CN103976015B - A kind of antistaling agent containing polyaminopropyl biguanide for the control of oranges and tangerines blossom-end rot and Synthesis and applications thereof - Google Patents

A kind of antistaling agent containing polyaminopropyl biguanide for the control of oranges and tangerines blossom-end rot and Synthesis and applications thereof Download PDF

Info

Publication number
CN103976015B
CN103976015B CN201410202170.8A CN201410202170A CN103976015B CN 103976015 B CN103976015 B CN 103976015B CN 201410202170 A CN201410202170 A CN 201410202170A CN 103976015 B CN103976015 B CN 103976015B
Authority
CN
China
Prior art keywords
polyaminopropyl biguanide
rot
antistaling agent
blossom
oranges
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201410202170.8A
Other languages
Chinese (zh)
Other versions
CN103976015A (en
Inventor
吴日章
袁永雁
温书恒
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zhenluse Technology Co Ltd Zhuhai City
Original Assignee
Zhenluse Technology Co Ltd Zhuhai City
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Zhenluse Technology Co Ltd Zhuhai City filed Critical Zhenluse Technology Co Ltd Zhuhai City
Priority to CN201410202170.8A priority Critical patent/CN103976015B/en
Publication of CN103976015A publication Critical patent/CN103976015A/en
Application granted granted Critical
Publication of CN103976015B publication Critical patent/CN103976015B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Abstract

The present invention relates to a kind of antistaling agent containing polyaminopropyl biguanide for the control of oranges and tangerines blossom-end rot and Synthesis and applications thereof, composition and the proportioning of described antistaling agent are as follows: polyaminopropyl biguanide 10-45 % by weight; Anticorrisive agent 5-15 % by weight; Bioactivator 2-10 % by weight; Emulsifying agent 10-20 % by weight; Surplus is water.This antistaling agent significantly can reduce the generation of citrusfruit postharvest disease, and control blossom-end rot evil, to the significant corrosion-resistanting fresh-keeping effect of oranges and tangerines tool, delayed fruit is old and feeble, the storage period of extending fruit and shelf life.Antistaling agent tool safety of the present invention, environmental protection, environmentally safe, preparation method is simple, integrated cost is low, and using method is flexible, convenient, is easy to the feature applied.

Description

A kind of antistaling agent containing polyaminopropyl biguanide for the control of oranges and tangerines blossom-end rot and Synthesis and applications thereof
Technical field
The invention belongs to preserving fruit and vegetable utilizing field, relate to the control of oranges and tangerines blossom-end rot, particularly a kind of antistaling agent containing polyaminopropyl biguanide for the control of oranges and tangerines blossom-end rot and Synthesis and applications thereof.
Background technology
Oranges and tangerines (citrusfruit) blossom-end rot is citrus later stage main disease of rotting, comprises the black blossom-end rot that the brown blossom-end rot that caused by Phomopsis (Phomopsisstem-endrot) and Diplodia (Diplodiastem-endrot) cause.Disease, mainly at Citrus Fruits In Storage late onset, starts from base of fruit or wound, extends to full fruit, the fruit browning or black after morbidity from local, and sick fruit pulp rots more rotten than pericarp fast, also known as " punching is rotten ", and easy cross-infection between oranges and tangerines.
The diseases such as the chemical bactericide (as 50% thiophanate methyl, 1500 ~ 2000 times of liquid or 50% carbendazol wettable powder, 2000 times of liquid) that rear anti-corrosive fresh-keeping the most generally uses is mould to viridescent, anthracnose adopted by oranges and tangerines good prevention effect, but then preventive effect is not good for blossom-end rot.The blossom-end rot incidence of disease has ascendant trend in recent years, but, correlative study not good to its preventive effect is few again, have impact on the industry development of oranges and tangerines.For this reason, from raising, oranges and tangerines blossom-end rot preventive effect, minimizing bactericide use amount, the reduction resistance to the action of a drug are produced, ensure food security angle, seek and develop effective, the safe material tool significance of oranges and tangerines blossom-end rot.
Polyaminopropyl biguanide is the one of biguanides, molecular formula: (C 5h 14n 6) X, be a kind of fungi-proofing material of mildew-resistant of high-efficiency broad spectrum safety.The characteristic of cation superpolymer, makes it optionally be adsorbed in pathogenic microorganisms surface, acts on cell membrane, destroys film and eucaryotic cell structure and then plays fungus and mildew resistance effect.Research shows polyaminopropyl biguanide 8500mg/L solution, and chmice acute LD50 of passing through mouth is greater than 5000mg/kg, and rat acute toxicity on inhalation LC50 (2h) is greater than 10000mg/m3, belongs to actual nontoxic level.In addition, the polyaminopropyl biguanide of 3-4ppm is perfectly safe to aquatile, environmentally safe, can naturally or by biological decomposition, when can by microbial digestion catabolism after high dilution.
Polyaminopropyl biguanide is mainly used in the surface sterilization of cosmetics, medicine, food processing both at home and abroad at present, does not have polyaminopropyl biguanide to be applied to citrus and adopts rear blossom-end rot research report or the listing of relevant matured product.
Therefore, a kind of antistaling agent containing polyaminopropyl biguanide for the control of oranges and tangerines blossom-end rot is provided just to become the technical barrier that this technical field is badly in need of solution.
Summary of the invention
An object of the present invention is to provide a kind of antistaling agent containing polyaminopropyl biguanide for the control of oranges and tangerines blossom-end rot.
Above-mentioned purpose of the present invention reaches by the following technical programs:
For the control of oranges and tangerines blossom-end rot containing the antistaling agent of polyaminopropyl biguanide, its composition and proportioning as follows:
Wherein, described anticorrisive agent is the mixing of one or more arbitrary proportions in citric acid, benzoate, parabens and sodium salt thereof or sodium Diacetate; Described bioactivator is the mixing of one or more arbitrary proportions in calcium chloride, Calcium Ascorbate, shitosan, chitosan oligosaccharide or oxalic acid.
Wherein, described emulsifying agent is polyethylene glycol, ethylene glycol, propane diols, glycerine, methyl glycol fatty acid ester, mono-fatty acid glyceride, diglycerine fatty acid ester, propylene glycol alginate, mono succinate glycerine, polyglyceryl fatty acid ester, Tween-20 (polysorbas20), polyoxyethylene sorbitol acid anhydride monopalmitate (polysorbate40), polyoxyethylene sorbitan monostearate (polysorbate60), Tween-80 (Tween 80), hydrogenated rosin glyceride, calcium lactate, sorbitanmonolaureate (span 20), sorbitol anhydride monopalmitate (span 40), sorbitan monostearate (sorbester p18), sorbitol anhydride three stearate (sorbester p38), sorbitan mono-oleic acid ester (sorbester p17), stearoyl lactate, CSL, OPEO, NPE, calcium dodecyl benzene sulfonate, the mixing of one or more arbitrary proportions in styrylphenol polyoxyethylene ether series or alkyl phenol formaldehyde resin polyoxyethylene ether.
Another object of the present invention is to provide a kind of preparation method of the above-mentioned antistaling agent containing polyaminopropyl biguanide for the control of oranges and tangerines blossom-end rot.
Above-mentioned purpose of the present invention reaches by the following technical programs:
For a preparation method for the antistaling agent containing polyaminopropyl biguanide of oranges and tangerines blossom-end rot control, its step is as follows:
(1) polyaminopropyl biguanide is added container in proportion, add suitable quantity of water, continuous heating, be stirred to dissolving, obtain polyaminopropyl biguanide solution;
(2) anticorrisive agent and bioactivator are added another container in proportion, add suitable quantity of water and dissolve, obtain the mixed solution of anticorrisive agent and bioactivator;
(3) mixed solution of step (2) gained anticorrisive agent and bioactivator is mixed with step (1) gained polyaminopropyl biguanide solution, add appropriate emulsifying agent again, mix, moisturizing to 100%, obtains the antistaling agent containing polyaminopropyl biguanide for the control of oranges and tangerines blossom-end rot.
Wherein, described anticorrisive agent is the mixing of one or more arbitrary proportions in citric acid, benzoate, parabens and sodium salt thereof or sodium Diacetate; Described bioactivator is the mixing of one or more arbitrary proportions in calcium chloride, Calcium Ascorbate, shitosan, chitosan oligosaccharide or oxalic acid.
Wherein, described emulsifying agent is polyethylene glycol, ethylene glycol, propane diols, glycerine, methyl glycol fatty acid ester, mono-fatty acid glyceride, diglycerine fatty acid ester, propylene glycol alginate, mono succinate glycerine, polyglyceryl fatty acid ester, Tween-20 (polysorbas20), polyoxyethylene sorbitol acid anhydride monopalmitate (polysorbate40), polyoxyethylene sorbitan monostearate (polysorbate60), Tween-80 (Tween 80), hydrogenated rosin glyceride, calcium lactate, sorbitanmonolaureate (span 20), sorbitol anhydride monopalmitate (span 40), sorbitan monostearate (sorbester p18), sorbitol anhydride three stearate (sorbester p38), sorbitan mono-oleic acid ester (sorbester p17), stearoyl lactate, CSL, OPEO, NPE, calcium dodecyl benzene sulfonate, the mixing of one or more arbitrary proportions in styrylphenol polyoxyethylene ether series or alkyl phenol formaldehyde resin polyoxyethylene ether.
Another object of the present invention is to provide a kind of application of the above-mentioned antistaling agent containing polyaminopropyl biguanide for the control of oranges and tangerines blossom-end rot.
Above-mentioned purpose of the present invention reaches by the following technical programs:
A kind of application of the antistaling agent containing polyaminopropyl biguanide after control oranges and tangerines are adopted in blossom-end rot for the control of oranges and tangerines blossom-end rot.
The concrete using method of the antistaling agent containing polyaminopropyl biguanide for the control of oranges and tangerines blossom-end rot of the present invention is as follows:
Freshly harvested or the fresh fruit of placement after 24 hours of gathering, remove mechanical damage, infect, the base of a fruit equivalent damage that falls, inferior quality fruit, tap water dries.By antistaling agent dilute with water 300-1500 of the present invention doubly, concrete required multiple is determined according to the concrete condition such as time of cost, required storage.Fresh fruit after gathering to be soaked in the citrus preservative diluted 1 ~ 3 minute, or directly to the face spraying of fruit fruit, until fruit face is completely moistening.Take out in room temperature, ventilation is spread out and is dried.Be that the polyethylene freshness protection package of 0.03mm carries out individual packing to fresh fruit after process with thickness, to keep moisture, reduce interacting and cross-infection after falling ill between fruit.By the fresh fruit after packaging, with light packs in leatheroid fruit case, preserve under being placed in room temperature or cryogenic conditions.
Composite preservative of the present invention also can be used for the control of adopting the storage Major Diseases such as rear Citrus green mold, sour rot, blossom-end rot, anthracnose, black rot, good to blossom-end rot preventive effect.
The present invention has following advantages and effect:
The antistaling agent containing polyaminopropyl biguanide for the control of oranges and tangerines blossom-end rot of the present invention is preparation method simple, the cost low antistaling agent for oranges and tangerines blossom-end rot control of comprehensive polyaminopropyl biguanide to the result of study exploitation that blossom-end rot is prevented and treated.This research shows that polyaminopropyl biguanide just effectively can suppress the growth of Stem-rot isolate at low concentrations, reduces the incidence of disease of citrus fruit body stalk maize ear rot, can be used for the control of oranges and tangerines blossom-end rot.Described for the control of oranges and tangerines blossom-end rot containing polyaminopropyl biguanide in the antistaling agent of polyaminopropyl biguanide to orange storing disease tool broad-spectrum antibacterial, particularly good to blossom-end rot preventive effect, can effectively remove fruit surface germ, and act on the germ hiding and contaminate, reduce disease and occur; Anticorrisive agent effects on surface, wound germ play detoxicating, bactericidal action, and the bacteria-treating effect contaminated hiding is weak, can work in coordination with polyaminopropyl biguanide and improve antistaling agent to the prevention effect of citrus disease.Bioactivator has selected tool to continue bioactivator that is old and feeble, induction of resistance, these materials can improve fruit disease resistance, the germ reproduction growth that suppressing hides contaminates, improve fruit oxidation resistance, delay senility process, thus reduce fruits nutrition material consumption, keep fruit freshness, edible quality.
The present invention has selected polyaminopropyl biguanide, foodstuff anticorrisive agent, biological activity to develop citrus preservative, has abandoned disinfectant use in agriculture completely, has guaranteed food security, environmental friendliness, the advantage such as tool green, safety, environmental protection.Using method flexibly, convenient, spray fresh-keeping before can be used for plucking, also can be used for post-harvest fruits and soak, or spray before adopting fresh-keeping soak with post-harvest fruits with the use of.
Below by the drawings and specific embodiments, the present invention will be further described, but and do not mean that limiting the scope of the invention.
Accompanying drawing explanation
Fig. 1 is that the polyaminopropyl biguanide of variable concentrations is to the tendency chart of Diplodia blossom-end rot mycelia Developing restraint.
Fig. 2-1 to Fig. 2-3 is that the polyaminopropyl biguanide of variable concentrations is to Diplodia blossom-end rot mycelia inhibition figure.
Fig. 3 is that the polyaminopropyl biguanide of variable concentrations is to the inhibiting tendency chart of precocious oranges and tangerines damage inoculation blossom-end rot.
Detailed description of the invention
Embodiment 1: containing the preparation of the antistaling agent 1 of polyaminopropyl biguanide
Preparation method: (1) takes the commercially available polyaminopropyl biguanide of 15g, is placed in the container with agitator, add water stirring 1 hour, dissolves, obtain polyaminopropyl biguanide solution; (2) in the container of another belt stirrer, add commercially available Sodium Benzoate 10g, commercially available calcium chloride 4g, adds suitable quantity of water, stirs 30 points, prepares uniform mixed liquor; (3) step (2) gained mixed liquor and the mixing of step (1) gained polyaminopropyl biguanide solution, add commercially available sorbitan mono-oleic acid ester 3g again, commercially available polyoxyethylene sorbitol acid anhydride 2g, commercially available propane diols 6g, mixing, moisturizing, to 100g, is stirred 30 minutes, fully emulsified mixing, products obtained therefrom is the antistaling agent 1 containing polyaminopropyl biguanide.
Gained is as follows containing the final proportioning of the antistaling agent 1 of polyaminopropyl biguanide:
Reference examples 1: containing the preparation of the contrast antistaling agent 1 of polyaminopropyl biguanide
Preparation method: (1) takes the commercially available polyaminopropyl biguanide of 15g, is placed in the container with agitator, add water stirring 1 hour, dissolves, obtain polyaminopropyl biguanide solution; (2) add commercially available sorbitan mono-oleic acid ester 3g again, commercially available polyoxyethylene sorbitol acid anhydride 2g, commercially available propane diols 6g, mixing, moisturizing, to 100g, is stirred 30 minutes, fully emulsified mixing, is obtained the contrast antistaling agent 1 containing polyaminopropyl biguanide.
The final proportioning of gained contrast antistaling agent 1 is as follows:
Embodiment 2: containing the preparation of the antistaling agent 2 of polyaminopropyl biguanide
Preparation method: (1) takes the commercially available polyaminopropyl biguanide of 30g, is placed in the container with agitator, add water stirring 1.5 hours, dissolves, obtain polyaminopropyl biguanide solution; (2) in the container of another belt stirrer, add commercially available sodium Diacetate 6g, commercially available Calcium Ascorbate 2.5g, adds suitable quantity of water, stirs 30 points, prepares uniform mixed liquor; (3) step (2) gained mixed liquor and the mixing of step (1) gained polyaminopropyl biguanide solution, add commercially available ethylene glycol 3g again, commercially available polyglyceryl fatty acid ester 4g, commercially available OPEO 5g, mixing, moisturizing, to 100g, is stirred 30 minutes, fully emulsified mixing, must containing the antistaling agent 2 of polyaminopropyl biguanide.
Under the final proportioning example of gained containing the antistaling agent 2 of polyaminopropyl biguanide:
Reference examples 2: containing the preparation of the contrast antistaling agent 2 of polyaminopropyl biguanide
Preparation method: (1) takes the commercially available polyaminopropyl biguanide of 30g, is placed in the container with agitator, add water stirring 1.5 hours, dissolves, obtain polyaminopropyl biguanide solution; (2) add commercially available ethylene glycol 3g again, commercially available polyglyceryl fatty acid ester 4g, commercially available OPEO 5g, mixing, moisturizing, to 100g, is stirred 30 minutes, fully emulsified mixing, must containing the contrast antistaling agent 2 of polyaminopropyl biguanide.
Gained is as follows containing the final proportioning of the contrast antistaling agent 2 of polyaminopropyl biguanide:
Embodiment 3: containing the preparation of the antistaling agent 3 of polyaminopropyl biguanide
Preparation method: (1) takes the commercially available polyaminopropyl biguanide of 45g, is placed in the container with agitator, add water stirring 1.5 hours, dissolves, obtain polyaminopropyl biguanide solution; (2) in the container of another belt stirrer, add commercially available citric acid 6g, shitosan 4g, adds suitable quantity of water, stirs 30 points, prepares uniform mixed liquor; (3) step (2) gained mixed liquor and the mixing of step (1) gained polyaminopropyl biguanide solution, add commercially available sorbitanmonolaureate 3g again, commercially available calcium lactate 4g, commercially available propane diols 4g, mixing, moisturizing, to 100g, is stirred 30 minutes, fully emulsified mixing, must containing the antistaling agent 3 of polyaminopropyl biguanide.
Gained is as follows containing the final proportioning of the antistaling agent 3 of polyaminopropyl biguanide:
Reference examples 3: containing the preparation of the contrast antistaling agent 3 of polyaminopropyl biguanide
Preparation method: (1) takes the commercially available polyaminopropyl biguanide of 45g, is placed in the container with agitator, add water stirring 1.5 hours, dissolves, obtain polyaminopropyl biguanide solution; (2) add commercially available sorbitanmonolaureate 3g again, commercially available calcium lactate 4g, commercially available propane diols 4g, mixing, moisturizing, to 100g, is stirred 30 minutes, fully emulsified mixing, must containing the contrast antistaling agent 3 of polyaminopropyl biguanide.
Gained is as follows containing the final proportioning of the contrast antistaling agent 3 of polyaminopropyl biguanide:
Embodiment 4: containing the preparation of the antistaling agent 4 of polyaminopropyl biguanide
Preparation method: (1) takes the commercially available polyaminopropyl biguanide of 10g, is placed in the container with agitator, add water stirring 1 hour, dissolves, obtain polyaminopropyl biguanide solution; (2) in the container of another belt stirrer, add commercially available nipagin A sodium 15g, commercially available chitosan oligosaccharide 5g, adds suitable quantity of water, stirs 30 points, prepares uniform mixed liquor; (3) step (2) gained mixed liquor and the mixing of step (1) gained polyaminopropyl biguanide solution, add commercially available NPE 8g again, commercially available calcium dodecyl benzene sulfonate 2g, commercially available propane diols 4g, commercially available glycerine 6g, mixing, moisturizing is to 100g, stir 30 minutes, fully emulsified mixing, must containing the antistaling agent 4 of polyaminopropyl biguanide.
Gained is as follows containing the final proportioning of the antistaling agent 4 of polyaminopropyl biguanide:
Reference examples 4: containing the preparation of the contrast antistaling agent 4 of polyaminopropyl biguanide
Preparation method: (1) takes the commercially available polyaminopropyl biguanide of 10g, is placed in the container with agitator, add water stirring 1 hour, dissolves, obtain polyaminopropyl biguanide solution; (2) add commercially available NPE 8g again, commercially available calcium dodecyl benzene sulfonate 2g, commercially available propane diols 4g, commercially available glycerine 6g, mixing, moisturizing, to 100g, is stirred 30 minutes, fully emulsified mixing, must containing the contrast antistaling agent 4 of polyaminopropyl biguanide.
Gained is as follows containing the final proportioning of the contrast antistaling agent 4 of polyaminopropyl biguanide:
Embodiment 5: containing the preparation of the antistaling agent 5 of polyaminopropyl biguanide
Preparation method: (1) takes the commercially available polyaminopropyl biguanide of 20g, is placed in the container with agitator, add water stirring 1 hour, dissolves, obtain polyaminopropyl biguanide solution; (2) in the container of another belt stirrer, add commercially available Sodium Methyl Hydroxybenzoate 10g, commercially available shitosan 4g, commercially available oxalic acid 6g, adds suitable quantity of water, stirs 30 points, prepares uniform mixed liquor; (3) step (2) gained mixed liquor and the mixing of step (1) gained polyaminopropyl biguanide solution, add commercially available propylene glycol alginate 4g again, the stearic 6g of commercially available polyoxyethylene sorbitol acid anhydride list, commercially available ethylene glycol 4g, mixing, moisturizing, to 100g, is stirred 30 minutes, fully emulsified mixing, must containing the antistaling agent 5 of polyaminopropyl biguanide.
Gained is as follows containing the final proportioning of the antistaling agent 5 of polyaminopropyl biguanide:
Reference examples 5: containing the preparation of the contrast antistaling agent 5 of polyaminopropyl biguanide
Preparation method: (1) takes the commercially available polyaminopropyl biguanide of 20g, is placed in the container with agitator, add water stirring 1 hour, dissolves, obtain polyaminopropyl biguanide solution; (2) add commercially available propylene glycol alginate 4g again, commercially available polyoxyethylene sorbitol acid anhydride list stearic 6g, commercially available ethylene glycol 4g, mixing, moisturizing, to 100g, is stirred 30 minutes, fully emulsified mixing, must containing the contrast antistaling agent 5 of polyaminopropyl biguanide.
Gained is as follows containing the final proportioning of the contrast antistaling agent 5 of polyaminopropyl biguanide:
Effect is tested:
1, polyaminopropyl biguanide is to blossom-end rot mycelia Cell suppression test
For proving that antistaling agent of the present invention is to the effect of oranges and tangerines blossom-end rot, by proving Diplodia (Diplodiastem-endrot) mycelial growth bacteriostatic experiment.Utilize case process citrusfruit, the method for natural occurrence rate observes the effect of antistaling agent of the present invention.
Fruit is the citrusfruit of normal commercial maturity, and test kind is that precocious tangerine is produced in Guangxi.Remove the fruit that mechanical damage, infection, size and the large first-class exterior quality of maturity are inconsistent, with tap water, at room temperature (20-25 DEG C) dries rear for subsequent use.
Diplodia (Diplodiastem-endrot) is for preserving bacterial classification in laboratory.Diplodia (Diplodiastem-endrot) is black blossom-end rot pathogenic bacteria, is located away from the citrusfruit of morbidity, through colonial morphology, microscopic examination and tieback experimental verification.Before experiment, be inoculated in the activation of PDA culture medium, expand, bacteria used thereby is the Diplodia (Diplodiastem-endrot) that PDA cultivates 6-8 days.
Antibacterial Activity (mycelial growth rate method):
The polyaminopropyl biguanide sample sterilized water of variable concentrations is mixed with variable concentrations, get 1ml respectively to join 9ml and melted and be cooled in the PDA culture medium of 50 DEG C, in the culture dish (Ф=90mm) of the abundant mixing sterilizing of falling people afterwards, make the pastille culture medium of series concentration, dull and stereotyped for contrast with the PDA adding equal-volume sterilized water.Buy the bacterium cake of cut-off footpath 5mm from PDA cultivation each bacterial strain colony edge of 4-6 days, mycelia faces down, and is inoculated in the pastille PDA culture medium central authorities of having solidified, is placed in dark culturing in 28 DEG C of constant incubators.Each process repeats for 5 times, test repetition 2 times.Within 5 days, measure afterwards for the examination colony diameter of germ on variable concentrations pastille culture medium, calculate the inhibiting rate of each chemicals treatment to mycelia simple interest with comparing.According to biometrics probability value conversion table, inhibition percentage is converted into and suppresses probability value.With the log concentration set in test as abscissa, suppress probability value to be ordinate, calculate toxicity regression linear equation and the EC EC50 of polyaminopropyl biguanide.
Damage inoculation method:
During inoculation, fruit surface first with 70% alcohol wipe sterilization, then uses sterilizing iron nail at the equidistant acanthopore in position, fruit equator 3 (dark 5mm, diameter 5mm), then it soaked 1 minute in the polyaminopropyl biguanide of setting concentration, contrast clear water soaks, and naturally dries after taking out.The bacterium cake beating cut-off footpath 5mm from PDA cultivation each bacterial strain colony edge of 4-6 days that access has been got ready, in access aperture, then place 10-12 hour, load in carton, sealing, in room temperature 20-25 DEG C, relative humidity RH preserves under 80 ~ 95% conditions.Often process repetition 3 times.Often process 30 citrusfruits.
Result is as follows:
Table 1
Table 1 is that the polyaminopropyl biguanide of variable concentrations is on the tables of data of the impact of Diplodia (Diplodiastem-endrot) blossom-end rot mycelial growth.
According to this table Plotting data tendency chart, obtain Fig. 1: the polyaminopropyl biguanide of variable concentrations is to the tendency chart of Diplodia (Diplodiastem-endrot) blossom-end rot mycelia Developing restraint.
Simultaneously to blank group 0mg/L (blank), concentration be 25mg/L, concentration is that the result of two test group of 50mg/L is taken pictures, and obtains Fig. 2-1, Fig. 2-2 and Fig. 2-3 respectively: the polyaminopropyl biguanide of variable concentrations is to Diplodia (Diplodiastem-endrot) blossom-end rot mycelia inhibition figure.
Can find out according to Fig. 1, when the concentration of polyaminopropyl biguanide is 10mg/L, be 43.27% to the bacteriostasis rate of Stem-rot isolate; Concentration is larger, larger to the bacteriostasis of blossom-end rot, concentration and being proportionate property of bacteriostasis rate, and when concentration reaches 50mg/L, bacteriostasis rate 92.86%, effectively can control the primary length of blossom-end rot.By statistical analysis, known polyaminopropyl biguanide is 14.04 to Stem-rot isolate to EC50, shows that polyaminopropyl biguanide well can suppress the growth of blossom-end rot mycelia, the using value huge to the anti-tool of oranges and tangerines blossom-end rot.
2, the polyaminopropyl biguanide of variable concentrations is tested the control and application of blossom-end rot
As shown in Figure 3, the polyaminopropyl biguanide of variable concentrations is to the inhibiting tendency chart of oranges and tangerines damage inoculation blossom-end rot, citrusfruit damage inoculation is after 4 days, the control group incidence of disease is 100%, and the oranges and tangerines blossom-end rot incidence of disease of the polyaminopropyl biguanide process of variable concentrations is all remarkable in contrast (P ﹤ 0.05); Between variable concentrations, concentration is higher, and the incidence of disease is lower, more obvious to the rotten bacterium inhibitory action of the base of a fruit.Between 400ppm and 600ppm process, blossom-end rot incidence of disease difference is maximum, and difference extremely significantly (P ﹤ 0.01); Concentration is greater than the process of 600ppm, and the incidence of disease difference of closing between concentration is little, 1000ppm and 600ppm difference not obvious (P ﹥ 0.05), namely when working concentration is greater than 600ppm, continues to improve working concentration less on the impact of blossom-end rot prevention effect.
3, embodiment of the present invention antistaling agent and reference examples are to the prevention effect of oranges and tangerines blossom-end rot
With following method process citrusfruit:
To gather the oranges and tangerines fresh fruit of placement after 24 hours, remove mechanical damage, infect, the base of a fruit equivalent damage that falls, inferior quality fruit, tap water dries.Antistaling agent containing polyaminopropyl biguanide of the present invention is used water as 300 ~ 600 times of dilutions, after stirring.Fresh fruit is soaked in dilution 2 ~ 3 minutes, takes out in room temperature ventilation airing in 24 hours.Be that the polyethylene freshness protection package of 0.03mm carries out individual packing to fresh fruit after process with thickness, to keep moisture, reduce interacting and cross-infection after falling ill between fruit.By the fresh fruit after packaging, with light packs in leatheroid fruit case, room temperature 20-25 DEG C, relative humidity is preserve under 80-95% condition.
The Major Diseases causing precocious tangerine to rot is blossom-end rot, anthracnose, and this is also the reason that example laboratory selects precocious tangerine, to observe the prevention effect of statistics to blossom-end rot.Process with antistaling agent prepared by the embodiment of the present invention 1 ~ 5 and corresponding comparative examples 1 ~ 5 respectively, often process fruit 30kg, be treated to blank with clear water, room temperature 20-25 DEG C, relative humidity is preserve under 80-95% condition naturally to rot afterwards and incidence for 30 days.
It is 25.6% that clear water blank preserves rotting rate after 30 days, and the wherein blossom-end rot incidence of disease 18.9%, concrete outcome is as shown in table 2.Result further demonstrate that: 1, polyaminopropyl biguanide is to citrus disease tool broad spectrum activity, reduces orange storing morbidity and rots, and can be used for blossom-end rot control, reduce the incidence of disease of blossom-end rot.2, polyaminopropyl biguanide and anticorrisive agent and bioactivator tool synergistic function, can improve and prevent and treat efficiency to disease.Oranges and tangerines edible quality in embodiment 1 ~ 5 is obviously better than corresponding comparative examples 1 ~ 5, can keep the freshness of fruit better.
Table 2
Rotting rate (%) Blossom-end rot (%)
Clear water is blank 25.56 18.89
Reference examples 1 13.56 5.39
Reference examples 2 9.9 5.21
Reference examples 3 10.12 3.54
Reference examples 4 15.68 6.73
Reference examples 5 13.24 5.41
Embodiment 1 11.11 3.33
Embodiment 2 7.68 2.75
Embodiment 3 5 1.11
Embodiment 4 9.52 3
Embodiment 5 8.9 2.5

Claims (3)

1. for the control of oranges and tangerines blossom-end rot containing the antistaling agent of polyaminopropyl biguanide, its composition and proportioning as follows:
2. described in claim 1 for the preparation method of antistaling agent containing polyaminopropyl biguanide of oranges and tangerines blossom-end rot control, its step is as follows:
(1) take the commercially available polyaminopropyl biguanide of 45g, be placed in the container with agitator, add water stirring 1.5 hours, dissolves, obtain polyaminopropyl biguanide solution; (2) in the container of another belt stirrer, add commercially available citric acid 6g, shitosan 4g, adds suitable quantity of water, stirs 30 points, prepares uniform mixed liquor; (3) step (2) gained mixed liquor and the mixing of step (1) gained polyaminopropyl biguanide solution, add commercially available sorbitanmonolaureate 3g again, commercially available calcium lactate 4g, commercially available propane diols 4g, mixing, moisturizing, to 100g, is stirred 30 minutes, fully emulsified mixing, must containing the antistaling agent of polyaminopropyl biguanide.
3. described in claim 1 for the application of the antistaling agent containing polyaminopropyl biguanide of oranges and tangerines blossom-end rot control after control oranges and tangerines are adopted in blossom-end rot.
CN201410202170.8A 2014-05-13 2014-05-13 A kind of antistaling agent containing polyaminopropyl biguanide for the control of oranges and tangerines blossom-end rot and Synthesis and applications thereof Active CN103976015B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410202170.8A CN103976015B (en) 2014-05-13 2014-05-13 A kind of antistaling agent containing polyaminopropyl biguanide for the control of oranges and tangerines blossom-end rot and Synthesis and applications thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410202170.8A CN103976015B (en) 2014-05-13 2014-05-13 A kind of antistaling agent containing polyaminopropyl biguanide for the control of oranges and tangerines blossom-end rot and Synthesis and applications thereof

Publications (2)

Publication Number Publication Date
CN103976015A CN103976015A (en) 2014-08-13
CN103976015B true CN103976015B (en) 2016-04-06

Family

ID=51268389

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410202170.8A Active CN103976015B (en) 2014-05-13 2014-05-13 A kind of antistaling agent containing polyaminopropyl biguanide for the control of oranges and tangerines blossom-end rot and Synthesis and applications thereof

Country Status (1)

Country Link
CN (1) CN103976015B (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104982516B (en) * 2015-06-15 2019-05-14 珠海真绿色技术有限公司 A kind of the green ecological preservation method and antistaling agent of citrus
CN105494617A (en) * 2015-12-28 2016-04-20 青岛博泰美联化工技术有限公司 Citrus preservative and preparation method thereof
CN106259868A (en) * 2016-07-31 2017-01-04 湘潭大学 A kind of method that sodium diacetate is fresh-keeping to adopting rear orange storing
CN106332891A (en) * 2016-08-26 2017-01-18 安徽久易农业股份有限公司 Weed killer containing fluthiacet-methyl and pendimethalin
CN106635028A (en) * 2016-12-31 2017-05-10 吴小萍 Soil conditioner and preparation method thereof
CN107535588A (en) * 2017-10-31 2018-01-05 中国农业科学院柑桔研究所 The fresh-preserving agent for oranges and tangerines of high-efficiency low-toxicity
CN108208137A (en) * 2017-12-29 2018-06-29 河南健特生物科技集团有限公司 Antimicrobial antistaling agent for fresh-keeping of vegetables and its preparation method and application
CN108419828A (en) * 2018-03-21 2018-08-21 江西农业大学 A kind of citrus comprising loquat-leaf extract adopts rear plant antistaling agent
AT521125A1 (en) * 2018-03-23 2019-10-15 Bcsk Biocid Gmbh Liquid composition

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101019571A (en) * 2007-02-12 2007-08-22 南昌大学 Browning preventing broad spectrum fruit preservative and its prepn process
US20090306154A1 (en) * 2006-07-13 2009-12-10 Symrise Gmbh & Co., Kg Synergistic anti-microbial mixtures of tropolone (derivatives) and selected compounds
CN102461652A (en) * 2010-11-14 2012-05-23 中国科学院华南植物园 Application of polyhaxemethylenguanidine hydrochloride on preventing and controlling orange sour rot and preservative for citrus
CN103461472A (en) * 2013-09-17 2013-12-25 南京通泽农业科技有限公司 Citrus preservative

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090306154A1 (en) * 2006-07-13 2009-12-10 Symrise Gmbh & Co., Kg Synergistic anti-microbial mixtures of tropolone (derivatives) and selected compounds
CN101019571A (en) * 2007-02-12 2007-08-22 南昌大学 Browning preventing broad spectrum fruit preservative and its prepn process
CN102461652A (en) * 2010-11-14 2012-05-23 中国科学院华南植物园 Application of polyhaxemethylenguanidine hydrochloride on preventing and controlling orange sour rot and preservative for citrus
CN103461472A (en) * 2013-09-17 2013-12-25 南京通泽农业科技有限公司 Citrus preservative

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
双胍类消毒防腐剂的应用;李传秋 等;《食品与药品》;20081231;第10卷(第7期);第44-47页 *

Also Published As

Publication number Publication date
CN103976015A (en) 2014-08-13

Similar Documents

Publication Publication Date Title
Martínez-Romero et al. Aloe vera gel coating maintains quality and safety of ready-to-eat pomegranate arils
Mari et al. Non‐conventional methods for the control of post‐harvest pear diseases
Morales et al. Influence of post-harvest technologies applied during cold storage of apples in Penicillium expansum growth and patulin accumulation: A review
CN102458129B (en) Antimicrobial compositions and related methods of use
CN104703926B (en) Stable percarboxylic acids compositions and its purposes
Oro et al. Volatile organic compounds from Wickerhamomyces anomalus, Metschnikowia pulcherrima and Saccharomyces cerevisiae inhibit growth of decay causing fungi and control postharvest diseases of strawberries
Delaquis et al. Survival and growth of Listeria monocytogenes and Escherichia coli O157: H7 in ready-to-eat iceberg lettuce washed in warm chlorinated water
CN101263828B (en) Disinfection antistaling agent containing vinegar liquid for plant and uses thereof
CN106343010A (en) Vegetable preservative containing plant essential oil and application
Martínez-Sánchez et al. Respiration rate response of four baby leaf Brassica species to cutting at harvest and fresh-cut washing
Corbo et al. Fresh-cut fruits preservation: current status and emerging technologies
Heard Microbiology of fresh-cut produce
CN101473991B (en) Compound antistaling agent and fresh-keeping method of glutinous rice cake
Tianli et al. Spoilage by Alicyclobacillus bacteria in juice and beverage products: chemical, physical, and combined control methods
de Oliveira et al. Effects of chitosan from Cunninghamella elegans on virulence of post-harvest pathogenic fungi in table grapes (Vitis labrusca L.)
CN101690514B (en) Preparation method for honey peach fresh-keeping emulsion and application of honey peach fresh-keeping emulsion
CN100496260C (en) Biological preservative for fruits and vegetable, and its preparation method
Samapundo et al. The influence of modified atmospheres and their interaction with water activity on the radial growth and fumonisin B1 production of Fusarium verticillioides and F. proliferatum on corn. Part I: The effect of initial headspace carbon dioxide concentration
CN102018266B (en) All-natural plant composite preservative and preparation method and use method thereof
CN101642126B (en) Compound bactericidal composition containing fludioxonil
CN101156627B (en) Citrus corrosion protection antistaling agent with tea saponin
Palou et al. Evaluation of food additives as alternative or complementary chemicals to conventional fungicides for the control of major postharvest diseases of stone fruit
García-Ruiz et al. Antimicrobial phenolic extracts able to inhibit lactic acid bacteria growth and wine malolactic fermentation
US8859743B2 (en) Controlling microbial contamination in alcoholic fermentation process
Chandra et al. Changes in microbial population and quality of microgreens treated with different sanitizers and packaging films

Legal Events

Date Code Title Description
PB01 Publication
C06 Publication
SE01 Entry into force of request for substantive examination
C10 Entry into substantive examination
GR01 Patent grant
C14 Grant of patent or utility model