CN103919839B - The application of Snakegourd Fruit, safflower and Ligusticum wallichii in drosophila enteron aisle oxidativestress damage is alleviated - Google Patents
The application of Snakegourd Fruit, safflower and Ligusticum wallichii in drosophila enteron aisle oxidativestress damage is alleviated Download PDFInfo
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- CN103919839B CN103919839B CN201410155605.8A CN201410155605A CN103919839B CN 103919839 B CN103919839 B CN 103919839B CN 201410155605 A CN201410155605 A CN 201410155605A CN 103919839 B CN103919839 B CN 103919839B
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- drosophila
- safflower
- snakegourd fruit
- enteron aisle
- rhizoma chuanxiong
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Abstract
The present invention is the application of Snakegourd Fruit, safflower and Rhizoma Chuanxiong extract in drosophila enteron aisle oxidative damage is alleviated.Snakegourd Fruit, safflower and Rhizoma Chuanxiong extract are obtained using water extraction, drosophila enteron aisle model of oxidative reparative experiment is carried out to Snakegourd Fruit, safflower and Rhizoma Chuanxiong extract, as a result find that Snakegourd Fruit, safflower and Rhizoma Chuanxiong extract have good anti-oxidant, removing free radical isoreactivity, available for preparing anti-oxidation medicine, the medicine of especially anti-enteron aisle oxidative damage.
Description
Technical field
The present invention relates to the application of Snakegourd Fruit, safflower and Rhizoma Chuanxiong extract in terms of drosophila enteron aisle oxidative damage is alleviated, it is related to
Technical field of innate immunity of drosophila melanogaster.
Background technology
Enteron aisle not only has digestion and absorption function, and is the maximum immune organ of human body.When pathogenic microorganism invades intestines
During road, enteron aisle inherent immunity System Take local defense effect.Enteron aisle inherent immunity system mainly includes intestinal mucosa epithelium screen
Barrier, inherent immunity cell, inherent immunity molecule etc..Wherein function of intestinal mucosa barrier in patient is a key character of enteron aisle, works as the external world
Environment, stress, the factor such as infection can break balance between the normal flora in intestinal mucosal barrier and host when stimulating enteron aisle, make
Lacked of proper care into enteron aisle homeostasis.
The signal transduction pathway that drosophila participates in as important model organism, its enteron aisle structure, cellularity and stem cell
There are many similarities with mammal.Therefore research drosophila intestine immunity regulation mechanism and intestinal stem cell differentiation mechanism are to taking off
Showing the pathogenesis of human intestine's relevant disease has important directive function.Drosophila adult intestinal tissue homeostasis depends on
The removing of dead cell and the generation of neoblast, and the intestinal stem cell (ISC) with cell division capacity is in tissue homeostasis
Maintenance in terms of serve it is most important.One intestinal stem cell, which carries out Asymmetric division, can produce two cells, one of them
For cell to keep the ISC of stem cell properties, another is of short duration nondividing enteroblast (enteroblast, EB), thin into intestines
Born of the same parents can continue to be divided into the enterocyte (enterocyte, EC) of absorption-type or the endocrine cell of secreting type
(enteroendocrine cell,EE).The maintenance of intestinal tissue and renewal are realized by the collective effect of these cells.So
And when stem cell population changes or can cause organ-system failure during function reduction, induce canceration and organism aging process.
Snakegourd Fruit (Trichosanthes kirilowii Maxim.) has clearing away heat and eliminating phlegm, relieving stuffiness of the chest by dispersing aggregation of pathogens, moisturize laxation etc.
Effect, for treating the illnesss such as cough with lung heat, the turbid thick, chest impediment and cardialgia of Huang of phlegm.Mainly containing volatile oil, grease type, sterols, Chinese juniper
The compositions such as building ester alkali, amino acid, there is antibacterial, anticancer, improve hypoxia-bearing capability, reduce a variety of effects such as serum cholesterol.It is red
Flower (Carthamus tinctorius L.) is traditional medicine, has the effect of invigorate blood circulation, blood stasis removing analgesic, is followed for treating
Ring disorder disease.Its active ingredient is mainly carthamin yellow and safflower red pigment.Wherein, carthamin yellow, which has, removes hydroxyl certainly
By base, anti-lipid peroxidation, protect nerve from the effect such as damage.Ligusticum wallichii (Ligusticum chuanxiong Hort.)
The effect of with promoting blood circulation and removing blood stasis, wind-expelling pain-stopping, for illnesss such as treating swelling and pain by traumatic injury, headache, arthralgia pain due to rheumatism.Mainly containing volatile oil, life
The composition such as alkaloids, phenols, polysaccharide and organic acid, phthalide lactone, there is analgesia, antitumor, antibacterial and remove free radical in vitro
Deng effect.However, there has been no the relevant report for being used to alleviate enteron aisle oxidative damage to Snakegourd Fruit, safflower and Rhizoma Chuanxiong extract at present.
The content of the invention
The purpose of the present invention is the application for disclosing Snakegourd Fruit, safflower and Rhizoma Chuanxiong extract in enteron aisle oxidative damage is alleviated.
The above-mentioned purpose of the present invention is achieved by following scheme：
Drosophila feeding oxidative stress thing (Paraquat and H are given first2O2) establish drosophila enteron aisle model of oxidative, will
Snakegourd Fruit, safflower and Rhizoma Chuanxiong extract carry out drosophila survival rate and the experiment such as intestinal stem cell and enteroblast quantitative observation, secondly
To drosophila difference feeding Snakegourd Fruit, safflower and Rhizoma Chuanxiong extract, life span of drosophila melanogaster and aging drosophila intestinal stem cell are observed and into intestines
Cell quantity changes.As a result find that Snakegourd Fruit, safflower and Rhizoma Chuanxiong extract have anti-oxidant, removing free radical isoreactivity well,
Available for anti-oxidation medicine is prepared, the medicine of enteron aisle oxidative damage is especially treated.
Brief description of the drawings
Fig. 1 is Snakegourd Fruit, safflower and Rhizoma Chuanxiong extract to Paraquat and H2O2The influence of drosophila survival rate after processing；
Fig. 2 be Snakegourd Fruit, safflower and Rhizoma Chuanxiong extract to Paraquat processing after drosophila enterocyte immunostaining results and
Enteron aisle is dry thin
Born of the same parents and the influence of enteroblast quantity；
Fig. 3 is the influence of Snakegourd Fruit, safflower and Rhizoma Chuanxiong extract to life span of drosophila melanogaster；
Fig. 4 is that Snakegourd Fruit, safflower and Rhizoma Chuanxiong extract are done to the enterocyte immunostaining results and enteron aisle of 30day aging drosophilas
Cell and
The influence of enteroblast quantity；
Embodiment
With reference to example, the invention will be further described：
Experimental animal：Wild type W1118Strain Drosophila melanogaster (Drosophila melanogaster) preserves for laboratory,
EsgGal4UAS-GFP attacks professor Rong Wen by Peking University and provided.Drosophila cultivation temperature is 25 DEG C, relative humidity 60%.
The preparation of Drosophila medium：Control group culture medium prescription is corn flour 14.2g, agar 1.1g, dusty yeast 3.3g, big
Bean powder 1.9g, white sugar 17g, propionic acid 0.94mL, deionized water 200mL.Added in experimental group isometric with control group deionized water
Snakegourd Fruit, safflower and Ligusticum wallichii extract solution, the concentration of Snakegourd Fruit, safflower and Rhizoma Chuanxiong extract is 10% in experimental group.
Each experiment reagent that the present embodiment is related to is that commercially available analysis is pure：Paraquat and H2O2Deng.
The preparation of the Snakegourd Fruit of embodiment 1, safflower and Ligusticum wallichii extract solution
Snakegourd Fruit, safflower and Ligusticum wallichii respectively weigh 20g, add 400mL deionized waters immersion 12h, and moderate heat boils 3h, filtered, filter
Slag adds 200mL deionized waters and extracted 1 time with same method, merges 2 filtrates and is concentrated into 100mL, that is, it is dense to obtain quality
Spend for 0.2g/mL (20%) Chinese herbal medicine Aqueous extracts, put -40 DEG C and save backup.
The Snakegourd Fruit of embodiment 2, safflower and Rhizoma Chuanxiong extract are to drosophila intestinal stem cell and enteroblast after Paraquat processing
The influence of quantity
With the drosophila cultivated in ordinary culture medium as a control group, the drosophila cultivated in Snakegourd Fruit, safflower and Ligusticum wallichii culture medium
As experimental group.Random collecting control group and experimental group have been sprouted wings 3~5d drosophila respectively, female each 15 of male drosophila in every group.
To being transferred to after each group drosophila Nature enemy 2h in the drosophila pipe for putting the filter paper that is of five storeys, into filter paper add 6mM paraquat or
1%H2O2Solution (contains 5% sucrose), is sufficiently humidified so as to filter paper, and a filter paper is changed per 24h and records drosophila death toll.
As a result show：After paraquat processing drosophila 6d, control group survival rate drops to 1.1%, and feeding Snakegourd Fruit, safflower and
The experimental group survival rate of Ligusticum wallichii is respectively 75.5% (* * * P<0.001), 95.6% (* * * P<And 67.7% (* * P 0.001)<
0.01) 74.4%, 94.5% and 66.6% (Figure 1A), has been respectively increased compared with control group.In addition, H2O2After handling drosophila 6d,
The experimental group survival rate of feeding Snakegourd Fruit, safflower and Ligusticum wallichii is respectively 41.1% (* * P<0.01), 50% (* * P<0.01) and
54.4% (* * * P<0.001), it is 2.4,2.99 and 3.2 times (Figure 1B) of control group (16.7%).The above results explanation, Snakegourd Fruit,
Safflower and Rhizoma Chuanxiong extract can alleviate paraquat and H2O2The oxidativestress damage of induction, considerably improve the existence of drosophila
Rate.
The enterocyte immunostaining results of the Snakegourd Fruit of embodiment 3, safflower and Rhizoma Chuanxiong extract to drosophila after Paraquat processing
With the influence of intestinal stem cell cell and enteroblast quantity
Paraquat (10mM) of random collecting control group and experimental group emergence the 4-5d female drosophila feeding containing 5% sucrose is molten
Liquid 16h, 10-12 bar drosophila enteron aisles are extracted under microscope in paraformaldehyde, normal temperature fixes 30min, DAPI dyeing 10min, sweet
Fluorescence microscopy Microscopic observation and taken pictures after sheet for oil seal.
As a result show：GFP positive cells (do by precursor, including enteron aisle in the experimental group of feeding Snakegourd Fruit, safflower and Ligusticum wallichii
Cell and enteroblast) quantity is substantially less than control group, reduce 47.1%, 38.6%, 31.3% respectively compared with control group
(***P<0.001).As a result Fig. 2 is seen.
The Snakegourd Fruit of embodiment 4, the influence of safflower and Rhizoma Chuanxiong extract to life span of drosophila melanogaster
The drosophila of random collecting control group and experimental group emergence 4d.Male and female separate, every group 30.Change per 2d and once cultivate
Base, daily timing count drosophila death toll, until all dead.
As a result show：Compared with control group, Fructus Trichosanthis extract can extend female, the male drosophila life-span；Safflower or Ligusticum wallichii extraction
Thing only extends female life span of drosophila melanogaster and not notable to male drosophila aging effects, as a result sees Fig. 3.
The enterocyte immunostaining results and enteron aisle of the Snakegourd Fruit of embodiment 5, safflower and Rhizoma Chuanxiong extract to 30day aging drosophilas
Stem cell and the influence of enteroblast quantity
After random collecting control group and experimental group emergence 4d female drosophila culture 30d, 10-12 bar drosophilas are extracted under microscope
For enteron aisle in paraformaldehyde, normal temperature fixes 30min, DAPI dyeing 10min, fluorescence microscopy Microscopic observation and claps after glycerine mounting
According to.
As a result show：GFP positive cells (do by precursor, including enteron aisle in the experimental group of feeding Snakegourd Fruit, safflower and Ligusticum wallichii
Cell and enteroblast) quantity is substantially less than control group, reduce 30.2%, 28.6%, 41.9% respectively compared with control group
(***P<0.001).As a result Fig. 4 is seen.
In summary, the survival rate to drosophila, the life-span of drosophila, intestinal stem cell and enteroblast number change are passed through
Analysis, it is believed that Snakegourd Fruit, safflower and Rhizoma Chuanxiong extract can alleviate drosophila enteron aisle oxidative damage.
Claims (1)
1. purposes of the Snakegourd Fruit water extract in the medicine for alleviating drosophila enteron aisle oxidative damage is prepared, it is characterised in that
Snakegourd Fruit water extract improves the survival rate after drosophila oxidative damage, reduces drosophila oxidative damage rear intestinal stem cell and thin into intestines
Born of the same parents' quantity.
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| CN201410155605.8A CN103919839B (en) | 2014-04-18 | 2014-04-18 | The application of Snakegourd Fruit, safflower and Ligusticum wallichii in drosophila enteron aisle oxidativestress damage is alleviated |
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| CN201410155605.8A CN103919839B (en) | 2014-04-18 | 2014-04-18 | The application of Snakegourd Fruit, safflower and Ligusticum wallichii in drosophila enteron aisle oxidativestress damage is alleviated |
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| CN105106476B (en) * | 2015-09-28 | 2020-02-07 | 东北林业大学 | Application of saffron crocus in relieving fruit fly intestinal inflammation injury |
| CN107865423A (en) * | 2017-11-10 | 2018-04-03 | 大连大学 | A kind of SOD oral liquids of anti-enteron aisle oxidation |
| CN110604823B (en) * | 2019-10-21 | 2020-10-20 | 兰州大学 | Method for rapidly screening anti-saccharification and/or anti-aging substances |
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