CN107692243A - The preparation method and sugarcane dietary fiber of a kind of sugarcane dietary fiber - Google Patents

The preparation method and sugarcane dietary fiber of a kind of sugarcane dietary fiber Download PDF

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Publication number
CN107692243A
CN107692243A CN201710664917.5A CN201710664917A CN107692243A CN 107692243 A CN107692243 A CN 107692243A CN 201710664917 A CN201710664917 A CN 201710664917A CN 107692243 A CN107692243 A CN 107692243A
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mass ratio
dietary fiber
filter residue
sugarcane
water
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刘竞元
杨映宁
刘潇阳
陈朝银
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Yunnan Bowel And Health Polytron Technologies Inc
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Yunnan Bowel And Health Polytron Technologies Inc
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/20Reducing nutritive value; Dietetic products with reduced nutritive value
    • A23L33/21Addition of substantially indigestible substances, e.g. dietary fibres
    • A23L33/22Comminuted fibrous parts of plants, e.g. bagasse or pulp
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/06Enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/10General methods of cooking foods, e.g. by roasting or frying
    • A23L5/13General methods of cooking foods, e.g. by roasting or frying using water or steam
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/30Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
    • A23L5/32Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation using phonon wave energy, e.g. sound or ultrasonic waves
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Microbiology (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

The invention discloses a kind of preparation method of sugarcane dietary fiber, including pre-treatment, semi-finished product are prepared, purified, post processing.Also disclose the sugarcane dietary fiber that a kind of preparation method by the sugarcane dietary fiber is prepared.The method of the present invention is in preceding processing by the way of thermophilic digestion processing is carried out to raw material, steam explosion is handled, supersound process is combined, raw material mix tissue can effectively be destroyed, its inclusion is set fully to expose, and combine removing impurities and handle, further reduce non-dietary fiber component in raw material, raw material after pre-treatment not only contributes to the hydrolysis result of the complex enzyme of reinforcing fiber element enzyme or cellulase and hemicellulase, make enzymolysis more fully so as to improve manufactured goods purity, and the palatability of manufactured goods can be effectively improved;Purified again after enzymolysis, further improve purity, manufactured goods have the advantages of purity is high;The sugarcane dietary fiber of the present invention also has the advantages of fiber diameter is small, moisture holding capacity and swelliong power are strong.

Description

The preparation method and sugarcane dietary fiber of a kind of sugarcane dietary fiber
Technical field
The invention belongs to dietary fiber preparing technical field, and in particular to the preparation method of a kind of sugarcane dietary fiber and sweet Sugarcane dietary fiber.
Background technology
Dietary fiber is a kind of important non-nutritive element, and it is a kind of SNSP and oligosaccharides in carbohydrate etc. Indigestion part, it can neither be digested and assimilated by human gastrointestinal tract, can not produce energy.But in recent years, more and more Research shows that the intake of dietary fiber is closely related with health, and constipation, obesity, glycosuria can be reduced by taking in enough dietary fibers The danger that disease, angiocardiopathy and intestinal tract occur.For example, dietary fiber largely can absorb moisture in enteron aisle, make excrement Soft condition is kept, effectively activates the beneficial bacteria in enteron aisle, promotes beneficial bacteria amount reproduction, creates enteron aisle environmental health, especially Apply to improve prolonged constipation;Meanwhile the dietary fiber content improved in meals can reduce the heat energy of intake, in enteron aisle Digesting and assimilating for interior nutrition also declines, and can also extend food residence time in the intestine, reduce the infiltration rate of glucose, not only The effect of with fat-reducing, and diabetic symptom can be improved.
Dietary fiber can be divided into soluble dietary fiber and non-soluble dietary fiber according to whether being dissolved in water.Many plants Most dietary fibers are non-soluble dietary fiber in raw material and its byproduct.Such as bagasse, it is cane sugar manufacture When squeezing after dregs, quality is thick and stiff, generally often produces one ton of sucrose, will produce about 1 ton of bagasse.Bagasse contains The fibre composition of higher amount, therefore used also as preparing dietary fiber.It is most that soluble dietary fiber is prepared in the food industry Number is to be modified non-soluble dietary fiber to change into soluble dietary fiber.However, the sugarcane of traditional processing method production There is the problems such as purity is low, yield poorly, poor taste in soluble dietary fiber, some even can also cause product moisture holding capacity mostly Reduce, influence dietary fiber effect.Therefore, a kind of preparation method for the sugarcane dietary fiber that can solve the problem that above mentioned problem of research and development is It is very necessary.
The content of the invention
The first object of the present invention is to provide a kind of preparation method of sugarcane dietary fiber.
The second object of the present invention is to provide what a kind of preparation method by described sugarcane dietary fiber was prepared Sugarcane dietary fiber.
The first object of the present invention, which is achieved in that, specifically includes following steps:
A, pre-treatment:Sugarcane is processed into remaining Sugarcane By-product after drying, crushing plus distilled water is in 110~120 DEG C of boilings Through concentrating, dry, crush after 30~40min, then raw material is adjusted feed moisture content using slowly sprinkling plus water while stirring To 100~150%, then stop stirring and taking out raw material, seal 5~30min, then raw material is fitted into steam explosion device, 1~ 4min rises to 0.8~1.0Mpa, moment release after 50~80s of pressurize, and it is 2.5~3 to take out raw material and add mass percent concentration NaOH solution, under 160~200W power extracting 25~30min obtains extract solution, then by extract solution with 5500~6000r/min Filtered after 3~4min of centrifugation, it is dietary fibre materials to take filter residue;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:3 ~5, add 1~4h of enzymatic hydrolysis, the mass ratio of dietary fibre materials and hydrolase is 1:0.02~0.03, hydrolysis temperature For 30~50 DEG C, pH value is 4.0~6.0, and it is semi-finished product that filtrate is taken after filtering;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:2~5, after mixing stand 1.5~2h, then with 4000~4500r/min centrifuges taking precipitate after 8~15min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods soluble dietary fiber.
The second object of the present invention is achieved in that what is be prepared by the preparation method of described sugarcane dietary fiber Sugarcane dietary fiber;This sugarcane dietary fiber has that purity is high, palatability is excellent, fiber diameter is small, moisture holding capacity and swelliong power The advantages of strong.
Beneficial effects of the present invention:The method of the present invention uses in preceding processing and carries out thermophilic digestion processing, vapour to raw material Quick-fried processing, the mode being combined is ultrasonically treated, can effectively destroy raw material mix tissue, its inclusion is fully exposed, and tie Removing impurities processing is closed, further reduces non-dietary fiber component in raw material, the raw material after pre-treatment not only contributes to reinforcing fiber The hydrolysis result of the complex enzyme of plain enzyme or cellulase and hemicellulase, makes enzymolysis more fully so as to improve manufactured goods purity, And the palatability of manufactured goods can be effectively improved;Purified again after enzymolysis, further improve purity, manufactured goods are soluble Dietary fiber has the advantages of purity is high, the preparation of the high-quality that is particularly suitable for use in soluble dietary fiber;The present invention is to pre-treatment Middle filtrate uses alkalinity extraction hydrophily hemicellulose, further improves soluble dietary fiber yield;The method system of the present invention Finished product soluble dietary fiber also has the advantages of fiber diameter is small, moisture holding capacity and swelliong power are strong;The method of the present invention By the use of Sugarcane By-product as raw material, the problem of wasting of resources and environmental pollution can be reduced.
Embodiment
With reference to embodiment, the present invention is further illustrated, but the present invention is not any limitation as in any way, Based on present invention teach that any conversion or replacement made, belong to protection scope of the present invention.
The preparation method of sugarcane dietary fiber of the present invention, specifically includes following steps:
A, pre-treatment:Sugarcane is processed into remaining Sugarcane By-product after drying, crushing plus distilled water is in 110~120 DEG C of boilings Through concentrating, dry, crush after 30~40min, then raw material is adjusted feed moisture content using slowly sprinkling plus water while stirring To 100~150%, then stop stirring and taking out raw material, seal 5~30min, then raw material is fitted into steam explosion device, 1~ 4min rises to 0.8~1.0Mpa, moment release after 50~80s of pressurize, and it is 2.5~3 to take out raw material and add mass percent concentration NaOH solution, under 160~200W power extracting 25~30min obtains extract solution, then by extract solution with 5500~6000r/min Filtered after 3~4min of centrifugation, it is dietary fibre materials to take filter residue;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:3 ~5, add 1~4h of enzymatic hydrolysis, the mass ratio of dietary fibre materials and hydrolase is 1:0.02~0.03, hydrolysis temperature For 30~50 DEG C, pH value is 4.0~6.0, and it is semi-finished product that filtrate is taken after filtering;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:2~5, after mixing stand 1.5~2h, then with 4000~4500r/min centrifuges taking precipitate after 8~15min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods soluble dietary fiber.
Filter residue adds the mass ratio 1 of water, filter residue and water in described step A:1~6, add removing impurities enzyme and digest 1~3.5h, The mass ratio of filter residue and removing impurities enzyme is 1:0.025~0.035, hydrolysis temperature is 25~55 DEG C, and pH value is 5.0~8.0, after filtering It is dietary fibre materials to take filter residue.
Described removing impurities enzyme is amylase and/or protease.
Described hydrolysis temperature is 50~55 DEG C, and pH value is 6~6.5.
Described hydrolase is cellulase or the complex enzyme of cellulase and hemicellulase.
Described Sugarcane By-product is bagasse.
Described removing impurities enzyme is amylase and protease, and the mass ratio of amylase and protease is 7~9:1~3.
Described hydrolase is the quality of the complex enzyme of cellulase and hemicellulase, cellulase and hemicellulase Than for 6~7:3~4.
Add acetic acid,diluted in the filtrate being filtrated to get in described step A, pH to 5 is adjusted, after stand at low temperature 4h separates out precipitation Filter residue is filtered to obtain, then adds the NaOH solution that mass percent concentration is 2 in filter residue and is well mixed, then adds acetic acid,diluted, regulation PH to 5, stand at low temperature 4h filter to obtain filter residue after separating out precipitation, and filter residue mixes after drying, crushing with step D manufactured goods.
Described drying is the one or more in expansion drying, vacuum drying, spray drying.
Sealing is sealing 20min in described step A.
The sugarcane dietary fiber that the preparation method of described sugarcane dietary fiber is prepared.
Embodiment 1
The preparation method of sugarcane dietary fiber, comprises the following steps:
A, pre-treatment:By bagasse through drying, crush after plus distilled water after 110 DEG C of boiling 30min through concentrating, drying, crushing, Again to raw material using slowly sprinkling adds water while stirring, feed moisture content is adjusted to 100%, then stops stirring and taking out original Material, 5min is sealed, then raw material is fitted into steam explosion device, risen to 0.8Mpa in 1min, moment release after pressurize 50s, take out original Material adds the NaOH solution that mass percent concentration is 2.5, and 25min is extracted under 160W power and obtains extract solution, then by extract solution Filtered after centrifuging 3min with 5500r/min, it is dietary fibre materials to take filter residue;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:3, Add enzymatic hydrolysis 1h, the mass ratio of dietary fibre materials and hydrolase is 1:0.02, hydrolysis temperature is 30 DEG C, and pH value is 4.0, it is semi-finished product that filtrate is taken after filtering;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:2,1.5h is stood after mixing, then with 4000r/ Taking precipitate after min centrifugations 8min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods soluble dietary fiber.
Embodiment 2
The preparation method of sugarcane dietary fiber, comprises the following steps:
A, pre-treatment:By bagasse through drying, crush after plus distilled water after 120 DEG C of boiling 40min through concentrating, drying, crushing, Again to raw material using slowly sprinkling adds water while stirring, feed moisture content is adjusted to 150%, then stops stirring and taking out original Material, 30min is sealed, then raw material is fitted into steam explosion device, risen to 1.0Mpa in 4min, moment release after pressurize 80s, take out original Material adds the NaOH solution that mass percent concentration is 3, and 30min is extracted under 200W power and obtains extract solution, then by extract solution with Filtered after 6000r/min centrifugations 4min, it is dietary fibre materials to take filter residue;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:5, Add enzymatic hydrolysis 4h, the mass ratio of dietary fibre materials and hydrolase is 1:0.03, hydrolysis temperature is 50 DEG C, and pH value is 6.0, it is semi-finished product that filtrate is taken after filtering;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:5,2h is stood after mixing, then with 4500r/min Taking precipitate after centrifugation 15min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods soluble dietary fiber.
Embodiment 3
The preparation method of sugarcane dietary fiber, comprises the following steps:
A, pre-treatment:By bagasse through drying, crush after plus distilled water after 115 DEG C of boiling 35min through concentrating, drying, crushing, Again to raw material using slowly sprinkling adds water while stirring, feed moisture content is adjusted to 125%, then stops stirring and taking out original Material, 17min is sealed, then raw material is fitted into steam explosion device, 0.9Mpa is risen in 2.5min, moment release after pressurize 65s, taking-up Raw material adds the NaOH solution that mass percent concentration is 2.7, and 27min is extracted under 180W power and obtains extract solution, then will extraction Liquid filters after centrifuging 3.5min with 5750r/min, and it is dietary fibre materials to take filter residue;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:4, Add enzymatic hydrolysis 2.5h, the mass ratio of dietary fibre materials and hydrolase is 1:0.025, hydrolysis temperature is 40 DEG C, pH It is worth for 5.0, it is semi-finished product that filtrate is taken after filtering;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:3.5,1.7h is stood after mixing, then with Taking precipitate after 4250r/min centrifugations 11min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods soluble dietary fiber.
Embodiment 4
The preparation method of sugarcane dietary fiber, comprises the following steps:
A, pre-treatment:By bagasse through drying, crush after plus distilled water after 110 DEG C of boiling 30min through concentrating, drying, crushing, Again to raw material using slowly sprinkling adds water while stirring, feed moisture content is adjusted to 100%, then stops stirring and taking out original Material, 5min is sealed, then raw material is fitted into steam explosion device, risen to 0.8Mpa in 1min, moment release after pressurize 50s, take out original Material adds the NaOH solution that mass percent concentration is 2.5, and 25min is extracted under 160W power and obtains extract solution, then by extract solution Filtered after centrifuging 3min with 5500r/min, take filter residue, filter residue adds the mass ratio 1 of water, filter residue and water:1, add removing impurities enzyme enzyme The mass ratio of solution 1h, filter residue and removing impurities enzyme is 1:0.025, hydrolysis temperature is 25 DEG C, and pH value 5.0, it is meals that filter residue is taken after filtering Eat fibrous raw material;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:3, Add enzymatic hydrolysis 1h, the mass ratio of dietary fibre materials and hydrolase is 1:0.02, hydrolysis temperature is 30 DEG C, and pH value is 4.0, it is semi-finished product that filtrate is taken after filtering;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:2,1.5h is stood after mixing, then with 4000r/ Taking precipitate after min centrifugations 8min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods soluble dietary fiber.
Embodiment 5
The preparation method of sugarcane dietary fiber, comprises the following steps:
A, pre-treatment:By bagasse through drying, crush after plus distilled water after 120 DEG C of boiling 40min through concentrating, drying, crushing, Again to raw material using slowly sprinkling adds water while stirring, feed moisture content is adjusted to 150%, then stops stirring and taking out original Material, 30min is sealed, then raw material is fitted into steam explosion device, risen to 1.0Mpa in 4min, moment release after pressurize 80s, take out original Material adds the NaOH solution that mass percent concentration is 3, and 30min is extracted under 200W power and obtains extract solution, then by extract solution with Filtered after 6000r/min centrifugations 4min, take filter residue, filter residue adds the mass ratio 1 of water, filter residue and water:6, add removing impurities enzyme enzymolysis The mass ratio of 3.5h, filter residue and removing impurities enzyme is 1:0.035, hydrolysis temperature is 55 DEG C, and pH value 8.0, it is meals that filter residue is taken after filtering Eat fibrous raw material;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:5, Add enzymatic hydrolysis 4h, the mass ratio of dietary fibre materials and hydrolase is 1:0.03, hydrolysis temperature is 50 DEG C, and pH value is 6.0, it is semi-finished product that filtrate is taken after filtering;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:5,2h is stood after mixing, then with 4500r/min Taking precipitate after centrifugation 15min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods soluble dietary fiber.
Embodiment 6
The preparation method of sugarcane dietary fiber, comprises the following steps:
A, pre-treatment:By bagasse through drying, crush after plus distilled water after 115 DEG C of boiling 35min through concentrating, drying, crushing, Again to raw material using slowly sprinkling adds water while stirring, feed moisture content is adjusted to 125%, then stops stirring and taking out original Material, 17min is sealed, then raw material is fitted into steam explosion device, 0.9Mpa is risen in 2.5min, moment release after pressurize 65s, taking-up Raw material adds the NaOH solution that mass percent concentration is 2.7, and 27min is extracted under 180W power and obtains extract solution, then will extraction Liquid filters after centrifuging 3.5min with 5750r/min, takes filter residue, filter residue adds the mass ratio 1 of water, filter residue and water:3.5, add de- The mass ratio of miscellaneous enzyme enzymolysis 2.2h, filter residue and removing impurities enzyme is 1:0.03, hydrolysis temperature is 40 DEG C, pH value 6.5, filter is taken after filtering Slag is dietary fibre materials;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:4, Add enzymatic hydrolysis 2.5h, the mass ratio of dietary fibre materials and hydrolase is 1:0.025, hydrolysis temperature is 40 DEG C, pH It is worth for 5.0, it is semi-finished product that filtrate is taken after filtering;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:3.5,1.7h is stood after mixing, then with Taking precipitate after 4250r/min centrifugations 11min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods soluble dietary fiber.
Embodiment 7
The preparation method of sugarcane dietary fiber, comprises the following steps:
A, pre-treatment:By bagasse through drying, crush after plus distilled water after 110 DEG C of boiling 30min through concentrating, drying, crushing, Again to raw material using slowly sprinkling adds water while stirring, feed moisture content is adjusted to 100%, then stops stirring and taking out original Material, 5min is sealed, then raw material is fitted into steam explosion device, risen to 0.8Mpa in 1min, moment release after pressurize 50s, take out original Material adds the NaOH solution that mass percent concentration is 2.5, and 25min is extracted under 160W power and obtains extract solution, then by extract solution Filtered after centrifuging 3min with 5500r/min, take filter residue, filter residue adds the mass ratio 1 of water, filter residue and water:1, add amylase enzyme The mass ratio of solution 1h, filter residue and amylase is 1:0.025, hydrolysis temperature is 25 DEG C, and pH value 5.0, it is meals that filter residue is taken after filtering Eat fibrous raw material;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:3, Add enzymatic hydrolysis 1h, the mass ratio of dietary fibre materials and hydrolase is 1:0.02, hydrolysis temperature is 30 DEG C, and pH value is 4.0, it is semi-finished product that filtrate is taken after filtering;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:2,1.5h is stood after mixing, then with 4000r/ Taking precipitate after min centrifugations 8min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods soluble dietary fiber.
Embodiment 8
The preparation method of sugarcane dietary fiber, comprises the following steps:
A, pre-treatment:By bagasse through drying, crush after plus distilled water after 120 DEG C of boiling 40min through concentrating, drying, crushing, Again to raw material using slowly sprinkling adds water while stirring, feed moisture content is adjusted to 150%, then stops stirring and taking out original Material, 30min is sealed, then raw material is fitted into steam explosion device, risen to 1.0Mpa in 4min, moment release after pressurize 80s, take out original Material adds the NaOH solution that mass percent concentration is 3, and 30min is extracted under 200W power and obtains extract solution, then by extract solution with Filtered after 6000r/min centrifugations 4min, take filter residue, filter residue adds the mass ratio 1 of water, filter residue and water:6, add amylase enzymolysis The mass ratio of 3.5h, filter residue and amylase is 1:0.035, hydrolysis temperature is 55 DEG C, and pH value 8.0, it is meals that filter residue is taken after filtering Eat fibrous raw material;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:5, Add enzymatic hydrolysis 4h, the mass ratio of dietary fibre materials and hydrolase is 1:0.03, hydrolysis temperature is 50 DEG C, and pH value is 6.0, it is semi-finished product that filtrate is taken after filtering;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:5,2h is stood after mixing, then with 4500r/min Taking precipitate after centrifugation 15min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods soluble dietary fiber.
Embodiment 9
The preparation method of sugarcane dietary fiber, comprises the following steps:
A, pre-treatment:By bagasse through drying, crush after plus distilled water after 115 DEG C of boiling 35min through concentrating, drying, crushing, Again to raw material using slowly sprinkling adds water while stirring, feed moisture content is adjusted to 125%, then stops stirring and taking out original Material, 17min is sealed, then raw material is fitted into steam explosion device, 0.9Mpa is risen in 2.5min, moment release after pressurize 65s, taking-up Raw material adds the NaOH solution that mass percent concentration is 2.7, and 27min is extracted under 180W power and obtains extract solution, then will extraction Liquid filters after centrifuging 3.5min with 5750r/min, takes filter residue, filter residue adds the mass ratio 1 of water, filter residue and water:3.5, add shallow lake The mass ratio of powder enzyme enzymolysis 2.2h, filter residue and amylase is 1:0.03, hydrolysis temperature is 40 DEG C, pH value 6.5, filter is taken after filtering Slag is dietary fibre materials;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:4, Add enzymatic hydrolysis 2.5h, the mass ratio of dietary fibre materials and hydrolase is 1:0.025, hydrolysis temperature is 40 DEG C, pH It is worth for 5.0, it is semi-finished product that filtrate is taken after filtering;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:3.5,1.7h is stood after mixing, then with Taking precipitate after 4250r/min centrifugations 11min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods soluble dietary fiber.
Embodiment 10
The preparation method of sugarcane dietary fiber, comprises the following steps:
A, pre-treatment:By bagasse through drying, crush after plus distilled water after 110 DEG C of boiling 30min through concentrating, drying, crushing, Again to raw material using slowly sprinkling adds water while stirring, feed moisture content is adjusted to 100%, then stops stirring and taking out original Material, 5min is sealed, then raw material is fitted into steam explosion device, risen to 0.8Mpa in 1min, moment release after pressurize 50s, take out original Material adds the NaOH solution that mass percent concentration is 2.5, and 25min is extracted under 160W power and obtains extract solution, then by extract solution Filtered after centrifuging 3min with 5500r/min, take filter residue, filter residue adds the mass ratio 1 of water, filter residue and water:1, add protease enzyme The mass ratio of solution 1h, filter residue and protease is 1:0.025, hydrolysis temperature is 25 DEG C, and pH value 5.0, it is meals that filter residue is taken after filtering Eat fibrous raw material;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:3, Add enzymatic hydrolysis 1h, the mass ratio of dietary fibre materials and hydrolase is 1:0.02, hydrolysis temperature is 30 DEG C, and pH value is 4.0, it is semi-finished product that filtrate is taken after filtering;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:2,1.5h is stood after mixing, then with 4000r/ Taking precipitate after min centrifugations 8min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods soluble dietary fiber.
Embodiment 11
The preparation method of sugarcane dietary fiber, comprises the following steps:
A, pre-treatment:By bagasse through drying, crush after plus distilled water after 120 DEG C of boiling 40min through concentrating, drying, crushing, Again to raw material using slowly sprinkling adds water while stirring, feed moisture content is adjusted to 150%, then stops stirring and taking out original Material, 30min is sealed, then raw material is fitted into steam explosion device, risen to 1.0Mpa in 4min, moment release after pressurize 80s, take out original Material adds the NaOH solution that mass percent concentration is 3, and 30min is extracted under 200W power and obtains extract solution, then by extract solution with Filtered after 6000r/min centrifugations 4min, take filter residue, filter residue adds the mass ratio 1 of water, filter residue and water:6, add protease hydrolyzed The mass ratio of 3.5h, filter residue and protease is 1:0.035, hydrolysis temperature is 55 DEG C, and pH value 8.0, it is meals that filter residue is taken after filtering Eat fibrous raw material;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:5, Add enzymatic hydrolysis 4h, the mass ratio of dietary fibre materials and hydrolase is 1:0.03, hydrolysis temperature is 50 DEG C, and pH value is 6.0, it is semi-finished product that filtrate is taken after filtering;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:5,2h is stood after mixing, then with 4500r/min Taking precipitate after centrifugation 15min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods soluble dietary fiber.
Embodiment 12
The preparation method of sugarcane dietary fiber, comprises the following steps:
A, pre-treatment:By bagasse through drying, crush after plus distilled water after 115 DEG C of boiling 35min through concentrating, drying, crushing, Again to raw material using slowly sprinkling adds water while stirring, feed moisture content is adjusted to 125%, then stops stirring and taking out original Material, 17min is sealed, then raw material is fitted into steam explosion device, 0.9Mpa is risen in 2.5min, moment release after pressurize 65s, taking-up Raw material adds the NaOH solution that mass percent concentration is 2.7, and 27min is extracted under 180W power and obtains extract solution, then will extraction Liquid filters after centrifuging 3.5min with 5750r/min, takes filter residue, filter residue adds the mass ratio 1 of water, filter residue and water:3.5, add egg The mass ratio of white enzyme enzymolysis 2.2h, filter residue and protease is 1:0.03, hydrolysis temperature is 40 DEG C, pH value 6.5, filter is taken after filtering Slag is dietary fibre materials;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:4, Add enzymatic hydrolysis 2.5h, the mass ratio of dietary fibre materials and hydrolase is 1:0.025, hydrolysis temperature is 40 DEG C, pH It is worth for 5.0, it is semi-finished product that filtrate is taken after filtering;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:3.5,1.7h is stood after mixing, then with Taking precipitate after 4250r/min centrifugations 11min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods soluble dietary fiber.
Embodiment 13
The preparation method of sugarcane dietary fiber, comprises the following steps:
A, pre-treatment:By bagasse through drying, crush after plus distilled water after 110 DEG C of boiling 30min through concentrating, drying, crushing, Again to raw material using slowly sprinkling adds water while stirring, feed moisture content is adjusted to 100%, then stops stirring and taking out original Material, 5min is sealed, then raw material is fitted into steam explosion device, risen to 0.8Mpa in 1min, moment release after pressurize 50s, take out original Material adds the NaOH solution that mass percent concentration is 2.5, and 25min is extracted under 160W power and obtains extract solution, then by extract solution Filtered after centrifuging 3min with 5500r/min, take filter residue, filter residue adds the mass ratio 1 of water, filter residue and water:1, add removing impurities enzyme enzyme 1h is solved, described removing impurities enzyme be amylase and protease, and the mass ratio of amylase and protease is 7:3, filter residue and removing impurities enzyme Mass ratio is 1:0.025, hydrolysis temperature is 25 DEG C, and pH value 5.0, it is dietary fibre materials that filter residue is taken after filtering;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:3, Add enzymatic hydrolysis 1h, the mass ratio of dietary fibre materials and hydrolase is 1:0.02, hydrolysis temperature is 30 DEG C, and pH value is 4.0, it is semi-finished product that filtrate is taken after filtering;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:2,1.5h is stood after mixing, then with 4000r/ Taking precipitate after min centrifugations 8min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods soluble dietary fiber.
Embodiment 14
The preparation method of sugarcane dietary fiber, comprises the following steps:
A, pre-treatment:By bagasse through drying, crush after plus distilled water after 120 DEG C of boiling 40min through concentrating, drying, crushing, Again to raw material using slowly sprinkling adds water while stirring, feed moisture content is adjusted to 150%, then stops stirring and taking out original Material, 30min is sealed, then raw material is fitted into steam explosion device, risen to 1.0Mpa in 4min, moment release after pressurize 80s, take out original Material adds the NaOH solution that mass percent concentration is 3, and 30min is extracted under 200W power and obtains extract solution, then by extract solution with Filtered after 6000r/min centrifugations 4min, take filter residue, filter residue adds the mass ratio 1 of water, filter residue and water:6, add removing impurities enzyme enzymolysis 3.5h, described removing impurities enzyme are amylase and protease, and the mass ratio of amylase and protease is 9:1, filter residue and removing impurities enzyme Mass ratio is 1:0.035, hydrolysis temperature is 55 DEG C, and pH value 8.0, it is dietary fibre materials that filter residue is taken after filtering;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:5, Add enzymatic hydrolysis 4h, the mass ratio of dietary fibre materials and hydrolase is 1:0.03, hydrolysis temperature is 50 DEG C, and pH value is 6.0, it is semi-finished product that filtrate is taken after filtering;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:5,2h is stood after mixing, then with 4500r/min Taking precipitate after centrifugation 15min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods soluble dietary fiber.
Embodiment 15
The preparation method of sugarcane dietary fiber, comprises the following steps:
A, pre-treatment:By bagasse through drying, crush after plus distilled water after 115 DEG C of boiling 35min through concentrating, drying, crushing, Again to raw material using slowly sprinkling adds water while stirring, feed moisture content is adjusted to 125%, then stops stirring and taking out original Material, 17min is sealed, then raw material is fitted into steam explosion device, 0.9Mpa is risen in 2.5min, moment release after pressurize 65s, taking-up Raw material adds the NaOH solution that mass percent concentration is 2.7, and 27min is extracted under 180W power and obtains extract solution, then will extraction Liquid filters after centrifuging 3.5min with 5750r/min, takes filter residue, filter residue adds the mass ratio 1 of water, filter residue and water:3.5, add de- Miscellaneous enzyme digests 2.2h, and described removing impurities enzyme be amylase and protease, and the mass ratio of amylase and protease is 8:2, filter residue with The mass ratio of removing impurities enzyme is 1:0.03, hydrolysis temperature is 40 DEG C, and pH value 6.5, it is dietary fibre materials that filter residue is taken after filtering;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:4, Add enzymatic hydrolysis 2.5h, the mass ratio of dietary fibre materials and hydrolase is 1:0.025, hydrolysis temperature is 40 DEG C, pH It is worth for 5.0, it is semi-finished product that filtrate is taken after filtering;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:3.5,1.7h is stood after mixing, then with Taking precipitate after 4250r/min centrifugations 11min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods soluble dietary fiber.
Embodiment 16
The preparation method of sugarcane dietary fiber, comprises the following steps:
A, pre-treatment:By bagasse through drying, crush after plus distilled water after 110 DEG C of boiling 30min through concentrating, drying, crushing, Again to raw material using slowly sprinkling adds water while stirring, feed moisture content is adjusted to 100%, then stops stirring and taking out original Material, 5min is sealed, then raw material is fitted into steam explosion device, risen to 0.8Mpa in 1min, moment release after pressurize 50s, take out original Material adds the NaOH solution that mass percent concentration is 2.5, and 25min is extracted under 160W power and obtains extract solution, then by extract solution Filtered after centrifuging 3min with 5500r/min, take filter residue, filter residue adds the mass ratio 1 of water, filter residue and water:1, add removing impurities enzyme enzyme 1h is solved, described removing impurities enzyme be amylase and protease, and the mass ratio of amylase and protease is 7:3, filter residue and removing impurities enzyme Mass ratio is 1:0.025, hydrolysis temperature is 25 DEG C, and pH value 5.0, it is dietary fibre materials that filter residue is taken after filtering;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:3, Add cellulase degradation 1h, the mass ratio of dietary fibre materials and cellulase is 1:0.02, hydrolysis temperature is 30 DEG C, pH It is worth for 4.0, it is semi-finished product that filtrate is taken after filtering;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:2,1.5h is stood after mixing, then with 4000r/ Taking precipitate after min centrifugations 8min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods soluble dietary fiber.
Embodiment 17
The preparation method of sugarcane dietary fiber, comprises the following steps:
A, pre-treatment:By bagasse through drying, crush after plus distilled water after 120 DEG C of boiling 40min through concentrating, drying, crushing, Again to raw material using slowly sprinkling adds water while stirring, feed moisture content is adjusted to 150%, then stops stirring and taking out original Material, 30min is sealed, then raw material is fitted into steam explosion device, risen to 1.0Mpa in 4min, moment release after pressurize 80s, take out original Material adds the NaOH solution that mass percent concentration is 3, and 30min is extracted under 200W power and obtains extract solution, then by extract solution with Filtered after 6000r/min centrifugations 4min, take filter residue, filter residue adds the mass ratio 1 of water, filter residue and water:6, add removing impurities enzyme enzymolysis 3.5h, described removing impurities enzyme are amylase and protease, and the mass ratio of amylase and protease is 9:1, filter residue and removing impurities enzyme Mass ratio is 1:0.035, hydrolysis temperature is 55 DEG C, and pH value 8.0, it is dietary fibre materials that filter residue is taken after filtering;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:5, Add cellulase degradation 4h, the mass ratio of dietary fibre materials and cellulase is 1:0.03, hydrolysis temperature is 50 DEG C, pH It is worth for 6.0, it is semi-finished product that filtrate is taken after filtering;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:5,2h is stood after mixing, then with 4500r/min Taking precipitate after centrifugation 15min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods soluble dietary fiber.
Embodiment 18
The preparation method of sugarcane dietary fiber, comprises the following steps:
A, pre-treatment:By bagasse through drying, crush after plus distilled water after 115 DEG C of boiling 35min through concentrating, drying, crushing, Again to raw material using slowly sprinkling adds water while stirring, feed moisture content is adjusted to 125%, then stops stirring and taking out original Material, 17min is sealed, then raw material is fitted into steam explosion device, 0.9Mpa is risen in 2.5min, moment release after pressurize 65s, taking-up Raw material adds the NaOH solution that mass percent concentration is 2.7, and 27min is extracted under 180W power and obtains extract solution, then will extraction Liquid filters after centrifuging 3.5min with 5750r/min, takes filter residue, filter residue adds the mass ratio 1 of water, filter residue and water:3.5, add de- Miscellaneous enzyme digests 2.2h, and described removing impurities enzyme be amylase and protease, and the mass ratio of amylase and protease is 8:2, filter residue with The mass ratio of removing impurities enzyme is 1:0.03, hydrolysis temperature is 40 DEG C, and pH value 6.5, it is dietary fibre materials that filter residue is taken after filtering;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:4, Add cellulase degradation 2.5h, the mass ratio of dietary fibre materials and cellulase is 1:0.025, hydrolysis temperature 40 DEG C, pH value 5.0, it is semi-finished product that filtrate is taken after filtering;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:3.5,1.7h is stood after mixing, then with Taking precipitate after 4250r/min centrifugations 11min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods soluble dietary fiber.
Embodiment 19
The preparation method of sugarcane dietary fiber, comprises the following steps:
A, pre-treatment:By bagasse through drying, crush after plus distilled water after 110 DEG C of boiling 30min through concentrating, drying, crushing, Again to raw material using slowly sprinkling adds water while stirring, feed moisture content is adjusted to 100%, then stops stirring and taking out original Material, 5min is sealed, then raw material is fitted into steam explosion device, risen to 0.8Mpa in 1min, moment release after pressurize 50s, take out original Material adds the NaOH solution that mass percent concentration is 2.5, and 25min is extracted under 160W power and obtains extract solution, then by extract solution Filtered after centrifuging 3min with 5500r/min, take filter residue, filter residue adds the mass ratio 1 of water, filter residue and water:1, add removing impurities enzyme enzyme 1h is solved, described removing impurities enzyme be amylase and protease, and the mass ratio of amylase and protease is 7:3, filter residue and removing impurities enzyme Mass ratio is 1:0.025, hydrolysis temperature is 25 DEG C, and pH value 5.0, it is dietary fibre materials that filter residue is taken after filtering;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:3, Add enzymatic hydrolysis 1h, described hydrolase is cellulase and hemicellulase, cellulase and hemicellulase Mass ratio is 6:4, the mass ratio of dietary fibre materials and hydrolase is 1:0.02, hydrolysis temperature is 30 DEG C, pH value 4.0, mistake It is semi-finished product that filtrate is taken after filter;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:2,1.5h is stood after mixing, then with 4000r/ Taking precipitate after min centrifugations 8min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods soluble dietary fiber.
Embodiment 20
The preparation method of sugarcane dietary fiber, comprises the following steps:
A, pre-treatment:By bagasse through drying, crush after plus distilled water after 120 DEG C of boiling 40min through concentrating, drying, crushing, Again to raw material using slowly sprinkling adds water while stirring, feed moisture content is adjusted to 150%, then stops stirring and taking out original Material, 30min is sealed, then raw material is fitted into steam explosion device, risen to 1.0Mpa in 4min, moment release after pressurize 80s, take out original Material adds the NaOH solution that mass percent concentration is 3, and 30min is extracted under 200W power and obtains extract solution, then by extract solution with Filtered after 6000r/min centrifugations 4min, take filter residue, filter residue adds the mass ratio 1 of water, filter residue and water:6, add removing impurities enzyme enzymolysis 3.5h, described removing impurities enzyme are amylase and protease, and the mass ratio of amylase and protease is 9:1, filter residue and removing impurities enzyme Mass ratio is 1:0.035, hydrolysis temperature is 55 DEG C, and pH value 8.0, it is dietary fibre materials that filter residue is taken after filtering;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:5, Add enzymatic hydrolysis 4h, described hydrolase is cellulase and hemicellulase, cellulase and hemicellulase Mass ratio is 7:3, the mass ratio of dietary fibre materials and hydrolase is 1:0.03, hydrolysis temperature is 50 DEG C, pH value 6.0, mistake It is semi-finished product that filtrate is taken after filter;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:5,2h is stood after mixing, then with 4500r/min Taking precipitate after centrifugation 15min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods soluble dietary fiber.
Embodiment 21
The preparation method of sugarcane dietary fiber, comprises the following steps:
A, pre-treatment:By bagasse through drying, crush after plus distilled water after 115 DEG C of boiling 35min through concentrating, drying, crushing, Again to raw material using slowly sprinkling adds water while stirring, feed moisture content is adjusted to 125%, then stops stirring and taking out original Material, 17min is sealed, then raw material is fitted into steam explosion device, 0.9Mpa is risen in 2.5min, moment release after pressurize 65s, taking-up Raw material adds the NaOH solution that mass percent concentration is 2.7, and 27min is extracted under 180W power and obtains extract solution, then will extraction Liquid filters after centrifuging 3.5min with 5750r/min, takes filter residue, filter residue adds the mass ratio 1 of water, filter residue and water:3.5, add de- Miscellaneous enzyme digests 2.2h, and described removing impurities enzyme be amylase and protease, and the mass ratio of amylase and protease is 8:2, filter residue with The mass ratio of removing impurities enzyme is 1:0.03, hydrolysis temperature is 40 DEG C, and pH value 6.5, it is dietary fibre materials that filter residue is taken after filtering;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:4, Add enzymatic hydrolysis 2.5h, described hydrolase is cellulase and hemicellulase, cellulase and hemicellulase Mass ratio be 6.5:3.5, the mass ratio of dietary fibre materials and hydrolase is 1:0.025, hydrolysis temperature is 40 DEG C, and pH value is 5.0, it is semi-finished product that filtrate is taken after filtering;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:3.5,1.7h is stood after mixing, then with Taking precipitate after 4250r/min centrifugations 11min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods soluble dietary fiber.
Embodiment 22
The preparation method of sugarcane dietary fiber, comprises the following steps:
A, pre-treatment:By bagasse through drying, crush after plus distilled water after 110 DEG C of boiling 30min through concentrating, drying, crushing, Again to raw material using slowly sprinkling adds water while stirring, feed moisture content is adjusted to 100%, then stops stirring and taking out original Material, 5min is sealed, then raw material is fitted into steam explosion device, risen to 0.8Mpa in 1min, moment release after pressurize 50s, take out original Material adds the NaOH solution that mass percent concentration is 2.5, and 25min is extracted under 160W power and obtains extract solution, then by extract solution Filtered after centrifuging 3min with 5500r/min, take filter residue, filter residue adds the mass ratio 1 of water, filter residue and water:1, add removing impurities enzyme enzyme 1h is solved, described removing impurities enzyme be amylase and protease, and the mass ratio of amylase and protease is 7:3, filter residue and removing impurities enzyme Mass ratio is 1:0.025, hydrolysis temperature is 50 DEG C, and pH value 6.0, it is dietary fibre materials that filter residue is taken after filtering;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:3, Add enzymatic hydrolysis 1h, described hydrolase is cellulase and hemicellulase, cellulase and hemicellulase Mass ratio is 6:4, the mass ratio of dietary fibre materials and hydrolase is 1:0.02, hydrolysis temperature is 30 DEG C, pH value 4.0, mistake It is semi-finished product that filtrate is taken after filter;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:2,1.5h is stood after mixing, then with 4000r/ Taking precipitate after min centrifugations 8min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods soluble dietary fiber.
Embodiment 23
The preparation method of sugarcane dietary fiber, comprises the following steps:
A, pre-treatment:By bagasse through drying, crush after plus distilled water after 120 DEG C of boiling 40min through concentrating, drying, crushing, Again to raw material using slowly sprinkling adds water while stirring, feed moisture content is adjusted to 150%, then stops stirring and taking out original Material, 30min is sealed, then raw material is fitted into steam explosion device, risen to 1.0Mpa in 4min, moment release after pressurize 80s, take out original Material adds the NaOH solution that mass percent concentration is 3, and 30min is extracted under 200W power and obtains extract solution, then by extract solution with Filtered after 6000r/min centrifugations 4min, take filter residue, filter residue adds the mass ratio 1 of water, filter residue and water:6, add removing impurities enzyme enzymolysis 3.5h, described removing impurities enzyme are amylase and protease, and the mass ratio of amylase and protease is 9:1, filter residue and removing impurities enzyme Mass ratio is 1:0.035, hydrolysis temperature is 55 DEG C, and pH value 6.5, it is dietary fibre materials that filter residue is taken after filtering;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:5, Add enzymatic hydrolysis 4h, described hydrolase is cellulase and hemicellulase, cellulase and hemicellulase Mass ratio is 7:3, the mass ratio of dietary fibre materials and hydrolase is 1:0.03, hydrolysis temperature is 50 DEG C, pH value 6.0, mistake It is semi-finished product that filtrate is taken after filter;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:5,2h is stood after mixing, then with 4500r/min Taking precipitate after centrifugation 15min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods soluble dietary fiber.
Embodiment 24
The preparation method of sugarcane dietary fiber, comprises the following steps:
A, pre-treatment:By bagasse through drying, crush after plus distilled water after 110 DEG C of boiling 30min through concentrating, drying, crushing, Again to raw material using slowly sprinkling adds water while stirring, feed moisture content is adjusted to 100%, then stops stirring and taking out original Material, 5min is sealed, then raw material is fitted into steam explosion device, risen to 0.8Mpa in 1min, moment release after pressurize 50s, take out original Material adds the NaOH solution that mass percent concentration is 2.5, and 25min is extracted under 160W power and obtains extract solution, then by extract solution Filtered after centrifuging 3min with 5500r/min, take filter residue, filter residue adds the mass ratio 1 of water, filter residue and water:1, add removing impurities enzyme enzyme 1h is solved, described removing impurities enzyme be amylase and protease, and the mass ratio of amylase and protease is 7:3, filter residue and removing impurities enzyme Mass ratio is 1:0.025, hydrolysis temperature is 50 DEG C, pH value 6.0, and it is dietary fibre materials that filter residue is taken after filtering, is added in filtrate Acetic acid,diluted, pH to 5 is adjusted, stand at low temperature 4h filters to obtain filter residue after separating out precipitation, then mass percent concentration is added in filter residue It is well mixed for 2 NaOH solution, then adds acetic acid,diluted, adjust pH to 5, stand at low temperature 4h filters to obtain filter residue, filter after separating out precipitation Slag obtains manufactured goods A after drying, crushing;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:3, Add enzymatic hydrolysis 1h, described hydrolase is cellulase and hemicellulase, cellulase and hemicellulase Mass ratio is 6:4, the mass ratio of dietary fibre materials and hydrolase is 1:0.02, hydrolysis temperature is 30 DEG C, pH value 4.0, mistake It is semi-finished product that filtrate is taken after filter;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:2,1.5h is stood after mixing, then with 4000r/ Taking precipitate after min centrifugations 8min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods B, manufactured goods A and manufactured goods B are mixed Close to obtain soluble dietary fiber.
Embodiment 25
The preparation method of sugarcane dietary fiber, comprises the following steps:
A, pre-treatment:By bagasse through drying, crush after plus distilled water after 120 DEG C of boiling 40min through concentrating, drying, crushing, Again to raw material using slowly sprinkling adds water while stirring, feed moisture content is adjusted to 150%, then stops stirring and taking out original Material, 30min is sealed, then raw material is fitted into steam explosion device, risen to 1.0Mpa in 4min, moment release after pressurize 80s, take out original Material adds the NaOH solution that mass percent concentration is 3, and 30min is extracted under 200W power and obtains extract solution, then by extract solution with Filtered after 6000r/min centrifugations 4min, take filter residue, filter residue adds the mass ratio 1 of water, filter residue and water:6, add removing impurities enzyme enzymolysis 3.5h, described removing impurities enzyme are amylase and protease, and the mass ratio of amylase and protease is 9:1, filter residue and removing impurities enzyme Mass ratio is 1:0.035, hydrolysis temperature is 55 DEG C, pH value 6.5, and it is dietary fibre materials that filter residue is taken after filtering, is added in filtrate Acetic acid,diluted, pH to 5 is adjusted, stand at low temperature 4h filters to obtain filter residue after separating out precipitation, then mass percent concentration is added in filter residue It is well mixed for 2 NaOH solution, then adds acetic acid,diluted, adjust pH to 5, stand at low temperature 4h filters to obtain filter residue, filter after separating out precipitation Slag obtains manufactured goods A after drying, crushing;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:5, Add enzymatic hydrolysis 4h, described hydrolase is cellulase and hemicellulase, cellulase and hemicellulase Mass ratio is 7:3, the mass ratio of dietary fibre materials and hydrolase is 1:0.03, hydrolysis temperature is 50 DEG C, pH value 6.0, mistake It is semi-finished product that filtrate is taken after filter;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:5,2h is stood after mixing, then with 4500r/min Taking precipitate after centrifugation 15min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods B, manufactured goods A and manufactured goods B are mixed Close to obtain soluble dietary fiber.
Embodiment 26
The sugarcane dietary fiber being prepared by the preparation method of embodiment 3.
Embodiment 27
The sugarcane dietary fiber being prepared by the preparation method of embodiment 25.

Claims (10)

1. a kind of preparation method of sugarcane dietary fiber, it is characterised in that specifically include following steps:
A, pre-treatment:Sugarcane is processed into remaining Sugarcane By-product after drying, crushing plus distilled water is in 110~120 DEG C of boilings Through concentrating, dry, crush after 30~40min, then raw material is adjusted feed moisture content using slowly sprinkling plus water while stirring To 100~150%, then stop stirring and taking out raw material, seal 5~30min, then raw material is fitted into steam explosion device, 1~ 4min rises to 0.8~1.0Mpa, moment release after 50~80s of pressurize, and it is 2.5~3 to take out raw material and add mass percent concentration NaOH solution, under 160~200W power extracting 25~30min obtains extract solution, then by extract solution with 5500~6000r/min Filtered after 3~4min of centrifugation, it is dietary fibre materials to take filter residue;
B, prepared by semi-finished product:The obtained dietary fibre materials of step A are added into water, the mass ratio of dietary fibre materials and water is 1:3 ~5, add 1~4h of enzymatic hydrolysis, the mass ratio of dietary fibre materials and hydrolase is 1:0.02~0.03, hydrolysis temperature For 30~50 DEG C, pH value is 4.0~6.0, and it is semi-finished product that filtrate is taken after filtering;
C, purify:To the semi-finished product heat inactivation in step B, filtrate is taken after filtering, concentrating filter liquor obtains concentrate, then dense Add absolute ethyl alcohol in contracting liquid, the mass ratio of concentrate and absolute ethyl alcohol is 1:2~5, after mixing stand 1.5~2h, then with 4000~4500r/min centrifuges taking precipitate after 8~15min;
D, post-process:By obtained sediment in step C through homogeneous, be drying to obtain manufactured goods soluble dietary fiber.
2. the preparation method of sugarcane dietary fiber according to claim 1, it is characterised in that the filter residue described in step A adds The mass ratio 1 of water, filter residue and water:1~6, add removing impurities enzyme and digest 1~3.5h, the mass ratio of filter residue and removing impurities enzyme is 1: 0.025~0.035, hydrolysis temperature is 25~55 DEG C, and pH value is 5.0~8.0, and it is dietary fibre materials that filter residue is taken after filtering.
3. the preparation method of sugarcane dietary fiber according to claim 2, it is characterised in that described removing impurities enzyme is starch Enzyme and/or protease.
4. the preparation method of sugarcane dietary fiber according to claim 2, it is characterised in that described hydrolysis temperature is 50 ~55 DEG C, pH value is 6~6.5.
5. the preparation method of sugarcane dietary fiber according to claim 1, it is characterised in that described hydrolase is fiber The complex enzyme of plain enzyme or cellulase and hemicellulase.
6. the preparation method of sugarcane dietary fiber according to claim 1, it is characterised in that described Sugarcane By-product is Bagasse.
7. the preparation method of the sugarcane dietary fiber according to Claims 2 or 3, it is characterised in that described removing impurities enzyme is shallow lake The mass ratio of powder enzyme and protease, amylase and protease is 7~9:1~3.
8. the preparation method of sugarcane dietary fiber according to claim 1 or 5, it is characterised in that described hydrolase is fibre Tie up the complex enzyme of plain enzyme and hemicellulase, the mass ratio of cellulase and hemicellulase is 6~7:3~4.
9. the preparation method of sugarcane dietary fiber according to claim 1, it is characterised in that filtering described in step A To filtrate in plus acetic acid,diluted, adjust pH to 5, stand at low temperature 4h filters to obtain filter residue after separating out precipitation, then matter added in filter residue The NaOH solution that amount percent concentration is 2 is well mixed, then adds acetic acid,diluted, adjusts pH to 5, and stand at low temperature 4h separates out mistake after precipitation Filter residue is filtered to obtain, filter residue mixes after drying, crushing with step D manufactured goods.
10. the sugarcane meals that a kind of preparation method of sugarcane dietary fiber as described in claim 1~9 is any is prepared are fine Dimension.
CN201710664917.5A 2017-08-07 2017-08-07 The preparation method and sugarcane dietary fiber of a kind of sugarcane dietary fiber Pending CN107692243A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109602027A (en) * 2018-12-10 2019-04-12 武汉轻工大学 A kind of method that extrusion assistance enzymolysis extracts soluble dietary fiber in pueraria root residue
CN110652014A (en) * 2019-10-16 2020-01-07 梁乐 Method for preparing dietary fiber from zedoary turmeric leaves

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US5622738A (en) * 1989-05-16 1997-04-22 Nihon Shokuhin Kako Co., Ltd. Method of preparing water-soluble dietary fiber
CN102599352A (en) * 2012-04-09 2012-07-25 苏州昆蓝生物科技有限公司 Treating agent for non-fermentative straw feed and application method thereof
CN103859422A (en) * 2014-03-18 2014-06-18 中国热带农业科学院海口实验站 Production method of banana flower soluble dietary fiber
CN106539097A (en) * 2016-09-27 2017-03-29 济南米铎碳新能源科技有限公司 The water-soluble method with Water insoluble dietary fiber is produced simultaneously using bagasse
CN106912964A (en) * 2015-12-25 2017-07-04 山东龙力生物科技股份有限公司 Soluble dietary fiber and preparation method thereof

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Publication number Priority date Publication date Assignee Title
US5622738A (en) * 1989-05-16 1997-04-22 Nihon Shokuhin Kako Co., Ltd. Method of preparing water-soluble dietary fiber
CN102599352A (en) * 2012-04-09 2012-07-25 苏州昆蓝生物科技有限公司 Treating agent for non-fermentative straw feed and application method thereof
CN103859422A (en) * 2014-03-18 2014-06-18 中国热带农业科学院海口实验站 Production method of banana flower soluble dietary fiber
CN106912964A (en) * 2015-12-25 2017-07-04 山东龙力生物科技股份有限公司 Soluble dietary fiber and preparation method thereof
CN106539097A (en) * 2016-09-27 2017-03-29 济南米铎碳新能源科技有限公司 The water-soluble method with Water insoluble dietary fiber is produced simultaneously using bagasse

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109602027A (en) * 2018-12-10 2019-04-12 武汉轻工大学 A kind of method that extrusion assistance enzymolysis extracts soluble dietary fiber in pueraria root residue
CN110652014A (en) * 2019-10-16 2020-01-07 梁乐 Method for preparing dietary fiber from zedoary turmeric leaves

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