CN106418459A - Method for preparing composite antioxidant from caulis dendrobii - Google Patents
Method for preparing composite antioxidant from caulis dendrobii Download PDFInfo
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- CN106418459A CN106418459A CN201610800412.2A CN201610800412A CN106418459A CN 106418459 A CN106418459 A CN 106418459A CN 201610800412 A CN201610800412 A CN 201610800412A CN 106418459 A CN106418459 A CN 106418459A
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- parts
- aqueous phase
- composite antioxidant
- stem
- take
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- 230000003078 antioxidant effect Effects 0.000 title claims abstract description 29
- 239000003963 antioxidant agent Substances 0.000 title claims abstract description 27
- 239000002131 composite material Substances 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims abstract description 13
- 239000008346 aqueous phase Substances 0.000 claims abstract description 18
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 12
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000003995 emulsifying agent Substances 0.000 claims abstract description 8
- 239000000839 emulsion Substances 0.000 claims abstract description 7
- 150000003839 salts Chemical class 0.000 claims abstract description 7
- 238000010008 shearing Methods 0.000 claims abstract description 7
- 235000011187 glycerol Nutrition 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 239000000843 powder Substances 0.000 claims abstract description 6
- 239000007787 solid Substances 0.000 claims abstract description 6
- 238000003756 stirring Methods 0.000 claims abstract description 6
- 239000000919 ceramic Substances 0.000 claims abstract description 5
- 239000012528 membrane Substances 0.000 claims abstract description 5
- 239000000284 extract Substances 0.000 claims description 18
- 241001523681 Dendrobium Species 0.000 claims description 17
- 240000004638 Dendrobium nobile Species 0.000 claims description 17
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- 239000000470 constituent Substances 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 238000010438 heat treatment Methods 0.000 claims description 5
- 239000012467 final product Substances 0.000 claims description 4
- 238000007654 immersion Methods 0.000 claims description 4
- NPTLAYTZMHJJDP-KTKRTIGZSA-N [3-[3-[3-[3-[3-[3-[3-[3-[3-(2,3-dihydroxypropoxy)-2-hydroxypropoxy]-2-hydroxypropoxy]-2-hydroxypropoxy]-2-hydroxypropoxy]-2-hydroxypropoxy]-2-hydroxypropoxy]-2-hydroxypropoxy]-2-hydroxypropoxy]-2-hydroxypropyl] (z)-octadec-9-enoate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OCC(O)COCC(O)COCC(O)COCC(O)COCC(O)COCC(O)COCC(O)COCC(O)COCC(O)COCC(O)CO NPTLAYTZMHJJDP-KTKRTIGZSA-N 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 238000004945 emulsification Methods 0.000 claims description 3
- 150000001875 compounds Chemical class 0.000 claims description 2
- 235000006708 antioxidants Nutrition 0.000 abstract description 23
- 206010011224 Cough Diseases 0.000 abstract description 3
- 210000004072 lung Anatomy 0.000 abstract description 3
- 210000002784 stomach Anatomy 0.000 abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 3
- 239000000243 solution Substances 0.000 abstract 3
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 abstract 2
- 238000001914 filtration Methods 0.000 abstract 2
- 229930003427 Vitamin E Natural products 0.000 abstract 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 abstract 1
- 235000016709 nutrition Nutrition 0.000 abstract 1
- 239000002245 particle Substances 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
- 239000012266 salt solution Substances 0.000 abstract 1
- 235000019165 vitamin E Nutrition 0.000 abstract 1
- 229940046009 vitamin E Drugs 0.000 abstract 1
- 239000011709 vitamin E Substances 0.000 abstract 1
- 239000000047 product Substances 0.000 description 9
- 230000003647 oxidation Effects 0.000 description 6
- 238000007254 oxidation reaction Methods 0.000 description 6
- 150000004676 glycans Chemical class 0.000 description 5
- 229920001282 polysaccharide Polymers 0.000 description 5
- 239000005017 polysaccharide Substances 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 4
- 235000013361 beverage Nutrition 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 230000003064 anti-oxidating effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 235000020124 milk-based beverage Nutrition 0.000 description 2
- WBYWAXJHAXSJNI-VOTSOKGWSA-M .beta-Phenylacrylic acid Natural products [O-]C(=O)\C=C\C1=CC=CC=C1 WBYWAXJHAXSJNI-VOTSOKGWSA-M 0.000 description 1
- 229930016911 cinnamic acid Natural products 0.000 description 1
- 235000013985 cinnamic acid Nutrition 0.000 description 1
- -1 cinnamic acid ester Chemical class 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 235000019261 food antioxidant Nutrition 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- WBYWAXJHAXSJNI-UHFFFAOYSA-N methyl p-hydroxycinnamate Natural products OC(=O)C=CC1=CC=CC=C1 WBYWAXJHAXSJNI-UHFFFAOYSA-N 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 229910052573 porcelain Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a method for preparing a composite antioxidant from caulis dendrobii. The method comprises the steps that 10 parts of dry caulis dendrobii powder which is crushed to 50 mesh is taken and put into 20-40 parts of a 70% ethanol solution to be soaked for 30 min, filtration is conducted, a solid component is taken and added into 50-100 parts of a salt solution containing 1%-2% of salt to be soaked for 1-3 h at the temperature of 30 DEG C to 50 DEG C, filtration is conducted, a liquid component is taken, filtered with a ceramic membrane and then concentrated until Brix reaches 20%, and a caulis dendrobii extracting solution is obtained; 1-3 parts of an emulsifier is taken and heated to be dissolved into 60-80 parts of glycerin, then 10-30 parts of the caulis dendrobii extracting solution is added, stirring continues, the emulsifier is dissolved uniformly, and an aqueous phase is obtained; the aqueous phase is sheared at the revolving speed of 5,000-10,000 rpm, 7-9 parts of natural vitamin E is weighed and slowly added into the aqueous phase, shearing is conducted for 15-20 min until the average particle size of emulsion ranges from 0.2 micrometer to 0.6 micrometer, and then the composite antioxidant is obtained. The composite antioxidant prepared through the method has the very high antioxidant capacity, is soluble in water, convenient to use and wide in application range and has multiple nutritional and health-care functions of benefiting the stomach, promoting essence, nourishing yin, clearing heat, moistening the lung, relieving coughing and the like of caulis dendrobii.
Description
Technical field
The present invention relates to a kind of preparation method of antioxidant, prepare composite antioxidant particularly to one kind using the stem of noble dendrobium
Method.
Background technology
The stem of noble dendrobium is the perennial herbaceous plant that grows nonparasitically upon another plant, and is distributed in Southwestern China and south of the River each province.Dendrobium polysaccharide is contained in the stem of noble dendrobium
Etc. several functions composition, the effects such as there is beneficial stomach production of sperm, nourishing Yin and clearing heat, moisten the lung and relieve the cough.Being used for the stem of noble dendrobium extracting dendrobium polysaccharide more
Prepare health food, product such as such as commercially available iron sheet wind scoop etc..There are some researches show that dendrobium polysaccharide has Partial Antioxidation in recent years
Ability, but the stem of noble dendrobium anti-oxidant aspect product exploitation also in the Primary Study stage.
Natural VE（VE）It is a kind of natural antioxidant, there is certain oxidation resistance, VE is fat-soluble product
Product, water insoluble, it is usually used in grease preventing Oxidation of Fat and Oils.At present, there is research that fat-soluble VE is passed through shearing homogeneous means straight
Connect interpolation to water-soluble food such as milk beverage, but this technique not only increases the equipment investment of beverage processing, and disperse energy
Power is limited, is easily caused beverage systems unstable.Also research is had to make natural VE by the technological means emulsifying or embed water-soluble
Property product, add in water-soluble food, but the method need to add substantial amounts of emulsifying agent, not only affect product local flavor, and
And be embedded with VE, oxidation resistance is restricted.
Content of the invention
For overcoming the deficiencies in the prior art, it is an object of the invention to provide one kind prepares composite antioxidant using the stem of noble dendrobium
Method, the antioxidant product preparing is stable, and oxidation resistance is strong.
For achieving the above object, the method that composite antioxidant prepared by the utilization stem of noble dendrobium involved in the present invention includes following system
Standby step：
（1）The stem of noble dendrobium extracts：In mass, take drying and crushing to 10 parts of the dendrobe powder of 50 mesh, be placed in 20-40 part 70% ethanol solution
Middle immersion 30min, then filters to take solid constituent, adds 50-100 part to contain the saline solution of 1%-2% salt, soaks in 30-50 DEG C
1-3h, filters to take liquid component, be concentrated into after ceramic membrane filter Brix reach 20% dendrobium extract；
（2）Prepare composite antioxidant：Take emulsifying agent 1-3 part, heating for dissolving, in 60-80 part glycerine, is subsequently adding 10-30 part
Dendrobium extract, continuation stirring and dissolving is uniform to obtain aqueous phase；5000-10000rpm rotating speed down cut aqueous phase, and weigh natural dimension life
Plain E 7-9 part, is slowly added in aqueous phase, and shearing 15-20min, to 0.2 μm -0.6 μm of emulsion average grain diameter, obtains final product compound antioxygen
Agent.
Wherein, described emulsifying agent is ten Plurol Oleique CC497s, ten polyglycereol monolaurates or ten polyglycereol February
In cinnamic acid ester.
The preparation method of the food antioxidant of the present invention has the advantage that：
（1）The antioxidant that the present invention prepares has very strong oxidation resistance, and application experiment shows, its oxidation resistance
1.5 times of equal quality VE can be reached, be 4 times of equal quality dendrobium polysaccharide, not only not because emulsification and embedding have impact on
VE and the antioxidant effect of Herba Dendrobii extract, serve on the contrary and significantly work in coordination with enhancing effect.High anti-oxidation activity can reduce
Consumption, reduces the production cost of client；
（2）The antioxidant that the present invention prepares is water solubility, and easy to use, application is wide；
（3）The composite antioxidant preparing by preparation method of the present invention, in addition to antioxidant effect, also has the stem of noble dendrobium
Multiple alimentary health-care functions, as beneficial stomach production of sperm, nourishing Yin and clearing heat, moisten the lung and relieve the cough；
（4）The preparation method of the composite antioxidant that the present invention provides, process is simple, without complicated technologies such as homogeneous, equipment is thrown
Enter and significantly reduce with energy consumption.In addition, the present invention is in stem of noble dendrobium extraction step, carried out with the saline solution containing 1-2% salt extracting system
Standby dendrobium extract, obtains dendrobium extract and has promotion emulsification function, reduce the addition of emulsifying agent in finished product, only account for whole
The 1-3% of individual product, and tradition needs emulsifying agent addition about 5% about in the emulsion that homogeneous could be stable.
Specific embodiment
Below in conjunction with instantiation, technical scheme is described further.
Embodiment 1
（1）The stem of noble dendrobium extracts：Take drying and crushing to dendrobe powder 500g of 50 mesh, be placed in 1000g 70% ethanol solution and soak
30min, then filters to take solid constituent, adds 2500g to contain the saline solution of 2% salt, soaks 3h in 30 DEG C, filters to take liquid
Point, be concentrated into after ceramic membrane filter Brix reach 20% dendrobium extract；
（2）Prepare composite antioxidant：Take ten polyglycereol monolaurate 30g, heating for dissolving, in 800g glycerine, is subsequently adding
100g dendrobium extract, continuation stirring and dissolving is uniform to obtain aqueous phase；10000rpm rotating speed down cut aqueous phase, and weigh natural complex
E 70g, is slowly added in aqueous phase, and shearing 15min is 0.28 μm to emulsion average grain diameter, obtains composite antioxidant.
Embodiment 2
（1）The stem of noble dendrobium extracts：Take drying and crushing to dendrobe powder 100g of 50 mesh, be placed in immersion 30min in 400g 70% ethanol solution,
Then filter to take solid constituent, add 1000g to contain the saline solution of 1% salt, soak 1h in 50 DEG C, filter to take liquid component, through pottery
Be concentrated into after porcelain membrane filtration Brix reach 20% dendrobium extract；
（2）Prepare composite antioxidant：Take ten Plurol Oleique CC497 10g, heating for dissolving, in 600g glycerine, is subsequently adding
300g dendrobium extract, continuation stirring and dissolving is uniform to obtain aqueous phase；5000rpm rotating speed down cut aqueous phase, and weigh natural VE
90g, is slowly added in aqueous phase, and shearing 20min, to 0.56 μm of emulsion average grain diameter, obtains final product composite antioxidant.
Embodiment 3
（1）The stem of noble dendrobium extracts：Take drying and crushing to dendrobe powder 100g of 50 mesh, be placed in immersion 30min in 300g 70% ethanol solution,
Then filter to take solid constituent, add 800g to contain the saline solution of 1.5% salt, soak 2h in 40 DEG C, filter to take liquid component, warp
Be concentrated into after ceramic membrane filter Brix reach 20% dendrobium extract；
（2）Prepare composite antioxidant：Take ten polyglycereol dilaurate 20g, heating for dissolving, in 700g glycerine, is subsequently adding
200g dendrobium extract, continuation stirring and dissolving is uniform to obtain aqueous phase；8000rpm rotating speed down cut aqueous phase, and weigh natural VE
80g, is slowly added in aqueous phase, and shearing 20min reaches 0.37 μm to emulsion average grain diameter, obtains final product composite antioxidant.
Embodiment 1-3 is applied to protein content with the natural VE of equal quality and equal quality dendrobium polysaccharide
In 1.5% milk beverage, then beverage is stored in 35 DEG C and carries out local flavor contrast, shown in result following table.
Claims (2)
1. prepare the method for composite antioxidant it is characterised in that comprising the following steps using the stem of noble dendrobium:
（1）The stem of noble dendrobium extracts：In mass, take drying and crushing to 10 parts of the dendrobe powder of 50 mesh, be placed in 20-40 part 70% ethanol solution
Middle immersion 30min, then filters to take solid constituent, adds 50-100 part to contain the saline solution of 1%-2% salt, soaks in 30-50 DEG C
1-3h, filters to take liquid component, be concentrated into after ceramic membrane filter Brix reach 20% dendrobium extract；
（2）Prepare composite antioxidant：Take emulsifying agent 1-3 part, heating for dissolving, in 60-80 part glycerine, is subsequently adding 10-30 part
Dendrobium extract, continuation stirring and dissolving is uniform to obtain aqueous phase；5000-10000rpm rotating speed down cut aqueous phase, and weigh natural dimension life
Plain E 7-9 part, is slowly added in aqueous phase, and shearing 15-20min, to 0.2 μm -0.6 μm of emulsion average grain diameter, obtains final product compound antioxygen
Agent.
2. according to claim 1 prepare the method for composite antioxidant it is characterised in that described emulsification using the stem of noble dendrobium
Agent is ten Plurol Oleique CC497s, ten polyglycereol monolaurates or ten polyglycereol dilaurates.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610800412.2A CN106418459A (en) | 2016-09-04 | 2016-09-04 | Method for preparing composite antioxidant from caulis dendrobii |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610800412.2A CN106418459A (en) | 2016-09-04 | 2016-09-04 | Method for preparing composite antioxidant from caulis dendrobii |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106418459A true CN106418459A (en) | 2017-02-22 |
Family
ID=58164653
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610800412.2A Pending CN106418459A (en) | 2016-09-04 | 2016-09-04 | Method for preparing composite antioxidant from caulis dendrobii |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106418459A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104287053A (en) * | 2014-09-30 | 2015-01-21 | 杭州娃哈哈科技有限公司 | Oil-in-water type water-soluble antioxidant compound and preparation method thereof |
| CN105901733A (en) * | 2016-02-22 | 2016-08-31 | 上海交通大学 | Antioxidation application of dendrobe leaf |
-
2016
- 2016-09-04 CN CN201610800412.2A patent/CN106418459A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104287053A (en) * | 2014-09-30 | 2015-01-21 | 杭州娃哈哈科技有限公司 | Oil-in-water type water-soluble antioxidant compound and preparation method thereof |
| CN105901733A (en) * | 2016-02-22 | 2016-08-31 | 上海交通大学 | Antioxidation application of dendrobe leaf |
Non-Patent Citations (2)
| Title |
|---|
| 李志华: "铁皮石斛多糖的提取、分离、纯化及结构分析", 《中国优秀硕士学位论文全文数据库 工程科技I辑》 * |
| 杨建雄: "《生物化学与分子生物学实验技术教程》", 31 March 2014, 科学出版社 * |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170222 |