CN106539097A - The water-soluble method with Water insoluble dietary fiber is produced simultaneously using bagasse - Google Patents
The water-soluble method with Water insoluble dietary fiber is produced simultaneously using bagasse Download PDFInfo
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Abstract
The invention discloses a kind of utilization bagasse produces the water-soluble method with Water insoluble dietary fiber simultaneously, belong to biological technical field.The present invention is improved to conventional steam explosion isolation technics, using relatively low boiling temperature and elevated pressures（Inert gas is provided）Condition, and digested using special biology enzyme, it is ensured that high efficiency that bagasse is utilized, high-quality, high yield.The present invention obtains water-soluble and Water insoluble dietary fiber simultaneously using bagasse, and gained dietary fiber purity is high, and process is simple；Simultaneously present invention reduces production cost, improves economic benefit, it is easy to industrialized production.
Description
Technical field
The present invention relates to biological technical field, it is more particularly to a kind of using bagasse produce simultaneously it is water-soluble with it is water-insoluble
The method of dietary fiber.
Background technology
Dietary fiber can be divided into two fundamental types whether to be dissolved in water：Water-soluble dietary fiber（SDF）With non-aqueous
Property dietary fiber（IDF）.Cellulose, lignin are modal Water insoluble dietary fibers, and pectin, natural gum etc. belong to water-soluble
Dietary fiber.The two not only species have difference, and physiologically active also has difference.It was found that water-soluble dietary fiber is more non-aqueous
Soluble dietary fiber has higher physiologically active.Water-soluble dietary fiber can slow down digestion rate and most rapid drainage courage is solid
Alcohol, so the blood sugar that can be allowed in blood and cholesterol are controlled in optimal level, may also help in diabetic reduces pancreas
Island element and triglyceride.Water insoluble dietary fiber can reduce the risk for suffering from intestinal cancer, at the same can by assimilating food in have
Noxious material Constipation and diverticulitis, and lower the toxin of bacterium discharge in alimentary canal.Most plants all contain water solubility
With Water insoluble dietary fiber, so balanced intake water solubility could obtain different benefits from Water insoluble dietary fiber in diet
Place.
Hemicellulose containing mass fraction 15-35% in grass, the overwhelming majority are xylan.Xylan be with
Isosorbide-5-Nitrae-β-D- pyranoid forms wood sugar constitute main chain, with 4- oxygen methyl-glucopyranose aldehydic acid as side chain, be difficult to be disappeared
The heteroglycan of change, is excellent dietary fibre.Xylan has good hydrophilicity, than dietary cellulosic be easier aquation,
Absorption, profit rise.Due to the otherness of side chain, dissolubility of the xylan in water is also variant, wherein there are about mass fraction 40%
For water-soluble dietary fiber, mass fraction 60% for Water insoluble dietary fiber.
Bagasse is sugarcane remaining Main By product Jing after mechanical expression, extraction, accounts for sugarcane squeezing amount mass fraction
24-27%.Bagasse main chemical is cellulose（Mass fraction 46%）, hemicellulose（Mass fraction 26%）, lignin
（Mass fraction 23%）Deng macromolecular substances, in addition with a small amount of（Mass fraction 5%）Ash content, wax etc..Due to cane milling
When operate fully according to food standard, the crude fibre containing up to 95% mass fraction in bagasse, thus bagasse is to prepare meals
The high-quality raw material of fiber.
But have no in prior art using bagasse while producing the water-soluble report with Water insoluble dietary fiber, shadow
The performance of bagasse economic benefit is rung.
The content of the invention
In order to make up the deficiencies in the prior art, the invention provides it is a kind of using bagasse produce simultaneously it is water-soluble with it is non-aqueous
The method of soluble dietary fiber.
The technical scheme is that：
A kind of utilization bagasse produces the water-soluble method with Water insoluble dietary fiber, including step simultaneously：
1）Cooker is inserted after bagasse impurity elimination；
2）The water of bagasse quality 1-20 times is added in cooker, adds alkali to alkali lye mass concentration to be 1-10%；It is passed through steam
To cooker, stop logical steam after being warming up to 60-100 DEG C, inert gas is passed through in cooker, air in cooker is arranged
Only；Then intermittence is passed through steam and inert gas into cooker, maintaining in hydrolytic decomposition pot temperature as 60-100 DEG C and
Pressure is 0.1-1MPa, heat-insulation pressure keeping 10-120 minutes；
3）By the substance release in cooker out, and in being transferred to grinding mill grind；
4）Step 3）Gained slurry is placed in enzymatic vessel, and the water of slurry quality 1-10 times is added in enzymatic vessel, adds compound
Protease, interval 1-3 minute, is passed through inert gas, digests 10-80 minutes；Normal temperature type AMS is subsequently added, 10- is digested
80 minutes；Then pass to steam and be warming up to 60-70 DEG C, be incubated the activity of 2-20 minute inactivators；The compound protease is by having
The alkali protease of standby endopeptidase activity and the Proteinase K for possessing peptide ending enzyme activity are constituted；The normal temperature type AMS is by micro-
Ripple induction gained variation bacillus licheniformis secretion gained AMS, the preference temperature of the normal temperature type AMS is 22-
35℃；
5）Go out after enzyme, separation of solid and liquid obtains filter residue one and filtrate one by the substance release in enzymatic vessel out；
6）The gained sig water of filter residue one is washed several times, separation of solid and liquid, and centralized collection washing lotion is to filtrate one, obtains two He of filter residue
Filtrate two；
7）Diluted acid being added in filtrate two, pH being adjusted to 4-6, after stirring, stable 1-5 hours, separate out precipitation, and separation of solid and liquid is obtained
Filter residue three and filtrate three；
8）The liquid that adds dilute alkali in filter residue three dissolves, then acid out again；Repeat 2-3 time, separation of solid and liquid obtains filter residue four and filtrate four；
9）Four drying of filter residue, crushing, obtain water-soluble dietary fiber；
10）The ethanol of 1-10 times of volume is added in filtrate three, after stirring, stable 1-3 hours are separated out and precipitated, separation of solid and liquid,
Obtain filter residue five and filtrate five；
11）Filter residue five and two drying of filter residue, crushing, obtain Water insoluble dietary fiber.
Preferably, step 4）In, the obtaining step of the microwave induced gained variation bacillus licheniformis is concrete
For：The nutrient solution of bacillus licheniformis is placed in into microwave generator, setting microwave power is 850-950W, pulse frequency is
2300MHz, microwave treatment 20s cool down 20s, reciprocal 25-35 time according to this；Nutrient solution after microwave treatment is coated on into solid training
On foster base, cultivate 1-2 days under the conditions of 30 DEG C, by the high ground of alpha-amylase activity under four plants of normal temperature of screening in the bacterium colony for surviving
The dissociant of clothing bacillus.
Further, the dissociant Amplification Culture of alpha-amylase activity highest bacillus licheniformis under normal temperature is selected,
So as to obtain the normal temperature type AMS.The normal temperature type AMS obtained using the method, at normal temperatures can efficient enzyme
Solution starch, had both reduced energy consumption, turn avoid the generation of side reaction.
Preferably, possess the alkali protease of endopeptidase activity in the compound protease and possess peptide ending enzyme work
Property Proteinase K ratio be 1:1-3；The addition of the compound protease meets every kilogram of butt bagasse 400-800U,
The addition of the normal temperature type AMS meets every kilogram of butt bagasse 300-700U.
Preferably, the alkali is NaOH, potassium hydroxide, ammoniacal liquor or calcium hydroxide；The acid is hydrochloric acid, nitre
Acid or acetic acid.
Preferably, the inert gas is nitrogen, helium or argon gas.
Preferably, step 9）, step 11）Described in be dried for spray drying, heated-air drying, fluidized bed drying or
Freeze-drying；Baking temperature is less than 120 DEG C.
Preferably, step 10）In the volume fraction of ethanol used be 95%.
Beneficial effects of the present invention are：
The present invention is improved to conventional steam explosion isolation technics, using relatively low boiling temperature and elevated pressures（Inert gas
There is provided）Condition, and digested using special biology enzyme, it is ensured that high efficiency that bagasse is utilized, high-quality, high yield.The present invention
Water-soluble and Water insoluble dietary fiber is obtained simultaneously using bagasse, and gained dietary fiber purity is high, and process is simple.
Present invention reduces production cost, improves economic benefit, it is easy to industrialized production.
Specific embodiment
Embodiment 1
A kind of utilization bagasse produces the water-soluble method with Water insoluble dietary fiber, including step simultaneously：
1）Bagasse impurity elimination（Reject big material）After insert cooker；
2）The water of 10 times of bagasse quality is added in cooker, adds potassium hydroxide to alkali lye mass concentration to be 7%；It is passed through steaming
Vapour stops logical steam to cooker, after being warming up to 90 DEG C, is passed through inert nitrogen gas, by air in cooker in cooker
Emptying, sealing；Then intermittence is passed through steam and inert gas into hydrolytic decomposition pot, with maintain in hydrolytic decomposition pot temperature as 95 DEG C with
And pressure be 0.8MPa, heat-insulation pressure keeping 50 minutes；
In the step, adopt relatively low boiling temperature to avoid decomposition reaction, the dehydration of each component（Coking）Reaction, peeling reaction
Deng using elevated pressures（By being passed through inert gas realization）Ensure that alkali lye, to the effective infiltration between bagasse cell membrane, is adopted
Use inert environments（It is passed through inert gas realization）Avoid oxidation reaction, the esterification of acetyl group of each component molecular radical
Deng so as to ensure that integrality, high yield pulp1 and the high-quality of each material；This step diluted alkaline can extract hemicellulose simultaneously
Extract the most of siliceous grade inorganic salts in ash content；
3）By the substance release in cooker out, and in being transferred to mill grind 30min；Part macromolecular is cracked with Mechanical Method
Between it is chemical bonded, be fully contacted beneficial to next step enzyme system and material；
4）Step 3）Gained slurry is placed in enzymatic vessel, and the water of 6 times of slurry quality is added in enzymatic vessel, adds compound protein
Enzyme（500U/ kilogram of butt bagasse）, intermittently 1 minute, inert gas is passed through, is digested 40 minutes；It is subsequently added normal temperature type α-shallow lake
Powder enzyme（400U/ kilogram of butt bagasse）, digest 30 minutes；Then pass to steam and be warming up to 65 DEG C, be incubated 12 minutes inactivators
Activity；
Wherein, compound protease is made up of the alkali protease for possessing endopeptidase activity and the Proteinase K for possessing peptide ending enzyme activity,
It is 1 to possess the alkali protease of endopeptidase activity in compound protease with the ratio of the Proteinase K for possessing peptide ending enzyme activity: 1；
The compound protease can effectively hydrolyzing protein at normal temperatures；
Normal temperature type AMS is by microwave induced gained variation bacillus licheniformis secretion gained AMS；Microwave induced gained
The obtaining step of variation bacillus licheniformis is specially：The nutrient solution of bacillus licheniformis is placed in into microwave generator, is arranged micro-
Wave power is 900W, and pulse frequency is 2300MHz, microwave treatment 20s, cools down 20s, reciprocal 30 times according to this；After microwave treatment
Nutrient solution be coated on solid medium, under the conditions of 30 DEG C cultivate 1-2 days, by the bacterium colony for surviving screen four plants of normal temperature
The dissociant of the high bacillus licheniformis of lower alpha-amylase activity.Select alpha-amylase activity highest lichens gemma under normal temperature
The dissociant Amplification Culture of bacillus, so as to obtain normal temperature type AMS；Normal temperature type AMS is high at a temperature of 22-35 DEG C
Efficient hydrolysis starch, it is not necessary to as the thermal-stable α-amylase for adopting mostly at present needs high temperature（80-90℃）Condition, thus subtract
Energy consumption is lacked and has also reduced the requirement to equipment, while greatly reducing the generation of side reaction；Herein can be mildly with enzyme process
Protein, Starch Hydrolysis are entered in filtrate, so as to successfully remove into the peptides of small molecule, amino acid, maltose, glucose etc.
Protein and starch；
5）Go out after enzyme, separation of solid and liquid obtains filter residue one and filtrate one by the substance release in enzymatic vessel out；
6）Gained filter residue one is washed several times with 2% potassium hydroxide solution, and separation of solid and liquid obtains filter residue two and filtrate two；
7）Acetic acid,diluted is added in filtrate two, pH is adjusted to 5, after stirring, low temperature（6℃）Lower standing, stablizes 4 hours, separates out
Precipitation, separation of solid and liquid obtain filter residue three and filtrate three；
8）The potassium hydroxide solution dissolving for Jia 2% in filter residue three, then acid out again；Repeat 2-3 time, separation of solid and liquid obtains four He of filter residue
Filtrate four；
9）Filter residue four is spray-dried, crushing, obtains water-soluble dietary fiber（Water soluble pentosan）, high purity 98%；Spraying is dry
Dry room temperature is less than 60 DEG C；
10）The 95% of 4 times of volumes ethanol is added in filtrate three, after stirring, is stablized 2 hours, separated out and precipitate, separation of solid and liquid,
Obtain filter residue five and filtrate five；
11）Filter residue Five Classics heated-air drying（Less than 60 DEG C）, crush, obtain part Water insoluble dietary fiber（Water-insoluble half fiber
Element）, high purity 95%；Two Jing fluidized bed dryings of filter residue（Less than 60 DEG C）, crush, obtain another part Water insoluble dietary fiber
（Cellulose and lignin）High purity 96%.
Embodiment 2
A kind of utilization bagasse produces the water-soluble method with Water insoluble dietary fiber, including step simultaneously：
1）Bagasse impurity elimination（Reject big material）After insert cooker；
2）The water of 12 times of bagasse quality is added in cooker, adds ammoniacal liquor to alkali lye mass concentration to be 5%；It is passed through steam extremely
In cooker, stop logical steam after being warming up to 85 DEG C, inert nitrogen gas are passed through in cooker, air in cooker is arranged
Only, seal；Then intermittence is passed through steam and inert gas into cooker, maintaining in cooker temperature as 90 DEG C and
Pressure is 0.6MPa, heat-insulation pressure keeping 60 minutes；
In the step, adopt relatively low boiling temperature to avoid decomposition reaction, the dehydration of each component（Coking）Reaction, peeling reaction
Deng using elevated pressures（By being passed through inert gas realization）Ensure that alkali lye, to the effective infiltration between bagasse cell membrane, is adopted
Use inert environments（It is passed through inert gas realization）Avoid oxidation reaction, the esterification of acetyl group of each component molecular radical
Deng so as to ensure that integrality, high yield pulp1 and the high-quality of each material；This step diluted alkaline can extract hemicellulose simultaneously
Extract the most of siliceous grade inorganic salts in ash content；
3）By the substance release in cooker out, and in being transferred to colloid mill grind 30min；Big point of part is cracked with Mechanical Method
It is chemical bonded between son, it is fully contacted beneficial to next step enzyme system and material；
4）Step 3）Gained slurry is placed in enzymatic vessel, and the water of 4 times of slurry quality is added in enzymatic vessel, adds compound protein
Enzyme（600U/ kilogram of butt bagasse）, intermittently 1 minute, inert gas is passed through, is digested 30 minutes；It is subsequently added normal temperature type α-shallow lake
Powder enzyme（500U/ kilogram of butt bagasse）, digest 25 minutes；Then pass to steam and be warming up to 70 DEG C, be incubated 10 minutes inactivators
Activity；
Wherein, compound protease is made up of the alkali protease for possessing endopeptidase activity and the Proteinase K for possessing peptide ending enzyme activity,
It is 1 to possess the alkali protease of endopeptidase activity in compound protease with the ratio of the Proteinase K for possessing peptide ending enzyme activity: 2；
The compound protease can effectively hydrolyzing protein at normal temperatures；
Normal temperature type AMS is by microwave induced gained variation bacillus licheniformis secretion gained AMS；Microwave induced gained
The obtaining step of variation bacillus licheniformis is specially：The nutrient solution of bacillus licheniformis is placed in into microwave generator, is arranged micro-
Wave power is 900W, and pulse frequency is 2300MHz, microwave treatment 20s, cools down 20s, reciprocal 30 times according to this；After microwave treatment
Nutrient solution be coated on solid medium, under the conditions of 30 DEG C cultivate 1-2 days, by the bacterium colony for surviving screen four plants of normal temperature
The dissociant of the high bacillus licheniformis of lower alpha-amylase activity.Select alpha-amylase activity highest lichens gemma under normal temperature
The dissociant Amplification Culture of bacillus, so as to obtain normal temperature type AMS；Normal temperature type AMS is high at a temperature of 22-35 DEG C
Efficient hydrolysis starch, it is not necessary to as the thermal-stable α-amylase for adopting mostly at present needs high temperature（80-90℃）Condition, thus subtract
Energy consumption is lacked and has also reduced the requirement to equipment, while greatly reducing the generation of side reaction；Herein can be mildly with enzyme process
Protein, Starch Hydrolysis are entered in filtrate, so as to successfully remove into the peptides of small molecule, amino acid, maltose, glucose etc.
Protein and starch；
5）Go out after enzyme, separation of solid and liquid obtains filter residue one and filtrate one by the substance release in enzymatic vessel out；
6）Gained filter residue one is washed several times with 3% ammonia spirit, separation of solid and liquid, and centralized collection washing lotion is to filtrate one, must filter
Slag two and filtrate two；
7）Watery hydrochloric acid is added in filtrate two, pH is adjusted to 4.5, after stirring, low temperature（5℃）Lower standing, stablizes 4 hours, analysis
Go out precipitation, separation of solid and liquid obtains filter residue three and filtrate three；
8）The ammonia spirit dissolving for Jia 3% in filter residue three, then acid out again；Repeat 3 times, separation of solid and liquid obtains filter residue four and filtrate
Four；
9）Four Jing heated-air dryings of filter residue, crushing, obtain water-soluble dietary fiber（Water soluble pentosan）, high purity 97%；It is dried temperature
Degree is less than 60 DEG C；
10）The 95% of 5 times of volumes ethanol is added in filtrate three, after stirring, is stablized 2 hours, separated out and precipitate, separation of solid and liquid,
Obtain filter residue five and filtrate five；
11）Filter residue Five Classics heated-air drying（Less than 60 DEG C）, crush, obtain part Water insoluble dietary fiber（Water-insoluble half fiber
Element）, high purity 96%；Two Jing fluidized bed dryings of filter residue（Less than 60 DEG C）, crush, obtain another part Water insoluble dietary fiber
（Cellulose and lignin）, high purity 97%.
Claims (8)
1. a kind of utilization bagasse produces the water-soluble method with Water insoluble dietary fiber simultaneously, it is characterised in that including step
Suddenly：
1）Cooker is inserted after bagasse impurity elimination；
2）The water of bagasse quality 1-20 times is added in cooker, adds alkali to alkali lye mass concentration to be 1-10%；It is passed through steam
Be warming up to cooker after 60-100 DEG C and stop logical steam, inert gas is passed through in cooker, air in cooker is arranged
Only；Then intermittence is passed through steam and inert gas into cooker, maintaining in cooker temperature as 60-100 DEG C and
Pressure is 0.1-1MPa, heat-insulation pressure keeping 10-120 minutes；
3）By the substance release in cooker out, and in being transferred to grinding mill grind；
4）Step 3）Gained slurry is placed in enzymatic vessel, and the water of slurry quality 1-10 times is added in enzymatic vessel, adds compound
Protease, interval 1-3 minute, is passed through inert gas, digests 10-80 minutes；Normal temperature type AMS is subsequently added, 10- is digested
80 minutes；Then pass to steam and be warming up to 60-70 DEG C, be incubated the activity of 2-20 minute inactivators；The compound protease is by having
The alkali protease of standby endopeptidase activity and the Proteinase K for possessing peptide ending enzyme activity are constituted；The normal temperature type AMS is by micro-
Ripple induction gained variation bacillus licheniformis secretion gained AMS, the preference temperature of the normal temperature type AMS is 22-
35℃；
5）Go out after enzyme, separation of solid and liquid obtains filter residue one and filtrate one by the substance release in enzymatic vessel out；
6）The gained sig water of filter residue one is washed several times, separation of solid and liquid, and centralized collection washing lotion is to filtrate one, obtains two He of filter residue
Filtrate two；
7）Diluted acid being added in filtrate two, pH being adjusted to 4-6, after stirring, stable 1-5 hours, separate out precipitation, and separation of solid and liquid is obtained
Filter residue three and filtrate three；
8）The liquid that adds dilute alkali in filter residue three dissolves, then acid out again；Repeat 2-3 time, separation of solid and liquid obtains filter residue four and filtrate four；
9）Four drying of filter residue, crushing, obtain water-soluble dietary fiber；
10）The ethanol of 1-10 times of volume is added in filtrate three, after stirring, stable 1-3 hours are separated out and precipitated, separation of solid and liquid,
Obtain filter residue five and filtrate five；
11）Filter residue five and two drying of filter residue, crushing, obtain Water insoluble dietary fiber.
2. the water-soluble method with Water insoluble dietary fiber, its feature are produced simultaneously using bagasse as claimed in claim 1
It is, step 4）In, the obtaining step of the microwave induced gained variation bacillus licheniformis is specially：By bacillus licheniformis
Nutrient solution be placed in microwave generator, setting microwave power is 850-950W, and pulse frequency is 2300MHz, microwave treatment 20s,
Cooling 20s, it is reciprocal 25-35 time according to this；Nutrient solution after microwave treatment is coated on solid medium, is cultivated under the conditions of 30 DEG C
1-2 days, by the dissociant that the high bacillus licheniformis of alpha-amylase activity under four plants of normal temperature is screened in the bacterium colony for surviving.
3. the water-soluble method with Water insoluble dietary fiber, its feature are produced simultaneously using bagasse as claimed in claim 2
It is：The dissociant Amplification Culture of alpha-amylase activity highest bacillus licheniformis under normal temperature is selected, it is described so as to obtain
Normal temperature type AMS.
4. the water-soluble method with Water insoluble dietary fiber is produced simultaneously using bagasse, which special as claimed in claim 1 or 2
Levy and be：Possess the alkali protease and the Proteinase K for possessing peptide ending enzyme activity of endopeptidase activity in the compound protease
Ratio is 1:1-3；The addition of the compound protease meets every kilogram of butt bagasse 400-800U, the normal temperature type α-shallow lake
The addition of powder enzyme meets every kilogram of butt bagasse 300-700U.
5. the water-soluble method with Water insoluble dietary fiber, its feature are produced simultaneously using bagasse as claimed in claim 1
It is：The alkali is NaOH, potassium hydroxide, ammoniacal liquor or calcium hydroxide；The acid is hydrochloric acid, nitric acid or acetic acid.
6. the water-soluble method with Water insoluble dietary fiber, its feature are produced simultaneously using bagasse as claimed in claim 1
It is：The inert gas is nitrogen, helium or argon gas.
7. the water-soluble method with Water insoluble dietary fiber, its feature are produced simultaneously using bagasse as claimed in claim 1
It is：Step 9）, step 11）Described in be dried as spray drying, heated-air drying, fluidized bed drying or freeze-drying；It is dried temperature
Degree is less than 120 DEG C.
8. the water-soluble method with Water insoluble dietary fiber, its feature are produced simultaneously using bagasse as claimed in claim 1
It is：Step 10）In the volume fraction of ethanol used be 95%.
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| CN110074418A (en) * | 2019-05-21 | 2019-08-02 | 山东探克生物科技股份有限公司 | A kind of extracting method of seaweed diet fiber |
| CN110574934A (en) * | 2019-10-16 | 2019-12-17 | 广西马中粮油有限公司 | Method for producing dietary fiber by using rice hull as raw material |
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| CN113016950A (en) * | 2021-04-20 | 2021-06-25 | 哈尔滨求真生物科技有限公司 | Series products prepared from bagasse, preparation method and comprehensive utilization method of bagasse |
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| CN110574934A (en) * | 2019-10-16 | 2019-12-17 | 广西马中粮油有限公司 | Method for producing dietary fiber by using rice hull as raw material |
| CN110652014A (en) * | 2019-10-16 | 2020-01-07 | 梁乐 | Method for preparing dietary fiber from zedoary turmeric leaves |
| CN113016950A (en) * | 2021-04-20 | 2021-06-25 | 哈尔滨求真生物科技有限公司 | Series products prepared from bagasse, preparation method and comprehensive utilization method of bagasse |
| CN113016950B (en) * | 2021-04-20 | 2024-03-05 | 中粮崇左糖业有限公司 | Series products prepared from bagasse, preparation method and bagasse comprehensive utilization method |
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