CN106495335B - A method of utilizing rhodium ion in HCl treatment Pichia pastoris absorption waste water - Google Patents
A method of utilizing rhodium ion in HCl treatment Pichia pastoris absorption waste water Download PDFInfo
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- CN106495335B CN106495335B CN201611025002.1A CN201611025002A CN106495335B CN 106495335 B CN106495335 B CN 106495335B CN 201611025002 A CN201611025002 A CN 201611025002A CN 106495335 B CN106495335 B CN 106495335B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
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Abstract
The invention discloses a kind of methods using rhodium ion in HCl treatment Pichia pastoris absorption waste water, belong to sewage treatment field.Method of the invention improves the absorption to rhodium ion in waste water by carrying out HCl treatment to Pichia pastoris thallus.The present invention is with 0.1~0, the thallus of the HCl processing of 3mol/L, and thallus is added into the sewage containing rhodium ion, 5~9g/L of cell concentration is adjusted, pH 9~11 is adjusted, in 30~40 DEG C, 200~220rpm handles 120~240min, in sewage 92.3% rhodium ion can be adsorbed, and easy to operate, at low cost, there is important industrial application value.
Description
Technical field
The present invention relates to a kind of methods using rhodium ion in HCl treatment Pichia pastoris absorption waste water, belong to sewage treatment
Field.
Background technique
To prevent the pollution of the environment, industrial wastewater is needed by processing, and can just be discharged after reaching standard.Processing is containing a huge sum of money
The sewage water treatment method of category has very much, such as chemical precipitation method, ion-exchange-resin process, active carbon adsorption, electrolysis method and film point
From method etc., but these methods because it is cumbersome and have secondary pollution, it is all not ideal enough.
On the other hand, the sewage treatment containing rare precious metal is other than it need to consider the factor of Environmental security, resource reclaim
It is that cannot be neglected on the one hand.Traditional rare precious metal secondary resource recycling mainly uses physical-chemical process.Wherein solid waste
The most commonly used is pyrometallurgy technologies for recycling, the basic principle is that heating removing nonmetallic substance using metallurgical furnace high temperature, make dilute
Noble metal is melted in other Metal Melting materials or fused salt, then is separated.Pyrometallurgy has simple process and the rate of recovery
The features such as high, the disadvantage is that energy consumption is high, is easy to produce secondary pollution, and the rare precious metal purity of general pyrogenic process process recycling is not high.
And the method for rare precious metal is recycled from the liquid secondary resource such as waste water, waste liquid then than wide, including chemical precipitation method, electrolysis
Method, ion-exchange, membrane technology partition method and resin adsorption method etc..Although these methods can achieve the effect that it is certain,
Cumbersome when recycling rare precious metal, investment and operating cost are high, and can only generally recycle dilute in high concentration metal waste liquid
Noble metal generates a large amount of secondary waste in removal process, it is therefore desirable to a kind of low cost and the secondary money of rare precious metal of environmental protection
Source circulation utilization method.
Summary of the invention
In order to overcome the above the deficiencies in the prior art, it is improved using HCl treatment Pichia pastoris the present invention provides a kind of
The new method for adsorbing rhodium ion ability in waste water, by adjusting Pichia pastoris in waste liquor PH, adsorption temp, cell concentration, absorption
The adsorption conditions such as time, microbial activity achieve the purpose that adsorb the rhodium ion in waste water.
The first purpose of the invention is to provide a kind of methods of rhodium ion in absorption waste water, and the method is with hydrochloric acid pair
Pichia pastoris is handled, then Pichia pastoris is added with 5~9g dry weight thallus/L waste water final concentration into waste water and carries out rhodium
The absorption of ion.
In one embodiment of the invention, it is described to Pichia pastoris carry out processing be by thallus be added to 0.1~
In the hydrochloric acid of 0.3mol/L, wash 3~4 times.
In one embodiment of the invention, it is 9-11 that the method, which adjusts the pH of waste water,.
In one embodiment of the invention, it is 11 that the method, which adjusts the pH of waste water,.
In one embodiment of the invention, it is 5g/L that the bacterium of the Pichia pastoris is dense.
In one embodiment of the invention, the Pichia pastoris is before being added to waste water by inactivation.
In one embodiment of the invention, the time of the absorption is 120~240min.
In one embodiment of the invention, the temperature of the absorption is 30~40 DEG C.
In one embodiment of the invention, adsorption process is also stirred.
In one embodiment of the invention, the Pichia pastoris is enriched with by thallus, and the enrichment is will to finish red ferment
Mother is seeded in seed culture medium, in 30 DEG C, 16~30h of culture of 200~220rpm, then is forwarded in fermentation medium and trains
It supports.
In one embodiment of the invention, the switching is to be inoculated with seed liquor with 10% volume ratio.
In one embodiment of the invention, the seed culture medium is YEPD fluid nutrient medium；The fermented and cultured
The every L of base contains glycerol 20mL, H3PO420mL, K2SO41g, (NH4)2SO45g, CaSO40.1g, MgSO41g, PTM110mL。
The application that the present invention also provides the methods in sewage treatment.
Beneficial effects of the present invention: the present invention provides a kind of HCl pretreatment Pichia pastoris to be inhaled with improving it to rhodium ion
The method of attached effect, through method of the invention treated Pichia pastoris, rhodium ion adsorption capacity promoted it is obvious, from 20%
It improves to 92.3%；And treated that microbial activity adsorption effect has not significant impact, adsorption rate can reach 90% or more,
And operation of the present invention is easy, at low cost, has important industrial application value.
Detailed description of the invention
Fig. 1 is influence of the pretreatment mode to adsorption rate；
Fig. 2 is the curved line relation for adsorbing pH and adsorption rate；
Fig. 3 is influence of the microbial activity to adsorption rate；
Fig. 4 is influence of the adsorption time to adsorption rate；
Fig. 5 is influence of the cell concentration to adsorption rate and adsorbance；
Fig. 6 is the relation curve of adsorption temp and absorption；
Specific embodiment
Slant medium (g/L): YPD solid medium: peptone 20, yeast extract 10, glucose 20, agar 15.
Seed culture medium: YEPD fluid nutrient medium (g/L): peptone 20, yeast extract 10, glucose 20.
5L fermentation tank culture medium (g/L): glycerol 20mL/L, H3PO420mL/L, K2SO41g, (NH4)2SO45g,
CaSO40.1g, MgSO41g, PTM110mL/L, pH 6.0.
Cell concentration is measured: the absorbance (OD of fermentation liquid is detected at 600nm600), according to curve DCW=0.25
×OD600, obtain dry cell weight.
The measurement of thallus moisture content: the thallus gathered being dried at 60 DEG C, its weight is constantly measured in drying course,
Until its weight no longer changes, this weight is calculated as dry weight；Moisture content is calculated by formula: moisture content=[(one dry weight of weight in wet base)/it is wet
Weight] × 100%.
The measurement of rhodium content: using inductive coupling plasma emission spectrograph (ICP-OES), and measuring method sees reference text
Offer " the different analysis methods of rhodium content in electroplate liquid " (2006 open).
The calculating of adsorbance and adsorption rate: the initial concentration of rhodium ion is initial concentration (mgL in measurement waste water-1), absorption
Rhodium ion concentration is final concentration (mgL in waste water afterwards-1), absorbent concentration be Pichia pastoris dry cell weight (g)/contain rhodium ion
Wastewater volume (L), according to the following formula calculate:
Adsorbance=(initial concentration-final concentration)/absorbent concentration
Adsorption rate=[(initial concentration-final concentration)]/final concentration × 100%
Embodiment 1
From the triangular flask of 500mL that YPD washer chooses that single colonie is inoculated into the seed culture medium containing 50mL, in 30
DEG C, 220rmin-1Culture for 24 hours as the seed liquor of fermentation tank culture.By the seed liquor with the inoculum concentration of 10% (volume ratio)
It is inoculated into 16~36h of culture in the fermentor of the 5L of the fermentation medium containing 1.2L.It is centrifuged 3~10min in 7000rpm, collects bacterium
Body is washed with deionized thallus 2~3 times.
Embodiment 2
The Pichia pastoris of 0.5g (dry weight) is washed 3~4 times with the HCl of 0.1mol/L, 3000~5000rpm centrifugation is received
Collect thallus.It is control with deionized water, using above-mentioned identical method washing, thalline were collected by centrifugation.
The rhodium ion solution of 100mL 50mg/L is prepared in conical flask, the thallus that the above method is collected is added separately to
In conical flask, clog bottleneck with tampon, in 30 DEG C, 200rpm handles 60min, calculate adsorption rate, as a result as shown in Figure 1, without
The Pichia pastoris rhodium ion adsorption rate of HCl processing is 20.0%, and the Pichia pastoris rhodium ion adsorption rate handled through HCl is
66.83%.
Embodiment 3
The Pichia pastoris of 0.5g (dry weight) is washed 3~4 times with the HCl of 0.1mol/L, 3000~5000rpm centrifugation is received
Collect thallus.
The rhodium ion solution of 100mL 50mg/L is prepared in conical flask, adjusts pH=1~11 with NaOH and HCl, it will be upper
State method treated that thallus is added into conical flask, in 30 DEG C, 200rpm handles 120min, calculates adsorption rate.As a result such as Fig. 2
It is shown, pH be 7~11 when, adsorption rate be more than 84.3%, pH be 9~11 when, adsorption rate be 90.2~92.3%.
Embodiment 4
The Pichia pastoris of 0.5g (dry weight) is washed 3~4 times with the HCl of 0.1mol/L, 3000~5000rpm centrifugation is received
Collect thallus, with after washing according to the above method and in the thallus of 121 DEG C of high pressures (0.1MPa) inactivation be control, at identical conditions
Thallus is collected in centrifugation.
The rhodium ion solution of 100mL 50mg/L is prepared in conical flask, treated that thallus is added to cone by the above method
In shape bottle, in 30 DEG C, 200rpm handles 240min, and in 5min, 10min, 30min, 60min, 120min, 180min,
240min measures rhodium ion concentration.As a result as shown in figure 3, the adsorption rate of the thallus absorption rhodium ion after mycelidium and inactivation is equal
Higher than 37.2%, the thallus adsorption rate after handling 60~240min is about 90%, and has no significant difference.
Embodiment 5
The Pichia pastoris of 0.5g (dry weight) is washed 3~4 times with the HCl of 0.1mol/L, 3000~5000rpm centrifugation is received
Collect thallus.
The rhodium ion solution of 100mL 50mg/L is prepared in conical flask, treated that thallus is added to cone by the above method
In shape bottle, in 25~30 DEG C, 200rpm handles 240min, respectively at 5min, 30min, 60min, 90min, 120min,
180min, 240min measurement rhodium ion concentration simultaneously calculate adsorption rate.As a result as shown in figure 4, adsorption time is 120min adsorption rate
Highest, up to 92.3%, the adsorption rate of adsorption time 180min and 240min are respectively 91.2% and 90.6%.
Embodiment 6
The Pichia pastoris of 0.5g (dry weight) is washed 3~4 times with the HCl of 0.1mol/L, 3000~5000rpm centrifugation is received
Collect thallus；According to the above method respectively with the thallus of HCl processing 0.1g (dry weight)~0.9g (dry weight) of 0.1mol/L.
The rhodium ion solution of several bottles of 100mL 50mg/L is prepared in conical flask, by the above method treated thallus point
Jia Ru not be into conical flask, in 25~30 DEG C, 200rpm handles 120min, measures rhodium ion concentration and calculates adsorption rate.As a result
As shown in figure 5, adsorption rate is up to 92.1% or more when cell concentration is 5g/L~9g/L.
Embodiment 7
The Pichia pastoris of 0.5g (dry weight) is washed 3~4 times with the HCl of 0.1mol/L, 3000~5000rpm centrifugation is received
Collect thallus.
The rhodium ion solution of 100mL 50mg/L is prepared in conical flask, treated that thallus is added to cone by the above method
In shape bottle, respectively at 15~45 DEG C (one gradient of every 5 DEG C of settings), 200rpm handles 120min, measures rhodium ion concentration and counts
Calculate adsorption rate.As a result as shown in fig. 6, adsorption effect is ideal when temperature is 30~40 DEG C, about 90% or more；Adsorption temp is
Adsorption rate highest at 30 DEG C, up to 91.9%.
Embodiment 8
The Pichia pastoris of 0.5~0.9g (dry weight) is washed 3~4 times with the HCl of 0.1~0.3mol/L, 3000~
5000rpm centrifugation, collects thallus.
The rhodium ion solution of 100mL 50mg/L is prepared in conical flask, treated that thallus is added to cone by the above method
In shape bottle, pH9~11 are adjusted, in 30~40 DEG C, 200~220rpm handles 120~240min, measures rhodium ion concentration and calculates
Adsorption rate.The results show that treated that thallus reaches as high as the adsorption rate of rhodium ion under above-mentioned adsorption conditions by HCl
92.3%.
Although the present invention has been described by way of example and in terms of the preferred embodiments, it is not intended to limit the invention, any to be familiar with this skill
The people of art can do various change and modification, therefore protection model of the invention without departing from the spirit and scope of the present invention
Enclosing subject to the definition of the claims.
Claims (10)
1. it is a kind of absorption waste water in rhodium ion method, which is characterized in that the method be with hydrochloric acid to Pichia pastoris at
Reason, then Pichia pastoris is added with 5~9g thallus/L waste water final concentration into waste water to the absorption for carrying out rhodium ion.
2. the method according to claim 1, wherein it is described to Pichia pastoris carry out processing be by Pichia pastoris
Thallus washs 3~4 times in the hydrochloric acid of 0.1~0.3mol/L.
3. method according to claim 1 or 2, which is characterized in that the pH that the method adjusts waste water is 9-11.
4. according to the method described in claim 3, it is characterized in that, the bacterium of Pichia pastoris is dense for 5g/L.
5. according to claim 1,2,4 any method, which is characterized in that the time of absorption is 120~240min.
6. according to the method described in claim 5, it is characterized in that, the temperature of absorption is 30~40 DEG C.
7. according to claim 1,2,4,6 any method, which is characterized in that adsorption process is also stirred.
8. the method according to the description of claim 7 is characterized in that Pichia pastoris is before being added to waste water by inactivation.
9. according to the method described in claim 8, the enrichment is will to finish it is characterized in that, Pichia pastoris is enriched with by thallus
Red yeast-inoculated in 30 DEG C, 16~30h of culture of 200~220rpm, then is forwarded in fermentation medium into seed culture medium
Enrichment culture.
10. claim 1-4, application of 6, the 8 any the methods in sewage treatment.
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| CN107381830A (en) * | 2017-07-19 | 2017-11-24 | 宜兴市科兴合金材料有限公司 | The collection method of rhodium ion in a kind of waste water |
| CN107879485A (en) * | 2017-12-13 | 2018-04-06 | 合肥郑国生物科技有限公司 | A kind of biological adsorption recovery method of heavy metals in industrial wastewater |
| CN108031445A (en) * | 2017-12-13 | 2018-05-15 | 合肥郑国生物科技有限公司 | A kind of preparation method and application method of the Pichia kudriavezii adsorbent of quick adsorption heavy metal |
| CN108244460A (en) * | 2018-01-31 | 2018-07-06 | 淄博职业学院 | It is a kind of to improve method of the saccharomyces cerevisiae to Metal Ions in Foodstuffs adsorption capacity |
| CN114669281B (en) * | 2022-04-02 | 2023-04-18 | 中南大学 | PEI modified yeast residue-based platinum-palladium-rhodium adsorption material, preparation method and industrial wastewater treatment method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1113890A (en) * | 1992-12-09 | 1995-12-27 | 泰克尼翁研究发展基金有限公司 | Process for the removal of species containing metallicions from effluents |
| CN102557271A (en) * | 2010-12-17 | 2012-07-11 | 上海市长宁区少年科技指导站 | Method for treating heavy-metal-polluted water through waste yeast |
| WO2015121798A1 (en) * | 2014-02-11 | 2015-08-20 | Bortoli Elio Fabio | Biocatalytic composition for treatment of substrates |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1113890A (en) * | 1992-12-09 | 1995-12-27 | 泰克尼翁研究发展基金有限公司 | Process for the removal of species containing metallicions from effluents |
| CN102557271A (en) * | 2010-12-17 | 2012-07-11 | 上海市长宁区少年科技指导站 | Method for treating heavy-metal-polluted water through waste yeast |
| WO2015121798A1 (en) * | 2014-02-11 | 2015-08-20 | Bortoli Elio Fabio | Biocatalytic composition for treatment of substrates |
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