the mixed solution of the coarse powder obtained in the step A for 3-5 times at the temperature of 30-50 ℃, wherein the ultrasonic power is as follows: 20-30KHz, adding 60-95% ethanol 5-8 times the weight of the coarse powder when the coarse powder mixed solution is extracted for the first time, wherein the extraction time is 30-60 min; adding 60-95% ethanol in an amount which is 3-6 times the weight of the coarse powder for each extraction after the second extraction, wherein the extraction time is 2-50 min; mixing the extractive solutions, and filtering to obtain Periplaneta americana ethanol extractive solution;
C. concentrating the ethanol extract obtained in the step B under reduced pressure under the conditions of vacuum degree of-0.05 to-0.06 MPa and temperature of 50 to 60 ℃ until the specific gravity is 1.05 to 1.15 to obtain an American cockroach body ethanol concentrated solution;
D. adding purified water which is 7-10 times of the weight of the American cockroach body ethanol concentrated solution obtained in the step C into the American cockroach body ethanol concentrated solution, stirring and heating to 85-95 ℃, stirring at a constant temperature for 10-60 minutes at a stirring speed of 20-60 revolutions per minute, and then keeping the temperature and standing for 1-5 hours to enable the American cockroach body ethanol concentrated solution to be layered;
E. and D, discharging the lower-layer water liquid obtained in the step D, filtering the lower-layer water liquid by using filter paper, and concentrating the filtrate under reduced pressure at the temperature of 60-80 ℃ and the vacuum degree of-0.05-0.06 MPa until the specific gravity is 1.25-1.35 to obtain the periplaneta americana extract for treating hepatitis A.
Preferably, the step B is extracted according to the following method, and the coarse powder mixed solution obtained in the step A is subjected to ultrasonic extraction for 3 times:
carrying out ultrasonic extraction for the first time: the temperature is 30-35 ℃, the ultrasonic power is 20-25KHz, ethanol with the concentration of 60% -95% and the weight of 5-8 times that of the crude powder is added, and the extraction time is 30-60 min;
and (3) second ultrasonic extraction: the temperature is 35-40 ℃, the ultrasonic power is 23-28KHz, 60-95% ethanol with the weight of 3-6 times of the weight of the coarse powder is added, and the extraction time is 20-50 min;
carrying out ultrasonic extraction for the third time: the temperature is 40-50 ℃, the ultrasonic power is 25-30KHz, ethanol with the concentration of 60% -95% and the weight of 3-6 times of that of the crude powder is added, and the extraction time is 35-50 min;
more preferably, in step B, the first ultrasound extraction: the temperature is 30-32 ℃, the ultrasonic power is 20-22KHz, ethanol with the concentration of 60% -95% and the weight of 5-8 times of that of the coarse powder is added, and the extraction time is 35-45 min.
More preferably, in step B, the second ultrasonic extraction: the temperature is 35-38 ℃, the ultrasonic power is 23-25KHz, ethanol with the concentration of 75% -95% and the weight of 3-6 times of that of the crude powder is added, and the extraction time is 35-45 min.
More preferably, in step B, the third ultrasonic extraction: the temperature is 43-45 ℃, the ultrasonic power is 28-30KHz, ethanol with the concentration of 80-95% and the weight of 3-6 times of that of the crude powder is added, and the extraction time is 45-50 min.
More preferably, step D: and D, adding purified water with the weight being 7-10 times that of the American cockroach body ethanol concentrated solution obtained in the step C, heating to 85-90 ℃ while stirring, stirring at the constant temperature for 45-60 minutes at the stirring speed of 45-60 revolutions per minute, and then keeping the temperature and standing for 4-5 hours to enable the American cockroach body ethanol concentrated solution to be layered.
The American cockroach extract for treating hepatitis A, which is extracted by the method, is compatible with the following Chinese medicinal materials, has unique curative effect on treating icteric hepatitis A, and the specific compatibility and preparation method are as follows:
the medicine for treating icteric hepatitis A is prepared from the following raw materials in parts by weight: 15-25 parts of oriental wormwood, 15-25 parts of giant knotweed, 5-7 parts of liquorice and 0.15-0.25 part of periplaneta americana extract.
Preferably, the medicament for treating icteric hepatitis A is prepared from the following raw materials in parts by weight: 15-25 parts of oriental wormwood, 15-25 parts of giant knotweed, 5-7 parts of liquorice and 0.2-0.22 part of periplaneta americana extract.
The preparation method of the medicine for treating icteric hepatitis A comprises the following steps: adding 15-25 parts of oriental wormwood, 15-25 parts of giant knotweed rhizome and 5-7 parts of liquorice according to a proportion, extracting by adopting a conventional decoction water extraction or ethanol extraction process, adding the periplaneta americana extract according to a proportion, and uniformly mixing.
The decoction extracted by the preparation method can be directly taken as decoction, or the decoction is concentrated into extract or fluid extract to be directly taken with warm water, or proper auxiliary materials are added to prepare preparations such as tablets, capsules, pills, granules, mixture and the like so as to be convenient to store and take.
Has the advantages that:
1. the periplaneta americana extract for treating hepatitis A extracted by the method has the insoluble matter content of less than 5 percent, the total amino acid content of more than 15.0 percent and the pyrimidine base content of more than 0.1 percent.
2. The periplaneta americana active ingredient prepared by the method has a remarkable effect of curing hepatitis A.
3. When the periplaneta americana extract is used for treating hepatitis A, the regeneration of liver cell tissues can be promoted, the falling of necrotic liver cells is accelerated, damaged liver cells are quickly repaired, and the hepatitis A is cured.
4. The medicament for treating icteric hepatitis A has unique curative effects of short period, quick curative effect, no relapse after treatment and the like on treating hepatitis A.
DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION
Example 1
The medicine for treating hepatitis A extracted from periplaneta americana and the preparation method thereof are realized by adopting the following technology:
A. sieving dried Periplaneta americana body with 10-40 mesh sieve, pulverizing, soaking the obtained Periplaneta americana coarse powder with 60% ethanol until the ethanol liquid level is 10cm higher than the coarse powder liquid level, sealing and storing for one month to obtain coarse powder mixed solution;
B. and B, carrying out ultrasonic extraction on the mixed solution of the coarse powder obtained in the step A for 5 times at the temperature of 50 ℃, wherein the ultrasonic power is as follows: 30KHz, adding 60% ethanol 5 times the weight of the coarse powder when the coarse powder mixed solution is extracted for the first time, wherein the extraction time is 60 min; adding 60% ethanol 5 times the weight of the coarse powder for 40 min; mixing the extractive solutions, and filtering to obtain Periplaneta americana ethanol extractive solution;
C. concentrating the ethanol extract obtained in the step B under reduced pressure under the conditions of vacuum degree of-0.06 MPa and temperature of 50 ℃ until the specific gravity is 1.05 to obtain an ethanol concentrated solution of the periplaneta americana;
D. c, adding purified water which is 7 times of the weight of the American cockroach body ethanol concentrated solution obtained in the step C, heating to 85 ℃ while stirring, stirring for 30 minutes at constant temperature with the stirring speed of 40 revolutions per minute, and then keeping the temperature and standing for 3 hours to layer;
E. and D, discharging the lower-layer water liquid obtained in the step D, filtering the lower-layer water liquid by using filter paper, and concentrating the filtrate under reduced pressure at the temperature of 70 ℃ and the vacuum degree of-0.06 MPa until the specific gravity is 1.25 to obtain the periplaneta americana extract for treating hepatitis A.
The periplaneta americana extract for treating hepatitis A extracted by the method has the insoluble matter content of 3.8 percent, the total amino acid content of 21.2 percent and the pyrimidine base content of 0.3 percent.
The cases are as follows: boy, male, 9 years old. Before half a month, symptoms such as hypodynamia, anorexia, nausea, oil aversion and the like appear, attention is not paid to the symptoms, the symptoms are aggravated, jaundice and slight edema appear, the hepatitis A is confirmed to be treated in hospital for seven days through diagnosis, and the effect is not good. Taking the American cockroach extract of the invention, 0.15g of the American cockroach extract is added into 30ml of warm water and stirred uniformly, and the dosage is one day. The medicine is taken in the morning, noon and evening of each day, 10ml of the medicine is taken each time, the medicine is taken for 7 days continuously, the debilitation, the anorexia, the jaundice, the nausea and the aversion to oil are improved, the medicine is taken for 15 days continuously, all symptoms are completely disappeared, the hepatitis A disease is cured through examination, and the relapse does not occur after the return visit of half a year.
A medicine for treating icteric hepatitis A comprises the following medicines in proportion and a preparation method: adding 15g of oriental wormwood, 15g of giant knotweed rhizome and 5g of liquorice according to a proportion, adding cold water for soaking for 30 minutes, enabling water to be 2-5cm higher than the medicinal materials, soaking, boiling with strong fire, decocting with slow fire for 20 minutes, filtering out medicine residues, decocting for 2 times again, mixing medicine juice obtained by 3 times, adding 0.25g of periplaneta americana extract obtained by the method, and uniformly mixing. The dosage of the medicament is one day, and the medicament is taken in the morning, at noon and at night.
The cases are as follows: li Yi, woman, thirteen years old. Debilitation, anorexia, nausea, emesis, abdominal distention and diarrhea before seven days, especially common cold, liver distending pain before and after one day, yellow staining of sclera and skin. Confirmed icteric hepatitis A. After the medicine for treating icteric hepatitis A is taken for 5 days continuously, the symptoms such as hypodynamia, anorexia, nausea, vomiting, abdominal distension and diarrhea are improved, and after the medicine is taken for 20 days continuously, all symptoms are completely disappeared, the disease is cured, and the disease is not relapsed after the return visit of half a year.
Example 2
The medicine for treating hepatitis A extracted from periplaneta americana and the preparation method thereof are realized by adopting the following technology:
A. sieving dried Periplaneta americana body with 10 mesh sieve, pulverizing, soaking the obtained Periplaneta americana coarse powder with 70% ethanol until the ethanol liquid level is 10cm higher than the coarse powder liquid level, sealing and storing for three months to obtain coarse powder mixed solution;
B. and C, carrying out ultrasonic extraction on the mixed solution of the coarse powder obtained in the step A for 4 times at the temperature of 40 ℃, wherein the ultrasonic power is as follows: 25KHz, adding 70% ethanol 5 times the weight of the coarse powder when the coarse powder mixed solution is extracted for the first time, wherein the extraction time is 50 min; adding 70% ethanol 4 times the weight of the coarse powder for each extraction for 50 min; mixing the extractive solutions, and filtering to obtain Periplaneta americana ethanol extractive solution;
C. concentrating the ethanol extract obtained in the step B under reduced pressure under the conditions of vacuum degree of-0.06 MPa and temperature of 50 ℃ until the specific gravity is 1.05 to obtain an ethanol concentrated solution of the periplaneta americana;
D. c, adding purified water which is 7 times of the weight of the American cockroach body ethanol concentrated solution obtained in the step C, heating to 85 ℃ while stirring, stirring for 30 minutes at constant temperature with the stirring speed of 40 revolutions per minute, and then keeping the temperature and standing for 3 hours to layer;
E. and D, discharging the lower-layer water liquid obtained in the step D, filtering the lower-layer water liquid by using filter paper, and concentrating the filtrate under reduced pressure at the temperature of 70 ℃ and the vacuum degree of-0.06 MPa until the specific gravity is 1.25 to obtain the periplaneta americana extract for treating hepatitis A.
The periplaneta americana extract for treating hepatitis A extracted by the method has the insoluble matter content of 4.2 percent, the total amino acid content of 19.9 percent and the pyridine base content of 0.4 percent.
The cases are as follows: wu, male, 71 years old. The symptoms of hypodynamia, anorexia, nausea, oil aversion and the like appear before the february, attention is not paid to the symptoms, the symptoms are aggravated before three days, jaundice and slight edema appear, the hepatitis A is confirmed by diagnosis, the effect is not good after three days of hospitalization. Taking the periplaneta americana extract of the present invention, 0.25g of the periplaneta americana extract is taken and added into 30ml of warm water to be uniformly stirred, and the dosage is one day. The medicine is taken in the morning, noon and evening of each day, 10ml of the medicine is taken each time, 10 days are continued, the debilitation, the anorexia, the jaundice, the nausea and the aversion to oil are improved, the medicine is taken for 35 days, all symptoms are completely disappeared, the hepatitis A disease is cured through examination, and the relapse does not occur after the return visit of half a year.
A medicine for treating icteric hepatitis A comprises the following medicines in proportion and a preparation method: adding 15g of oriental wormwood, 15g of giant knotweed rhizome and 5g of liquorice according to a proportion, adding cold water for soaking for 30 minutes, enabling water to be 2-5cm higher than the medicinal materials, soaking, boiling with strong fire, decocting with slow fire for 20 minutes, filtering out medicine residues, decocting for 2 times again, mixing medicine juice obtained by 3 times, adding 0.22g of periplaneta americana extract obtained by the method, and uniformly mixing. The dosage of the medicament is one day, and the medicament is taken in the morning, at noon and at night.
The cases are as follows: xu certain, male, 57 years old. Before half a month, debilitation, anorexia, nausea, emesis, abdominal distention and diarrhea, suspected of common cold, are ineffective after treatment, and liver region distending pain appears before and after one day, and sclera and skin appear yellow stain. Confirmed icteric hepatitis A. After the medicine for treating icteric hepatitis A is taken for ten days continuously, the symptoms of hypodynamia, anorexia, nausea, vomiting, abdominal distension and diarrhea are improved, and after the medicine is taken for 20 days continuously, all symptoms are completely disappeared, the disease is cured, and the disease is not relapsed after the return visit of half a year.
Example 3
The medicine for treating hepatitis A extracted from periplaneta americana and the preparation method thereof are realized by adopting the following technology:
A. sieving dried Periplaneta americana body with 10 mesh sieve, pulverizing, soaking the obtained Periplaneta americana coarse powder with 75% ethanol until the ethanol liquid level is 5cm higher than the coarse powder liquid level, sealing and storing for one week to obtain coarse powder mixed solution;
B. and B, carrying out ultrasonic extraction on the mixed solution of the coarse powder obtained in the step A for 3 times:
carrying out ultrasonic extraction for the first time: adding 75% ethanol 5 times the weight of the crude powder at 30 deg.C under ultrasonic power of 20KHz for 30 min;
and (3) second ultrasonic extraction: adding 75% ethanol 3 times the weight of the crude powder at 35 deg.C under ultrasonic power of 23KHz, and extracting for 20 min;
carrying out ultrasonic extraction for the third time: adding 75% ethanol 3 times the weight of the crude powder at 40 deg.C under ultrasonic power of 25KHz for 35 min;
mixing the three extracting solutions, and filtering to obtain an ethanol extracting solution of the periplaneta americana;
C. concentrating the ethanol extract obtained in the step B under reduced pressure under the conditions of vacuum degree of-0.05 MPa and temperature of 50 ℃ until the specific gravity is 1.05 to obtain an ethanol concentrate of the periplaneta americana;
D. adding purified water which is 7 times of the weight of the American cockroach body ethanol concentrated solution obtained in the step C into the American cockroach body ethanol concentrated solution, heating to 85 ℃ while stirring, stirring for 10 minutes at constant temperature with the stirring speed of 20 revolutions per minute, and then keeping the temperature and standing for 1 hour to layer;
E. and D, discharging the lower-layer water liquid obtained in the step D, filtering the lower-layer water liquid by using filter paper, and concentrating the filtrate under reduced pressure at the temperature of 60 ℃ and the vacuum degree of-0.05 MPa until the specific gravity is 1.25 to obtain the periplaneta americana extract for treating hepatitis A.
The periplaneta americana extract for treating hepatitis A extracted by the method has the insoluble matter content of 3.6 percent, the total amino acid content of 22.5 percent and the pyridine base content of 0.2 percent.
The cases are as follows: liu Zhi, female, 5 years old. The symptoms of hypodynamia, anorexia, nausea, oil aversion and the like appear seven days before, attention is not paid to the symptoms, the symptoms are aggravated after two days, jaundice and slight edema appear, the hepatitis A is confirmed to be treated by diagnosis, and the effect is poor after four days of hospitalization. Taking the American cockroach extract of the invention, 0.21g of the American cockroach extract is added into 30ml of warm water and stirred uniformly, and the dosage is one day. The medicine is taken in the morning, noon and evening of each day, 10ml of the medicine is taken each time, 4 days are taken continuously, the symptoms such as hypodynamia, anorexia, jaundice, nausea and oil aversion are improved, the medicine is taken for 7 days, all symptoms are completely disappeared, the hepatitis A disease is cured through examination, and the disease does not relapse after half a year of return visit.
A medicine for treating icteric hepatitis A comprises the following medicines in proportion and a preparation method: adding 15g of oriental wormwood, 15g of giant knotweed rhizome and 5g of liquorice according to a proportion, adding cold water for soaking for 30 minutes, enabling water to be 2-5cm higher than the medicinal materials, soaking, boiling with strong fire, decocting with slow fire for 20 minutes, filtering out medicine residues, decocting for 2 times again, mixing medicine juice obtained by 3 times, adding 0.21g of periplaneta americana extract obtained by the method, and uniformly mixing. The dosage of the medicament is one day, and the medicament is taken in the morning, at noon and at night.
The cases are as follows: wangzhi, female, ten years old. The symptoms of hypodynamia, anorexia, nausea, vomiting, abdominal distension and diarrhea before one month, and suspected cold are not effective after several treatment, the liver area is distended and painful before and after two days, and yellow staining appears on sclera and skin. Confirmed icteric hepatitis A. After the medicine for treating icteric hepatitis A is taken for 3 days continuously, the symptoms such as hypodynamia, anorexia, nausea, vomiting, abdominal distension and diarrhea are improved, and after the medicine is taken for 15 days continuously, all symptoms are completely disappeared, the disease is cured, and the disease is not relapsed after the return visit of half a year.
Example 4
The medicine for treating hepatitis A extracted from periplaneta americana and the preparation method thereof are realized by adopting the following technology:
A. sieving dried Periplaneta americana body with 40 mesh sieve, pulverizing, soaking the obtained Periplaneta americana coarse powder with 80% ethanol until the ethanol liquid level is 10cm higher than the coarse powder liquid level, sealing and storing for 18 months to obtain coarse powder mixed solution;
B. and B, carrying out ultrasonic extraction on the mixed solution of the coarse powder obtained in the step A for 3 times:
carrying out ultrasonic extraction for the first time: adding 80% ethanol 8 times the weight of the crude powder at 35 deg.C under ultrasonic power of 25KHz for 60 min;
and (3) second ultrasonic extraction: adding 80% ethanol 6 times the weight of the crude powder at 40 deg.C under ultrasonic power of 28KHz for 50 min;
carrying out ultrasonic extraction for the third time: adding 80% ethanol 6 times the weight of the crude powder at 45 deg.C under ultrasonic power of 30KHz for 50 min;
mixing the three extracting solutions, and filtering to obtain an ethanol extracting solution of the periplaneta americana;
C. concentrating the ethanol extract obtained in the step B under reduced pressure under the conditions of vacuum degree of-0.06 MPa and temperature of 60 ℃ until the specific gravity is 1.15 to obtain an ethanol concentrated solution of the periplaneta americana;
D. c, adding purified water with the weight being 10 times that of the periplaneta Americana ethanol concentrated solution obtained in the step C, heating to 95 ℃ while stirring, stirring for 60 minutes at constant temperature at the stirring speed of 60 revolutions per minute, and then keeping the temperature and standing for 5 hours to layer;
E. and D, discharging the lower-layer water liquid obtained in the step D, filtering the lower-layer water liquid by using filter paper, and concentrating the filtrate under reduced pressure at the temperature of 80 ℃ and the vacuum degree of-0.06 MPa until the specific gravity is 1.35 to obtain the periplaneta americana extract for treating hepatitis A.
The periplaneta americana extract for treating hepatitis A extracted by the method has the insoluble matter content of 4.1 percent, the total amino acid content of 20.8 percent and the pyridine base content of 0.4 percent.
The cases are as follows: chai chi, male, 15 years old. Nausea, emesis, anorexia, etc. before seven days, and skin yellowing, fever, debilitation, liver pain, etc. The liver was found to be swollen by examination, and the diagnosis was confirmed to be hepatitis A. Taking the American cockroach extract of the invention, 0.15g of the American cockroach extract is added into 30ml of warm water and stirred uniformly, and the dosage is one day. The medicine is taken in the morning, noon and evening of each day, 10ml of the medicine is taken each time, the medicine is taken for 3 days continuously, the fatigue, the anorexia, the jaundice, the nausea and the vomiting are improved, the pain of the liver area is relieved, the medicine is taken for 10 days continuously, all symptoms are completely disappeared, the shape of the liver is recovered to be normal through examination, the hepatitis A disease is cured, and the disease is not relapsed after the medicine is revisited for half a year.
A medicine for treating icteric hepatitis A comprises the following medicines in proportion and a preparation method: adding 18g of oriental wormwood, 18g of giant knotweed rhizome and 7g of liquorice according to a proportion, adding cold water for soaking for 30 minutes, enabling water to be 2-5cm higher than the medicinal materials, soaking, boiling with strong fire, decocting with slow fire for 20 minutes, filtering out medicine residues, decocting for 2 times again, mixing medicine juice obtained by 3 times, adding 0.15g of periplaneta americana extract obtained by the method, and uniformly mixing. The dosage of the medicament is three days, and the medicament is taken in the morning, at noon and at night.
The cases are as follows: lu Yi (Lu Yi in men) 1 year old. Debilitation, anorexia, nausea, emesis, abdominal distention, diarrhea, and yellow skin infection before seven days. Confirmed icteric hepatitis A. After the medicine for treating icteric hepatitis A is taken for 3 days continuously, all symptoms completely disappear, the disease is cured, and the disease does not relapse after the return visit of half a year.
Example 5
The medicine for treating hepatitis A extracted from periplaneta americana and the preparation method thereof are realized by adopting the following technology:
A. sieving dried Periplaneta americana body with 20 mesh sieve, pulverizing, soaking the obtained Periplaneta americana coarse powder with 85% ethanol until the ethanol liquid level is 6cm higher than the coarse powder liquid level, sealing and storing for 12 months to obtain coarse powder mixed solution;
B. and B, carrying out ultrasonic extraction on the mixed solution of the coarse powder obtained in the step A for 3 times:
carrying out ultrasonic extraction for the first time: adding 85% ethanol in an amount which is 5 times the weight of the coarse powder at the temperature of 30 ℃ and the ultrasonic power of 20KHz, and extracting for 35-45 min;
and (3) second ultrasonic extraction: extracting at 35 deg.C with ultrasonic power of 23KHz and ethanol with concentration of 85% and 3 times of the coarse powder for 35 min;
carrying out ultrasonic extraction for the third time: adding 85% ethanol 3 times the weight of the crude powder at 43 deg.C and ultrasonic power of 28KHz, and extracting for 45 min;
mixing the three extracting solutions, and filtering to obtain an ethanol extracting solution of the periplaneta americana;
C. concentrating the ethanol extract obtained in the step B under reduced pressure under the conditions of vacuum degree of-0.05 MPa and temperature of 50 ℃ until the specific gravity is 1.05 to obtain an ethanol concentrate of the periplaneta americana;
D. c, adding purified water which is 7 times of the weight of the American cockroach body ethanol concentrated solution obtained in the step C, heating to 85 ℃ while stirring, stirring for 50 minutes at constant temperature at the stirring speed of 50 revolutions per minute, and then keeping the temperature and standing for 4 hours to layer;
E. and D, discharging the lower-layer water liquid obtained in the step D, filtering the lower-layer water liquid by using filter paper, and concentrating the filtrate under reduced pressure at the temperature of 70 ℃ and the vacuum degree of-0.05 MPa until the specific gravity is 1.25 to obtain the periplaneta americana extract for treating hepatitis A.
The periplaneta americana extract for treating hepatitis A extracted by the method has the insoluble matter content of 4.0 percent, the total amino acid content of 21.3 percent and the pyridine base content of 0.3 percent.
The cases are as follows: chen is certain, male, 25 years old. Three days before, symptoms such as epigastric fullness and discomfort, vomit, anorexia, yellow eyes, scanty yellow urine, constipation and the like appear, the tongue coating is yellow and greasy, the liver area is greatly pressed and painful, the liver swelling is proved by examination, and the hepatitis A is diagnosed. Taking the periplaneta americana extract of the present invention, 0.19g of the periplaneta americana extract is taken and added into 30ml of warm water to be uniformly stirred, and the dosage is one day. The preparation is taken in the morning, noon and evening of each day, 10ml is taken each time, and the preparation is taken for 7 days continuously, the symptoms are improved, and the preparation is taken for 10 days continuously, all symptoms completely disappear, the liver shape is recovered to be normal through examination, the hepatitis A disease is cured, and the disease is not relapsed after the return visit of half a year.
A medicine for treating icteric hepatitis A comprises the following medicines in proportion and a preparation method: adding 20g of oriental wormwood, 20g of giant knotweed rhizome and 6g of liquorice according to a proportion, adding cold water for soaking for 30 minutes, enabling water to be 2-5cm higher than the medicinal materials, soaking, boiling with strong fire, decocting with slow fire for 20 minutes, filtering out medicine residues, decocting for 2 times again, mixing medicine juice obtained by 3 times, adding 0.19g of periplaneta americana extract obtained by the method, and uniformly mixing. The dosage of the medicament is one day, and the medicament is taken in the morning, at noon and at night.
The cases are as follows: he, male, 65 years old. Before March, debilitation, anorexia, nausea, emesis, liver slight distending pain, abdominal distention, diarrhea, and suspected common cold, and after several treatment, sclera and skin yellow stain appear seven days ago. Confirmed icteric hepatitis A. After the medicine for treating icteric hepatitis A is taken for 5 days continuously, the symptoms of hypodynamia, anorexia, nausea, vomiting, slight swelling pain in liver area, abdominal distension and diarrhea are improved, and after the medicine is taken for 10 days, all symptoms are completely disappeared, the disease is cured, and the disease does not relapse after the return visit after half a year.
Example 6
The medicine for treating hepatitis A extracted from periplaneta americana and the preparation method thereof are realized by adopting the following technology:
A. sieving dried Periplaneta americana body with 30 mesh sieve, pulverizing, soaking the obtained Periplaneta americana coarse powder with 95% ethanol until the ethanol liquid level is 10cm higher than the coarse powder liquid level, sealing and storing for 6 months to obtain coarse powder mixed solution;
B. and B, carrying out ultrasonic extraction on the mixed solution of the coarse powder obtained in the step A for 3 times:
carrying out ultrasonic extraction for the first time: adding 95% ethanol 8 times the weight of the crude powder at 32 deg.C under ultrasonic power of 22KHz, and extracting for 45 min;
and (3) second ultrasonic extraction: adding 95% ethanol 6 times the weight of the crude powder at 38 deg.C under ultrasonic power of 25KHz, and extracting for 45 min;
carrying out ultrasonic extraction for the third time: adding 95% ethanol 6 times the weight of the crude powder at 45 deg.C under ultrasonic power of 30KHz for 50 min;
mixing the three extracting solutions, and filtering to obtain an ethanol extracting solution of the periplaneta americana;
C. concentrating the ethanol extract obtained in the step B under reduced pressure under the conditions of vacuum degree of-0.06 MPa and temperature of 60 ℃ until the specific gravity is 1.15 to obtain an ethanol concentrated solution of the periplaneta americana;
D. c, adding purified water with the weight being 10 times that of the periplaneta Americana ethanol concentrated solution obtained in the step C, heating to 90 ℃ while stirring, stirring for 60 minutes at constant temperature at the stirring speed of 60 revolutions per minute, and then keeping the temperature and standing for 5 hours to layer;
E. and D, discharging the lower-layer water liquid obtained in the step D, filtering the lower-layer water liquid by using filter paper, and concentrating the filtrate under reduced pressure at the temperature of 80 ℃ and the vacuum degree of-0.06 MPa until the specific gravity is 1.35 to obtain the periplaneta americana extract for treating hepatitis A.
The periplaneta americana extract for treating hepatitis A extracted by the method has the insoluble matter content of 3.9 percent, the total amino acid content of 19.7 percent and the pyridine base content of 0.4 percent.
The cases are as follows: sunzhi, female, age 35. Taking the periplaneta americana extract of the invention, taking 0.22g of the periplaneta americana extract, adding into 30ml of warm water, and uniformly stirring to obtain a dose of one day. The preparation is taken in the morning, noon and evening of each day, 10ml is taken each time, and the preparation is taken for 3 days continuously, the symptoms are improved, and then the preparation is taken for 7 days, all symptoms completely disappear, and after inspection, the hepatitis A disease is cured, and no recurrence is caused after half a year return visit.
A medicine for treating icteric hepatitis A is prepared by the following steps: 22g of oriental wormwood, 22g of giant knotweed rhizome and 7g of liquorice are taken and crushed into coarse powder, and the medicinal powder is subjected to ultrasonic extraction for 3 times, wherein the ultrasonic extraction conditions are as follows: extracting at 35 deg.C with 90% ethanol 8 times the weight of the crude powder at ultrasonic power of 25KHz for 60min (soaking in ethanol for 30min before first extraction), mixing, and filtering for 3 times to obtain ethanol extractive solution. And (3) concentrating the obtained ethanol extract liquid under reduced pressure at the vacuum degree of-0.05 to-0.06 MPa and the temperature of 50 to 60 ℃ until the specific gravity is 1.35, and adding 0.22g of the American cockroach extract to obtain the medicine for treating icteric hepatitis A.
The cases are as follows: zhangqi, female, age 30. And 3, before seven days, aversion to cold, fever, hypodynamia, anorexia, nausea, vomiting, liver distending pain, abdominal distension and constipation occur, the icteric hepatitis A is diagnosed, the medicine for treating the icteric hepatitis A is taken, the mixed extracted medicine is added into 30ml of warm water and stirred uniformly, and the dosage is one day. The preparation is taken in the morning, noon and evening of each day, 10ml is taken each time, and the preparation is taken for 3 days continuously, the symptoms are improved, and the preparation is taken for 3 days continuously, all symptoms completely disappear, and after inspection, the hepatitis A disease is cured, and no recurrence is caused after half a year return visit.
Example 7
The medicine for treating hepatitis A extracted from periplaneta americana and the preparation method thereof are realized by adopting the following technology:
A. sieving dried Periplaneta americana body with 30 mesh sieve, pulverizing, soaking the obtained Periplaneta americana coarse powder with 90% ethanol until the ethanol liquid level is 8cm higher than the coarse powder liquid level, sealing and storing for 8 months to obtain coarse powder mixed solution;
B. and B, carrying out ultrasonic extraction on the mixed solution of the coarse powder obtained in the step A for 3 times:
carrying out ultrasonic extraction for the first time: adding 90% ethanol 7 times the weight of the crude powder at 31 deg.C under ultrasonic power of 21KHz, and extracting for 40 min;
and (3) second ultrasonic extraction: adding 90% ethanol with 5 times of the coarse powder weight at 36 deg.C and ultrasonic power of 24KHz, and extracting for 40 min;
carrying out ultrasonic extraction for the third time: at 50 deg.C, ultrasonic power of 28KHz, adding 90% ethanol 5 times the weight of the crude powder, and extracting for 50 min;
mixing the three extracting solutions, and filtering to obtain an ethanol extracting solution of the periplaneta americana;
C. concentrating the ethanol extract obtained in the step B under reduced pressure under the conditions of vacuum degree of-0.06 MPa and temperature of 55 ℃ until the specific gravity is 1.05 to obtain an ethanol concentrated solution of the periplaneta americana;
D. c, adding purified water with the weight being 8 times that of the periplaneta Americana ethanol concentrated solution obtained in the step C, heating to 85 ℃ while stirring, stirring for 50 minutes at constant temperature at the stirring speed of 50 revolutions per minute, and then keeping the temperature and standing for 5 hours to layer;
E. and D, discharging the lower-layer water liquid obtained in the step D, filtering by using filter paper, and concentrating the filtrate under reduced pressure at the temperature of 70 ℃ and the vacuum degree of-0.050 MPa until the specific gravity is 1.35 to obtain the periplaneta americana extract for treating hepatitis A.
The periplaneta americana extract for treating hepatitis A extracted by the method has the insoluble matter content of 3.7 percent, the total amino acid content of 20.6 percent and the pyridine base content of 0.3 percent.
The cases are as follows: shen in one, male, 65 years old. The American cockroach extract is taken, 0.25g of American cockroach extract is taken and added into 30ml of warm water to be uniformly stirred, and the dosage is one day. The medicine is taken in the morning, noon and evening of each day, 10ml of the medicine is taken each time, the medicine is taken for 5 days continuously, symptoms of nausea, inappetence, aversion to oil, yellow urine, dry stool and bitter taste are improved, pain of a liver area is relieved, the medicine is taken for 7 days continuously, foot swelling symptoms basically disappear, the medicine is taken for 20 days continuously, all symptoms disappear completely, the hepatitis A disease is cured through examination, and the disease does not relapse after being revisited for half a year.
A medicine for treating icteric hepatitis A is prepared by the following steps: pulverizing 25g of herba artemisiae scopariae, 25g of polygonum cuspidatum and 7g of liquorice into coarse powder, and carrying out ultrasonic extraction on the medicinal powder for 3 times, wherein the ultrasonic extraction conditions are as follows: extracting at 40 deg.C with 30KHz ultrasonic power with 95% ethanol 6 times the weight of the crude powder for 40min, mixing, filtering, and ultrasonic extracting for 3 times (soaking in ethanol for 30min before first extraction). Concentrating the obtained ethanol extractive solution under reduced pressure at 50 deg.C under vacuum degree of-0.05 MPa until the specific gravity is 1.35, and adding 0.25g of the above Periplaneta americana extract to obtain medicine for treating icteric hepatitis A.
The cases are as follows: foramen, age 40. Before february, nausea, aversion to oil, yellow urine, dry stool, bitter taste in mouth and edema of feet are not taken into consideration, sclera and skin are yellow and stained before ten days, and the icteric hepatitis A is diagnosed. The medicine is taken in the morning, noon and evening of each day, 10ml is taken each time, and the medicine is taken for 7 days continuously, the symptoms are improved, and the medicine is taken for 10 days continuously, all the symptoms completely disappear, and the icteric hepatitis A disease is cured after examination, and no relapse occurs after half a year of return visit.
Claims (2)
1. The medicine for treating icteric hepatitis A is characterized by being prepared from the following raw materials in parts by weight: 15-25 parts of oriental wormwood, 15-25 parts of giant knotweed, 5-7 parts of liquorice and 0.2-0.22 part of periplaneta americana extract;
the periplaneta americana extract is prepared by the following method:
A. sieving dried periplaneta americana bodies with a 10-40-mesh sieve, crushing, soaking the obtained periplaneta americana coarse powder in 60-95% ethanol until the liquid level of the ethanol is 5-10 cm higher than the liquid level of the coarse powder, and hermetically storing for more than one week and less than 18 months to prepare a coarse powder mixed solution;
B. and C, carrying out ultrasonic extraction on the mixed solution of the coarse powder obtained in the step A for 3 times at the temperature of 30-50 ℃, wherein the ultrasonic extraction for the first time is as follows: the temperature is 30-32 ℃, the ultrasonic power is 20-22KHz, ethanol with the concentration of 60% -95% and the weight of 5-8 times that of the coarse powder is added, and the extraction time is 35-45 min;
and (3) second ultrasonic extraction: adding 75-95% ethanol in an amount which is 3-6 times the weight of the coarse powder at 35-38 ℃ and under 23-25KHz ultrasonic power, and extracting for 35-45 min;
carrying out ultrasonic extraction for the third time: the temperature is 43-45 ℃, the ultrasonic power is 28-30KHz, ethanol with the concentration of 80-95% and the weight of 3-6 times that of the crude powder is added, and the extraction time is 45-50 min;
mixing the extractive solutions, and filtering to obtain Periplaneta americana ethanol extractive solution;
C. concentrating the ethanol extract obtained in the step B under reduced pressure under the conditions of vacuum degree of-0.05 to-0.06 MPa and temperature of 50 to 60 ℃ until the specific gravity is 1.05 to 1.15 to obtain an American cockroach body ethanol concentrated solution;
D. adding purified water which is 7-10 times of the weight of the American cockroach body ethanol concentrated solution obtained in the step C into the American cockroach body ethanol concentrated solution, stirring and heating to 85-90 ℃, stirring at a constant temperature for 45-60 minutes at a stirring speed of 45-60 revolutions per minute, and then keeping the temperature and standing for 4-5 hours to enable the American cockroach body ethanol concentrated solution to be layered;
E. and D, discharging the lower-layer water liquid obtained in the step D, filtering the lower-layer water liquid by using filter paper, and concentrating the filtrate under reduced pressure at the temperature of 60-80 ℃ and the vacuum degree of-0.05-0.06 MPa until the specific gravity is 1.25-1.35 to obtain the periplaneta americana extract for treating hepatitis A.
2. The medicament for treating icteric hepatitis A according to claim 1, which is prepared by the following steps: adding 15-25 parts of oriental wormwood, 15-25 parts of giant knotweed rhizome and 5-7 parts of liquorice according to a proportion, extracting by adopting a conventional decoction water extraction or ethanol extraction process, adding the periplaneta americana extract according to a proportion, and uniformly mixing.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201611258340.XA CN106619730B (en) | 2016-12-30 | 2016-12-30 | Medicine for treating hepatitis A and preparation method thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201611258340.XA CN106619730B (en) | 2016-12-30 | 2016-12-30 | Medicine for treating hepatitis A and preparation method thereof |
Publications (2)
Publication Number | Publication Date |
---|---|
CN106619730A CN106619730A (en) | 2017-05-10 |
CN106619730B true CN106619730B (en) | 2020-06-16 |
Family
ID=58837610
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201611258340.XA Active CN106619730B (en) | 2016-12-30 | 2016-12-30 | Medicine for treating hepatitis A and preparation method thereof |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN106619730B (en) |
Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1116931A (en) * | 1994-08-17 | 1996-02-21 | 大理医学院 | Ganlong extract and Ganlong preparation |
CN1424052A (en) * | 2003-01-07 | 2003-06-18 | 昆明赛诺制药有限公司 | Medicine for treating chronic hepatitis B |
CN102885858A (en) * | 2012-11-10 | 2013-01-23 | 昆明赛诺制药有限公司 | Method for preparing American cockroach extract for treating hepatitis B |
CN102885856A (en) * | 2012-11-10 | 2013-01-23 | 昆明赛诺制药有限公司 | Production method for producing medicine for treating hepatitis B by periplaneta americana extract |
CA2853734A1 (en) * | 2011-10-28 | 2013-05-02 | University Of Maryland | Methods and compositions related to intracellular neutralization by igg |
CN104288675A (en) * | 2014-09-24 | 2015-01-21 | 黄华 | Traditional Chinese medicine formula for preventing and treating hepatitis A and preparation method thereof |
CN104367594A (en) * | 2013-08-12 | 2015-02-25 | 成都百草和济科技有限公司 | New application of drug residues after alcohol extraction of American cockroach |
CN105055730A (en) * | 2015-09-08 | 2015-11-18 | 山东中医药大学附属医院 | Method for preparing nursing plaster for treating angina |
-
2016
- 2016-12-30 CN CN201611258340.XA patent/CN106619730B/en active Active
Patent Citations (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1116931A (en) * | 1994-08-17 | 1996-02-21 | 大理医学院 | Ganlong extract and Ganlong preparation |
CN1424052A (en) * | 2003-01-07 | 2003-06-18 | 昆明赛诺制药有限公司 | Medicine for treating chronic hepatitis B |
CA2853734A1 (en) * | 2011-10-28 | 2013-05-02 | University Of Maryland | Methods and compositions related to intracellular neutralization by igg |
CN102885858A (en) * | 2012-11-10 | 2013-01-23 | 昆明赛诺制药有限公司 | Method for preparing American cockroach extract for treating hepatitis B |
CN102885856A (en) * | 2012-11-10 | 2013-01-23 | 昆明赛诺制药有限公司 | Production method for producing medicine for treating hepatitis B by periplaneta americana extract |
CN104367594A (en) * | 2013-08-12 | 2015-02-25 | 成都百草和济科技有限公司 | New application of drug residues after alcohol extraction of American cockroach |
CN104288675A (en) * | 2014-09-24 | 2015-01-21 | 黄华 | Traditional Chinese medicine formula for preventing and treating hepatitis A and preparation method thereof |
CN105055730A (en) * | 2015-09-08 | 2015-11-18 | 山东中医药大学附属医院 | Method for preparing nursing plaster for treating angina |
Non-Patent Citations (1)
Title |
---|
中药治疗黄疸型甲肝96例疗效观察;马新 等;《光明中医》;20080131;第23卷(第1期);第13页到15页 * |
Also Published As
Publication number | Publication date |
---|---|
CN106619730A (en) | 2017-05-10 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN109674958B (en) | Traditional Chinese medicine composition with effect of reducing uric acid and preparation method and application thereof | |
CN102008650B (en) | Compound traditional Chinese medicine preparation for treating tumors and preparation method thereof | |
CN103285119B (en) | Chinese medicine composition for treating autoimmune liver disease, and preparation method thereof | |
US20180369308A1 (en) | Chinese herbal medicine composition having a function of relieving hypothyroidism, preparation method and use thereof | |
CN104524441A (en) | Traditional Chinese medicine composition for treating fatty liver hepatitis complicated with hyperlipidemia and preparation method thereof | |
CN104721467B (en) | Traditional Chinese medicine composition for treating diabetic nephropathy and application thereof | |
CN103893410A (en) | Traditional Chinese medicine preparation as well as preparation method and application thereof | |
CN106619730B (en) | Medicine for treating hepatitis A and preparation method thereof | |
CN109692309A (en) | Treat nonalcoholic fatty liver eliminating toxic dampness and heat side | |
CN101693084B (en) | Medical composition for treating epigastric pain yin deficiency syndrome and preparation method thereof | |
CN101011457A (en) | Traditional Chinese medicine composition with immunological enhancement function | |
CN102018903B (en) | Pure traditional Chinese medicine preparation for treating glomerular diseases and preparation method thereof | |
CN104548016A (en) | Application of traditional Chinese medicine composition in preparation of medicine for treating hyperuricemia | |
CN100528186C (en) | Process for preparing Chinese medicine compound injection for treating chronic renal failure and use | |
CN100406026C (en) | Medicine for treating chronic gastritis and gastric carcinoma, and its prepn. method | |
CN101829219B (en) | Periplaneta Americana pharmaceutical composition for treating ulcerative colitis and preparation method thereof | |
CN113209261B (en) | Pharmaceutical composition for treating gastrointestinal tract complex symptom group after radiotherapy and chemotherapy and preparation method and application thereof | |
CN104069173B (en) | Treat pharmaceutical composition of liver diseases and preparation method thereof | |
CN104510857B (en) | A kind of Chinese medicinal effective-part composition for blood fat reducing and preparation thereof | |
CN103751255A (en) | Pachymaran composition for decreasing blood sugar and preparation method thereof | |
KR20170120375A (en) | Chinese medicine preparation | |
CN101926934B (en) | Medicine with protective function on gastric mucosa and liver injury and preparation method thereof | |
CN101757460A (en) | Traditional Chinese medicine preparation for treating gout | |
CN1970025B (en) | Chinese medicinal biological agent of 'Liu Wei Di Huang' decoction and preparation process thereof | |
CN105617294A (en) | Traditional Chinese medicine composition for treating liver cancer |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |