CN106562115A - Marine fish microbial preparation composition and application thereof - Google Patents
Marine fish microbial preparation composition and application thereof Download PDFInfo
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- CN106562115A CN106562115A CN201610947255.8A CN201610947255A CN106562115A CN 106562115 A CN106562115 A CN 106562115A CN 201610947255 A CN201610947255 A CN 201610947255A CN 106562115 A CN106562115 A CN 106562115A
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Abstract
The invention discloses a marine fish microbial preparation composition and application thereof. The microbial preparation composition is prepared from the following raw materials in parts by weight: 10-20 parts of pu'er tea probiotic bacterial powder, 10-20 parts of synanthrin, 20-30 parts of fructooligosaccharide, 10-20 parts of ga-lactooligosaccharides, 3-8 parts of glycine betaine, 1-4 parts of polyvinylpyrrolidone, 0.01-0.05 part of copper sulfate, 10-20 parts of beta-glucan and 10-20 parts of white carbon black. The marine fish microbial preparation composition does not have the problem of single application, and can be used for effectively improving the survival rate of marine fish.
Description
Technical field
The present invention relates to a kind of microorganism formulation, more particularly, to a kind of marine fish microorganism formulation compositionss and its use
On the way.
Background technology
Miscellaneous microorganism species are there are in fish digestive tube, in growth and development process, the digestive tract of Fish
In gradually formed a dynamic Bacterial community being made up of aerobic bacteria, facultative anaerobic bacteria and absolute anaerobe.In normal condition
Under, the various growth of microorganism of fish digestive tube are good, and accounting for the beneficial microbe colony of dominant advantage can make digestive tract in strong
Health situation, so as to ensure normal stool and absorption of the Fish to food.Additionally, beneficial microbe colony is colonized in enteral wall gluing
Film, forms nonspecific biological protection barrier, can suppress field planting of the pathogen to intestinal wall.
However, go from bad to worse with breeding environment, abuse of antibiotics, unbalanced feed nutrition the problems such as take place frequently, Fish
Intestinal microflora structure receives different degrees of impact, and body normal defense system is destroyed, some conditioned pathogens
Can shift, be colonized and attack other histoorgans of fish body so that immunity degradation, the susceptibility to disease strengthen, and finally lead
Bacterial disease is caused to take place frequently.Therefore, it is necessary to provide a kind of microorganism formulation, maintenance is played（Or rebuild）Fish digestive tube it is strong
Health microbial bacteria group structure, the effect of enhance immunity.
Such as prior art Authorization Notice No.：The Chinese invention patent of CN101326958B, a kind of regulation of the disclosure of the invention
The peptide feedstuff additive of immunity function of sea water fish.It is the molecule produced through enzyme hydrolysiss by the morrhua flesh of fish and fish skin, internal organs
Amount scope is respectively the peptide of 100～500Da, 500～1500Da, 1500～3000Da, 3000～5000Da, 5000～8000Da
In three kinds or more constitute in proportion.The immunomodulator of the invention of addition 1～5%, can substantially carry in feedstuff
The level of IgM and lysozyme in high sea water fish serum；The immunomodulator of the invention can also improve cultured fishes liver SOD water
It is flat, so as to improve its oxidation resistance.The immunomodulating peptides additive of the invention can also significantly improve Fish daily gain, drop
Low liver body index.The peptide feedstuff additive of the regulation immunity function of sea water fish of the invention is safe, nuisanceless, using this additive
The fish of cultivation meets pollution-free food hygienic requirements, but the feed additive and inapplicable and most of sea water fingerling, exists applicable
It is single, and it improves really in survival rate, but effect is not obvious.
The content of the invention
The technical problem to be solved is to provide one kind and does not exist applicable single, effectively improves marine fish survival rate
Marine fish microorganism formulation compositionss and application thereof.
The present invention solve the technical scheme that adopted of above-mentioned technical problem for：Marine fish microorganism formulation compositionss and its use
On the way, wherein：
Folium camelliae assamicae probiotic bacteria is formed in ripe tea sweat, raw tea is positioned in the drying baker that constant temperature can divulge information and is fermented, so
Strain is separated afterwards and is added in culture medium, shaken up, be placed in 1 ~ 2d of culture in incubator, be dried gained；
Microorganism formulation compositionss are made up of following raw material and its parts by weight：10～20 parts of Folium camelliae assamicae probiotic bacteria mycopowder, inulin
10～20 parts, 20～30 parts of fruit oligose, 10～20 parts of galactooligosacchari(es, 3～8 parts of glycine betaine, 1～4 part of polyvinylpyrrolidone, sulfur
0.01～0.05 part of sour copper, 10～20 parts of beta glucan, 10～20 parts of white carbon, are suitable for and there is no unicity, to most of sea
Water fish class has excellent effect, because its mechanism of action it is indefinite, or because synergism causes its effectively suppression to cure the disease microorganism
Breeding and stimulate the growth of profitable strain, be provided simultaneously with excellent antibacterial ability, because of the addition of glycine betaine, make marine fish more frequency
Numerous ingesting increases its heavy rate, and the drug effect prolongation effect that cooperates, and so as to improve immunity of sea water fish, improves its survival rate.
Preferably, microbial composite is made up of following raw material and its parts by weight：15 parts of Folium camelliae assamicae probiotic bacteria mycopowder,
15 parts of inulin, 25 parts of fruit oligose, galactooligosacchari(es 15,5 parts of glycine betaine, 1 part of polyvinylpyrrolidone, 0.015 part of copper sulfate, β-Portugal
15 parts of polysaccharide, 15 parts of white carbon.
Or, microbial composite is made up of following raw material and its parts by weight：20 parts of Folium camelliae assamicae probiotic bacteria mycopowder, chrysanthemum
10 parts of sugar, 20 parts of fruit oligose, 8 parts of 10 glycine betaine of galactooligosacchari(es, 4 parts of polyvinylpyrrolidone, 0.05 part of copper sulfate, beta glucan
10 parts, 20 parts of white carbon.
Or, microbial composite is made up of following raw material and its parts by weight：10 parts of Folium camelliae assamicae probiotic bacteria mycopowder, chrysanthemum
20 parts of sugar, 30 parts of fruit oligose, galactooligosacchari(es 20,3 parts of glycine betaine, 3 parts of polyvinylpyrrolidone, 0.03 part of copper sulfate, β-Portugal gather
Sugared 20 parts, 10 parts of white carbon.
Microorganism formulation compositionss are used to prepare the purposes of the microorganism formulation compositionss for improving immunity of sea water fish.
Addition of the microorganism formulation compositionss in marine fish feed is 5～10%, is suitable for major part seawater fish.
Microorganism formulation compositionss outside 5～6% dosage by being sprayed in marine fish feedstuff.
Microorganism formulation compositionss are mixed in marine fish feedstuff by 8～10% dose uniformity.
Compared with prior art, it is an advantage of the current invention that：There is no unicity in the present invention, to most of seawater fish all
There is excellent effect, the breeding of the microorganism that effectively suppresses to cure the disease simultaneously stimulates the growth of profitable strain, is provided simultaneously with excellent antibacterial
Ability, because of the addition of glycine betaine, makes frequent the ingesting of marine fish increase its heavy rate, and the drug effect prolongation effect that cooperates, so as to carry
High immunity of sea water fish, improves its survival rate.
Specific embodiment
The present invention is described in further detail with reference to embodiments.
Embodiment 1：
Folium camelliae assamicae probiotic bacteria is formed in ripe tea sweat, raw tea is positioned in the drying baker that constant temperature can divulge information and is fermented, so
Strain is separated afterwards and is added in culture medium, shaken up, be placed in 1 ~ 2d of culture in incubator, be dried gained；
Microorganism formulation compositionss are made up of following raw material and its parts by weight：10～20 parts of Folium camelliae assamicae probiotic bacteria mycopowder, inulin
10～20 parts, 20～30 parts of fruit oligose, 10～20 parts of galactooligosacchari(es, 3～8 parts of glycine betaine, 1～4 part of polyvinylpyrrolidone, sulfur
0.01～0.05 part of sour copper, 10～20 parts of beta glucan, 10～20 parts of white carbon, are suitable for and there is no unicity, to most of sea
Water fish class has excellent effect, because its mechanism of action it is indefinite, or because synergism causes its effectively suppression to cure the disease microorganism
Breeding and stimulate the growth of profitable strain, be provided simultaneously with excellent antibacterial ability, because of the addition of glycine betaine, make marine fish more frequency
Numerous ingesting increases its heavy rate, and the drug effect prolongation effect that cooperates, and so as to improve immunity of sea water fish, improves its survival rate, below
The present inventor by carrying out contrast experiment with routine techniquess, if matched group（15 parts of Folium camelliae assamicae probiotic bacteria mycopowder, 15 parts of inulin, fruit
25 parts of oligosaccharide, galactooligosacchari(es 15,15 parts of beta glucan, 15 parts of white carbon）；Embodiment group（15 parts of Folium camelliae assamicae probiotic bacteria mycopowder,
15 parts of inulin, 25 parts of fruit oligose, galactooligosacchari(es 15,5 parts of glycine betaine, 1 part of polyvinylpyrrolidone, 0.015 part of copper sulfate, β-Portugal
15 parts of polysaccharide, 15 parts of white carbon）, in the case where other conditions are consistent, experimental result such as following table：
| Rate of body weight gain（%） | Survival rate（%） | |
| Matched group | 7.12 | 67.73 |
| Embodiment group | 10.11 | 80.45 |
As can be seen from the table, embodiment group is all higher than matched group in rate of body weight gain and survival rate, because its data claims non-linear, effect machine
Reason is still to be tested.
Preferably, microbial composite is made up of following raw material and its parts by weight：15 parts of Folium camelliae assamicae probiotic bacteria mycopowder,
15 parts of inulin, 25 parts of fruit oligose, galactooligosacchari(es 15,5 parts of glycine betaine, 1 part of polyvinylpyrrolidone, 0.015 part of copper sulfate, β-Portugal
15 parts of polysaccharide, 15 parts of white carbon.
Or, microbial composite is made up of following raw material and its parts by weight：20 parts of Folium camelliae assamicae probiotic bacteria mycopowder, chrysanthemum
10 parts of sugar, 20 parts of fruit oligose, 8 parts of 10 glycine betaine of galactooligosacchari(es, 4 parts of polyvinylpyrrolidone, 0.05 part of copper sulfate, beta glucan
10 parts, 20 parts of white carbon.
Or, microbial composite is made up of following raw material and its parts by weight：10 parts of Folium camelliae assamicae probiotic bacteria mycopowder, chrysanthemum
20 parts of sugar, 30 parts of fruit oligose, galactooligosacchari(es 20,3 parts of glycine betaine, 3 parts of polyvinylpyrrolidone, 0.03 part of copper sulfate, β-Portugal gather
Sugared 20 parts, 10 parts of white carbon.
Microorganism formulation compositionss are used to prepare the purposes of the microorganism formulation compositionss for improving immunity of sea water fish.
Addition of the microorganism formulation compositionss in marine fish feed is 5～10%, is suitable for major part seawater fish.
Microorganism formulation compositionss outside 5～6% dosage by being sprayed in marine fish feedstuff.
Microorganism formulation compositionss are mixed in marine fish feedstuff by 8～10% dose uniformity.
Embodiment 2：
The preparation of Folium camelliae assamicae probiotic bacteria mycopowder：Raw tea is positioned in the drying baker that constant temperature can divulge information, every 4 h is carried out once
Ventilation, thermometric, weigh and turning process, ferment 30h, be then peeled off strain and add in culture medium, shake up, be placed in
Culture 48h in 28. 5 DEG C of constant incubators, is dried gained.
Weigh 150 grams of Folium camelliae assamicae probiotic bacteria mycopowder, 250 grams of fruit oligose, 150 grams of inulin, 50 grams of glycine betaine, polyvinyl pyrrole
10 grams of alkanone, 0.15 gram of copper sulfate, 150 grams of beta glucan, 150 grams of galactooligosacchari(es, 150 grams of white carbon, are mixed to prepare microorganism
Preparation, by being sprayed on outside 5% dosage in marine fish feedstuff, carries out feeding and throws.
Embodiment 2：
Weigh 100 grams of Folium camelliae assamicae probiotic powder, 300 grams of fruit oligose, 100 grams of inulin, 30 grams of glycine betaine, polyvinylpyrrolidone 4
Gram, 0.3 gram of copper sulfate, 200 grams of beta glucan, 100 grams of galactooligosacchari(es, 200 grams of white carbon are mixed to prepare microorganism formulation, press
9% dose uniformity is mixed in marine fish feedstuff, is carried out feeding and is thrown.
Contrast experiment 1：
A kind of microorganism formulation compositionss, its effective ingredient include Folium camelliae assamicae probiotic bacteria mycopowder, fruit oligose, inulin, beta glucan,
Galactooligosacchari(es, white carbon.Weigh Folium camelliae assamicae probiotic bacteria mycopowder 150g, fruit oligose 250g, inulin 150g, glycine betaine 50g, polyethylene
Ketopyrrolidine 10g, copper sulfate 0.15g, beta glucan 150g, galactooligosacchari(es 150g, white carbon 150g, are mixed to prepare microorganism system
Agent, by 4%, 8%, 12% dosage tiger speckle special feed is respectively added to（Nanhai District Foshan City salt walks global Aquatic product trade market to be had
Limit company, tiger speckle special feed）In be configured to test feedstuff, and will not contain this compositions tiger speckle feedstuff as control raise
Material.Feed original body mass（22.0±2.0）The tiger speckle of g counts Gain weight after 16 weeks, and Aeromonas hydrophila is carried out afterwards
Challenge test, counts survival rate, the results are shown in Table lattice 1：
| Rate of body weight gain (%) | Survival rate (%) | |
| 4% addition group | 7.16 | 77.70% |
| 8% addition group | 10.27 | 81.93% |
| 12% addition group | 10.35 | 81.29% |
| Matched group | 6.99 | 69.21% |
From form 1, experimental group rate of body weight gain is 7.16～10.35%, and matched group rate of body weight gain is 6.99%；Infect thermophilic aqueous vapor unit cell
After bacterium, experimental group survival rate is 77.70%～81.93%, and matched group survival rate is 69.21%.
Contrast experiment 2：
Weigh 150 grams of Folium camelliae assamicae probiotic bacteria mycopowder, 250 grams of fruit oligose, 150 grams of inulin, 50 grams of glycine betaine, polyvinylpyrrolidone
10 grams, 0.15 gram of copper sulfate, 150 grams of beta glucan, 150 grams of galactooligosacchari(es, 150 grams of white carbon, be mixed to prepare microorganism formulation.
Jinchang fish meal is sprayed on respectively by 4%, 6%, 8% dosage using trypsin method technology（Tianjin Fisheries Research Laboratory, special feed）Surface
As experiment feedstuff, and using the Jinchang fish meal without this compositions as control feed.Feed original body mass（45.0±1.0）
The Jinchang fish of g counts Gain weight after 16 weeks, and the challenge test of Aeromonas hydrophila is carried out afterwards, counts survival rate, as a result sees
Form 2：
| Rate of body weight gain（%） | Survival rate（%） | |
| 4% additive | 18.07 | 76.49% |
| 6% additive | 26.56 | 85.33 |
| 8% additive | 39.78 | 89.67% |
| Matched group | 15.59 | 69.56% |
As seen from the above table, experimental group rate of body weight gain is 18.07～39.78%, and matched group rate of body weight gain is 15.59%；Infect thermophilic aqueous vapor list
After born of the same parents bacterium, experimental group survival rate is 76.49%～89.67%, and matched group survival rate is 69.56%.
Contrast test 3：
Weigh 150 grams of Folium camelliae assamicae probiotic bacteria mycopowder, 250 grams of fruit oligose, 150 grams of inulin, 50 grams of glycine betaine, polyvinylpyrrolidone
10 grams, 0.15 gram of copper sulfate, 150 grams of beta glucan, 150 grams of galactooligosacchari(es, 150 grams of white carbon, be mixed to prepare microorganism formulation.
Jinchang fish meal is sprayed on respectively by 4%, 6%, 8% dosage using trypsin method technology（Tianjin Fisheries Research Laboratory, special feed）Surface
As experiment feedstuff, Jinchang fish meal is mixed in as a control group with existing additive, feeds original body mass（45.0±1.0）g
Jinchang fish after 10 weeks, stop using the Jinchang fish meal containing additive to be changed to common Jinchang fish meal and feed 10 weeks, as a result
Such as table 3 below：
| Rate of body weight gain（%） | Survival rate（%） | Prevalence（%） | |
| Embodiment group | 30.61 | 83.78 | 21.34 |
| Matched group | 16.33 | 61.51 | 34.98 |
Learnt by upper table, the present embodiment is all higher than matched group in rate of body weight gain, survival rate, prevalence is less than matched group, can see
Go out the present embodiment product can effectively maintain marine fish digestive system health, so as to improve immunity of sea water fish.
Jing inventor test result indicate that, mentioned microorganism preparation is added to the feeding of seawater fish by 5～10% dosage
In feed composition, test the survival rate after fish rate of body weight gain, survival rate and infection Aeromonas hydrophila and significantly improve.Present invention operation
It is upper less demanding, it is easily mastered and implements, cultured fishes and people will not be damaged.
Certainly, described above not limitation of the present invention, the present invention is also not limited to the example above.The art
In the essential scope of the present invention, change, remodeling, addition or the replacement made should also belong to the present invention's to those of ordinary skill
Protection domain.
Claims (6)
1. marine fish microorganism formulation compositionss and application thereof, it is characterised in that：The microorganism formulation compositionss are by following original
Material and its parts by weight composition：10～20 parts of Folium camelliae assamicae probiotic bacteria mycopowder, 10～20 parts of inulin, 20～30 parts of fruit oligose, gala
10～20 parts of oligosaccharide, 3～8 parts of glycine betaine, 1～4 part of polyvinylpyrrolidone, 0.01～0.05 part of copper sulfate, beta glucan 10
～20 parts, 10～20 parts of white carbon.
2. marine fish microorganism formulation compositionss according to claim 1 and application thereof, it is preferred that it is characterized in that：Institute
State microbial composite to be made up of following raw material and its parts by weight：15 parts of Folium camelliae assamicae probiotic bacteria mycopowder, 15 parts of inulin, fruit it is few
25 parts of sugar, galactooligosacchari(es 15,5 parts of glycine betaine, 1 part of polyvinylpyrrolidone, 0.015 part of copper sulfate, 15 parts of beta glucan, Linesless charcoal
Black 15 parts.
3. marine fish microorganism formulation compositionss and application thereof, it is characterised in that：The microorganism formulation compositionss are used to prepare
Improve the purposes of the microorganism formulation compositionss of immunity of sea water fish.
4. marine fish microorganism formulation compositionss according to claim 3 and application thereof, it is characterised in that：The microorganism
Addition of the preparation compositions in marine fish feed is 5～10%.
5. marine fish microorganism formulation compositionss according to claim 4 and application thereof, it is characterised in that：The microorganism
Preparation compositions outside 5～6% dosage by being sprayed in marine fish feedstuff.
6. marine fish microorganism formulation compositionss according to claim 4 and application thereof, it is characterised in that：The microorganism
Preparation compositions are mixed in marine fish feedstuff by 8～10% dose uniformity.
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| CN101326958A (en) * | 2008-07-22 | 2008-12-24 | 浙江大学 | Peptide feedstuff additive for regulating immunity function of sea water fish |
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| CN101326958A (en) * | 2008-07-22 | 2008-12-24 | 浙江大学 | Peptide feedstuff additive for regulating immunity function of sea water fish |
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| CN105724830A (en) * | 2016-02-25 | 2016-07-06 | 南京农业大学 | Composite feed attractant for fishing and preparation method and application thereof |
| CN105746955A (en) * | 2016-03-22 | 2016-07-13 | 五河县金塘水产养殖有限公司金贸园分公司 | Fully-cooked granulated feed for greenhouse breeding of soft-shelled turtles and preparation method of feed |
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Application publication date: 20170419 |