CN110226382A - Nature seawater coerces cotton seed germination phase Tolerant salt method - Google Patents
Nature seawater coerces cotton seed germination phase Tolerant salt method Download PDFInfo
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- CN110226382A CN110226382A CN201910416939.9A CN201910416939A CN110226382A CN 110226382 A CN110226382 A CN 110226382A CN 201910416939 A CN201910416939 A CN 201910416939A CN 110226382 A CN110226382 A CN 110226382A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
- A01C1/025—Testing seeds for determining their viability or germination capacity
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Abstract
The present invention provides a kind of nature seawater stress cotton seed germination phase Tolerant salt method, comprising the following steps: the selection of seed: seed selects the seed of full seed maturation after sulfuric acid lint；The pretreatment of seed: it carries out disinfection processing to all seeds；Nature seawater Stress treatment: Stress treatment is carried out to seed using the nature seawater that concentration is 50%；Salt tolerant classification: then the relative germination rate of the seed after calculating Stress treatment is evaluated cotton salt tolerant level using relative germination rate as index, determines that cotton salt tolerant is horizontal.Method choice nature seawater stress of the invention, not only can be by seed relative germination rate expert evidence salt tolerance after stress, but also can simulate strand beachhead and plant cotton region domain cotton seed germination environment, and screening is suitble to the cotton material of above-mentioned environment plantation.
Description
Technical field
The present invention relates to plant physiology more particularly to a kind of nature seawater to coerce cotton seed germination phase Tolerant salt side
Method.
Background technique
To guarantee grain security, China plants the whole west in cotton region and moves into east, i.e., northwestwards inland salt-soda soil and strand beachhead turn
It moves.It is saline and alkaline to have become one of the abiotic stress factor for limiting above-mentioned regional Cotton Production.Cotton is by Saline Alkali Stress in production,
It is mainly manifested in Their Seed Germinating Period.The infiltration as caused by salinity makes kind of a filial generation in Their Seed Germinating Period meeting stress-inducing seed dormancy
The activity of thanking slows down, and germination period is caused to postpone or do not germinate.Currently, being concentrated on about cotton seed germination phase Tolerant salt
Using artificial seawater or single salt stress, had not been reported using nature seawater stress identification.Cotton region is planted in strand beachhead simultaneously to use
Nature seawater pours, and is conducive to save irrigation water.Therefore, identification cotton seed germination phase salt tolerance is coerced using nature seawater
Germplasm is very necessary.
Summary of the invention
In view of this, the present invention provides a kind of nature seawaters to coerce cotton seed germination phase Tolerant salt method, to
Effectively identification quality of cotton salt-tolerant seeds lays the foundation to cultivate the cotton variety for planting cotton suitable for seawater.
The present invention provides a kind of nature seawater stress cotton seed germination phase Tolerant salt method, comprising the following steps:
S1, the selection of seed: seed selects the seed of full seed maturation after sulfuric acid lint；
The pretreatment of seed: S2 carries out disinfection processing to all seeds selected；
S3, nature seawater Stress treatment: treated that seed is coerced to disinfection for the nature seawater for being 50% using concentration
Compel processing；
S4, salt tolerant classification: the relative germination rate of the seed after calculating Stress treatment, then using relative germination rate as index pair
Cotton salt tolerant level is evaluated, and determines that cotton salt tolerant is horizontal.
Further, it in step S1, is planted after the cotton ginning of cultivated cotton seed on shell and still has a short flannel, whether seed full,
Whether mature and whether damaged by vermin is all unobvious, after persulfuric acid lint, the short flannel on seed hull removes, and selects pitch-black full
Whole seed without insect pest；If cotton seeds are the seeds newly harvested current year, need to abolish physiological dormancy, heating can be taken
Seasoning: 40 DEG C, well-ventilated, baking oven heating, drying 1 day.
Further, it in step S2, carries out disinfection the process of processing to seed are as follows: utilize 5% liquor natrii hypochloritis's leaching
10min is steeped, then clean with pure water rinsing.
Further, in step S3, seed utilizes Stress treatment process when filter paper culture are as follows: sets the seed after disinfection
In the culture dish for being lined with 2 layers of filter paper, filter paper and culture dish have taken high-temperature sterilization to handle, and seed is preferably according to a direction
It puts, it is spaced apart between seed, the nature seawater that 15mL concentration is 50%, control are added in each culture dish of test group
Pure water is added in each culture dish of group；The nature seawater that test group regular replenishment concentration is 50% during germination, control group supplement are pure
Water；All culture dishes are put into the artificial climate incubator that set temperature is 28 DEG C, daily 8h illumination, intensity of illumination 13.5
μmol/(m2S)~22.5 μm ol/ (m2S), the close saturation of the relative humidity in artificial climate incubator, continuous processing 3 days.
Further, in step S3, seed utilizes Stress treatment process when sandy soil culture are as follows: will be on culture box middle berth
3cm thickness sandy soil, sandy soil and culture box have taken high-temperature sterilization to handle, and pouring into 150mL concentration in each culture box of test group is
50% nature seawater, mixes well, and pure water is added in each culture box of control group；Seed after disinfection is placed in culture box
In, seed is preferably put according to a direction, spaced apart between seed；Test group regular replenishment concentration is during germination
50% nature seawater, control group supplement pure water；All culture boxes are put into the artificial climate incubator that set temperature is 28 DEG C
In, daily 8h illumination, intensity of illumination is 13.5 μm of ol/ (m2S)~22.5 μm ol/ (m2S), in artificial climate incubator
The close saturation of relative humidity, continuous processing 7 days.
Further, in step S5, nature seawater is calculated separately using the stage division that national sector standard salt resistance is identified
The percentage of seedgermination under the conditions of percentage of seedgermination and pure water under treatment conditions, then according to the kind under nature seawater treatment conditions
Percentage of seedgermination under the conditions of sub- germination percentage and pure water calculates relative germination rate；Wherein, percentage of seedgermination is normal germination seed
The ratio of the total seed number of Zhan, the standard of normal germination seed are twice that radicle and hypocotyl total length are greater than seed length, are had
Main root and hypocotyl is disease-free, color is white, and non-rot is normal bud；The calculation formula of relative germination rate are as follows:
Wherein, the percentage of seedgermination under nature seawater treatment conditions is that normal germination seed accounts under nature seawater treatment conditions
The ratio of total seed number；Percentage of seedgermination under the conditions of Pure water preparation is the total seed of normal germination seed Zhan under the conditions of Pure water preparation
Several ratios；
Cotton salt tolerant level is classified using relative germination rate as index, the standard of salt tolerant horizontal mipmap are as follows: opposite hair
Value >=90.0% of bud rate, patience rank are highly resistance；The value of relative germination rate is 75.0%~89.9%, and patience rank is anti-；
The value of relative germination rate is 50.0%~74.9%, and patience rank is resistance to；Value < 50.0% of relative germination rate, patience rank are
Sense.
The present invention provides a kind of new method for cotton Tolerant salt, is original creation in international and national, has the advantage that
(1) present invention uses nature seawater Stress treatment, can identify that the germination period of different cotton materials is resistance in a short time
Salt facilitates the cotton salt-enduring cultivars for screening suitable seawater irrigation and the plantation of strand beachhead；
(2) present invention is classified using relative germination rate, is given objective appraisal index, is greatly reduced artificial master
Error caused by sight factor；
(3) present invention uses artificial climate incubator culture, and in addition to setting different concentration of seawater, other conditions are artificial
It can reach completely the same under control, reduce error caused by environmental factor, keep result relatively reliable；
(4) operation of the present invention is simple, and stress from outside environment is uniform, and manpower and material resources are at low cost, and occupied space is few, is conducive to standard
Really, quick and a large amount of cotton seed germination phase Salt-Tolerance Identification.
Detailed description of the invention
Fig. 1 is a kind of flow diagram of nature seawater stress cotton seed germination phase Tolerant salt method of the present invention.
Fig. 2 is the filter paper sproutung method seed sprouting situation of the embodiment of the present invention 1.
Fig. 3 is the sand culture sproutung method seed sprouting situation of the embodiment of the present invention 2.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, below in conjunction with attached drawing to embodiment party of the present invention
Formula is further described.
With reference to Fig. 1, the embodiment provides a kind of nature seawaters to coerce cotton seed germination phase Tolerant salt side
Method, comprising the following steps:
Step S1, the selection of seed: seed selects the seed of full seed maturation after sulfuric acid lint；
The pretreatment of seed: step S2 carries out disinfection processing to all seeds selected；
Step S3, nature seawater Stress treatment: using concentration be 50% nature seawater to disinfection treated seed into
Row Stress treatment；The preparation for the nature seawater that concentration is 50% are as follows: mix nature seawater and fresh water according to volume ratio 1:1
It closes, the water diverting place for the nature seawater used in the present invention is the area the Sanya Ya Zhou gulf Ya Zhou；
Salt tolerant classification: step S4 uses relative germination rate to evaluate for index cotton salt tolerant level, determines its salt tolerant
Level.
Below with reference to embodiment to nature seawater provided by the invention coerce cotton seed germination phase Tolerant salt method into
Row is described in detail.
Embodiment 1: nature seawater coerces cotton seed germination phase salt tolerant germination identification method and identifies the resistance to of 6 parts of cotton materials
Salt level
1, the present embodiment 1 use cotton material are as follows: Qiu Ke 2 (hereinafter referred to as Qiu 2), Qiu Ke 1b (hereinafter referred to as Qiu 1b),
Sharp spot cotton 25 (hereinafter referred to as point -25), Mary add Lang Temian 85 (hereinafter referred to as Ma 85), nakamise 12 (12 in hereinafter referred to as),
Upland cotton TM-1 (hereinafter referred to as TM-1).
2, the selection of seed:
Cotton seeds after sulfuric acid lint, select in the same size, and pitch-black full complete mature seed without insect pest is
The physiological dormancy for ensuring to break cotton seeds, a thin layer is paved into for planting experimentally son, 40 DEG C, adds under conditions of well-ventilated
Heat is dried for 24 hours, to break seed dormancy.It saves backup.
3, the disinfection of seed:
Seed is still needed to before seed treatment through that can kill the microorganism on a part of kind of shell when persulfuric acid lint into one
Step is disinfected: 5% liquor natrii hypochloritis impregnates 10min, with pure water rinsing 4-5 times.Bacterium, true can be prevented in this way
Seed caused by bacterium etc. goes rotten.
4, filter paper chitting piece nature seawater Stress treatment:
Different nature seawater concentration gradients is set: 0%, 25%, 50%, 100%；Wherein 0% be control group, 25%,
50%, 100% is test group；Seed after disinfection is placed in the culture dish for being lined with 2 layers of filter paper to (filter paper and culture dish have been adopted
High-temperature sterilization is taken to handle), seed is put according to a direction, and it is spaced apart between seed, in each culture dish of test group
The nature seawater of setting concentration gradient is added, pure water is added in control group；During germination test group regular replenishment corresponding concentration from
Right seawater, control group supplement pure water；Culture dish is put into the artificial climate incubator that set temperature is 28 DEG C, daily 8h light
According to intensity of illumination is 13.5 μm of ol/ (m2S)~22.5 μm ol/ (m2S), the relative humidity in artificial climate incubator is close
Saturation, continuous processing 3 days.Each processing is repeated 2 times.
5, result statistics and analysis:
It institutes an inquiry within the 1st day after processing, the seed of control group and the processing of 25% nature seawater shows money or valuables one carries unintentionally, at 50% nature seawater
The seed water suction of reason swells, control group and 25% nature seawater processing seed hypocotyl and radicle elongation after 3 days；50% is naturally extra large
The seed base-root of water process extends, and reaches 2 times of seed length；The seed of 100% nature seawater processing is without sprouting sign.Statistics
The normal germinative number of seed under various concentration calculates relative germination rate, is then classified according to grade scale.As a result it sends out
Existing, the germination percentage of the different cultivars of cotton lowers as nature seawater concentration increases, under 100% nature seawater concentration, no
It can normally sprout；Germination percentage difference when concentration of seawater is 50% between different materials is obvious, and 50% can be used as cotton nature
The effective concentration of seawater stress；25% and concentration below, the germination rate of identical material slightly have difference with (0%) result is compareed
Not, the difference but between material is little, cannot effectively identify to the salt tolerant level between material, it is thus determined that 50% is
Optimal filter paper sproutung method nature seawater stress identification concentration.
50% nature seawater handle 3 days after, filter paper sproutung method seed sprouting situation as shown in Fig. 2, figure it is seen that
The sprouting situation of -85 cotton seed of Ma is best in filter paper sproutung method.
It is classified according to cotton salt tolerant level of the grade scale to 50% nature seawater Stress treatment, classification results such as table
Shown in 1:
Table 1: the salt tolerant of 6 cotton materials is horizontal in filter paper sproutung method
Embodiment 2: nature seawater coerces cotton seed germination phase salt tolerant germination identification method and identifies the resistance to of 6 parts of cotton materials
Salt level
1, the cotton material that the present embodiment 2 uses is the same as embodiment 1.
2, the selection of seed:
Cotton seeds after sulfuric acid lint, select in the same size, and pitch-black full complete mature seed without insect pest is
The physiological dormancy for ensuring to break cotton seeds, a thin layer is paved into for planting experimentally son, 40 DEG C, adds under conditions of well-ventilated
Heat is dried for 24 hours, to break seed dormancy.It saves backup.
3, the disinfection of seed:
Seed is still needed to before seed treatment through that can kill the microorganism on a part of kind of shell when persulfuric acid lint into one
Step is disinfected: 5% liquor natrii hypochloritis impregnates 10min, with pure water rinsing 4-5 times.Bacterium, true can be prevented in this way
Seed caused by bacterium etc. goes rotten.
4, sand culture chitting piece nature seawater Stress treatment:
Different nature seawater concentration gradients is set: 0%, 25%, 50%, 100%；Wherein 0% be control group, 25%,
50%, 100% is test group；3cm thickness sandy soil on box middle berth (sandy soil and culture box have taken high-temperature sterilization to handle) will be cultivated,
Test group pours into the nature seawater of 150mL setting concentration gradient respectively, mixes well, and pure water is added in control group；After disinfection
Seed is placed in culture box；Seed is neatly put, spaced apart between seed.Test group regular replenishment is corresponding during germination
The nature seawater of concentration, control group supplement pure water；Box will be cultivated to be put into the artificial climate incubator that set temperature is 28 DEG C.Often
Its 8h illumination, intensity of illumination are 13.5 μm of ol/ (m2S)~22.5 μm ol/ (m2·s).Continuous processing 7 days.
5, result statistics and analysis:
It institutes an inquiry within the 3rd day after processing, the seed of control group and the processing of 25% nature seawater breaks ground.After 5 days control group and
25% nature seawater processing seed cotyledons are open and flat, and the seed of 50% nature seawater processing breaks ground and grows up, and cotyledon is not open and flat.
The seed of 100% nature seawater processing is without sprouting sign.
The normal germinative number for counting the seed under various concentration calculates relative germination rate by formula, then according to classification
Standard is classified.As a result, it has been found that the germination percentage of the different cultivars of cotton lowers as nature seawater concentration increases,
Under 100% nature seawater concentration, cannot normally it sprout；Germination percentage difference when concentration of seawater is 50% between different materials is bright
Aobvious, 50% can be used as the effective concentration of cotton nature seawater stress；25% and concentration below, the germination rate of identical material with
Control group (0%) result slightly has difference, but the difference between material is little, cannot be effectively horizontal to the salt tolerant between material
Identified；Accordingly, it is determined that 50% is optimal sand culture sproutung method nature seawater stress identification concentration.
50% nature seawater handle 3 days after, sand culture sproutung method seed sprouting situation as shown in figure 3, from figure 3, it can be seen that
The sprouting situation of -85 cotton seed of Ma 12 cotton seeds in is best in sand culture sproutung method.
It is classified according to cotton salt tolerant level of the grade scale to 50% nature seawater Stress treatment, classification results such as table
Shown in 2:
Table 2: the salt tolerant of 6 cotton materials is horizontal in sand culture sproutung method
| Title material | Relative germination rate (%) | Patience rank |
| Qiu 2 | 5.6 | Sense |
| Qiu 1b | 14.3 | Sense |
| Ma -85 | 94.4 | Highly resistance |
| Point -25 | 87.5 | It is anti- |
| In 12 | 94.7 | Highly resistance |
| TM-1 | 77.7 | It is anti- |
In the absence of conflict, the feature in embodiment and embodiment herein-above set forth can be combined with each other.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all in spirit of the invention and
Within principle, any modification, equivalent replacement, improvement and so on be should all be included in the protection scope of the present invention.
Claims (7)
1. a kind of nature seawater coerces cotton seed germination phase Tolerant salt method, which comprises the following steps:
S1, the selection of seed: seed selects the seed of full seed maturation after sulfuric acid lint；
The pretreatment of seed: S2 carries out disinfection processing to all seeds selected；
S3, nature seawater Stress treatment: treated that seed carries out at stress to disinfection for the nature seawater for being 50% using concentration
Reason；
S4, salt tolerant classification: the relative germination rate of the seed after calculating Stress treatment, is then index to cotton using relative germination rate
Salt tolerant level is evaluated, and determines that cotton salt tolerant is horizontal.
2. nature seawater according to claim 1 coerces cotton seed germination phase Tolerant salt method, which is characterized in that step
In rapid S2, carry out disinfection the process of processing to seed are as follows: impregnate 10min using 5% liquor natrii hypochloritis, then use pure water rinsing
Completely.
3. nature seawater according to claim 1 coerces cotton seed germination phase Tolerant salt method, which is characterized in that step
In rapid S3, seed utilizes Stress treatment process when filter paper culture are as follows: the seed after disinfection is placed in the culture dish for being lined with filter paper
In, the nature seawater that concentration is 50% is added in each culture dish of test group, each culture dish of control group is added pure water, will own
Culture dish is put into artificial climate incubator and cultivates.
4. nature seawater according to claim 1 coerces cotton seed germination phase Tolerant salt method, which is characterized in that step
In rapid S3, seed utilizes Stress treatment process when sandy soil culture are as follows: by sandy soil, each culture box of test group on culture box middle berth
In pour into the nature seawater that concentration is 50%, pure water is added in each culture box of control group；Seed after disinfection is placed in culture
In box, puts neatly, then all culture boxes are put into artificial climate incubator and are cultivated.
5. nature seawater according to claim 3 or 4 coerces cotton seed germination phase Tolerant salt method, feature exists
In the calculation formula of the relative germination rate of the seed after Stress treatment are as follows:
Wherein, the percentage of seedgermination under nature seawater treatment conditions is normal total kind of Zhan of germination seed under nature seawater treatment conditions
The ratio of subnumber；Percentage of seedgermination under the conditions of Pure water preparation is the total seed number of normal germination seed Zhan under the conditions of Pure water preparation
Ratio；The standard of normal germination seed is twice that radicle and hypocotyl total length are greater than seed length.
6. nature seawater according to claim 5 coerces cotton seed germination phase Tolerant salt method, which is characterized in that right
The grade scale that cotton salt tolerant level is evaluated are as follows: value >=90.0% of relative germination rate, patience rank are highly resistance；Opposite hair
The value of bud rate is 75.0%~89.9%, and patience rank is anti-；The value of relative germination rate is 50.0%~74.9%, patience rank
It is resistance to；Value < 50.0% of relative germination rate, patience rank are sense.
7. nature seawater according to claim 3 or 4 coerces cotton seed germination phase Tolerant salt method, feature exists
In the temperature setting of artificial climate incubator is 28 DEG C, daily 8h illumination, and intensity of illumination is 13.5 μm of ol/ (m2S)~22.5
μmol/(m2·s)。
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| CN112005657A (en) * | 2020-09-03 | 2020-12-01 | 青岛农业大学 | Method for screening salt-tolerant germplasm of quinoa by utilizing natural seawater |
| CN112868522A (en) * | 2021-01-19 | 2021-06-01 | 山西农业大学 | Method for creating new cotton germplasm by distant hybridization |
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