CN106490589A - Active Oviductus Ranae selenium-enriched protein standard solid beverage - Google Patents

Abstract

Active Oviductus Ranae selenium-enriched protein standard solid beverage activity refers to that from the lower production line time is put to death be 2h.The present invention obtains forest frog fat beverage through inventor with expert group's examination and can reach described in state-promulgated pharmacopoeia, and Oviductus Ranae has the effect of the kidney invigorating and essence nourishing, yin nourishing and grows lung, the key of strengthening by means of tonics to be that pressure is quick-fried make albumen all aminoacid.Temperature determines the characteristic that " oil " can be played, pH value also positive correlation.Technological process：Take the sterilization of Changbai Mountain selenium-rich wood frog and put to death → peel off fallopian tube → enter extra-high tension unit, press quick-fried cell wall → colloid defibrination → enzymolysis → filtrations → fine straining → bacterium examine → automatically lyophilizing canned → fractional pack → pack greatly → dispatch from the factory.

Description

Active Oviductus Ranae selenium-enriched protein standard solid beverage

Technical field：

The present invention is Chinese medicine science and technology bioengineering field, for health care, medical treatment.

Background technology：

The institute of agricultural sciences of the Chinese Academy of Sciences with Ganoderma as converting plant rich in selenium albumen-" health must selenium ", this patent with wood frog for turn Change animal Oviductus Ranae selenium-enriched protein, bioconversion design standard is 17 μ g ‰, and Oviductus Ranae itself has 0.003% Selenium content.

Content of the invention：

This patent be forest frog culture base wood frog is carried out sodium selenite, selenium dioxide, dichloride selenium, four Selenium monochloride. is thrown and raises wood frog, and first time bioconversion is to throw to raise conventional feed 99.99% plus 470 μ g% of selenium compound Bioconversion is carried out to tenebrio molitor, Lumbricuss, second bioconversion is to throw to raise first time feed conversion, through two Year selenium-enriched protein wood frog trunk, Se content is up to 17 μ g ‰.And the trunk for being not up to design standard is surpassed " sodium selenite ", " selenium dioxide ", " dichloride selenium ", " selenic chloride " of the compulsory equivalent of high pressure equipment, Equivalent pressure, equivalent temperature to the selenizing conjunction of equivalent, complexation and are reached bioconversion and are equally imitated by design requirement Really.Equivalent used is that different batch wood frogs have different selenium contents.Selenium compound according to different selenium content sovolin amounts Extra-high tension unit selenizing is used, as shown in technological process.

Technological process：

Sterilized selenium-rich wood frog → segmentation is put to death in GMP workshops fallopian tube → wood frog oviduct is peeled off naturally containing selenium Amount 0.003%, is surpassing not up to design requirement sovolin amount sodium selenite is pressed during 17 μ ‰ selenium proteins of g through bioconversion 450Mpa high pressure, time 20min cell pressure is selected between high pressure equipment effective pressure 550Mpa-150Mpa Explosion wall, sterilizing, pressure release to 150Mpa be detained 5min go out mould, extra-high tension unit hip temperature be 18 DEG C, the sulfur of methionine cystine in albumen is replaced into selenium by sodium selenite, in the visible selenizing of amino-acid analyzer Methionine, selenizing cystine, pressure release have light sulfur taste effusion, conversion biology with cultivation wood frog feeding selenium It is as good as, pH value slightly rises between 4.8～6.2 → with 4.8%Nacl sterilized water 80%, material 20% is thrown Enter medical gels mill defibrination, standard is 125 μm of diameter after dry materials → must go up through cloth-bag type centrifugal filtration machine filter Clearly → and enter enzymatic vessel with 36 DEG C of temperature, pH value 6, with bromelain 0.3%, digest 2h → filtration → essence Filter → enter the canned streamline of full-automatic lyophilizing, with every bottle of 0.5g canned → fill per 5 bottles of subpackages, with 5 bottles of 10ml Water and drinking takes out sampling observation antibacterial before 1 → six subpackage of dress 10ml syringes fill → is packed greatly → dispatched from the factory → dispatch from the factory.

Claims (1)

1. all use extra-high tension unit isostatic cool pressing, high temperature insostatic pressing (HIP) effective pressure, temperature, pH value, enzyme values.

Specific embodiment：

Example one：

Wood frog oviduct → be dissolved in inorganic salt with alkali formula selenium dioxide is put to death → is taken in wood frog sterilization, in alkali and separate out colloid be re-dissolved in wood frog aminoacid → lyophilization or 48 DEG C of freeze-day with constant temperature or 48 DEG C of forced ventilation drying → fallopian tube crush → beat piece or bottling → Co 60 sterilizing → bacterium examine → pack → dispatch from the factory.

Example two：

Wood frog put to death → take Oviductus Ranae → freeze-day with constant temperature → micronizing → stirring selenium compound → Co 60 sterilize → bottle → dispatch from the factory.