CN106479844A - A kind of selenium-rich red wine and its production method - Google Patents
A kind of selenium-rich red wine and its production method Download PDFInfo
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- CN106479844A CN106479844A CN201611155905.1A CN201611155905A CN106479844A CN 106479844 A CN106479844 A CN 106479844A CN 201611155905 A CN201611155905 A CN 201611155905A CN 106479844 A CN106479844 A CN 106479844A
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Abstract
The invention discloses a kind of selenium-rich red wine and its production method, this selenium-rich red wine is prepared from for raw material by the anthocyanidin of certain proportioning, selenium-rich amino acid and Chinese liquor.The production method of this selenium-rich red wine is：By anthocyanidin, selenium-rich amino acid and white spirit mixed, soak 1 30 days, then with 10 100 microns of membrane filtration, the filtrate as selenium-rich red wine of gained.This selenium-rich red wine has very strong anti-oxidation efficacy, and can increase substantially human body blood selenium level.Its production method is simple, easy to operate, low production cost.
Description
Technical field
The present invention relates to technical field of bioengineering is and in particular to a kind of selenium-rich red wine and its production method.
Background technology
Anthocyanidin is a kind of strong natural product of oxidation resistance, few yet with anthocyanidin source, extracts anthocyanidin mistake
Anthocyanidin to be suppressed in journey to aoxidize, just can obtain the highly purified anthocyanidin of high activity.Its main cause has two：One is because cyanine
Element, from fresh raw material extraction process, is easily subject to oxidasic oxidation, leads to anthocyanidin browning；Two is anthocyanidin extracting solution
It is subject to thermal oxide unavoidably in concentration process.Therefore, extract anthocyanidin different according to raw material, need to take different methods, with
The reproducibility of protection anthocyanidin.
Selenium element is a kind of distinctive Anticancer Element Selenium, has very strong anti-oxidation efficacy.It main reason is that, selenium element
Replace element sulphur during biological cell intracellular metabolite, form seleno-amino acids, such as selenomethionine, selenocysteine and selenium
For cystine, the Glutathione being consequently formed has higher reproducibility.Because the sulfydryl that element sulphur is formed is Cellular Oxidation
The important electron transit mediator of reduction reaction, after element sulphur is replaced by selenium element, the seleno sulfydryl of formation is formed than element sulphur
Sulfydryl is more active.Here it is the antioxidation of selenium element, anticancer basic theories.Therefore, produce selenium-enriched food and improve human body
Antioxidant levels, are to improve human health level, the important means of anticancer defying age.But selenium element form has to human body selenium supplement
Material impact, inorganic salt selenium element has certain toxic and side effects after being absorbed by the body；And organic selenium, such as seleno egg ammonia
Acid, selenocysteine and selenocystine are safe selenium supplement forms, do not have any toxic and side effects to human body, when seleno egg ammonia
After acid, selenocysteine and selenocystine excess, can be stored in the form of selenium protein becomes organic selenium storehouse.Therefore raw
Produce the effective way that the selenium-enriched food rich in seleno-amino acids is human body selenium supplement.
Content of the invention
Based on above-mentioned prior art, the invention provides a kind of selenium-rich red wine and its production method, this selenium-rich red wine has
Very strong anti-oxidation efficacy, and human body blood selenium level can be increased substantially.
This production method is simple, easy to operate, low production cost.
Realizing the technical scheme that above-mentioned purpose of the present invention taken is：
A kind of selenium-rich red wine, is prepared from by the raw material of following mass fraction：
Anthocyanidin 0.1-9.99999%
Selenium-rich amino acid 0.00001-0.001%
Chinese liquor 90-99.89999%
Further, the extracting method of described anthocyanidin is as follows：
1) fresh Rhizoma Steudnerae Henryanae is cleaned, section making beating after being cooked with steam, obtain Rhizoma Steudnerae Henryanae slurry；
2) Rhizoma Steudnerae Henryanae slurry is encased in percolator, adds the ethanol solution that mass fraction is more than 95% in percolator to second
More than 80%, stirring mixing simultaneously gives free rein to percolation to the final mass fraction of alcohol, collects percolate, percolate is filtered,
Obtain filtrate；Or directly add the ethanol solution that mass fraction is more than 95% to be extracted in Rhizoma Steudnerae Henryanae slurry, filter, obtain filtrate；
3) by step 2) in gained filter vacuum be concentrated into ethanol mass fraction be less than 5%, obtain concentrated solution；
4) to step 3) in gained concentrated solution in add ethyl acetate extracted, stratification, separate after organic
Layer and water layer；
5) by step 4) water layer of gained is concentrated in vacuo, and it is dried, obtain the anthocyanidin that purity is more than 80%.
Further, by step 5) in gained anthocyanidin with mass fraction be more than 95% ethanol solution extracted, mistake
Filter, filter vacuum is concentrated, and is dried, obtains the anthocyanidin that purity is more than 90%.
Further, by step 4) gained organic layer carries out concentrate drying, obtains the chlorogenic acid that purity is more than 80%.
Further, the preparation method of described seleno-amino acids is as follows：
Semen sojae atricolor or wheat seed are soaked seed with 1-50PPM sodium selenite solution, so that it is sprouted at 25-30 DEG C, with
When give red light irradiation, intensity of illumination be 300-500LUX, after sprout growth arrives 3-5 centimetre, collect rudiment steam cook
Thoroughly, pull an oar after spreading for cooling, obtain serosity, inoculation quality is grind slurries quality 0.1-10% neutral protease pressed powder in serosity,
In 30-45 DEG C of bottom fermentation 12-48 hour after dissolving, obtain mixed liquor, add pure water to stir in mixed liquor, mistake
Filter, filter vacuum is concentrated, and is dried, obtains the seleno-amino acids of coarse powder shape；
Or, Semen sojae atricolor or wheat seed are soaked seed with 1-50PPM sodium selenite solution, so that it is sprouted at 25-30 DEG C
Send out, give red light irradiation simultaneously, intensity of illumination is 300-500LUX, after sprout growth arrives 3-5 centimetre, collect rudiment steam
Cook, add the making beating of 1-5 times amount pure water after spreading for cooling, boiling is boiled 30 minutes, is filtrated to get filtrate, filter vacuum is concentrated, be dried,
Obtain the seleno-amino acids of coarse powder shape；
Or Se-enriched egg is boiled, takes albumen, add the powder of 1-3 times of volume in albumen, described powder is silicon
One of rubber powder, silica flour, Pulvis Talci and crushed crude pearlite or arbitrarily several mixing, mix homogeneously is simultaneously pulverized, and obtains mixing
Powder, then admix the neutral protease pressed powder that quality is its quality 0.1-10% in mixed-powder, after the mixing that adds water it is in
Slurry, in 30-45 DEG C of bottom fermentation 12-48 hour, obtains mixed liquor, adds pure water to stir, mistake in mixed liquor
Filter, filter vacuum is concentrated, and is dried, obtains the seleno-amino acids coarse powder of coarse powder shape.
Described Se-enriched egg is the rudiment obtaining with the aforedescribed process as the egg under the hen of forage feed；Or
Egg under the hen fed with the sodium selenite solution of 0.1-5ppM.
Further, the number of degrees of described Chinese liquor are 8-70 degree.
A kind of production method of selenium-rich red wine is it is characterised in that comprise the steps：
By anthocyanidin, selenium-rich amino acid and white spirit mixed, soak 1-30 days, then the membrane filtration with 10-100 micron, institute
The filtrate obtaining is selenium-rich red wine.
Compared with prior art, its advantage and advantage are the present invention：
1) the selenium-rich red wine of the present invention has very strong anti-oxidation efficacy, and can increase substantially human body blood selenium level.
2) production method of the selenium-rich red wine of the present invention is simple, only needs anthocyanidin, selenium-rich amino acid and white spirit mixed to soak
, operate and its easy, with low cost needed for producing.
3) the invention provides the extracting method of anthocyanidin, simple to operation, need not chromatograph and can be obtained by high-purity
Anthocyanidin, and the anthocyanidin biological activity extracting greatly improves.
4) the invention provides the preparation method of seleno-amino acids, the method is in the Semen sojae atricolor of selenium-rich solution seed soaking or Wheat Species
Adopt the red light irradiation of 600-700 nanometer in sub- germination process, amino acid content is greatly improved.
Specific embodiment
With reference to specific embodiment, the present invention is described in detail.
Embodiment 1
A kind of selenium-rich red wine, is prepared from by the raw material of following mass fraction：
Anthocyanidin 0.1%
Selenium-rich amino acid 0.0005%
Chinese liquor 99.8995%.
The production method of above-mentioned selenium-rich red wine, its step is：
1st, the extraction of anthocyanidin：
1) fresh Rhizoma Steudnerae Henryanae is cleaned it is not necessary to peeling and incision, section making beating after being cooked with steam, obtain Rhizoma Steudnerae Henryanae slurry；
2) add the ethanol solution that mass fraction is 100% to be extracted in Rhizoma Steudnerae Henryanae slurry, filter, obtain filtrate；
3) by step 2) in gained filtrate be concentrated in vacuo at 50 DEG C ethanol mass fraction be less than 5%, obtain concentration
Liquid；
4) to step 3) in gained concentrated solution in add 0.5 times of volume ethyl acetate extracted, stand 12 hours
It is layered, after separating, obtained organic layer and water layer；
5) by step 4) water layer of gained is concentrated in vacuo at 60 DEG C, and it is dried, obtain the anthocyanidin that purity is 80.72%；
The organic layer of gained carries out concentrate drying, obtains the chlorogenic acid that purity is 81.38%；
6) by step 5) in the anthocyanidin of gained extracted with the ethanol solution that mass fraction is 200%, filter, will filter
Liquid is concentrated in vacuo at 60 DEG C, is dried, obtains the anthocyanidin that purity is 92.19%.
2nd, the preparation of seleno-amino acids：
Se-enriched egg is boiled, takes albumen, add the silica white of 1 times of volume in albumen, silica white herein can be used
Silica flour, Pulvis Talci and crushed crude pearlite replace, and mix homogeneously is simultaneously pulverized, and obtains mixed-powder, then admixes quality in mixed-powder
For the neutral protease pressed powder of mixed-powder quality 5%, it is in slurry after the stirring and evenly mixing that adds water, at 45 DEG C, enzymolysis 48 is little
When, obtain mixed liquor, add pure water to stir in mixed liquor, filter, filtrate is concentrated in vacuo at 60 DEG C, do
Dry, obtain the seleno-amino acids of coarse powder shape.
Described Se-enriched egg is the rudiment obtaining in aforementioned manners as the egg under the hen of forage feed；Or use
Egg under the hen that the sodium selenite solution of 1ppM is fed.
3rd, the preparation of selenium-rich red wine：
By anthocyanidin, selenium-rich amino acid and white spirit mixed, the number of degrees of described Chinese liquor are 35 degree, immersion 10 days, then with 10
The membrane filtration of micron, the filtrate of gained is selenium-rich red wine.
Embodiment 2
A kind of selenium-rich red wine, is prepared from by the raw material of following mass fraction：
Anthocyanidin 0.5%
Selenium-rich amino acid 0.001%
Chinese liquor 99.499%.
The production method of above-mentioned selenium-rich red wine, its step is：
1st, the extraction of anthocyanidin：
1) fresh Rhizoma Steudnerae Henryanae is cleaned it is not necessary to peeling and incision, section making beating after being cooked with steam, obtain Rhizoma Steudnerae Henryanae slurry；
2) Rhizoma Steudnerae Henryanae slurry is encased in percolator, adds the ethanol solution of mass fraction 95% in percolator to ethanol
Whole mass fraction is 80%, and stirring mixing simultaneously gives free rein to percolation, collects percolate, percolate is filtered, obtains filtrate；
3) by step 2) in gained filtrate be concentrated in vacuo at 50 DEG C ethanol mass fraction be less than 5%, obtain concentration
Liquid；
4) to step 3) in gained concentrated solution in add 1 times of volume ethyl acetate extracted, standing enter within 10 hours
Row layering, obtains organic layer and water layer after separating；
5) by step 4) water layer of gained is concentrated in vacuo at 60 DEG C, and it is dried, obtain the anthocyanidin that purity is 84.54%；
The organic layer of gained carries out concentrate drying, obtains the chlorogenic acid that purity is 85.67%.
6) by step 5) in the anthocyanidin of gained extracted with the ethanol solution that mass fraction is 100%, filter, will filter
Liquid is concentrated in vacuo at 60 DEG C, is dried, obtains the anthocyanidin that purity is 96.25%.
2nd, the preparation of seleno-amino acids：
Semen sojae atricolor or wheat seed are soaked seed with 50PPM sodium selenite solution, so that it is sprouted at 28 DEG C, give simultaneously
Red light irradiation, intensity of illumination is 400LUX, and after sprout growth arrives 3-5 centimetre, collection rudiment is well-done with steamed, beats after spreading for cooling
Slurry, obtains serosity, and in serosity, inoculation quality is the neutral protease pressed powder of grind slurries quality 1%, in 40 DEG C of bottom fermentations 12
Hour, obtain mixed liquor, add pure water to stir in mixed liquor, filter, filtrate is concentrated in vacuo at 60 DEG C, do
Dry, obtain the seleno-amino acids of coarse powder shape；
3rd, the preparation of selenium-rich red wine：
By anthocyanidin, selenium-rich amino acid and white spirit mixed, the number of degrees of described Chinese liquor are 18 degree, immersion 20 days, then with 100
The membrane filtration of micron, the filtrate of gained is selenium-rich red wine.
, compare the anthocyanidin of method of the present invention extraction and traditional water extraction carries taking the anthocyanidin that the present embodiment extracts as a example
The indices of the anthocyanidin taking, are estimated using colour value relative method and serum antioxidation method (DPPH method), concrete outcome is such as
Shown in table 1：
Table 1
As shown in Table 1, the anthocyanidin purity that the inventive method is extracted is high, colour value is high, biological activity is high.
Effect of the selenium-rich red wine of the present invention is described taking the selenium-rich red wine that the present embodiment produces as a example, with Chinese liquor for comparison,
With the selenium-rich red wine and Chinese liquor of the present invention, gavage is carried out respectively to mice, then measures the free radical scavenging activity in mice serum respectively
And Se content, concrete outcome is as shown in table 2 below：
Table 2
As shown in Table 2, the selenium-rich red wine that the present invention produces has very strong anti-oxidation efficacy, increases substantially human body blood selenium
Level.
Claims (8)
1. a kind of selenium-rich red wine is it is characterised in that be prepared from by the raw material of following mass fraction：
Anthocyanidin 0.1-9.99999%
Selenium-rich amino acid 0.00001-0.001%
Chinese liquor 90-99.89999%.
2. selenium-rich red wine according to claim 1 is it is characterised in that the extracting method of described anthocyanidin is as follows：
1) fresh Rhizoma Steudnerae Henryanae is cleaned, section making beating after cooking, obtain Rhizoma Steudnerae Henryanae slurry；
2) Rhizoma Steudnerae Henryanae slurry is encased in percolator, adds the ethanol solution that mass fraction is more than 95% in percolator to ethanol
More than 80%, stirring mixing simultaneously gives free rein to percolation to whole mass fraction, collects percolate, percolate is filtered, obtains filter
Liquid；Or directly add the ethanol solution that mass fraction is more than 95% to be extracted in Rhizoma Steudnerae Henryanae slurry, filter, obtain filtrate；
3) by step 2) in gained filter vacuum be concentrated into ethanol mass fraction be less than 5%, obtain concentrated solution；
4) to step 3) in gained concentrated solution in add ethyl acetate extracted, stratification, separate after organic layer and
Water layer；
5) by step 4) water layer of gained is concentrated in vacuo, and it is dried, obtain the anthocyanidin that purity is more than 80%.
3. selenium-rich red wine according to claim 2 it is characterised in that：By step 5) in gained anthocyanidin mass fraction
Ethanol solution more than 95% is extracted, and filters, filter vacuum is concentrated, and is dried, obtains the cyanine that purity is more than 90%
Element.
4. selenium-rich red wine according to claim 3 it is characterised in that：By step 4) gained organic layer carries out concentrate drying,
Obtain the chlorogenic acid that purity is more than 80%.
5. selenium-rich red wine according to claim 1 is it is characterised in that the preparation method of described seleno-amino acids is as follows：
Semen sojae atricolor or wheat seed are soaked seed with 1-50PPM sodium selenite solution, so that it is sprouted at 25-30 DEG C, give simultaneously
Give red light irradiation, intensity of illumination is 300-500LUX, after sprout growth arrives 3-5 centimetre, collection rudiment is well-done with steamed, stand
Pull an oar after cool, obtain serosity, inoculation quality is grind slurries quality 0.1-10% neutral protease pressed powder in serosity, after dissolving
In 30-45 DEG C of bottom fermentation 12-48 hour, obtain mixed liquor, add pure water to stir in mixed liquor, filter, will filter
Liquid is concentrated in vacuo, is dried, obtains the seleno-amino acids of coarse powder shape；
Or, Semen sojae atricolor or wheat seed are soaked seed with 1-50PPM sodium selenite solution, so that it is sprouted at 25-30 DEG C, with
When give red light irradiation, intensity of illumination be 300-500LUX, after sprout growth arrives 3-5 centimetre, collect rudiment steam cook
Thoroughly, add the making beating of 1-5 times amount pure water after spreading for cooling, boiling is boiled 30 minutes, is filtrated to get filtrate, filter vacuum is concentrated, be dried, obtain
The seleno-amino acids of coarse powder shape；
Or Se-enriched egg is boiled, takes albumen, add the powder of 1-3 times of volume in albumen, described powder is silica white,
One of silica flour, Pulvis Talci and crushed crude pearlite or arbitrarily several mixing, mix homogeneously is simultaneously pulverized, and obtains mixed-powder,
Admix the neutral protease pressed powder that quality is its quality 0.1-10% again in mixed-powder, be in slurry after the stirring and evenly mixing that adds water
Liquid, in 30-45 DEG C of bottom fermentation 12-48 hour, obtains mixed liquor, adds pure water to stir, filter in mixed liquor,
Filter vacuum is concentrated, is dried, obtains the seleno-amino acids coarse powder of coarse powder shape.
6. selenium-rich red wine according to claim 5 it is characterised in that：Described Se-enriched egg is with described in claim 5
The rudiment that obtains of method as the egg under the hen of forage feed；Or fed with the sodium selenite solution of 0.1-5ppM
Egg under hen.
7. selenium-rich red wine according to claim 1 it is characterised in that：The number of degrees of described Chinese liquor are 8-70 degree.
8. a kind of production method of the selenium-rich red wine described in claim 1 is it is characterised in that comprise the steps：
By anthocyanidin, selenium-rich amino acid and white spirit mixed, soak 1-30 days, then the membrane filtration with 10-100 micron, gained
Filtrate is selenium-rich red wine.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113403162A (en) * | 2021-08-10 | 2021-09-17 | 肖水芽 | Preparation method of special-flavor Daqu |
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| CN105985872A (en) * | 2015-03-02 | 2016-10-05 | 谢林君 | Method for brewing selenium-rich agilawood wine |
| CN106047600A (en) * | 2016-08-15 | 2016-10-26 | 滁州学院 | Purple sweet potato steeping wine and production method thereof |
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| CN102093939A (en) * | 2010-11-29 | 2011-06-15 | 高思 | Nutrition-enhanced health-care wine prepared from plant containing natural biological antioxidant |
| CN105985872A (en) * | 2015-03-02 | 2016-10-05 | 谢林君 | Method for brewing selenium-rich agilawood wine |
| CN104818183A (en) * | 2015-04-24 | 2015-08-05 | 陆开云 | Method for brewing selenium-rich tea wine with selenium-rich tea |
| CN106047600A (en) * | 2016-08-15 | 2016-10-26 | 滁州学院 | Purple sweet potato steeping wine and production method thereof |
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| CN113403162A (en) * | 2021-08-10 | 2021-09-17 | 肖水芽 | Preparation method of special-flavor Daqu |
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Application publication date: 20170308 |