CN106613413A - Cotton low-temperature-resistant sprouting identification method - Google Patents
Cotton low-temperature-resistant sprouting identification method Download PDFInfo
- Publication number
- CN106613413A CN106613413A CN201610604492.4A CN201610604492A CN106613413A CN 106613413 A CN106613413 A CN 106613413A CN 201610604492 A CN201610604492 A CN 201610604492A CN 106613413 A CN106613413 A CN 106613413A
- Authority
- CN
- China
- Prior art keywords
- seed
- cotton
- low temperature
- low
- temperature resistant
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Abstract
The invention relates to the cotton breeding field, in particular to a cotton low-temperature-resistant sprouting identification method. The method comprises the steps that seeds are selected; the seeds are pretreated; low-temperature stress treatment is performed; sterilized seeds are placed in a wet environment, and the seeds are subjected to low-temperature stress treatment continuously for 10 days. Low-temperature-resistant level grading is performed. Relative emergence rate is adopted for classification, an objective evaluation indicator is put up, and the error caused by man-made subjective factors is reduced greatly. The operation is simple, the manpower and material resource cost is low, the occupied space is small, and mass materials can be identified.
Description
Technical field
The present invention relates to cotton breeding field, and in particular to the low temperature resistant germination authentication method of cotton.
Background technology
Chinese cotton region " move westwards and go up north " has become undisputable fact, Xinjiang as Cotton in China main producing region because its
Special geographical conditions and climatic environment, low temperature has become one of abiotic stress factor for limiting cotton in Xinjiang production.Newly
There is a climatic characteristic of " early stage heat up slow, post drop fast " in boundary area, and frost-free period is short and spring climate variability, often has significantly
" cold spell in later spring " phenomenon.Cotton belongs to non-Cold exposed crop, and normal growth needs enough heat conditions, and to low-temperature sensitive, growth is sent out
Educate each stage is affected by low temperature, is particularly subjected to relatively low chilling damage in germination period and Seedling Stage, its breeding time
To significantly postpone, and budding, bloom and maturation is postponed, greatly affect ultimate output and quality.Cultivate low temperature resistant cotton new product
It is important approach to plant, therefore, screening, identification and the evaluation for carrying out the low temperature resistant germ plasm resource of cotton is the only way which must be passed.
At present, the research with regard to the low temperature resistant identification of cotton is also little, lacks unified standard of perfection and effectively evaluating body
System, becomes the important limiting factor of the low temperature resistant identification of germplasm resource for cotton.Common low temperature identification report has in other crops
The grasses such as paddy rice, corn, wheat are all unifacial leaf, and cotton is dicotyledon, and cotton originates from Perenniporia martius
Area, is a kind of plant of non-Cold exposed, and this is from arabidopsis, rape, soybean etc., and dicotyledonous crops are also different.Therefore, other works
Existing authentication method can not directly be indiscriminately imitated and used in cotton in thing.
The content of the invention
Grow cotton low temperature resistant germination authentication method and effectively evaluating method to explore one, to fast and effectively to cotton
Flower germ plasm resource carries out low temperature resistant identification, is to cultivate the low temperature resistant new varieties of cotton to lay the foundation, and the present inventor proposes simultaneously
Complete the present invention.
It is an object of the invention to provide the low temperature resistant germination authentication method of cotton.
The low temperature resistant germination authentication method of cotton of the invention, comprises the following steps：
1) selection of seed：Seed selects the seed of full seed maturation Jing after sulfuric acid lint；
2) pretreatment of seed：All seed disinfections；
3) low temperature stress is processed：Seed is placed in and is covered with the culture dish of the high-temperature sterilization of filter paper, added appropriate pure
Water, culture dish is placed on into temperature setting carries out light culture for 15 DEG C of growth cabinets；
4) low temperature resistant classification：Adopt relative germination rate the low temperature resistant level of cotton is evaluated for index, determine that its is resistance to low
The level of temperature.
Specifically, the method for specific embodiment of the invention, including：(1) selection of seed：Cultivated cotton seed
Plant after cotton ginning on shell and still have a short flannel, whether seed full, whether ripe and damaged by vermin is not substantially, de- through sulfuric acid
After suede, the short flannel on seed hull removes, and selects the pitch-black full whole seed without insect pest.If cotton seeds are newly to receive then
The seed for obtaining, needs to abolish physiological dormancy, can take heat drying method, 40 DEG C, well-ventilated, baking oven heating, drying 1 day.
(2) pretreatment of seed：Seed can kill the microorganism that a part is planted on shell when sulfuric acid lint, but before Seed Treatment
Still need to further disinfection, 5% liquor natrii hypochloritis immersion 10min, with pure water rinsing clean (4-5 time).(3) it is low
Warm Stress treatment (15 DEG C)：Seed after sterilization is placed in and is lined with the culture dish of 2 metafiltration paper that (filter paper and culture dish have been taken
High-temperature sterilization process), seed is preferably put according to a direction, keeping at a certain distance away between seed, adds in each culture dish
Enter appropriate distilled water, the filter paper of two-layer moistening is added a cover again in seed top.Appropriate moisture is kept during germination, moisture is excessive,
Easily cause seed decay；Hypohydration, seed cannot absorb enough moisture so as to affect seed to sprout.It is put into design temperature 15
DEG C artificial climate incubator in, controlled temperature be 30 DEG C.8h illumination daily, intensity of illumination is 13.5 μm of ol/ (㎡ * s)～
22.5μmol/(㎡*s).The close saturation of relative humidity in artificial climate incubator, continuous processing 10 days.Early stage is in order to determine
Temperature is effectively identified, if temperature is too low, all Shoot apex all can not normally be sprouted；During 15 DEG C of process, go out between material
Existing obvious difference, the germinating energy and germination percentage of different materials is significantly different；More than 20 DEG C of temperature, between material and material
Difference is little；Therefore, by groping repeatedly, determine that 15 DEG C are optimal low temperature germination identification temperature.In addition low temperature stress seed
Speed of germination can slow down, and the process time that seed is sprouted is extended to 10 days.(4) low temperature resistant horizontal mipmap：With reference to national industry
(1998) leaf Wuwei and Liu Jinding, calculate the ratio that normal germination seed accounts for total seed number to the stage division of standard salt resistance identification.
The normal substandard of planting sprouted is the twice of radicle and hypocotyl total length more than seed length, has main root and hypocotyl is disease-free,
Color is white, non-rot for normal bud.According to the relative germination rate (germination under relative germination rate=specific low temperature treatment conditions
Germination percentage * 100 under rate/controlled temperature), carry out low temperature resistant horizontal mipmap：
| Patience rank | Relative seedling ratio (%) |
| Height is anti- | ≥90.0 |
| It is anti- | 75.0～89.9 |
| It is resistance to | 50.0～74.9 |
| Sense | <50.0 |
The present invention provides a kind of new method for the low temperature resistant identification of cotton, is original creation in international and national, with following excellent
Point：
(1) the method is classified using Relative seedling ratio, gives objective appraisal index, is greatly reduced based on people
The error that sight factor is caused.
(2) the method adopts artificial climate incubator culture, and except setting different temperature, other conditions are in manual control
Under can reach it is completely the same.Error caused by environmental factor is reduced, makes result relatively reliable.
(3) the method is simple to operate, manpower and materials low cost, occupies little space, and can carry out the identification of high-volume material.
Description of the drawings
The representative material of tetra- Shoot apex of Fig. 1：Cotton (medium-height grass No. 1), Asiatic cotton (Central Asia 1), upland cotton (in 79),
Sea island cotton (sea 7124), in 15 DEG C of illumination boxs of low temperature, after processing 10 days, the normal seed sprouted.
Specific embodiment
The low temperature resistant germination authentication method of the cotton of embodiment 1 identifies the low temperature resistant level of 6 parts of cotton materials
1st, cotton material：
The Central Asia 1；TM-1, in 12, in 79；7124, PM90 of sea totally 6 parts of cotton materials
2nd, the selection of seed:
Cotton seeds after sulfuric acid lint, select in the same size, pitch-black full, the complete mature seed without insect pest.For
Guarantee to break the physiological dormancy of cotton seeds, under conditions of well-ventilated, being paved into a thin layer for planting experimentally son, 40 DEG C, plus
Heat drying 24h, to break seed dormancy.Save backup.
3rd, the sterilization of seed：
5% liquor natrii hypochloritis immersion 10min, with pure water rinsing clean (4-5 time).Bacterium, true can so be prevented
The seed that bacterium etc. causes goes rotten.
4th, low temperature stress is processed：
Different thermogrades are set：5 DEG C, 10 DEG C, 15 DEG C, 20 DEG C, 25 DEG C, 30 DEG C (controlled temperature).After sterilization
Seed is placed in and is lined with the culture dish of 2 metafiltration paper (filter paper and culture dish have taken high-temperature sterilization to process), and seed is neatly put,
Keeping at a certain distance away between seed, adds appropriate distilled water, seed top to add a cover two-layer moistening again in each culture dish
Filter paper.Appropriate moisture is kept during germination.It is respectively put into design temperature (5 DEG C, 10 DEG C, 15 DEG C, 20 DEG C, 25 DEG C, 30 DEG C)
In artificial climate incubator.8h illumination daily, intensity of illumination is 13.5 μm of ol/ (㎡ * s)～22.5 μm of ol/ (㎡ * s).Artificial gas
Wait the close saturation of relative humidity in incubator, continuous processing 10 days.Each process is repeated 3 times.
5th, result statistics and analysis：
Institute an inquiry within the 3rd day after process, under 20 DEG C and the Temperature Treatment of the above, seed starts to sprout.15 DEG C of low temperature stress
Under, seed starts to show money or valuables one carries unintentionally.Under the conditions of 5 DEG C and 10 DEG C, seed is not sprouted.After processing 10 days, though 5 DEG C and 10 DEG C of seeds have minority
It was observed that sprout show money or valuables one carries unintentionally, but radicle does not extend, and not very normally germinates.15 DEG C, 20 DEG C, 25 DEG C, seed is all under the conditions of 30 DEG C
Complete to sprout.The normal germinative number of the seed under statistics different temperatures, calculates Relative seedling ratio, then basis by formula
Grade scale is classified.As a result find, the germination percentage of the different cultivars of cotton lowers as temperature is reduced, at 10 DEG C and with
Under temperature, it is impossible to it is normal to sprout；Germination percentage at 15 DEG C between different materials reaches the pole level of signifiance, and 15 DEG C can conduct
The effective temperature of cotton cotton low temperature stress.20 DEG C and the temperature of the above, result is slightly with (30 DEG C) are compareed for the germination rate of identical material
It is micro- to have difference, but the difference that difference is not reaching between the level of signifiance, and material is little, it is impossible to effectively between material
Low temperature resistant level differentiated.
5th, low temperature resistant horizontal mipmap：
According to the standard of the low temperature resistant germination identification method of cotton, Relative seedling ratio is calculated by formula, then according to classification mark
Standard is classified (be shown in Table 1).
The low temperature resistant level of 16 cotton materials of table
As shown in figure 1, the representative material of four Shoot apex：Cotton (medium-height grass No. 1), Asiatic cotton (Central Asia 1), upland cotton
(in 79), sea island cotton (sea 7124), in 15 DEG C of illumination boxs of low temperature, after processing 10 days, the normal seed sprouted.
Claims (3)
1. the low temperature resistant germination authentication method of cotton, it is characterised in that methods described includes step：
(1) selection of seed, selects the whole seed without insect pest；
(2) pretreatment of seed：Disinfection seed；
(3) low temperature stress is processed：Seed after sterilization is placed in into moist environment, in 15 DEG C of Stress treatment continuous processings 10 days；
(4) low temperature resistant horizontal mipmap.
2. the low temperature resistant germination authentication method of cotton according to claim 1, it is characterised in that in step (1), for working as
The seed that year newly harvests, needs to abolish physiological dormancy, takes heat drying method, 40 DEG C, divulges information, baking oven heating, drying 1 day.
3. the low temperature resistant germination authentication method of cotton according to claim 1, it is characterised in that in step (2), with 5%
Liquor natrii hypochloritis immersion seed 10min, with pure water rinsing 4-5 time.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610267765 | 2016-04-27 | ||
| CN2016102677650 | 2016-04-27 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106613413A true CN106613413A (en) | 2017-05-10 |
Family
ID=58852827
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610604492.4A Pending CN106613413A (en) | 2016-04-27 | 2016-07-28 | Cotton low-temperature-resistant sprouting identification method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106613413A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107136418A (en) * | 2017-05-31 | 2017-09-08 | 福建农林大学 | A kind of production technology of Job's tears seedling powder |
| CN108782230A (en) * | 2018-01-08 | 2018-11-13 | 河南科技学院 | A kind of breeding method of cold-resistant cotton |
| CN110226382A (en) * | 2019-05-20 | 2019-09-13 | 湖北省农业科学院经济作物研究所 | Nature seawater coerces cotton seed germination phase Tolerant salt method |
| CN110249951A (en) * | 2019-07-25 | 2019-09-20 | 辽宁省沙地治理与利用研究所 | The freeze proof germplasm identification method of peanut |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103843491A (en) * | 2014-03-25 | 2014-06-11 | 山西省农业科学院棉花研究所 | Method for quickly detecting salt tolerance of cotton variety |
| CN105359815A (en) * | 2015-12-15 | 2016-03-02 | 中国农业科学院棉花研究所 | Method for identifying cold resistance of cotton in germination period |
-
2016
- 2016-07-28 CN CN201610604492.4A patent/CN106613413A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103843491A (en) * | 2014-03-25 | 2014-06-11 | 山西省农业科学院棉花研究所 | Method for quickly detecting salt tolerance of cotton variety |
| CN105359815A (en) * | 2015-12-15 | 2016-03-02 | 中国农业科学院棉花研究所 | Method for identifying cold resistance of cotton in germination period |
Non-Patent Citations (2)
| Title |
|---|
| 王俊娟等: "棉花萌发期抗冷性鉴定", 《中国棉花学会2007年年会论文汇编》 * |
| 赵俊丽等: "棉花萌发期耐低温性鉴定方法的研究", 《中国棉花学会2007年年会论文汇编》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107136418A (en) * | 2017-05-31 | 2017-09-08 | 福建农林大学 | A kind of production technology of Job's tears seedling powder |
| CN108782230A (en) * | 2018-01-08 | 2018-11-13 | 河南科技学院 | A kind of breeding method of cold-resistant cotton |
| CN110226382A (en) * | 2019-05-20 | 2019-09-13 | 湖北省农业科学院经济作物研究所 | Nature seawater coerces cotton seed germination phase Tolerant salt method |
| CN110249951A (en) * | 2019-07-25 | 2019-09-20 | 辽宁省沙地治理与利用研究所 | The freeze proof germplasm identification method of peanut |
| CN110249951B (en) * | 2019-07-25 | 2021-06-11 | 辽宁省沙地治理与利用研究所 | Anti-freezing peanut germplasm identification method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105519367B (en) | Wild cotton seedling stage low temperature resistant identification method | |
| CN105850622B (en) | A kind of high temperature leads to the identification method of rice grain infertility | |
| CN106613288B (en) | Rapid identification method for resistance of broad beans to pea aphids | |
| CN106613413A (en) | Cotton low-temperature-resistant sprouting identification method | |
| CN101897253A (en) | Method for identifying seedling stage stress resistance of tobacco variety | |
| CN107896981B (en) | Breeding method of genetically stable leaf etiolated watermelon variety | |
| CN109496813A (en) | A kind of indoor ciltivating process of heartsease, angle violet class flowers | |
| CN110235639A (en) | Nature seawater coerces cotton in seedling stage Tolerant salt method | |
| CN110226382A (en) | Nature seawater coerces cotton seed germination phase Tolerant salt method | |
| CN113287513A (en) | Eggplant haploid plant sexual propagation doubling method based on improvement of pollen activity | |
| CN103210839B (en) | Method for improving sclerotiniose resistance of oilseed rapes | |
| CN107190049B (en) | Method for identifying or screening disease-resistant rice material by inducing rice blast seedling plague through field natural inoculation | |
| CN109750080B (en) | Method for rapidly screening peanut fruit rot resistant germplasm resources | |
| CN107750509A (en) | Barley moisture-proof simple Identification method | |
| CN107787698A (en) | A kind of production method for the seed potato for avoiding soil-borne disease from infecting | |
| You et al. | Relationships between temperature-light meteorological factors and seedcotton biomass per boll at different boll positions | |
| CN109988815A (en) | A method of sharp eyespot resistance is identified using the in vitro stalk of rice | |
| CN113331013B (en) | Method for sowing and forcing culture of lilium pinosum | |
| JP2012010652A (en) | Method for cultivating onion, and onion | |
| CN113016518B (en) | Resistance identification method for celery leaf spot | |
| CN108739127B (en) | Method for rapidly comparing high-temperature drought resistance characteristics of tomato varieties | |
| CN107018707A (en) | A kind of method for identifying rape seed Germination And Seedling low temperature tolerance characteristicses | |
| CN114199714A (en) | Method for high-pass identification and selection of plant resistant varieties by integrating physiological indexes of germination stage growth and seedling stage | |
| Ertle et al. | Watermelon Seedling Quality, Growth, and Development as Affected by Grafting and Chilling Exposure During Simulated Transportation | |
| CN107815487A (en) | A kind of method of indoor identification wild cotton seedling stage resistance to verticillium wilt |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170510 |
|
| RJ01 | Rejection of invention patent application after publication |