CN106399084A - Probiotic culture system - Google Patents
Probiotic culture system Download PDFInfo
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- CN106399084A CN106399084A CN201610821569.3A CN201610821569A CN106399084A CN 106399084 A CN106399084 A CN 106399084A CN 201610821569 A CN201610821569 A CN 201610821569A CN 106399084 A CN106399084 A CN 106399084A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- Chemical & Material Sciences (AREA)
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- Life Sciences & Earth Sciences (AREA)
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- Bioinformatics & Cheminformatics (AREA)
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- Biomedical Technology (AREA)
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- General Engineering & Computer Science (AREA)
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Abstract
The invention discloses a probiotic culture system. The probiotic culture system comprises a seeding tank, a fermentation tank, an air compression device, a steam generator and a hot water circulation device; a feed port of the seeding tank and a feed port of the fermentation tank are connected with an outlet of the air compression device and an outlet of the steam generator through pipelines; a circulation pump is installed between a discharge port formed in the bottom of the seeding tank and the feed port and a circulation port which are formed in the upper portion of the seeding tank, and a circulation pump is installed between a discharge port formed in the bottom of the fermentation tank and the feed port and a circulation port which are formed in the upper portion of the fermentation tank; a transmission pipeline is connected between the discharge port of the seeding tank and the feed port of the fermentation tank; hot water circulation channels are formed in the wall of a seeding tank body and the wall of a fermentation tank body respectively and connected with the hot water circulation device to form a hot water circulation loop. The probiotic culture system has the advantages that complete supporting facilities are achieved, the structure is simplified, the performance is improved, the probiotic production requirements are met step by step, and the equipment investment can be greatly reduced, so that the price of probiotic products is obviously reduced.
Description
Technical field
The present invention relates to a kind of culture systems of probiotic bacteria, belong to culture and the fermentation arts of probiotic bacteria.
Background technology
Probiotic bacteria has application in food and medicine, and the Zymolysis Equipment of current probiotic bacteria is typically all import, import
Device structure is complicated, expensive, causes product cost to be greatly improved.Therefore, a kind of structure of development is simple, it is low to prepare,
The culture systems of the probiotic bacteria of excellent performance will have important market application foreground.
Content of the invention
The present invention is intended to provide a kind of probiotic's culture system, answered with the device structure solving the import of prior art presence
Miscellaneous, expensive, cause the problem that product cost is greatly improved.
In order to solve above-mentioned technical problem, the technical solution used in the present invention is：
A kind of probiotic's culture system, follows including seeding tank, fermentation tank, blowing plant, steam generator and hot water
Loop device, the charging aperture of described seeding tank and fermentation tank is passed through pipeline and is connected with the outlet of blowing plant and steam generator
Connect；Equipped with circulating pump between the discharging opening of described seeding tank and fermenter base and the charging aperture on top and circulation port；?
The discharging opening of this seeding tank is connected with transmission pipeline to the charging aperture of fermentation tank；Described seeding tank and fermentation tank tank wall
It is provided with hot water circuit passage, and be connected composition hot water circulation loop with hot water circulating device.
Wherein, described seeding tank is identical with fermentation jar structure, and the volume of fermentation tank is more than the volume of seeding tank；Described
Charging aperture is connected with the top of the axial duct being located at tank interior, the bottom of this axial duct extend to tank body bottom and with
One circulating line connects, and following in this circulating line is evenly equipped with multiple apertures；The tank interior part of described circulation port is to side
Tilt.
It is provided with manhole, respiratory organ interface, rotating wash bowl interface (CIP), pressure gauge connection at the top of described tank body and regard
Mirror interface；The discharging opening of the bottom of this tank body is provided with sample tap；It is provided with compressed air inlet on the top of this tank body,
The inner of this compressed air inlet is connected with the top of described axial duct；It is provided with thermometer interface in the side of this tank body.
Described tank body includes upper cover, inner barrel, heat-insulation layer, exoperidium and low head, the top and bottom of inner barrel
It is connected with upper cover and low head respectively, in the outside spacers setting exoperidium of this inner barrel and low head, be close to this
The outside of inner barrel and low head is provided with hot water circuit passage, filling heat insulating wall between hot water circuit passage and exoperidium；?
The bottom of this tank body and top are respectively equipped with hot water inlet and hot water outlet, and this hot water inlet and hot water outlet are followed with hot water respectively
The two ends of ring passage communicate, and are connected with described hot water circulating device.
Preferably, the bottom in described tank body is provided with multiple legs.
Preferably, described blowing plant includes air compressor and air accumulator, and the outlet of air compressor is passed through
Pipeline is connected with the entrance of air accumulator, and the outlet of air accumulator is passed through pipeline and connected with the charging aperture of described seeding tank and fermentation tank
Connect.
Preferably, described hot water circulating device includes hot-water cylinder, hot water circulating pump and hot water pipeline, the heat being serially connected
Water pot and hot water circulating pump are connected as by the hot water circuit passage of hot water pipeline and described seeding tank and fermentation tank tank wall
Closed circuit；Described hot-water cylinder is heated using the electrical bar in insertion tank body lower part, and equipped with temperature regulating device.
Main advantages of the present invention include：Comprehensive, structure simplifies, and performance improves, and stepping meets the life of probiotic bacteria
Producing and require, also can substantially reduce equipment investment, thus substantially reducing the price of probiotic products, providing thing beautiful for consumers in general
Inexpensive probiotic products.
Brief description
Fig. 1 is the overall composition schematic diagram of the present invention；
Fig. 2 is the structural representation of the hot-water cylinder of the present invention；
Fig. 3 is the top view of Fig. 2；
Fig. 4 is the structural representation of seeding tank in present system and fermentation tank；
Fig. 5 is the schematic top plan view of Fig. 4；
Fig. 6 is the W direction view (locally) in Fig. 4.
Description of reference numerals：1st, upper cover, 2, inner barrel, 3, heat-insulation layer, 4, exoperidium, 5, acidometer interface, 6, lower envelope
Head, 7, leg, 8, axial duct, 81, (justifying) circulating line, 9, circulation interface, 10, hot water circuit passage, 12, circulating pump, 13,
Entrance three-way change-over valve, 14, outlet three-way change-over valve, 15, circulating line, 16, hot water circulating pump, 17, valve is (in order to avoid vapor
Enter air accumulator), 21, hot-water cylinder, 210, cylinder cover, 211, crossbeam, 212, inner barrel, 213, heat-insulation layer, 214, outer cylinder body,
215th, heat the mouth of pipe, 216, under-chassis, 217, inlet, 218, liquid outlet, 219, temperature sensor interface；22nd, fermentation tank, 23,
Seeding tank, 24, air accumulator, 25, steam generator, 26, air compressor, 27, hot water pipeline, 28, air and steam pipe
Road, 29, compressed air piping, 30 jet chimneys, 32, three-level tank body air vent, C1, CIP interface, E, visor interface, K1-K18,
Pipeline valve, M, manhole, N1, charging aperture, N2, respiratory organ interface, N3, standby mouth, M, hot water outlet, N5, hot water inlet, N6,
Discharging opening, N7, compressed air inlet, N8, sample tap, T, thermometer interface, P, pressure gauge connection.
Specific embodiment
To further describe the present invention with reference to specific embodiment, advantages of the present invention and feature will be with description and
Apparent.It should be understood that described embodiment is only exemplary, any restriction is not constituted to the scope of the present invention.This area
Technical staff should be understood that lower without departing from the spirit and scope of the present invention can to the details of technical solution of the present invention and
Form is modified or is replaced, but these modifications or replacement each fall within protection scope of the present invention.
Referring to Fig. 1-Fig. 6, a kind of probiotic's culture system provided by the present invention, including seeding tank 23, fermentation tank 22, pressure
Contracting air assembly, steam generator 25 and hot water circulating device, the charging aperture N1 of described seeding tank 23 and fermentation tank 22 passes through
Pipeline is connected with the outlet of blowing plant and steam generator 25；In going out of described seeding tank 23 and fermentation tank 22 bottom
Equipped with circulating pump 12 between the charging aperture N1 on material mouth N6 and top and circulation port 9；Extremely ferment in the discharging opening N6 of this seeding tank 23
It is connected with transmission pipeline (due in figure lines too much, therefore not shown) between the charging aperture N1 of tank 22；Described seeding tank 23 He
Fermentation tank 22 tank wall is provided with hot water circuit passage, and is connected composition hot water circulation loop with hot water circulating device.
Described blowing plant includes air compressor 26 and air accumulator 24, and pipe is passed through in the outlet of air compressor 26
Road is connected with the entrance of air accumulator 24, and the charging of pipeline and described seeding tank 23 and fermentation tank 22 is passed through in the outlet of air accumulator 24
Mouth N1 connects.
Referring to Fig. 2 and Fig. 3, described hot water circulating device includes hot-water cylinder 21, hot water circulating pump 16 and hot water pipeline 27,
The hot-water cylinder 21 being serially connected and hot water circulating pump 16 pass through hot water pipeline 27 and described seeding tank 23 and fermentation tank 22 tank body
The hot water circuit passage of wall is connected as closed circuit.
Described hot-water cylinder 21 includes cylinder and cylinder cover 210, and described cylinder by inner barrel 212, outer cylinder body 214 and is protected
Warm layer 213 forms, and is provided with heat-insulation layer 213 between inner barrel 212 and outer cylinder body 214；This cylinder cover 210 is provided with inlet
217 and temperature sensor interface 219, it is provided with liquid outlet 218 in the bottom of this cylinder, be circumferentially provided with the bottom of this cylinder many
The individual heating mouth of pipe 215.Crossbeam 211 is provided with cylinder cover 210.It is provided with four under-chassis 216 in the bottom of described cylinder.Described
The bottom of cylinder tilt to described liquid outlet 218.Described inner barrel 212, outer cylinder body 214 and cylinder cover 210 all adopt
304 rustless steels are made.
Referring to Fig. 4-Fig. 6, described seeding tank 23 is identical with fermentation tank 22 structure, and the volume of the latter is more than the former；Described
Charging aperture N1 be connected with the top of the axial duct 8 being located within tank body A, the bottom of this axial duct 8 extends to tank body A's
Bottom is simultaneously connected with a circulating line 81, and following in this circulating line 81 is evenly equipped with multiple apertures；The tank of described circulation port 9
Interior part is to lopsidedness.The inner of the circulation port 9 on tank body top to lopsidedness (non-radial, put down with the tangent line of tank body circumference
OK), the bacterium solution of circulation is injected in tank body with the direction of near-tangential, makes bacterium solution in tank do circle and rotates, plays in agitator tank
The effect of bacterium solution；By rustless steel circulating line 81 aperture that Open Side Down that ttom of pipe is circular, bacterium solution is gone out from aperture, can
Tank bottoms to impact, and return bacterium solution surface from tank bottoms, so that bacterium solution in tank is done and stir up and down, play the work of bacterium solution in agitator tank
With substantially increasing the uniformity of bacterium solution, improve ferment effect and quality.
It is provided with manhole M, respiratory organ interface N2, CIP interface C1, pressure gauge connection P and visor at the top of described tank body A
Interface E；The discharging opening N6 of the bottom of this tank body A is provided with sample tap N8；It is provided with compressed air on the top of this tank body A
Import N7, the inner of this compressed air inlet N7 is connected with the top of described axial duct 8；It is provided with the side of this tank body A
Thermometer interface T.
Described tank body A includes upper cover 1, inner barrel 2, heat-insulation layer 3, exoperidium 4 and low head 6, the upper end of inner barrel 2
It is connected with upper cover 1 and low head 6 respectively with lower end, exoperidium 4 is set in the outside spacers of this inner barrel 2 and low head 6,
It is provided with hot water circuit passage 10 in the outside being close to this inner barrel 2 and low head 6, in hot water circuit passage 10 and exoperidium 4
Between filling heat insulating wall 3；It is respectively equipped with hot water inlet N5 and hot water outlet N4, this hot water inlet in the bottom of this tank body and top
The two ends of N5 and hot water outlet N4 hot water circuit passage 10 respectively communicate, and are connected with described hot water circulating device.
It is provided with multiple legs 7 in the bottom of described tank body A.The hot-water cylinder 21 of present system, seeding tank 23 and gas storage
Tank 24 typical volume is 1000L, and fermentation tank 22 is 5500L.
Present system in the running, taken out bacterium solution from tank base by circulating pump 12 by fermentation tank 22 and seeding tank 23
Go out, deliver to and in tank, carry out self-loopa by the charging aperture N1 and circulation port 9 on top.Seeding tank 23 discharging opening is entered with fermentation tank 22
Material mouth is connected by transmission pipeline, couples vacuum pump by k7 valve pipe and takes out air from fermentation tank 22, reduces fermentation tank 22
In air pressure, strain pressure differential effect under from seed tank 23 pass through pipeline flow-direction fermentation tank 22 in, decrease pollution risk.
K1, k2 are running water valve；K3, k4, k5 are expanding propagation tank circulation valve；K6 be vapor valve, be mainly used in for
Expanding propagation tank sterilizes；K7, k8 are burp valve, and k7 end is associated with vacuum pumping pump, reduces expanding propagation tank internal gas pressure, and seed tank between
Form pressure differential, strain is pumped into level Four tank from three-level tank, reduce pollution；K9 is hot water inlet's valve；K10, k11 add water to go out
Mouth valve；K12, k13 are sterilizing steam valve；K14, k15, k16 are seed tank circulation valve；My gas storage tank valve of k17, in order to avoid
Vapor enters air accumulator；K18 is vapor pipeline valve, in order to avoid pressure-air enters in steam generator.
Claims (8)
1. a kind of probiotic's culture system, including seeding tank (23), fermentation tank (22), blowing plant, steam generator
(25) and hot water circulating device it is characterised in that described seeding tank (23) and fermentation tank (22) charging aperture (N1) pass through pipe
Road is connected with the outlet of blowing plant and steam generator (25)；In described seeding tank (23) and fermentation tank (22) bottom
Discharging opening (N6) and the charging aperture (N1) on top and circulation port (9) between equipped with circulating pump (12)；In this seeding tank (23)
Discharging opening (N6) is connected with transmission pipeline to the charging aperture (N1) of fermentation tank (22)；Described seeding tank (23) and fermentation tank
(22) tank wall is provided with hot water circuit passage, and is connected composition hot water circulation loop with hot water circulating device.
2. probiotic's culture system according to claim 1 is it is characterised in that described seeding tank (23) and fermentation tank
(22) structure is identical, and the volume of fermentation tank (22) is more than the volume of seeding tank (23)；Described charging aperture (N1) be located at tank body
(A) top of internal axial duct (8) connects, the bottom of this axial duct (8) extend to tank body (A) bottom and with a ring
Shape pipeline (81) connects, and following in this circulating line (81) is evenly equipped with multiple apertures；The tank interior part of described circulation port (9)
To lopsidedness.
3. probiotic's culture system according to claim 2 is it is characterised in that be provided with the top of described tank body (A)
Manhole (M), respiratory organ interface (N2), rotating wash bowl interface (C1), pressure gauge connection (P) and visor interface (E)；At this tank body (A)
The discharging opening (N6) of bottom be provided with sample tap (N8)；It is provided with compressed air inlet (N7) on the top of this tank body (A),
The inner of this compressed air inlet (N7) is connected with the top of described axial duct (8)；It is provided with temperature in the side of this tank body (A)
Degree table interface (T).
4. probiotic's culture system according to claim 2 is it is characterised in that described tank body (A) includes upper cover
(1), inner barrel (2), heat-insulation layer (3), exoperidium (4) and low head (6), the top and bottom of inner barrel (2) respectively with upper envelope
Head (1) and low head (6) connect, and in outside spacers setting exoperidium (4) of this inner barrel (2) and low head (6), are being close to
It is provided with hot water circuit passage (10) in the outside of this inner barrel (2) and low head (6), in hot water circuit passage (10) and exoperidium
(4) filling heat insulating wall (3) between；It is respectively equipped with hot water inlet (N5) and hot water outlet (N4) in the bottom of this tank body and top,
This hot water inlet (N5) and hot water outlet (N4) are communicated with the two ends of hot water circuit passage (10) respectively, and follow with described hot water
Loop device connects.
5. probiotic's culture system according to claim 2 is it is characterised in that be provided with the bottom of described tank body (A)
Multiple legs (7).
6. probiotic's culture system according to claim 1 is it is characterised in that described blowing plant includes air
Compressor (26) and air accumulator (24), the outlet of air compressor (26) is connected with the entrance of air accumulator (24) by pipeline, storage
The outlet of gas tank (24) is connected with the charging aperture (N1) of described seeding tank (23) and fermentation tank (22) by pipeline.
7. probiotic's culture system according to claim 1 is it is characterised in that described hot water circulating device includes hot water
Tank (21), hot water circulating pump (16) and hot water pipeline (27), the hot-water cylinder being serially connected (21) and hot water circulating pump (16) pass through
Hot water pipeline (27) is connected as closed circuit with the hot water circuit passage of described seeding tank (23) and fermentation tank (22) tank wall.
8. probiotic's culture system according to claim 7 is it is characterised in that described hot-water cylinder (21) is using insertion tank
Electrical bar heating in body bottom, and equipped with temperature regulating device.
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| CN201610821569.3A CN106399084B (en) | 2016-09-14 | 2016-09-14 | Probiotic's culture system |
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| CN201610821569.3A CN106399084B (en) | 2016-09-14 | 2016-09-14 | Probiotic's culture system |
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Cited By (5)
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| CN106881058A (en) * | 2017-04-06 | 2017-06-23 | 山东泰邦生物制品有限公司 | A kind of full-automatic solid-liquid separation system and preparation method thereof |
| CN107254402A (en) * | 2017-06-27 | 2017-10-17 | 江苏益玛生物科技有限公司 | A kind of agar medium preparation system |
| CN107541532A (en) * | 2017-09-29 | 2018-01-05 | 迪沙药业集团有限公司 | A kind of preparation method of the carboxylic acid of 5 methylpyrazine 2 |
| CN109294889A (en) * | 2018-08-31 | 2019-02-01 | 南通劲凌智能科技有限公司 | Probiotics fermented beverage fermentation tank, component proportion and its process |
| CN110195013A (en) * | 2019-06-21 | 2019-09-03 | 李凤兰 | A kind of anaerobic bacteria culture system |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109294889B (en) * | 2018-08-31 | 2021-10-15 | 杭州百芮生物科技有限公司 | Probiotic fermented beverage fermentation tank, component proportion and process method thereof |
| CN110195013A (en) * | 2019-06-21 | 2019-09-03 | 李凤兰 | A kind of anaerobic bacteria culture system |
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| CN106399084B (en) | 2019-05-28 |
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