CN106434756A - Production method and application of fermented fructus crataegi flavor - Google Patents
Production method and application of fermented fructus crataegi flavor Download PDFInfo
- Publication number
- CN106434756A CN106434756A CN201610586318.1A CN201610586318A CN106434756A CN 106434756 A CN106434756 A CN 106434756A CN 201610586318 A CN201610586318 A CN 201610586318A CN 106434756 A CN106434756 A CN 106434756A
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- CN
- China
- Prior art keywords
- spices
- fermentation
- haw thorn
- mass ratio
- fructus crataegi
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/022—Refining
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/10—Chemical features of tobacco products or tobacco substitutes
- A24B15/12—Chemical features of tobacco products or tobacco substitutes of reconstituted tobacco
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/28—Treatment of tobacco products or tobacco substitutes by chemical substances
- A24B15/30—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances
- A24B15/302—Treatment of tobacco products or tobacco substitutes by chemical substances by organic substances by natural substances obtained from animals or plants
- A24B15/303—Plant extracts other than tobacco
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
- A24B3/12—Steaming, curing, or flavouring tobacco
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B9/00—Essential oils; Perfumes
- C11B9/02—Recovery or refining of essential oils from raw materials
- C11B9/025—Recovery by solvent extraction
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- C11B9/00—Essential oils; Perfumes
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Abstract
The invention provides a production method of fermented fructus crataegi flavor. The production method includes the steps of firstly, mixing fructus crataegi powder with water according to mass ratio of 1:(10-40), extracting at 60-75 DEG C for 3-5 hours, performing suction filtration, repeatedly extracting filter residues for 2-3 times, combining filtrate, and sterilizing; secondly, performing activated culture on Bacillus methylotrophicus J4-1 to obtain Bacillus methylotrophicus activated liquid; thirdly, fermenting, to be more specific, inoculating the Bacillus methylotrophicus activated liquid into the combined fructus crataegi filtrate obtained in the first step according to the volume ratio of 5-20% to form fermentation broth; fourthly, performing vacuum concentration on the fermentation broth obtained in the third step at 55-65 DEG C until relative density is 1.2-1.3, performing precipitation on the concentrated fermentation broth and 95% ethanol according to the mass ratio of 1:(3-4) overnight, then performing suction filtration, and performing vacuum distillation on filtrate to remove solvent so as to obtain rufous extract, namely the fermented fructus crataegi flavor. The fermented fructus crataegi flavor can increase the fragrance of reconstituted tobacco, lower stimulation and reduce offensive odor.
Description
Technical field
The present invention relates to cigarette additive technical field, specifically a kind of preparation method of fermentation haw thorn spices and its should
With.
Background technology
Reconstituted tobacco, mainly adds adhesive and other additives etc. to form by offal, fragment, offal or discarded tobacco leaf.
Reconstituted tobacco has that miscellaneous gas weight, excitant be big and mouthfeel is uncomfortable etc. and inhales taste defect, have impact on reconstituted tobacco in cigarette product
Using effect and addition.Domestic common practice is to be improved using the method for additive, applies more adding at present
Plus agent is tobacco, natural plant kind extract, these extracts are typically obtained by water, alcohol extracting or molecular clock, after interpolation
Improvement limitation to cigarette quality, the defect such as still irritant big, miscellaneous gas is many.In order that reconstituted tobacco outward appearance, product of smokeing panel test
Matter, closer to sound tobacco, generally also needs to reconstituted tobacco is carried out perfuming process, and the reconstituted tobacco that prior art produces
And its wrap up in clothing also deposit produce in combustion more xylon gas, the peppery sense of thorn and the problems such as affect cigarette smoke pure smell.
Hawthorn (Crataegus pinnatifida Bunge), also known as fruit, large-fruited Chinese hawthorn, rose family May, drupe in mountain
Class fruit, caryoplasm is hard, and pulp is thin, and taste is micro- sour and astringent.Up to the present, find from hawthorn (pulp, core and leaf) and separation obtains
Material have kind more than 150, wherein mainly have flavonoids, oligomeric flavanoids, organic acid, in addition also contain triterpenes, steroid
With organic amine etc., the forulic acid wherein containing is the microorganism conversion precursor substance of vanillic aldehyde.Natural colouring matter in hawthorn is
The tree peony anthocyanins such as arrow asterin -3- galactoside, the disaccharide of cyanidin or three glucosides (content is 19.6%).Mountain
The pharmacological action of short, bristly hair or beard has：Aid digestion, step-down, reducing blood lipid, anti-oxidant, cardiac stimulant, suppression platelet aggregation, immunological regulation, antibacterial, suppression
The effects such as system distortion, anti-cancer, protection retina.Due to mountain, to pick up acid content with the fingers high, and rich platform pectin, tannin, fruit texture closely, really
Core is hard and proportion is larger, brings larger difficulty to the deep processing of hawthorn.For many years, mountain is picked up processing with the fingers and is confined to always do
Product, sugar prod, beverage product and containerized products aspect, such as mountain pick up sugarcoated haws with the fingers, cake is picked up on haw flakes, mountain with the fingers, haw sheet, haw pulp,
It is more common that the products such as preserved fruit, haw juice and canned haw are picked up on mountain with the fingers, and difficult to form is extensive, industrialization, multiscale, many product
The production planted, hinders mountain and picks up the process change of this China peculiar preciousness fruit resource and comprehensive utilization with the fingers.In addition, picking up with the fingers to mountain
The extraction of flavones ingredient and research are also increasingly being deepened.In addition, hawthorn also contains abundant haematochrome.In order to fully open
Send out and utilize natural resources, extract the bioactive ingredients such as natural food colour from hawthorn, be that China mountain is picked up the profound level of fruit with the fingers and added
Work, the added value that improves open new approach, the economic benefit of raising haw products and social benefit.
And in cigarette product, the macromolecular substances such as the cellulose of excess, protein can give flue gas spine and burning heat sensation
And produce to choke and cough.Cellulose can be generated glucose, compound sugar etc. by biology enzyme directionally hydrolyzing, can effectively reduce the stimulation of cigarette
Property, mitigate miscellaneous gas and improve suction taste, but sugar excessively also can make tarry matters in smoke amount increase.And microorganism can produce in breeding
The huge high activity enzyme system of life, such as polysaccharide hydrolysis enzyme, protease, esterification enzyme, redox enzymes and lyases etc.,
Under the synergy of complicated metabolism in enzymatic catalysis, chemical action and microbial body, decomposed for raw material with hawthorn, drop
The effects such as solution, oxidation, reduction, polymerization, coupling, conversion, form complicated low molecular compound, including various fragrance chemical combination
Thing, such as alcohols, aldehydes, ketone, acids, lipid, phenols, pyran, pyridines and terpenes etc., these aroma substances are undoubtedly
It is tobacco or the aroma component of the additive for tobacco.
In prior art, with hawthorn extract as substrate, methyl alcohol bacillus are eaten with aroma former
Methylotrophicus VJ4-1 ferments, and prepares fermentation haw thorn spices and its in reconstituted tobacco and wraps up in application in clothing, improves
The mouthfeel making tobacco leaf inhales taste, and makes outward appearance close to sound tobacco, and there is not been reported.
Content of the invention
The present invention is directed to the deficiencies in the prior art, provides a kind of preparation method of fermentation haw thorn spices, and in reconstituted tobacco
Applied with wrapping up in clothing, reconstituted tobacco aroma quality can be improved, reduce stimulation, reduce miscellaneous gas, and reconstituted tobacco can be made and wrap up in clothing more
Appearance luster close to sound tobacco.
The present invention provides a kind of preparation method of fermentation haw thorn spices, and it utilizes aroma former to eat methyl alcohol bacillus VJ4-1
Fermentation haw thorn spices, comprises the following steps：
1) by hawthorn powder and water in mass ratio 1:10～40 mixing, 60～75 DEG C extract 3～5 hours, suction filtration, by filter residue weight
Extract 2～3 times again, merging filtrate, sterilizing；
2) bacterial strain activation：Methyl alcohol bacillus VJ4-1 activation culture will be eaten, obtain eating methyl alcohol bacillus activating solution；Bacterium
Strain activation condition be：25～35 DEG C, under the conditions of pH4～7,100～150r/min concussion and cultivate 24～72 hours, described product is fragrant
Bacterium food methyl alcohol bacillus (Bacillus methylotrophicus) VJ4-1 is deposited in Wuhan University's Chinese Typical Representative culture
Collection, preserving number is CCTCC NO:M 2012004, preservation date：On January 11st, 2012；
3) ferment：By step 2) in the activation bacterium solution of gained 5～20% be inoculated into above-mentioned steps 1 by volume) in mountain
Form zymotic fluid, the tinning amount of above-mentioned zymotic fluid is 20～40%, and postvaccinal fermentation culture conditions are with alive in short, bristly hair or beard merging filtrate
Change condition of culture；Incubation time is 1～7 day；
4) by step 3) in the zymotic fluid of gained be evaporated to relative density 1.2～1.3, with 95% ethanol in mass ratio
1：Suction filtration after 3～4 precipitates overnight, filtrate decompression is distilled off solvent, and prepared dark reddish brown medicinal extract is fermentation haw thorn spices.
Further, described step 4) in decompression vacuum distillation temperature be 55～65 DEG C.
The present invention also provides a kind of application in reconstituted tobacco for fermentation haw thorn spices of said method preparation, described fermentation
It is 0.05～0.3% that hawthorn spices adds the mass ratio in reconstituted tobacco.
The present invention also provides the application that a kind of fermentation haw thorn spices of said method preparation is wrapped up in clothing in reconstituted tobacco, described
It is 0.05～0.1% that fermentation haw thorn spices adds the mass ratio that reconstituted tobacco is wrapped up in clothing.
The aroma former fermentation haw thorn spices using GC/MS prepared by the present invention is (hereinafter referred to as right with non-fermentation haw thorn spices
According to) carry out volatility aroma component GC/MS comparative analysis.
(1) Simultaneous distillation-extraction
5g fermentation haw thorn spices or comparison are loaded in round-bottomed flask, add 400mL water, be heated to seething with excitement with electric jacket,
Add the dichloromethane of 200mL, 60 DEG C of water-bath boilings in the round-bottomed flask of opposite side, treat that at middle separating tube, layering in liquid level
Timing extraction 2h afterwards.After extraction terminates, add anhydrous sodium sulfate in dichloromethane extract, overnight, be filtered and concentrated to
1mL, concentrate is used for carrying out GC/MS analysis.
(2) GC/MS analysis：HP-5MS (50m × 0.25mm × 0.25 μm) capillary column；Injector temperature：250℃；Shunting
Ratio 20:1；Carrier gas：He, 1mL/min；Heating schedule：From 50 DEG C (1min), 250 DEG C (5min) are raised to 5 DEG C/min；Transmission line temperature
Degree：250℃；Ion gun：EI source；Electron energy：70eV；Sweep limits：50～550amu；Compose storehouse using WILEY and MINILAB
Enter line retrieval.
(3) volatile ingredient comparative analysis
Analysis of aroma components is carried out to the aroma former fermentation haw thorn spices of the present invention and its comparison by GC/MS, from table 1
As can be seen that (1) fermentation haw thorn spices identifies altogether 58 kinds of aroma volatile, non-fermentation haw thorn comparison medicinal extract sample mirror
Make 34 kinds of volatile ingredients；(2) fermentation haw thorn spices increases 35 kinds of aroma volatile newly, mainly includes caproic acid, β-bay
Alkene, hexyl acetate, n-hexyl butyrate, terpinol, vanillic alcohol, vanillic aldehyde, dihydroactinidiolide, benzyl carbinol and other alcohols, alkene
Class, furans, ketone etc., wherein caproic acid are lower fatty acid, VFA, favourable to the flue gas of cigarette, make smoke alcohol
With；Beta-myrcene has pleasant, light balsamic odour；Hexyl acetate has fruital gas；N-hexyl butyrate is in blue or green fragrant, really
The mixed fruit fragrance fragrant, ester is fragrant, vegetables are fragrant and strong and pineapple perfume (or spice) are hidden；Terpinol, like pine-needle aroma, increases blue or green in flue gas
Perfume (or spice), plays an important role in cigarette fragrance；Vanillic alcohol has that fragrant and sweet, cream, phenol is fragrant, vanilla and coconut fragrance, and vanillic aldehyde has
There is strong milk fragrance, can be evaporate in flue gas by high temperature, increase the vanilla fragrance of cigarette smoke, coordinate cigarette perfume, increase cigarette
Pyrans in gas, pyridines flavor matter, improve the flavor component of cigarette smoke, are effectively improved the aesthetic quality of cigarette, may
It is forulic acid in hawthorn through microbial enzyme system catalyzed conversion；Dihydroactinidiolide, with cumarin sample fragrance, and
There is Moschus sample breath；Benzyl carbinol, has soft, happy and lasting rose fragrance；(3) in fermentation haw thorn spices, hexanal, chaff
The content of the compositions such as aldehyde, certain herbaceous plants with big flowers aldehyde, ethyl palmitate has different degrees of raising.Wherein hexanal is in raw grease and green grass gas and apple
Fruit flavor；Furfural has almond perfumed ribbon burnt odor；Ethyl palmitate is senior saturated fatty acid, perfume, the fruit rank of nobility and cream in faint wax
Fragrance, has the effect of soft fragrance in cigarette smoke；(4), after fermenting, some compositions in hawthorn disappear or content reduces,
As 3-M2BOL, 2,3- pentanedione, chloromethyl acetate, acetal, tridecanoic acid, phthalic acid isobutyl ester, Asia
Oleic acid, ethyl linoleate, phthalic acid list (2- ethylhexyl) ester etc., its Linoleic acid, ethyl linoleate belong to unsaturated
Acid, unsaturated acid ester, phthalic acid isobutyl ester, phthalic acid list (2- ethylhexyl) ester belong to destructive stimulus composition,
Easily form intense irritation and miscellaneous gas material in thermal cracking processes, increase flue gas to the excitant of throat and harsh feeling, right
Cigarette odor-absorbing has detrimental effect, significantly reduces or disappear it may be possible to because through microbial enzyme after their content is fermented
System acts on and decomposes, degrades, converts, it is advantageously possible for the raising of cigarette quality.In a word, from volatilization, semi-volatility component
Change is seen, through eating the hawthorn spices of methyl alcohol bacillus methylotrophicus VJ4-1 transformation fermentation in volume
Perfuming effect in cigarette may be better than unfermentable hawthorn extract.
Table 1. aroma former fermentation haw thorn spices is not fermented with it volatile ingredient GC/MS comparative analysis compareing
In table 1, symbol "/" represents and does not detect；Inside it is designated as phenylethyl propionate.
Through reconstituted tobacco and wrap up in the test of smokeing panel test of clothing perfuming and show, all these fragrance blend together imparting fermentation haw thorn
Spices and flue gas are coordinated, sour-sweet milk fragrance, increased the soft sense of flue gas, reduction stimulates, reduce miscellaneous gas, improvement is inhaled taste, and made
Make tobacco leaf and to wrap up in clothing color more natural uniformly, close to sound tobacco.
Microorganism can produce huge high activity enzyme system in breeding, such as polysaccharide hydrolysis enzyme, protease, esterification
Enzyme, redox enzymes and lyases etc., the collaborative work of complicated metabolism in enzymatic catalysis, chemical action and microbial body
With under, think that raw material such as is decomposed, degrades, aoxidizing, reducing, being polymerized, being coupled, converting at the effect, formed complicated degraded
Compound, including various perfume compounds, such as alcohols, aldehydes, ketone, acids, lipid, phenols, pyran etc., these fragrance
Material is also undoubtedly tobacco or the aroma component of the additive for tobacco.
The invention has the beneficial effects as follows：
1) product vanillic aldehyde bacterial strain food methyl alcohol bacillus (Bacillus methylotrophicus) of present invention preparation
VJ4-1 fermentation haw thorn spices, can be used for reconstituted tobacco and the perfuming wrapping up in clothing.During for reconstituted tobacco, flue gas can be coordinated, make fragrance
Matter is lifted, and fragrance is more rich and carries significantly sour-sweet milk fragrance, and also have reduction stimulates simultaneously, reduces the effect of miscellaneous gas；With
When cigarette paper, the quality of cigarette paper can be lifted so as to assume the high-quality pipe tobacco yellow at natural one's own department or unit and tone is naturally uniform, energy
Cover xylon gas produced by cigarette paper burning well, pierce peppery sense.
2) adopt the fermentation haw thorn spices of present invention preparation, odourant ingredients content is high, and product yield is high, with short production cycle,
Process conditions are gentle, and raw material used has the advantages that natural environmental-protective is harmless, tool step compared with producing the single spices of vanillic aldehyde
Suddenly simplicity can reduce purifying cost, fragrance composite natral, has larger commercial Application to convert prospect.
Specific embodiment
Be set forth below a part of embodiment the relevant technical problem of the present invention is described in further detail it is necessary to
This points out that specific examples below simply further illustrates to the present invention, does not represent limiting the scope of the invention.Its
Some nonessential modifications that other people make according to the present invention and adjusting, still fall within protection scope of the present invention.
Embodiment 1
A kind of preparation method of fermentation haw thorn spices, comprises the steps：
1) by hawthorn powder and water in mass ratio 1:20 mixing, 60 DEG C extract 3 hours, suction filtration, filter residue is repeated to extract 2 times,
Merging filtrate, sterilizing；
2) bacterial strain activation：Methyl alcohol bacillus VJ4-1 activation culture will be eaten, obtain eating methyl alcohol bacillus activating solution；Bacterium
Strain activation condition be：25 DEG C, under the conditions of pH4.5,100r/min concussion and cultivate 24 hours；
3) ferment：By step 2) in the activation bacterium solution of gained 5% be inoculated into above-mentioned steps 1 by volume) in hawthorn close
And in filtrate, forming zymotic fluid, the tinning amount of above-mentioned zymotic fluid is 20%, and postvaccinal fermentation culture conditions are with activation culture bar
Part；Incubation time is 2 days；
4) by step 3) in the zymotic fluid of gained be evaporated to relative density 1.2～1.3, with 95% ethanol in mass ratio
1：Suction filtration after 3～4 precipitates overnight, filtrate decompression is distilled off solvent, and prepared dark reddish brown medicinal extract is fermentation haw thorn spices, phase
It is 1.0144 to density.
It is 0.05% that this fermentation haw thorn spices adds the mass ratio in reconstituted tobacco, can soft flue gas, abundant fragrance, minimizing
Stimulate and miscellaneous gas, make reconstituted tobacco more glossy, closer to natural yellow tobacco leaves.
It is 0.05% that this fermentation haw thorn spices adds the mass ratio wrapped up in clothing, can be lifted aroma quality, increase sour-sweet milk fragrance,
Reduce stimulate, pleasant impression clean, make to wrap up in clothing and manifest the yellowish-brown close to tobacco leaf.
Embodiment 2
A kind of preparation method of fermentation haw thorn spices, comprises the steps：
1) by hawthorn powder and water in mass ratio 1:15 mixing, 65 DEG C extract 4 hours, suction filtration, filter residue is repeated to extract 3 times,
Merging filtrate, sterilizing；
2) bacterial strain activation：Methyl alcohol bacillus VJ4-1 activation culture will be eaten, obtain eating methyl alcohol bacillus activating solution；Bacterium
Strain activation condition be：30 DEG C, under the conditions of pH5,110r/min concussion and cultivate 48 hours；
3) ferment：By step 2) in the activation bacterium solution of gained 10% be inoculated into above-mentioned steps 1 by volume) in hawthorn close
And in filtrate, forming zymotic fluid, the tinning amount of above-mentioned zymotic fluid is 30%, and postvaccinal fermentation culture conditions are with activation culture bar
Part；Incubation time is 3 days；
4) by step 3) in the zymotic fluid of gained be evaporated to relative density 1.2～1.3, with 95% ethanol in mass ratio
1：Suction filtration after 3～4 precipitates overnight, filtrate decompression is distilled off solvent, and prepared dark reddish brown medicinal extract is fermentation haw thorn spices, phase
It is 1.0201 to density.
This fermentation haw thorn spices mass ratio in reconstituted tobacco is 0.1%, and flue gas is coordinated, increased sour-sweet milk, fall
Low stimulation, color is more natural, glossy.
Embodiment 3
A kind of preparation method of fermentation haw thorn spices, comprises the steps：
1) by hawthorn powder and water in mass ratio 1:40 mixing, 70 DEG C extract 5 hours, suction filtration, filter residue is repeated to extract 3 times,
Merging filtrate, sterilizing；
2) bacterial strain activation：Methyl alcohol bacillus VJ4-1 activation culture will be eaten, obtain eating methyl alcohol bacillus activating solution；Bacterium
Strain activation condition be：35 DEG C, under the conditions of pH6,130r/min concussion and cultivate 72 hours；
3) ferment：By step 2) in the activation bacterium solution of gained 20% be inoculated into above-mentioned steps 1 by volume) in hawthorn close
And in filtrate, forming zymotic fluid, the tinning amount of above-mentioned zymotic fluid is 40%, and postvaccinal fermentation culture conditions are with activation culture bar
Part；Incubation time is 5 days；
4) by step 3) in the zymotic fluid of gained be evaporated to relative density 1.2～1.3, with 95% ethanol in mass ratio
1：Suction filtration after 3～4 precipitates overnight, filtrate decompression is distilled off solvent, and prepared dark reddish brown medicinal extract is fermentation haw thorn spices, phase
It is 1.1417 to density.
It is 0.3% that this fermentation haw thorn spices adds the mass ratio in reconstituted tobacco, relatively can coordinate with flue gas, increase fragrance,
Reduce miscellaneous gas, improve mouthfeel.
Embodiment 4 is smoked panel test experiment
By the fermentation haw thorn spices of preparation respectively by the mass ratio with reconstituted tobacco be 0.3%, 0.1%, 0.05% ratio
Example, the ratio that non-fermentation haw thorn extract medicinal extract (hereinafter referred to as compareing) is 0.05% in the mass ratio with reconstituted tobacco, respectively
It is added in reconstituted tobacco, is designated as 1#, 2#, 3#, control sample successively, is smoked panel test, smoke panel test according to GB GB 5606.4-
1996 are carried out.
Application in reconstituted tobacco for the table 2. fermentation haw thorn spices
According to upper table 2, fermentation haw thorn spices of the present invention, add reconstituted tobacco in carry out perfuming ratio be 0.05%～
When 0.3%, good with flue gas harmony, there is the sour-sweet milk of feature, fragrance enriches, and flue gas is soft, fine and smooth, stimulate and reduce, miscellaneous gas subtracts
Less, inhale taste to improve, and reconstituted tobacco can be improved and wrap up in clothing so as to appearance color is naturally uniform, close to sound tobacco.
The above, the only specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, and any
Belong to those skilled in the art the invention discloses technical scope in, the change or replacement that can readily occur in, all answer
It is included within the scope of the present invention.Therefore, protection scope of the present invention should be defined by scope of the claims.
Claims (4)
1. a kind of preparation method of fermentation haw thorn spices it is characterised in that：Including
1) by hawthorn powder and water in mass ratio 1:10～40 mixing, 60～75 DEG C extract 3～5 hours, suction filtration, filter residue is repeated to carry
Take 2～3 times, merging filtrate, sterilizing；
2) bacterial strain activation：Methyl alcohol bacillus VJ4-1 activation culture will be eaten, obtain eating methyl alcohol bacillus activating solution；Bacterial strain is lived
Change condition is：25～35 DEG C, under the conditions of pH4～7,100～150r/min concussion and cultivate 24～72 hours, described aroma former food
Methyl alcohol bacillus (Bacillus methylotrophicus) VJ4-1 is deposited in Wuhan University's China typical culture collection
Center, preserving number is CCTCC NO:M 2012004, preservation date：On January 11st, 2012；
3) ferment：By step 2) in the activation bacterium solution of gained 5～20% be inoculated into above-mentioned steps 1 by volume) in hawthorn close
And in filtrate, forming zymotic fluid, the tinning amount of above-mentioned zymotic fluid is 20～40%, and postvaccinal fermentation culture conditions are same to activate training
Foster condition, incubation time is 1～7 day；
4) by step 3) in the zymotic fluid of gained be evaporated to relative density 1.2～1.3, with 95% ethanol in mass ratio 1：3
Suction filtration after～4 precipitates overnight, filtrate decompression is distilled off solvent, and prepared dark reddish brown medicinal extract is fermentation haw thorn spices.
2. fermentation haw thorn spices as claimed in claim 1 preparation method it is characterised in that：Described step 4) middle decompression decompression
The temperature of distillation is 55～65 DEG C.
3. application in reconstituted tobacco for a kind of fermentation haw thorn spices of the method for claim 1 preparation, described fermentation
It is 0.05～0.3% that hawthorn spices adds the mass ratio in reconstituted tobacco.
4. a kind of fermentation haw thorn spices of the method for claim 1 preparation wraps up in the application in clothing in reconstituted tobacco, described
It is 0.05～0.1% that fermentation haw thorn spices adds the mass ratio that reconstituted tobacco is wrapped up in clothing.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610109532.8A CN105754720A (en) | 2016-02-29 | 2016-02-29 | Preparation method and application of fermented hawthorn pigment spice |
| CN2016101095328 | 2016-02-29 |
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| CN106434756A true CN106434756A (en) | 2017-02-22 |
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| CN201610109532.8A Withdrawn CN105754720A (en) | 2016-02-29 | 2016-02-29 | Preparation method and application of fermented hawthorn pigment spice |
| CN201610586318.1A Pending CN106434756A (en) | 2016-02-29 | 2016-07-22 | Production method and application of fermented fructus crataegi flavor |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109402101A (en) * | 2018-11-09 | 2019-03-01 | 湖北中烟工业有限责任公司 | A kind of absorption fermentation preparation of Sun cured tobacco extract and application |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102204715A (en) * | 2011-01-29 | 2011-10-05 | 广州市澳键丰泽生物科技有限公司 | Formula of hawthorn-extract-containing tobacco casing flavor |
| CN102851229A (en) * | 2012-04-18 | 2013-01-02 | 湖北中烟工业有限责任公司 | Preparation method and application of Bacillus methylotrophicus and tobacco angelica sinensis extract thereof |
| CN102851228A (en) * | 2012-04-18 | 2013-01-02 | 湖北中烟工业有限责任公司 | Preparation method and application of Bacillus methylotrophicus and tobacco spine date seed extract thereof |
-
2016
- 2016-02-29 CN CN201610109532.8A patent/CN105754720A/en not_active Withdrawn
- 2016-07-22 CN CN201610586318.1A patent/CN106434756A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102204715A (en) * | 2011-01-29 | 2011-10-05 | 广州市澳键丰泽生物科技有限公司 | Formula of hawthorn-extract-containing tobacco casing flavor |
| CN102851229A (en) * | 2012-04-18 | 2013-01-02 | 湖北中烟工业有限责任公司 | Preparation method and application of Bacillus methylotrophicus and tobacco angelica sinensis extract thereof |
| CN102851228A (en) * | 2012-04-18 | 2013-01-02 | 湖北中烟工业有限责任公司 | Preparation method and application of Bacillus methylotrophicus and tobacco spine date seed extract thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109402101A (en) * | 2018-11-09 | 2019-03-01 | 湖北中烟工业有限责任公司 | A kind of absorption fermentation preparation of Sun cured tobacco extract and application |
| CN109402101B (en) * | 2018-11-09 | 2021-05-18 | 湖北中烟工业有限责任公司 | Adsorption fermentation preparation method and application of sun-cured yellow tobacco extract |
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| Publication number | Publication date |
|---|---|
| CN105754720A (en) | 2016-07-13 |
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