CN106489843A - A kind of method that can judge Mauremys mutica embryonic development phase without the need for dissection - Google Patents
A kind of method that can judge Mauremys mutica embryonic development phase without the need for dissection Download PDFInfo
- Publication number
- CN106489843A CN106489843A CN201611002375.7A CN201611002375A CN106489843A CN 106489843 A CN106489843 A CN 106489843A CN 201611002375 A CN201611002375 A CN 201611002375A CN 106489843 A CN106489843 A CN 106489843A
- Authority
- CN
- China
- Prior art keywords
- hatching
- embryonic development
- mauremys mutica
- embryonated egg
- phase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/02—Breeding vertebrates
Abstract
A kind of method that can judge Mauremys mutica embryonic development phase without the need for dissection, belong to the differentiation of temperature decision type Sex Determination Animal Sex and sex phenotype field.In Mauremys mutica incubation season, collection Mauremys mutica embryonated egg is hatched；The situation of change of fertilization spot is observed in whole hatching process, situation of change the Taking Pictures recording that the trace of blood is observed by illumination, first week Temporal variation of hatching is quickly and embryo's too little which cannot being visually observed accurately is judged, hatching first week does not carry out embryo's anatomic observation, dissect after starting within 8th day to take 4～6 pieces of embryonated egg observation appearances daily from hatching, in stereomicroscopy Microscopic observation embryonic development situation, word description Taking Pictures recording is carried out, the embryonic development phase of Shelled Turtle Trionyx Sinensis is divided by the criteria for classifying of Mauremys mutica embryonic development phase.Embryonated egg need not be dissected, by embryonated egg visual inspection can obtain Mauremys mutica temperature sensitive period start before terminate the accurate development phase of whole process to embryonic development.
Description
Technical field
The invention belongs to the differentiation of temperature decision type Sex Determination Animal Sex and sex phenotype field, more particularly to a kind of
Can be without the need for dissecting the method for judging Mauremys mutica embryonic development phase.
Background technology
Mauremys mutica (Mauremys mutica Cantor 1842) is subordinate to Reptilia (Reptilia), Chelonia
(Testudinata), fresh water Testudinidae (Emydidae), intend Clemmys (Mauremys), due to natural propagation limited in one's ability, into
For endangered species, " CITES (CITES) " is listed in.Research shows that Mauremys mutica belongs to
In temperature Sex Determination type animal, i.e., the sex which raises up seed depends on a certain specific time period during incubating oosperm
(temperature sensitive period).High temperature hatching during this period can be produced compared with polygeny tortoise seedling, otherwise is produced compared with polyandry tortoise seedling.Yellow in recent years
Larynx intends the concern that terrapin enjoys people, one band of special Guangdong and Guangxi Provinces.
As the sex of temperature Sex Determination type animal is that (tortoise is most 15～17 in the temperature sensitive period of its embryonic development
Phase) in determined by temperature, therefore, the accurate division (particularly after 14 phases) of its embryonic development phase is adjusted for its sex
Control is extremely important.The division methods of embryonic development phase are determined typically by regular anatomic observation.Outside by embryonated egg
Observation and anatomic observation, obtain the very strong data of correlation.
Yntema is generally adopted in the world【1】Staging scale carry out (being divided into for 26 phases) division of tortoise embryonic development phase, Zhu
The new flat embryonic development (being divided into for 22 phases) for reporting Mauremys mutica.
There are reports for the domestic and international patent of invention with regard to Mauremys mutica, relates generally to Mauremys mutica incubation side
Method【2】, yellow larynx microsatellite marker and its application【3】, intend terrapin disease prevention method【4】And its raise and feed formulation method
Deng【5-7】.So far there are no to the report about judging testudinate embryonic development phase by embryonated egg outward appearance.
Bibliography：
【1】Yntema C.A series of stages in the embryonic development of
Chelydra serpentine[J].Journal of Morphology,1968,125:219-251.
【2】Zhu Xinping, Wei Cheng Qing etc..2007. Mauremys mutica incubation method.Patent application number：200510121367.X.
Application publication number：CN 1799346A, date of publication：2007.07.12.
【3】Zheng Rongquan, Zhang Yun etc..2010. a kind of Mauremys mutica microsatellite markers and application thereof.Patent application number：
201010153551.3. application publication number：101880659 A of CN, date of publication：2010.11.10.
【4】Zhaoyang.A kind of 2015. Mauremys mutica disease prevention methods.Patent application number：201410625354. application
Publication No.：104365550 A of CN, date of publication：2015.02.25.
【5】Zhaoyang.A kind of 2015. method for breeding of Mauremys mutica.Patent application number：201410625352. application is public
Cloth number：104365549 A of CN, date of publication：2015.02.25.
【6】Zhaoyang.2015. a kind of feeds of Mauremys mutica and preparation method thereof.Patent application number：
201410625268. application publication number：104397390 A of CN, date of publication：2015.03.11.
【7】Yu Zhenqing.The method that a kind of 2015. families raise Mauremys mutica.Patent application number：
201410626494.4. application publication number：104430154 A of CN, date of publication：2015.03.25.
【8】Lei Sijia, Ye Shizhou.2015. a kind of obtain a kind of Cuora trifasciata hero tortoise seedling for obtaining high hatchability
Hatching method.Patent application number：2014010150121.4, application publication number：103875602 B of CN, date of publication：
2015.09.16.
Content of the invention
It is an object of the invention to provide a kind of can be without the need for dissecting the method for judging Mauremys mutica embryonic development phase.
The present invention is comprised the following steps：
1) in Mauremys mutica incubation season, collection Mauremys mutica embryonated egg is hatched；
2) situation of change of fertilization spot is observed in whole hatching process, the situation of change of the trace of blood is observed by illumination and is clapped
According to record, first week Temporal variation of hatching is quickly and embryo's too little which cannot being visually observed accurately is judged, thus, hatching
Do not carry out embryo's anatomic observation within first week, dissect after starting within the 8th day to take 4～6 pieces of embryonated egg observation appearances daily from hatching,
In stereomicroscopy Microscopic observation embryonic development situation, word description Taking Pictures recording is carried out, by during Mauremys mutica embryonic development
The criteria for classifying of phase is divided to the embryonic development phase of Shelled Turtle Trionyx Sinensis.
In step 1) in, the hatching apparatus that the hatching is adopted include vinyon basin and incubation medium, embryonated egg
Animal pole is lain on incubation medium upwards, and the humid control of incubation medium is 5%～7%；By hatching apparatus be placed in controllable temperature,
The wet hatching of control is indoor, and the relative humidity of hatching house is maintained at 65%～88%, and incubation temperature is controlled at 26～33 DEG C, is specifically incubated
Change process can be found in Chinese patent 2014010150121.4.
In step 2) in, the criteria for classifying by Mauremys mutica embryonic development phase is to during the embryonic development of Shelled Turtle Trionyx Sinensis
Mutually carry out division and refer to document (Yntema C.A series of stages in the embryonic development
of Chelydra serpentine[J].Journal of Morphology,1968,125:219-251).
The present invention cover Mauremys mutica embryonic development sensitive periods start before the whole process terminated until embryonic development.
The present invention has following technique effect：
(1) Mauremys mutica embryonic development phase is determined with reference to international tortoise embryonic development phase standard first,
So that the result of study for being engaged in temperature Sex Determination type terrapin Sex Differentiation with regulation and control has comparativity, it is testudinate Sex Differentiation
Comparative studies lays the foundation；
(2) the accurate decision problem of Mauremys mutica embryonic development phase is solved, particularly without embryonated egg is dissected, is only led to
Cross visual inspection and determine its embryonic development phase；
(3) lay a good foundation for improving Mauremys mutica sex phenotype technology；For manufacturing enterprise, it is common production
Person successfully carries out the qualitative breeding of Mauremys mutica and has established solid foundation.
The invention reside in embryonated egg need not be dissected, Mauremys mutica temperature can be obtained by embryonated egg visual inspection sensitive
Phase starts the front accurate development phase for terminating whole process to embryonic development.
Present invention firstly provides the method for determining Mauremys mutica embryonic development time phase dividing by visual inspection, joins first
Examine international tortoise embryonic development time phase dividing standard to divide the embryonic development of Mauremys mutica, particularly give
Mauremys mutica temperature sensitive period, starts front until embryonic development terminates whole process Mauremys mutica embryonated egg mode of appearance spy
Levy.The invention can be accurately positioned Mauremys mutica embryonic development phase, be to carry out Mauremys mutica sex phenotype research the later stage
Powerful support is provided with operation.
The present invention is first according to international Yntema (bibliography：Yntema C.A series of stages
in the embryonic development of Chelydra serpentine[J].Journal of Morphology,
1968,125:Embryonic development during terminating to embryonic development before 219-251) standard started to Mauremys mutica temperature sensitive period
Phase has carried out System describe, and the major advantage for carrying out the judgement of Mauremys mutica embryonic development phase with the present invention is：
The phase of Mauremys mutica is carried out with reference to international tortoise embryonic development time phase dividing standard (26 phase altogether)
Divide so that the research work of Mauremys mutica Sex Differentiation and sex phenotype researcher has comparativity.
Due to being judged to the development phase of Mauremys mutica by mode of appearance, reduce in research process and solve
The workload judged by animal is cutd open, operating efficiency is greatly improved.And for breeding enterprise, technical staff is without the need for by solution
Cut open, dissect Microscopic observation, without the need for high-end instrument and equipment, the judgement for developing phase just can be accurately carried out only by flashlight,
Substantially increase the operability of Mauremys mutica sex phenotype and the success rate of operation.
Description of the drawings
Fig. 1 is plate I.In FIG, (1) embryonated egg surface is seen, and (2) embryo left surface is seen, and (3) embryo right flank is seen.
12：Hatching 11 days；13：Hatching 13 days；14：Hatching 15 days；15：Hatching 17 days；16：Hatching 19 days；17：Hatching 21 days.
Fig. 2 is plate II.In fig. 2, (1) embryonated egg surface is seen, and (2) embryo left surface is seen, and (3) embryo right flank is seen.
18：Hatching 23 days；19：Hatching 26 days；20：Hatching 29 days；21：Hatching 32 days；22：Hatching 36 days；22：Hatching 40 days.
Fig. 3 is plate III.In figure 3, (1) embryonated egg surface is seen, and (2) embryo left surface is seen, and (3) embryo right flank is seen.
24：Hatching 47 days；25：Hatching 54 days；26：Hatching 60 days.
Specific embodiment
Embodiment 1：Incubated for 150 pieces in Mauremys mutica spawning season (5～July) collection Mauremys mutica embryonated egg
Change, hatching method is (referring to Chinese patent：2014010150121.4).Hatching apparatus include vinyon basin and constant temperature and humidity
Case, the animal pole of embryonated egg are lain on incubation medium upwards, and embryonated egg spacing is maintained at 1.2cm or so, and hatching is used poly- second
Alkene plastics basin is placed in controllable temperature, controls wet hatching interior (climatic chamber), and the relative humidity of hatching house is maintained at 65%～
Between 88%, incubation temperature is controlled at 29 ± 0.1 DEG C.Hatching initial stage, the relative humidity control of hatching house hatch mid-term 65%
75% is adjusted in relative humidity, and hatching later stage relative humidity is controlled 85%～88%.
The situation of change of fertilization spot is observed in whole hatching process, observes embryo's trace of blood with flashlight illumination embryonated egg
Situation of change Taking Pictures recording.4～6 pieces of embryonated egg is taken daily from hatching beginning in the 8th day carries out visual inspection, subsequently dissects vertical
Body basis of microscopic observation embryonic development situation, carries out word description Taking Pictures recording, with reference to Yntema (bibliography：Yntema
C.A series of stages in the embryonic development of Chelydra serpentine[J]
.Journal of Morphology,1968,125:The criteria for classifying centering of the tortoise embryonic development phase of description 219-251)
The embryonic development phase of magnificent soft-shelled turtle is divided.The present invention cover Mauremys mutica embryo send out sensitive periods start before the 12nd phase until
26 phases that embryonic development terminates, whole hatching process continues 60 days.At 29 DEG C, embryonic development phase is listed in table 1.
Embodiment 2：150 pieces of its embryonated egg is gathered in Mauremys mutica spawning season (5～July) to be hatched, hatching side
Method is referring to Chinese patent：2014010150121.4.Hatching apparatus include vinyon basin and climatic chamber, embryonated egg
Animal pole is lain on incubation medium upwards, and embryonated egg spacing is maintained at 1.2cm or so, and hatching vinyon basin is placed in
Wet hatching interior (climatic chamber) of controllable temperature, control, the relative humidity of hatching house are maintained between 65%～88%, hatching temperature
Degree control is at 27 ± 0.1 DEG C.Hatching initial stage, the relative humidity control of hatching house are adjusted in hatching mid-term relative humidity 65%
75%, hatching later stage relative humidity is controlled 85%～88%.
The situation of change of fertilization spot is observed in whole hatching process, observes embryo's trace of blood with flashlight illumination embryonated egg
Situation of change Taking Pictures recording.4～6 pieces of embryonated egg is taken daily from hatching beginning in the 8th day carries out visual inspection, subsequently dissects vertical
Body basis of microscopic observation embryonic development situation, carries out word description Taking Pictures recording, with reference to Yntema (bibliography：Yntema
C.A series of stages in the embryonic development of Chelydra serpentine[J]
.Journal of Morphology,1968,125:The criteria for classifying centering of the tortoise embryonic development phase of description 219-251)
The embryonic development phase of magnificent soft-shelled turtle is divided.The present invention cover Mauremys mutica embryo send out sensitive periods start before o. 11th until
26 phases that embryonic development terminates, whole hatching process continue 78 days, and at 27 DEG C, embryonic development phase is listed in table 1.
Embodiment 3：150 pieces of its embryonated egg is gathered in Mauremys mutica spawning season (the 5-7 months) to be hatched, hatching method
Referring to Chinese patent：2014010150121.4.Hatching apparatus include vinyon basin and climatic chamber, embryonated egg dynamic
Thing pole is lain on incubation medium upwards, and embryonated egg spacing is maintained at 1.2cm or so, and hatching vinyon basin is placed in can
Wet hatching interior (climatic chamber) of temperature control, control, the relative humidity of hatching house are maintained between 65%～88%, incubation temperature
Control is at 27 ± 0.1 DEG C.Hatching initial stage, the relative humidity control of hatching house are adjusted in hatching mid-term relative humidity 65%
75%, hatching later stage relative humidity is controlled 85%～88%.Incubation temperature is controlled at 32 ± 0.1 DEG C.Hatching initial stage, hatching house
Relative humidity control 65%, be adjusted to 75% in hatching mid-term relative humidity, the relative humidity control of hatching later stage 85%～
88%.
The situation of change of fertilization spot is observed in whole hatching process, observes embryo's trace of blood with flashlight illumination embryonated egg
Situation of change Taking Pictures recording.4～6 pieces of embryonated egg is taken daily from hatching beginning in the 11st day carries out visual inspection, subsequently dissects and exists
Stereomicroscopy Microscopic observation embryonic development situation, carries out word description Taking Pictures recording, with reference to Yntema (bibliography：
Yntema C.A series of stages in the embryonic development of Chelydra
serpentine[J].Journal of Morphology,1968,125:The tortoise embryonic development phase of description 219-251)
The criteria for classifying is divided to the embryonic development phase of Shelled Turtle Trionyx Sinensis.
Table 1
| Phase | 29℃ | 27℃ | 32℃ |
| 12 | 11 | 14 | 10 |
| 13 | 13 | 17 | 12 |
| 14 | 15 | 20 | 14 |
| 15 | 17 | 22 | 16 |
| 16 | 19 | 25 | 18 |
| 17 | 21 | 28 | 20 |
| 18 | 23 | 30 | 22 |
| 19 | 26 | 34 | 25 |
| 20 | 29 | 38 | 28 |
| 21 | 32 | 42 | 30 |
| 22 | 36 | 47 | 34 |
| 23 | 40 | 52 | 38 |
| 24 | 47 | 61 | 45 |
| 25 | 54 | 70 | 51 |
| 26 | 60 | 78 | 57 |
The present invention cover Mauremys mutica embryo send out sensitive periods start before the 12nd phase until embryonic development terminated for 26 phases, whole
Individual hatching process continues 54 days, and at 32 DEG C, embryonic development phase is listed in table 1.
The concrete division methods of the Mauremys mutica embryonic development phase are as follows：
According to international tortoise time phase dividing standard (bibliography：Yntema C.A series of stages in
the embryonic development of Chelydra serpentine[J].Journal of Morphology,
1968,125:Division methods 219-251) are redefined to the phase development of Mauremys mutica.According to embryonated egg outward appearance,
Whole embryo development procedure was divided into for 26 phases by embryo's age in days and embryo morphology changing features, mainly with head, four limbs, carapace and abdomen
The change of first is turned to basis of stages.
Following result is growth course of the embryonated egg under 29 DEG C of incubation temperatures.
Tenth second phase (29 DEG C, the 11st day, plate I -12)
Embryonated egg outward appearance：Fertilization spot surrounds embryonated egg about 1/2, and under illumination, trace of blood ring is big and obvious, and idiosome couches in yolk
On, eyes may be seen indistinctly
Idiosome outward appearance：There is bending in body, and afterbody is slightly crimped, and head is less, and eye pigment is more apparent, and four limbs appendage bud is bright
Aobvious, heart is projected in vitro, nearly transparent, and substantially, the allantois between hind leg is covered with the trace of blood for bounce
13rd phase (29 DEG C, the 13rd day, plate I -13)
Embryonated egg outward appearance：Fertilization spot surrounds embryonated egg about 1/2, and under illumination, trace of blood ring is big and obvious, and idiosome couches in yolk
On, eyes may be seen indistinctly.
Idiosome outward appearance：There is bending in body, and afterbody is slightly crimped, and head is less, and eye pigment is more apparent, and four limbs appendage bud is bright
Aobvious, heart is projected in vitro, nearly transparent, and substantially, the allantois between hind leg is covered with the trace of blood for bounce
Tenth fourth phase (29 DEG C, the 15th day, plate I -14)
Embryonated egg outward appearance：Fertilization spot surrounds embryonated egg more than 1/2, the thinner trace of blood cluster of visible two cluster above idiosome.
Idiosome outward appearance：Head is larger, and eyes are shiny black, and a thinner white gaps heartbeat of only remaininging is visible, fingerboard shape
Into trunk side starts carapace boundary occur
15th phase (29 DEG C, the 17th day, plate I -15)
Embryonated egg outward appearance：Fertilization spot situation of change is no longer obvious, and above idiosome, trace of blood cluster is substantially and overstriking.
Idiosome outward appearance：Eyes are shiny black, project greatly, and substantially, surrounding is smoothed fingerboard, and carapace is formed, and enteron aisle is prominent through body wall
Go out external.
16th phase (29 DEG C, the 19th day, plate I -16)
Embryonated egg outward appearance：Above idiosome, trace of blood cluster becomes apparent from overstriking and increases.
Idiosome outward appearance：Eyes are completely shiny black, and white gaps disappear, and project greatly, and groove between slight finger occurs in fingerboard, under
Jaw end reaches crystalline lens level, and carapace becomes roomy and extremely slight scultellum profile occurs, and plastron blank occurs.
17th phase (29 DEG C, the 21st day, plate I -17)
Embryonated egg outward appearance：Idiosome is visible, and eyes may be seen indistinctly, and the trace of blood is obviously prolonged overstriking and surrounds idiosome.
Idiosome outward appearance：Eyes are completely shiny black, project greatly, and lower jaw end extends beyond crystalline lens level, and muzzle starts
Existing, occur obvious serration around fingerboard, there is slight scultellum profile in carapace, and plastron substantially, is hidden to a certain extent
Heartbeat situation is kept off, but heartbeat is still visible.
18th phase (29 DEG C, the 23rd day, plate II-18)
Embryonated egg outward appearance：Idiosome, it is apparent that and darker, the trace of blood is thick, under light illumination, overall more aglow in ovum,
Ovum internal upper part starts blank parts occur.
Idiosome outward appearance：Eyes increase and prominent obvious, eyelid appearance, lower jaw end goes out beyond eyes front end level, muzzle
Existing, carapace becomes roomy, so that idiosome can not be lain on one's side completely, plastron substantially but yet there are no the boundary of plastron scultellum, and heartbeat is still visible,
Fingerboard surrounding serration is deep and obvious, and scutes of carapace profile is apparent, and tail end is no longer crimped towards belly.
19th phase (29 DEG C, the 26th day, plate II-19)
Embryonated egg outward appearance：Idiosome, it is apparent that the trace of blood gradually substantially covers whole yolk, under light illumination, in ovum overall more
For aglow, ovum internal upper part blank parts account for 1/3 on the vertical plane.
Idiosome outward appearance：Palpebra inferior substantially, but does not also reach crystalline lens level, and snout is obvious, nasal septum hyperpigmentation, muzzle
Substantially in brilliant white, the neck back side and carapace have obvious pigment deposition, and substantially, carapace rib is high-visible, carapace shield for backbone
Piece is high-visible, and the forelimb the five fingers separate, and hind leg four refers to and separate that plastron physically well develops the heartbeat situation that be almost completely covered by,
Tails elongated, its end are no longer crimped towards belly, swingable.
20th phase (29 DEG C, the 29th day, plate II-20)
Embryonated egg outward appearance：Idiosome is, it is apparent that the trace of blood is thick and substantially cover whole yolk, under light illumination, overall in ovum
Aglow, ovum internal upper part blank parts account for 1/3 on the vertical plane
Idiosome outward appearance：Palpebra inferior reaches lenticular lower limb, and clearly, pigment further adds scutes of carapace boundary
Deep, three ribs are slightly visible, and carapace substantially becomes big, and plastron has slight pigment deposition, and four limbs skin of back scale is obvious, toes portion
Separately begin claw occur, two toes of the forelimb near back have obvious pigment deposition, and four limbs are swingable, head slight oscillatory but also not
See contraction
21st phase (29 DEG C, the 32nd day, plate II-21)
Embryonated egg outward appearance：Idiosome, it is apparent that the trace of blood is thick and substantially cover whole yolk, under light illumination, ovum internal upper part
Blank parts are on the vertical plane more than 1/3.
Idiosome outward appearance：Idiosome gradually turns direction, start perpendicular lie on yolk, palpebra inferior is through lenticular following
Edge, scutes of carapace boundary clearly, further deepen by pigment, and three ribs are slightly visible, and carapace substantially becomes big, and plastron has on a small quantity
Pigment deposition, substantially, the coloring of five toes is more apparent for four limbs skin of back scale, and four limbs swing relatively flexibly, head slight oscillatory but also
Have no contraction.
20th second phase (29 DEG C, the 36th day, plate II-22)
Embryonated egg outward appearance：Idiosome, it is apparent that the trace of blood is thick and substantially cover whole yolk, under light illumination, ovum internal upper part
Blank parts account for 1/2 on the vertical plane
Idiosome outward appearance：Palpebra inferior covers most of pupil, and overall pigmentation increases, and carapace starts ossified, scultellum
Become coarse, it is seen that protuberant particles, substantially, the visible pigment of first bridge, four limbs skin of back scale are more coarse for plastron pigmentation
Be in lead, claw pigmentation substantially, is wrapped in translucent pawl sheath, four limbs flexible swinging, head can free shrink,
Enteron aisle is retracted in vivo.
23rd phase (29 DEG C, the 40th day, plate III-23)
Embryonated egg outward appearance：Idiosome is big and black, and under light illumination, ovum internal upper part blank parts account for 1/2 on the vertical plane
Idiosome outward appearance：Body substantially becomes big, and upper palpebra inferior can't fully come together, and overall pigmentation becomes apparent from, and is in
Lead, clearly in black, claw pigment sinks down into toe end, is wrapped in translucent pawl sheath, four plastron pigment
Limb is more flexible to swing but can't support body, head to shrink stronger
20th fourth phase (29 DEG C, the 47th day, plate III-24)
Embryonated egg outward appearance：Idiosome is bigger and black, and the trace of blood of idiosome periphery is thick, and ovum internal upper part blank parts are on the vertical plane
More than 1/2.
Idiosome outward appearance：Body size is close with yolk, and its length basically reaches maximum, and upper palpebra inferior can close up, and four limbs are more
Can flexibly mop floor and creep, the nearly black of overall pigmentation, claw integrally in black, are still enclosed in pawl sheath.
25th phase (29 DEG C, the 54th day, plate III-25)
Embryonated egg outward appearance：Ovum internal upper part is entirely empty, and only remaining bottom idiosome is black dull, and the trace of blood around idiosome is obvious
Idiosome outward appearance：Yolk size for body 1/2, global feature is similar to the young tortoise of shell is gone out, and four limbs are strong, can support body
Body is creeped.
26th phase (29 DEG C, the 54th day, plate III-25)
Embryonated egg outward appearance：Ovum internal upper part is entirely empty, and only remaining bottom idiosome is black dull, and the trace of blood around idiosome disappears.
Idiosome outward appearance：Yolk is all absorbed, and only stays next slight gap in plastron central authorities, and some are kept well completely,
Carapace is flattened, and pawl sheath is worn away.
Claims (3)
1. a kind of can without the need for dissect judge Mauremys mutica embryonic development phase method, it is characterised in that comprise the following steps：
1) in Mauremys mutica incubation season, collection Mauremys mutica embryonated egg is hatched；
2) situation of change of fertilization spot is observed in whole hatching process, the situation of change note of taking pictures that the trace of blood is observed by illumination
Record, first week Temporal variation of hatching is quickly and embryo's too little which cannot being visually observed accurately is judged, thus, hatching first
Zhou Bingwei carries out embryo's anatomic observation, dissects, vertical after starting within the 8th day to take 4～6 pieces of embryonated egg observation appearances daily from hatching
Body basis of microscopic observation embryonic development situation, carries out word description Taking Pictures recording, by Mauremys mutica embryonic development phase
The criteria for classifying is divided to the embryonic development phase of Shelled Turtle Trionyx Sinensis.
2. a kind of as claimed in claim 1 can be without the need for dissecting the method that judge Mauremys mutica embryonic development phase, its feature exists
In in step 1) in, the hatching apparatus that the hatching is adopted are comprising vinyon basin and incubation medium, the animal pole of embryonated egg
Lain on incubation medium upwards, the humid control of incubation medium is 5%～7%；By hatching apparatus be placed in controllable temperature, control wet
Hatching is indoor, and the relative humidity of hatching house is maintained at 65%～88%, and incubation temperature is controlled at 26～33 DEG C.
3. a kind of as claimed in claim 1 can be without the need for dissecting the method that judge Mauremys mutica embryonic development phase, its feature exists
In in step 2) in, the concrete division methods of the Mauremys mutica embryonic development phase are as follows：
Division methods according to international tortoise time phase dividing standard are redefined to the phase development of Mauremys mutica,
Whole embryo development procedure was divided into for 26 phases according to embryonated egg outward appearance, embryo's age in days and embryo morphology changing features, mainly with head
The change of portion, four limbs, carapace and plastron is turned to basis of stages.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611002375.7A CN106489843A (en) | 2016-11-14 | 2016-11-14 | A kind of method that can judge Mauremys mutica embryonic development phase without the need for dissection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611002375.7A CN106489843A (en) | 2016-11-14 | 2016-11-14 | A kind of method that can judge Mauremys mutica embryonic development phase without the need for dissection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106489843A true CN106489843A (en) | 2017-03-15 |
Family
ID=58324460
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611002375.7A Pending CN106489843A (en) | 2016-11-14 | 2016-11-14 | A kind of method that can judge Mauremys mutica embryonic development phase without the need for dissection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106489843A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109164107A (en) * | 2018-10-09 | 2019-01-08 | 辽宁大学 | A kind of device and method of non-destructive testing field Parus varius egg hatching time |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5011780A (en) * | 1987-06-19 | 1991-04-30 | The Agricultural And Food Research Council | In vitro embryo culture technique |
| US7253334B2 (en) * | 2000-12-22 | 2007-08-07 | Aurox, Llc | Methods for cloning non-human mammals using reprogrammed donor chromatin or donor cells |
| CN104982358A (en) * | 2015-06-17 | 2015-10-21 | 贵州宏康生态养殖有限责任公司 | Salamander ecological cultivation method |
| CN105724310A (en) * | 2016-02-16 | 2016-07-06 | 深圳职业技术学院 | Method for judging time phase of embryonic development of Chinese softshell turtles without anatomy |
-
2016
- 2016-11-14 CN CN201611002375.7A patent/CN106489843A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5011780A (en) * | 1987-06-19 | 1991-04-30 | The Agricultural And Food Research Council | In vitro embryo culture technique |
| US7253334B2 (en) * | 2000-12-22 | 2007-08-07 | Aurox, Llc | Methods for cloning non-human mammals using reprogrammed donor chromatin or donor cells |
| CN104982358A (en) * | 2015-06-17 | 2015-10-21 | 贵州宏康生态养殖有限责任公司 | Salamander ecological cultivation method |
| CN105724310A (en) * | 2016-02-16 | 2016-07-06 | 深圳职业技术学院 | Method for judging time phase of embryonic development of Chinese softshell turtles without anatomy |
Non-Patent Citations (2)
| Title |
|---|
| 吴美仙,赵波,张文,陆洪良: "孵化温度对黄喉拟水龟胚胎发育和幼体特征的影响", 《生态学报》 * |
| 赵伟华,朱新平,魏成清,杜合军,陈永乐: "黄喉拟水龟胚胎发育的观察", 《水生生物学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109164107A (en) * | 2018-10-09 | 2019-01-08 | 辽宁大学 | A kind of device and method of non-destructive testing field Parus varius egg hatching time |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Donoughe et al. | Embryonic development of the cricket Gryllus bimaculatus | |
| del Pino et al. | Embryonic stages of Gastrotheca riobambae (Fowler) during maternal incubation and comparison of development with that of other egg‐brooding hylid frogs | |
| Gongruttananun | Influence of red light on reproductive performance, eggshell ultrastructure, and eye morphology in Thai-native hens | |
| Gupta et al. | Turkey embryo staging from cleavage through hypoblast formation | |
| Dettlaff et al. | Animal Species for Developmental Studies: Vertebrates | |
| Morioka et al. | Growth and morphological development of laboratory-reared larval and juvenile Pangasianodon hypophthalmus | |
| Vieira et al. | Description of embryonic stages in Melanosuchus niger (Spix, 1825)(Crocodylia: Alligatoridae) | |
| Rodríguez et al. | Eye development and the role of vision during Siberian sturgeon early ontogeny. | |
| Rozen Jr et al. | Investigations on the biologies and immature stages of the cleptoparasitic bee genera Radoszkowskiana and Coelioxys and their Megachile hosts (Hymenoptera: Apoidea: Megachilidae: Megachilini) | |
| CN102100193A (en) | Method for breeding pearls by combining cell small pieces of black lip shells with pinctada martensii dunker | |
| CN104756949A (en) | Artificial incubating and brooding method for Grus japonensis | |
| CN106489843A (en) | A kind of method that can judge Mauremys mutica embryonic development phase without the need for dissection | |
| Liu et al. | Broodstock conditioning and spawning | |
| Willier | Hormonal control of embryonic differentiation in birds | |
| Flajšhans et al. | A review on the potential of triploid tench for aquaculture | |
| CN105123575A (en) | Method for continuous observation of Tiretrack eel embryonic development | |
| CN105724310A (en) | Method for judging time phase of embryonic development of Chinese softshell turtles without anatomy | |
| Radael et al. | Morphophysiological characterization of the embryonic development of Melanotaenia praecox (Weber & de Beaufort, 1922) | |
| da Cruz Mattos et al. | Description of the ontogenic and larval period of discus fish (Symphysodon aequifasciatus) | |
| Rozen et al. | Biology and morphology of the immature stages of the cleptoparasitic bee Coelioxys chichimeca (Hymenoptera: Apoidea: Megachilidae) | |
| Tong et al. | Stages of embryonic development and changes in enzyme activities in embryogenesis of turbot (Scophthalmus maximus L.) | |
| CN107950481A (en) | A kind of supporting breeding method of the first five pawls green grass or young crops shin corollule spot plumage chicken of phoenix | |
| Wu et al. | Pigmentation formation and expression analysis of tyrosinase in Siniperca chuatsi | |
| CN105475183A (en) | Seawater pearl culture pearl producing and nuclear insertion method | |
| KR20130057722A (en) | Method for mass producing artificial seed of abudefduf vaigiensis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170315 |
|
| RJ01 | Rejection of invention patent application after publication |