CN106509586A - Preparation method of preservative for biomass food - Google Patents
Preparation method of preservative for biomass food Download PDFInfo
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- CN106509586A CN106509586A CN201610945110.4A CN201610945110A CN106509586A CN 106509586 A CN106509586 A CN 106509586A CN 201610945110 A CN201610945110 A CN 201610945110A CN 106509586 A CN106509586 A CN 106509586A
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- Prior art keywords
- preservative
- micro
- charcoal
- standby
- protamine
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- 239000002028 Biomass Substances 0.000 title claims abstract description 11
- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- 235000013305 food Nutrition 0.000 title abstract description 19
- 239000003755 preservative agent Substances 0.000 title abstract description 19
- 230000002335 preservative effect Effects 0.000 title abstract description 18
- 239000005452 food preservative Substances 0.000 claims abstract description 21
- 235000019249 food preservative Nutrition 0.000 claims abstract description 21
- 102000007327 Protamines Human genes 0.000 claims abstract description 19
- 108010007568 Protamines Proteins 0.000 claims abstract description 19
- 229940048914 protamine Drugs 0.000 claims abstract description 19
- 239000003610 charcoal Substances 0.000 claims abstract description 18
- 238000000855 fermentation Methods 0.000 claims abstract description 14
- 230000004151 fermentation Effects 0.000 claims abstract description 14
- 241000241413 Propolis Species 0.000 claims abstract description 8
- 210000003608 fece Anatomy 0.000 claims abstract description 8
- 239000010871 livestock manure Substances 0.000 claims abstract description 8
- 229940069949 propolis Drugs 0.000 claims abstract description 8
- 241000251468 Actinopterygii Species 0.000 claims description 22
- 241001233037 catfish Species 0.000 claims description 19
- 238000006243 chemical reaction Methods 0.000 claims description 19
- 238000003756 stirring Methods 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 13
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- 239000000706 filtrate Substances 0.000 claims description 12
- 241000628997 Flos Species 0.000 claims description 11
- 230000008569 process Effects 0.000 claims description 11
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- 238000004821 distillation Methods 0.000 claims description 9
- 239000012153 distilled water Substances 0.000 claims description 9
- 238000002156 mixing Methods 0.000 claims description 9
- 238000002390 rotary evaporation Methods 0.000 claims description 8
- BLRPTPMANUNPDV-UHFFFAOYSA-N Silane Chemical compound [SiH4] BLRPTPMANUNPDV-UHFFFAOYSA-N 0.000 claims description 6
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 6
- 238000003763 carbonization Methods 0.000 claims description 6
- 239000000919 ceramic Substances 0.000 claims description 6
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 239000012467 final product Substances 0.000 claims description 6
- 239000012299 nitrogen atmosphere Substances 0.000 claims description 6
- 239000011347 resin Substances 0.000 claims description 6
- 229920005989 resin Polymers 0.000 claims description 6
- 229910000077 silane Inorganic materials 0.000 claims description 6
- 238000013517 stratification Methods 0.000 claims description 6
- 235000011149 sulphuric acid Nutrition 0.000 claims description 6
- 239000001117 sulphuric acid Substances 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 238000009777 vacuum freeze-drying Methods 0.000 claims description 6
- 239000003643 water by type Substances 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 5
- 230000008859 change Effects 0.000 claims description 4
- 235000019476 oil-water mixture Nutrition 0.000 claims description 3
- 239000000126 substance Substances 0.000 abstract description 11
- 230000000694 effects Effects 0.000 abstract description 9
- 244000005700 microbiome Species 0.000 abstract description 3
- 231100000252 nontoxic Toxicity 0.000 abstract description 3
- 230000003000 nontoxic effect Effects 0.000 abstract description 3
- 239000007822 coupling agent Substances 0.000 abstract description 2
- 230000007547 defect Effects 0.000 abstract description 2
- 239000000203 mixture Substances 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 239000010634 clove oil Substances 0.000 abstract 4
- 239000012141 concentrate Substances 0.000 abstract 2
- 239000011148 porous material Substances 0.000 abstract 2
- 238000004321 preservation Methods 0.000 abstract 2
- 241000252203 Clupea harengus Species 0.000 abstract 1
- 235000019514 herring Nutrition 0.000 abstract 1
- 230000001681 protective effect Effects 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 8
- 238000003860 storage Methods 0.000 description 8
- 230000000844 anti-bacterial effect Effects 0.000 description 7
- 230000002421 anti-septic effect Effects 0.000 description 5
- 235000016709 nutrition Nutrition 0.000 description 5
- 230000035764 nutrition Effects 0.000 description 5
- 241000193155 Clostridium botulinum Species 0.000 description 4
- 238000005260 corrosion Methods 0.000 description 4
- 239000000796 flavoring agent Substances 0.000 description 4
- 235000019634 flavors Nutrition 0.000 description 4
- 239000002932 luster Substances 0.000 description 4
- 238000007789 sealing Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- WPYMKLBDIGXBTP-UHFFFAOYSA-N benzoic acid Chemical compound OC(=O)C1=CC=CC=C1 WPYMKLBDIGXBTP-UHFFFAOYSA-N 0.000 description 3
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 230000002349 favourable effect Effects 0.000 description 3
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 description 2
- 239000004599 antimicrobial Substances 0.000 description 2
- BEFDCLMNVWHSGT-UHFFFAOYSA-N ethenylcyclopentane Chemical compound C=CC1CCCC1 BEFDCLMNVWHSGT-UHFFFAOYSA-N 0.000 description 2
- RRAFCDWBNXTKKO-UHFFFAOYSA-N eugenol Chemical compound COC1=CC(CC=C)=CC=C1O RRAFCDWBNXTKKO-UHFFFAOYSA-N 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 235000010241 potassium sorbate Nutrition 0.000 description 2
- 239000004302 potassium sorbate Substances 0.000 description 2
- 229940069338 potassium sorbate Drugs 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 235000010199 sorbic acid Nutrition 0.000 description 2
- 239000004334 sorbic acid Substances 0.000 description 2
- 229940075582 sorbic acid Drugs 0.000 description 2
- 230000008359 toxicosis Effects 0.000 description 2
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- 241000228245 Aspergillus niger Species 0.000 description 1
- 241000193755 Bacillus cereus Species 0.000 description 1
- 241000194108 Bacillus licheniformis Species 0.000 description 1
- 241000194107 Bacillus megaterium Species 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 239000005711 Benzoic acid Substances 0.000 description 1
- BCZXFFBUYPCTSJ-UHFFFAOYSA-L Calcium propionate Chemical compound [Ca+2].CCC([O-])=O.CCC([O-])=O BCZXFFBUYPCTSJ-UHFFFAOYSA-L 0.000 description 1
- NPBVQXIMTZKSBA-UHFFFAOYSA-N Chavibetol Natural products COC1=CC=C(CC=C)C=C1O NPBVQXIMTZKSBA-UHFFFAOYSA-N 0.000 description 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 1
- 241001478240 Coccus Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000194032 Enterococcus faecalis Species 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 239000005770 Eugenol Substances 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- QUOGESRFPZDMMT-UHFFFAOYSA-N L-Homoarginine Natural products OC(=O)C(N)CCCCNC(N)=N QUOGESRFPZDMMT-UHFFFAOYSA-N 0.000 description 1
- QUOGESRFPZDMMT-YFKPBYRVSA-N L-homoarginine Chemical compound OC(=O)[C@@H](N)CCCCNC(N)=N QUOGESRFPZDMMT-YFKPBYRVSA-N 0.000 description 1
- 240000006024 Lactobacillus plantarum Species 0.000 description 1
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- UVMRYBDEERADNV-UHFFFAOYSA-N Pseudoeugenol Natural products COC1=CC(C(C)=C)=CC=C1O UVMRYBDEERADNV-UHFFFAOYSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 235000009754 Vitis X bourquina Nutrition 0.000 description 1
- 235000012333 Vitis X labruscana Nutrition 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 235000014787 Vitis vinifera Nutrition 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000000845 anti-microbial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 235000010233 benzoic acid Nutrition 0.000 description 1
- 229960004365 benzoic acid Drugs 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 235000010331 calcium propionate Nutrition 0.000 description 1
- 239000004330 calcium propionate Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 235000013351 cheese Nutrition 0.000 description 1
- 239000000460 chlorine Substances 0.000 description 1
- 229910052801 chlorine Inorganic materials 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 229960002217 eugenol Drugs 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 231100001261 hazardous Toxicity 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 229940072205 lactobacillus plantarum Drugs 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 description 1
- 235000010234 sodium benzoate Nutrition 0.000 description 1
- 239000004299 sodium benzoate Substances 0.000 description 1
- 229960003885 sodium benzoate Drugs 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3472—Compounds of undetermined constitution obtained from animals or plants
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/358—Inorganic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Inorganic Chemistry (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention discloses a preparation method of a preservative for biomass food and belongs to the technical field of food preservative preparations. Clove oil is extracted for a standby application, then the clove oil is mixed with fishbones, propolis and pig manure, the mixture is subjected to a fermentation, micro-organisms are utilized to conduct a mild rotten treatment of the fishbones to enable the surface of the fishbones to be covered with micro-pores, then a protamine concentrate is extracted from herring spermaries, and the protamine concentrate is reacted with the prepared clove oil and micro-porous fishbone charcoal under the action of a coupling agent to prepare the preservative. The clove oil and protamine having broad spectrum bacteriostasis are used as preservative substances, and then the fishbone charcoal with a huge specific surface area is used to absorb the preservative substances, so that the preservative substances are loaded in the internal pores of the micro-porous fishbone charcoal to play protective and slow release effects. Besides, the used raw materials are natural and non-toxic, and the preservative is long in validity period of the preservation, solves the problems that the traditional chemical preservatives are harmful to the human body and the environment, also makes up the defects of short food shelf life caused by the poor preservation effect, and has very excellent application prospects.
Description
Technical field
The present invention relates to a kind of preparation method of biomass food preservative, belongs to food preservative preparing technical field.
Background technology
Food preservative is the corrupt medicament of inhibiting substances.Growth i.e. to the microorganism with corrupt substance as metabolism substrate
With lasting inhibitory action.It is important that it can suppress most incident Putrefaction in varied situations, particularly one
As sterilization functions it is insufficient when still with persistence effect.
China specify using preservative have 30 kinds of benzoic acid, sodium benzoate, sorbic acid, potassium sorbate, calcium propionate etc..But
It is that, based on chemical preservative, often containing a large amount of harmful materials, such as benzoate may for existing preservative
Alimentary toxicosis phenomenon, sorbic acid and potassium sorbate can be caused to be safe preservative generally acknowledged in the world, but Excess free enthalpy also can shadow
The balance of human metabolism is rung, also some preservative may cause cancer, cause deformity or cause alimentary toxicosis etc., this
Outward, existing preservative antiseptic effect is poor, causes the storage life of food shorter, it is difficult to meet the market demand.
Protamine is find in fish sperm cell a kind of tiny and the simple strong basicity albumen containing homoarginine
Matter, it is to bacillus subtilis, bacillus megaterium, bacillus licheniformis, solidification bacillus cereuss, Lactobacillus plantarum, cheese breast bar
Bacterium, streptococcus faecalis etc. have compared with high inhibition effect, but unobvious to gram negative bacteria inhibition.Research discovery, milt
Albumen can be acted on some protein for being related to nutrition transport or biosynthesis system in cell membrane, make the function of these protein
It is impaired, and then suppress the metabolism of cell and make cell death.Antibacterial effect of the protamine in neutral and alkaline medium
It is more notable.
Essential oil is contained in the extract of root, bark, seed or fruit of the fragrant plant in the torrid zone, always people's ratio
One of natural antiseptic agent interested.Containing eugenol, tannin etc. in Oleum Caryophylli.Research finds that Oleum Caryophylli is to golden yellow Portugal
The food such as grape coccus, escherichia coli, yeast, aspergillus niger have broad-spectrum antibacterial to act on, and to thermally-stabilised, its protrusion within 100 DEG C
Feature is that Fungistasis are strong.
The content of the invention
The technical problem to be solved：It is poor for current chemical preservative antiseptic effect, and containing a large amount of to people
The harmful material of body and environment, causes shelf life storage shorter, the drawbacks of being detrimental to health is eaten for a long time, there is provided Yi Zhongti
Take Oleum Caryophylli standby, then by fish spicule, propolis and pig manure mixed fermentation, using microorganism rotten process micro- to fish spicule so as to surface cloth
Full small hole, then protamine concentrated solution is extracted from catfish spermary, with standby Oleum Caryophylli and micro- in the presence of coupling agent
Hole fish spicule charcoal reaction, is obtained the method for preservative.The present invention using have broad-spectrum antibacterial Oleum Caryophylli and protamine as
Preservative substance, then with the fish spicule charcoal with huge specific surface area adsorbing preservative substance so as to it is supported on inside micropore fish spicule charcoal
In hole, protection and slow releasing function, and raw materials Nantural non-toxic are played, anti-corrosion effect duration is long, has both solved traditional chemical
The preservative problem harmful to human body and environment, compensate for antiseptic effect difference again causes the short defect of shelf life storage, with pole
Good application prospect.
To solve above-mentioned technical problem, the present invention using technical scheme as described below is：
（1）Weigh 500～600g Flos Caryophyllis, with grain crusher crush 10～15min after, be placed in distilling flask, by Flos Caryophylli with
Distilled water mass ratio is 1:3, distilled water is added in flask, and connects condensing tube, 20～30min of ebuillition of heated collects distillation
The oil water mixture for obtaining, is gone in separatory funnel, 20～30min of stratification, isolated upper strata Oleum Caryophylli, standby
With；
（2）500～600g fish spicules, 200～300g propolis, 700～800g pig manures and 2～3L deionized waters are weighed respectively, are mixed
After be put in ceramic fermentation tank, temperature be 30～40 DEG C, relative air humidity be 60～70% environment under, the micro- corruption of natural fermentation
Process 7～9 days, process terminates rear deionized water and rinses 20～30min, obtain the fish spicule after the micro- corruption in surface, put it into charcoal
Change in stove, under nitrogen atmosphere, with 200～300 DEG C of temperature 2～3h of carbonization, obtain micropore fish spicule charcoal, it is standby；
（3）300～400g catfish spermaries are weighed, 20～30min is crushed in homogenate in being put into tissue refiner, by the homogenate for obtaining
After being the mixing of 0.3mol/L sulphuric acid with concentration, 1～2h of acidolysis reaction, then addition 900～1000mL concentration is in acidolysis thing
0.1mol/L sodium chloride solutions, after 1～3h of stirring reaction, obtain filtrate with 3 layers of filtered through gauze；
（4）It is 1 in mass ratio:3, in above-mentioned filtrate, add mass fraction to be 70% ethanol solution, after 15～20min of stirring, use
Centrifuge puts it into rotary evaporation with 2000～3000r/min rotating speed 10～15min of centrifugal treating, isolated supernatant
In instrument, concentrated by rotary evaporation obtains catfish protamine concentrated solution to the 1/3 of its original volume；
（5）Count by weight, weigh 20～30 parts of steps（1）Standby Oleum Caryophylli, 30～40 parts of steps（2）Standby micropore
Fish spicule charcoal, 50～60 parts of above-mentioned catfish protamine concentrated solutions and 3～5 parts of silane resin acceptor kh-550s, are sequentially placed into reactor
In, after 3～5h of stirring reaction at 40～50 DEG C, be filtrated to get filtering residue, with vacuum freeze-drying machine lyophilizing after, obtain final product biomass food
Preservative.
The application process of the present invention：Food preservative obtained in the present invention is added into food pack, addition is
The 0.2～0.5% of food gross mass, addition is finished, and stands sealing.After testing, food preservative antiseptic effect of the present invention
Good, broad spectrum antibacterial is strong, can effectively prevent the growth of the harmful microbes such as bacillus botulinus and breed, extend shelf life storage 10～
15 days, and the original nutrition of food, local flavor and color and luster can be effectively kept, it is worthy to be popularized and use.
Compared with additive method, Advantageous Effects are the present invention：
（1）Preparation process of the present invention is simple, and gained food preservative has preferable antiseptic effect and stronger anti-microbial property, can
Effectively the pathogenic bacterium in suppression food and putrefaction bacteria, extend shelf life storage 10～15 days；
（2）Food preservative safety and environmental protection of the present invention is nontoxic, good stability, to health non-hazardous.
Specific embodiment
500～600g Flos Caryophyllis are weighed first, are crushed after 10～15min with grain crusher, are placed in distilling flask, by fourth
Fragrant is 1 with distilled water mass ratio:3, distilled water is added in flask, and connects condensing tube, 20～30min of ebuillition of heated is collected
The oil water mixture that distillation is obtained, is gone in separatory funnel, 20～30min of stratification, isolated upper strata Oleum Caryophylli,
It is standby；Weigh 500～600g fish spicules, 200～300g propolis, 700～800g pig manures and 2～3L deionized waters again respectively, mix
After be put in ceramic fermentation tank, temperature be 30～40 DEG C, relative air humidity be 60～70% environment under, the micro- corruption of natural fermentation
Process 7～9 days, process terminates rear deionized water and rinses 20～30min, obtain the fish spicule after the micro- corruption in surface, put it into charcoal
Change in stove, under nitrogen atmosphere, with 200～300 DEG C of temperature 2～3h of carbonization, obtain micropore fish spicule charcoal, it is standby；Then weigh
300～400g catfish spermaries, homogenate in being put into tissue refiner crush 20～30min, by the homogenate for obtaining and concentration are
After the mixing of 0.3mol/L sulphuric acid, 1～2h of acidolysis reaction, then in acidolysis thing, add 900～1000mL concentration to be 0.1mol/L chlorine
Change sodium solution, after 1～3h of stirring reaction, filtrate is obtained with 3 layers of filtered through gauze；It is 1 in mass ratio:3, add in above-mentioned filtrate
Mass fraction is 70% ethanol solution, after 15～20min of stirring, with centrifuge with 2000～3000r/min rotating speeds centrifugal treating 10
～15min, isolated supernatant, in putting it into Rotary Evaporators, concentrated by rotary evaporation obtains catfish fish to the 1/3 of its original volume
Protamine concentrated solution；Finally count by weight, weigh 20～30 portions of standby Oleum Caryophyllis, 30～40 parts of standby micropore fish spicules
Charcoal, 50～60 parts of above-mentioned catfish protamine concentrated solutions and 3～5 parts of silane resin acceptor kh-550s, are sequentially placed in reactor,
At 40～50 DEG C after 3～5h of stirring reaction, be filtrated to get filtering residue, with vacuum freeze-drying machine lyophilizing after, obtain final product biomass food antiseptic
Agent.
Example 1
500g Flos Caryophyllis are weighed first, is crushed after 10min with grain crusher, is placed in distilling flask, by Flos Caryophylli and distillation water quality
Amount is than being 1:3, distilled water is added in flask, and connects condensing tube, ebuillition of heated 20min, the profit that collection distillation is obtained are mixed
Compound, is gone in separatory funnel, stratification 20min, isolated upper strata Oleum Caryophylli, standby；Weigh 500g again respectively
Fish spicule, 200g propolis, 700g pig manures and 2L deionized waters, are put into after mixing in ceramic fermentation tank, are 30 DEG C, air phase in temperature
It is the micro- rotten process of natural fermentation 7 days under 60% environment to humidity, process terminates rear deionized water and rinses 20min, obtains surface
Fish spicule after micro- corruption, puts it in retort, under nitrogen atmosphere, with 200 DEG C of temperature carbonization 2h, obtains micropore fish spicule
Charcoal, it is standby；Then 300g catfish spermaries are weighed, homogenate in tissue refiner is put into and is crushed 20min, by the homogenate for obtaining and dense
Spend after mixing for 0.3mol/L sulphuric acid, acidolysis reaction 1h, then in acidolysis thing, add 900mL concentration to be that 0.1mol/L Sodium Chloride is molten
Liquid, after stirring reaction 1h, obtains filtrate with 3 layers of filtered through gauze；It is 1 in mass ratio:3, mass fraction is added in above-mentioned filtrate
For 70% ethanol solution, after stirring 15min, with centrifuge with 2000r/min rotating speed centrifugal treating 10min, isolated supernatant
Liquid, in putting it into Rotary Evaporators, concentrated by rotary evaporation obtains catfish protamine concentrated solution to the 1/3 of its original volume；Finally press
Parts by weight meter, weighs 20 portions of standby Oleum Caryophyllis, 30 parts of standby micropore fish spicule charcoals, 50 parts of above-mentioned catfish protamine concentrations
Liquid and 3 parts of silane resin acceptor kh-550s, are sequentially placed in reactor, after stirring reaction 3h at 40 DEG C, are filtrated to get filtering residue, use
After vacuum freeze-drying machine lyophilizing, biomass food preservative is obtained final product.
Food preservative obtained in the present invention is added into food pack, addition for food gross mass 0.2%,
Addition is finished, and stands sealing.After testing, food preservative favorable anti-corrosion effect of the present invention, broad spectrum antibacterial are strong, effectively can prevent
Only the growth of the harmful microbe such as bacillus botulinus and breeding, extend shelf life storage 10 days, and can effectively keep food original
Nutrition, local flavor and color and luster, are worthy to be popularized and use.
Example 2
550g Flos Caryophyllis are weighed first, is crushed after 13min with grain crusher, is placed in distilling flask, by Flos Caryophylli and distillation water quality
Amount is than being 1:3, distilled water is added in flask, and connects condensing tube, ebuillition of heated 25min, the profit that collection distillation is obtained are mixed
Compound, is gone in separatory funnel, stratification 25min, isolated upper strata Oleum Caryophylli, standby；Weigh 550g again respectively
Fish spicule, 250g propolis, 750g pig manures and 3L deionized waters, are put into after mixing in ceramic fermentation tank, are 35 DEG C, air phase in temperature
It is the micro- rotten process of natural fermentation 8 days under 65% environment to humidity, process terminates rear deionized water and rinses 25min, obtains surface
Fish spicule after micro- corruption, puts it in retort, under nitrogen atmosphere, with 250 DEG C of temperature carbonization 3h, obtains micropore fish spicule
Charcoal, it is standby；Then 350g catfish spermaries are weighed, homogenate in tissue refiner is put into and is crushed 25min, by the homogenate for obtaining and dense
Spend after mixing for 0.3mol/L sulphuric acid, acidolysis reaction 2h, then in acidolysis thing, add 950mL concentration to be that 0.1mol/L Sodium Chloride is molten
Liquid, after stirring reaction 2h, obtains filtrate with 3 layers of filtered through gauze；It is 1 in mass ratio:3, mass fraction is added in above-mentioned filtrate
For 70% ethanol solution, after stirring 18min, with centrifuge with 2500r/min rotating speed centrifugal treating 13min, isolated supernatant
Liquid, in putting it into Rotary Evaporators, concentrated by rotary evaporation obtains catfish protamine concentrated solution to the 1/3 of its original volume；Finally press
Parts by weight meter, weighs 25 portions of standby Oleum Caryophyllis, 35 parts of standby micropore fish spicule charcoals, 55 parts of above-mentioned catfish protamine concentrations
Liquid and 4 parts of silane resin acceptor kh-550s, are sequentially placed in reactor, after stirring reaction 4h at 45 DEG C, are filtrated to get filtering residue, use
After vacuum freeze-drying machine lyophilizing, biomass food preservative is obtained final product.
Food preservative obtained in the present invention is added into food pack, addition for food gross mass 0.4%,
Addition is finished, and stands sealing.After testing, food preservative favorable anti-corrosion effect of the present invention, broad spectrum antibacterial are strong, effectively can prevent
Only the growth of the harmful microbe such as bacillus botulinus and breeding, extend shelf life storage 13 days, and can effectively keep food original
Nutrition, local flavor and color and luster, are worthy to be popularized and use.
Example 3
600g Flos Caryophyllis are weighed first, is crushed after 15min with grain crusher, is placed in distilling flask, by Flos Caryophylli and distillation water quality
Amount is than being 1:3, distilled water is added in flask, and connects condensing tube, ebuillition of heated 30min, the profit that collection distillation is obtained are mixed
Compound, is gone in separatory funnel, stratification 30min, isolated upper strata Oleum Caryophylli, standby；Weigh 600g again respectively
Fish spicule, 300g propolis, 800g pig manures and 3L deionized waters, are put into after mixing in ceramic fermentation tank, are 40 DEG C, air phase in temperature
It is the micro- rotten process of natural fermentation 9 days under 70% environment to humidity, process terminates rear deionized water and rinses 30min, obtains surface
Fish spicule after micro- corruption, puts it in retort, under nitrogen atmosphere, with 300 DEG C of temperature carbonization 3h, obtains micropore fish spicule
Charcoal, it is standby；Then 400g catfish spermaries are weighed, homogenate in tissue refiner is put into and is crushed 30min, by the homogenate for obtaining and dense
Spend after mixing for 0.3mol/L sulphuric acid, acidolysis reaction 2h, then in acidolysis thing, add 1000mL concentration to be 0.1mol/L Sodium Chloride
Solution, after stirring reaction 3h, obtains filtrate with 3 layers of filtered through gauze；It is 1 in mass ratio:3, quality point is added in above-mentioned filtrate
Number is 70% ethanol solution, after stirring 20min, with centrifuge with 3000r/min rotating speed centrifugal treating 15min, isolated supernatant
Liquid, in putting it into Rotary Evaporators, concentrated by rotary evaporation obtains catfish protamine concentrated solution to the 1/3 of its original volume；Finally press
Parts by weight meter, weighs 30 portions of standby Oleum Caryophyllis, 40 parts of standby micropore fish spicule charcoals, 60 parts of above-mentioned catfish protamine concentrations
Liquid and 5 parts of silane resin acceptor kh-550s, are sequentially placed in reactor, after stirring reaction 5h at 50 DEG C, are filtrated to get filtering residue, use
After vacuum freeze-drying machine lyophilizing, biomass food preservative is obtained final product.
Food preservative obtained in the present invention is added into food pack, addition for food gross mass 0.5%,
Addition is finished, and stands sealing.After testing, food preservative favorable anti-corrosion effect of the present invention, broad spectrum antibacterial are strong, effectively can prevent
Only the growth of the harmful microbe such as bacillus botulinus and breeding, extend shelf life storage 15 days, and can effectively keep food original
Nutrition, local flavor and color and luster, are worthy to be popularized and use.
Claims (1)
1. a kind of preparation method of biomass food preservative, it is characterised in that concrete preparation process is：
（1）Weigh 500～600g Flos Caryophyllis, with grain crusher crush 10～15min after, be placed in distilling flask, by Flos Caryophylli with
Distilled water mass ratio is 1:3, distilled water is added in flask, and connects condensing tube, 20～30min of ebuillition of heated collects distillation
The oil water mixture for obtaining, is gone in separatory funnel, 20～30min of stratification, isolated upper strata Oleum Caryophylli, standby
With；
（2）500～600g fish spicules, 200～300g propolis, 700～800g pig manures and 2～3L deionized waters are weighed respectively, are mixed
After be put in ceramic fermentation tank, temperature be 30～40 DEG C, relative air humidity be 60～70% environment under, the micro- corruption of natural fermentation
Process 7～9 days, process terminates rear deionized water and rinses 20～30min, obtain the fish spicule after the micro- corruption in surface, put it into charcoal
Change in stove, under nitrogen atmosphere, with 200～300 DEG C of temperature 2～3h of carbonization, obtain micropore fish spicule charcoal, it is standby；
（3）300～400g catfish spermaries are weighed, 20～30min is crushed in homogenate in being put into tissue refiner, by the homogenate for obtaining
After being the mixing of 0.3mol/L sulphuric acid with concentration, 1～2h of acidolysis reaction, then addition 900～1000mL concentration is in acidolysis thing
0.1mol/L sodium chloride solutions, after 1～3h of stirring reaction, obtain filtrate with 3 layers of filtered through gauze；
（4）It is 1 in mass ratio:3, in above-mentioned filtrate, add mass fraction to be 70% ethanol solution, after 15～20min of stirring, use
Centrifuge puts it into rotary evaporation with 2000～3000r/min rotating speed 10～15min of centrifugal treating, isolated supernatant
In instrument, concentrated by rotary evaporation obtains catfish protamine concentrated solution to the 1/3 of its original volume；
（5）Count by weight, weigh 20～30 parts of steps（1）Standby Oleum Caryophylli, 30～40 parts of steps（2）Standby micropore
Fish spicule charcoal, 50～60 parts of above-mentioned catfish protamine concentrated solutions and 3～5 parts of silane resin acceptor kh-550s, are sequentially placed into reactor
In, after 3～5h of stirring reaction at 40～50 DEG C, be filtrated to get filtering residue, with vacuum freeze-drying machine lyophilizing after, obtain final product biomass food
Preservative.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101755994A (en) * | 2008-12-11 | 2010-06-30 | 湖州来色生物基因工程有限公司 | Preparation method of natural food preservative of protamine |
| CN103936846A (en) * | 2014-04-30 | 2014-07-23 | 上海第一生化药业有限公司 | Purification method of protamine sulfate |
| CN104738787A (en) * | 2015-04-17 | 2015-07-01 | 黄琳 | Natural preservative and preparation method thereof |
| CN105054219A (en) * | 2015-09-18 | 2015-11-18 | 四川省汇泉罐头食品有限公司 | Compound food preservative |
-
2016
- 2016-11-02 CN CN201610945110.4A patent/CN106509586A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101755994A (en) * | 2008-12-11 | 2010-06-30 | 湖州来色生物基因工程有限公司 | Preparation method of natural food preservative of protamine |
| CN103936846A (en) * | 2014-04-30 | 2014-07-23 | 上海第一生化药业有限公司 | Purification method of protamine sulfate |
| CN104738787A (en) * | 2015-04-17 | 2015-07-01 | 黄琳 | Natural preservative and preparation method thereof |
| CN105054219A (en) * | 2015-09-18 | 2015-11-18 | 四川省汇泉罐头食品有限公司 | Compound food preservative |
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