CN106615831A - Preparation method for producing fermented feed complex bacterial agent for pigs from vegetable waste and straw - Google Patents
Preparation method for producing fermented feed complex bacterial agent for pigs from vegetable waste and straw Download PDFInfo
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Abstract
The invention relates to a preparation method for producing a fermented feed complex bacterial agent for pigs from vegetable waste and straw. The method comprises steps as follows: (1) a solution A is obtained by evenly mixing milk and brown sugar in proportion; (2) a mixed bacterial solution is firstly added to the solution A, 1 part of inorganic salt is added to 1 L of the solution A, and a solution B is obtained after evenly mixing; (3) corn flour is added to the solution B and evenly stirred, and a raw material with the moisture content being 30%-45% is obtained; (4) the raw material is placed in a container, the container is sealed with a breathable film, fermentation is performed at 28-36 DEG C for 1-2 d, when the pH value is smaller than or equal to 4.5 and 2-20 billons cfu/g mixed bacteria exist, drying, crushing and bagging are performed under the sterile condition, and the fermented feed complex bacterial agent for the pigs is prepared. The process is simple, the cost is low, and the prepared complex bacterial agent can improve the fermentation efficiency, improve the feed quality, reduce nutrition loss and prolong the storage time of fermented feed.
Description
Technical field
The present invention relates to pig feed field, more particularly, to waste dish and stalk produce the composite bacteria agent of pig fermented feed
Preparation method.
Background technology
In recent years, Gansu Development of Vegetable Industry is rapid, it has also become the whole nation " adjusting in western food east ", the five big commodity of " Bei Cainan fortune "
One of Vegetable Base and Northwest inland export vegetable emphasis producing region.However, with the fast development of vegetables industry, vegetables are being adopted
The a large amount of waste dish produced during receipts, marketing fresh and preliminary working（Waste dish is vegetable castoff, mainly baby cabbage, wild cabbage, flower coconut palm
The discarded root of the vegetables such as dish, celery, asparagus lettuce, stem, leaf）.The whole province's growing vegetables area reaches 8,000,000 mu within 2016, and waste dish amount surpasses
Cross 11,000,000 tons.China is large agricultural country, and substantial amounts of agricultural byproducts do not make full use of, and causes and greatly waste and environment dirt
Dye, only agricultural crop straw are annual up to 5.7 hundred million tons.China is again pig big country, and with pig industry fast development, feed resource
Shortage phenomenon is more highlighted, and has become the bottleneck of restriction pig industry development.
The patent of Publication No. CN103518957A and CN105325689A, with waste dish and stalk as raw material, uses for reference ensiling
The ruminant ensilages such as the principle of technology, production cattle and sheep, have been carried out effectively in agriculture waste Resources as Animal Feedstuffs using aspect
Exploration.The present invention has not only widened pig feed source using waste dish and stalk production pig fermented feed, and has expanded agriculture
Industry waste resource fodder utilization ways, with wide market prospects and using value.At present, still lack using waste dish and agriculture
Crop material produces the mature technology of pig fermented feed, and special commodity microbial inoculum.
The content of the invention
The technical problem to be solved is to provide a kind of process is simple, with low cost gives birth to for waste dish and stalk
Produce the preparation method of the composite bacteria agent of pig fermented feed.
To solve the above problems, the composite bacteria agent for waste dish and stalk production pig fermented feed of the present invention
Preparation method, comprises the following steps：
(1) milk, brown sugar are pressed into 10kg ~ 20kg：1kg ratios are well mixed, that is, obtain solution A；
(2) mixed bacteria liquid, then 1 part of the inorganic salts of solution A addition as described in every 1L are initially charged in the solution A, after being well mixed,
Obtain solution B；The ratio of the mixed bacteria liquid and the solution A is 0.2kg ~ 0.4kg：10kg~20kg；
(3) corn flour is added in the solution B, stirred, obtain final product the raw material that moisture is 30% ~ 45%；The solution B
It is 1kg ~ 2kg with the ratio of the corn flour：9kg~12kg；
(4) the raw material is fitted into container, is sealed with ventilated membrane, in 28 DEG C ~ 36 DEG C condition bottom fermentations 1 ~ 2 day, when pH value≤
4.5th, when Mixed Microbes reach 20 ~ 20,000,000,000 cfu/g, it is dried under aseptic condition, crushes, packs, obtains final product pig fermented feed compound bacteria
Agent.
(1) middle milk refers to the skim milk after pasteurization to the step.
(2) 1 part of middle inorganic salts are by disodium citrate 2.0g, potassium dihydrogen phosphate 2.0g, dipotassium hydrogen phosphate to the step
2.0g, manganese sulfate 0.05g, calcium carbonate 20.0g compositions.
(2) middle mixed bacteria liquid is prepared as follows to the step：
1. respectively by bacterial classification candida utili（CICC 1314）, geotrichum candidum（CICC 1315）, saccharomyces cerevisiae（CICC1421）
Diluted with 10mL sterilized waters by 1g bacterial classifications, then take 1mL diluent As and be inoculated on wort agar culture medium, under the conditions of 37 DEG C
6 ~ 12h of culture, respectively obtains the Pure cultured spawn of three Yeasts；The malt agar slant medium is referred to 15g agar
It is dissolved in gained in 1L brewer's worts；
2. respectively by Lactobacillus plantarum（CICC6026）, animal bifidobacteria（CICC 21717）By 1g bacterial classifications 10mL sterilized waters
Dilution, then takes 1mL dilution B and is inoculated in MRS inclined-planes solid medium, and 6 ~ 12h is cultivated under the conditions of 37 DEG C, respectively obtains two kinds
The Pure cultured spawn of lactic acid bacteria；MRS inclined-planes solid medium is referred to casein peptone 10.0g, beef extract 10.0g, yeast
Powder 5.0g, glucose 5.0g, sodium acetate 5.0g, disodium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.05g, carbonic acid
Calcium 20.0g, agar 15g are dissolved in gained in 1L distilled water；
3. 1/2 ~ 2/3 solution A is filled in closed container, 5 ~ 10% inoculations described three of volume are filled by the solution A respectively
The Pure cultured spawn of Yeasts, is sealed with ventilated membrane, is culture on the shaking table of 180 ~ 220r/min in 28 DEG C ~ 36 DEG C, rotating speed
24h ~ 48h, when saccharomycete reaches 50,000,000,000 cfu/ml, respectively obtains the amplification bacterium solution of three Yeasts；
4. the Pure cultured spawn of the described two lactic acid bacterias of 5 ~ 10% inoculations of the solution A volume is pressed respectively, obtains final product two kinds of inoculations molten
Liquid, is separately filled with after two kinds of inoculation solution in closed container and seals, at 28 DEG C ~ 36 DEG C, quiescent culture 24h ~ 48h,
When lactic acid bacteria reaches 50,000,000,000 cfu/ml, the amplification bacterium solution of two kinds of lactic acid bacterias is respectively obtained；
5. the amplification bacterium solution of the amplification bacterium solution of three Yeasts and described two lactic acid bacterias is pressed into 1：1：1：1：1 weight ratio
After uniform mixing, mixed bacteria liquid is obtained final product.
(3) middle corn powder fineness is 80 ~ 100 mesh to the step.
The present invention has compared with prior art advantages below：
1st, it is of the invention with corn, milk, brown sugar as raw material, the pig that is produced on a large scale fermented feed composite bacteria agent, production technology
Simply, low raw-material cost.
2nd, present invention gained composite bacteria agent, can improve fermentation efficiency, lift feed quality, reduce nutritive loss, extend and send out
The ferment feed storage time.
3rd, using the fermented feed of present invention gained composite bacteria agent production, nutritious in yellowish-brown, aromatic flavour, acid
Comfortable mouth, can improve the feed intake of pig, and gaining effect is obvious.
4th, using the fermented feed of present invention gained composite bacteria agent production, rich in various probios, gut flora can be improved
Structure, reduces intestines problem and occurs, and strengthens the resistance of pig.
5th, using present invention gained composite bacteria agent, meet national feed addictive quality standard, produced using the microbial inoculum
Feed cost is cheap, is that breeding enterprise and raiser save feed cost, economic benefit, social benefit and obvious environment benefit.
Specific embodiment
Embodiment 1 is used for the preparation method of the composite bacteria agent of waste dish and stalk production pig fermented feed, including following step
Suddenly：
(1) milk, brown sugar are pressed into 10kg：1kg ratios are well mixed, that is, obtain solution A.
Wherein：Milk refers to the skim milk after pasteurization.
(2) 0.2kg mixed bacteria liquids are initially charged in 10kg solution As, then add 1 part of inorganic salts by every 1L solution As, mixing is equal
After even, that is, obtain solution B.
Wherein：1 part of inorganic salts are by disodium citrate 2.0g, potassium dihydrogen phosphate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate
0.05g, calcium carbonate 20.0g are constituted.
Mixed bacteria liquid is prepared as follows：
1. respectively by bacterial classification candida utili（CICC 1314）, geotrichum candidum（CICC 1315）, saccharomyces cerevisiae（CICC1421）
Diluted with 10mL sterilized waters by 1g bacterial classifications, then take 1mL diluent As and be inoculated on wort agar culture medium, under the conditions of 37 DEG C
6 ~ 12h of culture, respectively obtains the Pure cultured spawn of three Yeasts；Malt agar slant medium is referred to and is dissolved in 15g agar
Gained in 1L brewer's worts；
2. respectively by Lactobacillus plantarum（CICC6026）, animal bifidobacteria（CICC 21717）By 1g bacterial classifications 10mL sterilized waters
Dilution, then takes 1mL dilution B and is inoculated in MRS inclined-planes solid medium, and 6 ~ 12h is cultivated under the conditions of 37 DEG C, respectively obtains two kinds
The Pure cultured spawn of lactic acid bacteria；MRS inclined-planes solid medium is referred to casein peptone 10.0g, beef extract 10.0g, dusty yeast
5.0g, glucose 5.0g, sodium acetate 5.0g, disodium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.05g, calcium carbonate
20.0g, agar 15g are dissolved in gained in 1L distilled water；
3. 1/2 ~ 2/3 solution A is filled in closed container, 5 ~ 10% three Yeasts of inoculation of volume are filled by solution A respectively
Pure cultured spawn, with ventilated membrane seal, 28 DEG C ~ 36 DEG C, rotating speed for 180 ~ 220r/min shaking table on cultivate 24h ~ 48h,
When saccharomycete reaches 50,000,000,000 cfu/ml, the amplification bacterium solution of three Yeasts is respectively obtained；
4. the Pure cultured spawn of 5 ~ 10% inoculating two kinds lactic acid bacterias of solution A volume is pressed respectively, two kinds of inoculation solution is obtained final product, close
Close in container and be separately filled with after two kinds of inoculation solution and seal, at 28 DEG C ~ 36 DEG C, quiescent culture 24h ~ 48h, when lactic acid bacteria reaches
During 50000000000 cfu/ml, the amplification bacterium solution of two kinds of lactic acid bacterias is respectively obtained；
5. the amplification bacterium solution of the amplification bacterium solution of three Yeasts and two kinds of lactic acid bacterias is pressed into 1：1：1：1：1 weight is than uniform mixing
Afterwards, mixed bacteria liquid is obtained final product.
(3) add 9kg fineness for the corn flour of 80 ~ 100 mesh in 1kg solution Bs, stir, obtaining final product moisture is
30% ~ 45% raw material.
(4) raw material is fitted into container, is sealed with ventilated membrane, in 28 DEG C ~ 36 DEG C condition bottom fermentations 1 ~ 2 day, when pH value≤
4.5th, when Mixed Microbes reach 20 ~ 20,000,000,000 cfu/g, it is dried under aseptic condition, crushes, packs, obtains final product pig fermented feed compound bacteria
Agent.
Embodiment 2 is used for the preparation method of the composite bacteria agent of waste dish and stalk production pig fermented feed, including following step
Suddenly：
(1) milk, brown sugar are pressed into 20kg：1kg ratios are well mixed, that is, obtain solution A.
Wherein milk is with embodiment 1.
(2) 0.4kg mixed bacteria liquids are initially charged in 20kg solution As, then add 1 part of inorganic salts by every 1L solution As, mixing is equal
After even, that is, obtain solution B.
Wherein：1 part of inorganic salts, mixed bacteria liquid are with embodiment 1.
(3) add 12kg fineness for the corn flour of 80 ~ 100 mesh in 2kg solution Bs, stir, obtaining final product moisture is
30% ~ 45% raw material.
(4) raw material is fitted into container, is sealed with ventilated membrane, in 28 DEG C ~ 36 DEG C condition bottom fermentations 1 ~ 2 day, when pH value≤
4.5th, when Mixed Microbes reach 20 ~ 20,000,000,000 cfu/g, it is dried under aseptic condition, crushes, packs, obtains final product pig fermented feed compound bacteria
Agent.
Embodiment 3 is used for the preparation method of the composite bacteria agent of waste dish and stalk production pig fermented feed, including following step
Suddenly：
(1) milk, brown sugar are pressed into 15kg：1kg ratios are well mixed, that is, obtain solution A.
Wherein milk is with embodiment 1.
(2) 0.3kg mixed bacteria liquids are initially charged in 15kg solution As, then add 1 part of inorganic salts by every 1L solution As, mixing is equal
After even, that is, obtain solution B.
Wherein：1 part of inorganic salts, mixed bacteria liquid are with embodiment 1.
(3) add 10kg fineness for the corn flour of 80 ~ 100 mesh in 1.5kg solution Bs, stir, obtain final product moisture
For 30% ~ 45% raw material.
(4) raw material is fitted into container, is sealed with ventilated membrane, in 28 DEG C ~ 36 DEG C condition bottom fermentations 1 ~ 2 day, when pH value≤
4.5th, when Mixed Microbes reach 20 ~ 20,000,000,000 cfu/g, it is dried under aseptic condition, crushes, packs, obtains final product pig fermented feed compound bacteria
Agent.
Claims (5)
1. it is used for the preparation method of the composite bacteria agent of waste dish and stalk production pig fermented feed, comprises the following steps：
(1) milk, brown sugar are pressed into 10kg ~ 20kg：1kg ratios are well mixed, that is, obtain solution A；
(2) mixed bacteria liquid, then 1 part of the inorganic salts of solution A addition as described in every 1L are initially charged in the solution A, after being well mixed,
Obtain solution B；The ratio of the mixed bacteria liquid and the solution A is 0.2kg ~ 0.4kg：10kg~20kg；
(3) corn flour is added in the solution B, stirred, obtain final product the raw material that moisture is 30% ~ 45%；The solution B
It is 1kg ~ 2kg with the ratio of the corn flour：9kg~12kg；
(4) the raw material is fitted into container, is sealed with ventilated membrane, in 28 DEG C ~ 36 DEG C condition bottom fermentations 1 ~ 2 day, when pH value≤
4.5th, when Mixed Microbes reach 20 ~ 20,000,000,000 cfu/g, it is dried under aseptic condition, crushes, packs, obtains final product pig fermented feed compound bacteria
Agent.
2. the preparation method of the composite bacteria agent of waste dish and stalk production pig fermented feed is used for as claimed in claim 1, its
It is characterised by：(1) middle milk refers to the skim milk after pasteurization to the step.
3. the preparation method of the composite bacteria agent of waste dish and stalk production pig fermented feed is used for as claimed in claim 1, its
It is characterised by：(2) 1 part of middle inorganic salts are by disodium citrate 2.0g, potassium dihydrogen phosphate 2.0g, dipotassium hydrogen phosphate to the step
2.0g, manganese sulfate 0.05g, calcium carbonate 20.0g compositions.
4. the preparation method of the composite bacteria agent of waste dish and stalk production pig fermented feed is used for as claimed in claim 1, its
It is characterised by：(2) middle mixed bacteria liquid is prepared as follows to the step：
1. respectively bacterial classification candida utili, geotrichum candidum, saccharomyces cerevisiae are pressed into 1g bacterial classification 10mL sterilized waters to dilute, Ran Houqu
1mL diluent As are inoculated on wort agar culture medium, and 6 ~ 12h is cultivated under the conditions of 37 DEG C, respectively obtain the pure of three Yeasts
Culture bacterial classification；The malt agar slant medium is referred to and for 15g agar to be dissolved in gained in 1L brewer's worts；
2. respectively Lactobacillus plantarum, animal bifidobacteria are pressed into 1g bacterial classification 10mL sterilized waters to dilute, then takes 1mL dilution B
MRS inclined-planes solid medium is inoculated in, 6 ~ 12h is cultivated under the conditions of 37 DEG C, respectively obtain the Pure cultured spawn of two kinds of lactic acid bacterias；Institute
State MRS inclined-planes solid medium to refer to casein peptone 10.0g, beef extract 10.0g, dusty yeast 5.0g, glucose 5.0g, acetic acid
Sodium 5.0g, disodium citrate 2.0g, dipotassium hydrogen phosphate 2.0g, manganese sulfate 0.05g, calcium carbonate 20.0g, agar 15g are dissolved in 1L steamings
Gained in distilled water；
3. 1/2 ~ 2/3 solution A is filled in closed container, 5 ~ 10% inoculations described three of volume are filled by the solution A respectively
The Pure cultured spawn of Yeasts, is sealed with ventilated membrane, is culture on the shaking table of 180 ~ 220r/min in 28 DEG C ~ 36 DEG C, rotating speed
24h ~ 48h, when saccharomycete reaches 50,000,000,000 cfu/ml, respectively obtains the amplification bacterium solution of three Yeasts；
4. the Pure cultured spawn of the described two lactic acid bacterias of 5 ~ 10% inoculations of the solution A volume is pressed respectively, obtains final product two kinds of inoculations molten
Liquid, is separately filled with after two kinds of inoculation solution in closed container and seals, at 28 DEG C ~ 36 DEG C, quiescent culture 24h ~ 48h,
When lactic acid bacteria reaches 50,000,000,000 cfu/ml, the amplification bacterium solution of two kinds of lactic acid bacterias is respectively obtained；
5. the amplification bacterium solution of the amplification bacterium solution of three Yeasts and described two lactic acid bacterias is pressed into 1：1：1：1：1 weight ratio
After uniform mixing, mixed bacteria liquid is obtained final product.
5. the preparation method of the composite bacteria agent of waste dish and stalk production pig fermented feed is used for as claimed in claim 1, its
It is characterised by：(3) middle corn powder fineness is 80 ~ 100 mesh to the step.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109198166A (en) * | 2018-11-27 | 2019-01-15 | 甘肃省农业科学院农产品贮藏加工研究所 | A method of fermented feed is prepared using waste dish |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109198166A (en) * | 2018-11-27 | 2019-01-15 | 甘肃省农业科学院农产品贮藏加工研究所 | A method of fermented feed is prepared using waste dish |
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| CN106615831B (en) | 2020-10-02 |
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