CN106619383A - Face mask containing adipose-derived stem cells and preparation method of face mask - Google Patents
Face mask containing adipose-derived stem cells and preparation method of face mask Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/99—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from microorganisms other than algae or fungi, e.g. protozoa or bacteria
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0212—Face masks
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
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- A61K8/11—Encapsulated compositions
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
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- A—HUMAN NECESSITIES
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/37—Esters of carboxylic acids
- A61K8/375—Esters of carboxylic acids the alcohol moiety containing more than one hydroxy group
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4906—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom
- A61K8/4913—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom having five membered rings, e.g. pyrrolidone carboxylic acid
- A61K8/492—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with one nitrogen as the only hetero atom having five membered rings, e.g. pyrrolidone carboxylic acid having condensed rings, e.g. indol
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61Q19/08—Anti-ageing preparations
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
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- A61K2800/413—Nanosized, i.e. having sizes below 100 nm
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/56—Compounds, absorbed onto or entrapped into a solid carrier, e.g. encapsulated perfumes, inclusion compounds, sustained release forms
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Abstract
The invention discloses a face mask containing adipose-derived stem cells. The face mask comprises an outer hydrophilic fiber layer, a middle function layer and an inner nano-fiber layer. A preparation method of the middle function layer includes the steps: taking 25 parts of adipose-derived stem cells, 10 parts of hyaluronic acid, 30 parts of aloe vera gel, 6 parts of melatonin, 50 parts of deionized water, 80 parts of glycerin and 15 parts of glyceryl stearate according to weight part; mixing the raw materials to prepare mixed solution, enabling the mixed solution to prepare into microcapsules by the aid of a cooling mist spray device, and enabling the microcapsules to be bonded on the outer hydrophilic fiber layer to form the middle function layer. According to the face mask, absorption of face skins can be facilitated, and anti-aging, skin beautifying and anti-anaphylaxis effects of the adipose-derived stem cells are effectively achieved. The invention further provides a preparation method of the face mask.
Description
Technical field
The present invention relates to cosmetics preparation field.It is more particularly related to a kind of face containing fat stem cell
Film and preparation method thereof.
Background technology
Autologous stem cells (stemcells) are the multipotential cells that a class has the of self-replication capacity, under certain condition,
It can be divided into several functions cell.Autologous stem cells (StemCells) are a kind of inabundant differentiation, still jejune thin
Born of the same parents, with the potential function for regenerating various histoorgans and human body, change the countermeasure of human diseases, and medical circle is referred to as "
General-purpose cell ".Autologous fat stem cell is aseptically to take out from human body autologous tissue, separated, after purification in mould
Under the vivo physiological conditions of plan, it is cultivated in vitro by the optimisation technique of core, made the cell not broken up completely not
Disconnected ground differential growth, while the cell of aging is optimized to into fitness form when being infinitely close to 25 years old, and breeds in a large number；Make thin
Born of the same parents keep good growth conditions, and vigorous proliferation activity, meet various treatment needs.
With the gradually maturation of fat stem cell correlation technique, the application in cosmetic field is also more and more extensive.But
It is current, the application of fat stem cell is focused mostly in injection beauty treatment fields, the application in daily cosmetics is urgently for how
Exploitation to be studied.
The content of the invention
It is an object of the invention to solve at least the above, and provide the advantage that at least will be described later.
It is a still further object of the present invention to provide a kind of facial mask containing fat stem cell, it can be dry thin by autologous fat
Born of the same parents are added in facial mask, during unused state, are stored in facial mask in the form of microcapsules；In use, using face
After human body temperature heating, microcapsules rupture discharges the skin lotion containing fat stem cell, so as to promote face skin to absorb,
Effectively play anti-aging, U.S. flesh and the antianaphylactic effect of autologous fat stem cell.
In order to realize these purposes of the invention and further advantage, there is provided a kind of face containing fat stem cell
Film, it includes outer layer hydrophilic fibrage, intermediate functional layer and internal layer layers of nanofibers；Wherein described intermediate functional layer passes through
It is prepared by following methods：Take by weight：The weight portion of fat stem cell 25, the weight portion of hyaluronic acid 10, the weight of Aloe Vera Gel 30
Part, the weight portion of epiphysin 6, the weight portion of deionized water 50, the weight portion of glycerine 80 and the weight portion of stearine 15；Mixing system
Mixed liquor is obtained, microcapsules are obtained by cooling spraying device, the microcapsules are bonded on the outer layer hydrophilic fibrage i.e.
Form the intermediate functional layer.
Preferably, the fat stem cell is autologous fat stem cell, and its preparation method is as follows：
A, human fat tissue block is added normal saline is added in centrifuge tube then using tissue homogenizer process
Add in centrifuge tube into liquid；
B, by the centrifuge tube for filling liquid fat 2400rpm/min centrifugation 5min are placed in a centrifuge, and leave and take bottom deposit
Confluent monolayer cells；
C, adipose tissue digestive pharmaceutical of the bottom deposit confluent monolayer cells from middle addition equivalent is mixed, in being placed in constant temperature oscillator,
In 37 DEG C, 200rpm vibration 5min, 2400rpm/min centrifugation 10min, supernatant liquid is removed, add Dulbecco ' s phosphate
Buffer solution, mixes, and in being transferred to another centrifuge tube, 2400rpm/min centrifugation 5min obtain final product people's fat dry after removal supernatant liquid
Cell.
Preferably, the Aloe Vera Gel is that 100 DEG C of high-temperature sterilization 20min of Jing are obtained under aloe barbadensis Miller Normal juice normal pressure.
Preferably, the outer layer hydrophilic fibrage is made up of nonwoven cloth material, and thickness is 600-800 microns.It is preferred that
For 750 microns.
Preferably, the internal layer layers of nanofibers is made up of nanofiber, and thickness is 80-100 microns.Preferably 85 is micro-
Rice.
Preferably, the hyaluronic acid is 5% hyaluronic acid.
Preferably, the adipose tissue digestive pharmaceutical includes clostridiopetidase A IV types 0.75mg/mL, and trypsase-EDTA is combined
The body 2.5mg/mL and μ l/mL of dimethyl sulfoxide (DMSO) 30, using Dulbecco ' s phosphate buffers as solvent.
It is a further object to provide a kind of preparation method of the facial mask containing fat stem cell, it includes following
Step：
Step one, takes the weight portion of fat stem cell 25, the weight portion of hyaluronic acid 10 and the weight portion of epiphysin 6, and addition is gone
The weight portion of ionized water 50, in gnotobasis, mixing and stirring is obtained mixed liquor one；
Step 2, the weight portion of water-bath glycerine 80 at 50 DEG C, stirring adds the weight portion of stearine 15 and aloe
The weight portion of glue 30；Mixed liquor two is obtained after stable 20min；
Step 3, mixed liquor two is slowly added in mixed liquor two, and stir prepared mixed liquor, adds cooling spray dress
Put, the cooling spraying device is directly sprayed into the mixed liquor on the outer layer hydrophilic fibrage, in outer layer parent
Microcapsules are formed on aqueous fiber layer；The microcapsules are bonded on the outer layer hydrophilic fibrage and form the middle work(
Ergosphere；
Step 4, the internal layer layers of nanofibers is covered on the intermediate functional layer, you can.
Preferably, in the step one, mixed liquor one is preserved in the case where temperature is for 4 DEG C.
Preferably, the thickness of the intermediate functional layer is 500-800 microns.
The present invention at least includes following beneficial effect：Facial mask containing fat stem cell of the present invention, will be containing fat
The liquid of stem cell, it is sealed up for safekeeping received in outer layer hydrophilic fiber layer and internal layer using cooling spraying device in the form of microcapsules
Between rice fibrage.The fat stem cell is sealed up for safekeeping in deionized water, hyaluronic acid and epiphysin mixed liquor, not only can
The activity of the fat stem cell is kept, while the epiphysin and the hyaluronic acid can promote the fat stem cell to enter
Fat differentiation is gone into, always it carries out Osteoblast Differentiation, improve the U.S. flesh effect of facial mask.The Aloe Vera Gel not only can be by the fat
Stem cell is wrapped up so as in low-oxygen environment, suppresses the fat stem cell to carry out Osteoblast Differentiation.Anti-corrosion work can be played simultaneously
With.The internal layer layers of nanofibers is made using nanofiber, and thickness is preferably 85 microns, can promote the fast of intermediate functional layer
Speed infiltration, is easy to face skin to fully absorb.The outer layer hydrophilic fibrage is preferably 750 microns of non-woven fabrics using thickness
Make, the holding of facial heat is conducive in use state, while in unused state, maintaining the low-temperature condition of facial mask.
The further advantage of the present invention, target and feature embody part by description below, and part will also be by this
The research of invention and practice and be understood by the person skilled in the art.
Specific embodiment
With reference to embodiment, the present invention is described in further detail, to make those skilled in the art with reference to specification
Word can be implemented according to this.
It should be appreciated that it is used herein such as " have ", "comprising" and " including " term are not precluded from one or many
The presence or addition of individual other elements or its combination.
Facial mask containing fat stem cell of the present invention, it is characterised in that including outer layer hydrophilic fibrage, middle work(
Ergosphere and internal layer layers of nanofibers；Wherein described intermediate functional layer is prepared by the following method：Take by weight：Fat
The weight portion of stem cell 25, the weight portion of hyaluronic acid 10, the weight portion of Aloe Vera Gel 30, the weight portion of epiphysin 6, the weight of deionized water 50
Part, the weight portion of glycerine 80 and the weight portion of stearine 15；Mixed liquor is mixed to prepare, is obtained by cooling spraying device micro-
Capsule, the microcapsules are bonded on the outer layer hydrophilic fibrage and form the intermediate functional layer.The fat is dry thin
Born of the same parents are sealed up for safekeeping in deionized water, hyaluronic acid and epiphysin mixed liquor, not only can keep the activity of the fat stem cell,
Simultaneously the epiphysin and the hyaluronic acid can promote the fat stem cell to carry out into fat differentiation, and always it carries out skeletonization
Differentiation, improves the U.S. flesh effect of facial mask.The Aloe Vera Gel not only can wrap up the fat stem cell so as in hypoxemia ring
Border, suppresses the fat stem cell to carry out Osteoblast Differentiation.Simultaneously antisepsis can be played.The glycerine and stearine
Adding purpose be the emulsion liquid to form water-in-oil type, be easy in cooling spraying device rapid condensation to form microcapsules.Institute
It is, for example, pressure type cooling sprayer to state cooling spraying device.The hyaluronic acid is, for example, the acid of commercial clear matter.
Wherein in one embodiment, the fat stem cell is autologous fat stem cell, and its preparation method is as follows：
A, human fat tissue block is added normal saline is added in centrifuge tube then using tissue homogenizer process
Add in centrifuge tube into liquid；
B, by the centrifuge tube for filling liquid fat 2400rpm/min centrifugation 5min are placed in a centrifuge, and leave and take bottom deposit
Confluent monolayer cells；
C, adipose tissue digestive pharmaceutical of the bottom deposit confluent monolayer cells from middle addition equivalent is mixed, in being placed in constant temperature oscillator,
In 37 DEG C, 200rpm vibration 5min, 2400rpm/min centrifugation 10min, supernatant liquid is removed, add Dulbecco ' s phosphate
Buffer solution, mixes, and in being transferred to another centrifuge tube, 2400rpm/min centrifugation 5min obtain final product people's fat dry after removal supernatant liquid
Cell.The extracting method of the autologous fat stem cell is fast and convenient, is conducive to keeping the activity of adipocyte, keeps autologous fat
The activity of stem cell.
Wherein in one embodiment, the Aloe Vera Gel is 100 DEG C of high-temperature sterilizations of Jing under aloe barbadensis Miller Normal juice normal pressure
20min is obtained.The Aloe Vera Gel can also be aloe stoste prepared by edible other kind aloes.Such as aloe,
Aloe sinensis and African aloe etc..
Wherein in one embodiment, the outer layer hydrophilic fibrage is made up of nonwoven cloth material, and thickness is 750 micro-
Rice.The outer layer hydrophilic fibrage can keep the facial mask for low-temperature condition in unused state.In use state
When, insulation effect is played, place facial temperature and distribute, promote the microcapsules in intermediate functional layer quickly to dissolve, discharge skin lotion.
Wherein in one embodiment, the internal layer layers of nanofibers is made up of nanofiber, and thickness is 85 microns.Promote
Jing after microcapsules rupture discharge containing fat stem cell rapid osmotic to face skin.
Wherein in one embodiment, the hyaluronic acid is 5% hyaluronic acid.5% hyaluronic acid can be played
98% water retention, promotes absorption and utilization of the face to the liquid containing fat stem cell.
Wherein in one embodiment, the adipose tissue digestive pharmaceutical includes clostridiopetidase A IV types 0.75mg/mL, tryptose
Enzyme-EDTA complexs 2.5mg/mL and μ the l/mL of dimethyl sulfoxide (DMSO) 30, using Dulbecco ' s phosphate buffers as molten
Agent.The adipose tissue digestive pharmaceutical quickly can separate adipocyte from adipose tissue, accelerate fat stem cell
Extraction rate.After the fat stem cell is extracted, can take out when facial mask is prepared and utilize with freezen protective.
The preparation method of the facial mask containing fat stem cell of the present invention, it is comprised the following steps：
Step one, takes the weight portion of fat stem cell 25, the weight portion of hyaluronic acid 10 and the weight portion of epiphysin 6, and addition is gone
The weight portion of ionized water 50, in gnotobasis, mixing and stirring is obtained mixed liquor one；
Step 2, the weight portion of water-bath glycerine 80 at 50 DEG C, stirring adds the weight portion of stearine 15 and aloe
The weight portion of glue 30；Mixed liquor two is obtained after stable 20min；
Step 3, mixed liquor two is slowly added in mixed liquor two, and stir prepared mixed liquor, adds cooling spray dress
Put, the cooling spraying device is directly sprayed into the mixed liquor on the outer layer hydrophilic fibrage, in outer layer parent
Microcapsules are formed on aqueous fiber layer；The microcapsules are bonded on the outer layer hydrophilic fibrage and form the middle work(
Ergosphere；
Step 4, the internal layer layers of nanofibers is covered on the intermediate functional layer, you can.
Wherein in one embodiment, in the step one, mixed liquor one is preserved in the case where temperature is for 4 DEG C.
Wherein in one embodiment, the thickness of the intermediate functional layer is 500-800 microns.
Although embodiment of the present invention is disclosed as above, it is not restricted to listed in specification and embodiment
With, it can be applied to completely various suitable the field of the invention, for those skilled in the art, can be easily
Other modification is realized, therefore under the universal limited without departing substantially from claim and equivalency range, the present invention is not limited
In specific details and shown here as the embodiment with description.
Claims (10)
1. a kind of facial mask containing fat stem cell, it is characterised in that including outer layer hydrophilic fibrage, intermediate functional layer and
Internal layer layers of nanofibers；Wherein described intermediate functional layer is prepared by the following method：Take by weight：Fat stem cell 25
Weight portion, the weight portion of hyaluronic acid 10, the weight portion of Aloe Vera Gel 30, the weight portion of epiphysin 6, the weight portion of deionized water 50, glycerine 80
Weight portion and the weight portion of stearine 15；Mixed liquor is mixed to prepare, microcapsules are obtained by cooling spraying device, it is described
Microcapsules are bonded on the outer layer hydrophilic fibrage and form the intermediate functional layer.
2. the facial mask containing fat stem cell as claimed in claim 1, it is characterised in that the fat stem cell is from body fat
Fat stem cell, its preparation method is as follows：
A, will add normal saline and is then processed into liquid using tissue homogenizer in human fat tissue block addition centrifuge tube
Shape is added in centrifuge tube；
B, by the centrifuge tube for filling liquid fat 2400rpm/min centrifugation 5min are placed in a centrifuge, and leave and take bottom deposit layer thin
Born of the same parents；
C, adipose tissue digestive pharmaceutical of the bottom deposit confluent monolayer cells from middle addition equivalent is mixed, in being placed in constant temperature oscillator, in 37
DEG C, 200rpm vibration 5min, 2400rpm/min centrifugation 10min, remove supernatant liquid, add Dulbecco ' s phosphate-buffereds
Liquid, mixes, and in being transferred to another centrifuge tube, 2400rpm/min centrifugation 5min obtain final product people's fat dry thin after removal supernatant liquid
Born of the same parents.
3. the facial mask containing fat stem cell as claimed in claim 2, it is characterised in that the Aloe Vera Gel is aloe barbadensis Miller
100 DEG C of high-temperature sterilization 20min of Jing are obtained under Normal juice normal pressure.
4. the facial mask containing fat stem cell as claimed in claim 3, it is characterised in that the outer layer hydrophilic fibrage is
Nonwoven cloth material is made, and thickness is 600-800 microns.
5. the facial mask containing fat stem cell as claimed in claim 4, it is characterised in that the internal layer layers of nanofibers is to receive
Rice fiber is made, and thickness is 80-100 microns.
6. the facial mask containing fat stem cell as claimed in claim 1, it is characterised in that the hyaluronic acid is 5% transparent
Matter acid.
7. the facial mask containing fat stem cell as claimed in claim 2, it is characterised in that the adipose tissue digestive pharmaceutical is included
Clostridiopetidase A IV types 0.75mg/mL, trypsase-EDTA complexs 2.5mg/mL and the μ l/mL of dimethyl sulfoxide (DMSO) 30, use
Dulbecco ' s phosphate buffers are used as solvent.
8. the preparation method of the facial mask containing fat stem cell as described in any one of claim 1-7, it is characterised in that include
Following steps：
Step one, takes the weight portion of fat stem cell 25, the weight portion of hyaluronic acid 10 and the weight portion of epiphysin 6, adds deionization
The weight portion of water 50, in gnotobasis, mixing and stirring is obtained mixed liquor one；
Step 2, the weight portion of water-bath glycerine 80 at 50 DEG C, stirring adds the weight portion of stearine 15 and Aloe Vera Gel 30
Weight portion；Mixed liquor two is obtained after stable 20min；
Step 3, mixed liquor two is slowly added in mixed liquor two, and stir prepared mixed liquor, adds cooling spraying device,
The cooling spraying device is directly sprayed into the mixed liquor on the outer layer hydrophilic fibrage, in the outer layer hydrophilic
Microcapsules are formed on fibrage；The microcapsules are bonded on the outer layer hydrophilic fibrage and form the intermediate function
Layer；
Step 4, the internal layer layers of nanofibers is covered on the intermediate functional layer, you can.
9. the preparation method of the facial mask containing fat stem cell as claimed in claim 8, it is characterised in that the step one
In, mixed liquor one is preserved in the case where temperature is for 4 DEG C.
10. the preparation method of the facial mask containing fat stem cell as claimed in claim 8, it is characterised in that the middle work(
The thickness of ergosphere is 500-800 microns.
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| CN201710013268.2A CN106619383A (en) | 2017-01-09 | 2017-01-09 | Face mask containing adipose-derived stem cells and preparation method of face mask |
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| CN201710013268.2A CN106619383A (en) | 2017-01-09 | 2017-01-09 | Face mask containing adipose-derived stem cells and preparation method of face mask |
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