CN106377763A - Multi-purpose sterilizing and clean-keeping spraying agent and application thereof - Google Patents
Multi-purpose sterilizing and clean-keeping spraying agent and application thereof Download PDFInfo
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- CN106377763A CN106377763A CN201610884076.4A CN201610884076A CN106377763A CN 106377763 A CN106377763 A CN 106377763A CN 201610884076 A CN201610884076 A CN 201610884076A CN 106377763 A CN106377763 A CN 106377763A
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- bovine lactoferrin
- lactoferrin
- base material
- material liquid
- ferrum
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/40—Transferrins, e.g. lactoferrins, ovotransferrins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B4/00—General methods for preserving meat, sausages, fish or fish products
- A23B4/14—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12
- A23B4/18—Preserving with chemicals not covered by groups A23B4/02 or A23B4/12 in the form of liquids or solids
- A23B4/20—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/08—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing oxygen, e.g. ethers, acetals, ketones, quinones, aldehydes, peroxides
- A61K47/10—Alcohols; Phenols; Salts thereof, e.g. glycerol; Polyethylene glycols [PEG]; Poloxamers; PEG/POE alkyl ethers
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/46—Ingredients of undetermined constitution or reaction products thereof, e.g. skin, bone, milk, cotton fibre, eggshell, oxgall or plant extracts
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/12—Aerosols; Foams
Abstract
The invention provides a multi-purpose sterilizing and clean-keeping spraying agent which comprises base material liquid, and the base material liquid is formed by mixing bovine lactoferrin and water or formed by mixing combination of bovine lactoferrin and hydrolysates or zymolytes of bovine lactoferrin and water, wherein when the base material liquid is formed by mixing bovine lactoferrin and water, bovine lactoferrin accounts for 0.5-25% of the weight of the base material liquid; when the base material liquid is formed by mixing combination of bovine lactoferrin and hydrolysates or zymolytes of bovine lactoferrin and water, the weight sum of bovine lactoferrin and hydrolysates or zymolytes of bovine lactoferrin accounts for 0.5-25% of the weight of the base material liquid, and the weight ratio of bovine lactoferrin to hydrolysates or zymolytes of bovine lactoferrin is 100:(5-380); and hydrolysates or zymolytes of bovine lactoferrin include one or both of lactoferricin B and lactofettampin. The sterilizing and clean-keeping spraying agent provided by the invention has a broad-spectrum bactericidal property, can be sprayed to the human body without side effects, can not generate drug resistance, has a good therapeutic effect, has better taste and flavor and good patient dependence, is convenient to carry and use, can be sprayed to raw fresh meat to ensure a good preservation effect, and is safe and harmless.
Description
Technical field
The present invention relates to sterilized pharmaceutical techniques field, specifically one kind is with Bovine Lactoferrin and its hydrolysate or enzymolysis
Thing breast ferrum element B（Lactoferricin B, LFcin B）And lactofettampin（LFampin）It is mainly effectively to become etc. peptides
The medicine of the aerosol type of sterilized of part and the formula of cleaning agent and method.
Background technology
Be full of the various antibacterials easily human body being worked the mischief in the environment that people depend on for existence, virus, harmful dust and
Chemicals, their human body especially oral cavities to people, nasal cavity, throat, pudendum, the open sore part at other positions, wound on nipple and body
The hazardness that mouth causes is bigger.How effectively to protect the thing that the position treating these susceptible is people's very concern.Will
The problem carrying out the following several respects of this thing can not be ignored：The medicine that therapeutic scheme is selected should be one, to virus and bacteria wide spectrum
Effectively；Two, have no side effect or few side effects；Three, drug resistance will not be produced；Four, there are preferable mouthfeel fragrance, Bing Renyi
Good from property；Five, carry easy to use etc., have not yet to see very good product so far.In addition fresh meat such as cattle, sheep, pig,
Duck, the fresh-keeping of the fresh meat such as chicken is also required to a kind of safe antistaling agent to healthy non-hazardous.
Numerous studies show, Bovine Lactoferrin is a kind of multi-functional biological activity protein, and it is to multiple micro- both at home and abroad
Biological（Antibacterial, virus, funguses etc.）There are good activity against organisms, be a kind of natural broad-spectrum antibiotic, and body is had
Immunoregulatory activity, can enhancing human body immunity function.Bovine Lactoferrin has the ability of high affine combination ferrum, can be competing with antibacterial
Strive the iron ion in organismic internal environment.And ferrum is its requisite nutrient substance of growth for pathogenic bacterium.Lac Bovis seu Bubali ferrum egg
Have in vain and grab and intercept antibacterial and obtain the effect of ferrum so that antibacterial, virus, funguses etc. are because can not obtain sufficient iron ion
Inactivation or cannot growth and breeding.Bovine Lactoferrin can make bacterial cell surface membrane structure that harmful sexually revising occurs.As Lac Bovis seu Bubali ferrum egg
In vain can be with the lipopolysaccharide in gram-negative bacteria cell wall adventitia（LPS）In conjunction with and make its discharge from adventitia lead to cell wall damage
Wound.Additionally, Bovine Lactoferrin can also be with the Outer membrane protein of escherichia coli, Salmonella typhimurtum（porin）, the bloodthirsty bar of influenza
The colonization factor of bacterium（colonization factor）And the hemoglobin receptor of porphyromonas gingivalis interacts and leads to bacterium
Cell damage is hindered.According to Marieke I.A（2005）Report, Bovine Lactoferrin is gathered around containing two antimicrobial domains area, is claimed respectively
For newborn ferrum element B（Lactoferricin B, LFcin B）And lactofettampin（LFampin）.Author investigation this two kinds
Typical antimicrobial peptide fragment dialogue color candidiasises and the effect of escherichia coli.Look into aobvious through confocal laser scanning microscopy
Show, these peptides can occur cellular uptake, the integrity through freeze-etching transmission electron microscope indicating film simultaneously within a few minutes
Wreck.The most compelling vesicle sample that the uniqueness producing is the discovery that on the Candida albicans Mycoderma being induced by Lfampin
Structure, and the outer membrance separation of escherichia coli that led to by LFcin B peptide and surface elevation [[i]].Domestic Li Tie crystalline substance etc.
（2005）Report [[ii]], the multifunction activity such as antimicrobial antiphlogistic of Bovine Lactoferrin is relevant with the N-terminal region of its high alka, energy
Discharge under acidic pH, the newly-generated antimicrobial peptide containing 25 amino acid residues is referred to as newborn ferrum element.It
It is easy to react with negatively charged antibacterial membrane structure, the combination of peptide can destroy the normal configuration of film.Wherein with newborn ferrum element
The related most important amino acid residue of activity is tryptophan and arginine.Bovine Lactoferrin has Conservative restoration and makees to mucosa
With.Bovine Lactoferrin has immunoregulatory activity, can promote T lymphocyte growth and differentiation.According to external, lactoferrin
Energy and ajor histocompatibility（MHC）The region of protein, and it is present in T4（Helper T cell）And T8（Suppressor T lymphocyte）
CD4 and CD8 on surface determines that base occurs unique combination, and it carries the sequence determining base homology with MHC- II class.Newborn ferrum
Albumen also has the effect adjusting cytokine and lymphokine.As foreign scholar Kuhara（2006）Report [[iii]] recently,
Oral Bovine Lactoferrin can increase the quantity of NK cell in peripheral blood and spleen（In dose dependent）, and increase its α-interference
The yield of element.Experiment also shows that oral Bovine Lactoferrin also can make the level of IL-18 in portal venous circulation increase immediately.This collar extension
Take Bovine Lactoferrin and also can increase and send Ilyushin brief summary（PP）And mesenteric lymph node（MLN）Middle alpha-interferon and the table of beta-interferon
Reach level.In a word, the above results prove that oral Bovine Lactoferrin can stimulate intestinal associated immune function, including generation IL-
18 and interferon type Ⅰ, and strengthen the activity of NK cell.Show through numerous studies, the suppression of the lactoferrin of different ferrum saturations
Bacterium effect is different, empirical tests, and, in the range of 6%-20%, the bacteriostatic activity of lactoferrin is with saturation for the ferrum saturation of lactoferrin
Increase and strengthen.In addition both at home and abroad numerous studies it is also shown that Bovine Lactoferrin and its hydrolysate or zymolyte breast ferrum element B
（Lactoferricin B, LFcin B）And lactofettampin（LFampin）Have higher than Bovine Lactoferrin etc. peptides
200-400 times of broad-spectrum antibacterial and sterilization biological activity.
Due to Bovine Lactoferrin and its hydrolysate or zymolyte breast ferrum element B（Lactoferricin B, LFcin B）With
lactofettampin（LFampin）Etc. the above-mentioned characteristic of peptides, if therefore its rationally application to be realized preferably sterilizing
Clean-keeping function is the present problem needing to solve.
Content of the invention
For the deficiencies in the prior art, the present invention provides a kind of multipurpose bactericial to keep a public place clean spray, one can to oral cavity,
On nasal cavity, throat, pudendum, nipple and body, the open sore part at other positions is treated and is maintained, and it two is capable of to cattle, sheep,
Pig, duck, the fresh meat such as chicken fresh-keeping.
Multipurpose bactericial is kept a public place clean spray, and including base material liquid, described base material liquid is to be mixed with water by Bovine Lactoferrin to form
Base material liquid, or be that the base forming is mixed by the combination of Bovine Lactoferrin, the hydrolysate of Bovine Lactoferrin or zymolyte with water
Feed liquid；When base material liquid is chosen as the former, described Bovine Lactoferrin accounts for the 0.5 ~ 25% of base material liquid weight；When base material liquid is chosen as
During the latter, described Bovine Lactoferrin, the hydrolysate of Bovine Lactoferrin or zymolyte weight sum account for base material liquid weight 0.5 ~
25%, the weight of described Bovine Lactoferrin, the hydrolysate of Bovine Lactoferrin or zymolyte is than for 100：5~380；Described Lac Bovis seu Bubali ferrum egg
White hydrolysate or zymolyte include newborn ferrum element one of B, lactofettampin or the mixing of the two.
Further, described spray also includes essence, gourmet powder and freshener thrin or arbitrarily several mixing
Close；And all it is added to mixing in base material liquid.
Further, the hydrolysate of described Bovine Lactoferrin or zymolyte molecular weight are between 500-8500Da.
Further, the hydrolysate of described Bovine Lactoferrin or zymolyte include newborn ferrum element B, described Bovine Lactoferrin and breast
Ferrum element B weight ratio is for 6-10：0.5-2；Described Bovine Lactoferrin is 5-12 with the mass ratio of newborn ferrum element B weight sum and water:
100.
Further, described lactoferrin is 5 ~ 8% low ferrum saturation lactoferrin for ferrum saturation；Described Lac Bovis seu Bubali ferrum
The lactoferrin of low ferrum saturation is added 800-1000u/g by being combined as of albumen, the hydrolysate of Bovine Lactoferrin or zymolyte
Pepsin carry out digesting the product of 15-25min in 37 DEG C of constant temperature.
The preparation process of described low ferrum saturation lactoferrin is：
1st step, is carried out adsorbing to the lactoferrin in Lac Bovis seu Bubali, is parsed using cation exchange resin；Described resolving is to adopt
Parsed with two grades, first order parsing is the saline solution parsing using 1～2.5%, and desorbed solution is lactoperoxidase pheron, discards；
Second level parsing is the saline solution parsing using 3.5～7%, and desorbed solution is sent into the 2nd step and processed；Described saline solution refer to sodium salt,
Potassium salt or calcium salt soln；
2nd step, the desorbed solution that the 1st step is obtained is concentrated using seperation film；Described seperation film is that molecular cut off exists
The ultrafilter membrane of 1000～30K；
3rd step, the concentrated solution that the 2nd step obtains adopts insoluble Fe3+Chelating resin is adsorbed, then using seperation film to concentration
Liquid is concentrated；Need during described adsorption operations to add acetic acid, acetate and citrate in concentrated solution, prepare its absorption
In environment, citrate concentration is 0.1mol/L ~ 0.5mol/L, and adjusts pH to 5.0～6.7；Described seperation film is retention point
Son amount is in the ultrafilter membrane of 1000～30K.
Further, the concentrated solution that the 2nd described step obtains, can be again through a weak-type sun before sending into the 3rd step
The absorption of ion exchange resin, solution echo the concentration of ultrafilter membrane；Described weak-type cationic resin refers to CM resin.
Further, in the 3rd described step, described insoluble Fe3+Chelating resin refers to D403 chelating resin；Resin
Adsorption time 20～120 minutes, during absorption, rotating speed of agitator speed is 20～150rpm, and adsorption temp is at 3～30 DEG C.
Further, in described 1st step, the preparation process of cation exchange resin is：
1st step, takes 10g chloracetylated polystyrene-divinylbenzene microspheres carrier PS-acyl-Cl, adds 60ml oxolane
Swelling 12 h, add 40ml methanol, then according to weight compares PS-acyl-Cl：Ethylenediamine：Sodium bicarbonate is 1：8：0.7, according to
Secondary addition ethylenediamine and sodium bicarbonate, are stirring reaction 24h at 80 DEG C, after reaction terminates, product are poured in sand core funnel,
It is washed to neutrality with distillation, methanol filter wash is dried under vacuum to constant weight 3 times, obtains crosslinked EDA microsphere supported；
2nd step, claims 60g to take crosslinked EDA microsphere supported, is placed in three-neck flask, after adding swelling 12 h of dimethylformamide, plus
Enter 2- acrylamido -2- methyl propane sulfonic acid 16g, N- butoxy methyl acrylamide 3g, acrylic acid double cyclopentenyl ester,
K2CO35g, tetrabutyl ammonium bromide 12g, under stirring in the oil bath of uniform temperature back flow reaction, reaction terminate after by product
It is transferred in sintered filter funnel, cleaned with the HC1 of 5wt% and after 4 times, use distilled water diafiltration to neutral, finally drained with after methanol filter wash,
It is dried under vacuum to constant weight, obtain cation exchange resin
Above-mentioned multipurpose bactericial is kept a public place clean the application of spray, for suppressing and killing antibacterial and virus.Such as, described spray leads to
Receive and distribute pressure valve to spray for vaporific or solution particulate, for oral cavity, throat, nasal cavity, vagina, pudendum, nipple, male outside
The treatment of the moon, the open sore part of oxter and wound and maintenance, or fresh-keeping for fresh meat.
Sterilized spray in the present invention, has broad-spectrum sterilization, can sparge human body and be free from side effects, will not
Produce drug resistance, therapeutic effect is good, and has preferable mouthfeel fragrance, and patient dependence is good, colleague carries easy to use；Can spray
Mist is on fresh meat, of good preservation effect and safe and harmless.
Specific embodiment
Below in conjunction with specific embodiment, the present invention will be further described, and wherein essence, pigment, sweetener consumption are all
Micro, can slightly adjust according to actual needs.
Embodiment 1：
A kind of multipurpose bactericial is kept a public place clean spray, composed of the following components：
Low ferrum saturation lactoferrin 7g
Borneolum Syntheticum 1.5g
Mentholum 1g
Appropriate sweetener
Olive colour pigment is appropriate
Appropriate essence
Water 100ml
1st, the preparation of low ferrum saturation lactoferrin：Semi-skimmed milk 1890L, pH6.7, total protein is 3.38g/L, lactoferrin
Concentration is 126mg/L, and SPC70 resin is added agitator tank, adsorbs 80min, and stir speed (S.S.) is 60rpm, collects after adsorbing
Milk simultaneously uses RO water rinse resin, adds first order 100L 2.5%NaCl aqueous solution to desorb 40min, speed of agitator 40rpm, receives
RO water wash is used to collect after collected explanations or commentaries attached liquid；Second level 100L 5.5%NaCl aqueous solution is added to desorb 40min, speed of agitator
40rpm, collects resin 3 times with RO water wash after collecting solution attached liquid.The stripping liquid of the second level and second RO leacheate merge
Collect common 180L, be transported in 0.1 μm of microfiltration film device and carry out degerming, remove impurity；Device for ultrafiltration membrane with 30K molecular retention amount
Carry out desalination and concentration, sampling detects that its ferrum saturation is 20%；By lactoferrin dope be input to equipped with CM resin container remove roguing
Albumen, collects the lactoferrin solution after having adsorbed foreign protein and continues to carry out desalination with the device for ultrafiltration membrane of 10K molecular retention amount
Concentrate, a small amount of dope therein is carried out lyophilization gained pale pink dry powder.Powder moisture 3.0%, ash 1.1%, total egg
White is 96.3%, ferrum saturation 20%；Again remaining lactoferrin dope is input to enter equipped with placement D403 chelating resin tank
Row deferrization, adds 0.1mol/L citrate, and pH to 6.0 adjusted by Acetic acid-sodium acetate buffer, in addition stirring and adsorbing iron ion,
Resin adsorption time 120 minutes, rotating speed of agitator speed 60rpm.Collect to adsorb the continuation of the lactoferrin solution after iron ion
Carry out desalination and concentration with the device for ultrafiltration membrane of 10K molecular retention amount, collect dope and carry out lyophilization gained pale pink dry powder.Powder
Last water content 3.0%, ash 1.0%, total protein is 96.3%, ferrum saturation 7%.
2nd, surplus materialss are added in low ferrum saturation lactoferrin.
Embodiment 2
A kind of multipurpose bactericial is kept a public place clean spray, composed of the following components：
Low ferrum saturation lactoferrin 7g
Borneolum Syntheticum 1.5g
Mentholum 1g
Appropriate sweetener
Olive colour pigment is appropriate
Appropriate essence
Water 100ml
1st, the preparation of low ferrum saturation lactoferrin：Semi-skimmed milk 1890L, pH6.7, total protein is 3.38g/L, lactoferrin
Concentration is 126mg/L, and SPC70 resin is added agitator tank, adsorbs 80min, and stir speed (S.S.) is 60rpm, collects after adsorbing
Milk simultaneously uses RO water rinse resin, adds first order 100L 2.5%NaCl aqueous solution to desorb 40min, speed of agitator 40rpm, receives
RO water wash is used to collect after collected explanations or commentaries attached liquid；Second level 100L 5.5%NaCl aqueous solution is added to desorb 40min, speed of agitator
40rpm, collects resin 3 times with RO water wash after collecting solution attached liquid.The stripping liquid of the second level and second RO leacheate merge
Collect common 180L, be transported in 0.1 μm of microfiltration film device and carry out degerming, remove impurity；Device for ultrafiltration membrane with 30K molecular retention amount
Carry out desalination and concentration, sampling detects that its ferrum saturation is 20%；By lactoferrin dope be input to equipped with CM resin container remove roguing
Albumen, collects the lactoferrin solution after having adsorbed foreign protein and continues to carry out desalination with the device for ultrafiltration membrane of 10K molecular retention amount
Concentrate, a small amount of dope therein is carried out lyophilization gained pale pink dry powder.Powder moisture 3.0%, ash 1.1%, total egg
White is 96.3%, ferrum saturation 20%；Again remaining lactoferrin dope is input to enter equipped with placement D403 chelating resin tank
Row deferrization, adds 0.1mol/L citrate, and pH to 6.0 adjusted by Acetic acid-sodium acetate buffer, in addition stirring and adsorbing iron ion,
Resin adsorption time 120 minutes, rotating speed of agitator speed 60rpm.Collect to adsorb the continuation of the lactoferrin solution after iron ion
Carry out desalination and concentration with the device for ultrafiltration membrane of 10K molecular retention amount, collect dope and carry out lyophilization gained pale pink dry powder.Powder
Last water content 3.0%, ash 1.0%, total protein is 96.3%, ferrum saturation 7%.
2nd, the lactoferrin of low ferrum saturation is added the pepsin of 800-1000u/g, 37 DEG C of constant temperature is digested
15min, now lactoferrin and the ratio of newborn ferrum element B are about 7:1.
3rd, surplus materialss are added in lactoferrin plain B mixture with newborn ferrum.
Embodiment 3
A kind of multipurpose bactericial is kept a public place clean spray, composed of the following components：
Low ferrum saturation lactoferrin 9g
Borneolum Syntheticum 2g
Mentholum 1.5g
Appropriate sweetener
Olive colour pigment is appropriate
Appropriate essence
Water 100ml
1st, the preparation of low ferrum saturation lactoferrin：Semi-skimmed milk 1890L, pH6.7, total protein is 3.38g/L, lactoferrin
Concentration is 126mg/L, and SPC70 resin is added agitator tank, adsorbs 80min, and stir speed (S.S.) is 60rpm, collects after adsorbing
Milk simultaneously uses RO water rinse resin, adds first order 100L 2.5%NaCl aqueous solution to desorb 40min, speed of agitator 40rpm, receives
RO water wash is used to collect after collected explanations or commentaries attached liquid；Second level 100L 5.5%NaCl aqueous solution is added to desorb 40min, speed of agitator
40rpm, collects resin 3 times with RO water wash after collecting solution attached liquid.The stripping liquid of the second level and second RO leacheate merge
Collect common 180L, be transported in 0.1 μm of microfiltration film device and carry out degerming, remove impurity；Device for ultrafiltration membrane with 30K molecular retention amount
Carry out desalination and concentration, sampling detects that its ferrum saturation is 20%；By lactoferrin dope be input to equipped with CM resin container remove roguing
Albumen, collects the lactoferrin solution after having adsorbed foreign protein and continues to carry out desalination with the device for ultrafiltration membrane of 10K molecular retention amount
Concentrate, a small amount of dope therein is carried out lyophilization gained pale pink dry powder.Powder moisture 3.0%, ash 1.1%, total egg
White is 96.3%, ferrum saturation 20%；Again remaining lactoferrin dope is input to enter equipped with placement D403 chelating resin tank
Row deferrization, adds 0.1mol/L citrate, and pH to 6.0 adjusted by Acetic acid-sodium acetate buffer, in addition stirring and adsorbing iron ion,
Resin adsorption time 120 minutes, rotating speed of agitator speed 60rpm.Collect to adsorb the continuation of the lactoferrin solution after iron ion
Carry out desalination and concentration with the device for ultrafiltration membrane of 10K molecular retention amount, collect dope and carry out lyophilization gained pale pink dry powder.Powder
Last water content 3.0%, ash 1.0%, total protein is 96.3%, ferrum saturation 7%.
2nd, 37 DEG C of the pepsin constant temperature that the lactoferrin of low ferrum saturation is added 800-1000u/g is digested
20min, now lactoferrin and the ratio of newborn ferrum element B are about 6：1.
3rd, surplus materialss are added in lactoferrin plain B mixture with newborn ferrum.
Embodiment 4
A kind of multipurpose bactericial is kept a public place clean spray, consistent with embodiment 3, differs only in, by SPC70 resin replacement therein
It is that cation exchange resin is prepared by following method：
1st step, takes 10g chloracetylated polystyrene-divinylbenzene microspheres carrier (PS-acyl-Cl), adds 60ml tetrahydrochysene furan
Mutter swelling 12 h, adds 40ml methanol, then according to weight ratio (PS-acyl-Cl)：Ethylenediamine（EDA）：Sodium bicarbonate is 1：
8：0.7, sequentially add ethylenediamine and sodium bicarbonate, be stirring reaction 24h at 80 DEG C, after reaction terminates, product poured into sand
In core funnel, it is washed to neutrality with distillation, methanol filter wash is dried under vacuum to constant weight 3 times, obtains crosslinked EDA microsphere supported；
2nd step, claims 60g to take crosslinked EDA microsphere supported, is placed in three-neck flask, after adding swelling 12 h of dimethylformamide, plus
Enter 2- acrylamido -2- methyl propane sulfonic acid (AMPS) 16g, N- butoxy methyl acrylamide (NBMA) 3g, acrylic acid pair
Cyclopentenes base ester（DCPA）、K2CO35g, tetrabutyl ammonium bromide（TBAB）12g, flows back under stirring in the oil bath of uniform temperature
Reaction, reaction after terminating, product is transferred in sand core funnel, is cleaned with the HC1 of 5wt% and uses distilled water diafiltration extremely after 4 times
Neutrality, is finally drained with after methanol filter wash, is dried under vacuum to constant weight, obtains cation exchange resin.
The technical specification of the low ferrum saturation lactoferrin finally giving is：Powder moisture 3.0%, ash 1.0%, total egg
White is 96.8%, ferrum saturation 6%.
2nd, 37 DEG C of the pepsin constant temperature that the lactoferrin of low ferrum saturation is added 800-1000u/g is digested
25min, now lactoferrin and the ratio of newborn ferrum element B are about 5：1.
3rd, surplus materialss are added in lactoferrin plain B mixture with newborn ferrum.
Multiplex disinfectant spray In vitro Bactericidal Experiments
It is 5000mg/l that In vitro Bactericidal Experiments adopt the concentration of the multipurpose bactericial spray of embodiment 2 preparation, and reference substance is into
The JIEERYIN XIYE that Dou En prestige pharmaceutical Co. Ltd produces；Test strain is：Staphylococcus aureuses ATCC 6538, white are read
Pearl bacterium ATCC 10231, bacillus subtilises ATCC 6633.Test data is as shown in the table：
Extracorporeal disinfecting experiment shows：Multipurpose bactericial spray of keeping a public place clean has very strong bactericidal action to staphylococcus aureuses,
Three kinds of concentration（50mg/l、100mg/l、200mg/l）Lower effect 5 minutes, sterilizing rate can reach more than 99%；To bacillus subtilis
Bacterium also has certain lethal effect, three kinds of concentration（50mg/l、100mg/l、200mg/l）Under, sterilizing rate can reach more than 90%；
There are stronger lethal effect, three kinds of concentration to Candida albicans（50mg/l、100mg/l、200mg/l）Lower effect 10 minutes,
Sterilizing rate averagely can reach more than 98.5%.
Compare test for embodiment one, two, three, four, experimental result is as shown in the table, Comparatively speaking embodiment 4
Best results：
Claims (10)
1. a kind of multipurpose bactericial keep a public place clean spray it is characterised in that：Including base material liquid, described base material liquid is by Bovine Lactoferrin
Mix the base material liquid of composition with water, or be by the combination of Bovine Lactoferrin, the hydrolysate of Bovine Lactoferrin or zymolyte and water
The base material liquid of mixing composition；When base material liquid is chosen as the former, described Bovine Lactoferrin accounts for the 0.5 ~ 25% of base material liquid weight；When
When base material liquid is chosen as the latter, described Bovine Lactoferrin, the hydrolysate of Bovine Lactoferrin or zymolyte weight sum account for base material liquid
The 0.5 ~ 25% of weight, the weight of described Bovine Lactoferrin, the hydrolysate of Bovine Lactoferrin or zymolyte is than for 100：5~380；Institute
State the hydrolysate of Bovine Lactoferrin or zymolyte includes newborn ferrum element one of B, lactofettampin or the mixing of the two.
2. multipurpose bactericial according to claim 1 keep a public place clean spray it is characterised in that：Described spray also includes perfume (or spice)
Essence, gourmet powder and freshener thrin or arbitrarily several mixing, and all it is added to mixing in base material liquid.
3. multipurpose bactericial according to claim 1 keep a public place clean spray it is characterised in that：The hydrolysis of described Bovine Lactoferrin
Thing or zymolyte molecular weight are between 500-8500Da.
4. multipurpose bactericial according to claim 1 keep a public place clean spray it is characterised in that：The hydrolysis of described Bovine Lactoferrin
Thing or zymolyte include newborn ferrum element B, and described Bovine Lactoferrin and newborn ferrum element B weight ratio is for 6-10：0.5-2；Described Lac Bovis seu Bubali ferrum egg
The white mass ratio with breast ferrum element B weight sum and water is 5-12:100.
5. according to the arbitrary described multipurpose bactericial of claim 1-4 keep a public place clean spray it is characterised in that：Described Bovine Lactoferrin
For described lactoferrin for ferrum saturation be 5 ~ 8% low ferrum saturation lactoferrin；Described Bovine Lactoferrin, Bovine Lactoferrin
Hydrolysate or zymolyte be combined as the lactoferrin of low ferrum saturation is added the pepsin of 800-1000u/g in constant temperature
37 DEG C carry out digesting the product of 15-25min.
6. multipurpose bactericial according to claim 5 keep a public place clean spray it is characterised in that：Described low ferrum saturation breast ferrum egg
White preparation process is：
1st step, is carried out adsorbing to the lactoferrin in Lac Bovis seu Bubali, is parsed using cation exchange resin；Described resolving is to adopt
Parsed with two grades, first order parsing is the saline solution parsing using 1～2.5%, and desorbed solution is lactoperoxidase pheron, discards；
Second level parsing is the saline solution parsing using 3.5～7%, and desorbed solution is sent into the 2nd step and processed；Described saline solution refer to sodium salt,
Potassium salt or calcium salt soln；
2nd step, the desorbed solution that the 1st step is obtained is concentrated using seperation film；Described seperation film is that molecular cut off exists
The ultrafilter membrane of 1000～30K；
3rd step, the concentrated solution that the 2nd step obtains adopts insoluble Fe3+Chelating resin is adsorbed, then using seperation film to concentrated solution
Concentrated；Need during described adsorption operations to add acetic acid, acetate and citrate in concentrated solution, prepare it and adsorb ring
In border, citrate concentration is 0.1mol/L ~ 0.5mol/L, and adjusts pH to 5.0～6.7；Described seperation film is retention molecule
Amount is in the ultrafilter membrane of 1000～30K.
7. multipurpose bactericial according to claim 6 keeps a public place clean spray it is characterised in that in described the 3rd step, described
Insoluble Fe3+Chelating resin refers to D403 chelating resin；The adsorption time of resin 20～120 minutes, rotating speed of agitator during absorption
Speed is 20～150rpm, and adsorption temp is at 3～30 DEG C.
8. multipurpose bactericial according to claim 6 keeps a public place clean spray it is characterised in that in described 1st step, cation is handed over
The preparation process changing resin is：
1st step, takes 10g chloracetylated polystyrene-divinylbenzene microspheres carrier PS-acyl-Cl, adds 60ml oxolane
Swelling 12 h, add 40ml methanol, then according to weight compares PS-acyl-Cl：Ethylenediamine：Sodium bicarbonate is 1：8：0.7, according to
Secondary addition ethylenediamine and sodium bicarbonate, are stirring reaction 24h at 80 DEG C, after reaction terminates, product are poured in sand core funnel,
It is washed to neutrality with distillation, methanol filter wash is dried under vacuum to constant weight 3 times, obtains crosslinked EDA microsphere supported；
2nd step, claims 60g to take crosslinked EDA microsphere supported, is placed in three-neck flask, after adding swelling 12 h of dimethylformamide, plus
Enter 2- acrylamido -2- methyl propane sulfonic acid 16g, N- butoxy methyl acrylamide 3g, acrylic acid double cyclopentenyl ester,
K2CO35g, tetrabutyl ammonium bromide 12g, under stirring in the oil bath of uniform temperature back flow reaction, reaction terminate after by product
It is transferred in sintered filter funnel, cleaned with the HC1 of 5wt% and after 4 times, use distilled water diafiltration to neutral, finally drained with after methanol filter wash,
It is dried under vacuum to constant weight, obtain cation exchange resin.
9. the arbitrary described multipurpose bactericial of claim 1-8 keep a public place clean spray application it is characterised in that being used for suppressing and killing
Go out antibacterial and virus.
10. multipurpose bactericial according to claim 9 keep a public place clean spray application it is characterised in that：Described spray leads to
Receive and distribute pressure valve to spray for vaporific or solution particulate, for oral cavity, throat, nasal cavity, vagina, pudendum, nipple, male outside
The treatment of the moon, the open sore part of oxter and wound and maintenance, or fresh-keeping for fresh meat.
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| CN201610884076.4A CN106377763B (en) | 2016-10-11 | 2016-10-11 | Multi-purpose sterilizing and cleaning spray and its application |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1840179A (en) * | 2006-01-19 | 2006-10-04 | 方雅悯 | Application of bovine lactoferricin in preparation of medicine for treating HP infected gastric disease |
| CN101247823A (en) * | 2005-05-05 | 2008-08-20 | 布里斯托尔-迈尔斯斯奎布公司 | Use of bovine lactoferrin in the manufacture of a medicament for inhibiting the growth of bacteria |
| CN105999236A (en) * | 2016-07-06 | 2016-10-12 | 方雅悯 | Medicament for treating bovine mastitis and application thereof |
-
2016
- 2016-10-11 CN CN201610884076.4A patent/CN106377763B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101247823A (en) * | 2005-05-05 | 2008-08-20 | 布里斯托尔-迈尔斯斯奎布公司 | Use of bovine lactoferrin in the manufacture of a medicament for inhibiting the growth of bacteria |
| CN1840179A (en) * | 2006-01-19 | 2006-10-04 | 方雅悯 | Application of bovine lactoferricin in preparation of medicine for treating HP infected gastric disease |
| CN105999236A (en) * | 2016-07-06 | 2016-10-12 | 方雅悯 | Medicament for treating bovine mastitis and application thereof |
Non-Patent Citations (3)
| Title |
|---|
| KRAAN, M. I. A. VAN D等: "Ultrastructural effects of antimicrobial peptides from bovine lactoferrin on the membranes of Candida albicans and Escherichia coli", 《PEPTIDES》 * |
| MAMORUTOMITA等: "Potent Antibacterial Peptides Generated by Pepsin Digestion of Bovine Lactoferrin", 《JOURNAL OF DAIRY SCIENCE》 * |
| 张东送等: "酶解牛乳铁蛋白制备乳铁素B的酶解条件研究", 《中国乳品工业》 * |
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