CN106479884A - Continuous rotary drum Aspergillus niger strain solid state fermentation spreads cultivation and spore production equipment - Google Patents
Continuous rotary drum Aspergillus niger strain solid state fermentation spreads cultivation and spore production equipment Download PDFInfo
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- CN106479884A CN106479884A CN201610838867.3A CN201610838867A CN106479884A CN 106479884 A CN106479884 A CN 106479884A CN 201610838867 A CN201610838867 A CN 201610838867A CN 106479884 A CN106479884 A CN 106479884A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
Abstract
The present invention relates to a kind of continuous rotary drum Aspergillus niger strain solid state fermentation spreads cultivation and spore production equipment, the radially opposite sides of the middle part tubular tank body of its rotary drum fermentation tank are symmetrically fixedly mounted with horizontal support set, the first level support set of side is outside to be connected with driving means and is driven by, the first pipeline stretching to tank interior and connecting steam sterilizer, aseptic ventilation unit is installed in first level support set, and connecting classification inoculation apparatus, the second pipeline of water replanishing device, the second pipeline connects the liquid distribution trough in tank body；In second horizontal support set of opposite side, water inlet and outlet sleeve pipe is installed, is provided with chuck outside the tank body of rotary drum fermentation tank, this chuck passes through the telescopic joint heat-exchange device of Inlet and outlet water；On-line monitoring device includes the sensor unit being connected with control system.The present invention can obtain high-volume, high-quality strain product within a short period of time, and production efficiency is high, production process safety, reduce inoculation, sterilizing, the expense in sweat, raising economy simultaneously.
Description
Technical field
The invention belongs to bioreactor technology field, particularly a kind of continuous rotary drum Aspergillus niger strain solid state fermentation
Spread cultivation and spore production equipment.
Background technology
Solid fermentation has easy and simple to handle, and energy consumption is low, and fermentation period is short, the advantages of downstream processes are simple, at present, perhaps
Many organic acid, protein, alcohol product all to be produced using microorganism solid fermentation method, but be subject to due to its sweat
Influence factor's a lot (water content of substrate, granularity, pH value, fermentation temperature, gas phase composition and Ventilation Rate etc.), and on-line monitoring
Means are perfect not to the utmost, and related research is also relatively slower.With regard to solid bran koji industrially prepared for, spreading cultivation of strain still adopts
Traditional technique：Former optimization strain inclined plane strain Fructus Solani melongenae bottle strain triangular flask Fuqu.So spread cultivation from original seed
Strain amount needed for fermentation tank, needs to prepare the Fuqu of hundreds of triangular flask, and strain spreads cultivation the process amount of labour greatly, and one three
Angle bottle Fuqu microbiological contamination, will lead to whole fermentation tank strain all unavailable, and that is, Fuqu microbiological contamination probability is high.The present invention is a kind of Fuqu bacterium
Kind of extensive preparation facilitiess, substitute the propagation method of hundreds of triangular flask, realize the standardization of strain production it is ensured that product same
Criticize homogeneity.
A kind of portable lateral ventilation thick-layer Zymolysis Equipment of patent ZL 03159569.3 invention scale fermentation,
This invention can make multiple reactors in series.And its bottom can lean on gravity automatic-discharging tunning for inclined-plane after fermentation ends.
But large-scale production needs tandem reactor, makes floor space, and operating cost increases.
Patent CN 102358883 A devises a kind of rotating-shaft-type solid fermenting jar, can achieve that atomization is mended inside this fermentation tank
Water, uniform moisture, humid control high precision；And it is provided with chuck, shaft, stirring paddle, that is, temperature control precision is also high.But should
Device interior construction is complicated, and cost is high, and cleans difficult.
Patent CN 102839119 A has invented a kind of solid-state fermenter, the stirring system of this fermentation tank by wall scraper plate,
Bottom surface scraper plate and the frame like structure of stirrer paddle composition, energy effectively solving thickness of feed layer is uneven, and a difficult problem for inequality of being heated is realized certainly
The batch production of dynamicization.
Patent CN 102318789 A has invented a kind of solid state fermentation equipment and has prepared Monas cuspurpureus Went using this solid state fermentation equipment
Method.This equipment to supply material using axial supply pipe, and stirring vane is arranged in supply pipe, when equipment rotation
When, stirring and the effect of batch mixing can be played.And whole fermentation process solves inside fermentation tank it is therefore prevented that living contaminantses and mycete
Toxin produces.But, the growth of impact thalline big to material cutting power using mechanical agitation mode.
A kind of full visible microorganism solid-state fermentation tank of patent CN 202030749 U, this tank body can rotate, the axle of hollow
Stretch in fermentation tank, axle has aperture, ventilation in achievable tank.And tank body (inclusion lid) is transparent engineering plastics material
Matter, can observe the fermentation situation in tank at any time.
Design accordingly, with respect to Zymolysis Equipment should be set about in temperature so that material can either be promoted from controlling bed humidity
Mixing it is ensured that bed of material property is homogeneous, and optimize in the environment of, be unlikely to cause large effect to the growth of thalline again.
Also it is considered as its cost and operating characteristics simultaneously.
Content of the invention
It is an object of the invention to overcoming the deficiencies in the prior art, provide a kind of can the radiating of effectively solving fermentation tank, mass transfer,
The problems such as sterilizing is difficult, and ensure that the aseptic solid state bacterial of operating process spreads cultivation and Zymolysis Equipment.
The present invention solves its technical problem and is achieved through the following technical solutions：
A kind of continuous rotary drum Aspergillus niger strain solid state fermentation spread cultivation and spore production equipment it is characterised in that：Including
Rotary drum fermentation tank, driving means, heat-exchange device, steam sterilizer, aseptic ventilation unit, classification inoculation apparatus, water replanishing device,
Line supervising device and control system, the tank body of rotary drum fermentation tank includes upper and lower end tapered shaped can body and middle part tubular tank body, middle part
The radially opposite sides of tubular tank body are symmetrically fixedly mounted with horizontal support set, and two horizontal support sets are respectively arranged on the support of bearing of both sides；
The first level support set of side is outside to be connected with driving means and is driven by, and installs and stretch in first level support set
Tank interior simultaneously connects the first pipeline of steam sterilizer, aseptic ventilation unit, and connect classification inoculation apparatus, the of water replanishing device
Two pipelines, the second pipeline connects the liquid distribution trough in tank body；In second horizontal support set of opposite side, water inlet and outlet sleeve pipe is installed,
It is provided with chuck, this chuck passes through the telescopic joint heat-exchange device of Inlet and outlet water outside the tank body of rotary drum fermentation tank；On-line monitoring fills
Put including the Temperature Humidity Sensor being connected with control system in tank body, CO2Concentration detector and O2Concentration detector.
And, described driving means include reduction box and motor, and the output gear of reduction box is installed on described
Outside first level support set, motor drives the rotation of first level support set by reduction box, and then drives rotary drum fermentation
Tank rotates.
And, it is provided with demarcation strip in the chuck of setting outside described rotary drum fermentation tank, form the internal layer folder being connected
Set and outer layer chuck, the water inlet connection internal layer chuck of Inlet and outlet water sleeve pipe, the outlet connection outer layer chuck of Inlet and outlet water sleeve pipe, enters
Water outlet sleeve Gutron crosses outlet and the water return outlet that swivel joint connects heat-exchange device.
And, observation panel is provided with the taper tank body of rotary drum fermentation tank, enters (going out) material mouth, sample tap, tail gas discharge
Mouthful, each one of the both sides of the taper tank body in fermentation tank for the described observation panel.
And, by seed storage tank, peristaltic pump forms described classification inoculation apparatus, and seed storage tank passes through peristaltic pump and the first pipeline
Connect described liquid distribution trough.
And, by aseptic water storage tank, peristaltic pump forms described water replanishing device, and aseptic water storage tank passes through peristaltic pump and first
Pipeline connects to described liquid distribution trough.
And, described ventilation unit is by air compressor, air disinfector, air humidifier, air preheater successively
Connect the gas distributor within forming and connecting to rotary drum fermentation tank by the second pipeline.
And, described steam sterilizer includes steam generator, and steam generator is connected to institute by the second pipeline
State gas distributor.
And, described gas distributor is the diminishing serpentine pipe of screw diameter, and serpentine pipe is formed with gas distribution
Hole.
Advantages of the present invention and having the beneficial effect that：
1st, this continuous rotary drum Aspergillus niger strain solid state fermentation spreads cultivation and spore production equipment, and overall employing of fermentation tank turns
Drum-type structure, the cone barrel tank skin at two ends has certain angle (30-60 DEG C), in so whole tank body with middle cylindrical shape tank skin
The cross section in chamber is of different sizes, when tank body rotates, can promote the mixing of material, improves material and material, material and air
Heat transfer, it is ensured that the homogeneity of bed property, this can substitute the stirring paddle of traditional zymotic tank to mass transfer effect to a certain extent, with
When be easy to cleaning in tank.It is effectively reduced triangular flask, the use of the equipment such as incubator, decrease the several of microbiological contamination in incubation
Rate, to saving production cost, improves strain quality significant.
2nd, this continuous rotary drum Aspergillus niger strain solid state fermentation spreads cultivation and spore production equipment, and driving means adopt stepping
Motor is connected with the first level support set outside rotary drum fermentation tank by gear reduction unit, and motor can be believed according to pulse
Number frequency and positive and negative automatic regulation rotating speed and steering, the rotating speed that rotary drum fermentation tank rotates and direction are controlled with this.Need
Illustrate, front described gear reduction unit can be replaced by turbine and worm decelerator or conveyer belt.
3rd, this continuous rotary drum Aspergillus niger strain solid state fermentation spreads cultivation and spore production equipment, arranges outside rotary drum fermentation tank
There is folder, be connected with water tank with outlet by water inlet pipe, the import and export of chuck internal water are opened in tank body second horizontal support set
Side, and whole jacket space is become upper and lower two-layer by divides, thus avoiding the short circuit of water circulation, sufficiently utilizes heat exchange
Area, and demarcation strip welded together with jackets inner wall and tank wall, can rotate together with tank body, the import and export of jacket water (J.W.)
Pipeline adopts swivel joint to connect, and when so can guarantee that fermentation tank rotation, still can be passed through the water for heat exchange in chuck,
Thus realizing the cooling of fermentation tank or heating.It is to be noted that demarcation strip can use multilamellar, or spiral, the form such as waveform
Realize the effect of water conservancy diversion.When needing steam sterilization, setting chuck coolant-temperature gage reaches 100 DEG C, when so can reduce steam sterilization
The increase of the moisture content of material that fermentation tank 9 inner chamber is led to due to the generation of condensed water.
4th, this continuous rotary drum Aspergillus niger strain solid state fermentation spreads cultivation and spore production equipment, and classification inoculation apparatus are using wriggling
Seed liquor is squeezed in fermentation tank by pump, whole seeded process seed liquor all closing pipeline in not with external contact, it is to avoid
Cause strain pollution using other vaccination ways, should ensure that the rotating speed of peristaltic pump in seeded process in below 10r/min simultaneously,
Prevent rotating speed too high to strain generation harmful effect.
5th, this continuous rotary drum Aspergillus niger strain solid state fermentation spreads cultivation and spore production equipment, the first pipeline B and rotary drum
Fermentation tank intracavity inter-connection, the different phase in whole sweat plays different effects, during inoculation, opens its peristaltic pump,
Valve a, shutoff valve b, seed liquor can be entered in fermentation tank by the first pipeline B, and through liquid distribution trough, uniform spray
In fermentation substrate；Fermentation substrate humidity reduces during the fermentation, when needing moisturizing, opens its peristaltic pump, valve b, shutoff valve
A, sterilized water can be entered in fermentation tank by the first pipeline, completes moisturizing.
6th, this continuous rotary drum Aspergillus niger strain solid state fermentation spreads cultivation and spore production equipment, the second pipeline D and rotary drum
Fermentation tank intracavity inter-connection, the different phase in whole sweat plays different effects, after material loads fermentation tank, needs
Want steam sterilization, open steam generator, valve c, shutoff valve d, steam enters inner chamber by the second pipeline D and sterilized, thus
Ensure the no miscellaneous bacteria impact of fermentation condition；During the fermentation, by opening air compressor, valve d, shutoff valve c, compressed air
Air filter, air humidifier can be passed through, air heater is passed through institute in sweat from the second pipeline D in fermentation tank
The air needing.
7th, this continuous rotary drum Aspergillus niger strain solid state fermentation spreads cultivation and spore production equipment, and gas distributor is spiral
Shape, has the gas distribution holes of 2mm in the bottom of distributor, and this distribution of shapes device can extend into the inside of fermentation substrate, right
Material plays the effect of stirring, and filtrated air can be made to be fully contacted with material simultaneously, beneficial to the discharge of supply and the heat of oxygen.
8th, this continuous rotary drum Aspergillus niger strain solid state fermentation spreads cultivation and spore production equipment, steam sterilization, and inoculation is sent out
Ferment process is all within carrying out in fermentation tank, it is to avoid the living contaminantses in operating process, and wherein inoculation is using liquid inoculation
Mode, labor intensity is little, and operating process microbiological contamination probability is little.
9th, this continuous rotary drum Aspergillus niger strain solid state fermentation spreads cultivation and spore production equipment, central control board and rotary drum
Fermentation tank, motor, peristaltic pump, water pump, air compressor etc. connect, and can control motor in real time, and air compressor is compacted
The switch of dynamic pump, water pump and all kinds of electromagnetic valves, the rotating speed of fermentation tank in real-time control sweat, rotation direction, and fermentation
Temperature in tank, humidity, the condition such as air mass flow it is ensured that strain is under optimized environmental condition growth, thus at utmost
Utilization resource, improve production efficiency, and achieve automated production.
10th, the present invention can obtain high-volume, high-quality strain or other fermented products within a short period of time, produces effect
Rate is high, and production process safety meanwhile, reduces inoculation, the expense in the expense and sweat of sterilizing, improves it economical
Property.
Brief description
Fig. 1 is the flowage structure schematic diagram of the present invention；
Fig. 2 is the sectional view of the rotary drum fermentation tank of the present invention；
Fig. 3 is the E-E of Fig. 1 to sectional view；
Fig. 4 is the top view of the liquid distribution trough of the present invention.
Description of reference numerals
1- heater, 2- water tank, 3- outlet pipe, 4- electric control valve, 5- swivel joint, 6- water pump, 7- water inlet pipe, 8-
Two supports of bearing, 9- rotary drum fermentation tank, 10- chuck, 11- liquid distribution trough, 12- Temperature Humidity Sensor, 13- gas distributor,
14- observation panel, 15- enter (going out) material mouth, 16- clutch shaft bearing support, 17- reduction box, 18- motor, 19-CO2Concentration Testing
Instrument, 20-O2Concentration detector, 21- peristaltic pump, 22- seed storage tank, the aseptic water storage tank of 23-, 24- air preheater, 25- air
Humidification, 26- air disinfector, 27- steam generator, 28- air compressor, 29- touch screen, 30- switch board, 31- first water
Flat support set, 32- the second horizontal support set, 33- gas distribution holes.
Specific embodiment
Below by specific embodiment, the invention will be further described, and following examples are descriptive, is not limit
Qualitatively it is impossible to protection scope of the present invention is limited with this.
A kind of continuous rotary drum Aspergillus niger strain solid state fermentation spreads cultivation and spore production equipment, and it includes rotary drum fermentation tank
9th, driving means, heat-exchange device, steam sterilizer, aseptic ventilation unit, classification inoculation apparatus, water replanishing device, on-line monitoring dress
Put and control system.The tank body of rotary drum fermentation tank includes upper and lower end tapered shaped can body and middle part tubular tank body, middle part tubular tank body
Radially opposite sides be symmetrically fixedly mounted with horizontal support set, two horizontal support sets are respectively arranged on the support of bearing of both sides.The of side
One horizontal support set rotation is installed on clutch shaft bearing support 16, and the first level support set 31 of side is outside with driving means even
Connect and be driven by.Fermentation tank 9 totally adopts rotary drum structure, and the rotary drum structure at two ends has certain angle with middle cylinder
(30-60℃).The cross section of so whole tank inner chamber is of different sizes.When tank body rotates, the mixing of material can be promoted, improve
Material and material, the heat transfer of material and air, mass transfer effect is it is ensured that the homogeneity of bed property.This can replace to a certain extent
For the stirring paddle of traditional zymotic tank, simultaneously facilitate cleaning in tank.The taper tank body of rotary drum fermentation tank is provided with observation panel 14,
Enter (going out) material mouth 15, sample tap, tail gas outlet, each one of the both sides of the taper tank body in fermentation tank for the observation panel.
Driving means include reduction box 17 and motor 18, and the output gear of reduction box is installed on first level support set
Outside, motor drives the rotation of first level support set by reduction box, and then drives the rotation of rotary drum fermentation tank.Can realize
The rotation of tank body arbitrary speed and start and stop at any angle.Motor can be automatic with positive and negative according to the frequency of pulse signal
Regulation rotating speed and steering, the rotating speed that fermentation tank 9 rotation is controlled with this and direction.It should be noted that front described gear subtracts
Fast device 17 can be replaced by turbine and worm decelerator or conveyer belt.
Install in first level support set and stretch to tank interior and connect steam sterilizer, aseptic ventilation unit
First pipeline D, and connect classification inoculation apparatus, the second pipeline B of water replanishing device, the second pipeline connects the liquid distribution trough in tank body
11.
Classification inoculation apparatus are made up of seed storage tank 22, peristaltic pump 21, and seed storage tank passes through peristaltic pump and the first pipeline B connection liquid
Body distributor.Water replanishing device is made up of aseptic water storage tank 23, peristaltic pump 21, and aseptic water storage tank passes through peristaltic pump and shared second
Pipeline B connects to liquid distribution trough.Using peristaltic pump 21, seed liquor is squeezed in fermentation tank 9, whole seeded process seed liquor is all
In the pipeline of closing not with external contact, it is to avoid cause strain pollution using other vaccination ways, simultaneously in seeded process
In should ensure that the rotating speed of peristaltic pump in below 10r/min, prevent rotating speed too high to strain produce harmful effect.
Second pipeline B and aforesaid fermentation tank 9 intracavity inter-connection, the different phase in whole sweat plays different works
With.During inoculation, open wriggling 21, valve a, shutoff valve b, seed liquor can be entered in fermentation tank by pipeline B, and through liquid
Distributor 11, uniformly sprays in fermentation substrate.Fermentation substrate humidity reduces during the fermentation, when needing moisturizing, opens
Peristaltic pump 21, valve b, shutoff valve a, sterilized water can be entered in fermentation tank by pipeline B, completes moisturizing.
Steam sterilizer includes steam generator 27, and steam generator is connected to gas by the second pipeline D sharing
Distributor 13.Ventilation unit is connected successively by air compressor 28, air disinfector 26, air humidifier 25, air preheater 24
Connect the gas distributor 13 within forming and connecting to rotary drum fermentation tank by the first pipeline D.Gas distributor is screw diameter
Diminishing serpentine pipe, serpentine pipe is formed with gas distribution holes 33.This distribution of shapes device can extend into the interior of fermentation substrate
Portion, plays the effect of stirring to material, and filtrated air can be made to be fully contacted with material simultaneously, beneficial to the row of supply and the heat of oxygen
Go out.
First pipeline D and aforesaid fermentation tank 9 intracavity inter-connection, the different phase in whole sweat plays different works
With.After material loads fermentation tank 9, need steam sterilization, open steam generator 27, valve c, shutoff valve d, steam passes through first
Pipeline D enters inner chamber and is sterilized, thus ensureing the no miscellaneous bacteria impact of fermentation condition.During the fermentation, by opening air
Compressor 28, valve d, shutoff valve c, compressed air can pass through air filter 26, air humidifier 25, air heater 24 by
First pipeline D is passed through required air in sweat in fermentation tank 9.
Second horizontal support set 32 of opposite side rotates and is installed on second bearing support 8, peace in the second horizontal support set
Put into water outlet sleeve pipe, outside the tank body of rotary drum fermentation tank, be provided with chuck 10, this chuck passes through the telescopic joint heat exchange of Inlet and outlet water
Device.It is provided with demarcation strip 34 in the chuck of setting outside rotary drum fermentation tank, form the internal layer chuck being connected and outer layer chuck,
The water inlet connection internal layer chuck of Inlet and outlet water sleeve pipe, the outlet connection outer layer chuck of Inlet and outlet water sleeve pipe, water inlet and outlet sleeve Gutron mistake
Swivel joint 5 connects water inlet pipe 7 and the outlet pipe 3 of heat-exchange device.The outside setting chuck of fermentation tank 9, by water inlet pipe 7 He
Outlet pipe 3 is connected with the water tank 2 of heat-exchange device.Electric control valve 4 is provided with outlet pipe.Setting having heaters 1 in water tank.
Water pump 6 is provided with water inlet pipe.The import and export of chuck internal water are opened in the side of tank body rotating shaft, and whole jacket space is such as schemed
Demarcation strip 10 shown in 2 is divided into inside and outside layer, thus avoiding the short circuit of water circulation, sufficiently utilizes heat exchange area, and demarcation strip 10
Weld together with jackets inner wall and tank body 9 outer wall, can rotate together with tank body.The Inlet and outlet water sleeve pipe of jacket water (J.W.) is using rotation
Joint 5 connects, and when so can guarantee that fermentation tank 9 rotation, still can be passed through the water for heat exchange in chuck, thus realizing sending out
The cooling of fermentation tank 9 or heat.It is to be noted that aforementioned demarcation strip 10 can use multilamellar, or spiral, the form such as waveform is come real
The effect of existing water conservancy diversion.When needing steam sterilization, setting chuck coolant-temperature gage reaches 100 DEG C, sends out when so can reduce steam sterilization
The increase of the moisture content of material that fermentation tank 9 inner chamber is led to due to the generation of condensed water.
On-line monitoring device includes the Temperature Humidity Sensor 12 being connected with control system in tank body, CO2 concentration detector
19 with O2 concentration detector 20.Temperature Humidity Sensor holding wire is sealed in pipeline C.Control system includes switch board 30, central authorities
Panel 29.Central control board 29 and fermentation tank 9, motor 18, peristaltic pump 21, water pump 6, air compressor 28 grade connects,
Motor 28, air compressor 1, peristaltic pump 21 can be controlled in real time, the switch of water pump 6 and each electromagnetic valve is it is ensured that microorganism exists
Grow under conditions of the most suitable, realize the Automated condtrol of system.
So that citric acid Fuqu spreads cultivation as a example, its preparation process step is as follows：
(1) batch mixing：Testa Tritici is crossed after 60 mesh sieve, weighs and proceed in right amount in fermentation tank, supplemented by pipeline A certain
The moisture of amount, being then turned on motor 18 rotor fermenter 9 makes wheat bran mix homogeneously with water；
(2) steaming：The high-temperature steam being produced using steam generator 27, when steam reaches under certain pressure, Open valve
C, make high-temperature steam with certain pressure feed fermentation tank it is ensured that pressure for a period of time, opens air vent 11 discharges steam；
(3) cool down：Isolate filtrated air using air compressor 28 and air filter 26, control valve d, will be aseptic
Air is passed through in fermentation tank 9 after air preheater preheating, cooling and fermentation tank 9 and material；
(4) separate：Open motor 18, obtain fixed rotating speed by adjusting pulse signal transducer, make fermentation tank 9 even
Continuous rotation, makes the wheat bran after cooling carry out separating, prevents material agglomerate；
(5) inoculate：Seed liquor is put under sterile conditions in inoculation tank, open peristaltic pump, adjust turning of peristaltic pump
Speed, is maintained at below 10r/min, and seed liquor slowly is driven in fermentation tank, ensures that fermentation tank rotates during inoculation,
It is easy to fermentation substrate to be sufficiently mixed with seed liquor；
(6) ferment：By air disinfector 26, air humidifier 25, air heater 24, into fermentation tank 9, it is passed through one
Determine the filtrated air of humidity and temperature, and adjust chuck coolant-temperature gage and rate of water make-up, make temperature in tank be in 33-38 DEG C, material is wet
Degree more than 80%.
(7) harvest：After the attenuation degree of aforementioned Fuqu is qualified, open the product required for obtaining into (going out) material mouth 15
Product.
Embodiments of the invention and accompanying drawing, but it will be appreciated by those skilled in the art that：Without departing from the present invention and institute
In attached spirit and scope by the claims, various replacements, to change and modifications be all possible, therefore, the scope of the present invention not office
It is limited to embodiment and accompanying drawing disclosure of that.
Claims (9)
1. a kind of continuous rotary drum Aspergillus niger strain solid state fermentation spread cultivation and spore production equipment it is characterised in that：Including turn
Drum fermentation tank, driving means, heat-exchange device, steam sterilizer, aseptic ventilation unit, classification inoculation apparatus, water replanishing device, online
Supervising device and control system, the tank body of rotary drum fermentation tank includes upper and lower end tapered shaped can body and middle part tubular tank body, middle part cylinder
The radially opposite sides of shape tank body are symmetrically fixedly mounted with horizontal support set, and two horizontal support sets are respectively arranged on the support of bearing of both sides；One
The first level support set of side is outside to be connected with driving means and is driven by, and installs and stretch to tank in first level support set
Internal portion simultaneously connects the first pipeline of steam sterilizer, aseptic ventilation unit, and connect classification inoculation apparatus, the second of water replanishing device
Pipeline, the second pipeline connects the liquid distribution trough in tank body；In second horizontal support set of opposite side, water inlet and outlet sleeve pipe is installed, turns
It is provided with chuck, this chuck passes through the telescopic joint heat-exchange device of Inlet and outlet water outside the tank body of drum fermentation tank；On-line monitoring device
Including the Temperature Humidity Sensor being connected with control system in tank body, CO2Concentration detector and O2Concentration detector.
2. continuous rotary drum Aspergillus niger strain solid state fermentation according to claim 1 spreads cultivation and spore production equipment, its
It is characterised by：Described driving means include reduction box and motor, and the output gear of reduction box is installed on described first
Horizontal support set is outside, and motor drives the rotation of first level support set by reduction box, and then drives the rotation of rotary drum fermentation tank
Turn.
3. continuous rotary drum Aspergillus niger strain solid state fermentation according to claim 1 spreads cultivation and spore production equipment, its
It is characterised by：Be provided with demarcation strip in the chuck of setting outside described rotary drum fermentation tank, formed the internal layer chuck being connected and
Outer layer chuck, the water inlet connection internal layer chuck of Inlet and outlet water sleeve pipe, the outlet connection outer layer chuck of Inlet and outlet water sleeve pipe, Inlet and outlet water
Sleeve pipe connects outlet and the water return outlet of heat-exchange device by swivel joint.
4. continuous rotary drum Aspergillus niger strain solid state fermentation according to claim 1 spreads cultivation and spore production equipment, its
It is characterised by：Observation panel is provided with the taper tank body of rotary drum fermentation tank, enters (going out) material mouth, sample tap, tail gas outlet, institute
State each one of the both sides of the taper tank body in fermentation tank for the observation panel.
5. continuous rotary drum Aspergillus niger strain solid state fermentation according to claim 1 spreads cultivation and spore production equipment, its
It is characterised by：Described classification inoculation apparatus are made up of seed storage tank, peristaltic pump, and seed storage tank passes through peristaltic pump and the first pipeline connects
Described liquid distribution trough.
6. continuous rotary drum Aspergillus niger strain solid state fermentation according to claim 1 spreads cultivation and spore production equipment, its
It is characterised by：Described water replanishing device is made up of aseptic water storage tank, peristaltic pump, and aseptic water storage tank passes through peristaltic pump and the first pipeline
Connect to described liquid distribution trough.
7. continuous rotary drum Aspergillus niger strain solid state fermentation according to claim 1 spreads cultivation and spore production equipment, its
It is characterised by：Described ventilation unit is sequentially connected by air compressor, air disinfector, air humidifier, air preheater
Gas distributor within forming and being connected to rotary drum fermentation tank by the second pipeline.
8. continuous rotary drum Aspergillus niger strain solid state fermentation according to claim 1 spreads cultivation and spore production equipment, its
It is characterised by：Described steam sterilizer includes steam generator, and steam generator is connected to described gas by the second pipeline
Body distributor.
9. the continuous rotary drum Aspergillus niger strain solid state fermentation according to claim 7 or 8 spreads cultivation and spore production equipment,
It is characterized in that：Described gas distributor is the diminishing serpentine pipe of screw diameter, and serpentine pipe is formed with gas distribution holes.
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| CN201610838867.3A CN106479884A (en) | 2016-09-20 | 2016-09-20 | Continuous rotary drum Aspergillus niger strain solid state fermentation spreads cultivation and spore production equipment |
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| CN201610838867.3A CN106479884A (en) | 2016-09-20 | 2016-09-20 | Continuous rotary drum Aspergillus niger strain solid state fermentation spreads cultivation and spore production equipment |
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Cited By (1)
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| CN109022271A (en) * | 2018-10-22 | 2018-12-18 | 沈阳化工研究院设计工程有限公司 | A kind of nitrifier of multistage amplification automatically spreads cultivation device and propagation method online |
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