CN106614789A - Preparation of three tartary buckwheat stalk extracts, and application of insecticidal activity of extracts - Google Patents
Preparation of three tartary buckwheat stalk extracts, and application of insecticidal activity of extracts Download PDFInfo
- Publication number
- CN106614789A CN106614789A CN201611106030.6A CN201611106030A CN106614789A CN 106614789 A CN106614789 A CN 106614789A CN 201611106030 A CN201611106030 A CN 201611106030A CN 106614789 A CN106614789 A CN 106614789A
- Authority
- CN
- China
- Prior art keywords
- extract
- extracts
- duck wheat
- stalk
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/30—Polygonaceae [Buckwheat family], e.g. red-knees or rhubarb
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Agronomy & Crop Science (AREA)
- Microbiology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Plant Pathology (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The insecticidal activity of three tartary buckwheat stalk extracts belongs to the field of pesticide. The invention provides extracts of three tartary buckwheat stalks such as KX2008-07, Haizigua and Ejiaodouyou, wherein the extracts have insecticidal action on prodenia litura and can be used for preparing botanical insecticide. The extracts adopt the tartary buckwheat stalks as the raw materials, utilize waste, do not damage beneficial organisms, avoid environmental pollution, are easy to degrade, and are extremely low in cost and suitable for popularization.
Description
Technical field
The invention belongs to pesticide field, and in particular to KX2008-07, lake Gua, frontal eminence beans imprison the duck wheat of three kinds of kinds
The insecticidal activity of stalk extract, while providing the extract of these three plants and its preparation method of plant insecticide.
Background technology
With the spread of the intensive cropping system of modern agriculture and continuing on for chemical insecticide of all kinds,
The abuse of a large amount of chemical pesticides, although having efficiently controlled the generation and harm of insect pest to a certain extent, while also to big
The beneficial organism of amount causes certain killing, wherein just including the natural enemy of some insects, so as to cause the continuous ferocious of insect to stick up, worm
Harmful generation is more frequently and intensive.On the other hand, chemical pesticide is frequently used, and also further results in insect to chemical pesticide
Produce increasingly serious drug resistance.Efficiently, low toxicity, it is degradable, be that pesticide in future is sent out to beneficial organism and livestock and poultry, human-body safety
Exhibition direction, plant source agricultural is one of those important monoid.
Botanical pesticide is also known as botanical pesticide.Using the agricultural chemicals of plant resource explorationses, botanical pesticide is included from plant
The active component of middle extraction, plant itself and the compound synthesized by active structure and derivative.Classification has phytotoxin, plant
Endogenous hormones, botanical insect hormone, anti-feedant, attractant, repellant, chemosterilant, synergist, plant defense are plain, different strain gram
Biomass etc..
KX2008-07 (indicating lower Latin or source), lake Gua (indicating lower Latin or source), the deep and remote (note of frontal eminence beans
Bright lower Latin or source) it is three kinds of bitter buckwheat wheat varieties, inventor has found the duck wheat extract of these three kinds through research
This agricultural pests strong in agriculturally very harmful and drug resistance of prodenia litura can be killed.At present it is not yet found that these three are bitter
The report of the insecticidal activity of buckwheat extract.Present invention firstly discovers that the extract of these three duck wheats can be used to kill insect,
Development and application for new type natural plant insecticide provides new active ingredient.Additionally, the material that the present invention is selected is
Three kinds of duck wheat stalks.Its wide material sources and for produce waste material, this research twice laid, solve crop straw burning bring environment dirt
Development and application of the dye problem also for new type natural plant insecticide provide theoretical foundation.
The content of the invention
To solve problems of the prior art the invention reside in providing kx008-07, lake Gua, frontal eminence beans imprisons three kinds
The insecticidal activity of duck wheat stalk extract, while providing the extract of these three plants and its preparation side of plant insecticide
Method.
It is an object of the invention to provide a kind of duck wheat stalk insect-killing extract and preparation method thereof.The present invention's
The two of purpose are to provide a kind of bio-insecticide composition with the extract as active ingredient.The third object of the present invention
It is to provide application of the extract in biological insecticides are prepared.
The present invention compares three kinds of kinds（KX008-07, lake Gua, frontal eminence beans are imprisoned）Duck wheat stalk extract kill
Worm activity, it is determined that extract with insecticidal activity and preparation method thereof.
Three kinds of duck wheats stalk extract of the present invention is obtained through extraction, concentration, series extraction, its preparation side
Method is comprised the following steps：Duck wheat stalk meal is taken, is soaked with ethanol, ultrasonic wave hydrotropy is extracted, filtered, filter residue repeats above-mentioned carrying
Step is taken, is filtered, merge extract, reduced pressure concentration was both obtained.
The preferred 50-95% of ethanol used, most preferably 70% ethanol.
The present invention also provides the plant insecticide composition of each extract for preparing in this way.The Biocidal
Agent composition is, with the extract of the present invention as active ingredient, to be constituted with acceptable carrier on agricultural chemicals, and with suitable agriculture
Exist with dosage form, these preparations can be solution, spray etc..
Present invention also offers the effective ingredient with three kinds of duck wheat cane extracts as plant insecticide, with weight portion
Meter, content is respectively：Extract dry extract 40-75 parts, solvent 70-100 parts, surfactant 1-10 parts.Solvent for use is preferred
Deionized water, the preferred Tween 80 of surfactant used.
Invention further provides application of the various extracts in biological insecticides are prepared.The desinsection phase carried out for this
Experiment is closed with killing rate as activity index.Test result indicate that, kx008-07, lake Gua, frontal eminence beans imprison the bitter buckwheat of three kinds of kinds
Wheat cane ethanol extract is to prodenia litura（Prodenia litura（Fabricius）With different degrees of insecticidal activity,
Can be used to prepare plant insecticide.
Description of the drawings
The stomach toxicity insecticidal effect of tri- kinds of duck wheat stalk extract insecticides of Fig. 1.
The insecticidal effect of tagging of tri- kinds of duck wheat stalk extract insecticides of Fig. 2.
Specific embodiment
The present invention is illustrated below by way of specific embodiment, but the present invention is not only following whole embodiments
The extracting method of three kinds of duck wheat stalk extracts is comprised the following steps described in embodiment 1：
（1）Take three kinds of duck wheat stalks for being collected in Liangshan, Sichuan sound of a roaring fire in 55 DEG C of baking ovens and do and shred to embrittlement；
（2）The ethanol that a certain amount of stalk adds 30 times of amounts 70% is taken, in the triangular flask with grinding port plug（25℃）Extract 12 h
Ultrasonic wave extraction 30min afterwards, suction filtration obtains filtrate；
（3）It is 53 DEG C of vacuum distillation recovered solvents to control temperature on a rotary evaporator, is condensed into medicinal extract,
And medicinal extract is dried to constant weight at 55 DEG C；
（4）Preserve at 4 DEG C.
Recovery rate is about 11%.
Recovery rate=dry extract quality/crude drug quality.
The preparation of the KX2008-07 stalk extract insecticides of embodiment 2
（1）1.0g Tween 80s and 99 deionized waters are stirred 5 minutes, the aqueous solution of 1% Tween 80 is obtained；
（2）55g KX2008-07 stalk extract dry extracts are weighed, 1/3 above-mentioned Tween solution is added thereto to, normal temperature is sealed
1h is stood, ultrasonication 30min is used afterwards；
（3）Will（1）In points of 2 times additions of remaining Tween solution（2）Resulting mixture, stirs every time 15min, that is, obtain
KX2008-07 stalk extract insecticides.
The preparation of the lake Gua stalk extract insecticides of embodiment 3
（1）1.0g Tween 80s and 99 deionized waters are stirred 5 minutes, the aqueous solution of 1% Tween 80 is obtained；
（2）55g lakes Gua stalk extract dry extracts are weighed, 1/3 above-mentioned Tween solution is added thereto to, sealing normal temperature stands
1h, uses afterwards ultrasonication 30min；
（3）Will（1）In points of 2 times additions of remaining Tween solution（2）Resulting mixture, stirs every time 15min, that is, obtain
Lake Gua stalk extract insecticides.
The preparation of the deep and remote stalk extract insecticide of the frontal eminence beans of embodiment 4
（1）1.0g Tween 80s and 99 deionized waters are stirred 5 minutes, the aqueous solution of 1% Tween 80 is obtained；
（2）The deep and remote stalk extract dry extract of 55g frontal eminences beans is weighed, 1/3 above-mentioned Tween solution is added thereto to, sealing normal temperature is quiet
1h is put, ultrasonication 30min is used afterwards；
（3）Will（1）In points of 2 times additions of remaining Tween solution（2）Resulting mixture, stirs every time 15min, that is, obtain
The deep and remote stalk extract insecticide of frontal eminence beans.
The stomach poison function of 5 three kinds of duck wheat stalk extract insecticides of embodiment
Insecticidal activity experiment is carried out using the duck wheat stalk extract insecticide prepared in embodiment 2-4.
Obtained insecticide is diluted into respectively 2 quilts and 4 times.10g feeds are weighed, the liquid of 300 μ l is added thereto to, is mixed
It is even, uniform application in the culture dish of a diameter of 8cm, then by the children of homogeneous, the healthy prodenia litura of 3 age sizes after hungry 4h
Worm accesses culture dish.Each process is repeated 3 times, 10 every time, and the aqueous solution with 1% Tween 80 of equivalent is as blank.Most
After be placed in temperature for 26 DEG C, periodicity of illumination 16: 8 (L: D), humidity is to cultivate in 70% constant temperature illumination box, and in 24h,
Observation statistics, is as a result shown in Fig. 1 after 48h.
Computing formula：
The action of contace poison of 6 three kinds of duck wheat stalk extract insecticides of embodiment
Insecticidal activity experiment is carried out using the duck wheat stalk extract insecticide prepared in embodiment 2-4.
Obtained insecticide is diluted into respectively 2 quilts and 4 times.Tag and adopt drop method, with liquid-transfering gun in per three ages children
The μ l liquids of pronotary drop 1 of worm, make back be fully contacted liquid, after solvent volatilization, in accessing culture dish.Each process
Repeat 3 times, 10 cephalont every time, the aqueous solution with 1% Tween 80 of equivalent is as blank.Train in constant temperature illumination box
Support, 26 DEG C of cultivation temperature, periodicity of illumination 16: 8 (L: D), humidity is 70%, and 24h, 48h are observed and counted after process, knot
Fruit sees Fig. 2.
Computing formula：
Conclusion：Can be drawn by Fig. 2,2 times of dilution killing rates of the made insecticide of KX2008-07 dry extracts are up to 93.33%,
Up to 33.33%, frontal eminence beans imprison 2 times of the made insecticide of dry extract to 2 times of dilution killing rates of the made insecticide of lake Gua dry extracts
Dilution killing rate up to 36.67%, so when desinsection mode is that three kinds of duck wheat cane extracts are respectively provided with certain killing when tagging
Worm activity, the wherein made insecticide best results of KX2008-07 dry extracts.
In sum, plant prepared by the deep and remote three kinds of duck wheat cane extracts of KX008-07, lake Gua, frontal eminence beans is selected to kill
Worm agent is respectively provided with certain insecticidal action, can be used for the preparation of plant insecticide, wherein selecting KX2008-07 dry extracts institute
Insecticide effect processed is preferable.
Finally it should be noted that:The above is a part of example of the present invention, is not limited to the present invention, to the greatest extent
Pipe is described in detail with reference to the foregoing embodiments to the present invention, and for a person skilled in the art, it still can be with
The technical scheme that foregoing embodiments are recorded is improved, or equivalent is carried out to some technical characteristics therein.It is all
Within the spirit and principles in the present invention, any modification, equivalent substitution and improvements etc. are done, the protection of the present invention is should be included in
Within the scope of.
Claims (6)
- The deep and remote duck wheat stalk of 1.KX2008-07 duck wheat stalk extracts, lake Gua duck wheat stalk extracts and frontal eminence beans Extract is used to prepare insecticide.
- 2. a kind of preparation method of the stalk extract of duck wheat according to claim 1, three kinds of duck wheat stalks are extracted Thing is obtained through extraction, concentration, series extraction, and concrete preparation process includes：Raw material duck wheat stalk meal is taken, is soaked with ethanol Bubble, ultrasonic wave hydrotropy is extracted, and is filtered, and filter residue repeat the above steps are extracted, and is filtered, and merges extract, and reduced pressure concentration is drying to obtain Medicinal extract.
- 3. the preparation method described in claim 2, ethanol preferred volume concentration used is 70% ethanol.
- 4. a kind of plant insecticide composition, including active ingredient is extraction prepared by the arbitrary methods described of claim 2-3 Thing, in parts by weight, concrete component includes：Claim 2-3 arbitrary described extract dry extract 40-75 parts, solvent 70- 100 parts, surfactant 1-10 parts.
- 5. the plant insecticide described in a kind of claim 4, wherein the preferred deionized water of the solvent, surfactant used It is preferred that Tween 80.
- 6. electuary is killed according to the arbitrary described plant source of claim 4-5, its formulation is solution, spray.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611106030.6A CN106614789B (en) | 2016-12-06 | 2016-12-06 | The preparation of three kinds of tartary buckwheat stalk extracts and its application of insecticidal activity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611106030.6A CN106614789B (en) | 2016-12-06 | 2016-12-06 | The preparation of three kinds of tartary buckwheat stalk extracts and its application of insecticidal activity |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106614789A true CN106614789A (en) | 2017-05-10 |
| CN106614789B CN106614789B (en) | 2019-08-16 |
Family
ID=58818831
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611106030.6A Expired - Fee Related CN106614789B (en) | 2016-12-06 | 2016-12-06 | The preparation of three kinds of tartary buckwheat stalk extracts and its application of insecticidal activity |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106614789B (en) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104206434A (en) * | 2014-08-27 | 2014-12-17 | 青岛保利康新材料有限公司 | High-efficiency insecticidal pesticide |
-
2016
- 2016-12-06 CN CN201611106030.6A patent/CN106614789B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104206434A (en) * | 2014-08-27 | 2014-12-17 | 青岛保利康新材料有限公司 | High-efficiency insecticidal pesticide |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106614789B (en) | 2019-08-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104472527B (en) | A kind of have the dinotefuran insecticide luring worm effect | |
| CN103081912B (en) | Celastrus angulatus and cnidium lactone compounded pesticide and preparation method thereof | |
| CN106538597B (en) | A kind of nematicidal composition containing fosthiazate | |
| CN103141488B (en) | Pesticide compounded by azadirachtin and cnidium lactone and preparation method thereof | |
| CN105076214B (en) | A kind of method for improving entomopathogenic nematode prevention fragrant-flowered garlic maggot effect | |
| KR20130114537A (en) | Due to the complex at the plant pest control for natural composition and manufacturing method | |
| CN101317572A (en) | Mixed plant source insecticide | |
| CN101984828A (en) | Compound pesticide | |
| CN106490070A (en) | A kind of biological insecticides of preventing and treating rice leaf roller and preparation method thereof | |
| CN104920487A (en) | Traditional Chinese medicine extract, preparation method thereof and method of utilizing same to prevent and control wheat aphid | |
| CN103004854A (en) | Allyl isothiocyanate and nicotine compound insecticide and application thereof | |
| CN102657216A (en) | Use of pesticide mixed preparation containing chlorpyrifos and high-efficiency cypermethrin in control of Athetis lepigone Moschler | |
| CN104705350B (en) | A kind of white sand parthenium extract and its preparation method and application | |
| CN106900757B (en) | A kind of bitter pueraria root extract and the Pesticidal combination of Osthole and application thereof for preventing and treating agricultural pests | |
| CN103931649B (en) | The Pesticidal combination of emamectin-benzoate and cholrempenthrin | |
| CN106614789A (en) | Preparation of three tartary buckwheat stalk extracts, and application of insecticidal activity of extracts | |
| CN101919416B (en) | Method for extracting insecticidal active material from traditional Chinese medicine feather cockscomb and application thereof | |
| CN106577804B (en) | A kind of shielding protection type insecticide of repellent natural enemy insect and honeybee | |
| CN101305717B (en) | Insecticidal spray EC and its preparation method | |
| CN101396026A (en) | Plant pesticide capable of preventing and treating stored grain insects and use thereof | |
| CN101322501A (en) | Granulosis virus and Bacillus thuringiensis wettable powder for disinsection of imported cabbageworm | |
| CN106973949B (en) | A kind of Pesticidal combination and application thereof comprising bitter pueraria root extract and matrine | |
| CN103109846B (en) | Dichlorovos kadethrine compounded pesticide | |
| CN107156203A (en) | Prevent and treat the composition, preventing and treating root nematode preparation and its application of root-knot nematode | |
| CN105875610A (en) | Application of Acnistus A in insecticide preparation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190816 Termination date: 20211206 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |