CN105734037A - Waste water treatment agent and application thereof - Google Patents
Waste water treatment agent and application thereof Download PDFInfo
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- CN105734037A CN105734037A CN201610112186.9A CN201610112186A CN105734037A CN 105734037 A CN105734037 A CN 105734037A CN 201610112186 A CN201610112186 A CN 201610112186A CN 105734037 A CN105734037 A CN 105734037A
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- saccharomyces cerevisiae
- polyvinyl alcohol
- screen cloth
- adsorption
- waste water
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- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 14
- 238000004065 wastewater treatment Methods 0.000 title abstract 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 40
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 40
- 238000000034 method Methods 0.000 claims abstract description 30
- 239000004372 Polyvinyl alcohol Substances 0.000 claims abstract description 28
- 229920002451 polyvinyl alcohol Polymers 0.000 claims abstract description 28
- 210000001822 immobilized cell Anatomy 0.000 claims abstract description 25
- 229910001385 heavy metal Inorganic materials 0.000 claims abstract description 21
- 239000002351 wastewater Substances 0.000 claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 241000589630 Pseudomonas pseudoalcaligenes Species 0.000 claims abstract description 13
- 239000011259 mixed solution Substances 0.000 claims abstract description 13
- 235000010410 calcium alginate Nutrition 0.000 claims abstract description 8
- 239000000648 calcium alginate Substances 0.000 claims abstract description 8
- 229960002681 calcium alginate Drugs 0.000 claims abstract description 8
- OKHHGHGGPDJQHR-YMOPUZKJSA-L calcium;(2s,3s,4s,5s,6r)-6-[(2r,3s,4r,5s,6r)-2-carboxy-6-[(2r,3s,4r,5s,6r)-2-carboxylato-4,5,6-trihydroxyoxan-3-yl]oxy-4,5-dihydroxyoxan-3-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylate Chemical compound [Ca+2].O[C@@H]1[C@H](O)[C@H](O)O[C@@H](C([O-])=O)[C@H]1O[C@H]1[C@@H](O)[C@@H](O)[C@H](O[C@H]2[C@H]([C@@H](O)[C@H](O)[C@H](O2)C([O-])=O)O)[C@H](C(O)=O)O1 OKHHGHGGPDJQHR-YMOPUZKJSA-L 0.000 claims abstract description 8
- 238000001816 cooling Methods 0.000 claims abstract description 7
- 239000000243 solution Substances 0.000 claims abstract description 7
- 229910019142 PO4 Inorganic materials 0.000 claims abstract description 6
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical class OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims abstract description 6
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims abstract description 6
- 239000010452 phosphate Substances 0.000 claims abstract description 6
- 238000002156 mixing Methods 0.000 claims abstract description 4
- 238000001179 sorption measurement Methods 0.000 claims description 58
- 241000168225 Pseudomonas alcaligenes Species 0.000 claims description 24
- 239000004744 fabric Substances 0.000 claims description 23
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 19
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 15
- 239000008103 glucose Substances 0.000 claims description 15
- 230000008569 process Effects 0.000 claims description 15
- 239000001888 Peptone Substances 0.000 claims description 12
- 108010080698 Peptones Proteins 0.000 claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 229940041514 candida albicans extract Drugs 0.000 claims description 12
- 238000000855 fermentation Methods 0.000 claims description 12
- 230000004151 fermentation Effects 0.000 claims description 12
- 235000019319 peptone Nutrition 0.000 claims description 12
- 239000012138 yeast extract Substances 0.000 claims description 12
- 239000007836 KH2PO4 Substances 0.000 claims description 9
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 9
- 238000009423 ventilation Methods 0.000 claims description 9
- 238000005276 aerator Methods 0.000 claims description 8
- 229910052564 epsomite Inorganic materials 0.000 claims description 8
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 8
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 6
- 244000068988 Glycine max Species 0.000 claims description 6
- 235000010469 Glycine max Nutrition 0.000 claims description 6
- 229910021577 Iron(II) chloride Inorganic materials 0.000 claims description 6
- 239000001110 calcium chloride Substances 0.000 claims description 6
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 6
- NMCUIPGRVMDVDB-UHFFFAOYSA-L iron dichloride Chemical compound Cl[Fe]Cl NMCUIPGRVMDVDB-UHFFFAOYSA-L 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 238000012807 shake-flask culturing Methods 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 125000003647 acryloyl group Chemical group O=C([*])C([H])=C([H])[H] 0.000 claims description 5
- 150000002500 ions Chemical class 0.000 claims description 5
- 229960005486 vaccine Drugs 0.000 claims description 5
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 4
- 239000012531 culture fluid Substances 0.000 claims description 3
- 238000011049 filling Methods 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims 1
- 235000019341 magnesium sulphate Nutrition 0.000 claims 1
- 235000019796 monopotassium phosphate Nutrition 0.000 claims 1
- 229910052925 anhydrite Inorganic materials 0.000 abstract description 2
- 239000011324 bead Substances 0.000 abstract 4
- 241001052560 Thallis Species 0.000 abstract 2
- -1 acrylyl Chemical group 0.000 abstract 1
- OSGAYBCDTDRGGQ-UHFFFAOYSA-L calcium sulfate Chemical compound [Ca+2].[O-]S([O-])(=O)=O OSGAYBCDTDRGGQ-UHFFFAOYSA-L 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- ZIUHHBKFKCYYJD-UHFFFAOYSA-N n,n'-methylenebisacrylamide Chemical compound C=CC(=O)NCNC(=O)C=C ZIUHHBKFKCYYJD-UHFFFAOYSA-N 0.000 abstract 1
- 244000005700 microbiome Species 0.000 description 6
- 239000003463 adsorbent Substances 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 230000007246 mechanism Effects 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 229910021645 metal ion Inorganic materials 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000008187 granular material Substances 0.000 description 2
- 230000002779 inactivation Effects 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241001597008 Nomeidae Species 0.000 description 1
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 1
- 241000588902 Zymomonas mobilis Species 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 238000000975 co-precipitation Methods 0.000 description 1
- 238000010668 complexation reaction Methods 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000004134 energy conservation Methods 0.000 description 1
- 239000007863 gel particle Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 239000004021 humic acid Substances 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000010814 metallic waste Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000006722 reduction reaction Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 235000019355 sepiolite Nutrition 0.000 description 1
- 229910052624 sepiolite Inorganic materials 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 229910000859 α-Fe Inorganic materials 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/04—Enzymes or microbial cells immobilised on or in an organic carrier entrapped within the carrier, e.g. gel or hollow fibres
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- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/08—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a synthetic polymer
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Abstract
The invention discloses a waste water treatment agent. The waste water treatment agent is prepared by using the following steps: 1, mixed fermenting, culturing and collecting pseudomonas pseudoalcaligenes and saccharomyces cerevisiae thalli; 2, mixing polyvinyl alcohol, calcium alginate, acrylyl and water according to a mass ratio of (6-15):(0.5-1.5):(3-5):100 to obtain a polyvinyl alcohol hydrosol, and adding the collected pseudomonas pseudoalcaligenes and saccharomyces cerevisiae thalli into the polyvinyl alcohol hydrosol to form a mixed solution; 3, adding the mixed solution into a saturated boric acid solution containing N'-methylene bisacrylamide CaSO4 by using a peristaltic pump to form a gel bead, cooling and unfreezing the gel bead, then cooling and unfreezing the gel bead again, placing in phosphate with the mass concentration of 0.2-0.5 g/L to obtain an immobilized cell bead with a spherical appearance and an internal porous mesh structure. The invention also discloses a method for treating waste water by using the treatment agent. Different heavy metals are well adsorbed.
Description
Technical field
The present invention relates to sewage treatment area, particularly to a kind of waste water treating agent and application thereof.
Background technology
At present, process for treating heavy-metal waste water mainly has three classes: chemical reaction method, concentrating and separating method and biosorption process.The first kind is that heavy metal ions in wastewater includes neutralization precipitation method, sulphide precipitation, ferrite coprecipitation, chemical reduction method, electrochemical reducing and high molecular heavy metals trapping agent method etc. by the method that chemical reaction removes；Equations of The Second Kind is to make the heavy metal in waste water carry out the method concentrating, separating under conditions of not changing its chemical form, including adsorbent, ion exchange and membrance separation etc.；3rd class biosorption process is a heavy metal-polluted method for treating water of new development in this year, by be microorganism and derivant thereof the process of carrying out adsorbing heavy metal in water.
Compared with tradition adsorption method, biosorption process has the advantage that 1) at low concentrations, metal can be by the removal of selectivity；2) energy-conservation, treatment effeciency is high；3) recovery heavy metal 5 it is easily isolated) adsorbent is prone to regeneration.
It is critical only that of biosorption technology selects suitable biological adsorption agent.Biological adsorption agent many employings live body and inactivation biological cell two kinds.Compared with inactivation biological cell, the adsorbance of living body biological cell absorption is relatively big, and its adsorption process divides 2 stages.1st stage is unrelated with metabolism, for biological adsorption process, carries out very fast, in the process, metal ion can be exchanged with ion by coordination, chelating, physical absorption and microdeposit etc. active one or more be combined to cell surface；2nd stage was biological cumulative process, carried out relatively slow, and in the process, metal is transported to intracellular.
But, in current document, many utilize single kind microorganism as biological adsorption agent, or how by deactivated microorganism and conventional adsorbent, form as activated carbon, humic acids, meerschaum, polysaccharide resins etc. are mixed with.The mechanism of biological adsorption is often different because of the difference of strain, and the source of biomaterial is quite varied, thus selects suitable strain to be particularly important as adsorbent.
Summary of the invention
The present invention provides a kind of method processing waste water, utilizes inhomogeneous microorganism live bacteria, under the effect of different adsorption mechanisms, to different heavy metal (Cd2+,Hg2+,Cu2+,Zn2+,Ni2+,Ag2+,Pb2+) adsorb.
In order to solve the problems referred to above, the technical solution used in the present invention is such that a kind of adsorbent for processing heavy metal wastewater thereby, it is characterised in that adopts and is prepared from the following method,
1) mixing fermentation culture and collection pseudomonas pseudoalcaligenes and saccharomyces cerevisiae thalline:
A) cultivation of saccharomyces cerevisiae seed liquor
Saccharomyces cerevisiae strain on inclined-plane is inoculated in primary-seed medium and carries out shake-flask culture, at 27-30 DEG C, after rotating speed 260rmp cultivates 24h, be inoculated in secondary seed medium and carry out fermentor cultivation, at 30-32 DEG C, rotating speed 260-280rmp, ventilation 2.5～3m3/ h obtains the seed liquor of saccharomyces cerevisiae after cultivating 24-36h, wherein said primary-seed medium is: yeast extract 5g/L, peptone 6g/L, glucose 20g/L；Secondary seed medium is: glucose 40-50g/L, yeast extract 5-8g/L, peptone 5-8g/L, (NH4)2SO42-3g/L, MgSO4·7H2O 0.2 5-0.3g/L, NaCl 0.15-0.2g/L, KH2PO42.8-3g/L；
B) cultivation of Pseudomonas alcaligenes seed liquor
Pseudomonas alcaligenes strain on inclined-plane is inoculated in primary-seed medium and carries out shake-flask culture, at 27-30 DEG C, after rotating speed 160-180rmp cultivates 24h, it is inoculated in secondary seed medium and carries out fermentor cultivation, at 27-30 DEG C, rotating speed 160-180rmp, ventilation 2.5～3m3/h is cultivated 24-36h, is obtained the seed liquor of Pseudomonas alcaligenes, wherein, described primary-seed medium is: glucose 20-30g/L, yeast extract 5-8g/L, peptone 6-8g/L；Described secondary seed medium is: glucose 20-30g/L, soybean cake powder 10-12g/L, KNO31-1.2g/L, KH2PO41-1.2g/L, FeCl2·6H2O 0.05-0.08g/L,CaCl2·7H2O0.02-0.04g/L, MgSO4·7H2O 1-1.2g/L；
C) mixed fermentation
By step 1) the saccharomyces cerevisiae seed liquor of gained and step 2) the Pseudomonas alcaligenes seed liquor 1-3:1-3 by volume of gained mixes, proceed to fermentation medium is carried out High Density Cultivation with the inoculum concentration of 10-20%, at 30 DEG C, rotating speed 220-260rmp, ventilation 5～10m3/ h cultivates 36-48h, and the culture fluid after fermenting stands, and centrifugal enrichment thalline is pseudomonas pseudoalcaligenes and saccharomyces cerevisiae thalline, wherein, described fermentation medium is: glucose sugar 80-100g/L, yeast extract 5-10g/L, peptone 5-10g/L, soybean cake powder 10-12g/L, KNO31-1.2g/L, KH2PO42-2.5g/L, FeCl2·6H2O 0.05-0.08g/L, CaCl2·7H2O 0.02-0.04g/L, MgSO4·7H2O 1.5-1.8g/L, NaCl 0.15-0.18g/L；
2) preparation of immobilized cell ball: A) polyvinyl alcohol, calcium alginate, acryloyl are mixed with water in mass ratio 6～15:0.5～1.5:3～5:100 ratio after, obtain the polyvinyl alcohol hydrosol, the pseudomonas pseudoalcaligenes collected and saccharomyces cerevisiae thalline are added to the polyvinyl alcohol hydrosol, forms mixed solution, wherein, Pseudomonas alcaligenes and the saccharomyces cerevisiae mixed vaccine of 4-6g weight in wet base are added during the addition of Pseudomonas alcaligenes and saccharomyces cerevisiae thalline meets the polyvinyl alcohol hydrosol of every 100mL；B) mixed solution peristaltic pump is joined containing 3～5w/v%N '-methylene-bisacrylamide and 1-2w/v%CaSO4Saturated boric acid solution in, form gel ball, 5-10h is cooled down under the conditions of placing-20 DEG C, thawed at room temperature, cooling down 1-2h under the conditions of being placed in-10 DEG C the most again, after thawed at room temperature, it is in 0.2～0.5g/L phosphate that gel ball puts into mass concentration, it is spherical for obtaining profile, the internal immobilized cell ball in porous network structure.
Present invention also offers a kind of method applying above-mentioned biological adsorption agent to process heavy metal wastewater thereby, comprise the steps:
1) preparation of biological adsorption device: described biological adsorption device is formed by many adsorption column concatenations, it is provided with water inlet on described adsorption column lower sides, it is provided with overfall in adsorption column upper portion side wall, upper screen cloth and lower screen cloth it is provided with in adsorption column, upper screen cloth position is higher than the position at water inlet place, lower screen cloth position is less than the position at overfall place, described adsorption column sidewall is with upper, immobilized cell ball it is filled with in the adsorption space that lower screen cloth is surrounded, immobilized cell ball is 50-80% at the total filling rate of adsorption space, also set up in this adsorption space and be provided with annular guide shell, the top and bottom of described annular guide shell do not contact with upper screen cloth and lower screen cloth respectively, aerator it is provided with bottom adsorption column, this aerator is positioned at below annular guide shell.
2) process of waste water: by concentration of heavy metal ion adjustment≤800mg/L initial in heavy metal wastewater thereby, after PH is adjusted to 5-7, flow through biological adsorption device successively, the hydraulic detention time 0.5-1h in every adsorption column.
Present invention employs conventional fixation support-polyvinyl alcohol, Pseudomonas alcaligenes is fixed with saccharomyces cerevisiae, it has the series of advantages such as intensity is high, chemical stability is good, Resistance to microbes performance strong, nontoxic to microorganism, cheap, introduce calcium alginate on this basis and solve the agglomeration problem of granule, enhance the balling-up ability of polyvinyl alcohol gel, it addition, calcium alginate and CaSO4Addition strengthen gel particle intensity, the most also introduce acrylamide polymerization reaction, it is allowed to and surface formation polyacrylamide network structure internal at polyvinyl alcohol gel granule, effectively improve the water-soluble dilatancy of polyvinyl alcohol gel, polyvinyl alcohol immobilized microorganism is made to have comparatively ideal physics (size) and mechanical performance, considerably increase Pseudomonas alcaligenes and the saccharomyces cerevisiae toleration to toxic metal ions, reach the effect recycled.
With prior art ratio, the present invention have chosen Pseudomonas alcaligenes and saccharomyces cerevisiae viable bacteria as biological adsorption agent, wherein saccharomyces cerevisiae is the fungus that most metal ions is respectively provided with stronger absorbability being well recognized as because of it, Pseudomonas alcaligenes belongs to antibacterial class, its adsorption mechanism is mainly intracellular absorption, first it is the complexation of cell surface, followed by diffusion process slowly inside antibacterial, apply while these two kinds of bacterium, can be under the effect of different adsorption mechanisms, to different heavy metal (Cd2+,Hg2+,Cu2+,Zn2+,Ni2+,Ag2+,Pb2+) it is respectively provided with adsorption well, when adsorbing in immobilized cell ball is filled in bioreactor, effect due to aerator, immobilized cell ball is suspended in the adsorption space that upper screen cloth, lower screen cloth and adsorption column sidewall are surrounded, and form circulation outside annular water conservancy diversion entire body, enhance contacting of immobilized cell ball and waste water, and then strengthen both heavy metal ionic adsorption.
Accompanying drawing explanation
Fig. 1 is the structural representation of biological adsorber.
Detailed description of the invention
Embodiment 1 mixing fermentation culture collects pseudomonas pseudoalcaligenes and saccharomyces cerevisiae thalline, comprises the steps:
1) cultivation of saccharomyces cerevisiae seed liquor
Saccharomyces cerevisiae slant strains is inoculated in primary-seed medium and carries out shake-flask culture, at 30 DEG C, after rotating speed 260rmp cultivates 24h, be inoculated in secondary seed medium and carry out fermentor cultivation, at 30 DEG C, rotating speed 260rmp, ventilation 2.5～3m3/ h obtains the seed liquor of saccharomyces cerevisiae after cultivating 24h, wherein said primary-seed medium is: yeast extract 5g/L, peptone 6g/L, glucose 20g/L；Secondary seed medium is: glucose 40g/L, yeast extract 5g/L, peptone 5g/L, (NH4)2SO42g/L, MgSO4·7H2O 0.2 5g/L, NaCl 0.15g/L, KH2PO4 2.8g/L.
2) cultivation of Pseudomonas alcaligenes seed liquor
Zymomonas mobilis slant strains is inoculated in primary-seed medium and carries out shake-flask culture, at 30 DEG C, after rotating speed 160rmp cultivates 24h, is inoculated in secondary seed medium and carries out fermentor cultivation, at 30 DEG C, and rotating speed 160rmp, ventilation 2.5～3m3/ h cultivates 24h, obtains the seed liquor of saccharomyces cerevisiae, and wherein, described primary-seed medium is: glucose 20g/L, yeast extract 5g/L, peptone 6g/L；Described secondary seed medium is: glucose 20g/L, soybean cake powder 10g/L, KNO31g/L, KH2PO41g/L, FeCl2·6H2O0.05g/L, CaCl2·7H2O0.02g/L, MgSO4·7H2O 1g/L；
3) mixed fermentation
By step 1) the saccharomyces cerevisiae seed liquor of gained and step 2) the Pseudomonas alcaligenes seed liquor 1-3:1-3 by volume of gained mixes, proceed to fermentation medium is carried out High Density Cultivation with the inoculum concentration of 20%, at 30 DEG C, rotating speed 260rmp, ventilation 5～10m3/ h cultivates 36-48h, and the culture fluid after fermenting stands, and centrifugal enrichment thalline is pseudomonas pseudoalcaligenes and saccharomyces cerevisiae thalline, and described fermentation medium is: glucose sugar 80g/L, yeast extract 5g/L, peptone 5g/L, soybean cake powder 10g/L, KNO31g/L, KH2PO42g/L, FeCl2·6H2O 0.05g/L, CaCl2·7H2O 0.02g/L, MgSO4·7H2O 1.5g/L, NaCl 0.15g/L.
The preparation method of 2 one kinds of immobilized cell balls of embodiment, comprises the steps:
A) after polyvinyl alcohol, calcium alginate, acryloyl being mixed with the water ratio of 12:1:4:100 in mass ratio, obtain the polyvinyl alcohol hydrosol, the pseudomonas pseudoalcaligenes collected through embodiment 1 and saccharomyces cerevisiae thalline are added to the polyvinyl alcohol hydrosol, forms mixed solution, wherein, Pseudomonas alcaligenes and the saccharomyces cerevisiae mixed vaccine of 5g weight in wet base are added during the addition of Pseudomonas alcaligenes and saccharomyces cerevisiae thalline meets the polyvinyl alcohol hydrosol of every 100mL；B) mixed solution peristaltic pump is joined containing 4w/v%N '-methylene-bisacrylamide and 1.5w/v%CaSO4Saturated boric acid solution in, form gel ball, 5-10h is cooled down under the conditions of placing-20 DEG C, thawed at room temperature, cooling down 1-2h under the conditions of being placed in-10 DEG C the most again, after thawed at room temperature, it is in 0.3g/L phosphate that gel ball puts into mass concentration, it is spherical for obtaining profile, the internal immobilized cell ball A in porous network structure.
The preparation method of 3 one kinds of immobilized cell balls of embodiment, comprises the steps:
A) after polyvinyl alcohol, calcium alginate, acryloyl being mixed with the water ratio of 6:0.5:3:100 in mass ratio, obtain the polyvinyl alcohol hydrosol, the pseudomonas pseudoalcaligenes collected through embodiment 1 and saccharomyces cerevisiae thalline are added to the polyvinyl alcohol hydrosol, forms mixed solution, and the addition controlling Pseudomonas alcaligenes and saccharomyces cerevisiae thalline meets Pseudomonas alcaligenes and the saccharomyces cerevisiae mixed vaccine adding 4g weight in wet base in the polyvinyl alcohol hydrosol of every 100mL；Again mixed solution peristaltic pump is joined containing 3w/v%N '-methylene-bisacrylamide and 1w/v%CaSO4Saturated boric acid solution in, form gel ball, 5-10h is cooled down under the conditions of placing-20 DEG C, thawed at room temperature, cooling down 1-2h under the conditions of being placed in-10 DEG C the most again, after thawed at room temperature, it is in 0.2g/L phosphate that gel ball puts into mass concentration, it is spherical for obtaining profile, the internal immobilized cell ball B in porous network structure.
The preparation method of 4 one kinds of immobilized cell balls of embodiment, comprises the steps:
A) after polyvinyl alcohol, calcium alginate, acryloyl being mixed with the water ratio of 15:1.5:5:100 in mass ratio, obtain the polyvinyl alcohol hydrosol, the pseudomonas pseudoalcaligenes collected through embodiment 1 and saccharomyces cerevisiae thalline are added to the polyvinyl alcohol hydrosol, forms mixed solution, wherein, Pseudomonas alcaligenes and the saccharomyces cerevisiae mixed vaccine of 6g weight in wet base are added during the addition of Pseudomonas alcaligenes and saccharomyces cerevisiae thalline meets the polyvinyl alcohol hydrosol of every 100mL；B) mixed solution peristaltic pump is joined containing 5w/v%N '-methylene-bisacrylamide and 2w/v%CaSO4Saturated boric acid solution in, form gel ball, 5-10h is cooled down under the conditions of placing-20 DEG C, thawed at room temperature, cooling down 1-2h under the conditions of being placed in-10 DEG C the most again, after thawed at room temperature, it is in 0.5g/L phosphate that gel ball puts into mass concentration, it is spherical for obtaining profile, the internal immobilized cell ball C in porous network structure.
Application Example 2,3 or 4 gained immobilized cell ball carries out the processing method of heavy metal wastewater thereby, comprises the steps:
null1) preparation of biological adsorption device: as shown in Figure 1，Described biological adsorption group is formed by 3 adsorption column 1 concatenations，It is provided with water inlet on adsorption column lower sides，It is provided with overfall in adsorption column upper portion side wall，Upper screen cloth 2 and lower screen cloth 3 it is provided with in adsorption column 1，Upper screen cloth 2 position is higher than the position at water inlet place，Lower screen cloth 3 position is less than the position at overfall place，Described adsorption column sidewall is with upper、Immobilized cell ball is filled in the adsorption space that lower screen cloth is surrounded，Immobilized cell ball is 60% at the total filling rate of adsorption space，Annular guide shell 4 it is additionally provided with in this adsorption space，The top and bottom of described annular guide shell 4 do not contact with upper screen cloth and lower screen cloth respectively，Aerator 5 it is provided with bottom adsorption column，This aerator 5 is positioned at below annular guide shell 4，This aerator makes immobilized cell ball form circulation inside and outside annular stream guiding barrel.
2) process of heavy metal wastewater thereby: by concentration of heavy metal ion adjustment≤800mg/L initial in heavy metal wastewater thereby, after PH is adjusted to 5-7, flow through biological adsorption device successively, the hydraulic detention time 0.5-1h in every adsorption column.
Immobilized cell ball A, immobilized cell ball B, immobilized cell ball C are filled in above-mentioned different bioreactor respectively, are respectively designated as the first biological adsorption device, the second biological adsorption device, the 3rd biological adsorber, the preparation Cu Han 30mg/L respectively2+、Zn2+、Ni2+、Cd2+、Cr2+、Hg2+、Pb2+Mixed solution, adjust after pH=5-7, the concentration change passing in and out each biological adsorption device is as shown in the table:
Above-described embodiment is only for technology design and the feature of the explanation present invention; its object is to allow person skilled in the art will appreciate that present disclosure and to implement according to this; can not limit the scope of the invention with this; all equivalence changes made according to spirit of the invention or modification, all should contain within protection scope of the present invention.
Claims (2)
1. a waste water treating agent, it is characterised in that adopt and be prepared from the following method,
1) mixing fermentation culture and collection pseudomonas pseudoalcaligenes and saccharomyces cerevisiae thalline:
A) cultivation of saccharomyces cerevisiae seed liquor
Saccharomyces cerevisiae strain on inclined-plane is inoculated in primary-seed medium and carries out shake-flask culture, at 27-30 DEG C, after rotating speed 260rmp cultivates 24h, be inoculated in secondary seed medium and carry out fermentor cultivation, at 30-32 DEG C, rotating speed 260-280rmp, ventilation 2.5～3m3/ h obtains the seed liquor of saccharomyces cerevisiae after cultivating 24-36h, wherein said primary-seed medium is: yeast extract 5g/L, peptone 6g/L, glucose 20g/L;Secondary seed medium is: glucose 40-50 g/L, yeast extract 5-8 g/L, peptone 5-8 g/L, (NH4) 2SO4 2-3g/L, MgSO4 7H2O 0.2 5-0.3g/L, NaCl 0.15-0.2 g/L, KH2PO4 2.8-3g/L；
B) cultivation of Pseudomonas alcaligenes seed liquor
Pseudomonas alcaligenes strain on inclined-plane is inoculated in primary-seed medium and carries out shake-flask culture, at 27-30 DEG C, after rotating speed 160-180rmp cultivates 24h, it is inoculated in secondary seed medium and carries out fermentor cultivation, at 27-30 DEG C, rotating speed 160-180rmp, ventilation 2.5～3m3/h is cultivated 24-36h, is obtained the seed liquor of Pseudomonas alcaligenes, wherein, described primary-seed medium is: glucose 20-30g/L, yeast extract 5-8g/L, peptone 6-8g/L；Described secondary seed medium is: glucose 20-30g/L, soybean cake powder 10-12g/L, KNO3 1-1.2g/L, KH2PO4 1-1.2g/L, FeCl2·6H2O 0.05-0.08g/L,CaCl2·7H2O 0.02-0.04g/L, MgSO4·7H2O 1-1.2g/L；
C) mixed fermentation
By saccharomyces cerevisiae seed liquor and the step 2 of step 1) gained) the Pseudomonas alcaligenes seed liquor 1-3:1-3 by volume of gained mixes, proceed to fermentation medium is carried out High Density Cultivation with the inoculum concentration of 10-20%, at 30 DEG C, rotating speed 220-260rmp, ventilation 5～10m3/ h cultivates 36-48h, and the culture fluid after fermenting stands, and centrifugal enrichment thalline is pseudomonas pseudoalcaligenes and saccharomyces cerevisiae thalline, wherein, described fermentation medium is: glucose sugar 80-100g/L, yeast extract 5-10g/L, peptone 5-10g/L, soybean cake powder 10-12g/L, KNO3 1-1.2g/L, KH2PO4 2-2.5g/L, FeCl2·6H2O 0.05-0.08g/L, CaCl2·7H2O 0.02-0.04g/L, MgSO4·7H2O 1.5-1.8g/L, NaCl 0.15-0.18g/L；
2) preparation of immobilized cell ball: A) polyvinyl alcohol, calcium alginate, acryloyl are mixed with water in mass ratio 6～15:0.5～1.5:3～5:100 ratio after, obtain the polyvinyl alcohol hydrosol, the pseudomonas pseudoalcaligenes collected and saccharomyces cerevisiae thalline are added to the polyvinyl alcohol hydrosol, forms mixed solution, wherein, Pseudomonas alcaligenes and the saccharomyces cerevisiae mixed vaccine of 4-6g weight in wet base are added during the addition of Pseudomonas alcaligenes and saccharomyces cerevisiae thalline meets the polyvinyl alcohol hydrosol of every 100mL；B) mixed solution peristaltic pump is joined containing 3～5w/v%N '-methylene-bisacrylamide and 1-2 w/v%CaSO4Saturated boric acid solution in, form gel ball, 5-10h is cooled down under the conditions of placing-20 DEG C, thawed at room temperature, cooling down 1-2h under the conditions of being placed in-10 DEG C the most again, after thawed at room temperature, it is in 0.2～0.5g/L phosphate that gel ball puts into mass concentration, it is spherical for obtaining profile, the internal immobilized cell ball in porous network structure.
2. application waste water treating agent described in claim 1 process heavy metal wastewater thereby method, it is characterised in that comprise the steps:
1) preparation of biological adsorption device: described biological adsorption device is formed by many adsorption column concatenations, it is provided with water inlet on described adsorption column lower sides, it is provided with overfall in adsorption column upper portion side wall, upper screen cloth and lower screen cloth it is provided with in adsorption column, upper screen cloth position is higher than the position at water inlet place, lower screen cloth position is less than the position at overfall place, described adsorption column sidewall is with upper, immobilized cell ball it is filled with in the adsorption space that lower screen cloth is surrounded, immobilized cell ball is 50-80% at the total filling rate of adsorption space, also set up in this adsorption space and be provided with annular guide shell, the top and bottom of described annular guide shell do not contact with upper screen cloth and lower screen cloth respectively, aerator it is provided with bottom adsorption column, this aerator is positioned at below annular guide shell；
2) process of waste water: by concentration of heavy metal ion adjustment≤800mg/L initial in heavy metal wastewater thereby, after PH is adjusted to 5-7, flow through biological adsorption device successively, the hydraulic detention time 0.5-1h in every adsorption column.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106045061A (en) * | 2016-08-03 | 2016-10-26 | 王维娜 | Composite resin adsorbent for lead pollution in river sewage and preparation method of adsorbent |
| CN106040199A (en) * | 2016-08-03 | 2016-10-26 | 王维娜 | Composite resin adsorbent for arsenic pollution in river sewage and preparation method of adsorbent |
| CN106045062A (en) * | 2016-08-03 | 2016-10-26 | 王维娜 | Composite resin adsorbent for manganese pollution in municipal sewage and preparation method of adsorbent |
| CN106040200A (en) * | 2016-08-03 | 2016-10-26 | 王维娜 | Composite resin adsorbent for copper pollution in municipal sewage and preparation method of adsorbent |
| CN106111094A (en) * | 2016-08-03 | 2016-11-16 | 王维娜 | Iron pollution composite resin adsorbent and preparation method thereof in a kind of steel and iron industry sewage |
| CN106179270A (en) * | 2016-08-03 | 2016-12-07 | 王维娜 | Zinc pollution composite resin adsorbent and preparation method thereof in a kind of municipal sewage |
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| CN107446912A (en) * | 2017-08-16 | 2017-12-08 | 辽宁大学 | A kind of immobilized brewing yeast engineering bacteria and its remove heavy metal in waste water Pb2+In application |
| CN111013383A (en) * | 2019-12-19 | 2020-04-17 | 天津农学院 | Malodor inhibitor for microalgae anaerobic biological fermentation and preparation method thereof |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5290693A (en) * | 1992-07-08 | 1994-03-01 | National Science Council | Immobilization of microorganisms or enzymes in polyvinyl alcohol beads |
| CN102174419A (en) * | 2011-03-24 | 2011-09-07 | 南京工业大学 | Rhizopus oryzae strain, mutation screening method thereof and method for producing fumaric acid through fermentation |
| CN102583906A (en) * | 2012-03-21 | 2012-07-18 | 徐州工程学院 | Processing device and processing method of nitrogen pollutant in secondary tail water of sewage treatment plant |
-
2016
- 2016-02-29 CN CN201610112186.9A patent/CN105734037B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5290693A (en) * | 1992-07-08 | 1994-03-01 | National Science Council | Immobilization of microorganisms or enzymes in polyvinyl alcohol beads |
| CN102174419A (en) * | 2011-03-24 | 2011-09-07 | 南京工业大学 | Rhizopus oryzae strain, mutation screening method thereof and method for producing fumaric acid through fermentation |
| CN102583906A (en) * | 2012-03-21 | 2012-07-18 | 徐州工程学院 | Processing device and processing method of nitrogen pollutant in secondary tail water of sewage treatment plant |
Non-Patent Citations (2)
| Title |
|---|
| 李艳红等: "《水污染控制特色特殊实验项目汇编》", 31 May 2012, 中国环境科学出版社 * |
| 王国倩等: "米根霉菌吸附法去除电镀废水中的镍", 《工业用水与废水》 * |
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| CN106179270A (en) * | 2016-08-03 | 2016-12-07 | 王维娜 | Zinc pollution composite resin adsorbent and preparation method thereof in a kind of municipal sewage |
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