CN106509914A - Method for preparing dietary fibers by use of crop straws - Google Patents
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Abstract
The invention discloses a method for preparing dietary fibers by use of crop straws and belongs to the biotechnology field. According to the method disclosed by the invention, a conventional steam explosion separation technology is improved; in the process, low-temperature and high-pressure conditions are adopted, and special bio-enzyme is applied, so that high efficiency, high quality and high benefits of straw utilization are ensured. The yield of dietary fibers prepared by use of various straws is high, and the prepared dietary fibers are high in purity. The process for preparing the dietary fibers is simple, less in investment, short in period and easy to popularize.
Description
Technical field
The present invention relates to biological technical field, the method that more particularly to a kind of crop material prepares dietary fiber.
Background technology
Dietary fiber refers mainly to come from the cellulose of plant cell wall, hemicellulose, lignin and resin, pectin etc.
It is difficult digested composition.Dietary fiber is that health diet is indispensable, is played the part of emphatically in terms of digestive system health is kept
The role for wanting.
China there are about 1,000,000,000 people and answer complementary diets fiber.By 6 grams of calculating are supplemented for each person every day, then 2,200,000 tons are needed every year
Dietary fiber.And the annual production of the present dietary fiber of China is less than 100,000 tons.Therefore, the potential market of China's dietary fiber is very
Greatly, market prospects are boundless, it would be highly desirable to develop.
At present, wheat bran, corn bran, rice bran, bean dregs, pericarp and konjaku etc. is adopted both at home and abroad to be prepared for raw material
Dietary fiber；But compared with the dietary fiber in stalk source, the water imbibition and rising property of profit of its product is weaker.
China every year can more than 900,000,000 tons of harvesting crops stalk.According to surveying and determination, the crude fibre that crop material contains（Including cellulose,
Hemicellulose and lignin）Total amount accounts for the mass fraction of dry weight and averagely reaches 75-85%, is the excellent former material for preparing dietary fiber
Material.
The stalk includes the crops such as wheat, corn, paddy rice, cotton, Chinese sorghum, soybean, peanut, potato class, melon and lucerne
Remaining stem, leaf, skin after the herbage harvesting seed such as Mu, prairie milk vetch（Shell）, climing rattan（Seedling）Deng and sugarcane（Slag）Corncob, bamboo
Son, reed etc..
In recent years, many research works have been carried out to preparing the technique of dietary fiber, equipment, method etc. using stalk both at home and abroad
Make, such as Chinese patent（105,029,452 105695325 A etc. of A, CN of CN）Disclose in succession and extracted using wheat, maize straw
The method of dietary fiber.But not yet there are process is simple, easy to spread, small investment, the life of the stalk dietary fiber of cycle is short at present
Product technology.
The content of the invention
In order to make up the deficiencies in the prior art, the invention provides a kind of method that crop material prepares dietary fiber.
The technical scheme is that：
A kind of method that crop material prepares dietary fiber, including step：
1）Stalk Jing impurity elimination, dedusting, rub silk, segment, insert cooker after washing；
2）The water of stalk quality 1-20 times is added in cooker, adds alkali to alkali lye mass concentration to be 1-10%；It is passed through steam extremely
60-100 DEG C is warming up in cooker, inert gas is passed through in cooker, by air emptying in cooker；Then it is intermittent to
Steam and inert gas is passed through in cooker, is 60-100 DEG C, pressure as 0.1-0.3MPa to maintain temperature in cooker, protect
Warm pressurize 10-120 minutes；
3）By the substance release in cooker out, and grind in being transferred to grinding mill；
4）Separation of solid and liquid, obtains filter residue one and filtrate one；
5）Filter residue one is inserted into enzymatic vessel, the water of one mass 1-10 times of filter residue is added in enzymatic vessel, with dilute acid for adjusting pH to 4-
8, compound protease is added, interval 1-3 minute, inert gas is passed through, is digested 10-80 minutes；It is subsequently added normal temperature type alphalise starch
Enzyme, digests 10-80 minutes；Then pass to steam and be warming up to 60-70 DEG C, be incubated the activity of 2-20 minute inactivators；It is described compound
Protease is made up of the alkali protease for possessing endopeptidase activity and the Proteinase K for possessing peptide ending enzyme activity；The normal temperature type α-
Amylase by microwave induced gained variation bacillus licheniformis secretion gained AMS, the normal temperature type AMS it is suitable
Temperature is 22-35 DEG C；
6）Go out after enzyme, separation of solid and liquid obtains filter residue two and filtrate two by the substance release in enzymatic vessel out；
7）Gained filter residue two is washed with water several times, separation of solid and liquid, and centralized collection filtrate, obtains filter residue three and filtrate three；
8）Three drying of filter residue, crushing, obtain final product the composite fibre containing cellulose and lignin；
9）The ethanol of one volume 1-10 times of filtrate is added in filtrate one, and after stirring, stable 1-3 hours, separate to obtain filter residue four and filtrate
Four；
10）Filter residue four is dissolved using sig water, is subsequently adding the ethanol of sig water volume 1-10 times, stable to precipitation analysis after stirring
Go out completely, precipitation and separation and clear liquid；
11）Step 10）Gained precipitation repeat step 10）Operation several times, be finally recovered to obtain filter residue five and filtrate five；
12）Filter residue Five Classics drying and crushing obtains hemicellulose finished product；
13）Hemicellulose finished product and step 8）Composite fibre of the gained containing cellulose and lignin is mixed, and obtains final product dietary fiber
Finished product.
Preferably, step 5）In, the obtaining step of the microwave induced gained variation bacillus licheniformis is concrete
For：The nutrient solution of bacillus licheniformis is placed in into microwave generator, setting microwave power is 850-950W, pulse frequency is
2300MHz, microwave treatment 20s cool down 20s, reciprocal 25-35 time according to this；Nutrient solution after microwave treatment is coated on into solid training
On foster base, cultivate 1-2 days under the conditions of 30 DEG C, by the high ground of alpha-amylase activity under four plants of normal temperature of screening in the bacterium colony for surviving
The dissociant of clothing bacillus.
Further, the dissociant Amplification Culture of alpha-amylase activity highest bacillus licheniformis under normal temperature is selected,
So as to obtain the normal temperature type AMS.The normal temperature type AMS obtained using the method, at normal temperatures can efficient enzyme
Solution starch, had both reduced energy consumption, turn avoid the generation of side reaction.
Preferably, possess the alkali protease of endopeptidase activity in the compound protease and possess peptide ending enzyme work
Property Proteinase K ratio be 1:1-3；The addition of the compound protease meets every kilogram of butt stalk 400-800U, institute
The addition for stating normal temperature type AMS meets every kilogram of butt stalk 300-700U.
Preferably, the alkali is NaOH, potassium hydroxide, ammoniacal liquor or calcium hydroxide.
Preferably, the inert gas is nitrogen, helium or argon gas.Preferably nitrogen, nitrogen low cost.
Preferably, step 8）, step 12）Described in be dried for spray drying, heated-air drying, fluidized bed drying or
Freeze-drying；Baking temperature is less than 120 DEG C.
Preferably, step 9）, step 10）In the volume fraction of ethanol used be 95%.
Beneficial effects of the present invention are：
The present invention is improved to conventional steam explosion isolation technics, employs low temperature, condition of high voltage, and apply spy in technique
Different biology enzyme, it is ensured that the present invention is to the high efficiency of straw utilization, high-quality, high yield.The present invention utilizes various stalk systems
Standby dietary fiber yield is high, and the purity of gained dietary fiber is high.
The present invention, can be with newly-built when implementing, it is also possible to makes full use of numerous paper pulp, wood sugars for or frequently facing bankruptcy
Production enterprise（Especially middle-size and small-size paper pulp, wood sugar enterprise）, after its equipment, workshop are slightly transformed by produce it is qualified
Dietary fiber；Put into little, cycle is short, easily promote.
Specific embodiment
Embodiment 1
A kind of method that crop material prepares dietary fiber, including step：
1）Wheat stalk Jing impurity elimination, dedusting, rub silk, segment, insert cooker after washing；
2）The water of 10 times of stalk quality is added in cooker, adds ammoniacal liquor to alkali lye mass concentration to be 6%；Steam is passed through to steaming
Boil, inert gas is passed through in cooker, by air emptying in cooker；Then intermittence is to cooker
Steam and inert gas is inside passed through, is 95 DEG C, pressure as 0.3MPa to maintain temperature in cooker, heat-insulation pressure keeping 60 minutes；
Intermittence is passed through inert gas can also be such that stalk is uniformly distributed in cooker；
In the step, adopt relatively low boiling temperature to avoid decomposition reaction, the dehydration of each component（Coking）Reaction, peeling reaction
Deng using elevated pressures（By being passed through inert gas realization）Ensure that alkali lye, to the effective infiltration between stalk cell wall, is adopted
Inert environments（It is passed through inert gas realization）Oxidation reaction, esterification of acetyl group of each component molecular radical etc. is avoided,
So as to ensure that integrality, high yield pulp1 and the high-quality of each material；This step diluted alkaline can extract hemicellulose and take out
Most of siliceous grade inorganic salts in proposition ash content；
3）By the substance release in cooker out, and be transferred to grinding mill（Mill or colloid mill etc.）Middle grinding 20 minutes；With
Mechanical Method cracks chemical bonded between the macromolecular of part, is fully contacted beneficial to next step enzyme system and material.
4）Separation of solid and liquid, obtains filter residue one and filtrate one；
5）Filter residue one is inserted into enzymatic vessel, the water of 5 times of one mass of filter residue is added in enzymatic vessel, pH is adjusted to 7 with acetic acid,diluted, plus
Enter compound protease（500U/ kilogram of butt stalk）, intermittently 1 minute, it is passed through nitrogen 1 minute, digests 40 minutes；Often it is subsequently added
Warm type AMS（400U/ kilogram of butt stalk）, digest 30 minutes；Then pass to steam and be warming up to 65 DEG C, be incubated 12 minutes
The activity of inactivator；
Wherein, compound protease is made up of the alkali protease for possessing endopeptidase activity and the Proteinase K for possessing peptide ending enzyme activity,
It is 1 to possess the alkali protease of endopeptidase activity in compound protease with the ratio of the Proteinase K for possessing peptide ending enzyme activity: 1；
The compound protease can effectively hydrolyzing protein at normal temperatures；
Normal temperature type AMS is by microwave induced gained variation bacillus licheniformis secretion gained AMS；Microwave induced gained
The obtaining step of variation bacillus licheniformis is specially：The nutrient solution of bacillus licheniformis is placed in into microwave generator, is arranged micro-
Wave power is 900W, and pulse frequency is 2300MHz, microwave treatment 20s, cools down 20s, reciprocal 30 times according to this；After microwave treatment
Nutrient solution be coated on solid medium, under the conditions of 30 DEG C cultivate 1-2 days, by the bacterium colony for surviving screen four plants of normal temperature
The dissociant of the high bacillus licheniformis of lower alpha-amylase activity.Select alpha-amylase activity highest lichens gemma under normal temperature
The dissociant Amplification Culture of bacillus, so that obtain normal temperature type AMS；Normal temperature type AMS is high at a temperature of 22-35 DEG C
Efficient hydrolysis starch, it is not necessary to as the thermal-stable α-amylase for adopting mostly at present needs high temperature（80-90℃）Condition, thus subtract
Energy consumption is lacked and has also reduced the requirement to equipment, while greatly reducing the generation of side reaction；Herein can be mildly with enzyme process
Protein, Starch Hydrolysis are entered in filtrate, so as to successfully remove into the peptides of small molecule, amino acid, maltose, glucose etc.
Protein and starch；To ensure the purity of dietary fiber.
6）Go out after enzyme, using filter press press filtration, separation of solid and liquid, obtain two He of filter residue by the substance release in enzymatic vessel out
Filtrate two；
7）Gained filter residue two is washed twice with water, separation of solid and liquid, and centralized collection filtrate, obtains filter residue three and filtrate three；
8）Three Jing heated-air dryings of filter residue（Temperature is less than 120 DEG C）, crush, obtain final product the composite fibre containing cellulose and lignin；
9）The 95% of 5 times of one volume of filtrate ethanol is added in filtrate one, is stirred 15 minutes, is stablized 1.5 hours, separate to obtain filter residue
Four and filtrate four；
10）Filter residue four is dissolved using weak aqua ammonia, is subsequently adding the 95% of 5 times of weak aqua ammonia volume ethanol, stable to precipitation after stirring
Separate out complete, precipitation and separation and clear liquid；
11）Step 10）Gained precipitation repeat step 10）Operation twice, be finally recovered to obtain filter residue five and filtrate five；
12）Filter residue Five Classics heated-air drying（Temperature is less than 120 DEG C）, crush to obtain hemicellulose finished product；
13）Hemicellulose finished product and step 8）Composite fibre of the gained containing cellulose and lignin is mixed, and obtains final product dietary fiber
Finished product.
According to standard GB/T/T 5009.88 2008（The measure of dietary fiber in food）Detected.As a result table
It is bright, the dietary fiber purity 90% of the dietary fiber yield 85%, wheat stalk of above-mentioned wheat stalk；Remaining indices meets
Dietary fiber Standard.
In addition, the recyclable recycling of ethanol in whole technique.
Embodiment 2
A kind of method that crop material prepares dietary fiber, including step：
1）Maize straw Jing impurity elimination, dedusting, rub silk, segment, insert cooker after washing；
2）The water of 8 times of stalk quality is added in cooker, adds potassium hydroxide to alkali lye mass concentration to be 8%；It is passed through steam extremely
90 DEG C are warming up in cooker, inert nitrogen gas is passed through in cooker, by air emptying in cooker；Then it is intermittent to
Steam and inert nitrogen gas being passed through in cooker, being 90 DEG C, pressure as 0.2MPa to maintain temperature in cooker, insulation is protected
Pressure 90 minutes；Intermittence is passed through inert gas can also be such that stalk is uniformly distributed in cooker；
In the step, adopt relatively low boiling temperature to avoid decomposition reaction, the dehydration of each component（Coking）Reaction, peeling reaction
Deng using elevated pressures（By being passed through inert gas realization）Ensure that alkali lye, to the effective infiltration between stalk cell wall, is adopted
Inert environments（It is passed through inert gas realization）Oxidation reaction, esterification of acetyl group of each component molecular radical etc. is avoided,
So as to ensure integrality, high yield pulp1 and the high-quality of each material；This step diluted alkaline can extract hemicellulose and take out
Most of siliceous grade inorganic salts in proposition ash content；
3）By the substance release in cooker out, and be transferred to grinding mill（Mill or colloid mill etc.）Middle grinding 20 minutes；With
Mechanical Method cracks chemical bonded between the macromolecular of part, is fully contacted beneficial to next step enzyme system and material.
4）Separation of solid and liquid, obtains filter residue one and filtrate one；
5）Filter residue one is inserted into enzymatic vessel, the water of 5 times of one mass of filter residue is added in enzymatic vessel, pH is adjusted to 7.2 with dust technology,
Add compound protease（600U/ kilogram of butt stalk）, intermittently 1 minute, it is passed through nitrogen 1 minute, digests 50 minutes；It is subsequently added
Normal temperature type AMS（500U/ kilogram of butt stalk）, digest 45 minutes；Then pass to steam and be warming up to 70 DEG C, be incubated 12 points
The activity of clock inactivator；
Wherein, compound protease is made up of the alkali protease for possessing endopeptidase activity and the Proteinase K for possessing peptide ending enzyme activity,
It is 1 to possess the alkali protease of endopeptidase activity in compound protease with the ratio of the Proteinase K for possessing peptide ending enzyme activity: 2；
The compound protease can be in high efficiency aminosal under normal temperature condition；Normal temperature type AMS is made a variation by microwave induced gained
Bacillus licheniformis secretion gained AMS；The obtaining step of microwave induced gained variation bacillus licheniformis is specially：Will
The nutrient solution of bacillus licheniformis is placed in microwave generator, and setting microwave power is 900W, and pulse frequency is 2300MHz, microwave
Process 20s, cooling 20s, reciprocal 30 times according to this；Nutrient solution after microwave treatment is coated on solid medium, 30 DEG C of conditions
Lower culture 1-2 days, by the change for screening the high bacillus licheniformis of alpha-amylase activity under four plants of normal temperature in the bacterium colony for surviving
Different bacterial strain.The dissociant Amplification Culture of alpha-amylase activity highest bacillus licheniformis under normal temperature is selected, so as to often obtain
Warm type AMS；Normal temperature type AMS expeditiously hydrolysis starch at a temperature of 22-35 DEG C, it is not necessary to as adopting mostly at present
Thermal-stable α-amylase need high temperature（80-90℃）Condition, thus reduce energy consumption and also reduce the requirement to equipment, while
Greatly reduce the generation of side reaction；Herein with enzyme process can mildly by protein, Starch Hydrolysis into small molecule peptides, ammonia
Base acid, maltose, glucose etc. are entered in filtrate, so as to successful deproteination matter and starch；To ensure the purity of dietary fiber.
6）Go out after enzyme, using filter press press filtration, separation of solid and liquid, obtain two He of filter residue by the substance release in enzymatic vessel out
Filtrate two；
7）Gained filter residue two is washed twice with water, separation of solid and liquid, and centralized collection filtrate, obtains filter residue three and filtrate three；
8）Three Jing fluidized bed dryings of filter residue（Temperature is less than 120 DEG C）, crush, obtain final product mixing containing cellulose and lignin fine
Dimension；
9）The 95% of 6 times of one volume of filtrate ethanol is added in filtrate one, is stirred 10 minutes, is stablized 2 hours, separate to obtain filter residue four
With filtrate four；
10）Filter residue four is dissolved using dilute potassium hydroxide solution, is subsequently adding the 95% of 5 times of dilute potassium hydroxide solution volume ethanol,
After stirring it is stable to Precipitation completely, precipitation and separation and clear liquid；
11）Step 10）Gained precipitation repeat step 10）Operation twice, be finally recovered to obtain filter residue five and filtrate five；
12）Filter residue Five Classics fluidized bed drying（Temperature is less than 120 DEG C）, crush to obtain hemicellulose finished product；
13）Hemicellulose finished product and step 8）Composite fibre of the gained containing cellulose and lignin is mixed, and obtains final product dietary fiber
Finished product.
According to standard GB/T/T 5009.88 2008（The measure of dietary fiber in food）Detected.As a result table
It is bright, the dietary fiber purity 92% of the dietary fiber yield 86%, maize straw of above-mentioned maize straw；Remaining indices meets
Dietary fiber Standard.
In addition, the recyclable recycling of ethanol in whole technique.
Embodiment 3
A kind of method that crop material prepares dietary fiber, including step：
1）Reed Jing impurity elimination, dedusting, rub silk, segment, insert cooker after washing；
2）The water of 12 times of reed quality is added in cooker, adds ammoniacal liquor to alkali lye mass concentration to be 6%；Steam is passed through to steaming
Boil, inert gas is passed through in cooker, by air emptying in cooker；Then intermittence is to boiling
Steam and inert gas is passed through in tank, is 92.5 DEG C, pressure as 0.4MPa to maintain temperature in cooker, 70 points of heat-insulation pressure keeping
Clock；Intermittence is passed through inert gas can also be such that material is uniformly distributed in cooker；
In the step, adopt relatively low boiling temperature to avoid decomposition reaction, the dehydration of each component（Coking）Reaction, peeling reaction
Deng using elevated pressures（By being passed through inert gas realization）Ensure that alkali lye, to the effective infiltration between reed cell membrane, is adopted
Inert environments（It is passed through inert gas realization）Oxidation reaction, esterification of acetyl group of each component molecular radical etc. is avoided,
So as to ensure that integrality, high yield pulp1 and the high-quality of each material；This step diluted alkaline can extract hemicellulose and take out
Most of siliceous grade inorganic salts in proposition ash content；
3）By the substance release in cooker out, and be transferred to grinding mill（Mill or colloid mill etc.）Middle grinding 25 minutes；With
Mechanical Method cracks chemical bonded between the macromolecular of part, is fully contacted beneficial to next step enzyme system and material.
4）Separation of solid and liquid, obtains filter residue one and filtrate one；
5）Filter residue one is inserted into enzymatic vessel, the water of 6 times of one mass of filter residue is added in enzymatic vessel, pH is adjusted to 7.1 with acetic acid,diluted,
Add compound protease（550U/ kilogram of butt stalk）, intermittently 1 minute, it is passed through nitrogen 1 minute, digests 40 minutes；It is subsequently added
Normal temperature type AMS（450U/ kilogram of butt stalk）, digest 30 minutes；Then pass to steam and be warming up to 65 DEG C, be incubated 14 points
The activity of clock inactivator；
Wherein, compound protease is made up of the alkali protease for possessing endopeptidase activity and the Proteinase K for possessing peptide ending enzyme activity,
It is 1 to possess the alkali protease of endopeptidase activity in compound protease with the ratio of the Proteinase K for possessing peptide ending enzyme activity:
1.5；The compound protease can effectively hydrolyzing protein at normal temperatures；
Normal temperature type AMS is by microwave induced gained variation bacillus licheniformis secretion gained AMS；Microwave induced gained
The obtaining step of variation bacillus licheniformis is specially：The nutrient solution of bacillus licheniformis is placed in into microwave generator, is arranged micro-
Wave power is 900W, and pulse frequency is 2300MHz, microwave treatment 20s, cools down 20s, reciprocal 30 times according to this；After microwave treatment
Nutrient solution be coated on solid medium, under the conditions of 30 DEG C cultivate 1-2 days, by the bacterium colony for surviving screen four plants of normal temperature
The dissociant of the high bacillus licheniformis of lower alpha-amylase activity.Select alpha-amylase activity highest lichens gemma under normal temperature
The dissociant Amplification Culture of bacillus, so that obtain normal temperature type AMS；Normal temperature type AMS is high at a temperature of 22-35 DEG C
Efficient hydrolysis starch, it is not necessary to as the thermal-stable α-amylase for adopting mostly at present needs high temperature（80-90℃）Condition, thus subtract
Energy consumption is lacked and has also reduced the requirement to equipment, while greatly reducing the generation of side reaction；Herein can be mildly with enzyme process
Protein, Starch Hydrolysis are entered in filtrate, so as to successfully remove into the peptides of small molecule, amino acid, maltose, glucose etc.
Protein and starch；To ensure the purity of dietary fiber.
6）Go out after enzyme, using filter press press filtration, separation of solid and liquid, obtain two He of filter residue by the substance release in enzymatic vessel out
Filtrate two；
7）Gained filter residue two is washed twice with water, separation of solid and liquid, and centralized collection filtrate, obtains filter residue three and filtrate three；
8）Three Jing heated-air dryings of filter residue（Temperature is less than 120 DEG C）, crush, obtain final product the composite fibre containing cellulose and lignin；
9）The 95% of 4 times of one volume of filtrate ethanol is added in filtrate one, is stirred 12 minutes, is stablized 2 hours, separate to obtain filter residue four
With filtrate four；
10）Filter residue four is dissolved using weak aqua ammonia, is subsequently adding the 95% of 4 times of weak aqua ammonia volume ethanol, stable to precipitation after stirring
Separate out complete, precipitation and separation and clear liquid；
11）Step 10）Gained precipitation repeat step 10）Operation twice, be finally recovered to obtain filter residue five and filtrate five；
12）Filter residue Five Classics heated-air drying（Temperature is less than 120 DEG C）, crush to obtain hemicellulose finished product；
13）Hemicellulose finished product and step 8）Composite fibre of the gained containing cellulose and lignin is mixed, and obtains final product dietary fiber
Finished product.
According to standard GB/T/T 5009.88 2008（The measure of dietary fiber in food）Detected.As a result table
It is bright, the dietary fiber purity 94% of the dietary fiber yield 89%, reed of above-mentioned reed；Remaining indices meets dietary fiber
Standard.
In addition, the recyclable recycling of ethanol in whole technique.
Claims (8)
1. a kind of method that crop material prepares dietary fiber, it is characterised in that including step：
1）Stalk Jing impurity elimination, dedusting, rub silk, segment, insert cooker after washing；
2）The water of stalk quality 1-20 times is added in cooker, adds alkali to alkali lye mass concentration to be 1-10%；It is passed through steam extremely
60-100 DEG C is warming up in cooker, inert gas is passed through in cooker, by air emptying in cooker；Then it is intermittent to
Steam and inert gas is passed through in cooker, is 60-100 DEG C, pressure as 0.1-0.3MPa to maintain temperature in cooker, protect
Warm pressurize 10-120 minutes；
3）By the substance release in cooker out, and grind in being transferred to grinding mill；
4）Separation of solid and liquid, obtains filter residue one and filtrate one；
5）Filter residue one is inserted into enzymatic vessel, the water of one mass 1-10 times of filter residue is added in enzymatic vessel, with dilute acid for adjusting pH to 4-
8, compound protease is added, interval 1-3 minute, inert gas is passed through, is digested 10-80 minutes；It is subsequently added normal temperature type alphalise starch
Enzyme, digests 10-80 minutes；Then pass to steam and be warming up to 60-70 DEG C, be incubated the activity of 2-20 minute inactivators；It is described compound
Protease is made up of the alkali protease for possessing endopeptidase activity and the Proteinase K for possessing peptide ending enzyme activity；The normal temperature type α-
Amylase by microwave induced gained variation bacillus licheniformis secretion gained AMS, the normal temperature type AMS it is suitable
Temperature is 22-35 DEG C；
6）Go out after enzyme, separation of solid and liquid obtains filter residue two and filtrate two by the substance release in enzymatic vessel out；
7）Gained filter residue two is washed with water several times, separation of solid and liquid, and centralized collection filtrate, obtains filter residue three and filtrate three；
8）Three drying of filter residue, crushing, obtain final product the composite fibre containing cellulose and lignin；
9）The ethanol of one volume 1-10 times of filtrate is added in filtrate one, and after stirring, stable 1-3 hours, separate to obtain filter residue four and filtrate
Four；
10）Filter residue four is dissolved using sig water, is subsequently adding the ethanol of sig water volume 1-10 times, stable to precipitation analysis after stirring
Go out completely, precipitation and separation and clear liquid；
11）Step 10）Gained precipitation repeat step 10）Operation several times, be finally recovered to obtain filter residue five and filtrate five；
12）Filter residue Five Classics drying and crushing obtains hemicellulose finished product；
13）Hemicellulose finished product and step 8）Composite fibre of the gained containing cellulose and lignin is mixed, and obtains final product dietary fiber
Finished product.
2. the method that crop material as claimed in claim 1 prepares dietary fiber, it is characterised in that step 5）In, the microwave
The obtaining step of induction gained variation bacillus licheniformis is specially：The nutrient solution of bacillus licheniformis is placed in into microwave generation
Device, setting microwave power are 850-950W, and pulse frequency is 2300MHz, microwave treatment 20s, cool down 20s, according to this reciprocal 25-35
It is secondary；Nutrient solution after microwave treatment is coated on solid medium, is cultivated 1-2 days under the conditions of 30 DEG C, by the bacterium for surviving
Fall to screening the dissociant of the high bacillus licheniformis of alpha-amylase activity under four plants of normal temperature.
3. the method that crop material as claimed in claim 2 prepares dietary fiber, it is characterised in that：Select AMS under normal temperature
The dissociant Amplification Culture of active highest bacillus licheniformis, so that obtain the normal temperature type AMS.
4. the method that crop material as claimed in claim 1 or 2 prepares dietary fiber, it is characterised in that：The compound protease
In to possess the alkali protease of endopeptidase activity with the ratio of the Proteinase K of the activity that possesses peptide ending enzyme be 1:1-3；The compound egg
The addition of white enzyme meets every kilogram of butt stalk 400-800U, and the addition of the normal temperature type AMS meets per kilogram and does
Base stalk 300-700U.
5. the method that crop material as claimed in claim 1 prepares dietary fiber, it is characterised in that：The alkali be NaOH,
Potassium hydroxide, ammoniacal liquor or calcium hydroxide.
6. the method that crop material as claimed in claim 1 prepares dietary fiber, it is characterised in that：The inert gas is nitrogen
Gas, helium or argon gas.
7. the method that crop material as claimed in claim 1 prepares dietary fiber, it is characterised in that：Step 8）, step 12）Middle institute
State and be dried as spray drying, heated-air drying, fluidized bed drying or freeze-drying；Baking temperature is less than 120 DEG C.
8. the method that crop material as claimed in claim 1 prepares dietary fiber, it is characterised in that：Step 9）, step 10）Middle institute
It is 95% with the volume fraction of ethanol.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN106962949A (en) * | 2017-05-18 | 2017-07-21 | 成都衔石科技有限公司 | A kind of wash-off method of the stalk dietary fiber based on alkali cleaning |
| CN107125774A (en) * | 2017-05-18 | 2017-09-05 | 成都衔石科技有限公司 | A kind of alkali cleaning agent of stalk dietary fiber |
| CN107411105A (en) * | 2017-05-18 | 2017-12-01 | 成都衔石科技有限公司 | A kind of wash-off method of the stalk dietary fiber based on pickling |
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