CN106617097A - Health-care food and preparation method thereof - Google Patents
Health-care food and preparation method thereof Download PDFInfo
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- CN106617097A CN106617097A CN201710127572.XA CN201710127572A CN106617097A CN 106617097 A CN106617097 A CN 106617097A CN 201710127572 A CN201710127572 A CN 201710127572A CN 106617097 A CN106617097 A CN 106617097A
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- eucommia ulmoides
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- 238000002360 preparation method Methods 0.000 title claims abstract description 31
- 235000013305 food Nutrition 0.000 title abstract 3
- 239000000284 extract Substances 0.000 claims abstract description 47
- 241000208689 Eucommia ulmoides Species 0.000 claims abstract description 42
- 235000015097 nutrients Nutrition 0.000 claims abstract description 11
- 210000000936 intestine Anatomy 0.000 claims abstract description 5
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 31
- 230000001954 sterilising effect Effects 0.000 claims description 27
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 22
- 239000001963 growth medium Substances 0.000 claims description 22
- 238000000605 extraction Methods 0.000 claims description 20
- 241000208688 Eucommia Species 0.000 claims description 17
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 16
- 239000012530 fluid Substances 0.000 claims description 16
- 239000001888 Peptone Substances 0.000 claims description 15
- 108010080698 Peptones Proteins 0.000 claims description 15
- 235000015278 beef Nutrition 0.000 claims description 15
- 235000019319 peptone Nutrition 0.000 claims description 15
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 13
- 239000008103 glucose Substances 0.000 claims description 12
- 239000011780 sodium chloride Substances 0.000 claims description 11
- 238000010438 heat treatment Methods 0.000 claims description 10
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
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- 238000004108 freeze drying Methods 0.000 claims description 6
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- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 4
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- 238000005728 strengthening Methods 0.000 abstract description 5
- 240000001046 Lactobacillus acidophilus Species 0.000 abstract description 4
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- 229940039695 lactobacillus acidophilus Drugs 0.000 abstract description 4
- 230000006870 function Effects 0.000 abstract description 3
- 210000002784 stomach Anatomy 0.000 abstract description 3
- 239000004480 active ingredient Substances 0.000 abstract 2
- 241000194032 Enterococcus faecalis Species 0.000 abstract 1
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- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 1
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- 241000588921 Enterobacteriaceae Species 0.000 description 1
- 208000003618 Intervertebral Disc Displacement Diseases 0.000 description 1
- 208000012659 Joint disease Diseases 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 206010024453 Ligament sprain Diseases 0.000 description 1
- 206010061307 Neck deformity Diseases 0.000 description 1
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- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000012490 blank solution Substances 0.000 description 1
- 229960005069 calcium Drugs 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- MKJXYGKVIBWPFZ-UHFFFAOYSA-L calcium lactate Chemical compound [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 description 1
- 239000001527 calcium lactate Substances 0.000 description 1
- 229960002401 calcium lactate Drugs 0.000 description 1
- 235000011086 calcium lactate Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
- 238000005660 chlorination reaction Methods 0.000 description 1
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- RTZKZFJDLAIYFH-UHFFFAOYSA-N ether Substances CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 1
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- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
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- 230000036449 good health Effects 0.000 description 1
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- 239000005556 hormone Substances 0.000 description 1
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- 229920002674 hyaluronan Polymers 0.000 description 1
- 229960003160 hyaluronic acid Drugs 0.000 description 1
- 201000010930 hyperostosis Diseases 0.000 description 1
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- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
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- 239000008101 lactose Substances 0.000 description 1
- 230000009247 menarche Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
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- 239000011707 mineral Substances 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
Abstract
The present invention provides health-care food prepared by conducting probiotic culture of a fermentation extract of eucommia ulmoides barks and eucommia ulmoides leaves and a preparation method thereof. By selecting lactobacillus acidophilus and enterococcus faecalis in members of probiotics and combining a scientific formula and deep culture fermentation, the advantages of the two bacteria are combined and two-way fermentation is conducted. Purely natural nutrients and bio-active ingredients in the eucommia ulmoides meet the needs of the growth and reproduction of the probiotics, and the fermentation process further decomposes the own nutrients and bio-active ingredients in the eucommia ulmoides, so that the health-care food plays the functions of the probiotics in strengthening intestines and stomach and the health-care functions of the eucommia ulmoides in strengthening tendons and bones.
Description
Technical field
The present invention relates to a kind of fermentation extract with Bark of Eucommia Ulmoides and folium cortex eucommiae is carried out obtained by probioization culture
Health food and preparation method thereof, it can be applicable to the health care of the diseases such as old gonarthromeningitis and osteoarthrosis and health
It is multiple.
Background technology
Synovitis（English name synovitis）It is a kind of multiple diseases, synovitis is because microcirculation is not freely caused
Aseptic inflammation, cause juice to lack of proper care, formed hydrops a kind of arthropathy；Due to gonarthromeningitis, extensively it is located at
The shallower position in limbs surface, therefore damaged and the chance that infects is more, middle-young patients are mainly sprain of knee joint and various
Intraarticular damage and cause, middle-older patient, to be secondary to knee joint endoprosthesis scorching more, mainly because of cartilage degeneration and hyperostosis
It is raw, mechanicalness biochemistry sexual stimulus is produced, excite knee joint synovial membrane oedema, ooze out hydrops.
Gonarthromeningitis, cardinal symptom based on arthralgia, with mild swelling, walk creak creak snap, on
Go downstairs or firmly when arthralgia or have sense of discomfort, long-time walking joint has fever to send out a stiff sensation, and the pain increased.At present
For gonarthromeningitis treatment despite many effective treatment methods；Wherein, the traditional Chinese medical science is mainly external application therapy, massages
Method, traditional Chinese medical science plaster therapy punctures acupuncture and moxibustion therapy.Doctor trained in Western medicine mainly adopts hormone, drawing liquid, flushing, antibiotic；Newest therapy is to disease
Stove injection location hyaluronic acid nutrient solution.Even so, still also have many patients to cure, the folding for bearing the pain for a long time
Mill, affects patient's normal life, and to work many troubles are brought, or even has psychologically also coverd with one layer of shade, it is believed that synovial membrane
Inflammation is a kind of incurable disease.
For above-mentioned situation, the present invention does one's utmost to seek a kind of substitute for gonarthromeningitis patient and substitutes rehabilitation skill
Art；Laboratory sample is not expected in the early stage in clinical practice, discovery not only has positive effect to gonarthromeningitis patient, and to bone
Matter osteoporosis patient, or even the effect for making patient satisfaction is also occurred in that to waist intervertebral ridge disk protrusion patient.
The content of the invention
The invention provides a kind of fermentation extract with Bark of Eucommia Ulmoides and folium cortex eucommiae is carried out obtained by probioization culture
Health food and preparation method thereof, it is matched somebody with somebody by the lactobacillus acidophilus and intestines streptococcus in screening probio member, in addition science
Side and deep drainpipe fermentation, both have complementary advantages and bi-directional fermentation, pure natural nutrient content and bioactive ingredients in the bark of eucommia
Both the demand of growth of probiotics breeding had been met, the process of its fermentation further degrades bark of eucommia idiotrophic and biologically active
Composition, so as to play the health care of the strong stomach of probio and bark of eucommia strengthening the bones and muscles.
The present invention realizes that above-mentioned purpose is employed the following technical solutions：
A kind of health products and preparation method thereof, it adopts Bark of Eucommia Ulmoides and folium cortex eucommiae with 1:2 weight ratio carries out extraction concentration, then
The nutriments such as addition peptone, beef extract, glucose, sodium chloride make corresponding nutrient solution, are inoculated with nutrient solution prebiotic
Bacterium is fermented, and finally terminal nutrient solution is dried prepared.
A kind of health products and preparation method thereof, its concrete technology flow process is as follows：
First, the preparation of culture medium
1st, the preparation of one-level, secondary medium
Raw material：Peptone 1-4%, beef extract 0.1-0.5%, glucose 1-4%, sodium chloride 0.1-0.5%, eucommia leaf extract 1-
4%th, calcium carbonate 1-2%, pure water 86-95.8%.Above proportioning is weighed according to proportion by weight.
Described bark of eucommia eucommia leaf extract be by weight ratio be 1:The pure water extractions of Jing after 1 folium cortex eucommiae and pure water infiltration
Take, concentrate, be spray-dried gained.
Step：By above-mentioned formula precise, and sequentially add plus heat container in, add part metering pure water, plus
Heat makes material all dissolve, and with the HCI of 20%NaoH and 2mol/l pH value 6.5-6.8 is adjusted, and finally supplies the pure of surplus
Water, is carefully dispensed after stirring, and is placed in the sterilizing of electrothermal pressure sterilizer, controls 115 DEG C -121 DEG C of sterilising temp, sterilizing
Time 25-30min, after slant tube sterilizing, sets while hot in time inclined-plane, and sterilizing material is all down to after room temperature that to turn refrigerator cold
Hide, it is standby for inoculation and transferred species.
2nd, three-level, the preparation of level Four culture medium
Raw material：Folium cortex eucommiae, Bark of Eucommia Ulmoides, peptone, beef extract, glucose, sodium chloride, calcium carbonate, pure water.
Step：
1）The pretreatment of raw material, Bark of Eucommia Ulmoides is carried out processing the fragment for being broken into 1-2 centimetre of size；And pluck in annual drop frost
Fresh folium cortex eucommiae Jing instantaneous high-temperatures or the sun before is exposed to the sun, complete dezymotize pack after acceptance(check) it is standby；
2）Bark of Eucommia Ulmoides of weighing respectively is a, and two parts of folium cortex eucommiae is placed in two rustless steel containers respectively by 1：1 ratio, adds pure
Water purification is infiltrated, and Bark of Eucommia Ulmoides infiltrates 8 hours, and folium cortex eucommiae infiltrates 2 hours；
3）Extract：The folium cortex eucommiae for having infiltrated is transferred to the extractor extracted in thickener unit, then by 1：6 and 1：4 ratio
Plus pure water is extracted twice, for the first time 70 DEG C -80 DEG C of Extracting temperature of setting, 1 hour extraction time, arrange extract for the second time
Temperature is 70 DEG C -80 DEG C, 1 hour extraction time；
The Bark of Eucommia Ulmoides for having infiltrated is transferred into extractor, by 1:4 and 1:3 ratio adds pure water to carry out heating extraction twice；
For the first time 98 DEG C -100 DEG C of Extracting temperature of setting, 1 hour extraction time, second 98 DEG C -100 DEG C of Extracting temperature of setting, extract
1 hour time, merge extract twice and uniformly mix with bark of eucommia leaf extract；
4）Above-mentioned mixed uniformly extract is transferred into concentration by the filter divided into extraction thickener unit to fill, is started
Vacuum-concentrcted device, sets 65 DEG C -75 DEG C of thickening temperature, vacuum 0.06-0.08MPa, concentrate specific gravity control 1.10-
1.12；
（5）Folium cortex eucommiae and Bark of Eucommia Ulmoides extract concentrate, the formula and preparation process of culture medium：Collect above-mentioned proportion 1.10-1.12
Extract, accurate overall stoichiometric product is placed in stainless steel plus heat container, sequentially adds peptone 1-2%, beef extract 0.1-0.2%,
Sodium chloride 0.1-0.2%, glucose 1-2%, treat peptone, beef extract, sodium chloride, and glucose is all after dissolving, with 20%NaoH and
2Hcl adjusts pH value 6.5-6.8, is eventually adding the calcium carbonate of 0.5-1.0%, and 115 ~ 121 DEG C of sterilising temp, sterilizing are set after sealing
28 ~ 30min of time, is cooled to 37 DEG C ± 2 DEG C after sterilizing.
2nd, probio inoculation
The probio for being adopted is lactobacillus acidophilus or intestines streptococcus.
Bacterial classification is gone into first cell culture medium from slant strains, and go to two grades successively, three-level, level Four culture medium to be to expand life
Produce, described first cell culture medium and the cultivation temperature of secondary medium is 37 DEG C ± 2 DEG C, and incubation time is 22-24 hours；
Described three-stage culture medium and the cultivation temperature of level Four culture medium is 37 DEG C -39 DEG C, and incubation time is 18-20 hours.
When terminal PH of zymotic fluid in culture medium is 3.8 ~ 4.2, you can zymotic fluid is transferred into next link.
3rd, it is dried
1st, it is spray-dried
The above-mentioned zymotic fluid for fermenting is connected into the peristaltic pump of dryer group, 160 ~ 180 DEG C of EAT, leaving air temp 65 is set
, dry powder is collected in time, seal the filling capsule of stored refrigerated confession quality inspection compressing tablet standby by ~ 75 DEG C.
2nd, freeze-drying
By the zymotic fluid for fermenting, in environment purification, lyophilized plate is dispensed, 120 points of pre-freeze at the temperature started shooting below -30 DEG C
Clock, is then once distilled, defrost discharging after parsing-desiccation, seals the filling capsule of stored refrigerated confession quality inspection compressing tablet standby.
The present invention is had the advantages that using above-mentioned technical proposal：
Probio is inoculated into Bark of Eucommia Ulmoides and bark of eucommia leaf extract, its abundant mineral matter, amino acid, carbohydrate contain
Amount, had both met the needs of growth of probiotics breeding, the bioactive ingredients wood lipidol class in Bark of Eucommia Ulmoides leaf, Phenylpropanoid Glycosides class, ring
Alkene ether terpene, flavonoids, various secondary metabolites are further degraded, and are more beneficial for absorption of human body, are newly produced in sweat
Raw soluble calcium lactate and mannatide, enhance gonarthromeningitis and osteoarthrosis patient anti-inflammatory and the work replenished the calcium
With；The result of bi-directional fermentation has more highlighted bark of eucommia strengthening the bones and muscles, the function of reparation cartilaginous tissue and probio adjustment enterobacteriaceae and has put down
Weighing apparatus, suppresses enteron aisle corruption bacteria growing to guarantee the good health status of human gastrointestinal tract, is the normal performance of each organ of human body, bone
Stably play good rehabilitation and health-care effect.
Description of the drawings
Fig. 1 is the related form of quality measurements.
Fig. 2 is relative recording of the patient using the effect of this product.
Specific embodiment
With reference to specific embodiment, the present invention will be further described.
I type dried frozen aquatic products embodiment 1
Probio inclined-plane and one-level test tube, the preparation of two grades of triangular flask fermentation seed liquids：（The I type Product Activity probio containing detection
The culture medium of content）
1st, the preparation of probio blank cultures：Prepare cumulative volume 2000mL.Weigh peptone 20g, beef extract 10g, sodium chloride
6g, eucommia leaf extract 20g, lactose 20g, calcium carbonate 1.0%（Inclined-plane and the another Ensure Liquid agar 1.5%- of detection viable bacteria culture medium
2.0%）.The above-mentioned formula materials precise in addition to calcium carbonate is put in one plus heat container successively, is initially charged 1500mL pure
Water, after heating for dissolving, with the HCI solution of 20%NaoH and 2mol/l PH6.5-6.8 is adjusted, and supplements purified water remainder to cumulative volume
Stir for 2000mL, packing slant tube 6mL/ × 8, one-level test tube 9mL/ × 8, two grades of triangular flask 175mL/
Only × 8, remaining 400mL, packing detection product viable bacteria culture medium；100mL/ × 4 bottles, per bottle of another Ensure Liquid agar 1.5g
And calcium carbonate（Lightweight）1g.Packing is jumped a queue respectively after finishing and wraps Tie, puts electric-heating steam sterilizer, sets 115-118 DEG C of sterilising temp,
Sterilization time 25min, except 8 inclined-planes set while hot inclined-plane, puts together refrigerator cold-storage standby after remaining sterilizing article is down to room temperature
With.
2nd, the preparation of probio slant strains and 1-2 level fermentation mediums（Step operation ultra-clean work in clean area
Platform is carried out）；Take original probio bacterial classification（Come from Chinese microorganism strain collection）1 → turn 2 one-level blank solution examinations
In managing → putting the one-level test tube fluid nutrient medium of constant incubator → 37 ± 2 DEG C active culture 22-24 hour → again from after activation
Lined in blank inclined-plane culture pipe using oese → 37 DEG C of ± 2 DEG C of culture 22-24 hours observe well-grown inclined-plane →
37 DEG C of ± 2 DEG C of culture 22-24 hours in one-level blank cuvette fluid nutrient medium of transferring observe well-grown one-level test tube training
Foster base transfers in two grades of triangular flask blank cultures → 37 DEG C of ± 2 DEG C of culture 22-24 hours finally by second order fermentation culture medium,
Follow-up 5000ml three-level round-bottomed flask 3000ml and level Four 50L stainless steel fermentation tank are inoculated in as the seed liquor that is prepared in advance
In blank cultures after 30L sterilizings.
3rd, 5000mL three-levels round-bottomed flask（3000ml）The preparation of culture medium：
1. Bark of Eucommia Ulmoides 1.5kg is weighed respectively, folium cortex eucommiae 3kg simultaneously presses respectively 1:1 ratio, plus pure water infiltration Bark of Eucommia Ulmoides infiltration 8
Hour, folium cortex eucommiae infiltrates 2 hours；
2. by the above-mentioned folium cortex eucommiae for infiltrating and Bark of Eucommia Ulmoides respectively by 1:6 and 1:4 ratios add pure water to be extracted twice, the bark of eucommia
Leaf sets 70-80 DEG C of Extracting temperature, extraction time 1:00 hour；
3. the extract Jing vacuum decompression filters for merging above-mentioned Bark of Eucommia Ulmoides and folium cortex eucommiae are transferred to reduced pressure concentration tank, if vacuum
0.06 ~ 0.08Mpa, sets 65 ~ 75 DEG C of thickening temperature, and concentration cumulative volume is about 8000ml, and proportion is 1.10-1.12；
4. collect above-mentioned concentrate to put in the stainless steel plus heat container of a cleaning, peptone 90g, beef extract 9g, chlorine are supplemented while hot
Change sodium 9g, glucose 90g, after heating for dissolving, with the HCI of 20%NaoH and 2mol/l PH6.5 ~ 6.8 are adjusted, finally supplement pure
Water cumulative volume is 9000ml, in being carefully sub-packed in the clean round-bottomed flask of 5000ml, 3000ml is dispensed per only bottle, then respectively toward often
In bottle plus precipitated calcium carbonate 30g, sealing plus to plug and close air bleeding valve after electric-heating steam sterilizer electrified regulation 10min, setting is gone out
118 ~ 121 DEG C of bacterium temperature, after sterilization time 30min, exhaust decompression zero wears asbestos gloves and opens lid on electrothermal sterile device,
Gently 3 round-bottomed flasks are transferred into superclean bench, after being down to room temperature, according to sterile working program, by 3 two grades of triangular flasks
（Every bottle of 175ml）The seed liquor for fermenting, during 3 three-level round-bottomed flasks are inoculated in respectively, in time by the calcium carbonate at jar,precipitating bottom
Uniformly shake and be transferred to automatic constant-temperature culture shaking table, set 37 ~ 39 DEG C of cultivation temperature, 105 ~ 115/min of wobble frequency, training
Hair care ferment 16-18 hours, observation bottom of bottle calcium carbonate is almost wholly absent, and zymotic fluid color is from depth to shallow and smell micro-perfume, you can
Turning freeze-drying unit carries out freeze-drying.3 round-bottomed flask cumulative volume 9000ml, are uniformly sub-packed in 10 stainless steel lyophilized plate
In, every disk about 900ml.
Setting freeze drier relevant parameter, the 120 minutes → once distillation → parsing-desiccation → shutdown of less than -30 DEG C pre-freezes
Defrost discharges → seals stored refrigerated.It is standby for quality inspection and the filling capsule of compressed tablets.
I type dried frozen aquatic products embodiment 2：
1st, Bark of Eucommia Ulmoides 5kg, folium cortex eucommiae 10kg are weighed respectively, and respectively by 1:1 ratio adds pure water to be infiltrated, Bark of Eucommia Ulmoides
Infiltration 8 hours, folium cortex eucommiae infiltrates 2 hours.
2nd, by the above-mentioned folium cortex eucommiae for infiltrating and Bark of Eucommia Ulmoides, respectively by 1:6 and 1:4 ratio adds pure water to carry out respectively
Extract twice, folium cortex eucommiae setting Extracting temperature 75 ~ 80,1 hour extraction time, 90 ~ 100 DEG C of Bark of Eucommia Ulmoides Extracting temperature, during extraction
Between 1:30 hours, merge folium cortex eucommiae and Bark of Eucommia Ulmoides extract twice, Jing vacuum decompression filters are transferred to reduced pressure concentration tank, if
Determine 0.06 ~ 0.08Mpa of vacuum, 65 ~ 75 DEG C of thickening temperatures of setting and be concentrated into cumulative volume and be about 28L proportion 1.10-1.12 to be transferred to
50L stainless steel seed fermentation tanks.
3rd, open seeding tank charge door and proceed to mixing extraction concentrate, peptone 300g, beef extract 30g, chlorine are supplemented while hot
Change sodium 30g, glucose 300g, start agitating heating dissolving, adjust PH6.5 ~ 6.8 with the HCI of 20%NaoH and 2mol/l, finally
It is that 30L adds again calcium carbonate 300g toward in tank to supplement pure water to cumulative volume, closes charge door, starts electrical heating, setting sterilizing
118 ~ 121 DEG C of temperature, after sterilization time 30min, opens cooling water valve, and when temperature in tank is down into 37 DEG C ~ 39 DEG C, opening connects
Mouth is planted, alcohol pyrosphere is lighted, preprepared three-level round-bottomed flask probiotics fermention seed liquor 3000ml is inoculated with（Inoculum concentration is
10%）It is rapid to close inoculation mouth, the setting fermentation beginning and ending time, 37 ~ 39 DEG C of cultivation and fermentation temperature is set, fermentation time 18 ~ 20 is little
When.During zymotic fluid PH3.8 ~ 4.2, from depth to shallow, smell micro-perfume judges that fermentation termination shifts in time cold to observation zymotic fluid color
Lyophilizer group carries out freeze-drying and sets relevant parameter, the distillation → parsing-desiccation of less than -30 DEG C pre-freezes 120 minutes → once
→ defrost discharging is shut down, sealing refrigeration preserves standby for detection and the filling capsule of compressed tablets.
II type dry product embodiment 1
The preparation of three-level round-bottomed flask culture medium（The short neck 5000ml of round-bottomed flask specification）
The actual capacity of preparing of every round-bottomed flask is 3000ml, and 3 are prepared altogether, and cumulative volume is 9000ml.
1st, Bark of Eucommia Ulmoides 1.5kg, folium cortex eucommiae 3kg are weighed respectively, and respectively by 1:1 ratio, plus pure water infiltration, Bark of Eucommia Ulmoides
Infiltration 8 hours, folium cortex eucommiae infiltrates 2 hours；
2nd, by the above-mentioned folium cortex eucommiae for infiltrating and Bark of Eucommia Ulmoides, respectively by 1:6 and 1:4 ratio adds pure water, enters at twice respectively
Row is extracted, folium cortex eucommiae 65 ~ 75 DEG C of Extracting temperature of setting, 1 hour extraction time.98 ~ 100 DEG C of the Extracting temperature of Bark of Eucommia Ulmoides setting,
Extraction time 1:30 hours；
3rd, above-mentioned folium cortex eucommiae and Bark of Eucommia Ulmoides extract twice is merged, Jing vacuum filters are transferred to reduced pressure concentration tank, and setting is true
0.06 ~ 0.08Mpa of reciprocal of duty cycle sets 65 ~ 75 DEG C of thickening temperature, and when being concentrated into cumulative volume about 8000ml, proportion is 1.10 ~ 1.12；
4th, collect above-mentioned concentrate to put in the stainless steel plus heat container of cleaning, peptone 90g, beef extract 9g, chlorination are supplemented while hot
Sodium 9g, with HCI solution adjustment PH6.5 ~ 6.8 of 20%NaoH and 2mol/l after heating for dissolving, finally supplementing pure water cumulative volume is
9000ml, in being carefully sub-packed in the clean round-bottomed flask of 3 5000ml, per only dispensing 3000ml, then respectively toward bottle plus lightweight
Calcium carbonate 30g, sealing is jumped a queue, and after putting electric-heating steam sterilizer electrified regulation exhaust 10min, closes air bleeding valve, setting sterilizing temperature
118 ~ 121 DEG C of degree, sterilization time 30min, exhaust zero is worn and covered 3 circles gently on asbestos gloves opening electrothermal sterile device
Bottom flask is transferred to superclean bench, after being down to room temperature, according to sterile working program, by 3 preprepared two grades of triangles
Bottle（Every bottle of 175ml/ is only）The seed liquor for fermenting, during 3 round-bottomed flasks are inoculated in respectively, will be deposited in time the lightweight of bottom of bottle
Calcium carbonate has uniformly shaken, you can be transferred to automatic constant-temperature culture shaking table, sets 37 ~ 39 DEG C of cultivation temperature, and wobble frequency 105 ~
110/min, cultivation and fermentation 16 ~ 18 hours, observation bottom of bottle calcium carbonate be almost wholly absent, zymotic fluid color from depth to shallow, smell
Micro-perfume, you can proceed to spray drying unit and be spray-dried, connect the pipeline being spray-dried and between round-bottomed flask, start compacted
Dynamic pump, sets dryer group operation sequence and sets 160 ~ 180 DEG C of EAT, and 65 ~ 75 DEG C of leaving air temp collects in time spraying
It is dried dry product sealing stored refrigerated, it is standby for detection and the filling capsule of compressed tablets.
II type dry product embodiment 2
1st, Bark of Eucommia Ulmoides 5kg, folium cortex eucommiae 10kg are weighed respectively, and respectively by 1:1 ratio adds pure water to be infiltrated, Bark of Eucommia Ulmoides
Infiltration 8 hours, folium cortex eucommiae infiltrates 2 hours；
2nd, by the above-mentioned folium cortex eucommiae for infiltrating and Bark of Eucommia Ulmoides, respectively by 1:6 and 1:4 ratio adds pure water, and two are carried out respectively
Secondary extraction, folium cortex eucommiae 75 ~ 80 DEG C of Extracting temperature of setting, 1 hour extraction time.98 ~ 100 DEG C of Bark of Eucommia Ulmoides Extracting temperature, during extraction
Between 1:30 hours, merge folium cortex eucommiae and Bark of Eucommia Ulmoides extract twice, Jing vacuum decompression filters are transferred to reduced pressure concentration tank, set
0.06 ~ 0.08Mpa of vacuum, sets 65 ~ 75 DEG C of thickening temperature, is concentrated into cumulative volume 28L or so, proportion 1.10-1.12.Transfer
To 50L stainless steel seed fermentation tanks；
3rd, seeding tank charge door is opened, takes advantage of mixed extract and still vulcanize and fill peptone 300g, beef extract 30g, sodium chloride 30g, Portugal
Grape sugar 300g, starts stirring, and heating for dissolving adjusts PH6.5 ~ 6.8, finally supplements pure with the HCI solution of 20%NaoH and 2mol/l
Water purification to cumulative volume is that 30L adds again calcium carbonate 300g toward in tank, closes charge door, starts electrical heating, sets sterilising temp 118
~ 121 DEG C, after sterilization time 30min, chuck cooling water valve is opened, when making temperature in tank be down to 37 ~ 38 DEG C, opens inoculation mouth,
Alcohol pyrosphere is lighted, preprepared three-level round-bottomed flask probiotics fermention seed liquor 3000ml is inoculated with（Inoculum concentration is 10%）
It is rapid to close inoculation mouth, setting fermentation beginning and ending time, 37 ~ 38 DEG C of cultivation and fermentation temperature, fermentation time 18 ~ 20 hours, zymotic fluid
During pH value 3.8 ~ 4.2, zymotic fluid color is observed from depth to shallow, smell micro-perfume, that is, proceeding to spray drying unit carries out spray operation
Program, sets 160 ~ 180 DEG C of EAT, and 65 ~ 75 DEG C of leaving air temp is collected and is spray-dried, and dry product sealing is stored refrigerated, supplies
Detection and the filling capsule of compressed tablets are standby.
The quality measurements that product is produced to above example are as shown in Figure 1
The practical effect of this product is as shown in Figure 2.
The present invention comes from the herbaceous plant of integration of drinking and medicinal herbs, the unique nutrition of the bark of eucommia and bioactive ingredients, by screening benefit
Lactobacillus acidophilus and intestines streptococcus in raw bacterium member, in addition scientific formula and deep drainpipe fermentation, both have complementary advantages and double
To the result of fermentation, pure natural nutrient content and bioactive ingredients in the bark of eucommia, the need of growth of probiotics breeding had both been met
Ask, process of its fermentation further degrades bark of eucommia idiotrophic and bioactive ingredients so that the strong stomach of probio and
The health care of bark of eucommia strengthening the bones and muscles, obtains further lifting and embodies the natural dietotherapy theory of recurrence of radicallying reform.
Menarche Clinical practice, especially to patients such as person in middle and old age's gonarthromeningitis, osteoporosis and osteoarthrosis
Eating effect, achieve more satisfactory effect.
Claims (7)
1. a kind of health food and preparation method thereof, it is characterised in that：It adopts Bark of Eucommia Ulmoides and folium cortex eucommiae with 1:2 weight ratio enters
Row extracts concentration, then adds the nutriments such as peptone, beef extract, glucose, sodium chloride and makes corresponding nutrient solution, in training
Probio is inoculated with nutrient solution to be fermented, and finally terminal nutrient solution is dried prepared.
2. a kind of health food and preparation method thereof, it is characterised in that：
It includes following technological process：
（1）The preparation of culture medium：One-level, the preparation of secondary medium, three-level, the preparation of level Four culture medium；
（2）Probio is inoculated with；
（3）It is dried.
3. a kind of health food according to claim 2 and preparation method thereof, it is characterised in that：
The preparation process of culture medium is as follows：
（1）The preparation of one-level, secondary medium
Raw material：Peptone 1-4%, beef extract 0.1-0.5%, glucose 1-4%, sodium chloride 0.1-0.5%, eucommia leaf extract 1-
4%th, calcium carbonate 1-2%, pure water 86-95.8%；
Above proportioning is weighed according to proportion by weight；
Described eucommia leaf extract be by weight ratio be 1:Jing pure water is extracted after 1 folium cortex eucommiae and pure water infiltration, concentration,
It is spray-dried gained；
Step：By above-mentioned formula precise, and sequentially add plus heat container in, add part metering pure water, heating makes
Material all dissolves, and with the HCI of 20%NaoH and 2mol/l pH value 6.5-6.8 is adjusted, and finally supplies the pure water of surplus, stirs
Carefully dispensed after mixing uniformly, be placed in the sterilizing of electrothermal pressure sterilizer, controlled 115 DEG C -121 DEG C of sterilising temp, sterilization time
25-30min, after slant tube sterilizing, sets while hot in time inclined-plane, and sterilizing material is all down to after room temperature and turns refrigerator cold-storage, supplies
Inoculation and transferred species are standby；
（2）The preparation of three-level, level Four culture medium
Raw material：Folium cortex eucommiae, Bark of Eucommia Ulmoides, peptone, beef extract, glucose, sodium chloride, calcium carbonate, pure water；
Step：
1）The pretreatment of raw material, Bark of Eucommia Ulmoides is carried out processing the fragment for being broken into 1-2 centimetre of size；And pluck in annual drop frost
Fresh folium cortex eucommiae Jing instantaneous high-temperatures or the sun before is exposed to the sun, complete dezymotize pack after acceptance(check) it is standby；
2）Bark of Eucommia Ulmoides of weighing respectively is a, and two parts of folium cortex eucommiae is placed in two rustless steel containers respectively by 1：1 ratio, adds pure
Water purification is infiltrated, and Bark of Eucommia Ulmoides infiltrates 8 hours, and folium cortex eucommiae infiltrates 2 hours；
3）Extract：The folium cortex eucommiae for having infiltrated is transferred to the extractor extracted in thickener unit, then by 1：6 and 1：4 ratio
Plus pure water is extracted twice, for the first time 70 DEG C -80 DEG C of Extracting temperature of setting, 1 hour extraction time, arrange extract for the second time
Temperature is 70 DEG C -80 DEG C, 1 hour extraction time；
The Bark of Eucommia Ulmoides for having infiltrated is transferred into extractor, by 1:4 and 1:3 ratio adds pure water to carry out heating extraction twice；
For the first time 98 DEG C -100 DEG C of Extracting temperature of setting, 1 hour extraction time, second 98 DEG C -100 DEG C of Extracting temperature of setting, extract
1 hour time, merge extract twice and uniformly mix with bark of eucommia leaf extract；
4）Above-mentioned mixed uniformly extract is transferred into concentration by the filter divided into extraction thickener unit to fill, is started
Vacuum-concentrcted device, sets 65 DEG C -75 DEG C of thickening temperature, vacuum 0.06-0.08MPa, concentrate specific gravity control 1.10-
1.12；
5）Folium cortex eucommiae and Bark of Eucommia Ulmoides extract concentrate, the formula and preparation process of culture medium：Collect above-mentioned proportion 1.10-1.12's
Extract, accurate overall stoichiometric product, is placed in stainless steel plus heat container, sequentially adds peptone 1-2%, beef extract 0.1-0.2%, chlorine
Change sodium 0.1-0.2%, glucose 1-2%, treat peptone, beef extract, sodium chloride, glucose is all after dissolving, with 20%NaoH and
2mo1/1Hcl adjusts pH value 6.5-6.8, is eventually adding the calcium carbonate of 0.5-1.0%, and sterilising temp 115 ~ 121 is set after sealing
DEG C, 28 ~ 30min of sterilization time is cooled to 37 DEG C ± 2 DEG C after sterilizing.
4. a kind of health food according to claim 2 and preparation method thereof, it is characterised in that：Described probio is thermophilic
Lactobacillus lactis or intestines streptococcus
A kind of health products according to claim 4 and preparation method thereof, it is characterised in that：The inoculation method of probio is：
Bacterial classification is gone into first cell culture medium from slant strains, and go to successively two grades, three-level, level Four culture medium with expanding production, it is described
First cell culture medium and the cultivation temperature of secondary medium be 37 DEG C ± 2 DEG C, incubation time is 22-24 hours；Described
The cultivation temperature of three-stage culture medium and level Four culture medium is 37 DEG C -39 DEG C, and incubation time is 18-20 hours；When in culture medium
When zymotic fluid terminal PH is 3.8 ~ 4.2, you can zymotic fluid is transferred into next link.
5. a kind of health food according to claim 2 and preparation method thereof, it is characterised in that：Described dry run is
It is spray-dried and freeze-drying.
6. a kind of health products according to claim 5 and preparation method thereof, it is characterised in that：Described spray drying be by
The peristaltic pump of the zymotic fluid connection dryer group for fermenting, 160 ~ 180 DEG C of EAT of setting, 65 ~ 75 DEG C of leaving air temp, in time
Dry powder is collected, the filling capsule of stored refrigerated confession quality inspection compressing tablet is sealed standby.
7. a kind of health food according to claim 5 and preparation method thereof, it is characterised in that：Described freeze-drying is
By the zymotic fluid for fermenting, in environment purification, lyophilized plate is dispensed, pre-freeze 120 minutes at the temperature started shooting below -30 DEG C,
Then once distilled, defrost discharging after parsing-desiccation seals the filling capsule of stored refrigerated confession quality inspection compressing tablet standby.
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| CN107252111A (en) * | 2017-05-24 | 2017-10-17 | 灵宝市帅华牧业有限责任公司 | It is a kind of to aid in health food of diabetic's rehabilitation and preparation method thereof |
| CN110810684A (en) * | 2018-08-07 | 2020-02-21 | 三门峡金测质量技术检验检测有限责任公司 | Beverage prepared by fermenting folium Eucommiae and fructus Lycii with lactobacillus and its preparation method |
| CN112155214A (en) * | 2020-10-15 | 2021-01-01 | 三门峡梅奥生物工程有限公司 | Human nutrition granules produced by eucommia ulmoides liquid fermented by probiotics |
| CN112754011A (en) * | 2021-01-26 | 2021-05-07 | 昆明生物制造研究院有限公司 | Preparation method of walnut kernel skin health-care buccal tablets |
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Application publication date: 20170510 |