CN106417387A - Paecilomyces lilacinus oil suspension agent and preparation method - Google Patents
Paecilomyces lilacinus oil suspension agent and preparation method Download PDFInfo
- Publication number
- CN106417387A CN106417387A CN201610841921.XA CN201610841921A CN106417387A CN 106417387 A CN106417387 A CN 106417387A CN 201610841921 A CN201610841921 A CN 201610841921A CN 106417387 A CN106417387 A CN 106417387A
- Authority
- CN
- China
- Prior art keywords
- paecilomyces lilacinus
- oil
- suspending agent
- parts
- paecilomyces
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
- A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
Landscapes
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Dispersion Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Toxicology (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a paecilomyces lilacinus oil suspension agent which comprises, by weight, 4-10 parts of drug active component, 12-20 parts of auxiliary and 70-85 parts of mineral oil. The drug active component is paecilomyces lilacinus. The oil suspension agent is excellent in dispersion in water, disperses cloudily, is stable in property, free of layering and precipitating in the process of storing and low in thermal storage decomposition rate and cost; drug liquid is easy to permeate to a target, so that efficacy is good; the oil suspension agent is low in toxicity, stable in insecticidal effect, safe to human, livestock and crops and high in safety. Experiment results show that pest control effect of paecilomyces lilacinus can reach 85% to maximum extent.
Description
Technical field
The present invention relates to technical field of pesticide, more particularly, to a kind of Paecilomyces lilacinus oil-suspending agent and preparation method thereof.
Background technology
21 century is the important stage of agricultural development, and the development of agricultural directly affects the overall situation development of national economy.But
In agricultural production process, tend to occur a series of insect pests, make crop production reduction, bring huge economic loss to planting.
Plant root-knot nematode has serious harm, the root of the various vegetables of main harm, killed strain ground to industrial crops
Upper grown is short and small, slow, leaf color abnormal, and result is few, yields poorly, or even causes plant to do sth. in advance death.But for plant root knot
Control of nematode is always using chemical pesticide.Although chemical pesticide has good insecticidal effect, chemical pesticide pollutes environment, right
People and animals are also dangerous, and many chemical agents are all disabled.
Content of the invention
It is an object of the invention to provide a kind of Paecilomyces lilacinus oil-suspending agent, the Paecilomyces lilacinus oil-suspending agent of the present invention
Good stability, Paecilomyces lilacinus is high in oil-suspending agent miospore germination rate, enters moisture and dissipates excellent, disperses in cloud, stable in properties,
In storage process be not in layering and sedimentation problem, heat storage resolution ratio low, low cost, liquid readily permeable to target, drug effect
High.
The invention provides a kind of Paecilomyces lilacinus oil-suspending agent, including the component of following parts by weight：
4～10 parts of medicine activity component, described medicine activity component is Paecilomyces lilacinus；
12～20 parts of auxiliary agent；
70～85 parts of mineral oil.
Preferably, described Paecilomyces lilacinus number selected from biological deposits be CGMCC No.7994 bacterial classification.
Preferably, effective spore amount of described Paecilomyces lilacinus oil-suspending agent is 2.5～4.8 × 109cfu/g.
Preferably, miscellaneous bacteria rate≤0.1% of described Paecilomyces lilacinus oil-suspending agent.
Preferably, suspensibility >=95% of described Paecilomyces lilacinus oil-suspending agent.
Preferably, spore rate >=90% alive of the Paecilomyces lilacinus of described Paecilomyces lilacinus oil-suspending agent.
Preferably, described auxiliary agent includes polyether phosphate class surfactant, Magnesiumaluminumsilicate and aliphatic alcohol polyethenoxy
Ether.
Preferably, on the basis of the parts by weight of auxiliary agent, described auxiliary agent includes 19.5～42.8% polyether phosphate
Class surfactant, 9.7～32.1% Magnesiumaluminumsilicate, 39.0～71.4% AEO.
Present invention also offers the preparation method of the Paecilomyces lilacinus oil-suspending agent described in technique scheme, including following
Step：
A) mineral oil, Paecilomyces lilacinus and auxiliary agent mixed, shear, obtain shearing material；
B) the shearing material obtaining described step a) carries out sand milling, obtains sand milling material；
C) described step b) is obtained the stirring of sand milling material, obtain Paecilomyces lilacinus oil-suspending agent.
The invention provides the invention provides a kind of Paecilomyces lilacinus oil-suspending agent, including the group of following parts by weight
Point：4～10 parts of medicine activity component, described medicine activity component is Paecilomyces lilacinus；12～20 parts of auxiliary agent；Mineral oil 70～85
Part.In the present invention, described Paecilomyces lilacinus oil-suspending agent enter moisture dissipate excellent, in cloud disperse, stable in properties, storage process
In be not in layering and sedimentation problem, heat storage resolution ratio low, low cost；Liquid readily permeable to target, drug effect is high；And this Shen
The Paecilomyces lilacinus oil-suspending agent low toxicity that please provide, insecticidal effect is stable, and to people and animals and crop safety, safe.Experiment
Result shows, the effect of Paecilomyces lilacinus pest control reaches as high as 85%.
Biological deposits explanation
Paecilomyces lilacinus (Paecilomyces lilacinus).This culture presevation manages in Chinese microorganism strain preservation
Committee's common micro-organisms center, address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Chinese Academy of Sciences's microbe research
Institute, deposit number is CGMCC No.7994, and the preservation time is August in 2013 13.
Specific embodiment
The invention provides a kind of Paecilomyces lilacinus oil-suspending agent, including the component of following parts by weight：Pharmaceutically active group
Divide 4～10 parts, described medicine activity component is Paecilomyces lilacinus；12～20 parts of auxiliary agent；70～85 parts of mineral oil.
The Paecilomyces lilacinus oil-suspending agent that the present invention provides preferably includes 4～10 parts of Paecilomyces lilacinus, more preferably 5～
8 parts, most preferably 6 parts.
In the present invention, described Paecilomyces lilacinus number selected from biological deposits be CGMCC No.7994 bacterial classification.
In the present invention, the preparation method of described Paecilomyces lilacinus preferably includes：Paecilomyces lilacinus is inoculated in liquid training
In foster base, fermented under the conditions of logical oxygen, obtained Paecilomyces lilacinus zymotic fluid.
In the present invention, the inoculum concentration of described Paecilomyces lilacinus is preferably 2～8%, more preferably 5%.
In the present invention, the fluid nutrient medium that described Paecilomyces lilacinus uses preferably includes the component of following weight portion：Sugarcane
1～50 part of sugar, 1～10 part of ammonium nitrate, 0.5～5 part of disodium hydrogen phosphate, 0.1～1.0 part of magnesium sulfate, ferric sulfate 0.01～1.0
Part, 0.1～10 part of potassium chloride.
In the present invention, the fluid nutrient medium that described Paecilomyces lilacinus uses preferably includes 1～50 portion of sucrose, more preferably
20～35 parts, most preferably 25 parts；The fluid nutrient medium that described Paecilomyces lilacinus uses preferably includes 1～10 part of ammonium nitrate, more
It is preferably 4～8 parts, most preferably 6 parts；The fluid nutrient medium that described Paecilomyces lilacinus uses preferably includes 0.5～5 part of phosphoric acid hydrogen
Disodium, more preferably 2～3.5 parts, most preferably 2.5 parts；The fluid nutrient medium that described Paecilomyces lilacinus uses preferably includes 0.1
～1.0 parts of magnesium sulfate, more preferably 0.3～0.9 part, most preferably 0.8 part；The fluid nutrient medium that described Paecilomyces lilacinus uses
Preferably include 0.01～1.0 part of ferric sulfate, more preferably 0.05～0.4 part, most preferably 0.35 part；Described Paecilomyces lilacinus
3.5 part.
In the present invention, the fermentation of described Paecilomyces lilacinus is preferably carried out under agitation, and the condition of stirring is preferably
50～500rpm, more preferably 100～300rpm；The fermentation time of described Paecilomyces lilacinus is preferably 49～96h, more preferably
60h；Fermentation temperature is preferably 27～28 DEG C.
In the present invention, described logical oxygen be specially fermentation when be passed through sterile air, described oxygen-supply speed be preferably 4～
8L/min, more preferably 6.5L/min.
In the present invention, pressure during described Paecilomyces lilacinus liquid fermentation is 0.1 ± 0.02MPa.
In the present invention, described Paecilomyces lilacinus preferably carries out illumination during the fermentation, and described illumination condition is：Power
For 15W～100W, the more preferably incandescent lamp of 40W.
The present invention is 2～15 × 10 preferably in Paecilomyces lilacinus fermentation total viable count10During cfu/g, stop fermentation.
After completing the fermentation of described Paecilomyces lilacinus, preferred pair Paecilomyces lilacinus zymotic fluid of the present invention carries out separation of solid and liquid,
Obtain Paecilomyces lilacinus thalline；Paecilomyces lilacinus thalline is dried, pulverized and sieved, is obtained the pale purple plan of solid, powdery blue or green
Mould.The method that described separation of solid and liquid preferably employs centrifugation, centrifugal rotational speed is preferably 1500～3000rpm, more preferably
2000rpm；Described centrifugation time is preferably 15～30min, more preferably 20min.In the present invention, the temperature of described drying is excellent
Elect 30～50 DEG C as, more preferably 35～45 DEG C, most preferably 40 DEG C；The time of described drying is preferably 20～45h, more preferably
For 30～40h, most preferably 35h；Paecilomyces lilacinus thalline is dried to moisture preferably between 25～35% by the present invention,
More preferably 27～32%, most preferably 30%.
The method that the present invention pulverizes to described solid fermentation culture medium does not have special restriction, using those skilled in the art
Known to breaking method.In the present invention, described solid, powdery Paecilomyces lilacinus sieves and preferably employs 80～100 mesh, more
It is preferably 85～95 mesh, most preferably 90 mesh.
In the present invention, effective spore amount of described Paecilomyces lilacinus oil-suspending agent is 2.5～4.8 × 109Cfu/g,
More preferably 3.5 × 109cfu/g.Miscellaneous bacteria rate≤0.1% of described Paecilomyces lilacinus oil-suspending agent.Described Paecilomyces lilacinus
Suspensibility >=95% of oil-suspending agent, more preferably >=98%.The work of the Paecilomyces lilacinus of described Paecilomyces lilacinus oil-suspending agent
Spore rate >=90%.
In the present invention, described Paecilomyces lilacinus oil-suspending agent preferably includes 12～20 parts of auxiliary agent, more preferably 15～
18 parts, most preferably 16 parts.In the present invention, described auxiliary agent includes polyether phosphate class surfactant, Magnesiumaluminumsilicate and fat
Fat alcohol APEO.The present invention is to described polyether phosphate class surfactant, Magnesiumaluminumsilicate and AEO
Source there is no special restriction, using conventional polyether phosphate class surfactant, Magnesiumaluminumsilicate and fatty alcohol polyoxy second
The commercially available prod of alkene ether.
On the basis of the parts by weight of auxiliary agent, auxiliary agent of the present invention preferably includes 19.5～42.8% polyether phosphate
Class surfactant, more preferably 22～35%；Described auxiliary agent preferably includes 9.7～32.1% Magnesiumaluminumsilicate, more preferably
15～28%；Described auxiliary agent preferably includes 39.0～71.4% AEO, and more preferably 45～58%.
The Paecilomyces lilacinus oil-suspending agent that the present invention provides preferably includes 70～85 parts of mineral oil, and more preferably 75～80
Part, most preferably 78 parts.The present invention is not particularly limited to the species of described mineral oil and source, using those skilled in the art
Known to pesticide oil suspension float agent mineral oil commercial goods.Specific mineral oil is preferably methyl oleate.The present invention
Described mineral oil has good medicine stability, also can improve the drug effect of medicine.
Present invention also offers the preparation method of the Paecilomyces lilacinus oil-suspending agent described in technique scheme, including following
Step：
A) mineral oil, Paecilomyces lilacinus and auxiliary agent mixed, shear, obtain shearing material；
B) the shearing material obtaining described step a) carries out sand milling, obtains sand milling material；
C) described step b) is obtained the stirring of sand milling material, obtain Paecilomyces lilacinus oil-suspending agent.
In the present invention, the mixing of described mineral oil, Paecilomyces lilacinus and auxiliary agent, shearing are preferably mineral oil average mark
Become 2 parts, the 1st part of mineral oil carried out the first shearing, then mix with Paecilomyces lilacinus, second shears, then mix with auxiliary agent, the
Three shearing, then with the 2nd part of mineral oil, four times shearing, after having added all raw materials, be further continued for shearing 20～40min, more excellent
Elect 25～35min, most preferably 30min as.The present invention does not have special restriction to the equipment of mixing, shearing, using this area
Mixing known to technical staff, shearing equipment, such as cutting kettle.The rotating speed of described shearing be preferably 5000rpm～
20000rpm, more preferably 10000rpm～15000rpm.
After obtaining shearing material, the present invention, by described shearing material sand milling, obtains sand milling material.In the present invention, described sand milling
Granularity is preferably 2～8 μm, more preferably 3～7 μm, most preferably 4～6 μm.The present invention is not special to the equipment of described sand milling
Limit, using sand milling device well known to those skilled in the art, such as sand mill.
After obtaining sand milling material, described sand milling material is stirred by the present invention, obtains Paecilomyces lilacinus oil-suspending agent.In the present invention
In, the mixing time of described sand milling material is preferably 10～30min, more preferably 15～25min, most preferably 20min.The present invention
There is no special restriction to the equipment of described stirring, using mixing plant well known to those skilled in the art, such as mixer.
The Paecilomyces lilacinus oil-suspending agent present invention being provided with reference to specific embodiment and preparation method thereof does into one
Walk detailed introduction, technical scheme includes but is not limited to following examples.
Embodiment 1
The preparation of Paecilomyces lilacinus
It is seeded in after Paecilomyces lilacinus Amplification Culture in Liquid Culture, described fluid nutrient medium composition and its weight ratio are
30 parts of sucrose, 1.5 parts of ammonium nitrate, 2 parts of disodium hydrogen phosphate, 0.25 part of magnesium sulfate, 0.05 part of ferric sulfate, 0.5 part of potassium chloride.Fermentation
Condition is：Temperature is 27 DEG C, no stirring, logical oxygen, pressure inside the tank 0.1MPa, time 72 hours, illumination：36V 40W incandescent lamp, when
Total viable count reaches 5 × 1010During cfu/g, stop fermentation and obtain Paecilomyces lilacinus zymotic fluid.Paecilomyces lilacinus zymotic fluid is carried out solid
Liquid separates, and obtains Paecilomyces lilacinus thalline, Paecilomyces lilacinus thalline is dried, pulverized and sieved：Dry under the conditions of 40 DEG C
Dry 35h, dry to Paecilomyces lilacinus thalline moisture be 30%, pulverized 100 mesh sieves, obtained Paecilomyces lilacinus.
Embodiment 2
The preparation of Paecilomyces lilacinus oil-suspending agent：In terms of parts by weight, 80 parts of methyl oleate is divided into 2 parts, will
1st part of methyl oleate is sheared, then mixes, shears with the Paecilomyces lilacinus of 6 parts of embodiments 1 preparation, then with 3 parts of alumina silicate
Magnesium mixing, shearing, then mix, shear with 8 parts of AEOs, then mix with 5 parts of polyether phosphate class surfactants
Close, shear, then mix, shear with the 2nd part of methyl oleate, treat that all raw materials all mix, sheared, be further continued for shearing 30min,
Obtain shearing material, the rotating speed of above-mentioned shearing is 15000rpm；Shearing material is carried out sand milling, obtains 5 μm of sand milling material, by sand milling
After material stirring 20min, obtain Paecilomyces lilacinus oil-suspending agent.The quality control index of the Paecilomyces lilacinus oil-suspending agent obtaining is such as
Shown in table 1.
The quality control index of the Paecilomyces lilacinus oil-suspending agent of table 1 embodiment 2 preparation
Embodiment 3
The preparation of Paecilomyces lilacinus oil-suspending agent：In terms of parts by weight, 70 parts of methyl oleate is divided into 2 parts, will
1st part of methyl oleate is sheared, then mixes, shears with the Paecilomyces lilacinus of 8 parts of embodiments 1 preparation, then with 2 parts of alumina silicate
Magnesium mixing, shearing, then mix, shear with 9 parts of AEOs, then mix with 4 parts of polyether phosphate class surfactants
Close, shear, then mix, shear with the 2nd part of methyl oleate, treat that all raw materials all mix, sheared, be further continued for shearing 35min,
Obtain shearing material, the rotating speed of above-mentioned shearing is 12000rpm；Shearing material is carried out sand milling, obtains 8 μm of sand milling material, by sand milling
After material stirring 30min, obtain Paecilomyces lilacinus oil-suspending agent.
Embodiment 4
The preparation of Paecilomyces lilacinus oil-suspending agent：In terms of parts by weight, 84 parts of methyl oleate is divided into 2 parts, will
1st part of methyl oleate is sheared, then mixes, shears with the Paecilomyces lilacinus of 10 parts of embodiments 1 preparation, then with 4 parts of silicic acid
Magnalium mixing, shearing, then mix, shear with 10 parts of AEOs, then with 6 parts of polyether phosphate class surfactants
Mixing, shearing, then mix, shear with the 2nd part of methyl oleate, treat that all raw materials all mix, sheared, be further continued for shearing
40min, obtains shearing material, and the rotating speed of above-mentioned shearing is 10000rpm；Shearing material is carried out sand milling, obtains 6 μm of sand milling material,
Sand milling material is stirred after 25min, obtains Paecilomyces lilacinus oil-suspending agent.
Embodiment 5
The test of pesticide effectiveness：This test is carried out in Jiangxi Province, and preventing and treating kind is dragon fruit, and management is good, and root-knot nematode occurs
Medium weighting.Experimental field is divided into four regions by this test, and region 1 is control group, only along ridge spray clear water；Region 2 is suitable
Ridge sprays 1000 times of dilutions of Paecilomyces lilacinus of preparation in embodiment 3；Region 3 sprays Paecilomyces lilacinus wettable powder along ridge
1000 times of dilutions, the concentration of medicine activity component is identical with region 2；Region 4 sprays Paecilomyces lilacinus aqueous suspension agent along ridge
1000 times of dilutions, the concentration of medicine activity component is identical with region 2 and 3；The dosage in four regions is identical, 1 day after dispenser,
3 days, 5 days, each investigation in 7 days once borer population alive, calculate Revision insect recluced rate.As shown in table 2, the data by table 2 is permissible for concrete outcome
Draw, the Revision insect recluced rate highest in region 2, show the insecticidal effect of Paecilomyces lilacinus oil-suspending agent of present invention preparation preferably,
Its drug effect is above wettable powder and the aqueous suspension agent of same concentration.
Desinsection (root-knot nematode) effect of table 2 Paecilomyces lilacinus oil-suspending agent
As seen from the above embodiment, the Paecilomyces lilacinus oil-suspending agent stability of present invention preparation is high, and viable count is high, to line
The control rate of worm may be up to 82.7%, and its drug effect is above wettable powder and the aqueous suspension agent of same concentration.
Embodiment 6
The test of pesticide effectiveness：This test is carried out in Jiangxi Province, and preventing and treating kind is peanut, and management is good, occurs root-knot nematode tight
Weight.Experimental field is divided into four regions by this test, and region 1 is control group, a spray clear water；Region 2 sprays in embodiment 2
2000 times of dilutions of Paecilomyces lilacinus oil-suspending agent of preparation；Region 3 sprays the 1500 times of dilutions of Paecilomyces lilacinus wettable powder
Liquid, the concentration of medicine activity component is identical with region 2；Region 4 sprays 2000 times of dilutions of Paecilomyces lilacinus aqueous suspension agent, medicine
The concentration of active component is identical with region 2 and 3；The dosage in four regions is identical, and 1 day after dispenser, 3 days, each investigation in 7 days was once
Root nodule situation.Result shows, the insecticidal effect of the Paecilomyces lilacinus oil-suspending agent of present invention preparation is best, the control rate to nematode
May be up to 85%, its drug effect is above wettable powder and the aqueous suspension agent of same concentration.
The above is only the preferred embodiment of the present invention it is noted that ordinary skill people for the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (9)
1. a kind of Paecilomyces lilacinus oil-suspending agent is it is characterised in that include the component of following parts by weight：
4～10 parts of medicine activity component, described medicine activity component is Paecilomyces lilacinus；
12～20 parts of auxiliary agent；
70～85 parts of mineral oil.
2. Paecilomyces lilacinus oil-suspending agent according to claim 1 is it is characterised in that described Paecilomyces lilacinus is selected from life
Thing deposit number is the bacterial classification of CGMCC No.7994.
3. Paecilomyces lilacinus oil-suspending agent according to claim 1 is it is characterised in that described Paecilomyces lilacinus oil suspends
Effective spore amount of agent is 2.5～4.8 × 109cfu/g.
4. the Paecilomyces lilacinus oil-suspending agent according to claims 1 to 3 any one is it is characterised in that described is pale purple
Miscellaneous bacteria rate≤0.1% of Paecilomyces varioti oil-suspending agent.
5. Paecilomyces lilacinus oil-suspending agent according to claim 1 is it is characterised in that described Paecilomyces lilacinus oil-suspending agent
Suspensibility >=95%.
6. according to claim 1 or 5 Paecilomyces lilacinus oil-suspending agent it is characterised in that described Paecilomyces lilacinus oil is outstanding
Spore rate >=90% alive of the Paecilomyces lilacinus of floating agent.
7. Paecilomyces lilacinus oil-suspending agent according to claim 1 is it is characterised in that described auxiliary agent includes polyether phosphate
Class surfactant, Magnesiumaluminumsilicate and AEO.
8. the Paecilomyces lilacinus oil-suspending agent according to claim 1 or 7 is it is characterised in that with the parts by weight of auxiliary agent be
Benchmark, described auxiliary agent include 19.5～42.8% polyether phosphate class surfactant, 9.7～32.1% Magnesiumaluminumsilicate,
39.0～71.4% AEO.
9. the preparation method of the Paecilomyces lilacinus oil-suspending agent described in claim 1～8 any one, comprises the following steps：
A) mineral oil, Paecilomyces lilacinus and auxiliary agent mixed, shear, obtain shearing material；
B) the shearing material obtaining described step a) carries out sand milling, obtains sand milling material；
C) described step b) is obtained the stirring of sand milling material, obtain Paecilomyces lilacinus oil-suspending agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610841921.XA CN106417387A (en) | 2016-09-23 | 2016-09-23 | Paecilomyces lilacinus oil suspension agent and preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610841921.XA CN106417387A (en) | 2016-09-23 | 2016-09-23 | Paecilomyces lilacinus oil suspension agent and preparation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106417387A true CN106417387A (en) | 2017-02-22 |
Family
ID=58166705
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610841921.XA Pending CN106417387A (en) | 2016-09-23 | 2016-09-23 | Paecilomyces lilacinus oil suspension agent and preparation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106417387A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1433692A (en) * | 2003-03-05 | 2003-08-06 | 湖北省林业科学研究院 | Bacillus thuringiensis oil suspension concentrate pesticide |
| CN101273729A (en) * | 2008-05-20 | 2008-10-01 | 重庆大学 | Fungal spore oil suspending agent |
| CN101611707A (en) * | 2009-08-12 | 2009-12-30 | 海南利蒙特生物农药有限公司 | A kind of bacillus subtilis oil suspending agent and preparation method thereof |
| CN102742607A (en) * | 2012-07-23 | 2012-10-24 | 中国农业大学 | Paecilomyces lilacinus fungal spore oil suspending agent and preparation method as well as application thereof |
| CN103773697A (en) * | 2013-12-05 | 2014-05-07 | 江西天人生态股份有限公司 | paecilomyces lilacinus and application thereof |
| CN105794855A (en) * | 2014-12-30 | 2016-07-27 | 姜汉军 | Control method of potato cyst nematode |
-
2016
- 2016-09-23 CN CN201610841921.XA patent/CN106417387A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1433692A (en) * | 2003-03-05 | 2003-08-06 | 湖北省林业科学研究院 | Bacillus thuringiensis oil suspension concentrate pesticide |
| CN101273729A (en) * | 2008-05-20 | 2008-10-01 | 重庆大学 | Fungal spore oil suspending agent |
| CN101611707A (en) * | 2009-08-12 | 2009-12-30 | 海南利蒙特生物农药有限公司 | A kind of bacillus subtilis oil suspending agent and preparation method thereof |
| CN102742607A (en) * | 2012-07-23 | 2012-10-24 | 中国农业大学 | Paecilomyces lilacinus fungal spore oil suspending agent and preparation method as well as application thereof |
| CN103773697A (en) * | 2013-12-05 | 2014-05-07 | 江西天人生态股份有限公司 | paecilomyces lilacinus and application thereof |
| CN105794855A (en) * | 2014-12-30 | 2016-07-27 | 姜汉军 | Control method of potato cyst nematode |
Non-Patent Citations (4)
| Title |
|---|
| 梁利宝 编: "《微生物肥料与农药在现代农业生产中的应用》", 31 July 2012, 中国农业科学技术出版社 * |
| 潘沧桑: "淡紫拟青霉菌剂的研究开发", 《精细与专用化学品》 * |
| 王中康 等主编: "《现代生物农药100问》", 31 October 2014, 中国农业科学技术出版社 * |
| 郭志斌: "淡紫拟青霉液体发酵工艺及其制剂的研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101273729A (en) | Fungal spore oil suspending agent | |
| CN108064891A (en) | The pesticide control of Citrus Huanglongbing pathogen | |
| CN111903713A (en) | Tank-mixing auxiliary agent and preparation method thereof | |
| CN105532735A (en) | Bactericidal and insecticidal composition containing Bacillus amyloliquefaciens and matrine | |
| WO2014193162A1 (en) | Preparation method for biopesticide using paecilomyces sp. strain | |
| CN103329914B (en) | Acaricidal composition containing pyriminostrobin and bifenazate | |
| CN104381294B (en) | A kind of insecticide acaricide composition containing sulfur-phosphor and Envidor | |
| CN102626092B (en) | Azadirachtin aqueous solution for treating root-knot nematode disease and preparation method thereof | |
| KR101983021B1 (en) | The composition of fungicidal and insecticidal nano-emulsion | |
| CN101218922A (en) | High-efficiency and low-toxin seed-treating agent | |
| CN106417387A (en) | Paecilomyces lilacinus oil suspension agent and preparation method | |
| CN105613511B (en) | A kind of agricultural pesticide composition and application thereof | |
| CN105145635B (en) | A kind of composition for helicopter dispenser prevention fall webworms and preparation method thereof | |
| CN108812707A (en) | One kind killing thrips plant essential oil composition, preparation and its application | |
| CN103314986B (en) | Cycloxaprid and spirotetramat-containing insecticidal composition | |
| CN106359455A (en) | Paecilomyces lilacinus wettable powder and preparation method thereof | |
| CN103548855B (en) | Zhongshengmycin-containing bactericidal composition and application thereof | |
| CN102224831B (en) | Control method of tetranychus cinnabarinus based on tomato extracts | |
| CN101731261B (en) | Pesticide preparation for preventing and treating quadrastichus erythrinae kim | |
| CN105010383A (en) | Insecticidal composition containing dinotefuran and indoxacarb and preparation method thereof | |
| CN103444725A (en) | Mite-killing composition containing pyriminostrobin and clofentezine | |
| CN106489927A (en) | A kind of paecilomyces fumosoroseus oil-suspending agent and preparation method thereof | |
| CN103548832A (en) | Acaricidal composition containing pyriminostrobin and cyflumetofen | |
| CN107935748A (en) | Insecticide fertilizer for unmanned plane sprinkling and preparation method thereof | |
| CN101755829B (en) | Pesticide preparation for preventing pest erythrina gall wasp |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170222 |
|
| RJ01 | Rejection of invention patent application after publication |