CN101273729A - Fungal spore oil suspending agent - Google Patents
Fungal spore oil suspending agent Download PDFInfo
- Publication number
- CN101273729A CN101273729A CNA2008100697049A CN200810069704A CN101273729A CN 101273729 A CN101273729 A CN 101273729A CN A2008100697049 A CNA2008100697049 A CN A2008100697049A CN 200810069704 A CN200810069704 A CN 200810069704A CN 101273729 A CN101273729 A CN 101273729A
- Authority
- CN
- China
- Prior art keywords
- oil
- suspending agent
- fungal spore
- spore
- dilute
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides an oil suspending agent of fungal spore, consisting of fungal spore powder, surfactant and suspension oil, wherein, according to parts by weight, the fungal spore powder accounts for 1 to 50 percent, the surfactant accounts for 1 to 25 percent and the suspension oil accounts for 25 to 98 percent. The product of the invention has the advantages of low application cost, convenient use, stable prevention effects and long storage time, etc.
Description
Technical field
The present invention relates to a kind of fungal farm chemicals preparation, particularly a kind of fungal spore oil suspending agent.
Background technology
The long-term a large amount of use of chemical pesticide, heavy damage the ecological balance, pollute natural environment.Adopt one of important means free from environmental pollution, as the nontoxic biopesticide instead of chemical agricultural chemicals of people and animals to be become disease, worm, grass comprehensive control.Fungi is most important microbe groups, is used for the history of the existing last 100 years of biological control.Fungi mainly relies on has biological living, as mycelium and spore, carries out biological control; Its control efficiency is subjected to multiple natural environment, and the influence as factors such as temperature, humidity, daylight causes the preventive effect instability; Improve the important content that the fungus biocontrol effect becomes fungi preparation research by formulation.Same fungal organism live body is subjected to the influence of number of chemical material, cause the research of the fungal organism formulations of pesticide to lag behind, kind is single, existing preparation often can not satisfy length storage period simultaneously, biologically active is stable, field efficacy is stable, easy to use and prevent and treat commercialization conditions such as cost is low, and this has seriously restricted developing rapidly of fungal organism preparation.
At present, the formulation that fungal farm chemicals is commonly used has pulvis, wetting powder, granule, oil-suspending agent etc.Pulvis is the formulation that fungi is developed the earliest and uses, and it is that hypha,hyphae or spore and additives mixed such as filler (as diatomite, talcum powder, precipitated calcium carbonate, kaolin) and ultra-violet radiation resisting agent are processed.Pulvis effectively overcomes the not shortcoming of anti-storage of fungi, but after pulvis directly dusts, and spore or hyphal body rely on and absorb moisture and sprout or regrow from environment.Fungus spore germination or regrow and require high humidity is subjected to humidity changes in the environment influence greatly, the less stable that use in the field.Therefore, the fungi pulvis exists use the season that is difficult in dry area and drying.In addition, pulvis easily flies away when using, and produces a large amount of dust, contaminated environment.
Granule after the pulvis granulated processed produce in the time of avoiding pulvis to use a large amount of dust and further the activity of protection hypha,hyphae or spore receive researchers' concern; behind first green muscardine fungus hyphostoma particle agent BIO1020 that the eighties, Bayer A.G developed, the fungal farm chemicals of nearly 10 kinds of granule formulations is registered in succession.Domestic Xu Qing is rich, Zhang Aiwen has done more research to fungal granular formulation, obtains certain effect.Knudsen et al. (1990) is optimized granule technology, utilizes sodium alginate parcel fungal mycelium to make capsule, stores 1 year mycelia active for 20 ℃ and still has 70%.Jung and Mugnier (1989) then utilizes long hornbeam xanthans parcel liquid to give birth to that the activity of normal temperature after 1 year is 100% after the spore drying.Liu Zhiqiang (1994) has been done Primary Study to the polymeric gel white muscardine fungi, obtains good result.Because hypha,hyphae or spore in granule and the capsule are difficult to diffuse out rapidly, control efficiency is slow, and only limitation is prevented and treated minority soil insect (as grub), bored moth property (as corn borer) insect and sanitary insect pest cockroach etc.
Wettable preparation (comprising wetting powder and water-dispersible granules) has easy to use, and prevention and treatment range is wide, and advantage environmentally safe and that can promote hydrophobic fungal spore to disperse rapidly in water was once becoming the focus of fungi preparation research.China Zhang Aiwen (1992) has done than systematic research the white muscardine fungi wetting powder.The fungi wetting powder of registering accounts for about 40% of registration fungal farm chemicals at present.But need to add some ionic surfactants (as drawing back powder etc.) in the wet preparation, ionic surfactant and fungal spore Long contact time often influence the fungal spore vigor.The same with pulvis, wetting powder, water-dispersible granules require high to ambient humidity, the field efficacy shakiness.Nearly more than ten years chemical pesticide dosage form research and the achievement that obtains of exploitation, wherein water base property novel form is because high safety, environmental protection and the cheap main developing direction of chemical pesticide formulation that becomes, the water base property of fungi formulation, comprise that researchs such as microcapsule formulations, emulsion carry out at home, but water base property novel form is unfavorable for that based on water fungi stores.
At the end of the eighties in last century, the appearance of fungal oil suspending agent formulation makes fungal farm chemicals research obtain breakthrough with application.People such as Prior (1988) are by replacing aqueous suspension can enlarge conidial environmental adaptation scope to discovering with the conidium oil-suspending agent of white muscardine fungi [Beauvaria bassiana (Bals.)], and effectively solving fungal farm chemicals needs a super-humid conditions and the short difficult problem of product period of storage.People such as Bateman studies confirm that further the muscardine spore powder is dispersed in the oil, under the 35%RH condition, LD50 to desert locust [(Schistocerca gregaria (Forskal)] in the time of 5 days is 8900 spores/head, and much smaller than the 106 spores/head of aqueous suspensions, virulence improves 112 times.Nearly more than 10 years fungal spore oil suspending agents have developed into a kind of novel form of fungus insecticide, the wherein multiple locust fungal oil suspending agent that kills is registered in succession in states such as Africa, Australia and China, and in the arid area by the various locusts of large area control, become the major microorganisms insecticide of controlling locust in the world.
Though oil-suspending agent has successfully overcome the defective that fungal spore is difficult to use, insecticidal effect is subjected to humidity effect in the environment in dry environment, but there is the problem of the following aspects in oil-suspending agent: the cost of (1) formulation oils is higher, need be with spraying medicine after oil or the special-purpose diluted during field rotating medicine; (2) oil-suspending agent can only adopt the ultra low volume engine-driven atomiser to carry out spray pesticide, make various fungal oil suspending agents mainly concentrate the insect of taking place by control after government's centralized purchasing, and less to disperseing that the insect application takes place, be not suitable for the agricultural production mode that China single household small size is prevented and treated; These have restricted the fungi use greatly.Therefore, low, easy to use, the affected by environment little fungi oil base novel form of development cost becomes the focus of fungi preparation research.It is a kind of fungi novel form-aqueous emulsion of basic research that Batta (2004) attempts with the oil-suspending agent, on the basis of oil-suspending agent, add the aqueous emulsion that entry and emulsifier are processed into moisture 45% W/O (Water-In-Oil), adopt two spotted spider mite (Tetranychus cinnabarinus Boisd.) and Bemisia tabaci (Bemisia tabaci Genn.) on the conventional spraying control tobacco, and the moisture content in the discovery oil-suspending agent can increase the oil-suspending agent control efficiency, but, preserve 10 all backs spore germination rates and be reduced to below 80% owing to contain water in the preparation.In addition, oil-suspending agent only adapts to and hydrophobic fungal spore, and hydrophilic fungal spore can not be mixed with oil-suspending agent, and this also limits the application of fungal oil suspending agent.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, the advantage separately in conjunction with existing fungal oil suspending agent and aqueous emulsion provides a kind of cost fungal spore oil suspending agent low, easy to use of using.
The object of the present invention is achieved like this: a kind of fungal spore oil suspending agent, it is made up of fungal spore powder, surfactant and suspended oil, and wherein the fungal spore powder accounts for 1～50%, surfactant comprise 1～25%, suspended oil accounts for 25～98%, in weight portion.
The composition proportioning of above-mentioned fungal spore oil suspending agent is preferably the fungal spore powder in weight portion and accounts for 15～35%, surfactant comprise 5～15%, and suspended oil accounts for 40～80%.
Above-mentioned fungal spore is that the hydrophily fungal spore is or/and the hydrophobicity fungal spore; Surfactant is a nonionic surface active agent; Suspended oil is a vegetable oil.
Above-mentioned fungi is green muscardine fungus, white muscardine fungi, Verticillium lecanii, Trichoderma, colletotrichum gloeosporioides Penz or their Mixed Microbes, and above-mentioned nonionic surface active agent is fatty alcohol-polyoxyethylene ether, alkylphenol polyoxyethylene, class of department, tween or their mixture; Above-mentioned vegetable oil is soybean oil, rapeseed oil, cotton seed oil, palm oil, sesame oil, corn oil or their mixtures or other analogs.
Fungal spore oil suspending agent of the present invention is the good surfactant of compatibility that adds aforementioned proportion on the basis of existing fungal oil suspending agent, add the state that water can form oil-in-water, oil parcel spore during use, sufficient moisture can promote the sprouting of spore, the spore of oil parcel contacts with host's epidermis after the water evaporates, has the characteristic of oil-suspending agent.So fungal spore oil suspending agent has oil-suspending agent little characteristics affected by environment, a large amount of moisture further reduces the dependence of preparation to environment on every side simultaneously.
Above-mentioned fungal spore oil suspending agent is easy to use, and dilute with water during use can adopt multiple insecticide-applying way as required; Specific practice is: adopt the spraying of ultra low volume technology behind 1～10 times of the dilute with water; Or adopt the spraying of low capacity technology behind 10～500 times of the dilute with waters; Or adopt conventional spray behind 500～3000 times of the dilute with waters.The dilute with water multiple is preferably 3～7 times when adopting the spraying of ultra low volume technology, and the dilute with water multiple is preferably 100～300 times when adopting the spraying of low capacity technology, and the dilute with water multiple is preferably 1000～2000 times during the routine techniques spraying.
Beneficial effect of the present invention is:
1, product of the present invention is lower than the use cost of existing fungal oil suspending agent.The surfactant that contains high concentration in the fungal oil suspending agent of the present invention can form stable emulsion after adding water, and direct dilute with water when using in the field does not need oil or the special-purpose diluted of using price high.Use 10ml 20% green muscardine fungus oil-suspending agent by every mu, can save about 0.4～0.6 yuan of the plant flux oil of 40ml, reduce cost 20～30%.
2, product of the present invention is more easy to use than existing fungal oil suspending agent.Use and to determine insecticide-applying way according to the field drug-spraying instrument in the field, can select ultra low volume spraying, low-gallonage spraying or conventional spray; Determine to add what of water according to insecticide-applying way and environmental condition.This has overcome the limitation that existing fungal oil suspending agent can only adopt ultra low volume spraying, has enlarged the fungal oil suspending agent scope of application.
3, fungal spore oil suspending agent preventive effect of the present invention is stable, and is affected by environment little, can obviously improve the preventive effect of traditional fungal spore aqueous suspension agent.Owing to contain the surfactant of high concentration in the preparation, form aqueous emulsion behind the thin up of field, and sporocyst is wrapped in the oil droplet, has oil-suspending agent little characteristics affected by environment, a large amount of moisture can promote the sprouting of spore simultaneously, thereby reduces the dependence of preparation to ambient humidity.
4, be adapted to the fungi of dissimilar spores.Existing fungal oil suspending agent only is suitable for hydrophobic fungal spore, contains surfactant in the fungal spore oil suspending agent of the present invention, except being suitable for hydrophobic fungal spore, also is suitable for hydrophilic fungal spore.
5, the surfactant selected for use of the present invention is a nonionic, and is good with the fungal spore compatibility in oil-suspending agent, and the not obviously influence of preparation stored phase to oil-suspending agent has kept oil-suspending agent long characteristics storage period.
Embodiment
Mode below by embodiment further specifies the present invention, does not therefore limit the present invention among the described scope of embodiments.
Embodiment 1
For of the influence of proof list surface-active agent to the fungal spore bin stability among the present invention, the inventor press table 1 with rapeseed oil respectively with different fungi xerospore powder and surfactant stirrer for mixing 5 minutes (rotating speed 1800rpm) at 1 liter, respectively be mixed with the different fungal spore oil suspending agents of 400 grams, the grournd glass normal temperature laboratory of the 500ml that packs into sealing is stored.Every month, a little diameter that is seeded in the 1/4SDA flat board was on 9 millimeters glassine paper disks with the oese picking behind the mixing respectively with oil-suspending agent, evenly coating, 26 ± 1 ℃ of constant temperature culture, got glassine paper disk cotton blue dyeing back microscopy in 24 hours, count 200～300 spores, the spore count that record is sprouted, calculate germination rate, green muscardine fungus, white muscardine fungi, Trichoderma is handled contrast not add surfactant, because Verticillium lecanii, the colletotrichum gloeosporioides Penz spore is hydrophilic spore, can not hold with oil phase under the effect that does not have emulsifier, so adopt xerospore powder low temperature (4～6 ℃) sealing to be stored as contrast, each handles three repetitions.
As can be seen from Table 1: the storage of fungal spore in the oil-suspending agent of concentrated surfactant is after 6 months and 12 months, its stability and contrast no significant difference, the surfactant of the high concentration in the illustrative experiment to the obviously influence of the active nothing of fungal spore, does not influence the fungal spore bin stability in oil-suspending agent.
Table 1 surfactant is to the influence of fungal spore oil suspending agent bin stability
Embodiment 2
The green muscardine fungus oil-suspending agent disinfestation determination of activity of different surfaces activating agent.
Green muscardine fungus, white muscardine fungi and Verticillium lecanii are insect pathogenic fungus, directly infect host insect by body wall.We are test strain to kill the locust green muscardine fungus, and the research oil-suspending agent is to the influence of insecticidal activity.According to heterogeneity percentage by weight in the table 2 with soybean salad oil, green muscardine fungus xerospore powder (50,000,000,000 spore/gram) and different surfaces activating agent stirrer for mixing 5 minutes (rotating speed 1800rpm) at 1 liter, preparation 400 grams 20% muscardine spore oil-suspending agent, the grournd glass normal temperature laboratory of the 500ml that packs into sealing is stored.From the first generation locust population of indoor feeding, select locust polypide heavy phase with healthy adult, put into that kind the diameter of wheat seeding to be arranged be 35cm * 50cm cylinder mould, 50 of every processing.Consumption employing POTTER spray tower (BURKARD company) ultra low volume spraying of press the 50ul/ cage behind 4 times of the different green muscardine fungus oil-suspending agent dilute with waters.If the consumption ultra low volume spraying by the 50ul/ cage after the green muscardine fungus oil-suspending agent dilutes 4 times with oil is contrast with not dispenser.Repeat 3 times.Adjusting insectary room temperature to 28～33 ℃, relative moisture 30～60% is changed fresh wheat seeding after three days.Regularly observe every day, and each dead borer population of handling of record calculates LT90 with DPS software.
Table 2 variable concentrations and type list surface-active agent influence green muscardine fungus oil-suspending agent virulence
| Preparation | Surfactant | LT90(d) |
| ① | Contain 12% fatty alcohol-polyoxyethylene ether | 9.4±0.7a |
| ② | Contain 15% alkylphenol polyoxyethylene | 8.7±1.5a |
| ③ | Contain 3% fatty alcohol-polyoxyethylene ether+4% alkylphenol polyoxyethylene | 8.5±1.1a |
| ④ | Contain 2% fatty alcohol-polyoxyethylene ether+3% alkylphenol polyoxyethylene+1% tween | 8.7±1.3a |
| ⑤ | Contain 1% fatty alcohol-polyoxyethylene ether+3% alkylphenol polyoxyethylene+1% class of department | 8.9±0.9a |
| CK | Do not contain surfactant | 8.9±1.7a |
As can be seen from Table 2: 1. above-mentioned～5. different 20% (W/W) green muscardine fungus oil-suspending agents with the LT90 of 5 times of water dilution back ultra low volume sprayings control Asiatic migratory locusts with contrast the green muscardine fungus oil-suspending agent level on do not have significant difference (p=0.05), illustrate that green muscardine fungus oil-suspending agent insecticidal effect under the environmental condition of high temperature, low humidity is stable, preventive effect is consistent with the green muscardine fungus oil-suspending agent.Use 10ml 20% green muscardine fungus oil-suspending agent by every mu, can save the plant flux oil (about 0.4～0.6 yuan) of 40ml.
Embodiment 3
Green muscardine fungus oil-suspending agent different administration method influences the Asiatic migratory locust virulence.
150 gram soybean oils, 50 gram palm oils, 28 gram fatty alcohol-polyoxyethylene ether, 32 gram alkylphenol polyoxyethylene and 120 grams are contained pack into 1 liter stirrer for mixing 5 minutes (rotating speed 1800rpm) of 50,000,000,000 spores/gram green muscardine fungus xerospore powder, prepare the green muscardine fungus oil-suspending agent that contains 30% (W/W) green muscardine fungus, 15% (W/W) surfactant.Tween solution formulation content with 0.5% (W/V) is 1.0 * 10
7The spore suspension of spore/ml.Worm is prepared with embodiment 2 in examination.Green muscardine fungus oil-suspending agent dilute with water different multiples adopts the distinct device spraying.Adopt POTTER spray tower (BURKARD company) to carry out low-gallonage spraying and ultra low volume spraying (realizing) by regulating spray pressure; Conventional spray adopts manual sprayer.If 30% (W/W) green muscardine fungus oil-suspending agent that does not add surfactant is contrast 1 with ultra low volume spraying after 4 times of the oily soybean salad oil dilutions; 1.0 * 10
7Spore/ml muscardine spore suspension is contrast 2 and not dispenser contrast.Adjusting insectary room temperature to 28～33 ℃, relative moisture 30～60%.Regularly observe every day, each dead borer population of handling of record, and calculate after the dispenser 10 days corrected mortality, repeats 3 times, with the analysis of DPS2000 software statistics.
As can be seen from Table 3: 4 times of product dilute with waters of the present invention and existing green muscardine fungus oil-suspending agent adopt the preventive effect of ultra low volume spraying all more than 90% after diluting 4 times with oil, on the P=0.05 level, do not have significant difference, but water replaces oil to reduce the dispenser cost.Adopt low-gallonage spraying and conventional spray insecticide-applying way control Asiatic migratory locust, the 10th day corrected mortality is higher than 80% after dispenser, all is significantly higher than 1.0 * 10
7Spore/ml muscardine spore water slurry 69.1% corrected mortality illustrates and adopts behind the product dilute with water of the present invention low-gallonage spraying and conventional spray can effectively improve the control efficiency of water slurry.
Table 3 green muscardine fungus oil-suspending agent different administration method is to the virulence test of Asiatic migratory locust
| Handle | Extension rate | Dilution | Spray pattern | Inoculum concentration | Corrected mortality (%) |
| 1 | 4 | Water | Ultra low volume spraying | The 50ul/ cage | 90.4±3.4a |
| Contrast 1 | 4 | Soybean salad oil | Ultra low volume spraying | The 50ul/ cage | 92.9±2.9a |
| 2 | 100 | Water | Low-gallonage spraying | 1.25ml/ cage | 86.9±3.7a |
| 3 | 300 | Water | Low-gallonage spraying | 0.41ml/ cage | 88.3±4.2a |
| 4 | 1000 | Water | Conventional spray | 12.5ml/ cage | 80.6±2.3b |
| 5 | 2000 | Water | Conventional spray | The 25ml/ cage | 81.9±3.2b |
| Contrast 2 | - | - | Conventional spray | 12.5ml/ cage | 69.1±4.6c |
Embodiment 4
The synergistic effect of Trichoderma oil-suspending agent.
The tomato ground that Trichoderma prevents and treats test and Selection infection gray mold (Botrytis cinerea) carries out.(spore content is 10,000,000,000 spores/ml) to contain 30% (W/W) Trichoderma spore oil suspending agent of 10% (W/W) fatty alcohol-polyoxyethylene ether, 5% (W/W) alkylphenol polyoxyethylene by top method with soybean salad oil preparation.Conventional spray (50ml/m behind 1000 times of 30% (W/W) Trichoderma spore oil suspending agent dilute with waters
2), with the Tween solution of 0.5% (W/V) respectively formulation content be the spore Trichoderma suspension of 6.0 * 106 spores/ml do and clear water in contrast.Each handles about 40 strains, repeats 3 times.Spray in per 10 days is once sprayed 2 times continuously.The medicine investigation method is fixed 10 strains for each processing, 10 blades of every strain upper, middle and lower investigation.Investigated disease leaf disease level in 10 days behind the preceding and each spray medicine of spray medicine, and calculate disease index and preventive effect with following formula.
Disease index=∑ [(sick grade blade number * disease level)/blade amt
Disease index * 100 preventive effects (%)=(check plot disease index growth rate-treatment region disease index growth rate)/check plot disease index growth rate * 100 before disease index growth rate (%)=(disease index before control back disease index-control)/control
The killed stage division of tomato leaf: 0 grade: no scab; 1 grade: single blade has 3 of scabs; 3 grades: single blade has 4～6 of scabs; 5 grades: single blade has 7～10 of scabs; 7 grades: single blade has 11～20 of scabs, and part is intensive in flakes; 9 grades: single blade has the intensive leaf area (1～2)/4 that accounts for of scab; 11 grades: single blade has the intensive leaf area (2～3)/4 that accounts for of scab; 13 grades: single blade has the intensive leaf area that accounts for of scab more than 3/4.
The measure of merit of table 4 Trichoderma oil-suspending agent field control graw mold of tomato
As can be seen from Table 4: Trichoderma oil-suspending agent control action to gray mold after twice dispenser improves about 40% and 35% respectively than aqueous suspension agent, illustrates that oil-suspending agent can effectively improve the biologically active of Trichoderma.
Embodiment 5
The synergistic effect of colletotrichum gloeosporioides Penz oil-suspending agent.
Colletotrichum gloeosporioides Penz has the activity of weeds extremely, sprays by plant leaf surface and carries out biological control, and colletotrichum gloeosporioides Penz prevents and treats riotous growth in the identical habitat of test and Selection, and anosis alternanthera philoxeroides (Alternanthera philoxeroides) is as the experimental plot.(spore content is 2,000,000,000 spores/ml) to contain 20% (W/W) colletotrichum gloeosporioides Penz spore oil suspending agent of 10% (W/W) fatty alcohol-polyoxyethylene ether, 5% (W/W) tween by top method with soybean salad oil preparation.Conventional spray (50ml/m behind 2000 times of 20% (W/W) colletotrichum gloeosporioides Penz spore oil suspending agent dilute with waters
2), the water formulation content be the spore Trichoderma suspension of 2.0 * 106 spores/ml do and clear water in contrast.Each handles about 1m
2, repeat 3 times.Adopt 5 point samplings, the occurring degree of 10 leaves of every some random searching is pressed grade scale and is calculated disease index.Disease index=∑ [(sick grade blade number * disease level)/(blade amt * the highest sick level)] * 100.The results are shown in Table 5.
Disease severity grade scale: 0 grade, do not have scab on the blade; 1 grade, 1～4 scab is arranged, not yellow on the blade; 2 grades, blade face scab coverage rate is less than 20%, not yellow; 3 grades, blade face scab coverage rate is less than 40%, not yellow; 4 grades; Blade face scab coverage rate is less than 60%, and scab is intensive, local yellow; 5 grades, blade face scab coverage rate is greater than 80%, and scab gathers or is gathered into large tracts of land necrotic plaque, blade 80% above yellow; 6 grades, blade is to be burnt shape and curls withered or come off; Indoor blade shows as scab and gathers, and is black, rotten shape.
The measure of merit of table 5 colletotrichum gloeosporioides Penz oil-suspending agent field control alternanthera philoxeroides
As can be seen from Table 5: under the effect of the colletotrichum gloeosporioides Penz of same dose, the control efficiency that adopts oil-suspending agent all was significantly higher than aqueous suspension agent in 5 days, 10 days and 15 days after dispenser.
Embodiment 6～21
A kind of fungal spore oil suspending agent, it is characterized in that: suspended oil, surfactant are added in 1 liter the agitator and stir (rotating speed 500rpm), add fungi xerospore powder while stirring, after adding fungi xerospore powder, rotating speed is transferred to 1500rpm to be mixed 2 minutes again, preparation cost invention product fungal spore oil suspending agent, embodiment 6～13 concrete parameters see Table 6, and embodiment 14～21 concrete parameters see Table 7.
Table 6 (by weight, unit is gram)
| Embodiment | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 |
| Green muscardine fungus | 4 | - | - | - | - | 20 | 40 | 40 |
| White muscardine fungi | - | 40 | - | - | - | 20 | 40 | 30 |
| Verticillium lecanii | - | - | 60 | - | - | 100 | 40 | 50 |
| Trichoderma | - | - | - | 80 | - | - | 40 | 30 |
| Colletotrichum gloeosporioides Penz | - | - | - | - | 100 | - | - | 50 |
| Fatty alcohol-polyoxyethylene ether | 4 | - | - | - | 20 | - | 20 | - |
| Alkylphenol polyoxyethylene | - | 40 | - | - | 20 | 10 | 20 | 50 |
| Class of department | - | - | 20 | - | 20 | 20 | 20 | 50 |
| Tween | - | - | - | 40 | - | 30 | 20 | - |
| Soybean oil | 92 | - | - | - | - | - | 160 | - |
| Rapeseed oil | 100 | 60 | - | - | - | - | - | 100 |
| Cotton seed oil | 50 | 60 | 320 | - | - | - | - | - |
| Palm oil | 50 | 50 | - | 280 | - | - | - | - |
| Sesame oil | 40 | 100 | - | - | 240 | - | - | - |
| Corn oil | 60 | 50 | - | - | - | 100 | - | - |
| Fungal spore percentage (W/W) | 1％ | 10％ | 15％ | 20％ | 25％ | 35％ | 40％ | 50％ |
Table 7 (by weight, unit is gram)
| Embodiment | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 |
| Green muscardine fungus | - | 20 | - | - | - | - | - | - |
| White muscardine fungi | 10 | 20 | - | - | 50 | 40 | 30 | 60 |
| Verticillium lecanii | 10 | - | 30 | - | - | 40 | 30 | 60 |
| Trichoderma | - | - | 30 | 40 | 50 | 40 | 40 | - |
| Colletotrichum gloeosporioides Penz | - | - | - | 40 | - | - | 40 | 60 |
| Fatty alcohol-polyoxyethylene ether | 20 | 30 | 10 | - | 10 | 20 | - | - |
| Alkylphenol polyoxyethylene | 20 | - | - | - | - | 30 | 30 | 30 |
| Class of department | - | 30 | - | 30 | 10 | - | - | 20 |
| Tween | - | - | 10 | 30 | 20 | 30 | 30 | 50 |
| Soybean oil | 120 | - | 100 | 100 | - | - | 50 | 40 |
| Rapeseed oil | 140 | 50 | - | 100 | - | - | - | - |
| Cotton seed oil | - | 50 | 100 | 60 | - | 100 | 50 | - |
| Palm oil | - | 100 | 120 | - | 60 | 50 | - | 40 |
| Sesame oil | - | - | - | - | 100 | 50 | - | 40 |
| Corn oil | - | - | - | - | 100 | - | - | - |
| Fungal spore percentage (W/W) | 5％ | 10％ | 15％ | 20％ | 25％ | 30％ | 35％ | 45％ |
Embodiment 22
A kind of fungal spore oil suspending agent, it is characterized in that: the soybean oil of 280g, 16g fatty alcohol-polyoxyethylene ether and 24g alkylphenol polyoxyethylene are added in 1 liter the agitator and stir (rotating speed 500rpm), add 80g while stirring and contain 50,000,000,000 spores/gram green muscardine fungus xerospore powder, after adding conidial powder, rotating speed is transferred to 1500rpm mixed again 2 minutes, be mixed with the green muscardine fungus oil-suspending agent of 20% (W/W).Adopt behind 1 times of the dilute with water and adopt conventional spray after adopting 500 times of the spraying of low capacity technology or dilute with waters behind 10 times of spraying of ultra low volume technology or the dilute with waters.
Embodiment 23
A kind of fungal spore oil suspending agent, it is characterized in that: the rapeseed oil of 280g and 60g fatty alcohol-polyoxyethylene ether are added in 1 liter the agitator and stir (rotating speed 500rpm), add 60g while stirring and contain 30,000,000,000 spores/gram Trichoderma conidial powder, after adding conidial powder, rotating speed is transferred to 1500rpm mixed again 2 minutes, be mixed with the Trichoderma spore oil suspending agent of 15% (W/W).Adopt behind 10 times of the dilute with waters and adopt conventional spray after adopting 3000 times of the spraying of low capacity technology or dilute with waters behind 500 times of spraying of ultra low volume technology or the dilute with waters.
Embodiment 24
A kind of fungal spore oil suspending agent, it is characterized in that: the rapeseed oil of 244g, 32g cotton seed oil, 8g fatty alcohol-polyoxyethylene ether, 12g alkylphenol polyoxyethylene and class of 4g department are added in 1 liter the agitator and stir (rotating speed 500rpm), add 100g while stirring and contain 70,000,000,000 spores/gram white muscardine fungi xerospore powder, after adding conidial powder, rotating speed is transferred to 1500rpm mixed again 2 minutes, be mixed with the white muscardine fungi oil-suspending agent of 25% (W/W).Adopt behind 3 times of the dilute with waters and adopt conventional spray after adopting 1000 times of the spraying of low capacity technology or dilute with waters behind 100 times of spraying of ultra low volume technology or the dilute with waters.
Embodiment 25
A kind of fungal spore oil suspending agent, it is characterized in that: the corn oil of 230Kg, 12g fatty alcohol-polyoxyethylene ether, 16g alkylphenol polyoxyethylene and 2g tween are added in 1 liter the agitator and stir (rotating speed 500rpm), add 140g while stirring and contain 30,000,000,000 spores/gram Verticillium lecanii conidial powder, after adding conidial powder, rotating speed is transferred to 1500rpm mixed again 2 minutes, be mixed with the Verticillium lecanii oil-suspending agent of 30% (W/W).Adopt behind 7 times of the dilute with waters and adopt conventional spray after adopting 2000 times of the spraying of low capacity technology or dilute with waters behind 300 times of spraying of ultra low volume technology or the dilute with waters.
Embodiment 26
A kind of fungal spore oil suspending agent, it is characterized in that: the soybean oil of 160g, 60g fatty alcohol-polyoxyethylene ether and 40g alkylphenol polyoxyethylene are added in 1 liter the agitator and stir (rotating speed 500rpm), add 140g while stirring and contain 10,000,000,000 spores/gram colletotrichum gloeosporioides Penz conidial powder, after adding conidial powder, rotating speed is transferred to 1500rpm mixed again 2 minutes, be mixed with the Verticillium lecanii oil-suspending agent of 35% (W/W).Adopt behind 5 times of the dilute with waters and adopt conventional spray after adopting 1500 times of the spraying of low capacity technology or dilute with waters behind 150 times of spraying of ultra low volume technology or the dilute with waters.
Embodiment 27
A kind of fungal spore oil suspending agent, it is characterized in that: the soybean salad oil of 200g, 40g fatty alcohol-polyoxyethylene ether and 20g alkylphenol polyoxyethylene are added in 1 liter the agitator and stir (rotating speed 500rpm), add while stirring that 100g contains 30,000,000,000 spores/gram Verticillium lecanii spore and 40g contains 70,000,000,000 spores/gram beauveria bassiana spore powder, after adding conidial powder, rotating speed is transferred to 1500rpm to be mixed 2 minutes again, mix, be mixed with the oil-suspending agent that contains 25% (W/W) Verticillium lecanii and 10% (W/W) white muscardine fungi mixed fungus spore.Adopt behind 6 times of the dilute with waters and adopt conventional spray after adopting 2500 times of the spraying of low capacity technology or dilute with waters behind 200 times of spraying of ultra low volume technology or the dilute with waters.
Claims (6)
1, a kind of fungal spore oil suspending agent is characterized in that it is made up of fungal spore powder, surfactant and suspended oil, and wherein the fungal spore powder accounts for 1～50%, surfactant comprise 1～25%, and suspended oil accounts for 25～98%, in weight portion.
2, fungal spore oil suspending agent according to claim 1, wherein the fungal spore powder accounts for 15～35%, surfactant comprise 5～15%, suspended oil accounts for 40～80%, in weight portion.
3, fungal spore oil suspending agent according to claim 1 and 2, described fungal spore are the hydrophily spore or/and the hydrophobicity spore, and described surfactant is a nonionic surface active agent, and described suspended oil is a vegetable oil.
4, fungal spore oil suspending agent according to claim 3, described fungi are green muscardine fungus, white muscardine fungi, Verticillium lecanii, Trichoderma, colletotrichum gloeosporioides Penz or their Mixed Microbes; Described nonionic surface active agent is fatty alcohol-polyoxyethylene ether, alkylphenol polyoxyethylene, class of department, tween or their mixture; Described vegetable oil is soybean oil, rapeseed oil, cotton seed oil, palm oil, sesame oil, corn oil or their mixtures.
5, according to the using method of the described fungal spore oil suspending agent of arbitrary claim in the claim 1～4, it is characterized in that: adopt the spraying of ultra low volume technology behind 1～10 times of the described fungal spore oil suspending agent dilute with water; Or adopt the spraying of low capacity technology behind 10～500 times of the dilute with waters; Or adopt conventional spray behind 500～3000 times of the dilute with waters.
6, adopt the spraying of ultra low volume technology after the using method of fungal spore oil suspending agent according to claim 5,3～7 times of described fungal spore oil suspending agent dilute with waters; Or adopt the spraying of low capacity technology behind 100～300 times of the dilute with waters; Or adopt conventional spray behind 1000～2000 times of the dilute with waters.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100697049A CN101273729B (en) | 2008-05-20 | 2008-05-20 | Fungal spore oil suspending agent |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100697049A CN101273729B (en) | 2008-05-20 | 2008-05-20 | Fungal spore oil suspending agent |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101273729A true CN101273729A (en) | 2008-10-01 |
| CN101273729B CN101273729B (en) | 2011-06-15 |
Family
ID=39993927
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008100697049A Active CN101273729B (en) | 2008-05-20 | 2008-05-20 | Fungal spore oil suspending agent |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101273729B (en) |
Cited By (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101564047B (en) * | 2009-05-27 | 2012-02-15 | 重庆大学 | Disinsection fungi emulsifiable powder |
| CN103975916A (en) * | 2014-05-30 | 2014-08-13 | 东北林业大学 | Dispersible trichoderma asperellum oil suspending agent and preparation method thereof |
| CN104255808A (en) * | 2014-08-25 | 2015-01-07 | 谢明 | Lecanicillium lecanii oil solution, preparing method thereof and applications of the oil solution in agricultural pest control |
| CN104621185A (en) * | 2015-02-16 | 2015-05-20 | 重庆聚立信生物工程有限公司 | Application of metarhizium anisopliae strain in preparation of medicine for killing aphids |
| CN104838892A (en) * | 2015-06-02 | 2015-08-19 | 云南农业大学 | Method for reproducing verticillum lecanii and recovering vigor of verticillum lecanii by using larvas of argyrogramma agnata |
| CN104904752A (en) * | 2015-07-10 | 2015-09-16 | 上海万力华生物科技有限公司 | Trichoderma spore water suspending agent and preparation method thereof |
| CN105053022A (en) * | 2015-08-06 | 2015-11-18 | 陆月霞 | Fungal insecticide |
| CN105567605A (en) * | 2016-02-19 | 2016-05-11 | 黑龙江省科学院大庆分院 | Lysinibacillus sp. and application thereof, degumming auxiliary containing lysinibacillus sp. and preparation method of degumming auxiliary |
| CN105705013A (en) * | 2013-11-08 | 2016-06-22 | 诺维信生物农业公司 | Compositions and methods for treating pests |
| CN105767014A (en) * | 2016-05-11 | 2016-07-20 | 江西正邦生物化工有限责任公司 | Trichoderma-asperellum dispersible oil suspending agent bactericidal composition and preparation method thereof |
| CN106417387A (en) * | 2016-09-23 | 2017-02-22 | 江西天祥通用航空股份有限公司 | Paecilomyces lilacinus oil suspension agent and preparation method |
| CN106489932A (en) * | 2016-10-17 | 2017-03-15 | 上海万力华生物科技有限公司 | A kind of trichoderma oil-suspending agent and preparation method thereof |
| CN106489999A (en) * | 2016-09-23 | 2017-03-15 | 江西天祥通用航空股份有限公司 | One kind is into green meat seat bacterium suspending agent and preparation method thereof |
| CN108402087A (en) * | 2017-02-10 | 2018-08-17 | 湖北省林业科学研究院 | A kind of high cryptogam of muscardine is used for the new method of airplane spray |
| WO2019103588A1 (en) | 2017-11-24 | 2019-05-31 | Université Ibn Tofail | Production, formulation and recycling of a biofungicidal and biostimulant product based on trichoderma asperellum |
| WO2020011819A1 (en) * | 2018-07-10 | 2020-01-16 | Bayer Aktiengesellschaft | Novel carrier fluids for liquid fungal spore formulations |
| CN111657303A (en) * | 2020-07-17 | 2020-09-15 | 唐山市会恩科技有限公司 | Metarhizium anisopliae fungus preparation for killing Spodoptera frugiperda |
| CN112493256A (en) * | 2020-12-03 | 2021-03-16 | 重庆谷百奥生物研究院有限公司 | Metarhizium anisopliae preparation for promoting tomato growth and preventing and treating tomato anthracnose |
| CN112741085A (en) * | 2020-12-30 | 2021-05-04 | 中国农业科学院植物保护研究所 | Trichoderma conidium oil suspending agent and application thereof |
| CN114097796A (en) * | 2021-11-29 | 2022-03-01 | 天津市汉邦植物保护剂有限责任公司 | Dispersible oil suspending agent containing metarhizium anisopliae and cyantraniliprole as well as preparation method and application of dispersible oil suspending agent |
| CN114304188A (en) * | 2021-12-15 | 2022-04-12 | 宁夏大学 | Verticillium lecanii dispersible oil suspending agent and preparation method and application thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX2021012479A (en) * | 2019-04-12 | 2021-11-12 | Danstar Ferment Ag | Methods of increasing the germination rate of fungal spores. |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1168387C (en) * | 2003-03-05 | 2004-09-29 | 湖北省生物农药工程研究中心 | Bacillus thuringiensis oil suspension concentrate pesticide |
| CN100348108C (en) * | 2005-12-13 | 2007-11-14 | 茂名市林业科学研究所 | Muscardine suspension for ultra-low volume spray |
-
2008
- 2008-05-20 CN CN2008100697049A patent/CN101273729B/en active Active
Cited By (29)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101564047B (en) * | 2009-05-27 | 2012-02-15 | 重庆大学 | Disinsection fungi emulsifiable powder |
| CN105705013B (en) * | 2013-11-08 | 2020-01-10 | 诺维信生物农业公司 | Compositions and methods for treating pests |
| CN105705013A (en) * | 2013-11-08 | 2016-06-22 | 诺维信生物农业公司 | Compositions and methods for treating pests |
| US10383339B2 (en) * | 2013-11-08 | 2019-08-20 | Novozymes Bioag A/S | Compositions and methods for treating pests |
| CN103975916A (en) * | 2014-05-30 | 2014-08-13 | 东北林业大学 | Dispersible trichoderma asperellum oil suspending agent and preparation method thereof |
| CN103975916B (en) * | 2014-05-30 | 2015-09-09 | 东北林业大学 | Trichoderma asperellum dispersibles oil-suspending agent and preparation method thereof |
| CN104255808A (en) * | 2014-08-25 | 2015-01-07 | 谢明 | Lecanicillium lecanii oil solution, preparing method thereof and applications of the oil solution in agricultural pest control |
| CN104621185A (en) * | 2015-02-16 | 2015-05-20 | 重庆聚立信生物工程有限公司 | Application of metarhizium anisopliae strain in preparation of medicine for killing aphids |
| CN104621185B (en) * | 2015-02-16 | 2019-02-15 | 重庆聚立信生物工程有限公司 | A kind of application of Metarhizium anisopliae bacterial strain in the medicament for preparing killing aphids |
| CN104838892B (en) * | 2015-06-02 | 2017-04-12 | 云南农业大学 | Method for reproducing verticillum lecanii and recovering vigor of verticillum lecanii by using larvas of argyrogramma agnata |
| CN104838892A (en) * | 2015-06-02 | 2015-08-19 | 云南农业大学 | Method for reproducing verticillum lecanii and recovering vigor of verticillum lecanii by using larvas of argyrogramma agnata |
| CN104904752A (en) * | 2015-07-10 | 2015-09-16 | 上海万力华生物科技有限公司 | Trichoderma spore water suspending agent and preparation method thereof |
| CN105053022A (en) * | 2015-08-06 | 2015-11-18 | 陆月霞 | Fungal insecticide |
| CN105567605A (en) * | 2016-02-19 | 2016-05-11 | 黑龙江省科学院大庆分院 | Lysinibacillus sp. and application thereof, degumming auxiliary containing lysinibacillus sp. and preparation method of degumming auxiliary |
| CN105767014A (en) * | 2016-05-11 | 2016-07-20 | 江西正邦生物化工有限责任公司 | Trichoderma-asperellum dispersible oil suspending agent bactericidal composition and preparation method thereof |
| CN106417387A (en) * | 2016-09-23 | 2017-02-22 | 江西天祥通用航空股份有限公司 | Paecilomyces lilacinus oil suspension agent and preparation method |
| CN106489999A (en) * | 2016-09-23 | 2017-03-15 | 江西天祥通用航空股份有限公司 | One kind is into green meat seat bacterium suspending agent and preparation method thereof |
| CN106489932A (en) * | 2016-10-17 | 2017-03-15 | 上海万力华生物科技有限公司 | A kind of trichoderma oil-suspending agent and preparation method thereof |
| CN108402087A (en) * | 2017-02-10 | 2018-08-17 | 湖北省林业科学研究院 | A kind of high cryptogam of muscardine is used for the new method of airplane spray |
| WO2019103588A1 (en) | 2017-11-24 | 2019-05-31 | Université Ibn Tofail | Production, formulation and recycling of a biofungicidal and biostimulant product based on trichoderma asperellum |
| CN112423584A (en) * | 2018-07-10 | 2021-02-26 | 拜耳公司 | Novel carrier liquid for liquid fungal spore preparations |
| WO2020011819A1 (en) * | 2018-07-10 | 2020-01-16 | Bayer Aktiengesellschaft | Novel carrier fluids for liquid fungal spore formulations |
| CN111657303A (en) * | 2020-07-17 | 2020-09-15 | 唐山市会恩科技有限公司 | Metarhizium anisopliae fungus preparation for killing Spodoptera frugiperda |
| CN112493256A (en) * | 2020-12-03 | 2021-03-16 | 重庆谷百奥生物研究院有限公司 | Metarhizium anisopliae preparation for promoting tomato growth and preventing and treating tomato anthracnose |
| CN112741085A (en) * | 2020-12-30 | 2021-05-04 | 中国农业科学院植物保护研究所 | Trichoderma conidium oil suspending agent and application thereof |
| CN112741085B (en) * | 2020-12-30 | 2022-04-12 | 中国农业科学院植物保护研究所 | Trichoderma conidium oil suspending agent and application thereof |
| CN114097796A (en) * | 2021-11-29 | 2022-03-01 | 天津市汉邦植物保护剂有限责任公司 | Dispersible oil suspending agent containing metarhizium anisopliae and cyantraniliprole as well as preparation method and application of dispersible oil suspending agent |
| CN114304188A (en) * | 2021-12-15 | 2022-04-12 | 宁夏大学 | Verticillium lecanii dispersible oil suspending agent and preparation method and application thereof |
| CN114304188B (en) * | 2021-12-15 | 2023-02-28 | 宁夏大学 | Verticillium lecanii dispersible oil suspending agent and preparation method and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101273729B (en) | 2011-06-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101273729B (en) | Fungal spore oil suspending agent | |
| CN100540656C (en) | A kind of subtilis and microbial inoculum thereof and application | |
| US7429477B2 (en) | Controlling plant pathogens with bacterial/fungal antagonist combinations | |
| CA2471555C (en) | Controlling plant pathogens with fungal/bacterial antagonist combinations comprising trichoderma virens and bacillus amyloliquefaciens | |
| CN101564047B (en) | Disinsection fungi emulsifiable powder | |
| AU2020260526B2 (en) | Clonostachys rosea inoculated plant materials with fungicides and adjuvants | |
| CN101124915B (en) | Biocontrol bacteria compound agent for preventing and controlling crop soil-infectious diseases and preparation method | |
| CN106591185A (en) | Application and preparation of bacillus amyloliquefaciens subsp. plantarum and bacterial agent thereof | |
| Pell et al. | A pheromone trap for dispersal of the pathogen Zoophthora radicans Brefeld.(Zygomycetes: Entomophthorales) amongst populations of the diamondback moth, Plutella xylostella L.(Lepidoptera: Yponomeutidae) | |
| CN100445370C (en) | Bacillus subtilis, bacterium agent and application thereof | |
| Green et al. | Formulating microorganisms for biological control of weeds | |
| CN104039755A (en) | Insecticidal lipid agents isolated from entomopathogenic fungi and uses thereof | |
| CN106070338B (en) | A kind of compounding agricultural insecticidal bactericidal composition and its application | |
| CN103749441A (en) | Isaria fumosorosea wettable powder as well as preparation method and application thereof | |
| CN104531574A (en) | Bacillus amyloliquefaciens gfj-4 and bacillus amyloliquefaciens gfj-4 containing composition | |
| CN104255809A (en) | Isaria fumosorosea oil suspension | |
| CN105875653A (en) | Microbial pesticide containing beauveria bassiana and preparation method of microbial pesticide | |
| CN103783030B (en) | The method of the green ground beetle of a kind of predatory natural enemy insect and green muscardine fungus combination control insect | |
| CN102021122A (en) | High-efficiency insecticidal fungus and applications thereof | |
| CN103461389B (en) | Compound microorganism bacteria agent capable of preventing root-knot nematode and preparation method thereof | |
| CN102172250A (en) | Preparation method and application of eauveria-spinosad suspending agent | |
| CN104988098B (en) | One plant of prevention root rot of beets and the Bacillus strain for promoting Sugarbeet Growth | |
| WO2014193162A1 (en) | Preparation method for biopesticide using paecilomyces sp. strain | |
| CN103749545A (en) | Isaria fumosorosea and bacillus thuringiensis wettable powder | |
| CN102648713B (en) | Technology for improving biological disease-prevention activity of trichoderma preparation by utilizing fungi activator protein |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |