CN106577324B - Frightened test device and method - Google Patents
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- CN106577324B CN106577324B CN201611217295.3A CN201611217295A CN106577324B CN 106577324 B CN106577324 B CN 106577324B CN 201611217295 A CN201611217295 A CN 201611217295A CN 106577324 B CN106577324 B CN 106577324B
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- 238000012360 testing method Methods 0.000 title claims abstract description 103
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000010171 animal model Methods 0.000 claims abstract description 68
- 238000013459 approach Methods 0.000 claims abstract description 55
- 238000004088 simulation Methods 0.000 claims abstract description 14
- 206010014405 Electrocution Diseases 0.000 claims abstract description 13
- 238000010438 heat treatment Methods 0.000 claims abstract description 13
- 238000004458 analytical method Methods 0.000 claims description 22
- 230000035479 physiological effects, processes and functions Effects 0.000 claims description 22
- 230000005693 optoelectronics Effects 0.000 claims description 20
- 238000012544 monitoring process Methods 0.000 claims description 19
- 241001465754 Metazoa Species 0.000 claims description 15
- 239000013307 optical fiber Substances 0.000 claims description 13
- 238000010191 image analysis Methods 0.000 claims description 10
- 230000005611 electricity Effects 0.000 claims description 9
- 241000894007 species Species 0.000 claims description 8
- 210000004556 brain Anatomy 0.000 claims description 6
- 108090000623 proteins and genes Proteins 0.000 claims description 6
- 238000010998 test method Methods 0.000 claims description 6
- 230000003287 optical effect Effects 0.000 claims description 5
- 230000005540 biological transmission Effects 0.000 claims description 3
- 238000004891 communication Methods 0.000 claims description 3
- 238000012806 monitoring device Methods 0.000 claims 1
- 230000035807 sensation Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 6
- 206010041247 Social fear Diseases 0.000 abstract description 3
- 238000002474 experimental method Methods 0.000 description 5
- 238000010586 diagram Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 230000035939 shock Effects 0.000 description 4
- 206010016275 Fear Diseases 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 210000005036 nerve Anatomy 0.000 description 2
- 230000001537 neural effect Effects 0.000 description 2
- 230000005622 photoelectricity Effects 0.000 description 2
- 241000270295 Serpentes Species 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000004378 air conditioning Methods 0.000 description 1
- 230000001174 ascending effect Effects 0.000 description 1
- 230000007177 brain activity Effects 0.000 description 1
- 210000005013 brain tissue Anatomy 0.000 description 1
- 230000019771 cognition Effects 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
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- 230000006872 improvement Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
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- 230000036651 mood Effects 0.000 description 1
- 210000002569 neuron Anatomy 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000011514 reflex Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
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- 239000000523 sample Substances 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/03—Housing for domestic or laboratory animals
- A01K1/031—Cages for laboratory animals; Cages for measuring metabolism of animals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K29/00—Other apparatus for animal husbandry
- A01K29/005—Monitoring or measuring activity, e.g. detecting heat or mating
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Abstract
The embodiment of the invention provides a kind of frightened test device and method, device includes: to approach frightened test device, observe frightened test device and decision zone device, and the setting of decision zone device approaches between frightened test device and the frightened test device of observation described；Approaching frightened test device includes: to approach warehouse and approach the intracorporal approaching simulation device in storehouse positioned at described；The frightened test device of the observation includes: observation warehouse and electrocution device；The decision zone device includes: decision area warehouse and the heating equipment positioned at the decision area warehouse bottom, and decision area warehouse approaches warehouse and the observation warehouse is connected with described respectively.The present invention is realized to the frightened test of the instinct fear test of same experimental animal and society, simultaneously, experimental animal is observed and studied in the frightened selection between society's fear of instinct by decision zone device, the effect for improving frightened test has achieved the purpose that simultaneously to further investigate instinct fear and social fear.
Description
Technical field
The present invention relates to frightened the field of test technology, more particularly to frightened test device and method.
Background technique
Mood is an important products of brain activity, and fear is considered having animal survival in six kinds of basic emotions
Important meaning, a series of psychology and physiological reaction that animal shows when in face of dangerous can help animal quickly to flee from
Adverse environment.
Fear has the frightened difference of instinct fear and society, and instinct fear means that the fear for institute's fear object is
Do not need what consciousness participated in, just as the mankind fear snake.And the frightened cognition for then needing that it is certain to have frightened object of society
Then journey generates frightened process.
It is these two types of frightened necessarily to be generated by different neuromechanisms, but existing experimental provision can not by the two into
Row further investigation.
Summary of the invention
The embodiment of the present invention is designed to provide a kind of frightened test device and method, with frightened to instinct simultaneously and society
Meeting fear is furtherd investigate.Specific technical solution is as follows:
A kind of fear test device, comprising: it approaches frightened test device, observe frightened test device and decision zone device,
The decision zone device setting approaches between frightened test device and the frightened test device of observation described；
It is described that approach frightened test device include: to approach warehouse and approach the intracorporal approaching simulation device in storehouse positioned at described；
The frightened test device of the observation includes: observation warehouse and electrocution device；
The decision zone device includes:
Decision area warehouse and heating equipment positioned at the decision area warehouse bottom, decision area warehouse respectively with it is described
It approaches warehouse and the observation warehouse is connected.
Optionally, described device further include:
Video monitoring apparatus, the video monitoring apparatus acquisition experimental animal approach frightened test device, observation described
Frightened test device and image in decision zone device simultaneously analyze acquired image, obtain image analysis result.
Optionally, described device further include:
Physiology signal acquisition device, the physiological signal of physiology signal acquisition device acquisition experimental animal and to being acquired
Physiological signal analyzed, obtain physiological signal analysis result.
Optionally, described device further include: light regulation device,
The smooth regulation device includes: laser, waveform generator, optical fiber and implantable fine tuning optoelectronic pole, the wave
Shape generator is connect with laser, and the laser output of the laser finely tunes photoelectricity by the optical fiber and the implantable
The big intracerebral of experimental animal for being transferred to light sensitive gene is arranged in pole connection, the implantable fine tuning optoelectronic pole.
Optionally, described device further include: electrical signal collection device,
The electrical signal collection device includes: microelectrode and electrical signal collection device, and the microelectrode setting is being tested
In animal brain, the electrical signal collection device and the microelectrode are communicated to connect, and receive the telecommunications of the microelectrode transmission
Number and the electric signal is analyzed, obtain electric signal analysis result.
It optionally, include: video monitoring apparatus, physiology signal acquisition device, light regulation device and telecommunications in described device
In the case where number acquisition device, described device further include: analytical equipment,
The analytical equipment respectively with the video monitoring apparatus, physiology signal acquisition device, light regulation device and telecommunications
The communication connection of number acquisition device, the image obtained according to optical signal, the video monitoring apparatus that the smooth regulation device generates
The physiological signal that analysis result, the physiology signal acquisition device obtain analyzes result, the electrical signal collection device obtains
Electric signal is analyzed as a result, obtaining frightened test result.
Optionally, the junction for approaching warehouse and decision area warehouse is provided with the first dodge gate；The observation
The junction of warehouse and decision area warehouse is provided with the second dodge gate.
Optionally, further includes:
Be arranged in it is described approach warehouse, the observation warehouse and brightness regulation equipment in decision area warehouse and/or
Temperature control equipment.
A kind of fear test method carries out frightened test, the side to experimental animal using above-mentioned frightened test device
Method includes:
Control experimental animal approaches in warehouse described in being located at, and plays natural enemy by the approaching simulation device and approaches image；
The experimental animal is controlled to be located in the observation warehouse, by the electrocution device to the species of the experimental animal into
Row electric shock；
It controls the experimental animal to be located in decision area warehouse, opens the heating equipment to improve the decision area
The temperature of warehouse bottom.
Optionally, the frightened test device further include: light regulation device,
The smooth regulation device includes: laser, waveform generator, optical fiber and implantable fine tuning optoelectronic pole, the wave
Shape generator is connect with laser, and the laser output of the laser finely tunes photoelectricity by the optical fiber and the implantable
The big intracerebral of experimental animal for being transferred to light sensitive gene is arranged in pole connection, the implantable fine tuning optoelectronic pole；
The method also includes: the laser sending laser is controlled by waveform generator and is passed to the experiment and is moved
The big intracerebral of object.
A kind of frightened test device provided in an embodiment of the present invention and method, device include: to approach frightened test device, sight
Frightened test device and decision zone device are examined, the decision zone device setting approaches frightened test device and observation fear described
Between test device；It is described that approach frightened test device include: to approach warehouse and approach the intracorporal approaching simulation in storehouse positioned at described
Device；The frightened test device of the observation includes: observation warehouse and electrocution device；The decision zone device includes: decision area storehouse
Body and heating equipment positioned at the decision area warehouse bottom, decision area warehouse approach warehouse and the sight with described respectively
Warehouse is examined to be connected.The present invention is realized to the frightened test of the instinct fear test of same experimental animal and society, meanwhile, pass through
Decision zone device is observed and is studied in the frightened selection between society's fear of instinct to experimental animal, and frightened survey is improved
The effect of examination has achieved the purpose that simultaneously to further investigate instinct fear and social fear.
Certainly, it implements any of the products of the present invention or method must be not necessarily required to reach all the above excellent simultaneously
Point.
Detailed description of the invention
In order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, to embodiment or will show below
There is attached drawing needed in technical description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention for those of ordinary skill in the art without creative efforts, can be with
It obtains other drawings based on these drawings.
Fig. 1 is a kind of structural schematic diagram of frightened test device provided in an embodiment of the present invention；
Fig. 2 is the structural schematic diagram of another frightened test device provided in an embodiment of the present invention；
Fig. 3 is the structural schematic diagram of another frightened test device provided in an embodiment of the present invention；
Fig. 4 is the structural schematic diagram of another frightened test device provided in an embodiment of the present invention；
Fig. 5 is a kind of flow chart of frightened test method provided in an embodiment of the present invention.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
As shown in Figure 1, a kind of frightened test device provided in an embodiment of the present invention, may include: to approach frightened test dress
It sets 100, observe frightened test device 200 and decision zone device 300,
The decision zone device 300 setting it is described approach frightened test device 100 and observe frightened test device 200 it
Between；
It is described that approach frightened test device 100 include: to approach warehouse and approach storehouse intracorporal approaching simulation dress positioned at described
It sets；
The frightened test device 200 of the observation includes: observation warehouse and electrocution device；
The decision zone device 300 includes: decision area warehouse and the heating equipment positioned at the decision area warehouse bottom, institute
It states decision area warehouse and approaches warehouse and the observation warehouse is connected with described respectively.
Specifically, warehouse, observation warehouse and the decision area warehouse of approaching can be for all-transparent warehouse or with clear area
The warehouse in domain.
Specifically, warehouse, observation warehouse and the decision area warehouse of approaching can be behaviouristics cabinet, behaviouristics cabinet is
The square box of 20x20x40cm.Described approach between warehouse, observation warehouse and decision area warehouse can mutually be isolated sound
And taste.
It can be directly connected to, can also be connected by channel between each warehouse, the present invention is it is not limited here.On each warehouse
Need to be arranged corresponding hole so that experimental animal can the into/out warehouse.
Optionally, it is also provided with brightness regulation equipment and/or temperature control equipment in each warehouse, to adjust each warehouse
Interior brightness and/or temperature.Wherein, brightness regulation equipment can be intensity adjustable lighting apparatus, and temperature control equipment can
Think air-conditioning.
Wherein, the junction for approaching warehouse and decision area warehouse is provided with the first dodge gate；The observation storehouse
The junction of body and decision area warehouse is provided with the second dodge gate.Control can be facilitated to test by the two dodge gates
Animal is located in any one in above three warehouse.
Wherein, approaching simulation device can be projection device or display equipment, and it is dynamic that approaching simulation device can play experiment
The video that object natural enemy approaches, can also play animation that simulated experiment animal natural enemy approaches (such as: it is ascending to play shadow
Animation).
It is dynamic that the scene reaction that animal is captured in field by the natural enemy from high-altitude can be simulated by approaching simulation device
The instinct of object is frightened.
Wherein, experimental animal can be mouse.
Specifically, electricity can be carried out by species of the electrocution device in observation warehouse to the experimental animal
It hits, in this way, the similar situation being clicked will be closely experienced when experimental animal is located in observation warehouse, to make anti-
It answers.
Specifically, can also have animal restraint device in observation warehouse, to carry out electricity in the species to experimental animal
The species are fettered when hitting.
Wherein, electrocution device can there are many shock by electricity mode (such as different electric shock durations and intensity) to the same of experimental animal
Class animal is shocked by electricity, and in practical applications, the table of experimental animal under different electric shock modes is studied by different electric shock modes
It is existing.
Observe frightened test device 200 can make animal observe a series of similar frightened performance received after electric shocks to
It is frightened to obtain society.
The invention enables both frightened fears of instinct fear and society to carry out in the same frightened test device, and
Same animal can be stimulated, obtain response difference of two kinds of fears in same animal.
Since decision area warehouse bottom is provided with heating equipment, when heating apparatus temperature increases to a certain extent,
Experimental animal will not suffer from, it is necessary to leave decision area warehouse and experimental animal can only select to enter to approach warehouse or enter and observe
Warehouse, namely: experimental animal will select in and society's fear frightened in instinct.Selection in this way is dynamic to analyze experiment
Object is for both frightened individual choice differences.
It can only be selected under two kinds of frightened normal forms, when into when approaching warehouse, experimental animal will experience instinct and fear
Fear therefore can probe into two kinds of frightened differences of animal.
In practical applications, experimental animal can be controlled first to approach in warehouse positioned at described, pass through the approaching simulation
Device plays natural enemy and approaches image；Then it controls the experimental animal to be located in the observation warehouse, passes through the electrocution device
It shocks by electricity to the species of the experimental animal；Then it controls the experimental animal to be located in decision area warehouse, open
The heating equipment is opened to improve the temperature of the decision area warehouse bottom.
In this way, allow for experimental animal pass through first into approach warehouse and observation warehouse know corresponding bin
Intracorporal fear content, to be selected before leaving decision area warehouse.Certainly, in other embodiments of the present invention, may be used
With control first experimental animal be located at observation warehouse in, then control again experimental animal be located at approach in warehouse.
It include: to approach frightened test device, the frightened test of observation the embodiment of the invention provides a kind of frightened test device
Device and decision zone device, the setting of decision zone device approach between frightened test device and the frightened test device of observation described；
Approaching frightened test device includes: to approach warehouse and approach the intracorporal approaching simulation device in storehouse positioned at described；The observation is frightened
Test device includes: observation warehouse and electrocution device；The decision zone device include: decision area warehouse and be located at the decision area
The heating equipment of warehouse bottom, decision area warehouse approach warehouse and the observation warehouse is connected with described respectively.This hair
It is bright to realize to the frightened test of the instinct fear test of same experimental animal and society,
Meanwhile by decision zone device to experimental animal the frightened selection between society's fear of instinct carry out observation and
Research improves the effect of frightened test, has achieved the purpose that simultaneously to further investigate instinct fear and social fear.
As shown in Fig. 2, another frightened test device provided in an embodiment of the present invention, can also include: video monitoring dress
Set 400,
The video monitoring apparatus 400 acquires experimental animal and approaches frightened test device 100, the frightened test of observation described
Device 200 and image in decision zone device 300 simultaneously analyze acquired image, obtain image analysis result.
Wherein, video monitoring apparatus 400 can be made of one or more cameras and video analysis device.
It is analyzed in the image that video analysis device can acquire camera, obtains image analysis result.
Wherein, the performance of experimental animal can there are many, such as: residence time, the time for frightened behavior occur, frightened behavior
Duration, frightened behavior expression form etc..
Wherein, frightened behavior expression form can there are many, such as: freeze, escape, jump.
Another frightened test device provided in an embodiment of the present invention, can also include: physiology signal acquisition device,
The physiological signal of the physiology signal acquisition device acquisition experimental animal simultaneously divides physiological signal collected
Analysis obtains physiological signal analysis result.
Wherein, the physiology signal acquisition device is heart rate collector, and the physiological signal is heart rate.
The heart rate collector can be made of acquisition necklace and heart rate analysis equipment, and acquisition necklace is worn on experimental animal body
It on body and sends heart rate analysis equipment for heart rate signal by wired or wireless way to analyze, thus according to heart rate signal
Assess the frightened degree of experimental animal.
As shown in figure 3, another frightened test device provided in an embodiment of the present invention, can also include: light regulation device
500,
The smooth regulation device 500 may include: that laser, waveform generator, optical fiber and implantable finely tune optoelectronic pole,
The waveform generator is connect with laser, the laser output of the laser by the optical fiber and it is described can
Implanted finely tunes optoelectronic pole connection, and the experimental animal brain for being transferred to light sensitive gene is arranged in the implantable fine tuning optoelectronic pole
It is interior.
Light regulation device 500 can stimulate experimental animal brain by different stimulus modalities.
Specifically, can use waveform generator adjusts power, frequency, intensity etc. that laser projects laser.Specifically,
Laser is passed to implantable fine tuning optoelectronic pole by optical fiber to stimulate the target area of brain tissue to carry out the irradiation of specific neuronal member, comes
It realizes and a certain particular core group and neural circuitry is regulated and controled.Meanwhile waveform generator can also be by controlling laser
To block or reinforce the activity of neuron.
Another frightened test device provided in an embodiment of the present invention, can also include: electrical signal collection device,
The electrical signal collection device includes: microelectrode and electrical signal collection device, and the microelectrode setting is being tested
In animal brain, the electrical signal collection device and the microelectrode are communicated to connect, and receive the telecommunications of the microelectrode transmission
Number and the electric signal is analyzed, obtain electric signal analysis result.
Electrical signal collection device can be inserted into specific core group or brain area by microelectrode to record target nerve member
Active state, obtain target nerve member activity observation sex dread obtain behavior between correlation.
As shown in figure 4, another frightened test device provided in an embodiment of the present invention, is including: video monitoring apparatus
400, in the case where physiology signal acquisition device, light regulation device 500 and electrical signal collection device, described device further include: point
Analysis apparatus 800,
The analytical equipment 800 respectively with the video monitoring apparatus 400, physiology signal acquisition device, light regulation device
500 and electrical signal collection device communication connection, the optical signal generated according to the smooth regulation device 500, the video monitoring fill
Set physiological signal analysis result, the electric signal that image analysis result, the physiology signal acquisition device that 400 obtain obtain
The electric signal that acquisition device obtains is analyzed as a result, obtaining frightened test result.
Optical signal, the video monitoring that analytical equipment 800 of the invention can be generated according to the smooth regulation device 500
Physiological signal the analysis result, the telecommunications that image analysis result that device 400 obtains, the physiology signal acquisition device obtain
The electric signal that number acquisition device obtains is analyzed as a result, frightened test result is obtained, due to having comprehensively considered image analysis result, life
Manage the optical signal that signal analysis result, electric signal analysis result and light regulation device 500 generate, therefore the frightened test obtained
As a result more comprehensive and accurate, reliability is higher, can be with the fear performance of multi-analysis experimental animal.
Correspondingly, carrying out frightened test to experimental animal using above-mentioned test device of fearing the present invention also provides a kind of
Frightened test method.
As shown in figure 5, a kind of frightened test method provided in an embodiment of the present invention, may include:
S100, control experimental animal approach in warehouse described in being located at, and play natural enemy by the approaching simulation device and approach
Image；
S200, the control experimental animal are located in the observation warehouse, dynamic to the experiment by the electrocution device
The species of object shock by electricity；
Wherein, in other embodiments of the present invention, step S100 can also be executed after step S200.
S300, the control experimental animal are located in decision area warehouse, and it is described to improve to open the heating equipment
The temperature of decision area warehouse bottom.
Optionally, the frightened test device can also include: light regulation device,
The smooth regulation device includes: laser, waveform generator, optical fiber and implantable fine tuning optoelectronic pole, the wave
Shape generator is connect with laser,
The laser output of the laser is connect by the optical fiber and the implantable fine tuning optoelectronic pole, it is described can
The big intracerebral of experimental animal for being transferred to light sensitive gene is arranged in implanted fine tuning optoelectronic pole；
On this basis, method shown in Fig. 5 can also include: and control the laser by waveform generator to issue laser
And it is passed to the big intracerebral of the experimental animal.
The embodiment of the invention provides a kind of frightened test method, control experimental animal approaches in warehouse positioned at described, leads to
It crosses the approaching simulation device broadcasting natural enemy and approaches image；It controls the experimental animal to be located in the observation warehouse, passes through institute
Electrocution device is stated to shock by electricity to the species of the experimental animal；It controls the experimental animal and is located at decision area warehouse
In, the heating equipment is opened to improve the temperature of the decision area warehouse bottom.The present invention is realized to same experimental animal
The frightened test of the test of instinct fear and society, meanwhile, experimental animal is feared in instinct fear and society by decision zone device
Selection between fearing is observed and is studied, and is improved the effect of frightened test, has been reached while having feared to instinct fear and society
Fear the purpose furtherd investigate.
It should be noted that, in this document, relational terms such as first and second and the like are used merely to a reality
Body or operation are distinguished with another entity or operation, are deposited without necessarily requiring or implying between these entities or operation
In any actual relationship or order or sequence.Moreover, the terms "include", "comprise" or its any other variant are intended to
Non-exclusive inclusion, so that the process, method, article or equipment including a series of elements is not only wanted including those
Element, but also including other elements that are not explicitly listed, or further include for this process, method, article or equipment
Intrinsic element.In the absence of more restrictions, the element limited by sentence "including a ...", it is not excluded that
There is also other identical elements in process, method, article or equipment including the element.
Each embodiment in this specification is all made of relevant mode and describes, same and similar portion between each embodiment
Dividing may refer to each other, and each embodiment focuses on the differences from other embodiments.Especially for system reality
For applying example, since it is substantially similar to the method embodiment, so being described relatively simple, related place is referring to embodiment of the method
Part explanation.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the scope of the present invention.It is all this
Any modification, equivalent replacement, improvement and so within the spirit and principle of invention, are included within the scope of protection of the present invention.
Claims (10)
1. a kind of fear test device characterized by comprising approach frightened test device, observe frightened test device and determine
Plan zone device,
The decision zone device setting approaches between frightened test device and the frightened test device of observation described；
It is described that approach frightened test device include: to approach warehouse and approach the intracorporal approaching simulation device in storehouse positioned at described；
The frightened test device of the observation includes: observation warehouse and electrocution device；
The decision zone device includes:
Decision area warehouse and heating equipment positioned at the decision area warehouse bottom, decision area warehouse are approached with described respectively
Warehouse and the observation warehouse are connected.
2. the apparatus according to claim 1, which is characterized in that the fear test device further include:
Video monitoring apparatus, the video monitoring apparatus acquisition experimental animal approach frightened test device, observation fear described
Test device and image in decision zone device simultaneously analyze acquired image, obtain image analysis result.
3. device according to claim 1 or 2, which is characterized in that the fear test device further include:
Physiology signal acquisition device, the physiology signal acquisition device acquire the physiological signal of experimental animal and to lifes collected
Reason signal is analyzed, and physiological signal analysis result is obtained.
4. the apparatus according to claim 1, which is characterized in that
The fear test device further include: light regulation device, the smooth regulation device include: laser, waveform generator, light
Fine and implantable finely tunes optoelectronic pole, and the waveform generator is connect with laser, and the laser output of the laser passes through
The optical fiber is connect with implantable fine tuning optoelectronic pole, and the implantable fine tuning optoelectronic pole setting is being transferred to light sensation base
The big intracerebral of the experimental animal of cause；
Alternatively,
It is described fear test device further include the light tune control device and be two following at least one of: video monitoring
Device and physiology signal acquisition device；Wherein, the video monitoring apparatus acquisition experimental animal approaches frightened test dress described
It sets, observe frightened test device and image in decision zone device and acquired image is analyzed, obtain image analysis
As a result；The physiological signal of the physiology signal acquisition device acquisition experimental animal simultaneously analyzes physiological signal collected,
Obtain physiological signal analysis result.
5. the apparatus according to claim 1, which is characterized in that
The fear test device further include: electrical signal collection device, the electrical signal collection device include: microelectrode and electricity
Signal picker, in the big intracerebral of experimental animal, the electrical signal collection device and the microelectrode are logical for the microelectrode setting
Letter connection receives the electric signal of the microelectrode transmission and analyzes the electric signal, obtains electric signal analysis result；
Alternatively,
It is described fear test device further include the electrical signal collection device and be three following at least one of: video prison
Control device, physiology signal acquisition device and light regulation device；Wherein, the video monitoring apparatus acquisition experimental animal is forced described
Nearly fear test device is observed frightened test device and image in decision zone device and is analyzed acquired image,
Obtain image analysis result；The physiological signal of the physiology signal acquisition device acquisition experimental animal simultaneously believes physiology collected
It number is analyzed, obtains physiological signal analysis result；The smooth regulation device includes: laser, waveform generator, optical fiber and can
Implanted finely tunes optoelectronic pole, and the waveform generator is connect with laser, and the laser output of the laser passes through the light
Fine to connect with implantable fine tuning optoelectronic pole, the reality for being transferred to light sensitive gene is arranged in the implantable fine tuning optoelectronic pole
It tests in animal brain.
6. device according to claim 5, which is characterized in that the frightened test device include video monitoring apparatus,
In the case where physiology signal acquisition device, light regulation device and electrical signal collection device, the fear test device further include: point
Analysis apparatus,
The analytical equipment is adopted with the video monitoring apparatus, physiology signal acquisition device, light regulation device and electric signal respectively
Acquisition means communication connection, the image analysis obtained according to optical signal, the video monitoring apparatus that the smooth regulation device generates
As a result, the telecommunications that the physiological signal that the physiology signal acquisition device obtains analyzes result, the electrical signal collection device obtains
Number analysis as a result, obtaining frightened test result.
7. the apparatus according to claim 1, which is characterized in that the junction for approaching warehouse Yu decision area warehouse
It is provided with the first dodge gate；The junction of the observation warehouse and decision area warehouse is provided with the second dodge gate.
8. the apparatus according to claim 1, which is characterized in that further include:
It is arranged in and described approaches warehouse, the observation warehouse and brightness regulation equipment and/or temperature in decision area warehouse
Adjustment equipment.
9. it is a kind of fear test method, which is characterized in that using frightened test device described in claim 1 to experimental animal into
The frightened test of row, which comprises
Control experimental animal approaches in warehouse described in being located at, and plays natural enemy by the approaching simulation device and approaches image；Control
The experimental animal is located in the observation warehouse, carries out electricity by species of the electrocution device to the experimental animal
It hits；
It controls the experimental animal to be located in decision area warehouse, opens the heating equipment to improve decision area warehouse
The temperature of bottom.
10. according to the method described in claim 9, it is characterized in that, the fear test device further include: light regulation device,
The smooth regulation device includes: laser, waveform generator, optical fiber and implantable fine tuning optoelectronic pole, the waveform hair
Raw device is connect with laser, and the laser output of the laser is finely tuned optoelectronic pole by the optical fiber and the implantable and connected
It connects, the big intracerebral of experimental animal for being transferred to light sensitive gene is arranged in the implantable fine tuning optoelectronic pole；
The method also includes: the laser sending laser is controlled by waveform generator and is passed to the experimental animal
Big intracerebral.
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