CN106491704A - A kind of preparation method of Folium Perillae injection - Google Patents

A kind of preparation method of Folium Perillae injection Download PDF

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Publication number
CN106491704A
CN106491704A CN201611162610.7A CN201611162610A CN106491704A CN 106491704 A CN106491704 A CN 106491704A CN 201611162610 A CN201611162610 A CN 201611162610A CN 106491704 A CN106491704 A CN 106491704A
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folium perillae
injection
preparation
extract
extractum
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郝青
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Shaanxi Yuhang Electronic Co Ltd
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Shaanxi Yuhang Electronic Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
    • A61K36/535—Perilla (beefsteak plant)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/02—Inorganic compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
    • A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
    • A61K47/10—Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0019—Injectable compositions; Intramuscular, intravenous, arterial, subcutaneous administration; Compositions to be administered through the skin in an invasive manner
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/08—Solutions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/55—Liquid-liquid separation; Phase separation

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Engineering & Computer Science (AREA)
  • Inorganic Chemistry (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Medical Informatics (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Dermatology (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

The present invention relates to a kind of preparation method of Folium Perillae injection, it is characterised in that comprise the following steps：Take Folium Perillae and be put into 35 DEG C of drying in baking oven, preliminary crushing is carried out with crusher for Chinese herbal medicine, finally cross 40 mesh sieves and obtain coarse powder.Folium Perillae injection prepared by the method for the invention, safety are good, stimulate, and the method for the invention reduces energy consumption, saves cost, can be practical, can industrialized production.

Description

A kind of preparation method of Folium Perillae injection
Technical field
The present invention relates to a kind of preparation method of Folium Perillae injection.
Background technology
Foot and mouth disease is the artiodactyls disease caused by foot and mouth disease viruses.Foot and mouth disease is the most heavy of impact animal husbandry development The zoonosis that wants, are classified as the No.1 infectious disease in A class infectious disease in OIE, and in China, the disease is also arranged For a class animal epidemic.The outburst of foot and mouth disease can cause serious loss to local animal husbandry.Calendar year 2001 Britain's outburst foot and mouth disease Thin Sui, slaughters 7,000,000 domestic animals, causes 8,500,000,000 pounds of loss, meanwhile, the epidemic situation also involves France, Holland, Ireland etc. State, these countries slaughter up to 360,000 domestic animals altogether.In developing country, injection foot-and-mouth disease vaccine is that prevention foot and mouth disease is most effective One of measure.But as foot and mouth disease viruses are RNA viruses, quickly, therefore the antigenic characteristic of the gene of virus and virus changes, This dramatically increases the difficulty for developing efficient foot-and-mouth disease vaccine.Although developed country is slaughtered using animal can effectively prevent El Fever aphthous, but once outburst foot and mouth disease, does not have immune animal to face bigger infection risk mouth in a large number.Therefore, foot and mouth disease is anti- Control is still animal epidemic disease control in animal husbandry and studies one of most important research topic.It is also prevention mouth using effective health measure One of technical measures of fever aphthous.Foot and mouth disease viruses be one kind to sour extremely sensitive virus.This is because foot and mouth disease viruses are in acid Protein coat is taken off under the conditions of property to be bred；If it does not also infect entrance zooblast, taking off protein coat in advance will The breeding of virus can be affected and cause the death of virus.Chinese herbal medicine red tea funguss be a kind of using red tea funguss fermented tea, Fructus Momordicae, The black tea mattress bacterium solution of the extracts of Chinese herbal medicine such as Radix Glycyrrhizae, Flos Chrysanthemi Indici.As it remains the characteristic of lactic acid production of red tea funguss, therefore in Medical herbs black tea bacteria preparation can effectively kill foot and mouth disease viruses in vitro.Folium Camelliae sinensis, Fructus Momordicae, Radix Glycyrrhizae, Flos Chrysanthemi Indici are that some have medicine The dual-purpose Chinese herbal medicine of food, the extracting solution of these plants generally have raising animal immunizing power, antagonism pathogenetic bacteria, virus etc. The effect of aspect.
Content of the invention
The present invention proposes a kind of preparation method of Folium Perillae injection.
A kind of preparation method of Folium Perillae injection, comprises the following steps：
（1）Take Folium Perillae and be put into 35 DEG C of drying in baking oven, preliminary crushing is carried out with crusher for Chinese herbal medicine, finally cross 40 mesh sieves and obtain Coarse powder；
(2) accurately weigh Folium Perillae leaf coarse powder 400g to be placed in round-bottomed flask, compare 1 by material liquid：12 (W/V), hot water at 100 DEG C Extraction 4 times, each 1h, every time between carry out coarse filtration with gauze, united extraction liquid, rotation are evaporated excessive moisture, obtain final product Folium Perillae Extractum；
(3) 60% ethanol solution is added in the Folium Perillae extractum for preparing, and stands 24h；
（4）Filtered with filter paper after stable precipitation to be precipitated, partial impurities are removed using water extract-alcohol precipitation principle, and use Rotary Evaporators Reclaim the ethanol in solution；
（5）Salt acid for adjusting pH value with 18% to 2.0～2.5, according to solid-liquid ratio 5：3 ratio, adds ethyl acetate repeatedly to extract Take, till ethyl acetate layer solution is colourless；
（6）Combining extraction liquid, rotation are evaporated ethyl acetate, obtain final product Folium Perillae rosmarinic acid extract；
（7）0.9% Sodium Chloride Injection 360ml dissolving extractum is added in the Folium Perillae rosmarinic acid extract for preparing, and Sodium sulfite 0.1%, phenol 0.25% are simultaneously introduced respectively as antioxidant and antibacterial, are filtered in 0.45 micron of micropore Film sucking filtration, with salt acid for adjusting pH value to 6.0；
（8）L000ml is settled to water for injection, fully vibration is mixed, aseptic subpackaged, moist heat sterilization under 121 DEG C of environment 30min, after obtain final product, sealing wax keeps in dark place.
Preferably, the step step（5）Middle pH value is adjusted to 2.5.
Folium Perillae injection prepared by the method for the invention, safety are good, stimulate, and the method for the invention is reduced Energy consumption, saves cost, can be practical, can industrialized production.
Specific embodiment
Embodiment 1.
A kind of preparation method of Folium Perillae injection, comprises the following steps：
（1）Take Folium Perillae and be put into 35 DEG C of drying in baking oven, preliminary crushing is carried out with crusher for Chinese herbal medicine, finally cross 40 mesh sieves and obtain Coarse powder；
(2) accurately weigh Folium Perillae leaf coarse powder 400g to be placed in round-bottomed flask, compare 1 by material liquid：12 (W/V), hot water at 100 DEG C Extraction 4 times, each 1h, every time between carry out coarse filtration with gauze, united extraction liquid, rotation are evaporated excessive moisture, obtain final product Folium Perillae Extractum；
(3) 60% ethanol solution is added in the Folium Perillae extractum for preparing, and stands 24h；
（4）Filtered with filter paper after stable precipitation to be precipitated, partial impurities are removed using water extract-alcohol precipitation principle, and use Rotary Evaporators Reclaim the ethanol in solution；
（5）Salt acid for adjusting pH value with 18% to 2.0～2.5, according to solid-liquid ratio 5：3 ratio, adds ethyl acetate repeatedly to extract Take, till ethyl acetate layer solution is colourless；
（6）Combining extraction liquid, rotation are evaporated ethyl acetate, obtain final product Folium Perillae rosmarinic acid extract；
（7）0.9% Sodium Chloride Injection 360ml dissolving extractum is added in the Folium Perillae rosmarinic acid extract for preparing, and Sodium sulfite 0.1%, phenol 0.25% are simultaneously introduced respectively as antioxidant and antibacterial, are filtered in 0.45 micron of micropore Film sucking filtration, with salt acid for adjusting pH value to 6.0；
（8）L000ml is settled to water for injection, fully vibration is mixed, aseptic subpackaged, moist heat sterilization under 121 DEG C of environment 30min, after obtain final product, sealing wax keeps in dark place.
Embodiment 2.
A kind of preparation method of Folium Perillae injection, comprises the following steps：
（1）Take Folium Perillae and be put into 35 DEG C of drying in baking oven, preliminary crushing is carried out with crusher for Chinese herbal medicine, finally cross 40 mesh sieves and obtain Coarse powder；
(2) accurately weigh Folium Perillae leaf coarse powder 400g to be placed in round-bottomed flask, compare 1 by material liquid：12 (W/V), hot water at 100 DEG C Extraction 4 times, each 1h, every time between carry out coarse filtration with gauze, united extraction liquid, rotation are evaporated excessive moisture, obtain final product Folium Perillae Extractum；
(3) 60% ethanol solution is added in the Folium Perillae extractum for preparing, and stands 24h；
（4）Filtered with filter paper after stable precipitation to be precipitated, partial impurities are removed using water extract-alcohol precipitation principle, and use Rotary Evaporators Reclaim the ethanol in solution；
（5）Salt acid for adjusting pH value with 18% to 2.0～2.5, according to solid-liquid ratio 5：3 ratio, adds ethyl acetate repeatedly to extract Take, till ethyl acetate layer solution is colourless；
（6）Combining extraction liquid, rotation are evaporated ethyl acetate, obtain final product Folium Perillae rosmarinic acid extract；
（7）0.9% Sodium Chloride Injection 360ml dissolving extractum is added in the Folium Perillae rosmarinic acid extract for preparing, and Sodium sulfite 0.1%, phenol 0.25% are simultaneously introduced respectively as antioxidant and antibacterial, are filtered in 0.45 micron of micropore Film sucking filtration, with salt acid for adjusting pH value to 6.0；
（8）L000ml is settled to water for injection, fully vibration is mixed, aseptic subpackaged, moist heat sterilization under 121 DEG C of environment 30min, after obtain final product, sealing wax keeps in dark place.
Step step described in the present embodiment（5）Middle pH value is adjusted to 2.5.

Claims (2)

1. a kind of preparation method of Folium Perillae injection, it is characterised in that comprise the following steps：
（1）Take Folium Perillae and be put into 35 DEG C of drying in baking oven, preliminary crushing is carried out with crusher for Chinese herbal medicine, finally cross 40 mesh sieves and obtain Coarse powder；
(2) accurately weigh Folium Perillae leaf coarse powder 400g to be placed in round-bottomed flask, compare 1 by material liquid：12 (W/V), hot water at 100 DEG C Extraction 4 times, each 1h, every time between carry out coarse filtration with gauze, united extraction liquid, rotation are evaporated excessive moisture, obtain final product Folium Perillae Extractum；
(3) 60% ethanol solution is added in the Folium Perillae extractum for preparing, and stands 24h；
（4）Filtered with filter paper after stable precipitation to be precipitated, partial impurities are removed using water extract-alcohol precipitation principle, and use Rotary Evaporators Reclaim the ethanol in solution；
（5）Salt acid for adjusting pH value with 18% to 2.0～2.5, according to solid-liquid ratio 5：3 ratio, adds ethyl acetate repeatedly to extract Take, till ethyl acetate layer solution is colourless；
（6）Combining extraction liquid, rotation are evaporated ethyl acetate, obtain final product Folium Perillae rosmarinic acid extract；
（7）0.9% Sodium Chloride Injection 360ml dissolving extractum is added in the Folium Perillae rosmarinic acid extract for preparing, and Sodium sulfite 0.1%, phenol 0.25% are simultaneously introduced respectively as antioxidant and antibacterial, are filtered in 0.45 micron of micropore Film sucking filtration, with salt acid for adjusting pH value to 6.0；
（8）L000ml is settled to water for injection, fully vibration is mixed, aseptic subpackaged, moist heat sterilization under 121 DEG C of environment 30min, after obtain final product, sealing wax keeps in dark place.
2. a kind of preparation method of Folium Perillae injection as claimed in claim 1, it is characterised in that the step step（5）In PH value is adjusted to 2.5.
CN201611162610.7A 2016-12-15 2016-12-15 A kind of preparation method of Folium Perillae injection Pending CN106491704A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
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Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201611162610.7A CN106491704A (en) 2016-12-15 2016-12-15 A kind of preparation method of Folium Perillae injection

Publications (1)

Publication Number Publication Date
CN106491704A true CN106491704A (en) 2017-03-15

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Application publication date: 20170315

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