CN106365332B - A method of utilizing rhodium ion in chlorination Calcium treatment Pichia pastoris absorption waste water - Google Patents
A method of utilizing rhodium ion in chlorination Calcium treatment Pichia pastoris absorption waste water Download PDFInfo
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- CN106365332B CN106365332B CN201611025072.7A CN201611025072A CN106365332B CN 106365332 B CN106365332 B CN 106365332B CN 201611025072 A CN201611025072 A CN 201611025072A CN 106365332 B CN106365332 B CN 106365332B
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- pichia pastoris
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/20—Heavy metals or heavy metal compounds
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Abstract
The invention discloses a kind of methods using rhodium ion in chlorination Calcium treatment Pichia pastoris absorption waste water, belong to sewage treatment field.Method of the invention is by carrying out CaCl to Pichia pastoris thallus2Processing improves the adsorption capacity to rhodium ion in waste water.The CaCl of 0.1~0,3mol/L of the present invention2The thallus of processing, and thallus is added into the sewage containing rhodium ion, adjust 6~16g/L of cell concentration, adjust pH 9~11, in 30~45 DEG C, 200~220rpm handles 120~240min, can adsorb in sewage 97.9% rhodium ion, this method is easy to operate, at low cost, has important industrial application value.
Description
Technical field
The present invention relates to a kind of methods using rhodium ion in chlorination Calcium treatment Pichia pastoris absorption waste water, belong at sewage
Reason field.
Background technique
To prevent the pollution of the environment, industrial wastewater is needed by processing, and can just be discharged after reaching standard.Processing is containing a huge sum of money
The sewage water treatment method of category has very much, such as chemical precipitation method, ion-exchange-resin process, active carbon adsorption, electrolysis method and film point
From method etc., but these methods because it is cumbersome and have secondary pollution, it is all not ideal enough.
On the other hand, the sewage treatment containing rare precious metal is other than it need to consider the factor of Environmental security, resource reclaim
It is that cannot be neglected on the one hand.Traditional rare precious metal secondary resource recycling mainly uses physical-chemical process.Wherein solid waste
The most commonly used is pyrometallurgy technologies for recycling, the basic principle is that heating removing nonmetallic substance using metallurgical furnace high temperature, make dilute
Noble metal is melted in other Metal Melting materials or fused salt, then is separated.Pyrometallurgy has simple process and the rate of recovery
The features such as high, the disadvantage is that energy consumption is high, is easy to produce secondary pollution, and the rare precious metal purity of general pyrogenic process process recycling is not high.
And other methods such as chemical precipitation method, electrolysis method, the ion of rare precious metal are recycled from the liquid secondary resource such as waste water, waste liquid
Exchange process, membrane technology partition method and resin adsorption method etc., although can achieve the effect that it is certain, when recycling rare precious metal,
Cumbersome, investment and operating cost are high, and can only generally recycle the rare precious metal in high concentration metal waste liquid, removal process
It is middle to generate a large amount of secondary waste, it is therefore desirable to a kind of low cost and environmentally friendly rare precious metal secondary resource circulation utilization method.
Summary of the invention
In order to overcome the above the deficiencies in the prior art, the present invention provides a kind of using chlorination Calcium treatment Pichia pastoris to increase
Its strong new method for adsorbing rhodium ion in waste water, by control Pichia pastoris in waste liquor PH, adsorption temp, cell concentration, absorption
The adsorption conditions of time, microbial activity etc. achieve the purpose that adsorb rhodium ion in waste water.
The first purpose of the invention is to provide a kind of methods of rhodium ion in absorption waste water, and the method is to use CaCl2
Pichia pastoris is handled, then Pichia pastoris is added with 6~16g dry weight thallus/L waste water final concentration into waste water and is carried out
The absorption of rhodium ion.
In one embodiment of the invention, it is described to Pichia pastoris carry out processing be thallus is added to 0.1~
The CaCl of 0.3mol/L2In, stir 6-24h.
In one embodiment of the invention, it is 9-11 that the method, which adjusts the pH of sewage,.
In one embodiment of the invention, it is 11 that the method, which adjusts the pH of sewage,.
In one embodiment of the invention, the Pichia pastoris is before being added to waste water by inactivation.
In one embodiment of the invention, the time of the absorption is 120~240min.
In one embodiment of the invention, the cell concentration of the absorption is 10g/L.
In one embodiment of the invention, the temperature of the absorption is 30~45 DEG C.
In one embodiment of the invention, adsorption process is also stirred.
In one embodiment of the invention, the Pichia pastoris is enriched with by thallus, and the enrichment is will to finish red ferment
Mother is seeded in seed culture medium, in 30 DEG C, 16~30h of culture of 200~220rpm, then is forwarded in fermentation medium and trains
It supports.
In one embodiment of the invention, the switching is to be inoculated with seed liquor with 10% volume ratio.
In one embodiment of the invention, the seed culture medium is YEPD fluid nutrient medium；The fermented and cultured
The every L of base contains glycerol 20mL, H3PO420mL, K2SO41g, (NH4)2SO45g, CaSO40.1g, MgSO41g, PTM1
10mL。
Beneficial effects of the present invention: the present invention provides a kind of CaCl2Pichia pastoris is pre-processed to improve it to rhodium ion
The method of adsorption effect, through method of the invention treated Pichia pastoris, rhodium ion adsorption capacity promoted it is obvious, from
20.3% improves to 97.9%；And treated that microbial activity has not significant impact adsorption effect, adsorption rate can reach
90% or more.Operation of the present invention is easy, at low cost, has important industrial application value.
Detailed description of the invention
Fig. 1 is influence of the pretreatment mode to adsorption rate；
Fig. 2 is the curved line relation for adsorbing pH and adsorption rate；
Fig. 3 is influence of the microbial activity to adsorption rate；
Fig. 4 is influence of the adsorption time to adsorption rate；
Fig. 5 is influence of the cell concentration to adsorption rate and adsorbance；
Fig. 6 is the relation curve of adsorption temp and absorption；
Specific embodiment
Slant medium (g/L): YPD solid medium: peptone 20, yeast extract 10, glucose 20, agar 15.
Seed culture medium: YEPD fluid nutrient medium (g/L): peptone 20, yeast extract 10, glucose 20.
5L fermentor initial medium (g/L): glycerol 20 (mL/L), H3PO420 (mL/L), K2SO41, (NH4)2SO4
5, CaSO40.1, MgSO41, PTM110 (mL/L), pH 6.0.
Cell concentration is measured: the absorbance (OD of fermentation liquid is detected at 600nm600), according to curve DCW=0.25
×OD600, obtain dry cell weight.
The measurement of thallus moisture content: the thallus gathered being dried at 60 DEG C, its weight is constantly measured in drying course,
Until its weight no longer changes, this weight is calculated as dry weight；Moisture content is calculated by formula: moisture content=[(one dry weight of weight in wet base)/it is wet
Weight] × 100%.
The measurement of rhodium content: using inductive coupling plasma emission spectrograph (ICP-OES), and measuring method sees reference text
Offer " the different analysis methods of rhodium content in electroplate liquid " (2006 open).
The calculating of adsorbance and adsorption rate: the initial concentration of rhodium ion is initial concentration (mgL in measurement waste water-1), absorption
Rhodium ion concentration is final concentration (mgL in waste water afterwards-1), absorbent concentration is that (g rolls over Pichia pastoris thallus according to moisture content
Dry weight is synthesized to calculate)/the wastewater volume (L) containing rhodium ion, it calculates according to the following formula:
Adsorbance=concentration of (initial concentration-the final concentration)/Pichia pastoris in waste water
Adsorption rate=[(initial concentration-final concentration)]/final concentration × 100%
Embodiment 1
From the triangular flask of 500mL that YPD washer chooses that single colonie is inoculated into the seed culture medium containing 50mL, in 30
DEG C, 220rmin-1Culture for 24 hours as the seed liquor of fermentation tank culture.By the seed liquor with the inoculum concentration of 10% (volume ratio)
It is inoculated into 16~36h of culture in the fermentor of the 5L of the fermentation medium containing 1.2L.It is centrifuged 3~10min in 7000rpm, collects bacterium
Body is washed with deionized thallus 2~3 times.
Embodiment 2
With the CaCl of 0.2mol/L2The Pichia pastoris of 1g (dry weight) is suspended and is mixed, is slowly stirred 6- in magnetic stirring apparatus
For 24 hours, 3000~5000rpm is centrifuged, and is washed with deionized 3~4 times, thalline were collected by centrifugation.With the deionized water of same volume
For control, using above-mentioned identical method stirring, washing, thalline were collected by centrifugation.
The rhodium ion solution of 100mL 50mg/L is prepared in conical flask, the thallus that the above method is collected is added separately to
In conical flask, clog bottleneck with tampon, in 30 DEG C, 200rpm handles 60min, calculate adsorption rate, as a result as shown in Figure 1, without
CaCl2The Pichia pastoris rhodium ion adsorption rate of processing is 20.3%, through CaCl2The Pichia pastoris rhodium ion adsorption rate of processing is
78.2%.
Embodiment 3
With the CaCl of 0.2mol/L2The Pichia pastoris of 1g (dry weight) is suspended and is mixed, is slowly stirred 6- in magnetic stirring apparatus
For 24 hours, 3000~5000rpm is centrifuged, and is washed with deionized 3~4 times, thalline were collected by centrifugation.
The rhodium ion solution of 100mL 50mg/L is prepared in conical flask, adjusts pH=1~11 with NaOH and HCl, it will be upper
State method treated that thallus is added into conical flask, in 30 DEG C, 200rpm handles 120min, calculates adsorption rate.As a result such as Fig. 2
It is shown, pH be 7~11 when, adsorption rate be more than 84.9%, pH be 9~11 when, adsorption rate be 92.5~97.1%.
Embodiment 4
With the CaCl of 0.2mol/L2The Pichia pastoris of 1g (dry weight) is suspended and is mixed, is slowly stirred 6- in magnetic stirring apparatus
For 24 hours, 3000~5000rpm is centrifuged, and is washed with deionized 3~4 times, thalline were collected by centrifugation.To stir, wash according to the above method
It is afterwards and in the thallus of 121 DEG C of high pressures (0.1MPa) inactivation control, is centrifuged at identical conditions, collects thallus.
The rhodium ion solution of 100mL 50mg/L is prepared in conical flask, treated that thallus is added to cone by the above method
In shape bottle, in 30 DEG C, 200rpm handles 240min, and in 5min, 10min, 30min, 60min, 120min, 180min,
240min measures rhodium ion concentration, calculates adsorption rate.As a result as shown in figure 3, after mycelidium and inactivation thallus absorption rhodium from
The adsorption rate of son is above 37.2%, and the thallus adsorption rate after handling 60~240min is about 90%, and has no significant difference.
Embodiment 5
With the CaCl of 0.2mol/L2The Pichia pastoris of 1g (dry weight) is suspended and is mixed, is slowly stirred 6- in magnetic stirring apparatus
For 24 hours, 3000~5000rpm is centrifuged, and is washed with deionized 3~4 times, thalline were collected by centrifugation.
The rhodium ion solution of 100mL 50mg/L is prepared in conical flask, treated that thallus is added to cone by the above method
In shape bottle, in 25~30 DEG C, 200rpm handles 240min, respectively at 5min, 30min, 60min, 90min, 120min,
180min, 240min measurement rhodium ion concentration simultaneously calculate adsorption rate.As a result as shown in figure 4, adsorption time is 120min adsorption rate
Highest, up to 97.3%, the adsorption rate of adsorption time 180min and 240min are respectively 95.2% and 94.6%, adsorption time
The adsorption rate of 90min is 86.3%.
Embodiment 6
With the CaCl of 0.2mol/L2The Pichia pastoris of 1g (dry weight) is suspended and is mixed, is slowly stirred 6- in magnetic stirring apparatus
For 24 hours, 3000~5000rpm is centrifuged, and is washed with deionized 3~4 times, thalline were collected by centrifugation.It uses respectively according to the above method
The CaCl of 0.2mol/L2Handle the thallus of 0.2g (dry weight)~1.6g (dry weight).
The rhodium ion solution of several bottles of 100mL 50mg/L is prepared in conical flask, by the above method treated thallus point
Jia Ru not be into conical flask, in 25~30 DEG C, 200rpm handles 120min, measures rhodium ion concentration and calculates adsorption rate.As a result
As shown in figure 5, adsorption rate is up to 92.1% or more when cell concentration is 6g/L~16g/L, wherein when concentration is 10g/L, absorption
Rate highest is 97.2%.
Embodiment 7
With the CaCl of 0.2mol/L2The Pichia pastoris of 1g (dry weight) is suspended and is mixed, is slowly stirred 6- in magnetic stirring apparatus
For 24 hours, 3000~5000rpm is centrifuged, and is washed with deionized 3~4 times, thalline were collected by centrifugation.
The rhodium ion solution of 100mL 50mg/L is prepared in conical flask, treated that thallus is added to cone by the above method
In shape bottle, respectively at 15~45 DEG C (one gradient of every 5 DEG C of settings), 200rpm handles 120min, measures rhodium ion concentration and counts
Calculate adsorption rate.As a result as shown in fig. 6, adsorption effect is ideal when temperature is 30~45 DEG C, about 91.9% or more；Adsorption temp
Adsorption rate highest when being 30 DEG C, up to 97.9%.
Embodiment 8
With the CaCl of 0.1~0.3mol/L2The Pichia pastoris of 0.6~1.6g (dry weight) is suspended and is mixed, in magnetic agitation
Device is slowly stirred 6-24h, and 3000~5000rpm centrifugation is washed with deionized 3~4 times, thalline were collected by centrifugation.
The rhodium ion solution of 100mL 50mg/L is prepared in conical flask, treated that thallus is added to cone by the above method
In shape bottle, pH 9~11 is adjusted, in 30~45 DEG C, 200~220rpm handles 120~240min, measures rhodium ion concentration and counts
Calculate adsorption rate.The results show that CaCl2Treated, and thallus reaches as high as the adsorption rate of rhodium ion under above-mentioned adsorption conditions
97.9%.
Although the present invention has been described by way of example and in terms of the preferred embodiments, it is not intended to limit the invention, any to be familiar with this skill
The people of art can do various change and modification, therefore protection model of the invention without departing from the spirit and scope of the present invention
Enclosing subject to the definition of the claims.
Claims (9)
1. a kind of method of rhodium ion in absorption waste water, which is characterized in that the method is to use CaCl2At Pichia pastoris
Reason, then Pichia pastoris is added with 6~16g dry weight thallus/L waste water final concentration into waste water to the absorption for carrying out rhodium ion,
In, the pH that the method adjusts waste water is 9-11；It is described to Pichia pastoris carry out processing be thallus is added to 0.1~
The CaCl of 0.3mol/L2In, stir 6-24h.
2. the method according to claim 1, wherein being to be added to finish with 10g dry weight thallus/L waste water final concentration
Red yeast carries out rhodium ion absorption.
3. the method according to claim 1, wherein the time of absorption is 120~240min.
4. method according to claim 1 or 3, which is characterized in that the temperature of absorption is 30~45 DEG C.
5. according to the method described in claim 4, it is characterized in that, adsorption process is also stirred.
6. the method according to claim 1, wherein Pichia pastoris is before being added to waste water by inactivation.
7. according to the method described in claim 4, it is characterized in that, Pichia pastoris is before being added to waste water by inactivation.
8. according to the method described in claim 6, the enrichment is it is characterized in that, the Pichia pastoris is enriched with by thallus
Pichia pastoris is seeded in seed culture medium, in 30 DEG C, 200~220rpm cultivates 16~30h, then is forwarded to fermentation medium
Middle enrichment culture.
9. application of any method of claim 1,3,6,8 in sewage treatment.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1113890A (en) * | 1992-12-09 | 1995-12-27 | 泰克尼翁研究发展基金有限公司 | Process for the removal of species containing metallicions from effluents |
| CN102557271A (en) * | 2010-12-17 | 2012-07-11 | 上海市长宁区少年科技指导站 | Method for treating heavy-metal-polluted water through waste yeast |
| WO2015121798A1 (en) * | 2014-02-11 | 2015-08-20 | Bortoli Elio Fabio | Biocatalytic composition for treatment of substrates |
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- 2016-11-22 CN CN201611025072.7A patent/CN106365332B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1113890A (en) * | 1992-12-09 | 1995-12-27 | 泰克尼翁研究发展基金有限公司 | Process for the removal of species containing metallicions from effluents |
| CN102557271A (en) * | 2010-12-17 | 2012-07-11 | 上海市长宁区少年科技指导站 | Method for treating heavy-metal-polluted water through waste yeast |
| WO2015121798A1 (en) * | 2014-02-11 | 2015-08-20 | Bortoli Elio Fabio | Biocatalytic composition for treatment of substrates |
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