CN106501063A - Sample mixing arrangement and its method using uncovered pipe - Google Patents

Sample mixing arrangement and its method using uncovered pipe Download PDF

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Publication number
CN106501063A
CN106501063A CN201510889412.XA CN201510889412A CN106501063A CN 106501063 A CN106501063 A CN 106501063A CN 201510889412 A CN201510889412 A CN 201510889412A CN 106501063 A CN106501063 A CN 106501063A
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CN
China
Prior art keywords
sample
uncovered pipe
pipe
uncovered
mixing arrangement
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Granted
Application number
CN201510889412.XA
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Chinese (zh)
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CN106501063B (en
Inventor
金炳澈
李永珩
郑景和
李民焕
朴婑珍
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Boditech Med Inc
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Boditech Med Inc
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Publication of CN106501063A publication Critical patent/CN106501063A/en
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38Diluting, dispersing or mixing samples

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  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

The present invention relates to one kind utilizes the sample mixing arrangement of uncovered (capless) pipe and its method, the device to include:Clamp portion (110), its support uncovered pipe (10) so that the uncovered pipe is upright and carries out spinning motion;Drive division (120), its produce the driving force for uncovered pipe (10) spinning motion.

Description

Sample mixing arrangement and its method using uncovered pipe
Technical field
The present invention relates to one kind can utilize uncovered (capless) pipe come the devices and methods therefor of mixing sample.
Background technology
Have in clinical immunoassays device a lot of using the blood taking tube for holding the blood that hospital is gathered from patient body The equipment of (blood collection tube).Blood mainly to being contained in blood taking tube separates blood by centrifugation Starch the equipment that tests with blood plasma is only gathered after blood cell.
But, as Check-Out Time is long, and the method for separation detection sample is excessively complicated, be therefore proposed a lot of directly Clinical immunoassays equipment using whole blood.The whole blood of blood taking tube is collected through (about a few minutes) after a while, due to density Big blood cell sinks and the past floating of blood plasma, interval not according to acquisition time and the collection of the sample being contained in inside blood taking tube Same, mistake occurs in the gatherer process of target (target) protein for being used.For solving this problem, when using blood sampling When blood in pipe is diagnosed, artificially mix blood to use.
Blood taking tube takes care of blood in the state of the lid for being stamped quality of rubber materials, when needing to take a blood sample, by by syringe Pin penetrate on lid the collection of realizing blood.From before blood sampling blood sampling tube, as previously discussed, filled using blood mixing Put to mix the blood in blood taking tube.
In FIG, (a), (b), (c), (d) illustrate common blood mixing arrangement, such as shown in (a), (b), on pallet In the state of portion keeps flat blood taking tube, it is rotated in the range of predetermined angular or oscillating movement is mixing blood, or such as Shown in (c), blood taking tube is rotated along the vertical direction, or as shown in (d), with scattered on the pallet being rotated Penetrate shape to fix blood taking tube and realize the mixing of blood.
But, this mixed merga pass for carrying out blood in the state of blood taking tube closes the lid is used for acquisition testing sample Pin be difficult suitable for automated analysis equipment a series of processes for realizing the collection of blood.
Specifically, in automatization's device for analyzing samples during collecting sample should by minute hand being pricked into the lid of blood taking tube in come Collecting sample, now, the syringe needle due to carrying out a sample collection is contaminated, it is necessary to cleaned, therefore, analysis dress Except for during pin is pricked into lid and in addition to the device extracted, also separately possessing for cleaning the device of syringe needle, causing in putting The complex structure of analytical equipment and become large-sized.
For this purpose, when the present inventor realizes the automatization of sample collection procedure, in order to exclude the syringe needle that separately sets and and this Adjoint ancillary equipment and utilize uncovered pipe, so as to develop be conducive to sample analyses collection automatization sample mixing arrangement come The application present invention's.
Prior art literature
No. 4118801 (grant dates of U.S. Patent No.:On October 3rd, 1978)
Content of the invention
(1) technical problem to be solved
The present invention is in order to solve above-mentioned problems of the prior art and to propose, and its object is to provide one kind Be conducive to sample mixing arrangement and its method for the automatic analysis equipment of sample.
(2) technical scheme
Realize that the sample mixing arrangement of the present invention of above-mentioned purpose includes:Clamp portion, its support uncovered pipe so that the nothing Lid pipe is upright and carries out spinning motion;Drive division, its produce the driving force for the uncovered pipe spinning motion.
Preferably, it is characterised in that the drive division can be arranged on the upper end of the uncovered pipe up or down.
It is highly preferred that the drive division includes:Crane, its can be moved up and down;First driving source, its drive described up and down Crane;Fixing head, its can be rotatably set in the crane, and be adjacent to the upper end of the fixation uncovered pipe;Second drives Dynamic source, fixing head described in its rotation driving.
Preferably, it is characterised in that the clamp portion is placed on the top of the uncovered pipe and is freely rotatable.
Preferably, it is characterised in that the drive division is by mutually fetching generation rotation driving with the side of the uncovered pipe Power.
It is highly preferred that the clamp portion includes that multiple sides with the uncovered pipe connect and rotatable driven rolling Wheel, it is highly preferred that the clamp portion at least includes more than one return idler, the return idler be elastically supported and can before After move.
Preferably, it is characterised in that the drive division is by mutually fetching generation rotation driving with the lower end of the uncovered pipe Power, more preferably, it is characterised in that the clamp portion flexibly supports the upper end of uncovered pipe.
Then, it is characterised in that the sample mixed method of the utilization uncovered pipe of the present invention is the uncovered by erectility The spinning motion of pipe carrys out mixing sample.
Preferably, it is characterised in that produce rotary driving force by driving source, the driving source is by the nothing The upper end of lid pipe carries out moving up and down to be adjacent in the upper end of the uncovered pipe, more preferably, it is characterised in that the driving source Rotation is carried out during contacting with the uncovered pipe.
(3) beneficial effect
It is will not have the uncovered pipe for covering uprightly come by spinning motion according to sample mixing arrangement of the invention and its method The mixing of sample is realized, so as to make in the sample collection operation that carries out after having mixed sample in the automatic analysis equipment of sample Use disposable sampling instrument, rather than use syringe needle, particularly without use for collecting sample injection needle, therefore, Can be simplified certainly for operating the structure of injection needle and cleaning equipment adjoint with this etc. by excluding in automatic analysis equipment The apparatus structure of dynamicization equipment.
Description of the drawings
(a), (b), (c) of Fig. 1, (d) are the photos for illustrating normal blood mixing arrangement.
Fig. 2 is the structure chart of the sample mixing arrangement of the present invention.
(a), (b), (c) of Fig. 3, (d) are the figures for the operation example of the sample mixing arrangement of the present invention is described.
(a), (b) of Fig. 4 be illustrate according to the present invention sample mixing arrangement sample mixing before and after process photo.
Fig. 5 is the structure chart of the sample mixing arrangement of the preferred embodiments of the present invention.
(a), (b) of Fig. 6 is the structure chart of the sample mixing arrangement of another embodiment of the present invention.
Fig. 7 is the structure chart of the sample mixing arrangement of another embodiment of the present invention.
Description of the drawings labelling
100:Uncovered pipe 110:Clamp portion
120、220、310、410:Drive division 330:Jig plate
Specific embodiment
Ad hoc structure and the functional description for proposing in embodiments of the present invention is simply to illustrate that according to the general of the present invention The embodiment of thought and illustrate, therefore, the embodiment according to idea of the invention can be implemented in a variety of forms.And, must not It is construed to be defined in the embodiment that illustrate in this specification, it is understood that be all in the thought and technical scope for include the present invention Change thing, equivalent and substitute.
In addition, in the present invention, the term such as first and/or second can be used to various assemblies are described, but the component is not It is defined in the term.The term is intended merely to a component is distinguished with other assemblies and used, and for example, is not taking off In the rights protection scope of idea of the invention, first assembly can be named as the second component, and similarly, the second component can be named For first assembly.
When certain component is mentioned with another component " connection " or " engagement ", it can be understood as be directly connected to another component Or engagement, but it is also possible to it is interpreted as the other components of middle presence.Conversely, " direct with another component when certain component is mentioned When connection " or " directly contact ", it is thus understood that centre does not have other assemblies.For illustrate the relation between component other Statement, i.e. "~between " and " directly~between " or " with~adjacent " and " with~direct neighbor " etc. state also Ying Yixiang Same mode is explained.
The term that used in this manual simply to illustrate that specific embodiment and use, do not limit It is intended to.Implication unless clearly there are other implications within a context in the statement of odd number, otherwise comprising plural number.In this specification The term of " including " or " with " etc. is to specify implemented feature, numeral, step, operation, component, part or its group The presence of conjunction, it is not excluded that one or more other features, numeral, step, operation, component, part or its group The presence of conjunction, or additional probability.
The present invention is described in detail with reference to the accompanying drawings.
With reference to Fig. 2, the sample mixing arrangement of the present invention includes:Clamp portion 110, its support uncovered pipe 10 so that uncovered pipe 10 uprightly and carry out spinning motion;Drive division 120, its produce the driving force for 10 spinning motion of uncovered pipe.
The pipe for being used in the present invention is the uncovered pipe 10 for not covering other lid in upper end opening portion, now, Sample can be the liquid samples such as whole blood sample, furthermore, it is possible to be the mixture of liquid and solid-state.
Clamp portion 110 includes:Rotating disk 113, its upper side are formed with placing trough 111, and lower end possesses the rotation of axle insertion Rotating shaft 112;Bearing 114, its support rotary shaft 112 so that rotary shaft is freely rotatable.
Placing trough 111 can be the groove with the bending that curved surface is identical or radius of curvature is somewhat larger of 10 lower end of pipe, so that A part for 10 lower end of pipe is inserted into into, now, can be provided with the guide member for supporting 10 sidepiece of uncovered pipe so that uncovered Pipe 10 keeps standing state.
Further, since placing trough has sufficient depth, uncovered pipe also can be in the help without other guide member Lower self-support (self-standing) and be located in placing trough.
Drive division 120 can be arranged on the upper end of uncovered pipe 10 up or down, specifically, it may include:Fixing head 121, its Fix and support the upper end of uncovered pipe 10;Driving source 122, its rotate and drive fixing head 121.
In Fig. 2, it is provided with for making the other driving source and guide its movement side that drive division 120 lifted along the vertical direction To guide, this is illustrated with reference to the accompanying drawings again.
Driving source 122 can be known various electro-motors, and, can increase between fixing head 121 and driving source 122 If for the driving force transfering device for transmitting driving force, such as gear or transmission belt etc..
The fixing hole 121a of the upper end that can accommodate uncovered pipe 10 is formed with the lower surface of fixing head 121, it is preferable that formed There is the guide surface 121b for obliquely expanding and being formed from fixing hole 121a, so as to the upper end of uncovered pipe 10 can be along fixing head 121 guide surface 121b is properly placed in fixing hole 121a.
Preferably, friction pad 123, the contact with uncovered pipe 10 of friction pad 123 can be provided with the bottom surface of fixing hole 121a Face has big frictional force, therefore, it is possible to prevent uncovered pipe 10 from sliding during the rotary motion of fixing head 121 (slip), and the rotary motion of fixing head 121 can be correctly delivered to uncovered pipe 10.
Each rotary shaft of clamp portion 110 and drive division 120 is arranged on same axle C, and uncovered pipe 10 is carried out on the axle C Spinning motion, so that realize the mixing of sample.
(a), (b), (c) of Fig. 3, (d) are the figures for the operation example of the sample mixing arrangement of the present invention is described.
With reference to (a) of Fig. 3, the top that the uncovered pipe 10 for being contained with sample is placed on clamp portion 110, then drive division 120 are declined and are adjacent to so as to fixed upper end with uncovered pipe 10.
Preferably, with low speed rotation while drive division 120 declines such that it is able to fixed uncovered pipe 10.
When uncovered pipe 10 is placed into the top of clamp portion 110, it is possible to not quite identical with the direction of the axis of rotation, at this In invention, as drive division 120 is to decline while low speed rotation, the upper end of uncovered pipe 10 is contacted with the guide surface 121b for inclining And along rotation drive division 120 rotary shaft naturally, be precisely placed in fixing hole 122, so as to realize fixing.
In addition, the rotary speed of the drive division 120 occurred during the decline of drive division 120 can be in the sample than uncovered pipe 10 Suitably determine in the low scope of the rotary speed that rotates during mixing.
Then, as shown in (b), (c) of Fig. 3, by the rotary motion of drive division 120, uncovered pipe 10 enters to same direction Row rotary motion, so as to mixing sample, now, the rotary motion of uncovered pipe 10 can be that the rotation carried out to a direction is transported Dynamic, or the time every regulation, to repeating rotary motion clockwise or counterclockwise.
As shown in (d) of Fig. 3, after sample has been mixed, drive division 120 rises and returns to original position, so as to carry out nothing Sample collection and analysis work in lid pipe 10.
(a), (b) of Fig. 4 is to illustrate to realize mixing by the blood in each pipe of rotation (rotation) under prescribed conditions The photo of state in front and back, (a) is photo that before mixing pipe is shot, is (b) by the rotation speed of Guan Yiyue 6000RPM The photo shot after carrying out rotating 35 seconds by degree.
In (b) of Fig. 4, the maximum liquid of the blood produced because of centrifugal force when reference ' d ' represents pipe rotation (rotation) Face difference in height, can be sufficiently mixed in rotation (rotation) velocity interval of blood, liquid level in general pipe diameter scope (number cm) Difference in height about within 1cm, it can thus be appreciated that its difference in height is not very big, even if mixing arrangement therefore of the invention is by rotation Uncovered pipe carrys out mixing sample, and in mixed process, sample will not also overflow and can realize mixing.
Fig. 5 is the structure chart of the sample mixing arrangement of the preferred embodiments of the present invention, for embodiments illustrated above Identical structure, using identical reference, and the repetitive description thereof will be omitted content.
With reference to Fig. 5, the sample mixing arrangement according to the present embodiment includes:Clamp portion 110, can make uncovered pipe 10 upright simultaneously Carry out spinning motion;Drive division 220, its produce the driving force for 10 spinning motion of uncovered pipe.
Drive division 220 includes:Crane 221, its can be moved up and down;First driving source 222a, 222b, its drive liter up and down Drop frame 221;Fixing head 223, its can be rotatably set in crane 221, and be adjacent to the upper end of fixed uncovered pipe 10;Second drives Dynamic source 225a, 225b, its are connected with fixing head 223 so as to providing rotary driving force using conveyer belt 224 as medium.
Crane 221 can be arranged up or down, for example, can pass through the shell for being fixed on the outward appearance for constituting mixing arrangement The guide of (not shown) is realizing the guiding of above-below direction.Crane 221 can be provided with fixing head 223 and the second driving source 225a、225b.
First driving source 222a, 222b operates crane 221 along the vertical direction, in the present embodiment, the first driving source bag Include the first drive motor 222a and the height of crane 221 is adjusted by first drive motor 222a come forward or reverse Ball screw 222b.
Fixing head 223 can be rotatably set in the fixing head on crane 221, moving up and down together with crane 221 223, which is as one man moved up and down with the axis of rotation C directions of uncovered pipe 10.
In addition, engagement pad 223a that can be provided with being adjacent to the upper end of uncovered pipe 10 in the lower end of fixing head 223.
Second driving source 225a, 225b provides the rotary driving force of fixing head 223, including:Second drive motor 225a, its It is arranged on crane 221;Conveyer belt 224, its transmit the rotary driving force of the second drive motor 225a to fixing head 223.
In the present embodiment, the second drive motor 225a is provided with driving wheel 225b, and driving wheel 225b passes through conveyer belt 224 with to fixing head 223 upper end extend driven shaft 223b be connected, now, driving wheel 225b with diameter greater than driven shaft The diameter of 223b such that it is able to obtain big speed increasing ratio.
The sample mixing arrangement of the present embodiment for so constituting can realize fixing head 223 by the first drive motor 222a Upper-lower position operation, and control the rotary speed of fixing head 223 by the second drive motor 225a such that it is able to mix nothing Sample in lid pipe 10.
In addition, the first drive motor 222a and the second drive motor 225a realize control by unshowned controller, This operation can be operated in a manual manner in person by user, or in the sample analysis apparatus of automatization be used as aggregate sample This single-bit module, and be automatically obtained by a series of control instruction that programmed.
In above embodiment, the driving source exemplified with the spinning motion for uncovered pipe is configured in uncovered pipe End, but this is not limited to, side or lower end in uncovered pipe is can also be configured, is described in detail below and this is embodied as Example.
(a), (b) of Fig. 6 is the structure chart of the sample mixing arrangement of another embodiment of the present invention.
With reference to Fig. 6, the sample mixing arrangement of another embodiment of the present invention includes:Drive division 310, itself and uncovered pipe 10 Side mutually fetches and realizes rotation driving;Clamp portion, its are connected with the side of uncovered pipe 10 and are freely rotatable.
Drive division 310 includes:Drive motor 312, its are fixedly installed on fixed mount 311;Driving rolls 314, its pass through to pass Send and be connected with drive shaft 312a of drive motor 312 with 313, and mutually fetch with uncovered pipe 10 and realize rotary motion.
Clamp portion may include positioned at the opposition side of driving rolls 314 and be connected with the side of uncovered pipe 10 and be freely rotatable Multiple return idlers 321,322.
In the present embodiment, return idler 321,322 is made up of the first return idler 321 and the second return idler 322, excellent Selection of land, the first return idler 321 and the second return idler 322 are symmetrically configured on the basis of vertical VP, now, vertically Face VP refers to the rotary shaft of driving rolls 314 and the plane of the C that walks around certainly.
Preferably, the rotary shaft of at least one of two return idlers 321,322 is movably disposed, and by elastomer 323 resilient support.
In the present embodiment, the first return idler 321 abreast can be moved forward and backward with vertical VP, and now, first is driven Rotary shaft 321a of roller 321 is flexibly supported by elastomer 323.
Therefore, when uncovered pipe 10 is inserted in the position relative with the first return idler 321 to horizontal direction, elastomer 323 while compressed, and the first return idler 321 can be retreated, so as to realize the insertion of uncovered pipe 10, afterwards, when uncovered pipe 10 When being located on axis of rotation C, the first return idler 321 is advanced again by the restoring force of elastomer 323, so as to be adjacent in uncovered pipe 10 side.
The sample mixing arrangement for so constituting is by the driving rolls 314 that rotates together with multiple return idlers 321,322 Rotary motion realizing the spinning motion of uncovered pipe 10 such that it is able to realize the mixing of sample.
In addition, in the present embodiment, uncovered pipe 10 is located at certainly by driving rolls 314 and multiple return idlers 321,322 So as to mixing sample in rotating shaft C, it is preferable that the lower end and rotatable jig plate for being installed on uncovered pipe 10 can be set up 330, this jig plate 330 can be identical with the clamp portion 110 in embodiments illustrated above (with reference to Fig. 2).
Fig. 7 is the structure chart of the sample mixing arrangement of another embodiment of the present invention.
With reference to Fig. 7, the sample mixing arrangement of the present embodiment includes:Drive division 410,10 portion disposed thereon of uncovered pipe and is carried out Spinning motion;Resilient support portion 420, its flexibly support the upper end of uncovered pipe 10.
Drive division 410 may include:Drive motor 411;First rotary body 412, which passes through drive motor 411 carries out rotation fortune Dynamic;Second rotary body 413, which is external with the first rotary body 412 and is rotated.
In order to obtain big speed increasing ratio, the diameter of the first rotary body 412 can be more than the diameter of the second rotary body 413, and first Rotary body and the second rotary body can be the gears of mutual tooth connection, but be not limited to this, can also pass through transmission belt connect from And transmit rotary driving force.
Uncovered pipe 10 is vertically located at the top of the second rotary body 413, and the upper end of uncovered pipe 10 is by the portion that flexibly supports 420 To support.
Resilient support portion 420 supports the upper end of uncovered pipe 10, for example, it may be flat spring.
Present invention mentioned above is not limited to embodiments illustrated above and accompanying drawing, in the technology without departing from the present invention In the range of thought, general technical staff of the technical field of the invention can carry out various displacements, deformation and change.

Claims (12)

1. a kind of sample mixing arrangement, which includes:
Clamp portion, its support uncovered pipe so that the uncovered pipe is upright and carries out spinning motion;
Drive division, its produce the driving force for the uncovered pipe spinning motion.
2. sample mixing arrangement according to claim 1, it is characterised in that the drive division can be arranged on up or down The upper end of the uncovered pipe.
3. sample mixing arrangement according to claim 2, the drive division include:
Crane, its can be moved up and down;
First driving source, its drive the crane up and down;
Fixing head, its can be rotatably set in the crane, and be adjacent to the upper end of the fixation uncovered pipe;
Second driving source, fixing head described in its rotation driving.
4. sample mixing arrangement according to claim 1, it is characterised in that the clamp portion is placed on the uncovered pipe Top and it is freely rotatable.
5. sample mixing arrangement according to claim 1, it is characterised in that the drive division by with the uncovered pipe Side mutually fetches generation rotary driving force.
6. sample mixing arrangement according to claim 5, the clamp portion include multiple side phases with the uncovered pipe Connect and rotatable return idler.
7. sample mixing arrangement according to claim 6, the clamp portion at least include more than one return idler, institute State return idler to be elastically supported and can move forward and backward.
8. sample mixing arrangement according to claim 1, it is characterised in that the drive division by with the uncovered pipe Lower end mutually fetches generation rotary driving force.
9. sample mixing arrangement according to claim 8, it is characterised in that the clamp portion resilient support uncovered pipe upper End.
10. a kind of sample mixed method, which is the sample mixed method using uncovered pipe, it is characterised in that by erectility The spinning motion of uncovered pipe carry out mixing sample.
11. sample mixed methods according to claim 10, it is characterised in that rotation driving is produced by driving source Power, the driving source is by carrying out moving up and down to be adjacent in the upper end of the uncovered pipe in the upper end of the uncovered pipe.
12. sample mixed methods according to claim 11, it is characterised in that the driving source is being connect with the uncovered pipe Rotation is carried out during touching.
CN201510889412.XA 2015-09-03 2015-12-07 Sample mixing device using uncovered tube and method thereof Active CN106501063B (en)

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KR1020150124610A KR101728711B1 (en) 2015-09-03 2015-09-03 Mixing apparatus and method utilizing a capless tube
KR10-2015-0124610 2015-09-03

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CN106501063B CN106501063B (en) 2021-11-30

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CN114504979A (en) * 2022-01-13 2022-05-17 许昌市传染病医院 Medical treatment detects with vibration mixing device

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US4845025A (en) * 1987-11-10 1989-07-04 Coulter Corporation Biological sample mixing apparatus and method
US6235537B1 (en) * 1996-06-20 2001-05-22 Cytek Corporation Methods for washing cells
JP2005321306A (en) * 2004-05-10 2005-11-17 Tosoh Corp Sample agitating apparatus
CN1865899A (en) * 2006-05-26 2006-11-22 段铭 Sample absorbing device capable of automatically blending blood sample
DE102009007822A1 (en) * 2009-02-07 2010-08-19 PRO DESIGN Gesellschaft für Produktentwicklung mbH Shaking or vibrating device for producing flow disturbance in sample in laboratory during e.g. mixing of liquid, has holder, where container rotates with holder with respect to center axis that rotates about vertical axis on conical surface
CN102033007A (en) * 2009-09-30 2011-04-27 希森美康株式会社 Blood sample processing apparatus and blood sample processing method
CN102151510A (en) * 2011-02-14 2011-08-17 四川迈克生物科技股份有限公司 Mixing device
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CN104568564A (en) * 2015-02-13 2015-04-29 重庆天海医疗设备有限公司 Intelligent stirring and uniformly mixing device for blood collection tubes

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KR101728711B1 (en) 2017-04-21
KR20170028467A (en) 2017-03-14

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