CN106540958A - A kind of method of utilization phosphate solubilizing bacteria fortification of plants cadmium pollution soil repair - Google Patents
A kind of method of utilization phosphate solubilizing bacteria fortification of plants cadmium pollution soil repair Download PDFInfo
- Publication number
- CN106540958A CN106540958A CN201710023662.4A CN201710023662A CN106540958A CN 106540958 A CN106540958 A CN 106540958A CN 201710023662 A CN201710023662 A CN 201710023662A CN 106540958 A CN106540958 A CN 106540958A
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- soil
- cadmium
- cadmium pollution
- plant
- phosphate solubilizing
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/08—Reclamation of contaminated soil chemically
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
Abstract
The invention discloses a kind of method of utilization phosphate solubilizing bacteria fortification of plants cadmium pollution soil repair, comprises the following steps：（1）Phosphorus decomposing bacteria agent is uniformly applied in cadmium pollution soil according to 10 ~ 50 kgs/acre of applied amount, is turned over；Apply base manure again and turn over again；（2）Super enriching plant is planted in cadmium pollution soil, is treated that super enriching plant is harvested first 10 ~ 15 days, is applied the chelating agen of 100 ~ 300 kg/ mus in soil.The present invention combines chelating agen fortification of plants recovery technique using phosphate solubilizing bacteria, gives full play to both synergism, compared to other physical chemistry recovery techniques, has the advantages that cheap, non-secondary pollution, simple to operate and repairing effect are good.
Description
Technical field
The present invention is a kind of method of utilization phosphate solubilizing bacteria fortification of plants cadmium pollution soil repair, belongs to plant soil restoration weight
Metallic pollution technical field.
Background technology
Heavy metal refers to that density is more than 5g/cm3Metal, or in the periodic table of elements metal grid atomic weight more than more than 40
Metallic element be referred to as heavy metal element.In recent years, common environmental pollution heavy metal has lead, cadmium, chromium, hydrargyrum and metalloid arsenic
Etc. the notable metallic element of bio-toxicity.Wherein, used as one of modal element of heavy metal pollution of soil, its toxicity is larger for cadmium,
Serious to harm and slower in human body metabolism by the air and food of cadmium pollution, once there is " pain pain because of cadmium poisoning in Japan
Disease ", Ministry of Agriculture's rice in 2002 and quality of item supervision and inspection center carry out safety sampling observation knot to national market rice
Fruit display, cadmium exceeding standard rate 10.3% in rice.Just because of the appearance of China's cadmium rice pollution, thing of the people to Cadmium Pollution in Soils
The attention that part is also slowly produced, therefore, the improvement to Cadmium Pollution in Soils has become current emphasis and hot issue.
At present, heavy metal-polluted soil recovery technique mainly has physical restoration, chemical restoration, biological restoration etc., plant
Recovery technique is a kind of cheap, green, easy-operating heavy metal based technique for in-situ remediation relative to other recovery techniques, is to transport at present
With the method for relatively broad improvement heavy metal-polluted soil.The essence of phytoremediation technology is exactly to plant in heavy-metal contaminated soil
Heavy metal super-enriched plant, using the super enriching plant accumulation ability powerful to heavy metal-polluted soil, the heavy metal in soil is inhaled
Receipts are entered in plant, are focused on this kind of super enriching plant after plant maturation after harvesting, so as to reach removal
The purpose of heavy metal-polluted soil.Although super enriching plant heavy metal has stronger enrichment, most super enriching plant lifes
Long period length, plant are short and small and Biomass is low, and another is exactly that the biological effectiveness of heavy metal in soil is relatively low, is difficult by plant
Extract and absorb.Therefore, the biological effectiveness and raising phytomass for how increasing heavy metal-polluted soil becomes raising plant and repaiies
The key of multiple effect.
The content of the invention
The technical problem to be solved in the present invention is to overcome the deficiencies in the prior art, is directed to Cd-polluted farmland soil, there is provided
A kind of utilization phosphate solubilizing bacteria combines the method for chelating agen fortification of plants cadmium pollution soil repair, by planting in cadmium pollution soil
With ore deposit Herba hylotelephii erythrosticti, and phosphate solubilizing bacteria is inoculated with soil and applies chelating agen, so as to improve the Biomass and heavy metal-polluted soil of plant
Biological effectiveness, the effect of fortification of plants repairing heavy metal in soil cadmium is that a kind of environmentally friendly, honest, efficient heavy metal-polluted soil is repaiied
Recovering technology.
The technical scheme is that, there is provided a kind of method of utilization phosphate solubilizing bacteria fortification of plants cadmium pollution soil repair, wrap
Include following steps：
(1) phosphorus decomposing bacteria agent is uniformly applied in cadmium pollution soil according to 10～50 kgs/acre of applied amount, is turned over
Plough；Apply base manure again and turn over again；
(2) super enriching plant is planted in cadmium pollution soil, treat that super enriching plant is harvested first 10～15 days, apply in soil
Plus the chelating agen of (root particularly in super enriching plant applies) 100～300kg/ mus.
Further, the phosphate solubilizing bacteria is bacillus megaterium.
Further, the super enriching plant is with ore deposit Herba hylotelephii erythrosticti.
Further, the ten thousand plants/hm of planting density 40-50 with ore deposit Herba hylotelephii erythrosticti2。
Further, described chelating agen is EDDS.
Further, in step (2), by the seedling replanting of super enriching plant in cadmium pollution soil.
Further, the cadmium content of the cadmium pollution soil is 0.3～1.0mg/kg, and pH is 4.5～6.5.
Further, the main component of described base manure is KH2PO4And NH4(SO4)2, applied amount be 50～100 kilograms/
Mu.
With ore deposit Herba hylotelephii erythrosticti as the recent a kind of new zinc Cd-hyperaccumulator for finding, compared to other super enriching plants,
Its Biomass is big, repairing effect has in the reparation of cadmium pollution soil the good market should be potential fortunately.In order to further
The effect of fortification of plants repairing heavy metal in soil, a large amount of scholar's research discoveries, adds chelating agen in heavy-metal contaminated soil
(EDTA, EDDS, DTPA etc.) can utilize the heavy metal ion of free state in chelating agen and soil to carry out complexation, so as to break
Ionic equilibrium in script soil between liquid phase and solid phase so that heavy metal is parsed from soil particle surface, increases soil
Heavy metal ions concentration in solution, improves the biological effectiveness of heavy metal, strengthens the effect of plant extract.Also there is research table
It is bright, microorganism is introduced in soil, on the one hand can be improved plant rhizosphere environment using microorganism, be promoted suction of the plant to nutrient
Receive, improve the Biomass of plant；On the other hand the metabolic activity of itself and soil weight can be passed through in soil using microorganism
There is a series of effects such as absorption, precipitation, oxidoreduction in metal, change form of the heavy metal in soil, promote plant pair
The absorption of heavy metal-polluted soil.The present invention repairs cadmium soil agricultural land soil using phosphate solubilizing bacteria joint chelating agen fortification of plants, fully sends out
Both synergism are waved, the effect of phytoremediation cadmium pollution is greatly improved.
When agricultural land soil cadmium content is 0.3～0.6mg/kg, the applied amount of phosphorus decomposing bacteria agent is 10～30kg/ mus, chela
The dosage of mixture is 100-150kg/ mus；When agricultural land soil cadmium content is 0.6～1.0mg/kg, the inoculation of phosphorus decomposing bacteria agent
Measure as 30～50kg/ mus, the dosage of chelating agen is 150-300kg/ mus.
Compared with prior art, beneficial effects of the present invention are as follows：
(1) it is of the invention using Cd-hyperaccumulator-companion's ore deposit Herba hylotelephii erythrosticti as test plant, compared to other Cd-hyperaccumulators,
Its biomass is high, and fast growth, repairing effect are good.
(2) present invention introduces phosphate solubilizing bacteria in cadmium pollution soil, can improve plant rhizosphere environment and heavy metal in soil
Middle form, promotes the growth of plant, improves the repairing effect of plant heavy metal cadmium.
(3) present invention selects new biodegradable chelating agen EDDS (EDDS), by dirty to cadmium
Add chelating agen in dye soil, improve the biological effectiveness of heavy metal, significantly enhance the effect of plant extract heavy metal cadmium.
(4) present invention combines chelating agen fortification of plants recovery technique using phosphate solubilizing bacteria, gives full play to both synergism,
Compared to other physical chemistry recovery techniques, have the advantages that cheap, non-secondary pollution, simple to operate and repairing effect are good.
Description of the drawings
Impact under the process of Fig. 1 different tests to aboveground vegetation part Biomass.
Impact under the process of Fig. 2 different tests to aboveground vegetation part cadmium content.
Impact under the process of Fig. 3 different tests to Cadmium in Soil content.
Specific embodiment
Below in conjunction with specific embodiment, the invention will be further described, but protection not thereby limiting the invention
Scope.
This test site is lain fallow phase farmland positioned at Changsha, Hunan county cadmium pollution Oryza sativa L., have chosen two pieces of cadmiums respectively
Used as experimental plot, wherein A is the acid soil of cadmium mild or moderate pollution to the different rice terrace of pollution level, and B is serious pollution in cadmium
Acid soil.The concrete physicochemical property in the experimental plot is shown in Table 1.
1 experimental plot cadmium pollution paddy soil basic physical and chemical of table
| Experimental plot | pH | Organic matter (g/kg) | Available state Cd (mg/kg) | Full dose Cd (mg/kg) |
| A | 5.61 | 32.87 | 0.199 | 0.427 |
| B | 5.37 | 24.75 | 0.363 | 0.694 |
Embodiment experimental design：
This test have chosen two pieces of Cd-polluted farmlands of A, B as experimental plot, respectively 2 experimental plots arrange control fields and
Process field, control field (CK) according to local management by custom school, process field devise altogether 4 process (number respectively be 1.,
2., 3., 4.), each processes 3 repetitions of design.Specific embodiment and field management are as follows：
Blank (CK)：Do not implement reclamation activitiess, be managed according to local peasant household's daily habits；
1. plantation is with ore deposit Herba hylotelephii erythrosticti：Apply 100 pounds/acre of base manure respectively at A, B experimental plot and turned over, then support managing
After liquid culture, plant after 100 days, is removed and is burnt to A, B experimental plot by the consistent companion's ore deposit Herba hylotelephii erythrosticti seedling replanting of root system growing way
Burning process；
2. phosphate solubilizing bacteria+plantation is inoculated with ore deposit Herba hylotelephii erythrosticti：The phosphorus decomposing bacteria agent for applying 20kg/ mus and 40kg/ mus respectively is tried in A, B
Field is tested, turning over makes which uniform with mixing with soil, after 5 days, apply 100 kgs/acre of base manure respectively at A, B experimental plot and turned over
Plough, then will manage nutrient solution culture after the consistent companion's ore deposit Herba hylotelephii erythrosticti seedling replanting of root system growing way to A, B experimental plot, after 100 days, by plant
Remove and carry out burning disposal；
3. plantation is with ore deposit Herba hylotelephii erythrosticti+add chelating agen：Apply 100 kgs/acre of base manure respectively at A, B experimental plot and turned over
Plough, then the consistent companion's ore deposit Herba hylotelephii erythrosticti seedling replanting of root system growing way after nutrient solution culture will be managed and, to A, B experimental plot, treat plant growing 90 days
Afterwards, apply 150kg/ mus and 300kg/ mus chelating agen respectively in A, B experimental plot in plant rhizosphere, after 100 days, plant is removed simultaneously
Carry out burning disposal；
4. it is inoculated with phosphate solubilizing bacteria+plantation companion's ore deposit Herba hylotelephii erythrosticti+add chelating agen：Apply the phosphate solubilizing bacteria of 20kg/ mus and 40kg/ mus respectively
In A, B experimental plot, turn over makes which uniform with mixing with soil to microbial inoculum, after 5 days, applies 100 kgs/acre respectively at A, B experimental plot
Base manure is simultaneously turned over, then will manage nutrient solution culture after the consistent companion's ore deposit Herba hylotelephii erythrosticti seedling replanting of root system growing way to A, B experimental plot, treat
After plant growing 90 days, apply the chelating agen of 150kg/ mus and 300kg/ mus respectively in A, B experimental plot in plant rhizosphere, 100 days
Afterwards, plant is removed and carries out burning disposal.
After plant harvesting, soil and plant sample are gathered, Jing after natural air drying, milled 100 mesh plastic screen is packed soil
It is to be measured；Plant sample is cleaned up with tap water, then is cleaned with distilled water and deionized water.Plant sample is dried, 105 DEG C are placed on
Under complete 30min, dry to constant weight at a temperature of 70 DEG C, claim its weight, pulverization process is being carried out to plant, is being packed to be measured.
Result of the test is shown in Table shown in 2 and Fig. 1～3.
Impact (unit under the process of 2 different tests of table to different shape cadmium content in soil：mg/kg)
From table 2 and Fig. 1～3：(1) Biomass that phosphate solubilizing bacteria significantly improves companion's ore deposit Herba hylotelephii erythrosticti aerial partss is inoculated with, its
Maximal increment is up to 74% and 80%.(2) it is inoculated with phosphate solubilizing bacteria and the cadmium of other forms in soil can be converted into exchange state cadmium, promotees
Enter the absorption of plant so that the cadmium content maximum of soil can reduce by 38% and 39%.(3) add chelating agen to chelate in soil
Cadmium, improve enrichment of the cadmium in plant body in soil, aboveground vegetation part cadmium content maximum improves 20% and 28%.
Summarize described, the present invention is by adopting " inoculation phosphate solubilizing bacteria+plantation companion's ore deposit Herba hylotelephii erythrosticti+add in the cadmium pollution soil of farmland
Plus chelating agen " recovery technique, significantly the Biomass of the plant of raising, promotes adsorption effect of the plant to Cadmium in Soil, and which is repaiied
Multiple effect is up to more than 60%, therefore the present invention has application well in terms of reinforcing is with ore deposit Herba hylotelephii erythrosticti repairing heavy metal in soil cadmium
Value.
Claims (8)
1. a kind of method of utilization phosphate solubilizing bacteria fortification of plants cadmium pollution soil repair, it is characterised in that comprise the following steps：
（1）Phosphorus decomposing bacteria agent is uniformly applied in cadmium pollution soil according to 10 ~ 50 kgs/acre of applied amount, is turned over；Again
Apply base manure and turn over again；
（2）Super enriching plant is planted in cadmium pollution soil, is treated that super enriching plant is harvested first 10 ~ 15 days, is applied 100 in soil
The chelating agen of ~ 300 kg/ mus.
2. the method for claim 1, it is characterised in that the phosphate solubilizing bacteria is bacillus megaterium.
3. the method for claim 1, it is characterised in that the super enriching plant is with ore deposit Herba hylotelephii erythrosticti.
4. the method for claim 1, it is characterised in that the ten thousand plants/hm of planting density 40-50 with ore deposit Herba hylotelephii erythrosticti2。
5. the method for claim 1, it is characterised in that described chelating agen is EDDS.
6. the method for claim 1, it is characterised in that step（2）In, the seedling replanting of super enriching plant is dirty to cadmium
In dye soil.
7. the method for claim 1, it is characterised in that the cadmium content of the cadmium pollution soil is 0.3 ~ 1.0 mg/kg,
PH is 4.5 ~ 6.5.
8. the method for claim 1, it is characterised in that the main component of described base manure is KH2PO4And NH4(SO4)2,
Applied amount is 50 ~ 100 kgs/acre.
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Cited By (8)
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| CN107116096A (en) * | 2017-05-16 | 2017-09-01 | 湖北加德科技股份有限公司 | A kind of pyrolyzing sludge carbon and the united heavy metal soil remediation method of bioanalysis |
| CN107685070A (en) * | 2017-11-02 | 2018-02-13 | 新乡学院 | A kind of heavy metal-polluted soil purification method based on ornamental plant and microorganism |
| CN107999535A (en) * | 2017-12-18 | 2018-05-08 | 新乡学院 | A kind of method using herbaceous plant cadmium pollution soil repair |
| CN108787741A (en) * | 2018-05-24 | 2018-11-13 | 益阳创惠技术服务有限公司 | A kind of preparation and application of plant repairing body for contaminated soil |
| CN109226252A (en) * | 2018-10-25 | 2019-01-18 | 湖南新九方科技有限公司 | A kind of method that biochemical joint reduces lead and cadmium in soil |
| CN110624949A (en) * | 2019-09-30 | 2019-12-31 | 武汉工程大学 | Method for repairing excessive phosphorus pollution of phosphorite waste land by combining indigenous microorganisms and plants |
| CN111229826A (en) * | 2020-03-23 | 2020-06-05 | 贵州万通环保工程有限公司 | Comprehensive remediation method for heavy metal contaminated soil |
| CN113172086A (en) * | 2021-04-22 | 2021-07-27 | 贵州星硕铭越环保科技有限公司 | Drip irrigation restoration method for heavy metal polluted agricultural land |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107116096A (en) * | 2017-05-16 | 2017-09-01 | 湖北加德科技股份有限公司 | A kind of pyrolyzing sludge carbon and the united heavy metal soil remediation method of bioanalysis |
| CN107685070A (en) * | 2017-11-02 | 2018-02-13 | 新乡学院 | A kind of heavy metal-polluted soil purification method based on ornamental plant and microorganism |
| CN107999535A (en) * | 2017-12-18 | 2018-05-08 | 新乡学院 | A kind of method using herbaceous plant cadmium pollution soil repair |
| CN108787741A (en) * | 2018-05-24 | 2018-11-13 | 益阳创惠技术服务有限公司 | A kind of preparation and application of plant repairing body for contaminated soil |
| CN109226252A (en) * | 2018-10-25 | 2019-01-18 | 湖南新九方科技有限公司 | A kind of method that biochemical joint reduces lead and cadmium in soil |
| CN110624949A (en) * | 2019-09-30 | 2019-12-31 | 武汉工程大学 | Method for repairing excessive phosphorus pollution of phosphorite waste land by combining indigenous microorganisms and plants |
| CN110624949B (en) * | 2019-09-30 | 2021-10-12 | 武汉工程大学 | Method for repairing excessive phosphorus pollution of phosphorite waste land by combining indigenous microorganisms and plants |
| CN111229826A (en) * | 2020-03-23 | 2020-06-05 | 贵州万通环保工程有限公司 | Comprehensive remediation method for heavy metal contaminated soil |
| CN113172086A (en) * | 2021-04-22 | 2021-07-27 | 贵州星硕铭越环保科技有限公司 | Drip irrigation restoration method for heavy metal polluted agricultural land |
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