CN106467900A - For processing microorganism species mixture and preparation method and the application of dyeing waste water - Google Patents
For processing microorganism species mixture and preparation method and the application of dyeing waste water Download PDFInfo
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- CN106467900A CN106467900A CN201510515547.XA CN201510515547A CN106467900A CN 106467900 A CN106467900 A CN 106467900A CN 201510515547 A CN201510515547 A CN 201510515547A CN 106467900 A CN106467900 A CN 106467900A
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Abstract
The invention provides a kind of microorganism species mixture for processing dyeing waste water and preparation method and application, described microorganism species mixture is：Possess the nonirrigated farmland small single-cell bacteria of degraded anthraquinone dyes ability, possess the bacillus cereuss of azo dyes degradation capability, possess the achromobacter of cellulose degradation ability, each bacterium is dense to be O.D600≈ 2.0, the volume ratio of described nonirrigated farmland small single-cell bacteria, bacillus cereuss and achromobacter is 1-2:1-2:1-2；Hybrid mode is prepared；The drawbacks of this mixture avoids traditional degradation bacteria method and can only remove single substrate and easily be poisoned by other materials, has the significant advantage that adding less, action time is short, removal efficiency is high.
Description
Technical field
The present invention relates to environmental technology field, especially a kind of microorganism species for processing dyeing waste water
Mixture and preparation method and application.
Background technology
Dyeing is typical highly water intensive industry, every 1 ton of textile water consumption 100-200 ton of dyeing and printing process,
Wherein 80-90% becomes waste water.Dyeing waste water source and pollutant component are sufficiently complex, have change of water quality
Greatly, the features such as content of organics height, colourity height (predominantly colored dyes), directly discharge is good for the mankind
Health and living environment bring high risks, cause the waste of water resource simultaneously.
At present, remove dyeing waste water COD and the method for colourity is mainly coagulation air-float and membrance separation.Coagulation
It is directed to add coagulant in waste water, eliminate or reduce the intergranular mutually exclusive power of water-borne glue body, make water
Middle colloidal solid is easy to mutually collide and agglomeration overlap to form larger particles or floccule body, and then from water
Separate, the method to purify waste water.Air supporting refers to form the micro-bubble of high degree of dispersion in water,
In adhesion waste water, the solid of hydrophobic group or liquid particles, form water-gas-granule three-phase mixed system, granule
After adhesion bubble, form apparent density and be less than the flco of water and float to the water surface, form scum layer and be stripped off,
Thus realizing the process of solid-liquid or Liquid liquid Separation.Membrance separation refers to use has Selective Separation function
Membrane material realizes the separation of the different component of feed liquid, purification, the process concentrating, generally micron order, bag
Include micro-filtration membrane (MF), ultrafilter membrane (UF), NF membrane (NF) and reverse osmosis membrane (RO) etc..
As physico-chemical process, there is building and operating cost height, equipment dimension in coagulation air-float and membrance separation
Shield and change related frequency, sludge output is big, the shortcomings of easily cause secondary pollution.Traditional biological method is again no
The Various Complex Organic substance that method is degraded in dyeing waste water.Therefore, new with efficient degrading bacterial strain as core
Bioanalysises become research and the application focus in this field.A kind of producing carefully is disclosed in CN201410036635
The pseudomonas putida of bacterium laccase, using the dyestuff in its oxidability degraded dyeing waste water.Hong Yiguo etc.
Separate from certain dye house effluents processing system activated sludge and obtain strains A zoR-2, there is reduction azo
The ability of dyestuff.Li Yanqin etc. isolate and purify separate from dyeing waste water long-term pollution soil colourless
Bacillus (Achromobacter sp.) bacterial strain, can be using the dyestuff of 4 kinds of different types of structure as substrate
Degraded.
However, due to dyeing waste water complicated component, a kind of bacterial strain such as material of can only degrading, clearance is then no
Method ensures, bacterial strain is also easy to be poisoned by other materials.Therefore only using the case of single kind degradation bacteria
Example is often unable to reach good permanence operation, and the scheme of mixed bacterial can efficiently solve this
One problem.The mixed bacterial of the microorganism composition of difference in functionality both can have been degraded many kinds of substance simultaneously, may be used again
Poisoned with the material avoiding each component of flora can not be degraded by it.As can be seen here, reasonably to different floras
Carry out particular combination so as to interact, promote the overall good metabolism of flora, improve the whole removing of system
Except efficiency, positive contribution will be made for Dyeing and Printing Wastewater Biological Treatment technology.
Content of the invention
The technical problem to be solved is to provide a kind of microbial bacteria for processing dyeing waste water
Group's mixture.
Another technical problem to be solved by this invention is to provide above-mentioned micro- life for processing dyeing waste water
The preparation method of thing flora mixture.
Another technical problem to be solved by this invention is to provide above-mentioned micro- life for processing dyeing waste water
The application of thing flora mixture.
For solving above-mentioned technical problem, the technical scheme is that：
A kind of microorganism species mixture for processing dyeing waste water, component is as follows：
Possess the nonirrigated farmland small single-cell bacteria (Micromonospora chersina) of degraded anthraquinone dyes ability,
Bacterium dense for O.D600≈2.0；
Possess the bacillus cereuss (Bacillus sp.) of azo dyes degradation capability, bacterium dense for O.D600≈2.0；
Possess the achromobacter (Achromobacter sp.) of cellulose degradation ability, bacterium dense for O.D600≈
2.0；
Wherein, the volume ratio of described nonirrigated farmland small single-cell bacteria, bacillus cereuss and achromobacter is 1-2:1-2:1-2.
Preferably, the above-mentioned microorganism species mixture for processing dyeing waste water, the little unit cell in described nonirrigated farmland
Bacterium is nonirrigated farmland small single-cell bacteria ATCC53710, depositary institution：American Type Culture collection warehousing (American
Type culture collection, ATCC), network address：http://www.atcc.org/, preservation is compiled
Number：ATCC No.53710.
Preferably, the above-mentioned microorganism species mixture for processing dyeing waste water, described bacillus cereuss are
Bacillus cereuss CICC23870, depositary institution：Chinese industrial Microbiological Culture Collection administrative center (China
Center of Industrial Culture Collection, CICC), address：Chaoyang District, Beijing City
No. 6 building of winebibber's bridge Road 24 institute, preservation date：On 02 11st, 2015, deposit number：CICC
No.23870.
Preferably, the above-mentioned microorganism species mixture for processing dyeing waste water, described achromobacter is
Achromobacter CICC23807, depositary institution：Chinese industrial Microbiological Culture Collection administrative center (China
Center of Industrial Culture Collection, CICC), address：Chaoyang District, Beijing City
No. 6 building of winebibber's bridge Road 24 institute, preservation date：On October 29th, 2014, deposit number：CICC
No.23807.
Preferably, the above-mentioned microorganism species mixture for processing dyeing waste water, the little unit cell in described nonirrigated farmland
The volume ratio of bacterium, bacillus cereuss and achromobacter is 1:1:1.
The preparation method of the above-mentioned microorganism species mixture for processing dyeing waste water, concrete preparation process
As follows：
(1) nonirrigated farmland small single-cell bacteria, bacillus cereuss, achromobacter are seeded in LB culture medium respectively,
30 DEG C of shaken cultivation to bacterium dense for O.D600≈2.0；
(2) in prescription ratio by the nonirrigated farmland small single-cell bacteria after cultivating, bacillus cereuss, achromobacter mixing all
Even, obtain final product.
Preferably, the preparation method of the above-mentioned microorganism species mixture for processing dyeing waste water, described
The formula of LB culture medium in step (1)：Peptone 10.0g, yeast powder in every 1000mL distilled water
5.0g, NaCl 5.0g, pH 7.0-7.2, after 121 DEG C sterilize 20 minutes, obtains final product.
The above-mentioned microorganism species mixture for processing dyeing waste water COD and color in removing dyeing waste water
The application of degree.
Preferably, the application of mentioned microorganism flora mixture, concrete grammar is：Described microorganism species
Mixture is inoculated in dyeing waste water with the inoculum concentration of percent by volume 0.5-1%, maintains dissolved oxygen 3.0
More than mg/L, cultivates 12-24 hour.
After above-mentioned medium centrifugal takes supernatant, according to《Dichromate titration (GB11914-89)》Measure COD,
According to《The mensure (GB11903-89) of water quality colourity》Measure colourity.
The invention has the beneficial effects as follows：
The above-mentioned microorganism species mixture for processing dyeing waste water, can be simultaneous for anthracene in dyeing waste water
The major pollutants such as quinone dyes, azo dyes and cellulose are degraded, it is to avoid traditional degradation bacteria
The drawbacks of method can only remove single substrate and easily be poisoned by other materials, has adding less, effect
The significant advantage that time is short, removal efficiency is high, water outlet meets《Textile dyeing and finishing industrial water pollution thing discharge mark
Accurate (GB 4287-2012)》Standard requires；In addition, the secondary that the respective substrate of multiple-microorganism degraded produces
Metabolite can be as the substrate of flora other component, thus promoting the overall good metabolism of flora；Mixing
Flora growth speed is fast, and metabolic capacity is vigorous, resists the ability of various impact loads stronger, in processing system
In more stable, it is possible to use immobilization technology formed biomembrane, not easily run off and become feeble and die；Than physics
Chemical method and traditional bioanalysises all have advantage, before treatment of dyeing wastewater field has a wide range of applications
Scape.Its preparation method is simple, easy to use, the needs of suitable scale industrial production.
Specific embodiment
With reference to specific embodiment, technical scheme of the present invention is further described.
Embodiment 1
A kind of preparation of the microorganism species mixture for processing dyeing waste water and acquisition
As follows using component：
Nonirrigated farmland small single-cell bacteria：Nonirrigated farmland small single-cell bacteria ATCC53710, depositary institution：American Type Culture collection
Warehousing (American type culture collection, ATCC), network address：
http://www.atcc.org/, deposit number：ATCC No.53710.
Bacillus cereuss：Bacillus cereuss CICC23870, depositary institution：Chinese industrial Microbiological Culture Collection pipe
Reason center (China Center of Industrial Culture Collection, CICC), address：
No. 6 building of Jiuxianqiao, Chaoyang District, Beijing City Road 24 institute, preservation date：On 02 11st, 2015, protect
Hide numbering：CICC No.23870.
Achromobacter：Achromobacter CICC23807, depositary institution：Chinese industrial Microbiological Culture Collection pipe
Reason center (China Center of Industrial Culture Collection, CICC), address：
No. 6 building of Jiuxianqiao, Chaoyang District, Beijing City Road 24 institute, preservation date：On October 29th, 2014, protect
Hide numbering：CICC No.23807.
Concrete preparation process is as follows：
(1) above-mentioned nonirrigated farmland small single-cell bacteria, bacillus cereuss, achromobacter are seeded to LB culture medium respectively
In, 30 DEG C of shaken cultivation to bacterium dense for O.D600≈2.0；
(2) in prescription ratio in table 1 by cultivate after nonirrigated farmland small single-cell bacteria, bacillus cereuss, achromobacter
Mix homogeneously, obtains final product.
Above-mentioned LB culture medium prescription：Distilled water 1000mL, peptone 10.0g, yeast powder 5.0g,
NaCl 5.0g, pH 7.0-7.2.121 DEG C sterilizing 20 minutes after standby.
Table 1
| Nonirrigated farmland small single-cell bacteria：Bacillus cereuss：Achromobacter (volume ratio) | |
| First group | 1:1:1 |
| Second group | 1:2:1 |
| 3rd group | 1:1:2 |
| 4th group | 2:1:1 |
Embodiment 2
Mixed bacterial each component Ratio Experiments
Deal with objects as dyeing waste water, the method for operation is shake flask culture.
Specific implementation step is as follows：The microorganism species that gained in embodiment 1 is used for processing dyeing waste water mix
The inoculum concentration that (first group to the 4th group) of compound presses percent by volume 0.5% puts in dyeing waste water, maintains
Dissolved oxygen, in more than 3.0mg/L, is cultivated 12 hours；Centrifuging and taking supernatant, according to《Dichromate titration
(GB11914-89)》Measure COD, according to《The mensure (GB11903-89) of water quality colourity》Measure colourity.
Result see table 2.
Table 2
Volume ratio 1 in can be seen that first group by above-described embodiment 2:1:Under 1 ratio, effect is optimum, water outlet
Meet《Textile dyeing and finishing industrial water pollution thing discharge standard》.
Embodiment 3
The removal COD of mixed bacterial and decolorization experiment
Deal with objects as dyeing waste water, the method for operation is aeration culture in 3L SBR system.
Specific implementation step is as follows：Set nonirrigated farmland small single-cell bacteria (Micromonospora chersina)
ATCC53710 is bacterium A, bacillus cereuss (Bacillus sp.) CICC23870 is bacterium B, achromobacter
(Achromobacter sp.) CICC23807 is bacterium C, obtains respectively by method described in embodiment 1 and contains
Bacterium A, bacterium B, single bacteria agent of bacterium C and A+B containing bacterium, bacterium A+C, bacterium B+C, the mixed vaccine of bacterium A+B+C
Group, additionally uses the bacillus cereuss (Bacillus sp.) without azo dyes degradation capability
CICC10275 prepares bacterium A+D+C as bacterium D, same method.Wherein, each component equalization body in mixture
Long-pending consumption is mixed.It is inoculated in dyeing waste water with the inoculum concentration of percent by volume 0.5%, maintain dissolved oxygen
In more than 3.0mg/L, cultivate 12 hours；Centrifuging and taking supernatant, according to《Dichromate titration (GB11914-89)》
Measure COD, according to《The mensure (GB11903-89) of water quality colourity》Measure colourity.Result see table 3.
Described bacillus cereuss (Bacillus sp.) CICC10275, depositary institution：Chinese industrial microorganism
Culture presevation administrative center (China Center of Industrial Culture Collection,
CICC), address：No. 6 building of Jiuxianqiao, Chaoyang District, Beijing City Road 24 institute, preservation date：2004
01 month 07 day, deposit number：CICC No.10275.
Table 3
Embodiment 4
The removal COD of mixed bacterial and decolorization experiment
Deal with objects as dyeing waste water, the method for operation is aeration culture in 3L SBR system.
Specific implementation step is as follows：Set nonirrigated farmland small single-cell bacteria (Micromonospora chersina)
ATCC53710 is bacterium A, bacillus cereuss (Bacillus sp.) CICC23870 is bacterium B, achromobacter
(Achromobacter sp.) CICC23807 is bacterium C, obtains respectively by method described in embodiment 1 and contains
Bacterium A, bacterium B, single bacteria agent of bacterium C and A+B containing bacterium, bacterium A+C, bacterium B+C, the mixed vaccine of bacterium A+B+C
Group, additionally uses the bacillus cereuss (Bacillus sp.) without azo dyes degradation capability
CICC10275 prepares bacterium A+D+C as bacterium D, same method.Wherein, each component equalization body in mixture
Long-pending consumption is mixed.It is inoculated in dyeing waste water with the inoculum concentration of percent by volume 0.5%, maintain dissolved oxygen
In more than 3.0mg/L, cultivate 24 hours；Centrifuging and taking supernatant, according to《Dichromate titration (GB11914-89)》
Measure COD, according to《The mensure (GB11903-89) of water quality colourity》Measure colourity.Result see table 4.
Table 4
Embodiment 5
The removal COD of mixed bacterial and decolorization experiment
Deal with objects as dyeing waste water, the method for operation is aeration culture in 3L SBR system.
Specific implementation step is as follows：Set nonirrigated farmland small single-cell bacteria (Micromonospora chersina)
ATCC53710 is bacterium A, bacillus cereuss (Bacillus sp.) CICC23870 is bacterium B, achromobacter
(Achromobacter sp.) CICC23807 is bacterium C, obtains respectively by method described in embodiment 1 and contains
Bacterium A, bacterium B, single bacteria agent of bacterium C and A+B containing bacterium, bacterium A+C, bacterium B+C, the mixed vaccine of bacterium A+B+C
Group, additionally uses the bacillus cereuss (Bacillus sp.) without azo dyes degradation capability
CICC10275 prepares bacterium A+D+C as bacterium D, same method.Wherein, each component equalization body in mixture
Long-pending consumption is mixed.It is inoculated in dyeing waste water with the inoculum concentration of percent by volume 1%, maintain dissolved oxygen to exist
More than 3.0mg/L, cultivates 24 hours；Centrifuging and taking supernatant, according to《Dichromate titration (GB11914-89)》
Measure COD, according to《The mensure (GB11903-89) of water quality colourity》Measure colourity.Result see table 5.
Table 5
Folding of three kinds of independent effects of composition is better than by the effect that above-described embodiment 3-5 can be seen that mixed bacterial
Value added, also superior to the effect of combination of two, water outlet meets《Textile dyeing and finishing industrial water pollution thing discharge standard》.
In addition, be also unable to reach same using the composition belonging to together in the bacterial strain replacement mixed bacterial not possessing degradation function
The removal effect of sample.The mixed bacterial of the microorganism composition of difference in functionality both can have been degraded many kinds of substance simultaneously,
The material that each component of flora can not be degraded by it can be avoided to poison again.The multiple-microorganism respective substrate of degraded
The secondary metabolite producing can promote the overall good metabolism of flora as the substrate of flora other component,
Thus the whole removing improving system removes efficiency.
Mixed bacterial of the present invention can be degraded gas chromatography in dyeing waste water, efficiently removes printing and dyeing useless
Colourity in water and COD, have cost and the double dominant run compared with chemical method simultaneously, can be pre-
This mix bacterium agent of phase will make positive contribution for dirty water living creature processing technique.
Above-mentioned with reference to embodiment to a kind of this microorganism species mixture for processing dyeing waste water and preparation
The detailed description that method is carried out with application, is illustrative rather than determinate, can be according to limited model
Enclose and include several embodiments, therefore changing and modifications under without departing from present general inventive concept, should
Belong within protection scope of the present invention.
Claims (9)
1. a kind of microorganism species mixture for processing dyeing waste water it is characterised in that：Component is as follows：
Possess degraded anthraquinone dyes ability nonirrigated farmland small single-cell bacteria, bacterium dense for O.D600≈2.0；
Possess the bacillus cereuss of azo dyes degradation capability, bacterium dense for O.D600≈2.0；
Possess the achromobacter of cellulose degradation ability, bacterium dense for O.D600≈2.0；
Wherein, the volume ratio of described nonirrigated farmland small single-cell bacteria, bacillus cereuss and achromobacter is 1-2:1-2:1-2.
2. the microorganism species mixture for processing dyeing waste water according to claim 1, it is special
Levy and be：Described nonirrigated farmland small single-cell bacteria is nonirrigated farmland small single-cell bacteria ATCC53710.
3. the microorganism species mixture for processing dyeing waste water according to claim 1, it is special
Levy and be：Described bacillus cereuss are bacillus cereuss CICC23870.
4. the microorganism species mixture for processing dyeing waste water according to claim 1, it is special
Levy and be：Described achromobacter is achromobacter CICC23807.
5. the microorganism species mixture for processing dyeing waste water according to claim 1, it is special
Levy and be：The volume ratio of described nonirrigated farmland small single-cell bacteria, bacillus cereuss and achromobacter is 1:1:1.
6. the system of the microorganism species mixture for processing dyeing waste water described in one of claim 1-5
Preparation Method it is characterised in that：Concrete preparation process is as follows：
(1) nonirrigated farmland small single-cell bacteria, bacillus cereuss, achromobacter are seeded in LB culture medium respectively,
30 DEG C of shaken cultivation to bacterium dense for O.D600≈2.0；
(2) in prescription ratio by the nonirrigated farmland small single-cell bacteria after cultivating, bacillus cereuss, achromobacter mixing all
Even, obtain final product.
7. the preparation of the microorganism species mixture for processing dyeing waste water according to claim 6
Method it is characterised in that：The formula of LB culture medium in described step (1)：Every 1000mL distilled water
Middle peptone 10.0g, yeast powder 5.0g, NaCl 5.0g, pH 7.0-7.2,121 DEG C of sterilizings 20
After minute, obtain final product.
8. the microorganism species mixture for processing dyeing waste water described in one of claim 1-5 is going
Application except COD and colourity in dyeing waste water.
9. microorganism species mixture according to claim 8 application it is characterised in that：Specifically
Method is：It is useless that described microorganism species mixture is inoculated in printing and dyeing with the inoculum concentration of percent by volume 0.5-1%
In water, maintain dissolved oxygen in more than 3.0mg/L, cultivate 12-24 hour.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108004166A (en) * | 2017-12-14 | 2018-05-08 | 中国科学院天津工业生物技术研究所 | A kind of microorganism species of efficient degradation PBAT plastic films |
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| CN102268394A (en) * | 2011-07-14 | 2011-12-07 | 北京赛富威环境工程技术有限公司 | Method of amplification culture of microorganism for waste water treatment and method of microorganism waste water treatment |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102268394A (en) * | 2011-07-14 | 2011-12-07 | 北京赛富威环境工程技术有限公司 | Method of amplification culture of microorganism for waste water treatment and method of microorganism waste water treatment |
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| LEI YU等: "Decolorization characteristics of a newly isolated salt-tolerant Bacillus sp. strain and its application for azo dye-containing wastewater in immobilized form", 《APPL MICROBIOL BIOTECHNOL》 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108004166A (en) * | 2017-12-14 | 2018-05-08 | 中国科学院天津工业生物技术研究所 | A kind of microorganism species of efficient degradation PBAT plastic films |
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