CN106399084B - Probiotic's culture system - Google Patents
Probiotic's culture system Download PDFInfo
- Publication number
- CN106399084B CN106399084B CN201610821569.3A CN201610821569A CN106399084B CN 106399084 B CN106399084 B CN 106399084B CN 201610821569 A CN201610821569 A CN 201610821569A CN 106399084 B CN106399084 B CN 106399084B
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- Prior art keywords
- hot water
- tank
- fermentor
- probiotic
- tank body
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
Abstract
The invention discloses a kind of probiotic's culture systems, including seeding tank, fermentor, blowing plant, steam generator and hot water circulating device, the feed inlet of the seeding tank and fermentor is connect by pipeline with the outlet of blowing plant and steam generator；Circulating pump is housed between the discharge port of seeding tank and fermenter base and the feed inlet on top and circulation port；Transmission pipeline is connected between feed inlet of the discharge port to fermentor of the seeding tank；The seeding tank and fermentor tank wall is equipped with hot water circuit channel, and composition hot water circulation loop is connect with hot water circulating device.The invention has the advantages that comprehensive, structure simplifies, and performance improves, and stepping meets the production requirement of probiotics, can also substantially reduce equipment investment, to be substantially reduced the price of probiotic products.
Description
Technical field
The present invention relates to a kind of culture systems of probiotics, belong to culture and the fermentation arts of probiotics.
Background technique
Probiotics has application in food and drug, and the Zymolysis Equipment of probiotics is typically all import at present, import
Device structure is complicated, expensive, and product cost is caused to greatly improve.Therefore, develop a kind of structure is simple, it is low to prepare,
The culture systems for the probiotics haveing excellent performance will be with important market application prospect.
Summary of the invention
The present invention is intended to provide a kind of probiotic's culture system, multiple with the device structure for solving import of the existing technology
It is miscellaneous, expensive, the problem of causing product cost to greatly improve.
In order to solve the above-mentioned technical problem, the technical solution used in the present invention is:
A kind of probiotic's culture system, including seeding tank, fermentor, blowing plant, steam generator and hot water follow
The feed inlet of loop device, the seeding tank and fermentor is connected by the outlet of pipeline and blowing plant and steam generator
It connects；Circulating pump is housed between the discharge port of seeding tank and fermenter base and the feed inlet on top and circulation port；?
The discharge port of the seeding tank is connected with transmission pipeline between the feed inlet of fermentor；The seeding tank and fermentor tank wall
Equipped with hot water circuit channel, and composition hot water circulation loop is connect with hot water circulating device.
Wherein, the seeding tank is identical with fermentation jar structure, and the volume of fermentor is greater than the volume of seeding tank；Described
Feed inlet is connect with the top for the axial duct for being located at tank interior, the bottom end of the axial duct extend to the bottom of tank body and with
The connection of one circulating line, is evenly equipped with multiple apertures below the circulating line；The tank inner part of the circulation port is to side
Inclination.
Manhole, respirator interface, rotating wash bowl interface (CIP), pressure gauge connection and view are equipped at the top of the tank body
Mirror interface；Sample tap is equipped on the discharge port of the bottom of the tank body；It is equipped with compressed air inlet on the top of the tank body,
The inner end of the compressed air inlet is connected to the top of the axial duct；Thermometer interface is equipped in the side of the tank body.
The tank body includes upper cover, inner cylinder, insulating layer, exoperidium and lower head, the top and bottom of inner cylinder
It is connect respectively with upper cover and lower head, in the outside spacers setting exoperidium of the inner cylinder and lower head, is being tightly attached to this
The outside of inner cylinder and lower head is equipped with hot water circuit channel, the filling heat insulating wall between hot water circuit channel and exoperidium；?
The bottom and top of the tank body are respectively equipped with hot water inlet and hot water outlet, and the hot water inlet and hot water outlet are followed with hot water respectively
The both ends in ring channel communicate, and connect with the hot water circulating device.
Preferably, multiple stabilizer blades are equipped in the bottom end of the tank body.
Preferably, the blowing plant includes air compressor and air accumulator, and the outlet of air compressor passes through
The outlet of the connection of the entrance of pipeline and air accumulator, air accumulator is connected by the feed inlet of pipeline and the seeding tank and fermentor
It connects.
Preferably, the hot water circulating device includes hot-water cylinder, hot water circulating pump and hot water pipeline, the heat being serially connected
Water pot and hot water circulating pump are connected as by the hot water circuit channel of hot water pipeline and the seeding tank and fermentor tank wall
Circulation loop；The hot-water cylinder is equipped with temperature regulating device using the electrical bar heating in insertion tank body lower part.
Main advantages of the present invention include: it is comprehensive, structure simplifies, and performance improves, and stepping meets the life of probiotics
It produces and requires, can also substantially reduce equipment investment, to be substantially reduced the price of probiotic products, provide object beauty for the majority of consumers
Inexpensive probiotic products.
Detailed description of the invention
Fig. 1 is overall composition schematic diagram of the invention；
Fig. 2 is the structural schematic diagram of hot-water cylinder of the invention；
Fig. 3 is the top view of Fig. 2；
Fig. 4 is the structural schematic diagram of the seeding tank and fermentor in present system；
Fig. 5 is the schematic top plan view of Fig. 4；
Fig. 6 is the W direction view (part) in Fig. 4.
Description of symbols: 1, upper cover, 2, inner cylinder, 3, insulating layer, 4, exoperidium, 5, acidometer interface, 6, lower envelope
Head, 7, stabilizer blade, 8, axial duct, 81, (circle) circulating line, 9, circulation interface, 10, hot water circuit channel, 12, circulating pump, 13,
Entrance three-way change-over valve, 14, outlet three-way change-over valve, 15, circulating line, 16, hot water circulating pump, 17, valve is (in order to avoid vapor
Into air accumulator), 21, hot-water cylinder, 210, cylinder cover, 211, crossbeam, 212, inner cylinder, 213, insulating layer, 214, outer barrel,
215, nozzle, 216, under-chassis, 217, inlet, 218, liquid outlet, 219, temperature sensor interface are heated；22, fermentor, 23,
Seeding tank, 24, air accumulator, 25, steam generator, 26, air compressor, 27, hot water pipeline, 28, air and steam mixing tube
Road, 29, compressed air pipe, 30 jet chimneys, 32, three-level tank exhaust mouth, C1, CIP interface, E, visor interface, K1-K18,
Pipeline valve, M, manhole, N1, feed inlet, N2, respirator interface, N3, spare mouth, M, hot water outlet, N5, hot water inlet, N6,
Discharge port, N7, compressed air inlet, N8, sample tap, T, thermometer interface, P, pressure gauge connection.
Specific embodiment
The invention will now be further described with reference to specific embodiments, the advantages and features of the present invention will be with description and
It is apparent.It should be understood that described, examples are merely exemplary, does not constitute any restrictions to the scope of the present invention.This field
Technical staff should be understood that without departing from the spirit and scope of the invention can details to technical solution of the present invention and
Form is modified or is replaced, but these modifications or substitutions each fall within protection scope of the present invention.
Referring to Fig. 1-Fig. 6, a kind of probiotic's culture system provided by the present invention, including seeding tank 23, fermentor 22, pressure
The feed inlet N1 of contracting air assembly, steam generator 25 and hot water circulating device, the seeding tank 23 and fermentor 22 passes through
Pipeline is connect with the outlet of blowing plant and steam generator 25；In going out for 22 bottom of the seeding tank 23 and fermentor
Circulating pump 12 is housed between material mouth N6 and the feed inlet N1 and circulation port 9 on top；It extremely ferments in the discharge port N6 of the seeding tank 23
Transmission pipeline (since lines are too many in figure, therefore not shown) is connected between the feed inlet N1 of tank 22；23 He of seeding tank
22 tank wall of fermentor is equipped with hot water circuit channel, and composition hot water circulation loop is connect with hot water circulating device.
The blowing plant includes air compressor 26 and air accumulator 24, and the outlet of air compressor 26 passes through pipe
Road is connect with the entrance of air accumulator 24, the charging that the outlet of air accumulator 24 passes through pipeline and the seeding tank 23 and fermentor 22
Mouth N1 connection.
Referring to figs. 2 and 3, the hot water circulating device includes hot-water cylinder 21, hot water circulating pump 16 and hot water pipeline 27,
The hot-water cylinder 21 and hot water circulating pump 16 being serially connected pass through hot water pipeline 27 and 22 tank body of the seeding tank 23 and fermentor
The hot water circuit channel of wall is connected as circulation loop.
The hot-water cylinder 21 includes cylinder and cylinder cover 210, and the cylinder is by inner cylinder 212, outer barrel 214 and protects
Warm layer 213 forms, and insulating layer 213 is equipped between inner cylinder 212 and outer barrel 214；Inlet is equipped on the cylinder cover 210
217 and temperature sensor interface 219, it is equipped with liquid outlet 218 in the bottom of the cylinder, is circumferentially equipped in the lower part of the cylinder more
A heating nozzle 215.Crossbeam 211 is equipped on cylinder cover 210.Under-chassis 216 there are four being set in the bottom of the cylinder.It is described
The bottom of cylinder tilted to the liquid outlet 218.Inner cylinder 212, outer barrel 214 and the cylinder cover 210 is all made of
304 stainless steels are made.
- Fig. 6 referring to fig. 4, the seeding tank 23 is identical with 22 structure of fermentor, and the volume of the latter is greater than the former；It is described
Feed inlet N1 connect with the top for the axial duct 8 being located inside tank body A, the bottom end of the axial duct 8 extends to tank body A's
Bottom is simultaneously connect with a circulating line 81, is evenly equipped with multiple apertures below the circulating line 81；The tank of the circulation port 9
Inner part is tilted to side.The inner end of the circulation port 9 on tank body top tilt to side (it is non-radial, it is flat with the tangent line of tank body circumference
Row), the bacterium solution of circulation is injected into tank body with the direction of near-tangential, so that bacterium solution in tank is done circle rotation, is played in agitator tank
The effect of bacterium solution；By the aperture that Open Side Down of stainless steel circulating line 81 of tube bottom annular shape, bacterium solution is gone out from aperture, can
It to impact tank bottom, and returns from tank bottom to bacterium solution surface, does bacterium solution and stir up and down, play the work of bacterium solution in agitator tank
With substantially increasing the uniformity of bacterium solution, improve ferment effect and quality.
Manhole M, respirator interface N2, CIP interface C1, pressure gauge connection P and visor are equipped at the top of the tank body A
Interface E；Sample tap N8 is equipped on the discharge port N6 of the bottom of tank body A；Compressed air is equipped on the top of tank body A
Import N7, the inner end of compressed air inlet N7 are connected to the top of the axial duct 8；It is equipped in the side of tank body A
Thermometer interface T.
The tank body A includes upper cover 1, inner cylinder 2, insulating layer 3, exoperidium 4 and lower head 6, the upper end of inner cylinder 2
It is connect respectively with upper cover 1 and lower head 6 with lower end, exoperidium 4 is set in the outside spacers of the inner cylinder 2 and lower head 6,
It is equipped with hot water circuit channel 10 in the outside for being tightly attached to the inner cylinder 2 and lower head 6, in hot water circuit channel 10 and exoperidium 4
Between filling heat insulating wall 3；It is respectively equipped with hot water inlet N5 and hot water outlet N4 in the bottom of the tank body and top, the hot water inlet
The both ends in the hot water circuit channel 10 respectively N5 and hot water outlet N4 communicate, and connect with the hot water circulating device.
Multiple stabilizer blades 7 are equipped in the bottom end of the tank body A.Hot-water cylinder 21, seeding tank 23 and the gas storage of present system
24 typical volume of tank is 1000L, and fermentor 22 is 5500L.
Present system in the running, take out bacterium solution from tank base by circulating pump 12 by fermentor 22 and seeding tank 23
Out, it is sent in tank by the feed inlet N1 and circulation port 9 on top and carries out self-loopa.23 discharge port of seeding tank and fermentor 22 into
Material mouth is connected by transmission pipeline, is coupled vacuum pump by k7 valve pipe and is taken out air from fermentor 22, reduces fermentor 22
In air pressure, strain is under pressure difference effect from seeding tank 23 by reducing pollution risk in pipeline flow-direction fermentor 22.
K1, k2 are running water valve；K3, k4, k5 are to expand numerous tank circulation valve；K6 be vapor valve, be mainly used for for
Expand numerous tank sterilizing；K7, k8 are burp valve, and the end k7 is associated with vacuum pumping pump, reduce and expand in numerous tank between air pressure and seeding tank
Pressure difference is formed, strain is pumped into level Four tank from three-level tank, reduces pollution；K9 is hot water inlet's valve；K10, k11 are added water and go out
Mouth valve；K12, k13 are sterilizing steam valve；K14, k15, k16 are seeding tank circulation valve；K17 is gas storage tank valve, in order to avoid
Vapor enters air accumulator；K18 is vapor pipeline valve, in case pressure-air enters in steam generator.
Claims (8)
1. a kind of probiotic's culture system, including seeding tank (23), fermentor (22), blowing plant, steam generator
(25) and hot water circulating device, which is characterized in that the feed inlet (N1) of the seeding tank (23) and fermentor (22) passes through pipe
Road is connect with the outlet of blowing plant and steam generator (25)；In the seeding tank (23) and fermentor (22) bottom
Discharge port (N6) and top feed inlet (N1) and circulation port (9) between be equipped with circulating pump (12)；In the seeding tank (23)
Discharge port (N6) is connected with transmission pipeline between the feed inlet (N1) of fermentor (22)；The seeding tank (23) and fermentor
(22) tank wall is equipped with hot water circuit channel, and composition hot water circulation loop is connect with hot water circulating device；
The top of the feed inlet (N1) axial duct (8) internal with the tank body (A) for being located at fermentor and seeding tank is connect,
The bottom end of the axial duct (8) extends to the bottom of tank body (A) and connect with a circulating line (81), in the circulating line (81)
Below be evenly equipped with multiple apertures；The tank inner part of the circulation port (9) is tilted to side, flat with the tangent line of tank body circumference
Row.
2. probiotic's culture system according to claim 1, which is characterized in that the seeding tank (23) and fermentor
(22) structure is identical, and the volume of fermentor (22) is greater than the volume of seeding tank (23).
3. probiotic's culture system according to claim 2, which is characterized in that be equipped at the top of the tank body (A)
Manhole (M), respirator interface (N2), CIP interface (C1), pressure gauge connection (P) and visor interface (E)；At the bottom of the tank body (A)
The discharge port (N6) in portion is equipped with sample tap (N8)；It is equipped with compressed air inlet (N7) on the top of the tank body (A), the pressure
The inner end of contracting air intlet (N7) is connected to the top of the axial duct (8)；Thermometer is equipped in the side of the tank body (A)
Interface (T).
4. probiotic's culture system according to claim 2, which is characterized in that the tank body (A) includes upper cover
(1), inner cylinder (2), insulating layer (3), exoperidium (4) and lower head (6), the top and bottom of inner cylinder (2) respectively with upper envelope
Head (1) and lower head (6) connection are arranged exoperidium (4) in the outside spacers of the inner cylinder (2) and lower head (6), are being tightly attached to
The outside of the inner cylinder (2) and lower head (6) is equipped with hot water circuit channel (10), in hot water circuit channel (10) and exoperidium
(4) filling heat insulating wall (3) between；It is respectively equipped with hot water inlet (N5) and hot water outlet (N4) in the bottom of the tank body and top,
The hot water inlet (N5) and hot water outlet (N4) are communicated with the both ends of hot water circuit channel (10) respectively, and are followed with the hot water
Loop device connection.
5. probiotic's culture system according to claim 2, which is characterized in that be equipped in the bottom end of the tank body (A)
Multiple stabilizer blades (7).
6. probiotic's culture system according to claim 1, which is characterized in that the blowing plant includes air
The outlet of compressor (26) and air accumulator (24), air compressor (26) is connect by pipeline with the entrance of air accumulator (24), is stored up
The outlet of gas tank (24) is connect by pipeline with the feed inlet (N1) of the seeding tank (23) and fermentor (22).
7. probiotic's culture system according to claim 1, which is characterized in that the hot water circulating device includes hot water
Tank (21), hot water circulating pump (16) and hot water pipeline (27), the hot-water cylinder being serially connected (21) and hot water circulating pump (16) pass through
The hot water circuit channel of hot water pipeline (27) and the seeding tank (23) and fermentor (22) tank wall is connected as circulation loop.
8. probiotic's culture system according to claim 7, which is characterized in that the hot-water cylinder (21) is using insertion tank
Electrical bar heating in body lower part, and temperature regulating device is housed.
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| CN201610821569.3A CN106399084B (en) | 2016-09-14 | 2016-09-14 | Probiotic's culture system |
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| CN201610821569.3A CN106399084B (en) | 2016-09-14 | 2016-09-14 | Probiotic's culture system |
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| CN106399084B true CN106399084B (en) | 2019-05-28 |
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| CN106881058B (en) * | 2017-04-06 | 2023-02-03 | 山东泰邦生物制品有限公司 | Full-automatic solid-liquid separation and filtration system and cleaning method thereof |
| CN107254402A (en) * | 2017-06-27 | 2017-10-17 | 江苏益玛生物科技有限公司 | A kind of agar medium preparation system |
| CN107541532A (en) * | 2017-09-29 | 2018-01-05 | 迪沙药业集团有限公司 | A kind of preparation method of the carboxylic acid of 5 methylpyrazine 2 |
| CN109294889B (en) * | 2018-08-31 | 2021-10-15 | 杭州百芮生物科技有限公司 | Probiotic fermented beverage fermentation tank, component proportion and process method thereof |
| CN110195013A (en) * | 2019-06-21 | 2019-09-03 | 李凤兰 | A kind of anaerobic bacteria culture system |
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