CN106495335A - A kind of method of rhodium ion in utilization HCl treatment Pichia sp. absorption waste water - Google Patents
A kind of method of rhodium ion in utilization HCl treatment Pichia sp. absorption waste water Download PDFInfo
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- CN106495335A CN106495335A CN201611025002.1A CN201611025002A CN106495335A CN 106495335 A CN106495335 A CN 106495335A CN 201611025002 A CN201611025002 A CN 201611025002A CN 106495335 A CN106495335 A CN 106495335A
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- pichia
- waste water
- thalline
- rhodium ion
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
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Abstract
The invention discloses a kind of method that utilization HCl treatment Pichia sp. adsorbs rhodium ion in waste water, belongs to sewage treatment area.The method of the present invention improves the absorption to rhodium ion in waste water by carrying out HCl treatment to Pichia sp. thalline.The present invention is with 0.1～0, the thalline that the HCl of 3mol/L is processed, and during thalline is added to the sewage containing rhodium ion, 5～9g/L of cell concentration is adjusted, pH 9～11 is adjusted, in 30～40 DEG C, 200～220rpm processes 120～240min, in sewage 92.3% rhodium ion can be adsorbed, and easy to operate, low cost, with important industrial application value.
Description
Technical field
The present invention relates to a kind of method that utilization HCl treatment Pichia sp. adsorbs rhodium ion in waste water, belongs to sewage disposal
Field.
Background technology
For preventing the pollution of the environment, industrial wastewater is needed through processing, and can just be discharged after reaching standard.Process containing a huge sum of money
The sewage water treatment method of category has a lot, such as chemical precipitation method, ion-exchange-resin process, active carbon adsorption, electrolysis and film point
From method etc., but these methods because cumbersome and have secondary pollution, all not ideal enough.
On the other hand, the sewage disposal containing rare precious metal is in addition to the factor that need to consider Environmental security, resource reclaim
It is the one side that can not be ignored.Traditional rare precious metal secondary resource is reclaimed and mainly adopts physical-chemical process.Wherein solid waste
The pyrometallurgy technology that is most commonly used that is reclaimed, and its ultimate principle is nonmetallic substance to be peeled off using metallurgical furnace high temperature heating, makes dilute
Noble metal is melted in other Metal Melting materials or fused salt, then is separated.Pyrometallurgy has process is simple and the response rate
High the features such as, have the disadvantage that energy consumption is big, easily produce secondary pollution, and the rare precious metal purity that general pyrogenic process process is reclaimed is not high.
And the method for rare precious metal is reclaimed from the liquid secondary resource such as waste water, waste liquid then than wide, including chemical precipitation method, electrolysis
Method, ion exchange, membrane technology partition method and resin adsorption method etc..Although these methods can reach certain effect,
When reclaiming rare precious metal, cumbersome, investment is high with operating cost, and can only typically reclaim dilute in high concentration metal waste liquid
Noble metal, produces substantial amounts of secondary waste in removal process, it is therefore desirable to the secondary money of rare precious metal of a kind of low cost and environmental protection
Source circulation utilization method.
Content of the invention
In order to overcome above the deficiencies in the prior art, the invention provides one kind improves which using HCl treatment Pichia sp.
The new method of rhodium ion ability in absorption waste water, by adjusting Pichia sp. in waste liquor PH, adsorption temp, cell concentration, absorption
The adsorption conditionses such as time, microbial activity, reach the purpose of the rhodium ion in absorption waste water.
The method that first purpose of the present invention is to provide rhodium ion in a kind of absorption waste water, methods described is with hydrochloric acid pair
Pichia sp. is processed, then carries out rhodium during Pichia sp. is added to waste water with the final concentration of 5～9g dry weights thalline/L waste water
The absorption of ion.
In one embodiment of the invention, described Pichia sp. is carried out processing be by thalline add to 0.1～
In the hydrochloric acid of 0.3mol/L, wash 3～4 times.
In one embodiment of the invention, the pH that methods described adjusts waste water is 9-11.
In one embodiment of the invention, the pH that methods described adjusts waste water is 11.
In one embodiment of the invention, the bacterium of the Pichia sp. is dense for 5g/L.
In one embodiment of the invention, the Pichia sp. passes through inactivation to waste water in addition.
In one embodiment of the invention, the time of the absorption is 120～240min.
In one embodiment of the invention, the temperature of the absorption is 30～40 DEG C.
In one embodiment of the invention, adsorption process is also stirred.
In one embodiment of the invention, the Pichia sp. is enriched with through thalline, and the enrichment is by complete red ferment
Mother is seeded in seed culture medium, in 30 DEG C, the 16～30h of culture of 200～220rpm, then be forwarded in fermentation medium and train
Support.
In one embodiment of the invention, the switching is to be inoculated with seed liquor with 10% volume ratio.
In one embodiment of the invention, the seed culture medium is YEPD fluid mediums；The fermentation culture
Base contains glycerol 20mL, H per L3PO420mL, K2SO41g, (NH4)2SO45g, CaSO40.1g, MgSO41g, PTM110mL.
The present invention also provides application of the methods described in sewage disposal.
Beneficial effects of the present invention：The invention provides a kind of HCl pretreatment Pichia sp. is inhaled to rhodium ion with improving which
The method of attached effect, through the Pichia sp. after method of the present invention process, the lifting of rhodium ion absorbability is obvious, from 20%
Improve to 92.3%；And the microbial activity adsorption effect after processing has not significant impact, adsorption rate can reach more than 90%,
And easy to operate, low cost of the invention, with important industrial application value.
Description of the drawings
Fig. 1 is impact of the pretreatment mode to adsorption rate；
Fig. 2 is the curve linear relationship for adsorbing pH and adsorption rate；
Fig. 3 is impact of the microbial activity to adsorption rate；
Fig. 4 is impact of the adsorption time to adsorption rate；
Fig. 5 is impact of the cell concentration to adsorption rate and adsorbance；
Fig. 6 is the relation curve of adsorption temp and absorption；
Specific embodiment
Slant medium (g/L)：YPD solid mediums：Peptone 20, yeast extract 10, glucose 20, agar 15.
Seed culture medium：YEPD fluid mediums (g/L)：Peptone 20, yeast extract 10, glucose 20.
5L fermentation tank culture mediums (g/L)：Glycerol 20mL/L, H3PO420mL/L, K2SO41g, (NH4)2SO45g,
CaSO40.1g, MgSO41g, PTM110mL/L, pH 6.0.
Cell concentration is measured：Absorbance (the OD of fermentation liquid is detected under 600nm600), according to curve DCW=0.25
×OD600, obtain dry cell weight.
The measure of thalline moisture content：The thalline for gathering is dried at 60 DEG C, in drying course, its weight is constantly measured,
Until its weight no longer changes, this weight is calculated as dry weight；Moisture content presses formula calculating：Moisture content=[(one dry weight of weight in wet base)/wet
Weight] × 100%.
The measure of rhodium content：Using inductive coupling plasma emission spectrograph (ICP-OES), assay method sees reference text
Offer《The different analysis methods of rhodium content in electroplate liquid》(2006 open).
Adsorbance and the calculating of adsorption rate：The initial concentration for determining rhodium ion in waste water is initial concentration (mg L-1), absorption
In waste water afterwards, rhodium ion concentration is final concentration (mg L-1), absorbent concentration be Pichia sp. dry cell weight (g)/contain rhodium ion
Wastewater volume (L), according to equation below calculate：
Adsorbance=(initial concentration-final concentration)/absorbent concentration
Adsorption rate=[(initial concentration-final concentration)]/final concentration × 100%
Embodiment 1
In the triangular flask for choosing that single bacterium colony is inoculated into the 500mL containing 50mL seed culture mediums from YPD washers, in 30
DEG C, 220r min-1Culture 24h as fermentor cultivation seed liquor.By the seed liquor with the inoculum concentration of 10% (volume ratio)
It is inoculated into 16～36h of culture in the fermentation tank of the 5L containing 1.2L fermentation medium.3～10min is centrifuged in 7000rpm, bacterium is collected
Body, is washed with deionized thalline 2～3 times.
Embodiment 2
The Pichia sp. of 0.5g (dry weight) is washed 3～4 times with the HCl of 0.1mol/L, 3000～5000rpm is centrifuged, received
Collection thalline.With deionized water as control, using the washing of above-mentioned identical method, thalline is collected by centrifugation.
The rhodium ion solution of 100mL 50mg/L is prepared in conical flask, the thalline that said method is collected is added separately to
In conical flask, clog bottleneck with tampon, in 30 DEG C, 200rpm processes 60min, calculates adsorption rate, as a result as shown in figure 1, without
The Pichia sp. rhodium ion adsorption rate of HCl process is 20.0%, through the Pichia sp. rhodium ion adsorption rate of HCl process is
66.83%.
Embodiment 3
The Pichia sp. of 0.5g (dry weight) is washed 3～4 times with the HCl of 0.1mol/L, 3000～5000rpm is centrifuged, received
Collection thalline.
The rhodium ion solution of 100mL 50mg/L is prepared in conical flask, pH=1～11 is adjusted with NaOH and HCl, will be upper
State the thalline after method is processed to add to conical flask, in 30 DEG C, 200rpm processes 120min, calculates adsorption rate.As a result such as Fig. 2
Shown, when pH is 7～11, when adsorption rate is 9～11 more than 84.3%, pH, adsorption rate is 90.2～92.3%.
Embodiment 4
The Pichia sp. of 0.5g (dry weight) is washed 3～4 times with the HCl of 0.1mol/L, 3000～5000rpm is centrifuged, received
Collection thalline, the thalline inactivated after washing as stated above and in 121 DEG C of high pressure (0.1MPa) for compareing, at identical conditions
Centrifugation, collects thalline.
The rhodium ion solution of 100mL 50mg/L is prepared in conical flask, the thalline after said method is processed is added to cone
In shape bottle, in 30 DEG C, 200rpm processes 240min, and in 5min, 10min, 30min, 60min, 120min, 180min,
240min determines rhodium ion concentration.As a result as shown in figure 3, the adsorption rate of mycelidium and the absorption rhodium ion of the thalline after inactivation is equal
Higher than 37.2%, process the thalline adsorption rate after 60～240min and be about 90%, and have no and be clearly distinguished from.
Embodiment 5
The Pichia sp. of 0.5g (dry weight) is washed 3～4 times with the HCl of 0.1mol/L, 3000～5000rpm is centrifuged, received
Collection thalline.
The rhodium ion solution of 100mL 50mg/L is prepared in conical flask, the thalline after said method is processed is added to cone
In shape bottle, in 25～30 DEG C, 200rpm processes 240min, respectively at 5min, 30min, 60min, 90min, 120min,
180min, 240min determine rhodium ion concentration and calculate adsorption rate.As a result as shown in figure 4, adsorption time is 120min adsorption rates
Highest, up to 92.3%, the adsorption rate of adsorption time 180min and 240min is respectively 91.2% and 90.6%.
Embodiment 6
The Pichia sp. of 0.5g (dry weight) is washed 3～4 times with the HCl of 0.1mol/L, 3000～5000rpm is centrifuged, received
Collection thalline；The thalline of 0.1g (dry weight)～0.9g (dry weight) is processed as stated above with the HCl of 0.1mol/L respectively.
The thalline point that the rhodium ion solution of some bottles of 100mL 50mg/L is prepared in conical flask, after said method is processed
In not adding to conical flask, in 25～30 DEG C, 200rpm processes 120min, determines rhodium ion concentration and calculates adsorption rate.As a result
As shown in figure 5, adsorption rate is up to more than 92.1% when cell concentration is 5g/L～9g/L.
Embodiment 7
The Pichia sp. of 0.5g (dry weight) is washed 3～4 times with the HCl of 0.1mol/L, 3000～5000rpm is centrifuged, received
Collection thalline.
The rhodium ion solution of 100mL 50mg/L is prepared in conical flask, the thalline after said method is processed is added to cone
In shape bottle, (gradient is set per 5 DEG C) respectively at 15～45 DEG C, 200rpm processes 120min, determines rhodium ion concentration and counts
Calculate adsorption rate.As a result as shown in fig. 6, adsorption effect is ideal when temperature is 30～40 DEG C, about more than 90%；Adsorption temp is
Adsorption rate highest when 30 DEG C, up to 91.9%.
Embodiment 8
The Pichia sp. of 0.5～0.9g (dry weight) is washed 3～4 times with the HCl of 0.1～0.3mol/L, 3000～
5000rpm is centrifuged, collects thalline.
The rhodium ion solution of 100mL 50mg/L is prepared in conical flask, the thalline after said method is processed is added to cone
In shape bottle, pH9～11 are adjusted, in 30～40 DEG C, 200～220rpm processes 120～240min, determine rhodium ion concentration and calculate
Adsorption rate.As a result show, the thalline after HCl process is reached as high as to the adsorption rate of rhodium ion under above-mentioned adsorption conditionses
92.3%.
Although the present invention is disclosed as above with preferred embodiment, which is not limited to the present invention, any is familiar with this skill
The people of art, without departing from the spirit and scope of the present invention, can do various changes and modification, therefore protection model of the invention
Enclosing should be by being defined that claims are defined.
Claims (10)
1. a kind of absorption waste water in rhodium ion method, it is characterised in that methods described be with hydrochloric acid to Pichia sp. at
Reason, then the absorption for carrying out rhodium ion with the final concentration of 5～9g thalline/L waste water during Pichia sp. is added to waste water.
2. method according to claim 1, it is characterised in that described Pichia sp. is carried out processing is by Pichia sp.
Thalline is washed 3～4 times in the hydrochloric acid of 0.1～0.3mol/L.
3. method according to claim 1 and 2, it is characterised in that it is 9-11 that methods described adjusts the pH of waste water.
4. method according to claim 3, it is characterised in that the bacterium of Pichia sp. is dense for 5g/L.
5. according to 1,2,4 arbitrary described method of claim, it is characterised in that the time of absorption is 120～240min.
6. method according to claim 5, it is characterised in that the temperature of absorption is 30～40 DEG C.
7. according to 1,2,4,6 arbitrary described method of claim, it is characterised in that adsorption process is also stirred.
8. method according to claim 7, it is characterised in that Pichia sp. is adding to waste water through inactivation.
9. method according to claim 8, it is characterised in that Pichia sp. is enriched with through thalline, the enrichment are to finish
Red yeast-inoculated into seed culture medium, in 30 DEG C, the 16～30h of culture of 200～220rpm, then be forwarded in fermentation medium
Enrichment culture.
10. claim 1-4, application of 6, the 8 arbitrary methods describeds in sewage disposal.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107381830A (en) * | 2017-07-19 | 2017-11-24 | 宜兴市科兴合金材料有限公司 | The collection method of rhodium ion in a kind of waste water |
| CN107879485A (en) * | 2017-12-13 | 2018-04-06 | 合肥郑国生物科技有限公司 | A kind of biological adsorption recovery method of heavy metals in industrial wastewater |
| CN108031445A (en) * | 2017-12-13 | 2018-05-15 | 合肥郑国生物科技有限公司 | A kind of preparation method and application method of the Pichia kudriavezii adsorbent of quick adsorption heavy metal |
| CN108244460A (en) * | 2018-01-31 | 2018-07-06 | 淄博职业学院 | It is a kind of to improve method of the saccharomyces cerevisiae to Metal Ions in Foodstuffs adsorption capacity |
| CN114669281A (en) * | 2022-04-02 | 2022-06-28 | 中南大学 | PEI modified yeast residue-based platinum-palladium-rhodium adsorption material, preparation method and industrial wastewater treatment method |
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| CN1113890A (en) * | 1992-12-09 | 1995-12-27 | 泰克尼翁研究发展基金有限公司 | Process for the removal of species containing metallicions from effluents |
| CN102557271A (en) * | 2010-12-17 | 2012-07-11 | 上海市长宁区少年科技指导站 | Method for treating heavy-metal-polluted water through waste yeast |
| WO2015121798A1 (en) * | 2014-02-11 | 2015-08-20 | Bortoli Elio Fabio | Biocatalytic composition for treatment of substrates |
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2016
- 2016-11-22 CN CN201611025002.1A patent/CN106495335B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1113890A (en) * | 1992-12-09 | 1995-12-27 | 泰克尼翁研究发展基金有限公司 | Process for the removal of species containing metallicions from effluents |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107381830A (en) * | 2017-07-19 | 2017-11-24 | 宜兴市科兴合金材料有限公司 | The collection method of rhodium ion in a kind of waste water |
| CN107879485A (en) * | 2017-12-13 | 2018-04-06 | 合肥郑国生物科技有限公司 | A kind of biological adsorption recovery method of heavy metals in industrial wastewater |
| CN108031445A (en) * | 2017-12-13 | 2018-05-15 | 合肥郑国生物科技有限公司 | A kind of preparation method and application method of the Pichia kudriavezii adsorbent of quick adsorption heavy metal |
| CN108244460A (en) * | 2018-01-31 | 2018-07-06 | 淄博职业学院 | It is a kind of to improve method of the saccharomyces cerevisiae to Metal Ions in Foodstuffs adsorption capacity |
| CN114669281A (en) * | 2022-04-02 | 2022-06-28 | 中南大学 | PEI modified yeast residue-based platinum-palladium-rhodium adsorption material, preparation method and industrial wastewater treatment method |
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