CN106614768B - The method extracted algistatic activity ingredient from purslane seed and be used to prepare algae-inhibiting agent - Google Patents
The method extracted algistatic activity ingredient from purslane seed and be used to prepare algae-inhibiting agent Download PDFInfo
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- CN106614768B CN106614768B CN201611216111.1A CN201611216111A CN106614768B CN 106614768 B CN106614768 B CN 106614768B CN 201611216111 A CN201611216111 A CN 201611216111A CN 106614768 B CN106614768 B CN 106614768B
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- purslane seed
- algae
- purslane
- inhibiting agent
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
Abstract
A kind of method the present invention relates to natural algistatic activity constituents extraction and for manufacturing algae-inhibiting agent.A method of it extracting algistatic activity ingredient from purslane seed and is used to prepare algae-inhibiting agent, first by pretreated purslane seed and ethyl acetate according to quality: volume ratio mixes for the ratio of 1:10~30(kg:L)；Then leaching liquor is handled, removal purslane seed residue obtains purslane seed acetic acid ethyl acetate extract, then recycles ethyl acetate through being concentrated under reduced pressure, and obtains purslane seed ethyl acetate extract, this i.e. final algae-inhibiting agent obtained.In use, being added algae-inhibiting agent obtained dimethyl sulfoxide hydrotropy using cyanobacteria to place 48~120 hours in the Small-scale Landscape Water Bodies of sociales, so that the algae in algae-inhibiting agent and water contacts, achieve the purpose that inhibit algal grown.The present invention extracts algae-inhibiting agent made from natural algistatic activity ingredient from purslane seed, belongs to natural component, degradable in water body for controlling algae, not will cause secondary pollution.
Description
Technical field
The present invention relates to a kind of for inhibiting the natural algistatic activity component extracting method of algal grown, more particularly, to one
Kind extracts the method that natural algistatic activity ingredient is used to prepare algae-inhibiting agent from purslane seed.
Background technique
The algae of large area breaks out, and seriously destroys the balance and stability of aquatic ecosystem, to the production and life of the mankind
Work causes extreme influence, and algae toxin has constituted the threat to environment for human survival.
It is a kind of novel biological algal control technology, the good, expense with effect using phyto-allelopathic algae control
Low, material is naturally easy to get, does not easily cause the advantages that secondary pollution.
Summary of the invention
The present invention provides one kind to extract natural algistatic activity ingredient from purslane seed and prepare for being excellent with cyanobacteria
The method of the natural algae-inhibiting agent of the Small-scale Landscape Water Bodies of gesture kind.The natural algae-inhibiting agent of purslane seed of preparation, can effectively inhibit water
Middle algal grown, algae-inhibiting agent obtained, belongs to natural component, degradable in water body, and the influence to environment is small, without secondary dirt
Dye, suitable for removing algae by the Small-scale Landscape Water Bodies of sociales of cyanobacteria.
The technical solution adopted in the present invention:
A method of it extracting algistatic activity ingredient from purslane seed and is used to prepare algae-inhibiting agent, comprise the following steps that
(1) by pretreated purslane seed and organic solvent ethyl acetate according to quality: volume ratio is 1:10~30
(kg:L) ratio mixing, is placed 12~36 hours, to promote active constituent contained in purslane seed to be fully dissolved out；
(2) leaching liquor is handled, removal purslane seed residue obtains purslane seed extracting solution, then is concentrated under reduced pressure back
Ethyl acetate is received to get purslane seed ethyl acetate extract is arrived, this is final algae-inhibiting agent obtained.
The described method extracted algistatic activity ingredient from purslane seed and be used to prepare algae-inhibiting agent, in step (1), institute
It states the process that pretreated step includes the cleaning of purslane seed, dries or dry, crushes: purslane seed use is boiled into oneself cooling
Water, distilled water or ultrapure water clean, naturally dry, obtains dry purslane seed material, being then crushed to granular size is 20
~40 mesh.
The described method extracted algistatic activity ingredient from purslane seed and be used to prepare algae-inhibiting agent in step (2), makes
The particulate matter residue in leaching liquor is filtered out with qualitative filter paper.
Beneficial effects of the present invention:
1, the present invention extracts the method that natural algistatic activity ingredient prepares algae-inhibiting agent, algae-inhibiting agent obtained from purslane seed
Belong to natural component, it is degradable in water body, it not will cause secondary pollution.
2, the natural algae-inhibiting agent of purslane seed prepared by the present invention, can effectively inhibit algal grown in water, to the shadow of environment
Sound is small, without secondary pollution, suitable for removing algae by the Small-scale Landscape Water Bodies of sociales of cyanobacteria.The present invention it is practical, control algae at
This low, application easy to spread.
3, the raw materials used in the present invention is purslane seed, to propose new approach for its resource utilization；This hair simultaneously
The algae-inhibiting agent of many advantages, such as bright algae-inhibiting agent has extraction process simple, easy to operate, extraction is imitated with good algal control
Fruit can effectively remove algae in water, and new method is provided for algae removal.
Specific embodiment
Below by specific embodiment, technical scheme of the present invention will be described in further detail.
Embodiment 1
The method that the present invention extracts algistatic activity ingredient from purslane seed and is used to prepare algae-inhibiting agent, including step is such as
Under:
(1) by purslane seed with boil cooling tap water or ultrapure water clean, naturally dry, obtain dry purslane seed
Material, being then crushed to granular size is 20~40 mesh；
(2) by pretreated purslane seed and ethyl acetate according to quality: volume ratio be 1:10~30(kg:L) ratio
Example mixing, and placement 12~36 hours after mixing evenly, preferably 24 hours, to promote active constituent contained in purslane seed to fill
Divide dissolution；
(3) leaching liquor is handled, removal purslane seed residue obtains purslane seed extracting solution, then is concentrated under reduced pressure back
Ethyl acetate is received to get purslane seed ethyl acetate extract is arrived, this is final algae-inhibiting agent obtained.
When for inhibiting algal grown in water, by algae-inhibiting agent obtained dimethyl sulfoxide hydrotropy, it is excellent for being added with cyanobacteria
It in the Small-scale Landscape Water Bodies of gesture kind, places 48~120 hours, so that the algae in algae-inhibiting agent and water contacts, reaches and inhibit algae
The purpose of growth.In use, being determined by experiment algae-inhibiting agent dosage according to frustule quantity in water body.
Embodiment 2
The method that the present embodiment extracts natural algistatic activity ingredient from purslane seed, comprises the following steps that
(1) by pretreated purslane seed and ethyl acetate according to quality: volume ratio be 1:10~30(kg:L) ratio
Example mixing；
(2) leaching liquor being handled using qualitative filter paper, removal purslane seed residue obtains purslane seed extracting solution, then
Ethyl acetate is recycled through being concentrated under reduced pressure, obtains purslane seed ethyl acetate extract, algae-inhibiting agent is finally made.
Embodiment 3
The method that the present embodiment extracts natural algistatic activity ingredient from purslane seed, exists with the difference of embodiment 2
In: in step (1), the pretreated step includes the process that purslane seed cleans, dries or dry, crushes: by purslane
Seed with boil cooling tap water or ultrapure water clean, naturally dry, obtain dry purslane seed material, be then crushed to particle
Size is 20~40 mesh.
Embodiment 4
The method that the present embodiment extracts natural algistatic activity ingredient from purslane seed, it is different from foregoing embodiments it
Be in: by pretreated purslane seed and ethyl acetate according to quality: volume ratio be 1:10 ratio mix.
Embodiment 5
The method that the present embodiment extracts natural algistatic activity ingredient from purslane seed, it is different from foregoing embodiments it
Be in: by pretreated purslane seed and ethyl acetate according to quality: volume ratio be 1:20 ratio mix.
Embodiment 6
The method that the present embodiment extracts natural algistatic activity ingredient from purslane seed, it is different from foregoing embodiments it
Be in: by pretreated purslane seed and ethyl acetate according to quality: volume ratio be 1:30 ratio mix.
Embodiment 7
The method that the present embodiment extracts natural algistatic activity ingredient from purslane seed, it is different from foregoing embodiments it
Be in: by pretreated purslane seed and ethyl acetate according to quality: volume ratio be 1:25 ratio mix.
Embodiment 8
The method that the present embodiment extracts natural algistatic activity ingredient from purslane seed, it is different from foregoing embodiments it
Be in: by pretreated purslane seed and ethyl acetate according to quality: volume ratio be 1:15 ratio mix.
Below with reference to the result of study in laboratory to extracting method, the system of algae-inhibiting agent of the natural algistatic activity ingredient of the present invention
It is standby and its for inhibiting the method and process of algal grown in water to further illustrate.
Experiment 1
Purslane seed is taken to be rinsed well with cooling tap water is boiled, naturally dry, being crushed to granular size is 20 mesh.With
Ethyl acetate is Extraction solvent, and 5.0g purslane powder of seeds is immersed in the ethyl acetate of 100mL, is placed 24 hours, is used
Filter paper handles leaching liquor, and removal purslane seed residue obtains purslane seed acetic acid ethyl acetate extract, then through being concentrated under reduced pressure
Ethyl acetate is recycled to get purslane seed ethyl acetate extract is arrived, this is final algae-inhibiting agent；Again by purslane seed acetic acid
Ethyl ester extract dimethyl sulfoxide hydrotropy；It is 8.3 × 10 that algae-inhibiting agent after taking hydrotropy, which is added to concentration of algae,5In the water body of a/mL,
Make 20 mg/L of ultimate density of algae-inhibiting agent, place 72 h, algal control rate can reach 49.4%.
Experiment 2
Purslane seed is taken to be rinsed well with cooling tap water is boiled, naturally dry, being crushed to granular size is 40 mesh.With
Ethyl acetate is Extraction solvent, and 5.0g purslane powder of seeds is immersed in the ethyl acetate of 150mL, is placed 48 hours, is used
Filter paper handles leaching liquor, and removal purslane seed residue obtains purslane seed acetic acid ethyl acetate extract, then through being concentrated under reduced pressure
Ethyl acetate is recycled to get purslane seed ethyl acetate extract is arrived, this is final algae-inhibiting agent；Again by purslane seed acetic acid
Ethyl ester extract dimethyl sulfoxide hydrotropy；It is 6.7 × 10 that algae-inhibiting agent after taking hydrotropy, which is added to concentration of algae,5In the water body of a/mL,
Make 30 mg/L of ultimate density of algae-inhibiting agent, place 72 h, algal control rate can reach 78.3%.
Experiment 3
Take purslane seed ultrapure water clean, naturally dry, being crushed to granular size is 30 mesh.It is with ethyl acetate
5.0g purslane powder of seeds is immersed in the ethyl acetate of 75mL by Extraction solvent, is placed 36 hours, using filter paper to leaching liquor
It being handled, removal purslane seed residue obtains purslane seed acetic acid ethyl acetate extract, then recycles ethyl acetate through being concentrated under reduced pressure,
Purslane seed ethyl acetate extract is obtained, this is final algae-inhibiting agent；Purslane seed ethyl acetate extract is used again
Dimethyl sulfoxide hydrotropy；It is 4.7 × 10 that algae-inhibiting agent after taking hydrotropy, which is added to concentration of algae,5In the water body of a/mL, make algae-inhibiting agent most
Final concentration of 10 mg/L, places 72 h, and algal control rate can reach 16.5%.
Claims (4)
1. a kind of method for extracting algistatic activity ingredient from purslane seed, comprises the following steps that
(1) by pretreated purslane seed and organic solvent ethyl acetate according to quality: volume ratio is the kg:L's of 1:10~30
Ratio mixing, is placed 12~36 hours, to promote active constituent contained in purslane seed to be fully dissolved out；
(2) leaching liquor is handled, removal purslane seed residue obtains purslane seed extracting solution, then recycles second through being concentrated under reduced pressure
Acetoacetic ester to get arrive purslane seed ethyl acetate extract.
2. the method according to claim 1 for extracting algistatic activity ingredient from purslane seed, it is characterised in that: step
(1) in, the pretreated step includes the cleaning of purslane seed, the process drying or dry, crush: by purslane seed with boiling
Cooling tap water, distilled water or ultrapure water clean, naturally dry, obtains dry purslane seed material, is then crushed to particle
Size is 20~40 mesh.
3. the method according to claim 1 or 2 for extracting algistatic activity ingredient from purslane seed, it is characterised in that: step
Suddenly in (2), the particulate matter residue in leaching liquor is filtered out using qualitative filter paper.
4. a kind of using purslane seed extract as the application of algae-inhibiting agent, the purslane seed extract is according to claim 1
The purslane seed extract of~3 described in any item method preparations that algistatic activity ingredient is extracted from purslane seed.
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| CN201611216111.1A CN106614768B (en) | 2016-12-26 | 2016-12-26 | The method extracted algistatic activity ingredient from purslane seed and be used to prepare algae-inhibiting agent |
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| CN105129949A (en) * | 2015-09-06 | 2015-12-09 | 河南城建学院 | Method for extracting natural flocculation active ingredients from hesperidium peels, method for preparing algaecide and method for removing algae in water through algaecide |
| CN105256766A (en) * | 2015-09-24 | 2016-01-20 | 江苏省林业科学研究院 | Ecology-simulated water bank protection slope structure |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US9131687B2 (en) * | 2010-08-20 | 2015-09-15 | President And Fellows Of Harvard College | Roseobacticides and uses thereof |
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- 2016-12-26 CN CN201611216111.1A patent/CN106614768B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101720790A (en) * | 2009-11-26 | 2010-06-09 | 南开大学 | Preparation method and application of radix dichroae water bloom algae seed growth inhibitor |
| CN101731284A (en) * | 2009-12-03 | 2010-06-16 | 河海大学 | Method for extracting alga-inhibiting active ingredients from Artemisia annua L. and alga-inhibiting method |
| CN102812974A (en) * | 2012-06-14 | 2012-12-12 | 云南大学 | Method for extracting natural algicide from Acorus calamus L and algae inhibition method |
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| CN105129949A (en) * | 2015-09-06 | 2015-12-09 | 河南城建学院 | Method for extracting natural flocculation active ingredients from hesperidium peels, method for preparing algaecide and method for removing algae in water through algaecide |
| CN105256766A (en) * | 2015-09-24 | 2016-01-20 | 江苏省林业科学研究院 | Ecology-simulated water bank protection slope structure |
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Effective date of registration: 20191231 Address after: 314109 Room 501, No. 341, Tianning Avenue, Tianning Town, Jiashan County, Jiaxing City, Zhejiang Province Patentee after: Jiashan Guohong Environmental Protection Technology Co., Ltd Address before: Lung Cheung Road Pingdingshan city Henan province 467036 Patentee before: Henan University of Urban Construction |
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Effective date of registration: 20210205 Address after: No. 168, Changjiang Road, Donghu street, Pizhou City, Xuzhou City, Jiangsu Province Patentee after: Liu Ronghua Address before: 314109 Room 501, 341 Tianning Avenue, Tianning Town, Jiashan County, Jiaxing City, Zhejiang Province Patentee before: Jiashan Guohong Environmental Protection Technology Co.,Ltd. |
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Effective date of registration: 20210210 Address after: Room 601, unit 1, building 13, Yunchen, Chanba Peninsula, Chanhe East Road, Chanba Ecological District, Xi'an, Shaanxi 710000 Patentee after: Shaanxi historical Biotechnology Co.,Ltd. Address before: No. 168, Changjiang Road, Donghu street, Pizhou City, Xuzhou City, Jiangsu Province Patentee before: Liu Ronghua |