CN102864104A - Preparation method and application of composite bacteria preparation for livestock and poultry fermented feed - Google Patents
Preparation method and application of composite bacteria preparation for livestock and poultry fermented feed Download PDFInfo
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- CN102864104A CN102864104A CN2012103327976A CN201210332797A CN102864104A CN 102864104 A CN102864104 A CN 102864104A CN 2012103327976 A CN2012103327976 A CN 2012103327976A CN 201210332797 A CN201210332797 A CN 201210332797A CN 102864104 A CN102864104 A CN 102864104A
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Abstract
The invention discloses a preparation method and application of a composite bacteria preparation for a livestock and poultry fermented feed. Selected lactic acid bacteria are anaerobes which are subjected to homolactic fermentation, mainly comprise enterococcus faecium, enterococcus faecalis and streptococcus thermophiles, are the most common lactic acid bacteria in the livestock and poultry industry; no gas is substantially produced in a metabolism process; a fermentation system is only required to keep better tightness; and the composite bacteria preparation has better applicability for fermentation of most of livestock and poultry feeds. Selected yeast is bread yeast, i.e., the yeast which is circulated on the commercial market for producing bread; and every gram of bread yeast dry powder contains 100 hundred millions of cfu active yeasts; the water content of the prepared composite bacteria preparation generally does not exceed 9.0 percent; the composite bacteria preparation can be stored for 3-6 months under a vacuum package condition; the number of the yeasts is kept basically unchanged; and the number of the active lactic acid bacteria is kept at about 50-65 percent. The fermentation can be excellently started by inoculating 0.1-0.2 percent of composite bacteria product in the livestock and poultry fermented feed.
Description
Technical field
The present invention relates to a kind of composite bacteria, particularly a kind of livestock and poultry fermented feed is used the preparation method of composite bacteria preparation and is used.
Background technology
Fermented feed refers under the manual control condition, microorganism is by the Metabolic activity of self, antinutritional factor in vegetalitas, animality and the mineral material is decomposed or transform, producing more can be by the search for food feedstuff raw material of the higher and toxicological harmless effect of the nutrient of digestion, absorption of livestock.The microorganism that produces the livestock and poultry fermented feed mainly has yeast and milk-acid bacteria, sometimes also has a small amount of genus bacillus.But for common animal and fowl fodder, if water content reaches more than 30%, there are an amount of yeast and milk-acid bacteria just enough to start and fermented.At present, the livestock and poultry cultivation industry begins to promote the use of fermented feed gradually, but the bacterial classification of producing fermented feed all is that production technique more complicated, price are also very high by biotech company's production of specialty.In fact, the bacterial classification preparation that production livestock and poultry fermented feed is used does not need very complicated operating procedure, does not need very large equipment investment yet, and some easy production technologies can reach very desirable effect equally.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of livestock and poultry fermented feed preparation method and the application thereof of composite bacteria preparation, the operation of preparation composite bacteria preparation is few, technique is simple, and is easy to operate, need not the input of main equipment, generalization is strong, saves Financial cost; Prepared composite bacteria preparation can effectively promote the conversion of feed nutrient, improves food conversion ratio.
The present invention discloses the preparation method that a kind of livestock and poultry fermented feed is used composite bacteria preparation, described composite bacteria preparation is the composite bacteria preparation that contains bread microzyme and milk-acid bacteria, the anaerobism milk-acid bacteria of described milk-acid bacteria for adopting the high density liquid anaerobism to cultivate, the preparation method of described composite bacteria preparation may further comprise the steps:
A. prepare liquid lactic acid bacterium culture medium to be inoculated: brown sugar and milk are dissolved also sealed storage fully by weight mix to brown sugar for brown sugar: milk=1:19-22, and temperature be under 105-115 ℃ the sterilization 18-22 minute, then be cooled to normal temperature and be liquid lactic acid bacterium culture medium to be inoculated;
B. the inoculum for preparing liquid lactic acid bacterium culture medium: doubly to milk wt water doubly milk is diluted with 1-3, then adding weight ratio is sucrose: be stirred to sucrose after the sucrose of milk diluent=1:25-30 and dissolve fully, this mixed solution after being 115-125 ℃ of lower the sterilization 18-22 minute, temperature is cooled to normal temperature, then add the milk-acid bacteria that weight ratio is mixed solution: milk-acid bacteria=50:0.05-0.1, and be 35-40 ℃ of lower constant temperature culture 22-26 hour in temperature, namely get inoculum;
C. with sealed storage behind the inoculum among the liquid lactic acid bacterium culture medium among the step a access step b, be that 35-45 ℃ of lower constant temperature tranquillization is cultured to substratum and occurs significantly solidifying shape in temperature, namely get lactic acid bacteria culture solution;
D. the kiln malt powder that the lactic acid bacteria culture solution among the step c is added 30-40 lactic acid bacteria culture solution times of weight adsorbed 3-7 minute, then add bread yeast powder mix and blend 1-4 minute of lactic acid bacteria culture solution times of weight after the 1-2 absorption, make and produce livestock and poultry fermented feed composite bacteria preparation;
Further, among the step a, brown sugar dissolves fully and sealed storage by weight mixing to for brown sugar: milk=1:20 with brown sugar and milk, and is sterilization 20 minutes under 110 ℃ in temperature, then is cooled to normal temperature and is liquid lactic acid bacterium culture medium to be inoculated;
Further, among the step a, described milk is not for containing the milk of microbiotic and not diluted;
Further, among the step b, milk is diluted to milk wt water doubly with 2 times, then adding weight ratio is sucrose: be stirred to sucrose after the sucrose of milk diluent=1:28 and dissolve fully, this mixed solution after being 120 ℃ of lower sterilizations 20 minutes, temperature is cooled to normal temperature, then add the milk-acid bacteria that weight ratio is mixed solution: milk-acid bacteria=50:0.08, and be 37 ℃ of lower constant temperature culture 24 hours in temperature, namely get inoculum;
Further, among the step c, with sealed storage behind the inoculum among the liquid lactic acid bacterium culture medium among the step a access step b, be that 40 ℃ of lower constant temperature tranquillization are cultured to substratum and occur significantly solidifying shape in temperature, namely get lactic acid bacteria culture solution;
Further, in the steps d, lactic acid bacteria culture solution among the step c is added the kiln malt powder absorption 5 minutes of 35 lactic acid bacteria culture solution times of weight, then add the bread yeast powder mix and blend 3 minutes of lactic acid bacteria culture solution times of weight after 1.5 absorption, make and produce livestock and poultry fermented feed composite bacteria preparation;
Further, described anaerobism milk-acid bacteria comprises one or more mixtures in thermophilus streptococcus (Streptococcus thermophilus), enterococcus faecalis (Enterococcus.faecalis) and the faecium (Enterococcus.faecium).
Further, described bread microzyme adopts every gram bread yeast dry powder to contain the active yeast of 10,000,000,000 cfu;
The present invention also discloses a kind of livestock and poultry fermented feed with the application of composite bacteria preparation, and adding weight percent in the feedstuff raw material to be fermented is the livestock and poultry fermented feed composite bacteria preparation of 0.1-0.2%.
Beneficial effect of the present invention: livestock and poultry fermented feed of the present invention is used the preparation method of composite bacteria preparation and is used, the lactic acid fermented anerobe of milk-acid bacteria homotype of selecting, these milk-acid bacterias mainly comprise faecium, enterococcus faecalis and thermophilus streptococcus, they all are the most frequently used milk-acid bacterias of livestock and poultry breeding industry, metabolic process does not produce gas substantially, as long as keep fermentation system that preferably stopping property is arranged, these milk-acid bacterias just can survive and metabolism well, and the fermentation of most animal and fowl fodders is had preferably suitability.The yeast of selecting is bread microzyme, is exactly the yeast of the production bread that circulates on the commercial market, and every gram bread yeast dry powder contains 10,000,000,000 cfu active yeasts; This yeast juice has the fine very widely scope of application, is the good microorganism of a kind of production livestock and poultry fermented feed.Particularly at the initial period of fermentation, this Yeasts has very strong fermenting power, can consume rapidly oxygen residual in the raw material, for Fast-propagation and the eubolism of milk-acid bacteria provides good anaerobic environment.Bread microzyme is to adopt ripe bread yeast dry powder (Angel Yeast powder, food grade bread yeast, commercialization circulation).Milk-acid bacteria is adopted the liquid cultivation of high density, and the malt meal with drying after cultivation finishes adsorbs, and the ratio of absorption is 30～40.Namely the malt meal of 30～40 times of weight mixes with the liquid lactic acid bacteria culture fluid of 1 times of weight.Because the water content of common kiln malt powder generally all is no more than 5%, therefore by after the vast scale absorption dilution, the water content in the finished product generally is no more than 9.0%.Can deposit under vacuum-packed condition 3-6 month, saccharomycetic quantity is substantially constant, and the quantity of living lactic acid bacteria is kept at about 50-65%.This composite bacteria product inoculates 0.1～0.2% in the livestock and poultry fermented feed just can start fermentation well, is applicable to simultaneously aerobic fermentation and anaerobically fermenting, more suitable for the anaerobically fermenting system.
Embodiment
Embodiment one
A. prepare liquid lactic acid bacterium culture medium to be inoculated: with brown sugar and not diluted and do not contain antibiotic milk by weight mixing to brown sugar and dissolve fully for brown sugar: milk=1:20, then be divided in the triangular flask, bottleneck seals with tampon, outsourcing kraft paper sealed storage, and temperature be under 110 ℃ the sterilization 20 minutes, then be cooled to normal temperature and be liquid lactic acid bacterium culture medium to be inoculated; Brown sugar also can have cane sugar substitution, can reach experiment purpose equally.
B. the inoculum for preparing liquid lactic acid bacterium culture medium: to antibiotic-free milk wt water doubly milk is diluted with 2 times, then adding weight ratio is sucrose: be stirred to sucrose after the sucrose of milk diluent=1:28 and dissolve fully, solution is contained in the vial, with the airtight glass bottle opening of rubber plug, this mixed solution is cooled to normal temperature after temperature is 120 ℃ of lower sterilizations 20 minutes transfer to later on the Bechtop, then opening bottle closure of rubber adding weight ratio is mixed solution: the anaerobism milk-acid bacteria of anaerobism milk-acid bacteria=50:0.08 is also used the airtight bottleneck of rubber plug, transfer in the constant incubator, and be 37 ℃ of lower constant temperature culture 24 hours in temperature, namely get inoculum; If the ripe untimely usefulness of kind liquid need to be kept in 4 ℃ of refrigerators, validity period is 10 days.
C. with sealed storage behind the inoculum among the liquid lactic acid bacterium culture medium among the step a access step b, be that 40 ℃ of lower constant temperature tranquillization are cultured to substratum and occur significantly solidifying shape in temperature, namely get lactic acid bacteria culture solution;
D. the kiln malt powder that the lactic acid bacteria culture solution among the step c is added 35 lactic acid bacteria culture solution times of weight adsorbed 5 minutes, namely the malt meal of 35 times of weight mixes with the lactic acid bacteria culture solution of 1 times of weight, then the bread yeast powder mix and blend 3 minutes that adds lactic acid bacteria culture solution times of weight after 1.5 absorption, namely the lactic acid bacteria culture solution of the bread yeast powder of 1.5 times of weight and 1 times of weight (after the absorption) mixes, bread microzyme adopts every gram bread yeast dry powder to contain the active yeast of 10,000,000,000 cfu, make livestock and poultry fermented feed composite bacteria preparation, composite bacteria preparation is carried out vacuum bagging preserve.
The application of composite bacteria preparation of the livestock and poultry fermented feed of present embodiment, add weight percent in the feedstuff raw material to be fermented and be 0.15% livestock and poultry fermented feed composite bacteria preparation, just can start well fermentation, be applicable to simultaneously aerobic fermentation and anaerobically fermenting, more suitable for the anaerobically fermenting system.
In the present embodiment, described anaerobism milk-acid bacteria is thermophilus streptococcus; In the present embodiment, with thermophilus streptococcus by equivalent weight than replacing with a kind of in enterococcus faecalis, the faecium, perhaps according to equivalent weight than the mixture that replaces with in thermophilus streptococcus, enterococcus faecalis, the faecium, all obtain qualified livestock and poultry fermented feed composite bacteria preparation, products obtained therefrom character there is no significant difference.
The livestock and poultry fermented feed composite bacteria preparation that present embodiment makes, water content is 0.6%, deposits 6 months under vacuum-packed condition, and saccharomycetic quantity is substantially constant, and the quantity of living lactic acid bacteria is kept at 65%.
Embodiment two
A. prepare liquid lactic acid bacterium culture medium to be inoculated: with brown sugar and not diluted and do not contain antibiotic milk by weight mixing to brown sugar and dissolve fully for brown sugar: milk=1:19, then be divided in the triangular flask, bottleneck seals with tampon, outsourcing kraft paper sealed storage, and temperature be under 105 ℃ the sterilization 18 minutes, then be cooled to normal temperature and be liquid lactic acid bacterium culture medium to be inoculated; Brown sugar also can have cane sugar substitution, can reach experiment purpose equally.
B. the inoculum for preparing liquid lactic acid bacterium culture medium: to antibiotic-free milk wt water doubly milk is diluted with 1 times, then adding weight ratio is sucrose: be stirred to sucrose after the sucrose of milk diluent=1:25 and dissolve fully, solution is contained in the vial, with the airtight glass bottle opening of rubber plug, this mixed solution is cooled to normal temperature after temperature is 115 ℃ of lower sterilizations 18 minutes transfer to later on the Bechtop, then opening bottle closure of rubber adding weight ratio is mixed solution: the anaerobism milk-acid bacteria of anaerobism milk-acid bacteria=50:0.05 is also used the airtight bottleneck of rubber plug, transfer in the constant incubator, and be 35 ℃ of lower constant temperature culture 22 hours in temperature, namely get inoculum; If the ripe untimely usefulness of kind liquid need to be kept in 4 ℃ of refrigerators, validity period is 10 days.
C. (rear sealed storage is that 35 ℃ of lower constant temperature tranquillization are cultured to substratum and occur significantly solidifying shape in temperature, namely gets lactic acid bacteria culture solution with the inoculum among the liquid lactic acid bacterium culture medium among the step a access step b;
D. the kiln malt powder that the lactic acid bacteria culture solution among the step c is added 30 lactic acid bacteria culture solution times of weight adsorbed 3 minutes, namely the malt meal of 30 times of weight mixes with the lactic acid bacteria culture solution of 1 times of weight, then the bread yeast powder mix and blend 1 minute that adds lactic acid bacteria culture solution times of weight after 1 absorption, namely the lactic acid bacteria culture solution of the bread yeast powder of 1 times of weight and 1 times of weight (after the absorption) mixes, bread microzyme adopts every gram bread yeast dry powder to contain the active yeast of 10,000,000,000 cfu, make livestock and poultry fermented feed composite bacteria preparation, composite bacteria preparation is carried out vacuum bagging preserve.
The application of composite bacteria preparation of the livestock and poultry fermented feed of present embodiment, add weight percent in the feedstuff raw material to be fermented and be 0.1% livestock and poultry fermented feed composite bacteria preparation, just can start well fermentation, be applicable to simultaneously aerobic fermentation and anaerobically fermenting, more suitable for the anaerobically fermenting system.
In the present embodiment, described anaerobism milk-acid bacteria is enterococcus faecalis; In the present embodiment, with enterococcus faecalis by equivalent weight than replacing with a kind of in thermophilus streptococcus, the faecium, perhaps according to equivalent weight than the mixture that replaces with in thermophilus streptococcus, enterococcus faecalis, the faecium, all obtain qualified livestock and poultry fermented feed composite bacteria preparation, products obtained therefrom character there is no significant difference.
The livestock and poultry fermented feed composite bacteria preparation that present embodiment makes, water content is 0.8%, deposits 3 months under vacuum-packed condition, and saccharomycetic quantity is substantially constant, and the quantity of living lactic acid bacteria is kept at 50%.
Embodiment three
A. prepare liquid lactic acid bacterium culture medium to be inoculated: with brown sugar and not diluted and do not contain antibiotic milk by weight mixing to brown sugar and dissolve fully for brown sugar: milk=1:22, then be divided in the triangular flask, bottleneck seals with tampon, outsourcing kraft paper sealed storage, and temperature be under 115 ℃ the sterilization 22 minutes, then be cooled to normal temperature and be liquid lactic acid bacterium culture medium to be inoculated; Brown sugar also can have cane sugar substitution, can reach experiment purpose equally.
B. the inoculum for preparing liquid lactic acid bacterium culture medium: to antibiotic-free milk wt water doubly milk is diluted with 3 times, then adding weight ratio is sucrose: be stirred to sucrose after the sucrose of milk diluent=1:30 and dissolve fully, solution is contained in the vial, with the airtight glass bottle opening of rubber plug, this mixed solution is cooled to normal temperature after temperature is 125 ℃ of lower sterilizations 22 minutes transfer to later on the Bechtop, then opening bottle closure of rubber adding weight ratio is mixed solution: the anaerobism milk-acid bacteria of anaerobism milk-acid bacteria=50:0.1 is also used the airtight bottleneck of rubber plug, transfer in the constant incubator, and be 40 ℃ of lower constant temperature culture 26 hours in temperature, namely get inoculum; If the ripe untimely usefulness of kind liquid need to be kept in 4 ℃ of refrigerators, validity period is 10 days.
C. with sealed storage behind the inoculum among the liquid lactic acid bacterium culture medium among the step a access step b, be that 35 ℃ of lower constant temperature tranquillization are cultured to substratum and occur significantly solidifying shape in temperature, namely get lactic acid bacteria culture solution;
D. the kiln malt powder that the lactic acid bacteria culture solution among the step c is added 30 lactic acid bacteria culture solution times of weight adsorbed 3 minutes, namely the malt meal of 30 times of weight mixes with the lactic acid bacteria culture solution of 1 times of weight, then the bread yeast powder mix and blend 1 minute that adds lactic acid bacteria culture solution times of weight after 1 absorption, namely the lactic acid bacteria culture solution of the bread yeast powder of 1 times of weight and 1 times of weight (after the absorption) mixes, bread microzyme adopts every gram bread yeast dry powder to contain the active yeast of 10,000,000,000 cfu, make livestock and poultry fermented feed composite bacteria preparation, composite bacteria preparation is carried out vacuum bagging preserve.
The application of composite bacteria preparation of the livestock and poultry fermented feed of present embodiment, add weight percent in the feedstuff raw material to be fermented and be 0.1% livestock and poultry fermented feed composite bacteria preparation, just can start well fermentation, be applicable to simultaneously aerobic fermentation and anaerobically fermenting, more suitable for the anaerobically fermenting system.
In the present embodiment, described anaerobism milk-acid bacteria is faecium; In the present embodiment, with faecium by equivalent weight than replacing with a kind of in thermophilus streptococcus, the enterococcus faecalis, perhaps according to equivalent weight than the mixture that replaces with in thermophilus streptococcus, enterococcus faecalis, the faecium, all obtain qualified livestock and poultry fermented feed composite bacteria preparation, products obtained therefrom character there is no significant difference.
The livestock and poultry fermented feed composite bacteria preparation that present embodiment makes, water content is 0.8%, deposits 3 months under vacuum-packed condition, and saccharomycetic quantity is substantially constant, and the quantity of living lactic acid bacteria is kept at 50%.
Embodiment four
A. prepare liquid lactic acid bacterium culture medium to be inoculated: with brown sugar and not diluted and do not contain antibiotic milk by weight mixing to brown sugar and dissolve fully for brown sugar: milk=1:22, then be divided in the triangular flask, bottleneck seals with tampon, outsourcing kraft paper sealed storage, and temperature be under 105 ℃ the sterilization 22 minutes, then be cooled to normal temperature and be liquid lactic acid bacterium culture medium to be inoculated; Brown sugar also can have cane sugar substitution, can reach experiment purpose equally.
B. the inoculum for preparing liquid lactic acid bacterium culture medium: to antibiotic-free milk wt water doubly milk is diluted with 3 times, then adding weight ratio is sucrose: be stirred to sucrose after the sucrose of milk diluent=1:30 and dissolve fully, solution is contained in the vial, with the airtight glass bottle opening of rubber plug, this mixed solution is cooled to normal temperature after temperature is 115 ℃ of lower sterilizations 22 minutes transfer to later on the Bechtop, then opening bottle closure of rubber adding weight ratio is mixed solution: the anaerobism milk-acid bacteria of anaerobism milk-acid bacteria=50:0.1 is also used the airtight bottleneck of rubber plug, transfer in the constant incubator, and be 40 ℃ of lower constant temperature culture 22 hours in temperature, namely get inoculum; If the ripe untimely usefulness of kind liquid need to be kept in 4 ℃ of refrigerators, validity period is 10 days.
C. with sealed storage behind the inoculum among the liquid lactic acid bacterium culture medium among the step a access step b, be that 35 ℃ of lower constant temperature tranquillization are cultured to substratum and occur significantly solidifying shape in temperature, namely get lactic acid bacteria culture solution;
D. the kiln malt powder that the lactic acid bacteria culture solution among the step c is added 40 lactic acid bacteria culture solution times of weight adsorbed 3 minutes, namely the malt meal of 40 times of weight mixes with the lactic acid bacteria culture solution of 1 times of weight, then the bread yeast powder mixing that adds the rear lactic acid bacteria culture solution times of weight of 2 absorption was stirred 7 minutes, namely the lactic acid bacteria culture solution of the bread yeast powder of 2 times of weight and 1 times of weight (after the absorption) mixes, bread microzyme adopts every gram bread yeast dry powder to contain the active yeast of 10,000,000,000 cfu, make livestock and poultry fermented feed composite bacteria preparation, composite bacteria preparation is carried out vacuum bagging preserve.
The application of composite bacteria preparation of the livestock and poultry fermented feed of present embodiment, add weight percent in the feedstuff raw material to be fermented and be 0.15% livestock and poultry fermented feed composite bacteria preparation, just can start well fermentation, be applicable to simultaneously aerobic fermentation and anaerobically fermenting, more suitable for the anaerobically fermenting system.
In the present embodiment, described anaerobism milk-acid bacteria is thermophilus streptococcus; In the present embodiment, with thermophilus streptococcus by equivalent weight than replacing with a kind of in enterococcus faecalis, the faecium, perhaps according to equivalent weight than the mixture that replaces with in thermophilus streptococcus, enterococcus faecalis, the faecium, all obtain qualified livestock and poultry fermented feed composite bacteria preparation, products obtained therefrom character there is no significant difference.
The livestock and poultry fermented feed composite bacteria preparation that present embodiment makes, water content is 0.7%, deposits 5 months under vacuum-packed condition, and saccharomycetic quantity is substantially constant, and the quantity of living lactic acid bacteria is kept at 60%.
Embodiment five
A. prepare liquid lactic acid bacterium culture medium to be inoculated: with brown sugar and not diluted and do not contain antibiotic milk by weight mixing to brown sugar and dissolve fully for brown sugar: milk=1:19, then be divided in the triangular flask, bottleneck seals with tampon, outsourcing kraft paper sealed storage, and temperature be under 112 ℃ the sterilization 19 minutes, then be cooled to normal temperature and be liquid lactic acid bacterium culture medium to be inoculated; Brown sugar also can have cane sugar substitution, can reach experiment purpose equally.
B. the inoculum for preparing liquid lactic acid bacterium culture medium: to antibiotic-free milk wt water doubly milk is diluted with 2 times, then adding weight ratio is sucrose: be stirred to sucrose after the sucrose of milk diluent=1:27 and dissolve fully, solution is contained in the vial, with the airtight glass bottle opening of rubber plug, this mixed solution is cooled to normal temperature after temperature is 122 ℃ of lower sterilizations 19 minutes transfer to later on the Bechtop, then opening bottle closure of rubber adding weight ratio is mixed solution: the anaerobism milk-acid bacteria of anaerobism milk-acid bacteria=50:0.06 is also used the airtight bottleneck of rubber plug, transfer in the constant incubator, and be 37 ℃ of lower constant temperature culture 25 hours in temperature, namely get inoculum; If the ripe untimely usefulness of kind liquid need to be kept in 4 ℃ of refrigerators, validity period is 10 days.
C. with sealed storage behind the inoculum among the liquid lactic acid bacterium culture medium among the step a access step b, be that 42 ℃ of lower constant temperature tranquillization are cultured to substratum and occur significantly solidifying shape in temperature, namely get lactic acid bacteria culture solution;
D. the kiln malt powder that the lactic acid bacteria culture solution among the step c is added 32 lactic acid bacteria culture solution times of weight adsorbed 3 minutes, namely the malt meal of 32 times of weight mixes with the lactic acid bacteria culture solution of 1 times of weight, then the bread yeast powder mix and blend 1 minute that adds lactic acid bacteria culture solution times of weight after 1.5 absorption, namely the lactic acid bacteria culture solution of the bread yeast powder of 1.5 times of weight and 1 times of weight (after the absorption) mixes, bread microzyme adopts every gram bread yeast dry powder to contain the active yeast of 10,000,000,000 cfu, make livestock and poultry fermented feed composite bacteria preparation, composite bacteria preparation is carried out vacuum bagging preserve.
The application of composite bacteria preparation of the livestock and poultry fermented feed of present embodiment, add weight percent in the feedstuff raw material to be fermented and be 0.18% livestock and poultry fermented feed composite bacteria preparation, just can start well fermentation, be applicable to simultaneously aerobic fermentation and anaerobically fermenting, more suitable for the anaerobically fermenting system.
In the present embodiment, described anaerobism milk-acid bacteria is faecium; In the present embodiment, with faecium by equivalent weight than replacing with a kind of in thermophilus streptococcus, the enterococcus faecalis, perhaps according to equivalent weight than the mixture that replaces with in thermophilus streptococcus, enterococcus faecalis, the faecium, all obtain qualified livestock and poultry fermented feed composite bacteria preparation, products obtained therefrom character there is no significant difference.
The livestock and poultry fermented feed composite bacteria preparation that present embodiment makes, water content is 0.7%, deposits 4 months under vacuum-packed condition, and saccharomycetic quantity is substantially constant, and the quantity of living lactic acid bacteria is kept at 55%.
Embodiment six
A. prepare liquid lactic acid bacterium culture medium to be inoculated: with brown sugar and not diluted and do not contain antibiotic milk by weight mixing to brown sugar and dissolve fully for brown sugar: milk=1:21, then be divided in the triangular flask, bottleneck seals with tampon, outsourcing kraft paper sealed storage, and temperature be under 108 ℃ the sterilization 21 minutes, then be cooled to normal temperature and be liquid lactic acid bacterium culture medium to be inoculated; Brown sugar also can have cane sugar substitution, can reach experiment purpose equally.
B. the inoculum for preparing liquid lactic acid bacterium culture medium: to antibiotic-free milk wt water doubly milk is diluted with 1 times, then adding weight ratio is sucrose: be stirred to sucrose after the sucrose of milk diluent=1:26 and dissolve fully, solution is contained in the vial, with the airtight glass bottle opening of rubber plug, this mixed solution is cooled to normal temperature after temperature is 118 ℃ of lower sterilizations 18 minutes transfer to later on the Bechtop, then opening bottle closure of rubber adding weight ratio is mixed solution: the anaerobism milk-acid bacteria of anaerobism milk-acid bacteria=50:0.07 is also used the airtight bottleneck of rubber plug, transfer in the constant incubator, and be 39 ℃ of lower constant temperature culture 25 hours in temperature, namely get inoculum; If the ripe untimely usefulness of kind liquid need to be kept in 4 ℃ of refrigerators, validity period is 10 days.
C. (rear sealed storage is that 43 ℃ of lower constant temperature tranquillization are cultured to substratum and occur significantly solidifying shape in temperature, namely gets lactic acid bacteria culture solution with the inoculum among the liquid lactic acid bacterium culture medium among the step a access step b;
D. the kiln malt powder that the lactic acid bacteria culture solution among the step c is added 33 lactic acid bacteria culture solution times of weight adsorbed 3 minutes, namely the malt meal of 33 times of weight mixes with the lactic acid bacteria culture solution of 1 times of weight, then the bread yeast powder mix and blend 1 minute that adds lactic acid bacteria culture solution times of weight after 1.9 absorption, namely the lactic acid bacteria culture solution of the bread yeast powder of 1.9 times of weight and 1 times of weight (after the absorption) mixes, bread microzyme adopts every gram bread yeast dry powder to contain the active yeast of 10,000,000,000 cfu, make livestock and poultry fermented feed composite bacteria preparation, composite bacteria preparation is carried out vacuum bagging preserve.
The application of composite bacteria preparation of the livestock and poultry fermented feed of present embodiment, add weight percent in the feedstuff raw material to be fermented and be 0.2% livestock and poultry fermented feed composite bacteria preparation, just can start well fermentation, be applicable to simultaneously aerobic fermentation and anaerobically fermenting, more suitable for the anaerobically fermenting system.
In the present embodiment, described anaerobism milk-acid bacteria is enterococcus faecalis; In the present embodiment, with enterococcus faecalis by equivalent weight than replacing with a kind of in thermophilus streptococcus, the faecium, perhaps according to equivalent weight than the mixture that replaces with in thermophilus streptococcus, enterococcus faecalis, the faecium, all obtain qualified livestock and poultry fermented feed composite bacteria preparation, products obtained therefrom character there is no significant difference.
The livestock and poultry fermented feed composite bacteria preparation that present embodiment makes, water content is 0.75%, deposits 6 months under vacuum-packed condition, and saccharomycetic quantity is substantially constant, and the quantity of living lactic acid bacteria is kept at 63%.
Embodiment seven
A. prepare liquid lactic acid bacterium culture medium to be inoculated: with brown sugar and not diluted and do not contain antibiotic milk by weight mixing to brown sugar and dissolve fully for brown sugar: milk=1:21, then be divided in the triangular flask, bottleneck seals with tampon, outsourcing kraft paper sealed storage, and temperature be under 114 ℃ the sterilization 19 minutes, then be cooled to normal temperature and be liquid lactic acid bacterium culture medium to be inoculated; Brown sugar also can have cane sugar substitution, can reach experiment purpose equally.
B. the inoculum for preparing liquid lactic acid bacterium culture medium: to antibiotic-free milk wt water doubly milk is diluted with 2 times, then adding weight ratio is sucrose: be stirred to sucrose after the sucrose of milk diluent=1:26 and dissolve fully, solution is contained in the vial, with the airtight glass bottle opening of rubber plug, this mixed solution is cooled to normal temperature after temperature is 121 ℃ of lower sterilizations 22 minutes transfer to later on the Bechtop, then opening bottle closure of rubber adding weight ratio is mixed solution: the anaerobism milk-acid bacteria of anaerobism milk-acid bacteria=50:0.1 is also used the airtight bottleneck of rubber plug, transfer in the constant incubator, and be 36 ℃ of lower constant temperature culture 25 hours in temperature, namely get inoculum; If the ripe untimely usefulness of kind liquid need to be kept in 4 ℃ of refrigerators, validity period is 10 days.
C. with sealed storage behind the inoculum among the liquid lactic acid bacterium culture medium among the step a access step b, be that 38 ℃ of lower constant temperature tranquillization are cultured to substratum and occur significantly solidifying shape in temperature, namely get lactic acid bacteria culture solution;
D. the kiln malt powder that the lactic acid bacteria culture solution among the step c is added 36 lactic acid bacteria culture solution times of weight adsorbed 3 minutes, namely the malt meal of 36 times of weight mixes with the lactic acid bacteria culture solution of 1 times of weight, then the bread yeast powder mixing that adds the rear lactic acid bacteria culture solution times of weight of 2 absorption was stirred 7 minutes, namely the lactic acid bacteria culture solution of the bread yeast powder of 2 times of weight and 1 times of weight (after the absorption) mixes, bread microzyme adopts every gram bread yeast dry powder to contain the active yeast of 10,000,000,000 cfu, make livestock and poultry fermented feed composite bacteria preparation, composite bacteria preparation is carried out vacuum bagging preserve.
The application of composite bacteria preparation of the livestock and poultry fermented feed of present embodiment, add weight percent in the feedstuff raw material to be fermented and be 0.2% livestock and poultry fermented feed composite bacteria preparation, just can start well fermentation, be applicable to simultaneously aerobic fermentation and anaerobically fermenting, more suitable for the anaerobically fermenting system.
In the present embodiment, described anaerobism milk-acid bacteria is thermophilus streptococcus; In the present embodiment, with thermophilus streptococcus by equivalent weight than replacing with a kind of in enterococcus faecalis, the faecium, perhaps according to equivalent weight than the mixture that replaces with in thermophilus streptococcus, enterococcus faecalis, the faecium, all obtain qualified livestock and poultry fermented feed composite bacteria preparation, products obtained therefrom character there is no significant difference.
The livestock and poultry fermented feed composite bacteria preparation that present embodiment makes, water content is 0.75%, deposits 6 months under vacuum-packed condition, and saccharomycetic quantity is substantially constant, and the quantity of living lactic acid bacteria is kept at 62%.
Embodiment eight
A. prepare liquid lactic acid bacterium culture medium to be inoculated: with brown sugar and not diluted and do not contain antibiotic milk by weight mixing to brown sugar and dissolve fully for brown sugar: milk=1:20, then be divided in the triangular flask, bottleneck seals with tampon, outsourcing kraft paper sealed storage, and temperature be under 108 ℃ the sterilization 18 minutes, then be cooled to normal temperature and be liquid lactic acid bacterium culture medium to be inoculated; Brown sugar also can have cane sugar substitution, can reach experiment purpose equally.
B. the inoculum for preparing liquid lactic acid bacterium culture medium: to antibiotic-free milk wt water doubly milk is diluted with 1 times, then adding weight ratio is sucrose: be stirred to sucrose after the sucrose of milk diluent=1:30 and dissolve fully, solution is contained in the vial, with the airtight glass bottle opening of rubber plug, this mixed solution is cooled to normal temperature after temperature is 124 ℃ of lower sterilizations 26 minutes transfer to later on the Bechtop, then opening bottle closure of rubber adding weight ratio is mixed solution: the anaerobism milk-acid bacteria of anaerobism milk-acid bacteria=50:0.1 is also used the airtight bottleneck of rubber plug, transfer in the constant incubator, and be 40 ℃ of lower constant temperature culture 23 hours in temperature, namely get inoculum; If the ripe untimely usefulness of kind liquid need to be kept in 4 ℃ of refrigerators, validity period is 10 days.
C. with sealed storage behind the inoculum among the liquid lactic acid bacterium culture medium among the step a access step b, be that 39 ℃ of lower constant temperature tranquillization are cultured to substratum and occur significantly solidifying shape in temperature, namely get lactic acid bacteria culture solution;
D. the kiln malt powder that the lactic acid bacteria culture solution among the step c is added 37 lactic acid bacteria culture solution times of weight adsorbed 3 minutes, namely the malt meal of 37 times of weight mixes with the lactic acid bacteria culture solution of 1 times of weight, then the bread yeast powder mixing that adds the rear lactic acid bacteria culture solution times of weight of 1 absorption was stirred 6 minutes, namely the lactic acid bacteria culture solution of the bread yeast powder of 1 times of weight and 1 times of weight (after the absorption) mixes, bread microzyme adopts every gram bread yeast dry powder to contain the active yeast of 10,000,000,000 cfu, make livestock and poultry fermented feed composite bacteria preparation, composite bacteria preparation is carried out vacuum bagging preserve.
The application of composite bacteria preparation of the livestock and poultry fermented feed of present embodiment, add weight percent in the feedstuff raw material to be fermented and be 0.1% livestock and poultry fermented feed composite bacteria preparation, just can start well fermentation, be applicable to simultaneously aerobic fermentation and anaerobically fermenting, more suitable for the anaerobically fermenting system.
In the present embodiment, described anaerobism milk-acid bacteria is thermophilus streptococcus; In the present embodiment, with thermophilus streptococcus by equivalent weight than replacing with a kind of in enterococcus faecalis, the faecium, perhaps according to equivalent weight than the mixture that replaces with in thermophilus streptococcus, enterococcus faecalis, the faecium, all obtain qualified livestock and poultry fermented feed composite bacteria preparation, products obtained therefrom character there is no significant difference.
The livestock and poultry fermented feed composite bacteria preparation that present embodiment makes, water content is 0.65%, deposits 6 months under vacuum-packed condition, and saccharomycetic quantity is substantially constant, and the quantity of living lactic acid bacteria is kept at 58%.
In above-described embodiment, bread microzyme powder, milk-acid bacteria and malt meal are the outsourcing product, and milk-acid bacteria is the anaerobic type milk-acid bacteria.And for above technique effect, the proportioning of embodiment one and processing parameter, result of use is best, obviously is better than other embodiment, is the best.
This shows that the livestock and poultry fermented feed of aforesaid method preparation can be deposited under vacuum-packed condition 3-6 month with composite bacteria preparation, saccharomycetic quantity is substantially constant, and the quantity of living lactic acid bacteria is kept at about 50-65%.This composite bacteria product inoculates 0.1～0.2% in the livestock and poultry fermented feed just can start fermentation well, is applicable to simultaneously aerobic fermentation and anaerobically fermenting, more suitable for the anaerobically fermenting system.
Explanation is at last, above embodiment is only unrestricted in order to technical scheme of the present invention to be described, although with reference to preferred embodiment the present invention is had been described in detail, those of ordinary skill in the art is to be understood that, can make amendment or be equal to replacement technical scheme of the present invention, and not breaking away from aim and the scope of technical solution of the present invention, it all should be encompassed in the middle of the claim scope of the present invention.
Claims (9)
1. a livestock and poultry fermented feed is with the preparation method of composite bacteria preparation, it is characterized in that: described composite bacteria preparation is the composite bacteria preparation that contains bread microzyme and milk-acid bacteria, the anaerobism milk-acid bacteria of described milk-acid bacteria for adopting the high density liquid anaerobism to cultivate, the preparation method of described composite bacteria preparation may further comprise the steps:
A. prepare liquid lactic acid bacterium culture medium to be inoculated: brown sugar and milk are dissolved also sealed storage fully by weight mix to brown sugar for brown sugar: milk=1:19-22, and temperature be under 105-115 ℃ the sterilization 18-22 minute, then be cooled to normal temperature and be liquid lactic acid bacterium culture medium to be inoculated;
B. the inoculum for preparing liquid lactic acid bacterium culture medium: doubly to milk wt water doubly milk is diluted with 1-3, then adding weight ratio is sucrose: be stirred to sucrose after the sucrose of milk diluent=1:25-30 and dissolve fully, this mixed solution after being 115-125 ℃ of lower the sterilization 18-22 minute, temperature is cooled to normal temperature, then add the milk-acid bacteria that weight ratio is mixed solution: milk-acid bacteria=50:0.05-0.1, and be 35-40 ℃ of lower constant temperature culture 22-26 hour in temperature, namely get inoculum;
C. with sealed storage behind the inoculum among the liquid lactic acid bacterium culture medium among the step a access step b, be that 35-45 ℃ of lower constant temperature tranquillization is cultured to substratum and occurs significantly solidifying shape in temperature, namely get lactic acid bacteria culture solution;
D. the kiln malt powder that the lactic acid bacteria culture solution among the step c is added 30-40 lactic acid bacteria culture solution times of weight adsorbed 3-7 minute, then add bread yeast powder mix and blend 1-4 minute of lactic acid bacteria culture solution times of weight after the 1-2 absorption, make and produce livestock and poultry fermented feed composite bacteria preparation.
2. livestock and poultry fermented feed according to claim 1 is with the preparation method of composite bacteria preparation, it is characterized in that: among the step a, brown sugar and milk are dissolved also sealed storage fully by weight mix to brown sugar for brown sugar: milk=1:20, and temperature be under 110 ℃ the sterilization 20 minutes, then be cooled to normal temperature and be liquid lactic acid bacterium culture medium to be inoculated.
3. livestock and poultry fermented feed according to claim 2 is with the preparation method of composite bacteria preparation, and it is characterized in that: among the step a, described milk is not for containing the milk of microbiotic and not diluted.
4. livestock and poultry fermented feed according to claim 3 is with the preparation method of composite bacteria preparation, it is characterized in that: among the step b, with 2 times of water to milk wt milk is diluted, then adding weight ratio is sucrose: be stirred to sucrose after the sucrose of milk diluent=1:28 and dissolve fully, this mixed solution after being 120 ℃ of lower sterilizations 20 minutes, temperature is cooled to normal temperature, then add the milk-acid bacteria that weight ratio is mixed solution: milk-acid bacteria=50:0.08, and be 37 ℃ of lower constant temperature culture 24 hours in temperature, namely get inoculum.
5. livestock and poultry fermented feed according to claim 4 is with the preparation method of composite bacteria preparation, it is characterized in that: among the step c, with sealed storage behind the inoculum among the access of the liquid lactic acid bacterium culture medium among the step a step b, be that 40 ℃ of lower constant temperature tranquillization are cultured to substratum and occur significantly solidifying shape in temperature, namely get lactic acid bacteria culture solution.
6. livestock and poultry fermented feed according to claim 5 is with the preparation method of composite bacteria preparation, it is characterized in that: in the steps d, the kiln malt powder that lactic acid bacteria culture solution among the step c is added 35 lactic acid bacteria culture solution times of weight adsorbed 5 minutes, then add the bread yeast powder mix and blend 3 minutes of lactic acid bacteria culture solution times of weight after 1.5 absorption, make and produce livestock and poultry fermented feed composite bacteria preparation.
7. livestock and poultry fermented feed according to claim 6 is with the preparation method of composite bacteria preparation, and it is characterized in that: described anaerobism milk-acid bacteria comprises one or more mixtures in thermophilus streptococcus (Streptococcus thermophilus), enterococcus faecalis (Enterococcus.faecalis) and the faecium (Enterococcus.faecium).
8. livestock and poultry fermented feed according to claim 7 is with the preparation method of composite bacteria preparation, and it is characterized in that: described bread microzyme adopts every gram bread yeast dry powder to contain the active yeast of 10,000,000,000 cfu.
9. the livestock and poultry fermented feed that makes according to claim 8 is characterized in that with the application of composite bacteria preparation: adding weight percent in the feedstuff raw material to be fermented is the livestock and poultry fermented feed composite bacteria preparation of 0.1-0.2%.
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