CN1065751C - Ledebouriella polysaccharide and its preparation - Google Patents
Ledebouriella polysaccharide and its preparation Download PDFInfo
- Publication number
- CN1065751C CN1065751C CN95104920A CN95104920A CN1065751C CN 1065751 C CN1065751 C CN 1065751C CN 95104920 A CN95104920 A CN 95104920A CN 95104920 A CN95104920 A CN 95104920A CN 1065751 C CN1065751 C CN 1065751C
- Authority
- CN
- China
- Prior art keywords
- preparation
- sposknikovan
- polysaccharide
- injection
- radix saposhnikoviae
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention relates to a method for extracting polysaccharide from ledebouriella root (traditional Chinese medicine), and a preparation method of a polysaccharide preparation. The purpose of the present invention is to extract a new variety for preventing and treating AIDS from traditional Chinese medicines. The polysaccharide is extracted from ledebouriella root (traditional Chinese medicine), and belongs to a water-soluble substance; the experiment proves that the polysaccharide has the functions of enhancing immunity, resisting tumors and resisting AIDS viruses. The purpose of the present invention is also to provide a ledebouriella root polysaccharide preparing technology which has the advantages of high yield, low cost and simple and convenient technology and is suitable for industrial production, and the ledebouriella root polysaccharide can also be prepared into dosage forms with high medicinal effect and strong stability.
Description
The present invention relates to the preparation method of a kind of Chinese medicine sposknikovan and preparation thereof, belong to field of medicaments.
In recent years, the AIDS that spreads is to the mankind's threat at a high speed, make and seek the active drug prevent and treat AIDS and become current China and even in the world and urgent work, especially from aboundresources, seek in the little Chinese medicine of toxic and side effects and prevent and treat the active drug of AIDS, be subjected to the great attention of Chinese scholars.The Chinese medicine Radix Saposhnikoviae is the dry root of Saposhnilcovia divaracata (Turcz.) Schischk, the traditional Chinese medical science is used for the diseases caused by exogenous pathogenic factor exterior syndrome more, rheumatic arthralgia, when the inventor prevents and treats AIDS Chinese medicine in screening, from the Chinese medicine Radix Saposhnikoviae, extract the preparation polysaccharide, through carrying out inside and outside immunomodulating, antitumor test and the test of AIDS resisting poison repeatedly, prove conclusively its drug action.Selected Radix Saposhnikoviae is the certified products Radix Saposhnikoviae, comprises RADIX SAPOSHINOKOVIAE from Northeast, mouthful Radix Saposhnikoviae, the mountain Radix Saposhnikoviae.
The objective of the invention is to from Chinese medicine, extract new varieties, the preparation method of a kind of Chinese medicine sposknikovan and preparation thereof is provided with the AIDS of preventing and treating.Polysaccharide is the water-soluble substances that extracts from the Chinese medicine Radix Saposhnikoviae, proves that by effect experiment it has immunostimulant, antitumor and AIDS resisting toxic action.Another object of the present invention is to seek a kind of yield height, and cost is low.The suitable industrial sposknikovan preparation technology that technology is easy.The 3rd purpose of invention is that sposknikovan is made the drug effect height, stable living high dosage form.
Realize that concrete technical scheme of the present invention is:
1, the preparation method of sposknikovan: the Chinese medicine Radix Saposhnikoviae is pulverized, lower alcohol (can be methanol, ethanol and the propanol more than 75%) defat with high concentration, the defat medical material is air-dry to nothing alcohol flavor, available following two kinds of methods are handled: a, medical material put water decocts in the decoction pot, behind the elimination leach cooking liquid, medicinal residues steep three times with alkali, and solid-to-liquid ratio is 1: 6-12, the time is 0.5-2 hour.B, with medical material directly with alkali liquor dipping three times, merge three immersion.Hydroxide, carbonate, acetate and ammonium hydroxide that wherein used alkali can be monovalent metallic ion.
Above-mentioned immersion is neutralized to PH neutrality with 36% acetic acid and glacial acetic acid, is concentrated in water-bath below 60 ℃ to be rare flowing soaking paste, and relative density is 1.01-1.05, put in the centrifuge (3000 rev/mins) centrifugal 10-20 minute, discard precipitate, supernatant adds lower alcohol and reaches more than 80%, after fully stirring, cold preservation 8-36 hour, siphon goes out supernatant, and is centrifugal, drying precipitate, the hermetic container of packing into is preserved, and is standby.
Dried precipitate is made into aqueous solution, dialyses and ultra-filtration, and the filter membrane specification is 60,000 molecular weight.The circulation dialysis time is 16-72 hour, collects circulation fluid, concentrates in water-bath below 60 ℃, add lower alcohol and reach more than 80%, and cold preservation, centrifugal, drying gets the sposknikovan dry product.
Sposknikovan with the present invention's preparation is that taupe does not have the type powder, and polyoses content 〉=30% (in galacturonic acid) given birth in its acid; Protein content 2-3% (in calf serum).2, the preparation method of preparation;
Get sposknikovan 10 gram, add injection water 1000ml or be mixed with aqueous solution in the ratio of 2-10mg/ml, transfer PH to 7.5-9 with 1% sodium hydroxide again, best pH value is 8, and heating for dissolving is filtered.Filtrate is through heat treated, and cold preservation is once filtered, and adds water for injection to recipe quantity, fine straining, fill, sealing by fusing, lamp inspection, lettering, packing.
The sposknikovan injection of the present invention's preparation is weak yellow liquid, and wherein the content of acidic polysaccharose 〉=20% (in galacturonic acid) is 65% of labelled amount; Protein content 〉=1% (in calf serum) is 35% of labelled amount.
Prevent and treat AIDS at present both at home and abroad, with strengthening vital QI to eliminate pathogenic factors, strengthening the body resistance, improving immune defence capability and killing HIV (human immunodeficiency virus) is main target.Sposknikovan and preparation thereof that the present invention produces have above-mentioned effect, make and make sposknikovan and preparation thereof and will bring into play it and increase the T cell quantity, promote macrophage phagocytic function, promote lymphocyte transformation, will bring into play inhibitory action for the AIDS patient owing to malignant tumor takes place immunologic inadequacy, kill HIV (human immunodeficiency virus) simultaneously
Sposknikovan and preparation thereof improve, and experimental result extracts immune, that kill the HIV (human immunodeficiency virus) effect are as follows: the polysaccharide of obtaining through refining from Chinese medicine Radix Saposhnikoviae MT4 method, external anti-HIV-1 effect suppression ratio is 40-50% (1:100).
Claims (12)
1, a kind of preparation method of Chinese medicine sposknikovan is characterized in that the Radix Saposhnikoviae medical material after crushed, with the lower alcohol defat of high concentration, the defat medical material decocts boiling to carry in the pot, the elimination leach cooking liquid, and medicinal residues are with aqueous alkali room temperature dipping three times, merge three immersion or with Radix Saposhnikoviae defat medical material directly with alkali liquor room temperature dipping three times, after merging three immersion and neutralizing, be concentrated into rare fluid extract state with acid, put in 3000 rev/mins of centrifuges, centrifugal 10-20 minute, discard precipitate, get supernatant and add lower alcohol again, after fully stirring, more than cold preservation 8-36 hour, siphon goes out supernatant, precipitate dries with centrifuge, drying, dried precipitate is dissolved in water, the filtering insoluble matter, filtrate is dialysed and ultra-filtration, collects circulation fluid, concentrates, adding lower alcohol to concentration is more than 80%, cold preservation, centrifugal, drying precipitate gets sposknikovan.
2, preparation method as claimed in claim 1 is characterized in that used Chinese medicine Radix Saposhnikoviae is the certified products Radix Saposhnikoviae.
3, preparation method as claimed in claim 1 is characterized in that used alkali is hydroxide, carbonate, acetate and the ammonium hydroxide of monovalent metallic ion.
4, preparation method as claimed in claim 1 is characterized in that used acid is 36% acetic acid and glacial acetic acid.
5, preparation method as claimed in claim 1 is characterized in that alkali steeping three times, and solid-to-liquid ratio is 1: 6-12, the time is 0.5-2 hour.
6, preparation method as claimed in claim 1 is characterized in that impregnation liquid and surpasses the filtrate thickening temperature carrying out≤60 ℃ of water-baths.
7, preparation method as claimed in claim 1 is characterized in that dialysis time is 16-72 hour.
8, preparation method as claimed in claim 1, dialyzer of using when it is characterized in that dialysing and bag filter and ultrafilter membrane, its aperture is 60,000 molecular weight.
9, preparation method as claimed in claim 1 is characterized in that the lower alcohol that adds is methanol, ethanol and propanol, the alcohol amount 〉=75% that contains that reaches.
10, a kind of preparation method of sposknikovan injection is characterized in that injection is that the sposknikovan that the described method of claim 1 prepares is mixed with 2-10mg/ml with aqueous solution, transfers pH value to 8 with 1% sodium hydroxide, and heating for dissolving is filtered; Filtrate through heat treatment cold preservation once refilters, and mends and adds water to recipe quantity, fine straining, fill, sterilization.
11, sposknikovan injection preparation method according to claim 11 is characterized in that described alkali is sodium hydroxide.
12, sposknikovan injection preparation method according to claim 11 is characterized in that the injection pH value is 7.5-9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95104920A CN1065751C (en) | 1995-05-10 | 1995-05-10 | Ledebouriella polysaccharide and its preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN95104920A CN1065751C (en) | 1995-05-10 | 1995-05-10 | Ledebouriella polysaccharide and its preparation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1135900A CN1135900A (en) | 1996-11-20 |
| CN1065751C true CN1065751C (en) | 2001-05-16 |
Family
ID=5075312
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN95104920A Expired - Fee Related CN1065751C (en) | 1995-05-10 | 1995-05-10 | Ledebouriella polysaccharide and its preparation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1065751C (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101474237B (en) * | 2007-12-18 | 2012-05-30 | 北京康仁堂药业有限公司 | Detection method of trichosanthes dispensing granule |
| CN110117332B (en) * | 2018-02-05 | 2022-03-04 | 上海绿谷制药有限公司 | Separated radix sileris polysaccharide and application thereof |
-
1995
- 1995-05-10 CN CN95104920A patent/CN1065751C/en not_active Expired - Fee Related
Non-Patent Citations (3)
| Title |
|---|
| 中国药学杂志27(6) 1992.1.1 王建华等，中药防风的研究概况 * |
| 中草药25(8) 1994.1.1 马红，防风近年研究概述 * |
| 中草药25(8) 1994.1.1 马红，防风近年研究概述;中国药学杂志27(6) 1992.1.1 王建华等，中药防风的研究概况 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1135900A (en) | 1996-11-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102030834B (en) | Method for extracting and preparing camellia polysaccharide from camellia and application of camellia polysaccharide | |
| CN109846940A (en) | A kind of Siberian solomonseal rhizome polysaccharide extract and its extracting method and purposes | |
| US9662349B2 (en) | Dietary composition having mixed polysaccharides derived from lucid ganoderma, lycium barbarum and polygonatum sibiricum and method of its preparation | |
| CN103735633B (en) | A kind of preparation method of instant gardenia powder | |
| CN102652829A (en) | Extraction method of ginseng peptide | |
| CN109608557A (en) | Polysaccharides extracts Isolation and purification method, Lycium chinense glycopeptide and preparation method | |
| CN105504079A (en) | Process for producing astragalus polysaccharide by using ultrasonic technology | |
| CN101974095B (en) | Method for extracting and separating Chinese narcissus polysaccharides | |
| CN109601795A (en) | Using penthorum chinense pursh as composition alcohol-decomposing beverage of primary raw material and preparation method thereof | |
| CN112057546A (en) | Propolis ganoderma lucidum spore powder composition and preparation method and application thereof | |
| CN1065751C (en) | Ledebouriella polysaccharide and its preparation | |
| CN105030812A (en) | Compound fungal polysaccharide capsule | |
| CN102659958A (en) | Dandelion polysaccharide extract and preparation method and application thereof | |
| CN104725218A (en) | Method for extracting and purifying hydroxycitric acid from garcinia cambogia | |
| WO2017185289A1 (en) | Method for preparing active extract of sea buckthorn | |
| CN105535286A (en) | Traditional Chinese medicine composition for resisting tumors in internal medicine | |
| CN1098628A (en) | Coptotermes formosanus Shtrari. and bacterium plot series preparations thereof | |
| CN103146476A (en) | Green extraction technique capable of comprehensively and efficiently utilizing sophora flower bud | |
| CN112442136A (en) | Method for extracting functional components from tremella | |
| CN109457009B (en) | Preparation method of micromolecule astragalus membranaceus chelating peptide | |
| CN114470148A (en) | Natural compound biological agent for resisting swine fever virus as well as preparation method and application thereof | |
| JP2004500313A (en) | Powder bath formulation with active ingredients from natural medicinal plants and their pre-treated products | |
| CN101979003A (en) | Antitumor traditional Chinese medicine | |
| CN101961394A (en) | Traditional Chinese medicine for treating tumors | |
| CN112156122A (en) | Micromolecular codonopsis pilosula instant powder and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C57 | Notification of unclear or unknown address | ||
| DD01 | Delivery of document by public notice |
Addressee: Beijing University of Chinese Medicine Document name: payment instructions |
|
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |